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Pesquisa : D20.888.230 [Categoria DeCS]
Referências encontradas : 45 [refinar]
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Id: lil-542433
Autor: Karthikayalu, Subbrayalu; Rama, V; Kirubagaran, R; Venkatesan, R.
Título: Hemolytic toxin from the soft coral Sarcophyton trocheliophorum: isolation and physiological characterization
Fonte: J. venom. anim. toxins incl. trop. dis;16(1):107-120, 2010. ilus, graf, tab.
Idioma: en.
Resumo: The unifying characteristic of cnidarians is the production of protein and polypeptide toxins. The present study describes the identification of a hemolytic toxin from the soft coral Sarcophyton trocheliophorum. The crude extract was highly cytotoxic (EC50 = 50 ng/mL) against human erythrocytes. It was also tested for hemolytic activity by the blood agar plate method, resulting in a hemolytic halo of 12 mm with 50 µg of protein. The stability of the venom under different physiological conditions was analyzed. The venom hemolytic activity was augmented by alkaline and neutral pH whereas it was reduced in acidic pH. The activity was stable up to 60º C. The hemolytic activity was completely abolished by the addition of serum and reduced significantly during frequent freezing-thawing cycles. Toxin purification was performed by ammonium sulfate precipitation and subsequently desalted by dialysis against 10 mM sodium phosphate buffer (pH 7.2), followed by anion exchange chromatography on DEAE cellulose column and gel filtration chromatography using Sephadex G-50 matrix. The purified active fractions possessed a prominent protein of approximately 45 kDa, as revealed by SDS-PAGE.(AU)
Descritores: Cnidários/fisiologia
Venenos de Cnidários/toxicidade
Diálise
Eritrócitos
-Proteínas
Cromatografia em Gel
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-639474
Autor: Ramezanpour, M; Burke da Silva, K; Sanderson, B. J.
Título: Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms
Fonte: J. venom. anim. toxins incl. trop. dis;18(2):157-163, 2012. graf.
Idioma: en.
Resumo: Although sea anemones are well known for being rich sources of toxins, including cytolysins and neurotoxins, their venoms and toxins have been poorly studied. In the present study, the venoms from five sea anemones (Heteractis crispa, Heteractis magnifica, Heteractis malu, Cryptodendrum adhaesivum and Entacmaea quadricolor) were obtained by the milking technique, and the potential of these venoms to kill cancer cells was tested on three cell lines (A549 lung cancer, T47D breast cancer and A431 skin cancer). The total protein level in the crude extract was determined by the bicinchoninic acid (BCA) protein assay. The cytotoxicity on different cell lines was assayed using the 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide (MTT) assay which measures survival based on the detection of mitochondrial activity and by the crystal violet assay, which measures survival based on the ability of cells to remain adherent to microplates. The results indicate that the sea anemone venom is cytotoxic to human cancer cells. The A549 cell line was the most sensitive of the cell lines tested with a significant reduction in viability observed at 40 µg/mL. H. malu, C. adhaesivum and E. quadricolor had a significant inhibitory effect on A431 cells. Furthermore, H. malu and C. adhaesivum had a significant inhibitory effect on T47D cell line at 40 µg/mL. In conclusion, the sea anemone venoms tested have the potential to be developed as anticancer agents.(AU)
Descritores: Anêmonas-do-Mar
Neoplasias Cutâneas
Neoplasias da Mama
Anticarcinógenos/análise
Venenos de Cnidários
Neoplasias Pulmonares
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-954713
Autor: García-Arredondo, Alejandro; Murillo-Esquivel, Luis J; Rojas, Alejandra; Sanchez-Rodriguez, Judith.
Título: Characteristics of hemolytic activity induced by the aqueous extract of the Mexican fire coral Millepora complanata
Fonte: J. venom. anim. toxins incl. trop. dis;20:49, 04/02/2014. tab, ilus, graf.
Idioma: en.
Projeto: UAQ; . UAQ; . UAQ.
