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Pesquisa : D13.695.578.424.450.275 [Categoria DeCS]
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Id: biblio-1130108
Autor: Tomaseto, Alex Augusto; Alpiste, Marcel Costa; Nassar, Alessandra Figueiredo de Castro; Destéfano, Suzete Aparecida Lanza.
Título: Antibacterial activity of phytopathogenic Streptomyces strains against bacteria associated to clinical diseases / Atividade antimicrobiana de Streptomyces fitopatogênicas contra bactérias associadas a doenças de importância clínica
Fonte: Arq. Inst. Biol;87:e0142020, 2020. tab.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil; . Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Resumo: The genus Streptomyces is associated with the ability to produce and excrete a variety of bioactive compounds, such as antibiotic, antifungal and antiviral. Biological active polyketide and peptide compounds with applications in medicine, agriculture and biochemical research are synthesized by PKS-I and NRPS genes. The evaluation of the presence of these genes associated with the biosynthesis of secondary metabolites in different phytopathogenic Streptomyces strains were performed using degenerated primers. The positive signal was observed in 58/63 Streptomyces strains for NRPS gene, 43/63 for PKS-I, and for PKS-II all the 63 strains showed positive signal of amplification. These strains also were tested with double layer agar-well technique against bacterial with clinical importance, and it was possible to observe the Streptomyces spp. strains were able to inhibit the growth of 14, 20, 13 and 3 isolates Gram-positive and Gram-negative bacteria, Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 14579), Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 11775) respectively. The Streptomyces sp. strains IBSBF 2019 and IBSBF 2397 showed antibacterial activity against all four bacteria-target tested.(AU)

O gênero Streptomyces apresenta alta capacidade de produzir e excretar uma grande variedade de compostos biologicamente ativos, como antibióticos, antifúngicos e antivirais. Compostos biologicamente ativos de policetídeos e peptídeos com aplicações na medicina, agricultura e pesquisas bioquímicas são sintetizados pelos genes PKS-I e NRPS. A avaliação da presença desses genes associados à biossíntese de metabólitos secundários em diferentes linhagens de Streptomyces fitopatogênicas foi realizada através do uso de primers degenerados. O sinal positivo foi observado em 58/63 linhagens de Streptomyces para o gene NRPS, 43/63 para o gene PKS-I e, para o gene PKS-II, todas as 63 linhagens apesentaram o sinal positivo de amplificação. Essas linhagens também foram testadas através da técnica de dupla camada contra bactérias de importância clínica e foi possível observar que as linhagens de Streptomyces spp. foram capazes de inibir o crescimento de 14, 20, 13 e 3 isolados de bactérias Gram-positivas e Gram-negativas, Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 14579), Pseudomonas aeruginosa (ATCC 27853) e Escherichia coli (ATCC 11775), respectivamente. As linhagens de Streptomyces sp. ISBSF 2019 e 2397 apresentaram atividade antibacteriana contra todas as bactérias-alvo testadas.(AU)
Descritores: Pseudomonas aeruginosa/crescimento & desenvolvimento
Staphylococcus aureus/crescimento & desenvolvimento
Streptomyces/metabolismo
Bacillus cereus/crescimento & desenvolvimento
Escherichia coli/crescimento & desenvolvimento
Antibacterianos/metabolismo
-Peptídeo Sintases/genética
Streptomyces/genética
Amplificação de Genes
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Primers do DNA
Policetídeo Sintases/genética
Antibacterianos/farmacologia
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Id: biblio-828119
Autor: de Filippis, Ivano; Andrade, Claudia Ferreira de; Caldeira, Nathalia; Azevedo, Aline Carvalho de; Almeida, Antonio Eugenio de.
Título: Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical samples
Fonte: Braz. j. infect. dis;20(4):335-341, July-Aug. 2016. tab, graf.
Idioma: en.
Projeto: INCQS/FIOCRUZ; . FAPERJ.
