Base de dados : LILACS
Pesquisa : D20.777.351 [Categoria DeCS]
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Fotocópia
Id: lil-335979
Autor: Inda, Ana M; García, Adriana L; Errecalde, Ana L; Badrán, Amado F.
Título: Effect of tissue and plasma factors on kidney proliferation
Fonte: Biocell;21(1):13-18, Apr. 1997.
Idioma: en.
Resumo: Liver extract, plasma from intact mice, ES2 tumour extract and plasma from tumour bearing mice has an inhibiting effect on the mitotic activity of hepatocytes and duodenal enterocytes. In the present experiments, the effect of these treatments on the mitotic activity of renal tubular cells was studied. C3HS 28 day-old male mice, standardized for periodicity analysis were used. The determination of normal mitotic circadian curve of the renocytes was done. A second batch of mice were injected with 0.01 ml/gr of either liver extract, plasma from intact mice, ES2 tumour extract or plasma from tumour bearing mice, at 16:00 hours and controlled at 08:00, 12:00 and 16:00 hs during 2 consecutive days post treatment. Colchicine (2 micrograms/gr) was injected 4 hours before killing. Kidneys were processed for histology and mitotic index determinations. Results were expressed as colchicine metaphases per 1000 nuclei, and showed that mitotic activity values of treated animals were significantly lower than those of controls. In conclusion, mitotic activity inhibition of renocytes may be due to some non specific plasmatic and/or tissue factors.
Descritores: Plasma
Extratos de Tecidos
Túbulos Renais/citologia
-CAMUNDONGOS ENDOGAMICOS CABATTOIRSH
Divisão Celular/efeitos dos fármacos
Extratos Hepáticos/farmacologia
Fator de Crescimento de Hepatócito/farmacologia
Mitose
Neoplasias Experimentais
Extratos de Tecidos
Túbulos Renais/efeitos dos fármacos
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME


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Fotocópia
Id: lil-209647
Autor: Surur, Jose M; Badran, Amado F.
Título: Accion del extracto de higado de ratones parcialmente hepatectomizados sobre la actividad mitotica de los enterocitos del raton joven / Action of liver extract from partially hepatectomized mice on the mitotic activity of young mouse enterocytes
Fonte: Medicina (B.Aires);57(3):315-9, 1997. tab, graf.
Idioma: es.
Resumo: En un trabajo anterior demonstramos que el plasma de retones adultos obtenido 36 horas post hepatectomía parcial, ejerce un efecto inhibitorio en la actividad mitótica de los enterocitos crípticos duodenales del ratón joven. En el presente trabajo se analiza la posibilidad de que dicho efecto se origine en algún factor del hígado regenerante. Para ello se estudia la acción del extracto de hígado de ratones adultos (90 días) obtenido 36 horas después de su hepatectomía parcial (70 por ciento), sobre la actividad mitótica de los enterocitos de ratones jóvenes, analizando 3 niveles celulares de las criptas duodenales. Se emplearon 36 ratones hembra de la cepa C3H/S de 27 días de edad la mitad de los cuales recibió, a las 16:00 horas, una inyección intraperitoneal de solución fisiológica, y restantes extracto hepático (0,01 ml/g). Lotes de 8 animales de cada grupo se sacrificaron a las 08:00/16, 12:00/20 y 16:00/24 (hora del día/horas post tratamiento) previa inyección de colchicina (2mug/g) 4 horas antes. Los resultados, expresados como metafases colchicínas por mil núcleos, demuenstran que la actividad mitótica, en los animales tratados con extracto, es significativamente menor con respecto a los testigos. El efecto inhibidor se manifesta en los niveles celulares de 1 a 4 y de 5 a 12 células de las criptas analizadas. En el nivel superior, de 13 a 20 células, no se aprecia ninguna modificación de la actividad proliferativa. Esta inhibición de la actividad mitótica de los enterocitos de las zonas basal y media de las criptas duodenales es probablemente debido a factores hepáticos difusibles.
Descritores: Duodeno/citologia
Hepatectomia
Técnicas In Vitro
Extratos Hepáticos/farmacologia
Mitose/efeitos dos fármacos
-Camundongos Endogâmicos C3H
Limites: Camundongos
Animais
Responsável: BR1.1 - BIREME


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Fotocópia
Id: lil-109005
Autor: Campos de Carvalho, A. C; Eiras, L. A; Waltzman, M; Hertzberg, E. L; Spray, D. C.
Título: Properties of channels from rat liver gap junction membrane fractions incorporated into planar lipid bilayers
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;25(1):81-92, 1992. tab.
Idioma: en.
Resumo: Rat membrane fractions highly enriched for gap junctions can be incorporated into planar lipid bilayers exhibiting channel currents with both voltage-dependent and independent components. Voltage dependence, however, is only one of the characteristics of liver gap junction channels. Other features include poor ionic selectivity and sensitivity to calcium, pH, octanol and to some intracellularly applied antibodies. To further test the junctional nature of channels from membrane fractions highly enriched in gap junctions incorporated into lipid bilayers we studied the sensitivity of these channels to uncoupling agents and determined channel selectivity properties. We found the incorporated channels to be insensitive to calcium and octanol, and in most cases to pH in the range of 5-7, suggesting that either these agents do not interact directly with the junctional channels or that the corresponding gating regions are inactivated during the isolation and reconstitution procedures. Attempts to block channel activity using polyclonal and monoclonal connexin 32 antibodies were generally unsuccessful, although one antibody (a monoclonal directed against the carboxy terminus portion of connexin32) blocked channel activity. Selectivity measurements indicated that the incorporated channels were slightly cation selective (PNa=Pk > PCl) and were permeable to large ions. These results further support the idea that functional connexin32 gap junction channels are present in channel activity recorded from rat liver junctional membranes incorporated into planar bilayers
Descritores: Junções Intercelulares/fisiologia
Bicamadas Lipídicas/fisiologia
Extratos Hepáticos/fisiologia
-Condutividade Elétrica
Limites: Ratos
Animais
Responsável: BR26.1 - Biblioteca Central



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