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Id: biblio-984697
Autor: Ramírez-Carreto, Santos; Pérez-García, Erick I; Salazar-García, Sandra I; Bernáldez-Sarabia, Johanna; Licea-Navarro, Alexei; Rudiño-Piñera, Enrique; Pérez-Martínez, Leonor; Pedraza-Alva, Gustavo; Rodríguez-Almazán, Claudia.
Título: Identification of a pore-forming protein from sea anemone Anthopleura dowii Verrill (1869) venom by mass spectrometry
Fonte: J. venom. anim. toxins incl. trop. dis;25:e147418, 2019. graf, ilus.
Idioma: en.
Projeto: CONACyT; . PAPIIT; . CONACyT; . CAPES; . CNPq; . CAPES.
Resumo: Background: Pore-forming proteins (PFP) are a class of toxins abundant in the venom of sea anemones. Owing to their ability to recognize and permeabilize cell membranes, pore-forming proteins have medical potential in cancer therapy or as biosensors. In the present study, we showed the partial purification and sequencing of a pore-forming protein from Anthopleura dowii Verrill (1869). 17. Methods: Cytolytic activity of A. dowii Verrill (1869) venom was determined via hemolysis assay in the erythrocytes of four mammals (sheep, goat, human and rabbit). The cytotoxic activity was analyzed in the human adherent lung carcinoma epithelial cells (A549) by the cytosolic lactate dehydrogenase (LDH) assay, and trypan blue staining. The venom was fractionated via ammonium sulfate precipitation gradient, dialysis, and ion exchange chromatography. The presence of a pore-forming protein in purified fractions was evaluated through hemolytic and cytotoxic assays, and the activity fraction was analyzed using the percent of osmotic protections after polyethylene glycol (PEG) treatment and mass spectrometry. 18. Results: The amount of protein at which the venom produced 50% hemolysis (HU50) was determined in hemolysis assays using erythrocytes from sheep (HU50 = 10.7 ± 0.2 µg), goat (HU50 = 13.2 ± 0.3 µg), rabbit (HU50 = 34.7 ± 0.5 µg), and human (HU50 = 25.6 ± 0.6 µg). The venom presented a cytotoxic effect in A549 cells and the protein amount present in the venom responsible for producing 50% death (IC50) was determined using a trypan blue cytotoxicity assay (1.84 ± 0.40 µg/mL). The loss of membrane integrity in the A549 cells caused by the venom was detected by the release of LDH in proportion to the amount of protein. The venom was fractionated; and the fraction with hemolytic and cytotoxic activities was analyzed by mass spectrometry. A pore-forming protein was identified. The cytotoxicity in the A549 cells produced by the fraction containing the pore-forming protein was osmotically protected by PEG-3350 Da molecular mass, which corroborated that the loss of integrity in the plasma membrane was produced via pore formation. 19. Conclusion: A. dowii Verrill (1869) venom contains a pore-forming protein suitable for designing new drugs for cancer therapy.(AU)
Descritores: Anêmonas-do-Mar
Venenos de Cnidários/isolamento & purificação
Neoplasias Pulmonares/terapia
-Venenos/toxicidade
Espectrometria de Massas/métodos
Células A549
Limites: Humanos
Animais
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: biblio-954854
Autor: Oliveira, Cláudia S; Caldeira, Cleópatra A. S; Diniz-Sousa, Rafaela; Romero, Dolores L; Marcussi, Silvana; Moura, Laura A; Fuly, André L; Carvalho, Cicília de; Cavalcante, Walter L. G; Gallacci, Márcia; Pai, Maeli Dal; Zuliani, Juliana P; Calderon, Leonardo A; Soares, Andreimar M.
Título: Pharmacological characterization of cnidarian extracts from the Caribbean Sea: evaluation of anti-snake venom and antitumor properties
Fonte: J. venom. anim. toxins incl. trop. dis;24:22, 2018. ilus, graf.
Idioma: en.
Projeto: Ministry of Science and Technology (MCTI); . National Council for Scientific and Technological Development (CNPq); . Funding Authority for Studies and Projects (FINEP); . Coordination for the Improvement of Higher Education Personnel (CAPES); . National Research Network in Marine Biotechnology; . Genetic Heritage Management Board (CGEN/MMA).
