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Id: biblio-950724
Autor: Shahrani, Masoud; Dehkordi, Farhad Safarpoor; Momtaz, Hassan.
Título: Characterization of Escherichia coli virulence genes, pathotypes and antibiotic resistance properties in diarrheic calves in Iran
Fonte: Biol. Res;47:1-13, 2014. tab.
Idioma: en.
Resumo: BACKGROUND: Calf diarrhea is a major economic concern in bovine industry all around the world. This study was carried out in order to investigate distribution of virulence genes, pathotypes, serogroups and antibiotic resistance properties of Escherichia coli isolated from diarrheic calves. RESULTS: Totally, 76.45% of 824 diarrheic fecal samples collected from Isfahan, Chaharmahal, Fars and Khuzestan provinces, Iran were positive for E. coli and all of them were also positive for cnf2, hlyA, cdtIII, f17c, lt, st, stx1, eae, ehly, stx2 and cnf1 virulence genes. Chaharmahal had the highest prevalence of STEC (84.61%), while Isfahan had the lowest (71.95%). E. coli serogroups had the highest frequency in 1-7 days old calves and winter season. Distribution of ETEC, EHEC, AEEC and NTEC pathotypes among E. coli isolates were 28.41%, 5.07%, 29.52% and 3.49%, respectively. Statistical analyses were significant for presence of bacteria between various seasons and ages. All isolates had the high resistance to penicillin (100%), streptomycin (98.25%) and tetracycline (98.09%) antibiotics. The most commonly detected resistance genes were aadA1, sul1, aac[3]-IV, CITM, and dfrA1. The most prevalent serogroup among STEC was O26. CONCLUSIONS: Our findings should raise awareness about antibiotic resistance in diarrheic calves in Iran. Clinicians should exercise caution when prescribing antibiotics.
Descritores: Doenças dos Bovinos/microbiologia
Proteínas de Escherichia coli/genética
Farmacorresistência Bacteriana/genética
Diarreia/veterinária
Escherichia coli/patogenicidade
-Estações do Ano
Virulência/genética
Resistência ao Cloranfenicol/genética
Sorotipagem
Reação em Cadeia da Polimerase
Prevalência
Fatores Etários
Primers do DNA
Diarreia/microbiologia
Diarreia/epidemiologia
Escherichia coli/efeitos dos fármacos
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Sorogrupo
Irã (Geográfico)/epidemiologia
Limites: Animais
Bovinos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  2 / 170 LILACS  
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Id: lil-785796
Autor: Romeiro, Marilia Farignoli; Souza, William Marciel de; Tolardo, Aline Lavado; Vieira, Luiz Carlos; Colombo, Tatiana Elias; Aquino, Victor Hugo; Nogueira, Maurício Lacerda; Figueiredo, Luiz Tadeu Moraes.
Título: Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil
Fonte: Rev. Soc. Bras. Med. Trop;49(3):279-285tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo; . CNPq.
Resumo: Abstract: INTRODUCTION: The genus Flavivirus includes several pathogenic species that cause severe illness in humans. Therefore, a rapid and accurate molecular method for diagnosis and surveillance of these viruses would be of great importance. Here, we evaluate and optimize a quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) method for the diagnosis of the Flavivirus genus. METHODS: We evaluated different commercial kits that use the SYBR Green system for real-time RT-PCR with a primer set that amplifies a fragment of the NS5 flavivirus gene. The specificity and sensitivity of the assay were tested using twelve flaviviruses and ribonucleic acid (RNA) transcribed from the yellow fever virus. Additionally, this assay was evaluated using the sera of 410 patients from different regions of Brazil with acute febrile illness and a negative diagnosis for the dengue virus. RESULTS: The real-time RT-PCR amplified all flaviviruses tested at a melting temperature of 79.92 to 83.49°C. A detection limit of 100 copies per ml was determined for this assay. Surprisingly, we detected dengue virus in 4.1% (17/410) of samples from patients with febrile illness and a supposedly negative dengue infection diagnosis. The viral load in patients ranged from 2.1×107to 3.4×103copies per ml. CONCLUSIONS: The real-time RT-PCR method may be very useful for preliminary diagnoses in screenings, outbreaks, and other surveillance studies. Moreover, this assay can be easily applied to monitor viral activity and to measure viral load in pathogenesis studies.
