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Pesquisa : D20.888.590 [Categoria DeCS]
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Texto completo SciELO Brasil
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Id: biblio-1375812
Autor: Fu, Ying; Zhang, Yu; Ju, Shuang; Ma, Bokai; Huang, Wenwen; Luo, Sulan.
Título: Isolation and characterization of five novel disulfide-poor conopeptides from Conus marmoreus venom
Fonte: J. venom. anim. toxins incl. trop. dis;28:e20210116, 2022. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Background: Conopeptides from cone snail venom have aroused great interest related to the discovery of novel bioactive candidates, due to their excellent prospects for the treatment of various health problems such as pain, addiction, psychosis and epilepsy. In order to explore novel biopeptides, we investigated the structure and function of five novel conopeptides isolated from the venom of Conus marmoreus from South China Sea. Methods: C. marmoreus crude venom was prepared, fractionated and purified by HPLC system. The primary sequences of the five novel disulfide-poor conopeptides Mr-1 to Mr-5 were identified by comprehensive analysis of de novo MALDI-TOF tandem mass spectrometry and Edman degradation data. In order to investigate their function, these five conopeptides were synthesized by Fmoc-SPPS chemistry, and their biological effects at several heterologous rat nicotinic acetylcholine receptor (nAChR) subtypes (α1β1δε, α3β2, α3β4, α4β2) were determined by electrophysiological technique. Results: Five novel disulfide-poor conopeptides were identified and named as follows: Mr-1 (DWEYHAHPKPNSFWT), Mr-2 (YPTRAYPSNKFG), Mr-3 (NVIQAPAQSVAPP NTST), Mr-4 [KENVLNKLKSK(L/I)] and Mr-5 [NAVAAAN(L/I)PG(L/I)V]. None of them contains a disulfide bond. The sequences of conopeptides Mr-2 to Mr-5 do not belong to any category of the known disulfide-poor conopeptides. No significant activity against the above nAChR subtypes were observed for the five conopeptides at 100 µM. Conclusion: We purified and structurally characterized five novel disulfide-poor conopeptides from C. marmoreus crude venom and first investigated their nAChR inhibitory effects. This work expanded our knowledge on the structure and function of disulfide-poor conopeptides from this cone snail venom.(AU)
Descritores: Conotoxinas/isolamento & purificação
Dissulfetos/efeitos adversos
Venenos de Moluscos
-Espectrometria de Massas
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Texto completo SciELO Costa Rica
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Id: biblio-843314
Autor: Quijano-Scheggia, Sonia; Barajas-Gonzalez, Maribel; Chang-Lim, Hong; Pi-Leaw, Chui; Olivos-Ortiz, Aramis; Gaviño-Rodriguez, Juan; Blanco-Pérez, Juan; S-Bates, Stephen.
Título: The inhibitory effect of a non-yessotoxin-producing dinoflagellate, Lingulodinium polyedrum (Stein) Dodge, towards Vibrio vulnificus and Staphylococcus aureus
Fonte: Rev. biol. trop;64(2):805-816, abr.-jun. 2016. tab, ilus.
Idioma: es.
Resumo: ResumenEl aumento de la resistencia bacteriana a los antibióticos ha causado preocupación a nivel mundial, por lo que se ha promovido la búsqueda de nuevos compuestos. Debido a su abundancia y diversidad, el fitoplancton marino constituye una importante fuente potencial de tales compuestos. La investigación sobre dinoflagelados ha llevado al descubrimiento de inhibidores de crecimiento bacteriano. El dinoflagelado marino Lingulodinium polyedrum causa proliferaciones algales en diferentes regiones del mundo, incluyendo México, y también se sabe que regula el crecimiento de otras especies en las aguas costeras. En este trabajo, se investiga la taxonomía de este dinoflagelado y se caracteriza la capacidad de sus extractos para inhibir el crecimiento de dos bacterias de importancia médica (Vibrio vulnificus y Staphylococcus aureus) en placas de cultivo de agar. La caracterización taxonómica se realizó por PCR y amplificación del gen de ITS, y se confirmó que la especie aislada en la costa del Pacífico de México fue L. polyedrum. Para demostrar el efecto inhibidor de los extractos de L. polyedrum, los cultivos se cosecharon por centrifugación. Los pellets de tres abundancias celulares se extrajeron con agua, metanol, hexano y cloroformo. Los experimentos en V. vulnificus mostraron una inhibición alta del crecimiento para los cuatro extractos, variando entre 77 y 98 %. Sorprendentemente, la inhibición del crecimiento fue menor cuando los extractos se originaron a partir de una mayor abundancia de células L. polyedrum, varía de 0 a 34 %. Para S. aureus, la inhibición del crecimiento también fue alta, pero no estadísticamente diferente para todos los extractos y abundancias de células, con un rango de 62 hasta 99 %. Esto resultados son prometedores para futuras aplicaciones farmacológicas. La cepa mexicana de L. polyedrum no produjo yesotoxinas detectables.