Resumo: Cnidarians produce toxins, which are composed of different polypeptides that induce pharmacological effects of biotechnological interest, such as antitumor, antiophidic and anti-clotting activities. This study aimed to evaluate toxicological activities and potential as antitumor and antiophidic agents contained in total extracts from five cnidarians: Millepora alcicornis, Stichodactyla helianthus, Plexaura homomalla, Bartholomea annulata and Condylactis gigantea (total and body wall). Methods: The cnidarian extracts were evaluated by electrophoresis and for their phospholipase, proteolytic, hemorrhagic, coagulant, fibrinogenolytic, neuromuscular blocking, muscle-damaging, edema-inducing and cytotoxic activities. Results: All cnidarian extracts showed indirect hemolytic activity, but only S. helianthus induced direct hemolysis and neurotoxic effect. However, the hydrolysis of NBD-PC, a PLA2 substrate, was presented only by the C gigantea (body wall) and S. helianthus. The extracts from P. homomalla and S. helianthus induced edema, while only C gigantea and S. helianthus showed intensified myotoxic activity. The proteolytic activity upon casein and fibrinogen was presented mainly by B. annulata extract and all were unable to induce hemorrhage or fibrinogen coagulation. Cnidarian extracts were able to neutralize clotting induced by Bothrops jararacussu snake venom, except M. alcicornis. All cnidarian extracts were able to inhibit hemorrhagic activity induced by Bothrops moojeni venom. Only the C. gigantea (body wall) inhibited thrombin-induced coagulation. All cnidarian extracts showed antitumor effect against Jurkat cells, of which C. gigantea (body wall) and S. helianthus were the most active; however, only C. gigantea (body wall) and M. alcicornis were active against B16F10 cells. Conclusion: The cnidarian extracts analyzed showed relevant in vitro inhibitory potential over the activities induced by Bothrops venoms; these results may contribute to elucidate the possible mechanisms of interaction between cnidarian extracts and snake venoms.(AU)
Descritores: Antivenenos/toxicidade
Venenos de Cnidários/farmacologia
Venenos de Crotalídeos/imunologia
-Bothrops
Neoplasias/imunologia
Limites: Animais
Masculino
Ratos
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: biblio-894176
Autor: Lazcano-Pérez, Fernando; Zavala-Moreno, Ariana; Rufino-González, Yadira; Ponce-Macotela, Martha; García-Arredondo, Alejandro; Cuevas-Cruz, Miguel; Gómez-Manzo, Saúl; Marcial-Quino, Jaime; Arreguín-Lozano, Barbarín; Arreguín-Espinosa, Roberto.
Título: Hemolytic, anticancer and antigiardial activity of Palythoa caribaeorum venom
Fonte: J. venom. anim. toxins incl. trop. dis;24:12, 2018. graf, ilus.
Idioma: en.
Projeto: PAPIIT; . Consejo Nacional de Ciencia y Tecnología (CONACyT).
Resumo: Cnidarian venoms and extracts have shown a broad variety of biological activities including cytotoxic, antibacterial and antitumoral effects. Most of these studied extracts were obtained from sea anemones or jellyfish. The present study aimed to determine the toxic activity and assess the antitumor and antiparasitic potential of Palythoa caribaeorum venom by evaluating its in vitro toxicity on several models including human tumor cell lines and against the parasite Giardia intestinalis. Methods: The presence of cytolysins and vasoconstrictor activity of P. caribaeorum venom were determined by hemolysis, PLA2 and isolated rat aortic ring assays, respectively. The cytotoxic effect was tested on HCT-15 (human colorectal adenocarcinoma), MCF-7 (human mammary adenocarcinoma), K562 (human chronic myelogenous leukemia), U251 (human glyoblastoma), PC-3 (human prostatic adenocarcinoma) and SKLU-1 (human lung adenocarcinoma). An in vivo toxicity assay was performed with crickets and the antiparasitic assay was performed against G. intestinalis at 24 h of incubation. Results: P. caribaeorum venom produced hemolytic and PLA2 activity and showed specific cytotoxicity against U251 and SKLU-1 cell lines, with approximately 50% growing inhibition. The venom was toxic to insects and showed activity against G. intestinalis in a dose-dependent manner by possibly altering its membrane osmotic equilibrium. Conclusion: These results suggest that P. caribaeorum venom contains compounds with potential therapeutic value against microorganisms and cancer.(AU)
Descritores: Giardíase/terapia
Giardia lamblia/parasitologia
Venenos de Cnidários/antagonistas & inibidores
Venenos de Cnidários/toxicidade
-Anticarcinógenos
Ratos Wistar
Venenos de Cnidários/uso terapêutico
Hemolíticos
Limites: Animais
Masculino
Ratos
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: biblio-1024982
Autor: Lecanda, María Julieta; Guagliardo, Silvia; Siebenhaar, Paula; Vera, Pablo; Cardinali, Sandra; Tanzola, R. Daniel.