Descritores: Infecções por Flavivirus/diagnóstico
Flavivirus/genética
-Compostos Orgânicos
Kit de Reagentes para Diagnóstico
Brasil
RNA Viral/genética
Sensibilidade e Especificidade
Infecções por Flavivirus/virologia
Primers do DNA
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Flavivirus/isolamento & purificação
Flavivirus/classificação
Corantes Fluorescentes
Limites: Humanos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  3 / 170 LILACS  
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Id: biblio-950749
Autor: Yu, Guangchao; Chen, Lei; Lin, Chii-wann; Li, Bing; Cui, Hemiao; Chen, Siyi; Miao, Jian; Bian, Huawei; Chen, Dingqiang; Deng, Yang.
Título: Loop-mediated isothermal amplification assays for screening of bacterial integrons
Fonte: Biol. Res;47:1-10, 2014. ilus, tab.
Idioma: en.
Projeto: National 973-Plan of China; . International Science & Technology Cooperation Program; . Science and Technology Planning Project of Guangdong Province, China; . National Natural Science Foundation of China; . National Science and Technology Support Program; . National Outstanding Doctoral Dissertation Funding; . Guangdong Outstanding Doctoral Dissertation Funding; . China Postdoctoral Science Foundation funded; . Fundamental Research Funds for the Central Universities; . Fund for Outstanding Youth of Anhui Academy of Agricultural Sciences.
Resumo: BACKGROUND: The occurrence and prevalence of integrons in clinical microorganisms and their role played in antimicrobial resistance have been well studied recently. As screening and detection of integrons are concerned, current diagnostic methodologies are restricted by significant drawbacks and novel methods are required for integrons detection. RESULTS: In this study, three loop-mediated isothermal amplification (LAMP) assays targeting on class 1, 2 and 3 integrons were implemented and evaluated. Optimization of these detection assays were performed, including studing on the reaction temperature, volume, time, sensitivity and specificity (both primers and targets). Application of the established LAMP assays were further verified on a total of 1082 isolates (previously identified to be 397 integron-positive and 685 integron-negative strains). According to the results, the indispensability of each primer had been confirmed and the optimal reaction temperature, volume and time were found to be 65°C, 45 min and 25 µL, respectively. As application was concerned, 361, 28 and 8 isolates carrying intI1, intI2 and intI3 yielded positive amplicons, respectively. Other 685 integron-negative bacteria were negative for the integron-screening LAMP assays, totaling the detection rate and specificity to be 100%. CONCLUSIONS: The intI1-, intI2- and intI3-LAMP assays established in this study were demonstrated to be the valid and rapid detection methodologies for the screening of bacterial integrons.
Descritores: DNA Bacteriano/isolamento & purificação
Técnicas de Amplificação de Ácido Nucleico/métodos
Integrons
-Compostos Orgânicos
Salmonella/genética
Serratia marcescens/genética
Staphylococcus/genética
Vibrio cholerae/genética
Contagem de Colônia Microbiana
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase/métodos
Sensibilidade e Especificidade
DNA Complementar
Primers do DNA
Integrases/genética
Farmacorresistência Bacteriana/genética
Eletroforese em Gel de Ágar
Escherichia coli/genética
Corantes Fluorescentes
Temperatura Alta
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-896994
Autor: Esposito, Danillo Lucas Alves; Fonseca, Benedito Antonio Lopes da.