Resumo: Abstract Background Several in-house PCR-based assays have been described for the detection of bacterial meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae from clinical samples. PCR-based methods targeting different bacterial genes are frequently used by different laboratories worldwide, but no standard method has ever been established. The aim of our study was to compare different in-house and a commercial PCR-based tests for the detection of bacterial pathogens causing meningitis and invasive disease in humans. Methods A total of 110 isolates and 134 clinical samples (99 cerebrospinal fluid and 35 blood samples) collected from suspected cases of invasive disease were analyzed. Specific sets of primers frequently used for PCR-diagnosis of the three pathogens were used and compared with the results achieved using the multiplex approach described here. Several different gene targets were used for each microorganism, namely ctrA, crgA and nspA for N. meningitidis, ply for S. pneumoniae, P6 and bexA for H. influenzae. Results All used methods were fast, specific and sensitive, while some of the targets used for the in-house PCR assay detected lower concentrations of genomic DNA than the commercial method. An additional PCR reaction is described for the differentiation of capsulated and non-capsulated H. influenzae strains, the while commercial method only detects capsulated strains. Conclusions The in-house PCR methods here compared showed to be rapid, sensitive, highly specific, and cheaper than commercial methods. The in-house PCR methods could be easily adopted by public laboratories of developing countries for diagnostic purposes. The best results were achieved using primers targeting the genes nspA, ply, and P6 which were able to detect the lowest DNA concentrations for each specific target.
Descritores: Haemophilus influenzae/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
Meningite por Haemophilus/diagnóstico
Meningite Meningocócica/diagnóstico
Meningite Pneumocócica/diagnóstico
Neisseria meningitidis/isolamento & purificação
-Streptococcus pneumoniae/isolamento & purificação
Streptococcus pneumoniae/genética
DNA Bacteriano/genética
Haemophilus influenzae/genética
Sensibilidade e Especificidade
Primers do DNA
Meningite por Haemophilus/microbiologia
Meningite Meningocócica/microbiologia
Meningite Pneumocócica/microbiologia
Neisseria meningitidis/genética
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Belone, Andrea de Faria Fernandes
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Id: biblio-839189
Autor: Azevedo, Michelle de Campos Soriani; Ramuno, Natália Mortari; Fachin, Luciana Raquel Vincenzi; Tassa, Mônica; Rosa, Patrícia Sammarco; Belone, Andrea de Faria Fernandes; Diório, Suzana Madeira; Soares, Cleverson Teixeira; Garlet, Gustavo Pompermaier; Trombone, Ana Paula Favaro.
Título: qPCR detection of Mycobacterium leprae in biopsies and slit skin smear of different leprosy clinical forms
Fonte: Braz. j. infect. dis;21(1):71-78, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Abstract Leprosy, whose etiological agent is Mycobacterium leprae, is a chronic infectious disease that mainly affects the skin and peripheral nervous system. The diagnosis of leprosy is based on clinical evaluation, whereas histopathological analysis and bacilloscopy are complementary diagnostic tools. Quantitative PCR (qPCR), a current useful tool for diagnosis of infectious diseases, has been used to detect several pathogens including Mycobacterium leprae. The validation of this technique in a robust set of samples comprising the different clinical forms of leprosy is still necessary. Thus, in this study samples from 126 skin biopsies (collected from patients on all clinical forms and reactional states of leprosy) and 25 slit skin smear of leprosy patients were comparatively analyzed by qPCR (performed with primers for the RLEP region of M. leprae DNA) and routine bacilloscopy performed in histological sections or in slit skin smear. Considering clinical diagnostic as the gold standard, 84.9% of the leprosy patients were qPCR positive in skin biopsies, resulting in 84.92% sensitivity, with 84.92 and 61.22% positive (PPV) and negative (NPV) predictive values, respectively. Concerning bacilloscopy of histological sections (BI/H), the sensitivity was 80.15% and the PPV and NPV were 80.15 and 44.44%, respectively. The concordance between qPCR and BI/H was 87.30%. Regarding the slit skin smear, 84% of the samples tested positive in the qPCR. Additionally, qPCR showed 100% specificity, since all samples from different mycobacteria, from healthy individuals, and from other granulomatous diseases presented negative results. In conclusion, the qPCR technique for detection of M. leprae using RLEP primers proved to be specific and sensitive, and qPCR can be used as a complementary test to diagnose leprosy irrespective of the clinical form of disease.