AbstractThe increased bacterial resistance to antibiotics has caused global concern, prompting the search for new compounds. Because of their abundance and diversity, marine phytoplankton are an important potential source of such compounds. Research on dinoflagellates has led to the discovery of inhibitors of bacterial growth. The marine dinoflagellate Lingulodinium polyedrum blooms in different regions of the world, including Mexico, and is also known to regulate the growth of other species in coastal waters. Here, we investigated the taxonomy of this dinoflagellate and characterized the ability of its extracts to inhibit the growth of two bacteria of medical importance (Vibrio vulnificus and Staphylococcus aureus). Taxonomic characterization was performed by PCR and gene amplification of ITS, and confirmed that the species isolated off the Pacific coast of Mexico was L. polyedrum. To prove the inhibitory effect of L. polyedrum extracts, cultures were harvested by centrifugation. Pellets from three cellular abundances were extracted with water, methanol, hexane and chloroform. The experiments on V. vulnificus showed a high growth inhibition for the four extracts, ranging from 77 to 98 %. Surprisingly, the growth inhibition was lower when the extracts originated from a higher L. polyedrum cell abundance, ranging from 0 to 34 %. For S. aureus, the growth inhibition was also high, but not statistically different for all extracts and cell abundances, ranging from 62 to 99 %. This study obtained promising results for future pharmacological applications. Our Mexican strain of L. polyedrum did not produce any detectable yessotoxins. Rev. Biol. Trop. 64 (2): 805-816. Epub 2016 June 01.
Descritores: Staphylococcus aureus/efeitos dos fármacos
Dinoflagelados/química
Vibrio vulnificus/efeitos dos fármacos
Oxocinas/farmacologia
-Dinoflagelados/genética
Reação em Cadeia da Polimerase
Venenos de Moluscos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: biblio-954780
Autor: Lebbe, Eline K. M; Tytgat, Jan.
Título: In the picture: disulfide-poor conopeptides, a class of pharmacologically interesting compounds
Fonte: J. venom. anim. toxins incl. trop. dis;22:30, 2016. tab, graf, ilus.
Idioma: en.
Projeto: F.W.O. Vlaanderen; . Inter-University Attraction Poles Program, Belgian State, Belgian Science Policy; . KU Leuven.
Resumo: During evolution, nature has embraced different strategies for species to survive. One strategy, applied by predators as diverse as snakes, scorpions, sea anemones and cone snails, is using venom to immobilize or kill a prey. This venom offers a unique and extensive source of chemical diversity as it is driven by the evolutionary pressure to improve prey capture and/or to protect their species. Cone snail venom is an example of the remarkable diversity in pharmacologically active small peptides that venoms can consist of. These venom peptides, called conopeptides, are classified into two main groups based on the number of cysteine residues, namely disulfide-rich and disulfide-poor conopeptides. Since disulfide-poor conotoxins are minor components of this venom cocktail, the number of identified peptides and the characterization of these peptides is far outclassed by its cysteine-rich equivalents. This review provides an overview of 12 families of disulfide-poor peptides identified to date as well as the state of affairs.(AU)
Descritores: Peptídeos
Caramujos
Conotoxinas
Venenos de Moluscos
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Texto completo SciELO Brasil
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Id: biblio-1346437
Autor: Fouda, Maged M. A; Abdel-Wahab, Mohammed; Mohammadien, Amal; Germoush, Mousa O; Sarhan, Moustafa.