Título: Sensitivity and detection of chikungunya viral genetic material using several PCR-based approaches
Fonte: Rev. Soc. Bras. Med. Trop;50(4):465-469, July-Aug. 2017. tab, graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: Chikungunya fever is a condition resulting from infection by chikungunya virus (CHIKV), an Aedes sp.-transmitted virus. This disease has been diagnosed in thousands of cases in the Americas, particularly in Brazil, in recent years, and there is an ongoing epidemic of chikungunya fever in Brazil that began in 2014. Clinical diagnosis is difficult; only a few cases have been confirmed by laboratory tests due to the low number of specific, efficient tests available for virus or antibody detection. Here, we aimed to evaluate different polymerase chain reaction (PCR) approaches for detection of CHIKV genetic material. METHODS: Specific primers and probes within the viral capsid gene region were designed for this work. To evaluate the analytic sensitivity of detection, human sera were spiked with serial dilutions of the viral stock. Several PCR protocols were performed to investigate the sensitivity of CHIKV RNA detection in serum dilutions ranging from 106 to 1 PFU equivalents. RESULTS: The technique showing the greatest sensitivity was a real-time PCR assay using specific probes that could detect the genetic material of the virus at all dilutions, followed by conventional PCR. Digital PCR showed low sensitivity and was much more expensive than other technologies. Digital PCR should be used for specific purposes other than clinical diagnosis. CONCLUSIONS: Although quantitative PCR using probes was more expensive than the use of intercalating dyes or conventional PCR, it had the highest sensitivity out of all tested PCR approaches.
Descritores: RNA Viral/análise
Vírus Chikungunya/genética
Primers do DNA/genética
Febre de Chikungunya/diagnóstico
-Sensibilidade e Especificidade
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1041406
Autor: Benacer, Douadi; Zain, Siti Nursheena Mohd; Lewis, John W; Khalid, Mohd Khairul Nizam Mohd; Thong, Kwai Lin.
Título: A duplex endpoint PCR assay for rapid detection and differentiation of leptospira strains
Fonte: Rev. Soc. Bras. Med. Trop;50(2):239-242, Mar.-Apr. 2017. tab, graf.
Idioma: en.
Projeto: Malaya High Impact Research Grant; . University of Malaya.
Resumo: Abstract INTRODUCTION: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. METHODS: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively. RESULTS: The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water. CONCLUSIONS: Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.
Descritores: DNA Bacteriano/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
Primers do DNA
Leptospira/classificação
-Especificidade da Espécie
Sensibilidade e Especificidade
Leptospira/isolamento & purificação
Leptospira/genética
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: biblio-950756
Autor: Palomino, Jaime; Herrera, Giannina; Dettleff, Phillip; Martínez, Víctor.
Título: Growth differentiation factor 9 and bone morphogenetic protein 15 expression in previtellogenic oocytes and during early embryonic development of Yellow-tail Kingfish Seriola lalandi
Fonte: Biol. Res;47:1-7, 2014. graf, tab.
Idioma: en.
Projeto: CONICYT; . FONDECYT; . Chilean Aquaculture Diversification Grant.
Resumo: BACKGROUND: During fish oocyte maturation, specific molecules are expressed and accumulated within oocyte until fertilization and embryo development. Special attention have been paid in members of the transforming growth factor (TGF-ß) superfamily; growth differentiation factor 9 (GDF9/gdf9) and bone morphogenetic protein 15 (BMP15/bmp15), which exert regulatory functions during oocyte maturation and follicle development. However, little attention has been paid to the involvement of these molecules during embryogenesis considering its importance for the formation of a good quality egg and subsequent embryo survival. The purpose of this study was to analyze the expression of gdf9 andbmp15 in previtellogenic oocytes and during early embryonic development in Seriola lalandi, a pelagic fish with increasing prospect for its aquaculture development, which however, show high mortality at embryo and larval stages. RESULTS: Through RT-qPCR it was found that gdf9 expression was higher in previtellogenic oocytes decreasing after ovulation. This expression profile agrees with its participation in early stages of the follicular development. The transcripts for bmp15 also showed the highest levels in previtellogenic oocytes, however this expression was lower than obtained with gdf9. Conversely, in recently spawned oocytes mRNA bmp15 levels were highest than observed to gdf9. This, is consequent with the main role proposed for this growth factor at the final fish oocyte maturation: avoid the ovulation of an immature oocyte. During embryo development, low levels of mRNA were detected to gdf9, with an increase in 48 H post-fertilization embryos. The bmp15 expression did not change throughout development and was higher than gdf9 at 16 cells, blastula and appearance embryos stages. CONCLUSIONS: Both (gdf9 and bmp15) expression profiles in previtellogenic oocytes and newly spawned eggs are consistent with the described functions for these growth factors in vertebrate ovarian physiology in early and late stages of the follicular development. So, these genes could be considered as quality biomarkers at these stages. However, further studies of these proteins throughout folliculogenesis, are necessaries to fully understand their functions during the oocyte formation. In addition, the persistent expression of these growth factors during development, allows us to speculate possible roles in embryonic processes, which must also be addressed.