Título: Ecoepidemiología de las lesiones causadas por medusas urticantes en el balneario de Monte Hermoso. Buenos Aires. Argentina / Ecoepidemiology of lesions caused by venomous jellyfish in Monte Hermoso beach. Buenos Aires. Argentina
Fonte: Rev. Asoc. Med. Bahía Blanca;29(1):21-29, enero-marzo 2019.
Idioma: es.
Resumo: En la costa sudoeste bonaerense uno de los principales desafíos para el turismo es el tratamiento y la prevención de envenenamientos por medusas. Hasta el momento se conocen tres especies de medusas urticantes que arriban a la costa: Chrysaora lactea, Liriope tetraphylla y Olindias sambaquiensis. Los objetivos de este trabajo fueron: calcular los parámetros poblacionales de las medusas urticantes, efectuar el diagnóstico diferencial de los síntomas causados por envenenamiento, analizar el número de afectados en relación a la abundancia de medusas urticantes, evaluar la elección diferencial del centro de atención primaria elegido por los afectados y confeccionar un algoritmo diagnóstico. El número de medusas urticantes en el año 2016 fue cinco veces mayor que en 2017. Liriope tetraphylla registró las mayores abundancias promedio, frecuencias y dominancias relativas. En 2016 coincidió su mayor abundancia con el mayor porcentaje de afectados. En 2016 se registraron 69 casos totales de envenenamiento y en 2017 se identificaron 79 casos. En 2017 el porcentaje de personas afectadas por O. sambaquiensis no presentó una correlación con la abundancia promedio de esta especie. Liriope tetraphylla causó lesiones eritematomaculo-papulares acompañadas de prurito sin dolor, C. lactea provocó lesiones eritemato-vesiculosas de dolor moderado y O. sambaquiensis lesiones eritemato-edematosas lineales de dolor intenso con hiperpigmentación residual. No existe tratamiento específico para el envenenamiento, la terapéutica diferencial es sintomática. Las primeras acciones son comunes para las tres especies: lavar con solución fisiológica o agua de mar limpia y aplicar compresas frías.

On the Southwest coast of Buenos Aires Province one of the main challenges for tourism is the treatment and prevention of poisoning by jellyfish. So far, three species of venomous jellyfish arriving to the coast have been identified: Chrysaora lactea, Liriope tetraphylla, and Olindias sambaquiensis. The objectives of this study were: to calculate the population parameters of the venomous jellyfish, to carry out a differential diagnosis of the symptoms caused by poisoning, to analyze the number of individuals affected in relation to the abundance of this jellyfish, to evaluate which primary care centers are chosen by those affected, and to develop a diagnostic algorithm. The number of venomous jellyfish in 2016 was five times higher than in 2017. The highest average abundance, frequency, and relative dominance were recorded for L. tetraphylla. In 2016, its greatest abundance coincided with the highest percentage of people affected by this species. A total of 69 confirmed cases of poisoning caused by the three species were recorded in 2016 and 79 cases in 2017. In 2017, the percentage of people affected by O. sambaquiensis was not correlated with the average abundance of this species. Liriope tetraphylla caused erythematous-maculopapular lesions accompanied by painless pruritus; C. lactea caused erythematous-vesicular lesions of moderate pain and O. sambaquiensis caused linear erythematous-edematous lesions with severe pain and residual hyperpigmentation. At present, there is no specific treatment for poisoning, the differential therapy is symptomatic. The recommended actions are common for all three species: wash the skin with physiological solution or clean sea water and apply cold compresses.
Descritores: Venenos de Cnidários
-Envenenamento
FUNERAL RITESABDOMINAL INJURIES
Limites: Humanos
Responsável: AR393.1 - Centro de Información y Documentación Dr H. Urquiola


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Id: lil-618190
Autor: Thangaraj, S; Bragadeeswaran, S.
Título: Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India
Fonte: J. venom. anim. toxins incl. trop. dis;18(1):53-61, 2012. ilus.
Idioma: en.
Resumo: Cnidarians comprise an old and diverse animal phylum, and possess a wide variety of biologically active substances. Sea anemones contain a diversity of interesting biologically active compounds including some potent toxins. In the present work, the sea anemones Stichodactyla mertensii and Stichodactyla gigantea, collected from the Mandapam coast, are characterized biomedically and pharmacologically. The crude protein was obtained by using methanol and aqueous extracts. The respective protein contents of S. mertensii and S. gigantea were found to be 2.10 µg/mL and 1.87 µg/mL. The methanol and aqueous extracts of S. mertensii and S. gigantea yielded six and nine bands by SDS-PAGE on 12 percent gel. In the hemolytic assay, both extracts exhibited hemolytic effect on chicken, goat, cow and human erythrocytes ('A', 'B' and 'O'). The neurotoxic effects of these crude extracts were determined in vivo using the sea shore crab Ocypode macrocera and mortality was observed. The mouse bioassay for lethality was performed on male albino mice. The crude extract of S. mertensii showed higher lethality (58 seconds at 1 mL-dose) than that of S. gigantea (2 minutes and 10 seconds at 0.75 mL-dose). The analgesic activity test was also carried out on albino mice by Eddy's hot plate and tail-flick methods. The extracts showed moderate analgesic effect by both hot-plate and tail-flick methods. These characteristics emphasize the need for the isolation and molecular characterization of new active toxins in S. mertensii and S. gigantea.(AU)
Descritores: Anêmonas-do-Mar/química
Antivenenos
Venenos de Cnidários/toxicidade
Neurotoxinas/química
-Bioensaio/métodos
Proteínas Hemolisinas/isolamento & purificação
Proteínas Hemolisinas/efeitos dos fármacos
Analgésicos/farmacologia
Limites: Animais
Masculino
Ratos
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: biblio-949792
Autor: González, Claudia Analía; Bertrand, Roxana Andrea.
Título: Olindias sambaquiensis en la costa Atlántica Argentina / Olindias sambaquiensis on the Atlantic coast of Argentina
Fonte: Acta toxicol. argent;25(2):52-57, set. 2017. ilus.
Idioma: es.
Descritores: Venenos de Cnidários
Cifozoários
-Argentina/epidemiologia
Ácido Acético/uso terapêutico
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: lil-741532
Autor: İsmi, Onur; Görür, Kemal; Arpacı, Rabia Bozdoğan; Vayisoglu, Yusuf; Özcan, Cengiz.
Título: Hairy Polyp of the Supratonsillar Fossa Causing Intermittent Airway Obstruction
Fonte: Int. arch. otorhinolaryngol. (Impr.);19(1):90-92, Jan-Mar/2015. graf.
Idioma: en.
Resumo: Introduction Dermoids frequently called "hairy polyps" and their nature have not been completely clarified. Objectives To discuss the unusual presentation, symptoms, incidence, histology, and perioperative management of hairy polyps in the light of a case and current literature. Resumed Report A 3-year-old boy presented with intermittent respiratory distress since birth. Oropharyngeal examination revealed a nasopharyngeal mass originating from the supratonsillar fossa. The mass was so mobile that it moved between the oropharynx and the nasopharynx during swallowing. The radiologic and pathologic examinations confirmed the mass as a hairy polyp. Conclusion In a pediatric age group with airway obstruction, hairy polyps of the oropharyngeal region must also be included in the differential diagnosis. .
Descritores: Venenos de Cnidários/química
Bicamadas Lipídicas/química
Gotículas Lipídicas/química
Imagem Molecular/métodos
-Galinhas
Porosidade
Ovinos
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-724673
Autor: Haddad Junior, Vidal; Zara, Fernando; Marangoni, Sergio; Toyama, Daniela de Oliveira; Souza, Alex Jardelino Felizardo de; Oliveira, Simone Cristina Buzzo de; Toyama, Marcos Hikari.
Título: Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria)
Fonte: J. venom. anim. toxins incl. trop. dis;20, 04/02/2014.
Idioma: en.
Resumo: Although the hydrozoan Olindias sambaquiensis is the most common jellyfish associated with human envenomation in southeastern and southern Brazil, information about the composition of its venom is rare. Thus, the present study aimed to analyze pharmacological aspects of O. sambaquiensis venom as well as clinical manifestations observed in affected patients. Crude protein extracts were prepared from the tentacles of animals; peptides and proteins were sequenced and submitted to circular dichroism spectroscopy. Creatine kinase, cytotoxicity and hemolytic activity were evaluated by specific methods.
Descritores: Anemia Hemolítica
Citotoxinas/análise
Envenenamento
Venenos de Cnidários/análise
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-691433
Autor: Haddad Junior, Vidal; Virga, Rossana; Bechara, Adriano; Silveira, Fabio Lang da; Morandini, Andre Carrara.
Título: An outbreak of Portuguese man-of-war (Physalia physalis - Linnaeus, 1758) envenoming in Southeastern Brazil
Fonte: Rev. Soc. Bras. Med. Trop;46(5):641-644, Sept-Oct/2013. graf.
Idioma: en.
Resumo: Introduction Portuguese man-of-war, Physalia physalis (Linnaeus, 1758), are cnidarians capable of discharging intracellular organelles filled with venom, resulting in severe envenomation in humans. Methods We report the clinical and therapeutic aspects of 331 accidents involving Portuguese man-of-war in an outbreak on the coast of the State of São Paulo, Brazil. Results The clinical manifestations of envenomation were rare and mild and mostly local, systemic reactions; there was a low rate of late complications. Conclusions The consequences of envenomation were of moderate severity, and first aid measures were effective in controlling the pain. Outbreaks of accidents involving Portuguese man-of-war occur periodically in the area without a clear explanation. .
Descritores: Mordeduras e Picadas/epidemiologia
Venenos de Cnidários/envenenamento
Surtos de Doenças
Hidrozoários
Dermatopatias Vesiculobolhosas/epidemiologia
-Mordeduras e Picadas/etiologia
Brasil/epidemiologia
Dermatopatias Vesiculobolhosas/etiologia
Limites: Adolescente
Adulto
Animais
Criança
Pré-Escolar
Feminino
Humanos
Lactente
Masculino
Pessoa de Meia-Idade
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-686622
Autor: Monroy-Estrada, Heidi I; Chirino, Yolanda I; Soria-Mercado, Irma E; Sanchez-Rodriguez, Judith.
Título: Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells
Fonte: J. venom. anim. toxins incl. trop. dis;19:12-12, maio 2013.
Idioma: en.
Resumo: Background: Lung cancer causes 1.4 million deaths worldwide while non-small-cell lung cancer (NSCLC) represents 80-85% of the cases. Cisplatin is a standard chemotherapy against this type of cancer; however, tumor cell resistance to this drug limits its efficacy. Sea anemones produce compounds with pharmacological activities that may be useful for augmenting cisplatin efficacy. This study aimed to evaluate the pharmacological activities of crude venom (CV) from the sea anemone Bunodeopsis globulifera and four derived fractions (F1, F2, F3 and F4) to test their increase efficiency cisplatin cytotoxicity in human lung adenocarcinoma cells. Results: Pre-exposure to CV, F1 and F2 fractions increases cisplatin cytotoxicity in human lung adenocarcinoma cells under specific conditions. Exposure to CV at 50 μgmL-1 induced a reduction of approximately 50% in cell viability, while a similar cytotoxic effect was observed when cell culture was exposed to F1 at 25 μgmL -1 or F2 at 50 μgmL-1. The cell culture exposure to F1 (10 μgmL-1) fraction combined with cisplatine (25 μM) provoked a decrease in MTT reduction until 65.57% while F2 (25 μgmL-1) fraction combined with cisplatin (10 μM) provoked a decrease in MTT reduction of 72.55%. Conclusions: The F1 fraction had the greatest effect on the lung adenocarcinoma cell line compared with CV and F2. The combination of antineoplastic drugs and sea anemone toxins might allow a reduction of chemotherapeutic doses and thus mitigate side effects.
Descritores: Neoplasias Pulmonares
Venenos de Cnidários/farmacologia
Venenos de Cnidários/uso terapêutico
Limites: Humanos
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação



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