Título: Proteomic analysis of Red Sea Conus taeniatus venom reveals potential biological applications
Fonte: J. venom. anim. toxins incl. trop. dis;27:e20210023, 2021. tab, graf, ilus, mapas.
Idioma: en.
Projeto: Jouf University.
Resumo: Diverse and unique bioactive neurotoxins known as conopeptides or conotoxins are produced by venomous marine cone snails. Currently, these small and stable molecules are of great importance as research tools and platforms for discovering new drugs and therapeutics. Therefore, the characterization of Conus venom is of great significance, especially for poorly studied species. Methods: In this study, we used bioanalytical techniques to determine the venom profile and emphasize the functional composition of conopeptides in Conus taeniatus, a neglected worm-hunting cone snail. Results: The proteomic analysis revealed that 84.0% of the venom proteins were between 500 and 4,000 Da, and 16.0% were > 4,000 Da. In C. taeniatus venom, 234 peptide fragments were identified and classified as conotoxin precursors or non-conotoxin proteins. In this process, 153 conotoxin precursors were identified and matched to 23 conotoxin precursors and hormone superfamilies. Notably, the four conotoxin superfamilies T (22.87%), O1 (17.65%), M (13.1%) and O2 (9.8%) were the most abundant peptides in C. taeniatus venom, accounting for 63.40% of the total conotoxin diversity. On the other hand, 48 non-conotoxin proteins were identified in the venom of C. taeniatus. Moreover, several possibly biologically active peptide matches were identified, and putative applications of the peptides were assigned. Conclusion: Our study showed that the composition of the C. taeniatus-derived proteome is comparable to that of other Conus species and contains an effective mix of toxins, ionic channel inhibitors and antimicrobials. Additionally, it provides a guidepost for identifying novel conopeptides from the venom of C. taeniatus and discovering conopeptides of potential pharmaceutical importance.(AU)
Descritores: Proteoma
Conotoxinas
Caramujo Conus
Venenos de Moluscos
Neurotoxinas
-Produtos Biológicos
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Texto completo SciELO Chile
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Id: lil-676126
Autor: Santibañez Aguascalientes, Norma Angélica; Ortíz Ordóñez, Esperanza; Falcón Alcántara, Andrés; Heimer de la Cotera, Edgar P.
Título: Estudio histológico del tubo digestivo y aparato venenoso de Gemmula periscelida (Gastropoda: Turridae) / Histologic study of digestive tract and venom apparatus of Gemmula periscelida (Gastropoda: Turridae)
Fonte: Int. j. morphol;31(1):7-14, mar. 2013. ilus.
Idioma: es.
Resumo: En el presente trabajo se describe anatómica e histológicamente el tubo digestivo y aparato venenoso de Gemmula periscelida (Gastropoda: Turridae) en ejemplares colectados al Noroeste de la Plataforma Continental Yucateca. Se determinó que el tipo de epitelio que reviste a cada una de las zonas del tubo digestivo (probóscide, esófago anterior, medio y posterior, estómago, glándula digestiva e intestino) y al aparato venenoso, es diferente a lo reportado para otros túrridos; por lo que se infiere el posible mecanismo de alimentación para esta especie.

In this paper we realized anatomical and histologically description of the digestive tract and venom apparatus of Gemmula periscelida (Gastropoda: Turridae) specimens collected northwest of the Yucatan Shelf. Results of analysis show that there are differences in the type of epithelium coating each of the areas of the digestive tract (proboscis, anterior, middle and posterior esophagus, stomach, digestive gland and intestine) and of a venom apparatus with respect to that reported for other turrid snails. This suggests the possible feeding mechanism for this species.