Descritores: Oócitos/metabolismo
Vitelogênese/fisiologia
Perciformes/embriologia
Proteína Morfogenética Óssea 15/metabolismo
Fator 9 de Diferenciação de Crescimento/metabolismo
-Transcrição Genética/fisiologia
Perciformes/classificação
RNA Mensageiro/isolamento & purificação
RNA Mensageiro/metabolismo
Biomarcadores/análise
DNA Complementar/análise
Primers do DNA
Desenvolvimento Embrionário/genética
Reação em Cadeia da Polimerase em Tempo Real
Peixes/embriologia
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950759
Autor: Fakruddin, Md; Rahaman, Mizanur; Ahmed, Monzur Morshed; Hoque, Md Mahfuzul.
Título: Stress tolerant virulent strains of Cronobacter sakazakii from food
Fonte: Biol. Res;47:1-12, 2014. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The aim of this study was to isolate and characterize virulent strains of Cronobacter sakazakii from food samples of Bangladesh. RESULT: Six (6) Cronobacter sakazakii was isolated and identified from 54 food samples on the basis of biochemical characteristics, sugar fermentation, SDS-PAGE of whole cell protein, plasmid profile and PCR of Cronobacter spp. specific genes (esak, gluA, zpx, ompA, ERIC, BOX-AIR) and sequencing. These strains were found to have moderately high antibiotic resistance against common antibiotics and some are ESBL producer. Most of the C. sakazakii isolates were capable of producing biofilm (strong biofilm producer), extracellular protease and siderophores, curli expression, haemolysin, haemagglutinin, mannose resistant haemagglutinin, had high cell surface hydrophobicity, significant resistance to human serum, can tolerate high concentration of salt, bile and DNase production. Most of them produced enterotoxins of different molecular weight. The isolates pose significant serological cross-reactivity with other gram negative pathogens such as serotypes of Salmonella spp., Shigella boydii, Shigella sonnei, Shigella flexneri and Vibrio cholerae. They had significant tolerance to high temperature, low pH, dryness and osmotic stress. CONCLUSION: Special attention should be given in ensuring hygiene in production and post-processing to prevent contamination of food with such stress-tolerant virulent Cronobacter sakazakii.
Descritores: Estresse Fisiológico/fisiologia
Cronobacter sakazakii/fisiologia
Leite/microbiologia
Microbiologia de Alimentos
-Bangladesh
Virulência
DNA Bacteriano/análise
Resistência a Tetraciclina/genética
Reação em Cadeia da Polimerase/métodos
Especiarias/microbiologia
Sideróforos/metabolismo
Análise de Sequência de DNA
Primers do DNA
Reações Cruzadas
Cronobacter sakazakii/isolamento & purificação
Cronobacter sakazakii/classificação
Cronobacter sakazakii/patogenicidade
Leite/classificação
Eletroforese em Gel de Poliacrilamida
Fermentação/fisiologia
Temperatura Alta
Concentração de Íons de Hidrogênio
Antibacterianos/farmacologia
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


  8 / 170 LILACS  
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Id: biblio-950778
Autor: Muzaffar, Adnan; Kiani, Sarfraz; Khan, Muhammad Azmat Ullah; Rao, Abdul Qayyum; Ali, Arfan; Awan, Mudassar Fareed; Iqbal, Adnan; Nasir, Idrees Ahmad; Shahid, Ahmad Ali; Husnain, Tayyab.