Resumo: Background Millepora complanata is a plate-like fire coral common throughout the Caribbean. Contact with this species usually provokes burning pain, erythema and urticariform lesions. Our previous study suggested that the aqueous extract of M. complanata contains non-protein hemolysins that are soluble in water and ethanol. In general, the local damage induced by cnidarian venoms has been associated with hemolysins. The characterization of the effects of these components is important for the understanding of the defense mechanisms of fire corals. In addition, this information could lead to better care for victims of envenomation accidents.Methods An ethanolic extract from the lyophilized aqueous extract was prepared and its hemolytic activity was compared with the hemolysis induced by the denatured aqueous extract. Based on the finding that ethanol failed to induce nematocyst discharge, ethanolic extracts were prepared from artificially bleached and normal M. complanata fragments and their hemolytic activity was tested in order to obtain information about the source of the heat-stable hemolysins.Results Rodent erythrocytes were more susceptible to the aqueous extract than chicken and human erythrocytes. Hemolytic activity started at ten minutes of incubation and was relatively stable within the range of 28-50°C. When the aqueous extract was preincubated at temperatures over 60°C, hemolytic activity was significantly reduced. The denatured extract induced a slow hemolytic activity (HU50= 1,050.00 ± 45.85 μg/mL), detectable four hours after incubation, which was similar to that induced by the ethanolic extract prepared from the aqueous extract (HU50= 1,167.00 ± 54.95 μg/mL). No significant differences were observed between hemolysis induced by ethanolic extracts from bleached and normal fragments, although both activities were more potent than hemolysis induced by the denatured extract.Conclusions The results showed that the aqueous extract of M. complanata possesses one or more powerful heat-labile hemolytic proteins that are slightly more resistant to temperature than jellyfish venoms. This extract also contains slow thermostable hemolysins highly soluble in ethanol that are probably derived from the body tissues of the hydrozoan.(AU)
Descritores: Venenos de Cnidários
Hidrozoários
Mecanismos de Defesa
Hemólise
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-954832
Autor: Bae, Seong Kyeong; Lee, Hyunkyoung; Heo, Yunwi; Pyo, Min Jung; Choudhary, Indu; Han, Chang Hoon; Yoon, Won Duk; Kang, Changkeun; Kim, Euikyung.
Título: In vitro characterization of jellyfish venom fibrin(ogen)olytic enzymes from Nemopilema nomurai
Fonte: J. venom. anim. toxins incl. trop. dis;23:35, 2017. graf, ilus.
Idioma: en.
Projeto: National Institute of Fisheries Science; . Korea government.
Resumo: Background: Because jellyfish are capable of provoking envenomation in humans, they are considered hazardous organisms. Although the effects of their toxins are a matter of concern, information on the venom components, biological activity and pathological mechanisms are still scarce. Therefore, the aim of the present study was to investigate a serine protease component of Nemopilema nomurai jellyfish venom (NnV) and unveil its characteristics. Methods: To determine the relationship between fibrinolytic activity of NnV and the serine protease, fibrin zymography was performed using metalloprotease and serine protease inhibitors. The biochemical characterization of serine proteases of NnV were determined by the amidolytic assay. Fractions with fibrinolytic activity were obtained by DEAE cation exchange column. Results: NnV displayed fibrinolytic activities with molecular masses of approximately 70, 35, 30, and 28 kDa. The fibrinolytic activity of NnV was completely obliterated by phenylmethylsulfonyl fluoride, a prototype serine protease inhibitor. Based on amidolytic assays using chromogenic substrates specific for various kinds of serine proteases, NnV predominantly manifested a chymotrypsin-like feature. Its activity was completely eliminated at low pH (< 6) and high temperatures (> 37 °C). Some metal ions (Co2+, Cu2+, Zn2+ and Ni2+) strongly suppressed its fibrinolytic activity, while others (Ca2+ and Mg2+) failed to do so. Isolation of a serine protease with fibrionolytic activity from NnV revealed that only p3 showed the fibrinolytic activity, which was completely inhibited by PMSF. Conclusion: The present study showed that N. nomurai jellyfish venom has a chymotrypsin-like serine protease with fibrinolytic activity. Such information might be useful for developing clinical management of jellyfish envenomation and pharmacological agents with therapeutic potential for thrombotic diseases in the future.(AU)
Descritores: Fluoreto de Fenilmetilsulfonil
Técnicas In Vitro
Fibrina
Quimotripsina
Venenos de Cnidários
Metaloproteases
Enzimas
Serina Proteases
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-1278223
Autor: Genzano, Gabriel; Puente Tapia, Francisco Alejandro; Dutto, Sofía; Schiariti, Agustín.