Descritores: Caramujos/anatomia & histologia
Trato Gastrointestinal/anatomia & histologia
-Peçonhas
Venenos de Moluscos
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Cuba
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Id: biblio-1126598
Autor: Hernández Ferreras, Kiria; Castillo Vila, Julio Alberto; Varona Green, Cecilia Margarita.
Título: Emergencia de Angiostrongylus cantonensis en el caracol gigante africano / Emergence of Angiostrongylus cantonensis in the giant African snail
Fonte: Rev. cuba. invest. bioméd;39(2):e621, abr.-jun. 2020.
Idioma: es.
Descritores: Angiostrongylus cantonensis/patogenicidade
Venenos de Moluscos/efeitos adversos
Tipo de Publ: Carta
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: lil-317782
Autor: Nonato, Edmundo Ferraz; Málaque, Célia Maria Sant'Ana; Haddad Júnior, Vidal.
Título: Acidentes por animais aquáticos / Accidents for aquatic animals
Fonte: In: Veronesi, Ricardo; Focaccia, Roberto. Tratado de infectologia: v.2. Säo Paulo, Atheneu, 2002. p.1563-1569, ilus. (BR)
Idioma: pt.
Símbolo: BR.
Descritores: Acidentes
Fauna Aquática
Peçonhas
-Peixes Venenosos
Venenos de Cnidários
Equinodermos
Peixes
Anelídeos
Venenos de Moluscos
Poríferos
Responsável: BR31.1 - SIDC - Serviço de Informação e Documentação Científica
BR31.1; WC100, V599t, 2.ed., v.2


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Id: lil-497804
Autor: Tempone, André Gustavo.
Título: Cutaneous secretion from the giant African snail, Achatina fulica, as a source of Antileishmanial compounds
Fonte: Rev. Inst. Adolfo Lutz;66(1):73-77, jan.-abr. 2007. graf.
Idioma: en.
Resumo: Leishmaniasis is a severe protozoan parasite disease, causing clinical symptoms from a single cutaneo usulceration to a progressive and fatal disease. The treatment is based on toxic chemotherapeutic compounds, being pentavalent antimonials the first line drugs. Natural products from Brazilian fauna, especially animal venoms and cutaneous secretions, are a rich source of novel chemical molecules which could beused as drug prototypes for the development of new therapeutics. This study describes for the first time the effective anti leishmanial activity of crude cutaneous secretion from the giant African snail, Achatinafulica, and its mammalian cells citotoxi city and also its potential mode of action against Leishmania promastigotes. The crude secretion showed an Effective Concentration (EC50) of 98.37 µg/mL againstL.(L.) chagasi promastigotes. By means of enzymatic assays, a L-amino acid oxidase (L-AAO) activity was detected in crude secretion, and the hydrogen peroxide produced by this enzyme revealed to be one of the compounds responsible for the antileishmanial effect. The use of catalase for H2O2 scavenging in Leishmania cultures incubated with crude secretion abolished 54% of parasite death.

A Leishmaniose é uma doença parasitária grave que causa desde uma única ulceração cutânea até uma doença progressiva e fatal. O tratamento é baseado em agentes quimioterápicos tóxicos, sendo indicados como fármacos de primeira escolha os antimoniais pentavalentes. Os produtos naturais derivados da fauna brasileira, especialmente venenos e secreções cutâneas de anfíbios, são fontes ricas de novas moléculas químicas, as quais podem ser utilizadas como protótipos farmacêuticos no desenvolvimento de novos fármacos. No presente trabalho, é feita a descrição inédita sobre a efetiva atividade anti-Leishmania da secreção cutânea do caramujo gigante Africano, Achatina fulica, bem como a sua citotoxicidade em células de mamíferos e seu possível mecanismo de ação contra os promastig.
Descritores: Venenos de Moluscos
Leishmania
Secreções Corporais
Venenos
Responsável: BR91.2 - Centro de Documentação


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Texto completo SciELO Brasil
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Id: lil-686610
Autor: Abdel-Rahman, Mohamed A; Abdel-Nabi, Ismail M; El-Naggar, Mohamed S; Abbas, Osama A; Strong, Peter N.