Descritores: Pele/microbiologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
Hanseníase/microbiologia
Mycobacterium leprae/isolamento & purificação
-Valores de Referência
Pele/patologia
Biópsia
DNA Bacteriano/isolamento & purificação
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Primers do DNA/isolamento & purificação
Hanseníase/patologia
Mycobacterium leprae/genética
Limites: Humanos
Tipo de Publ: Estudo de Validação
Responsável: BR1.1 - BIREME


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Id: biblio-888925
Autor: Camilo, Lilian Muniz; Pereira-Chioccola, Vera Lucia; Gava, Ricardo; Meira-Strejevitch, Cristina da Silva; Vidal, Jose Ernesto; Mattos, Cinara Cássia Brandão de; Frederico, Fábio Batista; De Mattos, Luiz Carlos; Spegiorin, Lígia Cosentino Junqueira Franco; Murata, Fernando Henrique Antunes; Ferreira, Marina Neves; Barbosa, Deusenia Machado Ulisses; Gonçalves, Fausto da Silva; Dias, Cristiane Moraes; Catelan, Marcia Wakai; Siqueira, Rubens Camargo; Previato, Mariana; Barbosa, Amanda Pires; Cavallini, Danilo.
Título: Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in São Paulo, Brazil
Fonte: Braz. j. infect. dis;21(6):638-647, Nov.-Dec. 2017. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de Sao Paulo; . Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: ABSTRACT Symptomatic forms of toxoplasmosis are a serious public health problem and occur in around 10-20% of the infected people. Aiming to improve the molecular diagnosis of symptomatic toxoplasmosis in Brazilian patients, this study evaluated the performance of real time PCR testing two primer sets (B1 and REP-529) in detecting Toxoplasma gondii DNA. The methodology was assayed in 807 clinical samples with known clinical diagnosis, ELISA, and conventional PCR results in a 9-year period. All samples were from patients with clinical suspicion of several features of toxoplasmosis. According to the minimum detection limit curve (in CT), REP-529 had greater sensitivity to detect T. gondii DNA than B1. Both primer sets were retrospectively evaluated using 515 DNA from different clinical samples. The 122 patients without toxoplasmosis provided high specificity (REP-529, 99.2% and B1, 100%). From the 393 samples with positive ELISA, 146 had clinical diagnosis of toxoplasmosis and positive conventional PCR. REP-529 and B1 sensitivities were 95.9% and 83.6%, respectively. Comparison of REP-529 and B1 performances was further analyzed prospectively in 292 samples. Thus, from a total of 807 DNA analyzed, 217 (26.89%) had positive PCR with, at least one primer set and symptomatic toxoplasmosis confirmed by clinical diagnosis. REP-529 was positive in 97.23%, whereas B1 amplified only 78.80%. After comparing several samples in a Brazilian referral laboratory, this study concluded that REP-529 primer set had better performance than B1 one. These observations were based after using cases with defined clinical diagnosis, ELISA, and conventional PCR.
Descritores: Toxoplasma/genética
Toxoplasmose/diagnóstico
-Toxoplasmose/classificação
Estudos Prospectivos
Estudos Retrospectivos
DNA de Protozoário/genética
Sensibilidade e Especificidade
Primers do DNA/genética
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1124206
Autor: Li, Bin; Lin, Furong; Huang, Ping; Guo, Wenying; Zheng, Yongqi.
Título: Development of nuclear SSR and chloroplast genome markers in diverse Liriodendron chinense germplasm based on low-coverage whole genome sequencing
Fonte: Biol. Res;53:21, 2020. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . National Forest and Grass Germplasm Resources Bank Program.
Resumo: BACKGROUND: Liriodendron chinense ranges widely in subtropical China and northern Vietnam; however, it inhabits several small, isolated populations and is now an endangered species due to its limited seed production. The objective of this study was to develop a set of nuclear SSR (simple sequence repeats) and multiple chloroplast genome markers for genetic studies in L. chinense and their characterization in diverse germplasm. RESULTS: We performed low-coverage whole genome sequencing of the L. chinense from four genotypes, assembled the chloroplast genome and identified nuclear SSR loci by searching in contigs for SSR motifs. Comparative analysis of the four chloroplast genomes of L. chinense revealed 45 SNPs, 17 indels, 49 polymorphic SSR loci, and five small inversions. Most chloroplast intraspecific polymorphisms were located in the interspaces of single-copy regions. In total, 6147 SSR markers were isolated from low-coverage whole genome sequences. The most common SSR motifs were dinucleotide (70.09%), followed by trinucleotide motifs (23.10%). The motif AG/TC (33.51%) was the most abundant, followed by TC/AG (25.53%). A set of 13 SSR primer combinations were tested for amplification and their ability to detect polymorphisms in a set of 109 L. chinense individuals, representing distinct varieties or germplasm. The number of alleles per locus ranged from 8 to 28 with an average of 21 alleles. The expected heterozygosity (He) varied from 0.19 to 0.93 and the observed heterozygosity (Ho) ranged from 0.11 to 0.79. CONCLUSIONS: The genetic resources characterized and tested in this study provide a valuable tool to detect polymorphisms in L. chinense for future genetic studies and breeding programs.