Título: Las medusas en los balnearios de la provincia de Buenos Aires / The jellyfish species in the coastal waters of Buenos Aires province
Fonte: Acta toxicol. argent;28(2):53-59, Sept. 2020. graf.
Idioma: es.
Resumo: Resumen Las medusas son organismos mayoritariamente marinos pertenecientes al grupo de los cnidarios, los cuales se caracterizan por presentar células urticantes especializadas, los cnidocitos. Si bien, todas las medusas son potencialmente venenosas y el grado de toxicidad depende de la especie, sus efectos sobre los humanos varían desde reacciones locales leves hasta reacciones atópicas-anafilácticas graves, llegando incluso a la muerte de la víctima. Mundialmente se conocen numerosas especies de medusas causantes de envenenamiento a humanos, tales como la avispa de mar (Chironex fleckeri), el sifonóforo carabela portuguesa (Physalia physalis) o el hidrocoral de fuego (Millepora spp.). En Argentina, tres especies de medusas revisten de im portancia clínica epidemiológica debido a su poder urticante: las hidromedusas Liriope tetraphylla y Olindias sambaquiensis, así como la escifomedusa Chrysaora lactea. Estas especies presentan sus mayores abundancias en el verano en las costas bonae renses, coincidiendo con la presencia de turistas durante el periodo vacacional. Sus afectaciones varían de leves a moderadas, registrándose desde parestesias y ardor con dermatitis, prurito, edemas y eritemas. Una infinidad de "remedios caseros" se conocen para remediar los efectos de las picaduras de medusas, sin embargo, la mayoría han resultado ineficaces y perjudiciales. Lo más recomendable es evitar frotar y lavar la zona afectada con agua dulce o aplicar hielo para tratar de contrarrestar el ardor y acudir lo antes posible al centro de salud más cercano.

Abstract Medusae are mainly a marine group belonged to cnidarians, which are characterized by specialized stinging cells, cnidocyts. Although all medusae are potentially poisonous, their toxicity depends on the species and the effects on humans vary- ing from mild local reactions to severe atopic-anaphylactic reactions, even the death of the victim. Numerous species of cnidar ians are known worldwide to affect humans, such as the sea wasp {Chironex fleckeri), the siphonophore portuguese man-of-war (Physaliaphysalis) or the fire-coral (Millepora spp.). In Argentina, three species of medusae are known with clinical epidemiological importance due to their stinging power: the hydromedusae Liriopetetraphylla and Olindias sambaquiensis, as well as the scypho- medusae Chrysaora lactea. These species have their highest abundances in the summer on Buenos Aires coasts, coinciding with the presence of tourists during the summer vacations. Its affectations vary from mild to moderate, registering from paresthesias and burning with dermatitis, itching, edemas, and erythema. An infinity of "home remedies" are known formedusae stings, how- ever, most of them have proven ineffective and harmful. It is best to avoid rubbing and washing the affected area with fresh water or applying ice to try to counteract the burning in the region and to go the health center as soon as possible.
Descritores: Cnidários/patogenicidade
Venenos de Cnidários/toxicidade
-Argentina
Nematocisto/lesões
Responsável: AR658.1 - Biblioteca Central "Leopoldo Marechal"


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Id: biblio-1041394
Autor: Bastos, Denise Maria Ramalho Ferreira; Haddad Júnior, Vidal; Nunes, Jorge Luiz Silva.
Título: Human envenomations caused by Portuguese man-of-war (Physalia physalis) in urban beaches of São Luis City, Maranhão State, Northeast Coast of Brazil
Fonte: Rev. Soc. Bras. Med. Trop;50(1):130-134, Jan.-Feb. 2017. graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa e Desenvolvimento Científico do Maranhão (FAPEMA).