Título: Chloroplast localization of Cry1Ac and Cry2A protein- an alternative way of insect control in cotton
Fonte: Biol. Res;48:1-11, 2015. ilus, tab.
Idioma: en.
Resumo: BACKGROUND: Insects have developed resistance against Bt-transgenic plants. A multi-barrier defense system to weaken their resistance development is now necessary. One such approach is to use fusion protein genes to increase resistance in plants by introducing more Bt genes in combination. The locating the target protein at the point of insect attack will be more effective. It will not mean that the non-green parts of the plants are free of toxic proteins, but it will inflict more damage on the insects because they are at maximum activity in the green parts of plants. RESULTS: Successful cloning was achieved by the amplification of Cry2A, Cry1Ac, and a transit peptide. The appropriate polymerase chain reaction amplification and digested products confirmed that Cry1Ac and Cry2A were successfully cloned in the correct orientation. The appearance of a blue color in sections of infiltrated leaves after 72 hours confirmed the successful expression of the construct in the plant expression system. The overall transformation efficiency was calculated to be 0.7%. The amplification of Cry1Ac-Cry2A and Tp2 showed the successful integration of target genes into the genome of cotton plants. A maximum of 0.673 µg/g tissue of Cry1Ac and 0.568 µg/g tissue of Cry2A was observed in transgenic plants. We obtained 100% mortality in the target insect after 72 hours of feeding the 2nd instar larvae with transgenic plants. The appearance of a yellow color in transgenic cross sections, while absent in the control, through phase contrast microscopy indicated chloroplast localization of the target protein. CONCLUSION: Locating the target protein at the point of insect attack increases insect mortality when compared with that of other transgenic plants. The results of this study will also be of great value from a biosafety point of view.
Descritores: Proteínas de Bactérias/genética
Proteínas Recombinantes de Fusão
Cloroplastos/genética
Controle de Insetos/métodos
Gossypium/genética
Endotoxinas/genética
Proteínas Hemolisinas/genética
Lepidópteros
-Bacillus thuringiensis
Proteínas de Bactérias/análise
Resistência a Inseticidas/genética
Imuno-Histoquímica
Expressão Gênica/genética
Cloroplastos/metabolismo
Reação em Cadeia da Polimerase
Microscopia de Contraste de Fase
Plantas Geneticamente Modificadas
Clonagem Molecular
Primers do DNA
Folhas de Planta/genética
Transgenes/fisiologia
Endotoxinas/análise
Fusão Gênica
Proteínas Hemolisinas/análise
Inseticidas
Larva
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  9 / 170 LILACS  
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Id: biblio-1124206
Autor: Li, Bin; Lin, Furong; Huang, Ping; Guo, Wenying; Zheng, Yongqi.
Título: Development of nuclear SSR and chloroplast genome markers in diverse Liriodendron chinense germplasm based on low-coverage whole genome sequencing
Fonte: Biol. Res;53:21, 2020. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . National Forest and Grass Germplasm Resources Bank Program.