Descritores: Polimorfismo Genético/genética
Genoma de Planta/genética
Liriodendron/genética
Genoma de Cloroplastos/genética
-Primers do DNA/genética
DNA de Plantas/genética
Repetições de Microssatélites
Alelos
Sequenciamento Completo do Genoma
Genótipo
Responsável: CL1.1 - Biblioteca Central


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Mazzafera, Paulo
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Id: biblio-886905
Autor: LLERENA, JUAN P P; ARAÚJO, PEDRO; MAZZAFERA, PAULO.
Título: Optimization of RT-PCR reactions in studies with genes of lignin biosynthetic route in Saccharum spontaneum
Fonte: An. acad. bras. ciênc;90(1):509-519, Mar. 2018. tab, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; . Fundação de Amparo à Pesquisa do estado de São Paulo; . Conselho Nacional de Desenvolvimento Científico e Tecnológico; . FAPESP.
Resumo: ABSTRACT Saccharum spontaneum has been used for the development of energy cane a crop aimed to be used for the production of second-generation ethanol, or lignocellulosic ethanol. Lignin is a main challenge in the conversion of cell wall sugars into ethanol. In our studies to isolate the genes the lignin biosynthesis in S. spontaneum we have had great difficulty in RT-PCR reactions. Thus, we evaluated the effectiveness of different additives in the amplification of these genes. While COMT and CCoAOMT genes did not need any additives for other genes there was no amplification (HCT, F5H, 4CL and CCR) or the yield was very low (CAD and C4H). The application of supplementary cDNA was enough to overcome the non-specificity and low yield for C4H and C3H, while the addition of 0.04% BSA + 2% formamide was effective to amplify 4CL, CCR, F5H and CCR. HCT was amplified only by addition of 0.04% BSA + 2% formamide + 0.1 M trehalose and amplification of PAL was possible with addition of 2% of DMSO. Besides optimization of expression assays, the results show that additives can act independently or synergistically.
Descritores: Regulação da Expressão Gênica de Plantas/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Técnicas de Amplificação de Ácido Nucleico/métodos
Saccharum/genética
-Parede Celular/genética
Primers do DNA
Etanol
Lignina/biossíntese
Lignina/genética
Metiltransferases/genética
Responsável: BR1.1 - BIREME


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Id: biblio-886917
Autor: PASSAMANI, PAULO Z; CARVALHO, CARLOS R; SOARES, FERNANDA A F.
Título: Protocol for chromosome-specific probe construction using PRINS, micromanipulation and DOP-PCR techniques
Fonte: An. acad. bras. ciênc;90(1):41-47, Mar. 2018. graf.
Idioma: en.
Resumo: ABSTRACT Chromosome-specific probes have been widely used in molecular cytogenetics, being obtained with different methods. In this study, a reproducible protocol for construction of chromosome-specific probes is proposed which associates in situ amplification (PRINS), micromanipulation and degenerate oligonucleotide-primed PCR (DOP-PCR). Human lymphocyte cultures were used to obtain metaphases from male and female individuals. The chromosomes were amplified via PRINS, and subcentromeric fragments of the X chromosome were microdissected using microneedles coupled to a phase contrast microscope. The fragments were amplified by DOP-PCR and labeled with tetramethyl-rhodamine-5-dUTP. The probes were used in fluorescent in situ hybridization (FISH) procedure to highlight these specific regions in the metaphases. The results show one fluorescent red spot in male and two in female X chromosomes and interphase nuclei.