Título: Conus vexillum venom induces oxidative stress in Ehrlich's ascites carcinoma cells: an insight into the mechanism of induction
Fonte: J. venom. anim. toxins incl. trop. dis;19:10-10, maio 2013. ilus, tab.
Idioma: en.
Resumo: Background: It is estimated that venoms of marine cone snails (genus Conus) contain more than 100,000 different small peptides with a wide range of pharmacological and biological actions. Some of these peptides were developed into potential therapeutic agents and as molecular tools to understand biological functions of nervous and cardiovascular systems. In this study we examined the cytotoxic and anticancer properties of the marine vermivorous cone snail Conus vexillum (collected from Hurgada and Sharm El-Shaikh, Red Sea, Egypt) and suggest the possible mechanisms involved. The in vitro cytotoxic effects of Conus venom were assessed against Ehrlich's ascites carcinoma (EAC) cells. Results: Conus venom treatment resulted in concentration-dependent cytotoxicity as indicated by a lactate dehydrogenase leakage assay. Apoptotic effects were measured in vivo by measuring levels of reactive oxygen species and oxidative defense agents in albino mice injected with EAC cells. Conus venom (1.25 mg/kg) induced a significant increase (p < 0.05) in several oxidative stress biomarkers (lipid peroxidation, protein carbonyl content and reactive nitrogen intermediates) of EAC cells after 3, 6, 9 and 12 hours of venom injection. Conus venom significantly reduced (p < 0.05) the activities of oxidative defense enzymes (catalase and superoxide dismutase) as well as the total antioxidant capacity of EAC cells, as evidenced by lowered levels of reduced glutathione. Conclusions: These results demonstrate the cytotoxic potential of C. vexillum venom by inducing oxidative stress mediated mechanisms in tumor cells and suggest that the venom contains novel molecules with potential anticancer activity.(AU)
Descritores: Carcinoma de Ehrlich
Estresse Oxidativo
Caramujo Conus/citologia
Venenos de Moluscos/toxicidade
Venenos de Moluscos/farmacologia
-Técnicas In Vitro
Apoptose/fisiologia
Egito
Antineoplásicos/farmacologia
Limites: Animais
Masculino
Camundongos
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: lil-533867
Autor: Pereañez J., Jaime A; Vargas., Leidy J.
Título: Neurotoxinas de invertebrados como alternativas terapéuticas y herramientas en investigación básica / Neurotoxins from invertebrates as alternative therapeutic agents and tools in basic research
Fonte: Vitae (Medellín);16(1):155-163, jan.-abr. 2009.
Idioma: es.
Resumo: Los venenos que producen los animales son una mezcla compleja de proteínas, péptidos, enzimas y trazas deelementos no proteicos tales como carbohidratos y sales, cuya finalidad es inmovilizar la presa y comenzar a digerirla. Las toxinas son sustancias aisladas de venenos, con una o varias acciones específicas sobre las víctimas. Entre estos compuestos, son numerosos los que tienen acción sobre receptores específicos ubicados en el sistema nervioso central y/o periférico, mientras que otros ejercen sus efectos actuando sobre otras proteínas. Desde el descubrimiento en 1971, del péptido que dio origen al Captopril, y teniendo en cuenta que muchas toxinas son útiles como herramientas para el estudio de procesos fisiológicos, se comenzó a mirar los venenos de animales como fuentes ricas en compuestos bioactivos y a pensar en su uso potencial como agentes terapéuticos. Así pues, en la actualidad disponemos de diferentes medicamentos y herramientas diagnósticas o de investigación básica derivados de toxinas. Esta revisión, basada en publicaciones realizadas en los últimos 10 años, busca proporcionar una visión actual del uso de algunas de estas moléculas como herramientas en diferentes campos de la biomedicina y la farmacia, y en su aplicación como nuevas alternativas terapéuticas o como modelos en el diseño de las mismas.
Descritores: Venenos de Moluscos
Toxina Tetânica
Responsável: CO56.3 - Biblioteca



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