Resumo: BACKGROUND: Liriodendron chinense ranges widely in subtropical China and northern Vietnam; however, it inhabits several small, isolated populations and is now an endangered species due to its limited seed production. The objective of this study was to develop a set of nuclear SSR (simple sequence repeats) and multiple chloroplast genome markers for genetic studies in L. chinense and their characterization in diverse germplasm. RESULTS: We performed low-coverage whole genome sequencing of the L. chinense from four genotypes, assembled the chloroplast genome and identified nuclear SSR loci by searching in contigs for SSR motifs. Comparative analysis of the four chloroplast genomes of L. chinense revealed 45 SNPs, 17 indels, 49 polymorphic SSR loci, and five small inversions. Most chloroplast intraspecific polymorphisms were located in the interspaces of single-copy regions. In total, 6147 SSR markers were isolated from low-coverage whole genome sequences. The most common SSR motifs were dinucleotide (70.09%), followed by trinucleotide motifs (23.10%). The motif AG/TC (33.51%) was the most abundant, followed by TC/AG (25.53%). A set of 13 SSR primer combinations were tested for amplification and their ability to detect polymorphisms in a set of 109 L. chinense individuals, representing distinct varieties or germplasm. The number of alleles per locus ranged from 8 to 28 with an average of 21 alleles. The expected heterozygosity (He) varied from 0.19 to 0.93 and the observed heterozygosity (Ho) ranged from 0.11 to 0.79. CONCLUSIONS: The genetic resources characterized and tested in this study provide a valuable tool to detect polymorphisms in L. chinense for future genetic studies and breeding programs.
Descritores: Polimorfismo Genético/genética
Genoma de Planta/genética
Liriodendron/genética
Genoma de Cloroplastos/genética
-Primers do DNA/genética
DNA de Plantas/genética
Repetições de Microssatélites
Alelos
Sequenciamento Completo do Genoma
Genótipo
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950830
Autor: Zhang, Yuhong; Wu, Hongsheng; Xie, Jiaqin; Jiang, Ruixin; Deng, Congshuang; Pang, Hong.
Título: Transcriptome responses to heat- and cold-stress in ladybirds (Cryptolaemus montrouzieri Mulasnt) analyzed by deep-sequencing
Fonte: Biol. Res;48:1-14, 2015. graf, tab.
Idioma: en.
Projeto: National Basic Research Program of China; . National Natural Science Foundation of China; . National Natural Science Foundation of China; . Chinese Academy of Sciences; . Science and Technology Planning Project of Guangdong Province. Knowledge Innovation Program.
Resumo: BACKGROUND: Changed temperature not only threaten agricultural production, but they also affect individual biological behavior, population and community of many insects, and consequently reduce the stability of our ecosystem. Insect's ability to respond to temperature stress evolved through a complex adaptive process, thus resulting in varied temperature tolerance among different insects. Both high and low extreme temperatures are detrimental to insect development since they constitute an important abiotic stress capable of inducing abnormal biological responses. Many studies on heat or cold tolerance of ladybirds have focused on measurements of physiological and biochemical indexes such as supercooling point, higher/lower lethal temperatures, survival rate, dry body weight, water content, and developmental duration. And studies of the molecular mechanisms of ladybird responses to heat or cold stress have focused on single genes, such as those encoding heat shock proteins, but has not been analyzed by transcriptome profiling. RESULTS: In this study, we report the use of Digital Gene Expression (DGE) tag profiling to gain insight into transcriptional events associated with heat- and cold-stress in C. montrouzieri. About 6 million tags (49 bp in length) were sequenced in a heat stress group, a cold stress group and a negative control group. We obtained 687 and 573 genes that showed significantly altered expression levels following heat and cold shock treatments, respectively. Analysis of the global gene expression pattern suggested that 42 enzyme-encoding genes mapped to many Gene Ontology terms are associated with insect's response to heat- and cold-stress. CONCLUSIONS: These results provide a global assessment of genes and molecular mechanisms involved in heat and cold tolerance.
Descritores: Besouros/genética
Resposta ao Choque Térmico/genética
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Resposta ao Choque Frio/genética
Transcriptoma
-Estresse Fisiológico/genética
Besouros/classificação
Besouros/enzimologia
Biblioteca Gênica
Análise de Sequência de DNA/métodos
Genes de Insetos/fisiologia
Temperatura Baixa
Primers do DNA
Perfilação da Expressão Gênica/métodos
Reação em Cadeia da Polimerase em Tempo Real
Ontologia Genética
Temperatura Alta
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central



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