Descritores: Reação em Cadeia da Polimerase/métodos
Primers do DNA/genética
Marcação in Situ com Primers/métodos
Análise Citogenética/métodos
-Sondas de DNA/genética
Reprodutibilidade dos Testes
Hibridização in Situ Fluorescente/métodos
Cromossomos Humanos X/genética
Microdissecção/métodos
Limites: Humanos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-951563
Autor: Almeida, R P; Stouthamer, R.
Título: Phylogeny of the Trichogramma endosymbiont Wolbachia, an alpha-proteobacteria (Rickettsiae) / Filogenia do endosimbionte Wolbachia em Trichogramma, an alpha-proteobacteria (Rickettsiae)
Fonte: Braz. j. biol;78(3):421-428, Aug. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Wolbachia (Hertig) endosymbionts are extensively studied in a wide range of organisms and are known to be transmitted through the egg cytoplasm to the offsping. Wolbachia may cause several types of reproductive modifications in arthropods. In Trichogramma species, parthenogenesis-inducing Wolbachia bacteria allow females wasps to produce daughters from unfertilized eggs and these bacteria are present in at least 9% of all Trichogramma species. Phylogenetic studies have led to the subdivision of the Wolbachia clade in five supergroups (A, B, C, D and E) and Wolbachia from Trichogramma belong to supergroup B. Here, using the wsp gene, four groups of Wolbachia that infect Trichogramma species were distinguished and the addition of a new group "Ato" was suggested due to the addition of Wolbachia from Trichogramma atopovirilia (Oatman and Platner). Specific primers were designed and tested for the "Ato" group. Seventy-five percent of all evaluated Wolbachia strains from Trichogramma fell within "Sib" group.

Resumo Endosimbiontes do gênero Wolbachia (Hertig) são extensivamente estudados em uma ampla gama de organismos e são conhecidos por serem transmitidos via citoplasma do ovo hospedeiro para seu descendente. Wolbachia pode causar vários tipos de alterações reprodutivas nos artrópodes. Nas espécies de Trichogramma, a reprodução partenogenética induzida por Wolbachia, possibilita as fêmeas dos parasitoides a produção de fêmeas a partir de ovos não fertilizados e estas bactérias estão presentes em pelo menos 9% de todas as espécies de Trichogramma. Estudos filogenéticos têm levado a subdivisão do clado Wolbachia em cinco supergrupos (A, B, C, D and E). Wolbachia em Trichogramma pertence ao supergrupo B. Com o gene wsp foi possível se distinguir quatro grupos de Wolbachia que infectam Trichogramma e adicionar um novo grupo (Ato) devido a inclusão de Wolbachia detectada em Trichogramma atopovirilia (Oatman and Platner, 1983). Primers específicos foram construídos e testados para o grupo "Ato". Setenta e cinco por cento de todas as linhagens de Wolbachia que infectam Trichogramma se enquadraram dentro do grupo "Sib".
Descritores: Proteínas da Membrana Bacteriana Externa/metabolismo
Vespas/microbiologia
Primers do DNA/genética
Alphaproteobacteria/metabolismo
Wolbachia/genética
Genes Bacterianos/genética
-Filogenia
Reprodução
Especificidade da Espécie
Simbiose
Vespas/genética
Limites: Animais
Feminino
Responsável: BR1.1 - BIREME


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Barbosa, Maria Luísa
Id: lil-242489
Autor: Barbosa, Maria Luisa.
Título: Detecçäo e tipagem de vírus dengue sorotipos 1 e 2 por multiplex RT-PCR / Multiplex RT-PCR used for detection and tipification of dengue viruses sorotypes 1 and 2
Fonte: Rev. Inst. Adolfo Lutz;58(1):79-83, 1999. ilus.
Idioma: pt.