Resumo: ABSTRACT INTRODUCTION: The clinical and epidemiological aspects associated with Portuguese man-of-war envenomation were investigated and characterized. METHODS: Data from recorded envenomation events between 2005 and 2013 were provided by the GBMar (Group of Firemen Maritime of Maranhão State) and SEMUSC (Municipal Secretary of Security with Citizenship). RESULTS: Most victims were children, and clinical manifestations included intense pain, edema, erythema, and rare systemic manifestations. CONCLUSIONS: The envenomation events were predictable and based on patterns involving multiple factors (environmental and/or human behavior); however, the initially applied measures did not match the current recommendations of the Health Ministry of Brazil.
Descritores: Venenos de Cnidários/envenenamento
Hidrozoários
-Estações do Ano
Praias
Brasil/epidemiologia
Pessoa de Meia-Idade
Limites: Humanos
Animais
Masculino
Feminino
Lactente
Pré-Escolar
Criança
Adolescente
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-897045
Autor: Pereira, João Claudio Campos; Szpilman, David; Haddad Junior, Vidal.
Título: Anaphylactic reaction/angioedema associated with jellyfish sting
Fonte: Rev. Soc. Bras. Med. Trop;51(1):115-117, Jan.-Feb. 2018. graf.
Idioma: en.
Resumo: Abstract The most frequent jellyfish in Southern Brazil causes mainly local pain and skin plaques. A 3-year-old female bather presented an erythematous, irregular plaque on the left forearm after contact with a jellyfish and intense facial angioedema with facial flushing. The lungs had vesicular murmur, wheezes, and snorts, and pink and spumous secretion in the airways with intercostal retraction. She was administered subcutaneous adrenaline (0.1mg/kg) and hydrocortisone intravenous (10mg/kg) with total recovery in a few minutes. The manifestations of anaphylactic reactions are distinct from those of envenomations, and prompt and adequate care is fundamental in these situations.
Descritores: Mordeduras e Picadas/complicações
Cnidários/classificação
Venenos de Cnidários
Anafilaxia/etiologia
Angioedema/etiologia
-Anafilaxia/diagnóstico
Angioedema/diagnóstico
Limites: Humanos
Animais
Feminino
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: biblio-1041566
Autor: Haddad Junior, Vidal; Costa, Maurício Azevedo de Oliveira; Nagata, Renato.
Título: Outbreak of jellyfish envenomations caused by the species olindias sambaquiensis (cnidaria: hydrozoa) in the Rio Grande do Sul state (Brazil)
Fonte: Rev. Soc. Bras. Med. Trop;52:e20190137, 2019. graf.
Idioma: en.
Descritores: Mordeduras e Picadas/epidemiologia
Surtos de Doenças
Venenos de Cnidários/envenenamento
Hidrozoários
-Estações do Ano
Índice de Gravidade de Doença
Brasil/epidemiologia
Limites: Animais
Tipo de Publ: Carta
Responsável: BR1.1 - BIREME


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Id: biblio-1087405
Autor: Young, Pablo; Rey, Graciela.
Título: Manifestaciones clínicas de las picaduras por medusa en la costa argentina / Clinical manifestations of jellyfish stings on the Argentine coast
Fonte: Salud(i)ciencia (Impresa) = Salud(i)ciencia (En linea);22(5):450-453, mayo-jun. 2017.
Idioma: es.
Descritores: Envenenamento
Mordeduras e Picadas
Venenos de Cnidários
Anafilaxia
Cifozoários
Tipo de Publ: Entrevista
Responsável: AR392.1 - Biblioteca


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Id: lil-317782
Autor: Nonato, Edmundo Ferraz; Málaque, Célia Maria Sant'Ana; Haddad Júnior, Vidal.
Título: Acidentes por animais aquáticos / Accidents for aquatic animals
Fonte: In: Veronesi, Ricardo; Focaccia, Roberto. Tratado de infectologia: v.2. Säo Paulo, Atheneu, 2002. p.1563-1569, ilus. (BR)
Idioma: pt.
Símbolo: BR.
Descritores: Acidentes
Fauna Aquática
Peçonhas
-Peixes Venenosos
Venenos de Cnidários
Equinodermos
Peixes
Anelídeos
Venenos de Moluscos
Poríferos
Responsável: BR31.1 - SIDC - Serviço de Informação e Documentação Científica
BR31.1; WC100, V599t, 2.ed., v.2



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