Resumo: Os vírus Dengue säo arbovírus da família Flaviviridae, com 4 sorotipos antigenicamente distintos. Variaçöes genéticas intraespecíficas entre os mesmos sorotipos, inclusive em uma mesma epidemia, estäo bem estabelecidas. O desenvolvimento da técnica de polimenizaçäo em cadeia (PCR) é uma alternativa na identificaçäo dos vírus Dengue. Neste estudo construímos dois novos pares de primers para os sorotipos 1 e 2, sem a ajuda de programas de computador. Foram utilizadas como modelo as seqüências genômicas de DEN-1 (Western Pacific), Nauru Island e Den-2 New Guinea C. Os primers sense 5' ACA AAA AGT GGA GAC CTG GGC TC 3'(DIS) e complementar 5'GTC TAT TCC AAG TCT CTT GGG 3' (DIA) correspondem respectivamente às posições 769 a 791 e 1607 a 1587 do genoma do vírus DEN-1. Estas seqüência reconhecem parte das regiões de membrana (M) e proteína estrutural (E) do genoma do sorotipos 1 e contem 838 pares de bases. A seqüência de primers de DEN-2 foi 5' TGA AGG GGA CGG TTC TCC ATG 3' (D2S) homólogos aos nucleotídios 1838 a 1859 e 5' GAC TCC CAC CAA TAC TAG TGA CAC 3' (D2A) correspondendo às posições 2312 a 2288. O produto da amplificaçäo foi de 474 pares de bases correspondendo a uma porçäo do genoma responsável pela síntese da proteína E. A especificidade dos primers foi avaliada por multiplex RT-PCR
Descritores: Sorotipagem
Vírus da Dengue/isolamento & purificação
-RNA Viral
Primers do DNA
Dengue/diagnóstico
Responsável: BR91.2 - Centro de Documentação


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Texto completo
Id: lil-588898
Autor: Cabral, Luciana Sanches; Festa Neto, Cyro; Sanches Júnior, José A; Ruiz, Itamar R. G.
Título: Genomic instability in human actinic keratosis and squamous cell carcinoma
Fonte: Clinics;66(4):523-528, 2011. ilus, tab.
Idioma: en.
Resumo: OBJECTIVE: To compare the repetitive DNA patterns of human actinic keratoses and squamous cell carcinomas to determine the genetic alterations that are associated with malignant transformation. INTRODUCTION: Cancer cells are prone to genomic instability, which is often due to DNA polymerase slippage during the replication of repetitive DNA and to mutations in the DNA repair genes. The progression of benign actinic keratoses to malignant squamous cell carcinomas has been proposed by several authors. MATERIAL AND METHODS: Eight actinic keratoses and 24 squamous cell carcinomas (SCC), which were pair-matched to adjacent skin tissues and/or leucocytes, were studied. The presence of microsatellite instability (MSI) and the loss of heterozygosity (LOH) in chromosomes 6 and 9 were investigated using nine PCR primer pairs. Random Amplified Polymorphic DNA patterns were also evaluated using eight primers. RESULTS: MSI was detected in two (D6S251, D9S50) of the eight actinic keratosis patients. Among the 8 patients who had squamous cell carcinoma-I and provided informative results, a single patient exhibited two LOH (D6S251, D9S287) and two instances of MSI (D9S180, D9S280). Two LOH and one example of MSI (D6S251) were detected in three out of the 10 patients with squamous cell carcinoma-II. Among the four patients with squamous cell carcinoma-III, one patient displayed three MSIs (D6S251, D6S252, and D9S180) and another patient exhibited an MSI (D9S280). The altered random amplified polymorphic DNA ranged from 70 percent actinic keratoses, 76 percent squamous cell carcinoma-I, and 90 percent squamous cell carcinoma-II, to 100 percent squamous cell carcinoma-III. DISCUSSION: The increased levels of alterations in the microsatellites, particularly in D6S251, and the random amplified polymorphic DNA fingerprints were statistically significant in squamous cell carcinomas, compared with actinic keratoses. CONCLUSION: The overall alterations that were observed in the repetitive DNA of actinic keratoses and squamous cell carcinomas indicate the presence of a spectrum of malignant progression.
Descritores: Carcinoma de Células Escamosas/genética
Primers do DNA/genética
Ceratose Actínica/genética
Perda de Heterozigosidade/genética
Instabilidade de Microssatélites
Neoplasias Cutâneas/genética
-Transformação Celular Neoplásica/genética
Transformação Celular Neoplásica/patologia
CHROMOSOMES, HUMAN, PAIR ABDOMEN, ACUTE
Cromossomos Humanos Par 9
Impressões Digitais de DNA
Progressão da Doença
Ceratose Actínica/patologia
Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde