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Id: biblio-1093557
Autor: Pineda, María E; Vargas, Alba M; Rodriguez-Acosta, Alexis.
Título: Alteraciones hemostáticas provocadas por el veneno de la culebra mapanare (Porthidium lansbergii hutmanni) en pacientes venezolanos / Haemostatic alterations caused by the venom of Lansberg's hognosed pitviper (Porthidium lansbergii hutmanni) in Venezuelan patients
Fonte: Rev. cuba. med. trop;71(2):e371, mayo.-ago. 2019. graf.
Idioma: en.
Resumo: Objective: to describe the haemostatic characteristics of the venom as well as the potency appraisal of the polyvalent antiophidic serum against haemotoxicity from Porthidium lansbergii hutmani experimental envenomation. Methods: Evaluation was performed of the venom's lethality, haemorrhagic activity, effects on coagulation and platelet aggregation, proteolytic activity, and neutralization by the commercial antivenom available in the country. Results: Several components with haemostatic activities were found in Porthidium l. hutmanni venom when a study of fibrinogenolytic, haemorrhagic and proteolytic activities was conducted of a pool of P.l.h venom. Porthidium l. hutmanni venom lacked the coagulant and defibrinating activities that are characteristic of bothropic venoms. Porthidium l. hutmanni venom showed very high haemorrhagic and anticoagulant activities. These findings could be related to the presence of multiple metalloproteases, which was evidenced in this study, and also the possible presence of phospholipases or other anticoagulant activity proteins that were not defined here. They inhibited platelet aggregation, suggesting that the venom had some proteins with marked effects on haemostasis. The commercial antivenom proved to be of little effectiveness in neutralizing the crude venom haemorrhagic activity. Conclusions: These toxins cause many physiopathological alterations in bitten patients, creating a clinical picture characterized by oedema, local and systemic haemorrhages, and even necrosis, comparable to that seen in bothropic envenomation. Porthidium l. hutmanni venom has no in vitro procoagulant activity, typical of bothropic venoms, suggesting there are variances in its protein conformation. Porthidium l. hutmanni venom is used for horse immunization. However, in order to preserve the patient's life, it is necessary to improve the immunization process to produce antivenom containing high avidity and specificity antibodies against the major toxins present in this venom. Porthidium l. hutmanni venom has demonstrated being a venom with high lethal, haemorrhagic, proteolytic and procoagulant activities, whose description will have enormous utility among clinicians who deal with these accidents in its geographical distribution areas(AU)

Objetivo: Describir las características hemostáticas del veneno y evaluar la potencia del suero polivalente antiofídico contra la hemotoxicidad provocada por el envenenamiento experimental por Porthidium lansbergii hutmanni. Métodos: Se realizó una evaluación de la letalidad, actividad hemorrágica, efectos en la coagulación y agregación plaquetaria, actividad proteolítica y neutralización por el antiveneno disponible comercialmente en el país. Resultados: Se encontraron varios componentes con actividad hemostática en el veneno de Porthidium l. hutmanni al realizarse un estudio de la actividad fibrinogenolítica, hemorrágica y proteolítica de una muestra de veneno de Porthidium l. hutmanni. El veneno de Porthidium l. hutmanni no mostró la actividad coagulante o defibrinante característica de los venenos botrópicos. El veneno de Porthidium l. hutmanni mostró una elevada actividad hemorrágica y anticoagulante. Estos resultados podrían estar relacionados con la presencia de múltiples metaloproteasas, la que quedó demostrado en el estudio, y también a la posible presencia de fosfolipasas u otras proteínas de actividad anticoagulante que no se definen en el mismo. La inhibición de la agregación plaquetaria sugiere que el veneno contiene algunas proteínas con un marcado efecto sobre la hemostasis. El antiveneno comercial mostró poca efectividad en la neutralización de la actividad hemorrágica del veneno crudo. Conclusiones: Estas toxinas provocan muchas alteraciones fisiopatológicas en las víctimas de mordeduras, creando un cuadro clínico caracterizado por edema, hemorragias locales y sistémicas e incluso necrosis comparable con la que ocurre en el envenenamiento botrópico. El veneno de Porthidium l. hutmanni no tiene la actividad procoagulante in vitro típica de los venenos botrópicos, lo que apunta a variaciones en su conformación proteica. El veneno de Porthidium l. hutmanni de utiliza en la inmunización de los caballos. Sin embargo, para preservar la vida del paciente, es necesario mejorar el proceso de inmunización con vistas a producir un antiveneno que contenga anticuerpos de elevada avidez y especificidad contra las principales toxinas presentes en el veneno. El veneno de Porthidium l. hutmanni ha mostrado ser un veneno de elevada actividad letal, hemorrágica, proteolítica y procoagulante, cuya descripción tendrá una enorme utilidad para los médicos que atienden esos accidentes en sus áreas de distribución geográfica(AU)
Descritores: Transtornos Hemostáticos/complicações
Venenos de Crotalídeos/efeitos adversos
Anticoagulantes/uso terapêutico
Limites: Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: biblio-1287094
Autor: Bhat, Shreesha K; Joshi, Manjunath B; Vasishta, Sampara; Jagadale, Rajesh N; Biligiri, Setlur G; Coronado, Monika A; Arni, Raghuvir K; Satyamoorthy, Kapaettu.
Título: P-I metalloproteinases and L-amino acid oxidases from Bothrops species inhibit angiogenesis
Fonte: J. venom. anim. toxins incl. trop. dis;27:e20200180, 2021. tab, graf.
Idioma: en.
Projeto: DBT/In- Bz; . CAPES; . FAPESP; . MCTI/CNPq/DBT.
Resumo: Snake venoms are composed of pharmacologically active proteins that are evolutionarily diverse, stable and specific to targets. Hence, venoms have been explored as a source of bioactive molecules in treating numerous diseases. Recent evidences suggest that snake venom proteins may affect the formation of new blood vessels. Excessive angiogenesis has been implicated in several pathologies including tumours, diabetic retinopathy, arthritis, inter alia. In the present study, we have examined the effects of P-I metalloproteinases isolated from Bothrops moojeni (BmMP-1) and Bothrops atrox (BaMP-1) and L-amino acid oxidases (LAAO) isolated from B. moojeni (BmLAAO) and B. atrox (BaLAAO) on biochemical and functional aspects of angiogenesis. Methods: P-I metalloproteinases and LAAO were purified from venom by molecular size exclusion and ion-exchange chromatography and subsequently confirmed using mass spectrometry. The P-I metalloproteinases were characterized by azocaseinolytic, fibrinogenolytic and gelatinase activity and LAAO activity was assessed by enzyme activity on L-amino acids. Influence of these proteins on apoptosis and cell cycle in endothelial cells was analysed by flow cytometry. The angiogenic activity was determined by in vitro 3D spheroid assay, Matrigel tube forming assay, and in vivo agarose plug transformation in mice. Results: P-I metalloproteinases exhibited azocaseinolytic activity, cleaved α and partially β chain of fibrinogen, and displayed catalytic activity on gelatin. LAAO showed differential activity on L-amino acids. Flow cytometry analysis indicated that both P-I metalloproteinases and LAAO arrested the cells in G0/G1 phase and further induced both necrosis and apoptosis in endothelial cells. In vitro, P-I metalloproteinases and LAAO exhibited significant anti-angiogenic properties in 3D spheroid and Matrigel models by reducing sprout outgrowth and tube formation. Using agarose plug transplants in mice harbouring P-I metalloproteinases and LAAO we demonstrated a marked disruption of vasculature at the periphery. Conclusion: Our research suggests that P-I metalloproteinases and LAAO exhibit anti-angiogenic properties in vitro and in vivo.(AU)
Descritores: Oxirredutases
Bothrops/fisiologia
Inibidores da Angiogênese
Venenos de Crotalídeos
Metaloproteases
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-829667
Autor: Nery, Neriane Monteiro; Luna, Karla Patrícia; Fernandes, Carla Freire Celedônio; Zuliani, Juliana Pavan.
Título: An overview of Bothrops erythromelas venom
Fonte: Rev. Soc. Bras. Med. Trop;49(6):680-686, Dec. 2016. graf.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico; . Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; . CNPq.
Resumo: Abstract This review discusses studies on the venom of Bothrops erythromelas published over the past 36 years. During this period, many contributions have been made to understand the venomous snake, its venom, and its experimental and clinical effects better. The following chronological overview is based on 29 articles that were published between 1979 and 2015, with emphasis on diverse areas. The complexity of this task demands an integration of multidisciplinary research tools to study toxinology. This science is in need of renewed conceptual and experimental platforms aimed at obtaining a profound understanding of the highly complex pathophysiology of snakebite envenoming and toxins isolated from snakes.
Descritores: Bothrops/classificação
Venenos de Crotalídeos/química
-Venenos de Crotalídeos/toxicidade
Venenos de Crotalídeos/farmacologia
Limites: Animais
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-1013309
Autor: Pérez-Gómez, Aline Stephanie; Monteiro, Wuelton Marcelo; João, Guilherme Augusto Pivoto; Sousa, Jose Diego de Brito; Safe, Izabella Picinin; Damian, Marcia Melo; Sachett, Jacqueline Almeida Gonçalves; Silva, Iran Mendonça da.
Título: Hemorrhagic stroke following viper bites and delayed antivenom administration: three case reports from the Western Brazilian Amazon
Fonte: Rev. Soc. Bras. Med. Trop;52:e20190115, 2019. tab, graf.
Idioma: en.
Resumo: Abstract Snakebites were included by the World Health Organization in their list of neglected diseases. In Latin America, most snakebites are caused by species of the Viperidae family, notably by the genus Bothrops. Bothrops atrox accounts for 90% of the cases of envenoming in the Brazilian Amazon. In this report, we present a series of three cases of snakebites that evolved with hemorrhagic stroke due to delays in the access to antivenom in the Brazilian Amazon, being fundamental for diagnosis to validate the clinical suspicion and make decisions that would improve the treatment and prognosis of the patients.
Descritores: Mordeduras de Serpentes/complicações
Acidente Vascular Cerebral/etiologia
Hemorragias Intracranianas/etiologia
Venenos de Crotalídeos/toxicidade
-Mordeduras de Serpentes/tratamento farmacológico
Brasil
Antivenenos/uso terapêutico
Bothrops
Limites: Humanos
Animais
Masculino
Feminino
Adolescente
Adulto
Idoso
Adulto Jovem
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: biblio-1020439
Autor: Carvalho, Letícia Helena de; Teixeira, Leda Fabiélen; Zaqueo, Kayena Delaix; Bastos, Jéssica Felix; Nery, Neriane Monteiro; Setúbal, Sulamita Silva; Pontes, Adriana Silva; Butzke, Diana; Cavalcante, Walter; Gallacci, Marcia; Fernandes, Carla Freire Celedônio; Stabeli, Rodrigo Guerino; Soares, Andreimar Martins; Zuliani, Juliana Pavan.
Título: Local and systemic effects caused by Crotalus durissus terrificus, Crotalus durissus collilineatus, and Crotalus durissus cascavella snake venoms in swiss mice
Fonte: Rev. Soc. Bras. Med. Trop;52:e20180526, 2019. tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Abstract INTRODUCTION: Crotalus envenomations cause serious complications and can be fatal without appropriate treatment. Venom isoforms present and inter/intraspecific variations in the venom composition can result in different symptoms presented by bites by snakes from the same species but from different geographical regions. We comparatively evaluated the local and systemic effects caused by Crotalus durissus terrificus (Cdt), C.d. collilineatus (Cdcolli), and C.d. cascavella (Cdcasc) envenomation. METHODS: Venom chromatography was performed. Proteolytic, phospholipase, and LAAO activities were analyzed. Edema, myotoxicity, hepatotoxicity, nephrotoxicity, and coagulation alterations were evaluated. RESULTS: The venom SDS-PAGE analyses found the presence of convulxin, gyroxin, crotoxin, and crotamine in Cdt and Cdcolli venoms. Crotamine was not present in the Cdcasc venom. Cdt, Cdcollli, and Cdcasc venoms had no proteolytic activity. Only Cdcasc and Cdt venoms had phospholipase activity. LAAO activity was observed in Cdcolli and Cdcasc venoms. Cdcolli and Cdcasc venoms caused 36.7% and 13.3% edema increases, respectively. Cdt venom caused a 10% edema induction compared to those by other venoms. All venoms increased TOTAL-CK, MB-CK, and LDH levels (indicating muscle injury) and ALT, AST, GGT, and ALP levels (markers of liver damage) and were able to induce a neuromuscular blockade. Urea and creatinine levels were also altered in both plasma and urine, indicating kidney damage. Only Cdcolli and Cdcasc venoms increased TAPP and TAP. CONCLUSIONS: Together, these results allow us to draw a distinction between local and systemic effects caused by Crotalus subspecies, highlighting the clinical and biochemical effects produced by their respective venoms.
Descritores: Crotalus/classificação
Venenos de Crotalídeos/toxicidade
Edema/induzido quimicamente
Rim/efeitos dos fármacos
Fígado/efeitos dos fármacos
-Ureia/sangue
Creatina Quinase/efeitos dos fármacos
Creatina Quinase/sangue
Creatinina/sangue
Modelos Animais
Edema/patologia
Eletroforese em Gel de Poliacrilamida
Fosfatase Alcalina/efeitos dos fármacos
Fosfatase Alcalina/sangue
Transaminases/efeitos dos fármacos
Transaminases/sangue
Rim/patologia
L-Lactato Desidrogenase/efeitos dos fármacos
L-Lactato Desidrogenase/sangue
Fígado/patologia
Camundongos
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-1155569
Autor: Silva, Flavio Souza; Ibiapina, Hiochelson Najibe Santos; Neves, Juliana Costa Ferreira; Coelho, Kerolaine Fonseca; Barbosa, Fabiane Bianca Albuquerque; Lacerda, Marcus Vinicius Guimarães; Sachett, Jacqueline Almeida Gonçalves; Malheiro, Adriana; Monteiro, Wuelton Marcelo; Costa, Allyson Guimarães.
Título: Severe tissue complications in patients of Bothrops snakebite at a tertiary health unit in the Brazilian Amazon: clinical characteristics and associated factors
Fonte: Rev. Soc. Bras. Med. Trop;54:e0374-2020, 2021. tab.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado do Amazonas.
Resumo: Abstract INTRODUCTION: Snakebites in the Brazilian Amazon are caused mostly by snakes from the Bothrops genus and envenomated patients may suffer from tissue complications. METHODS: This study aimed to identify risk factors for severe tissue complications (STC) in patients with Bothrops snakebite in the Amazonas state, Brazil. RESULTS: Snakebites that were classified as severe and affected female patients with comorbidities presented greater risks of developing STCs. In addition, hospitalizations of patients with STC exceeded 5 days. CONCLUSIONS: Clinical and epidemiological characteristics can prove essential for assessing the evolution of STC and clinical prognosis of patients with Bothrops snakebites.
Descritores: Mordeduras de Serpentes/complicações
Mordeduras de Serpentes/diagnóstico
Mordeduras de Serpentes/epidemiologia
Bothrops
Venenos de Crotalídeos
-Serpentes
Brasil/epidemiologia
Antivenenos
Fatores de Risco
Limites: Humanos
Animais
Feminino
Responsável: BR1.1 - BIREME


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Id: biblio-1170934
Autor: Rocco Daniela M; Reati Gustavo; Costa de Oliveira Vanessa; Lanari Laura C; Laskowicz Rodrigo D; de Roodt Adolfo R.
Título: Caracterización tóxica del veneno de Bothrops (Rhinocerophis) alternatus de diferentes regiones de la provincia de Córdoba (Argentina) / [The toxicity of venom of Bothrops (Rhinocerophris) alternatus in different areas of Cordoba State in Argentina].
Fonte: Rev. Fac. Cienc. Méd. (Córdoba);70(1):7-13, 2013.
Idioma: es.
Resumo: Snake venoms can show biochemical and toxicological variability even in specimens from the same specie. The geographical localization of the snakes is one of the factors that can influence those variations. By these reasons the venom from specimens of Bothrops (Rhinocerophis) alternatus ("crucera", "yararágrande"), one of the snakes of highest medical importance in Argentina, from three different regions of Córdoba was studied. Lehtal potency, hemorrhagic, coagulant on plasma and thrombin like activities as well as the electrophoretic patterns of venom from snakes of Calamuchita, Traslasierras and the East of the province were determined. The venom from the snakes of the three regions showed the characteristic activities of the venom of the majority of Bothrops, causing hemorrhage, hemostatic disturbances acting on plasma or directly on fibrinogen with a "thrombin like activity". The different samples were very similar regarding their biochemical characteristics and toxic potencies at difference of previous observations on venoms from the same specie in different regions of other provinces fro Argentina. Bivalent antivenom, the one used by the Provincial Ministry of Health to treat the bothropic accidents, neutralized in all the cases the toxic activities of the venom in very similar range of neutralizing potency.
Descritores: Antivenenos/farmacologia
Bothrops
Venenos de Crotalídeos/toxicidade
-Animais
Argentina
Bothrops/classificação
Coagulação Sanguínea/efeitos dos fármacos
Eletroforese em Gel de Poliacrilamida
Hemorragia/fisiopatologia
Hemorragia/induzido quimicamente
Venenos de Crotalídeos/antagonistas & inibidores
Venenos de Crotalídeos/química
Tipo de Publ: Resumo em Inglês
Artigo de Revista
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes


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Id: lil-644005
Autor: Ortiz, César; Lazo, Fanny; Bellido, Candy; Gonzales, Edgar; Yarlequé, Armando.
Título: Variaciones en las actividades enzimáticas del veneno de la serpiente Bothrops atrox "jergón", de tres zonas geográficas del Perú / Variation of the enzymatic activity of Bothrops atrox "jergon" snake venom from three geographic regions, Peru
Fonte: Rev. peru. med. exp. salud publica;29(2):198-205, abr.-jun. 2012. ilus, tab.
Idioma: es.
Resumo: Objetivos. Estudiar la variabilidad en la composición y actividades enzimáticas entre venenos de ejemplares adultos de Bothrops atrox. Materiales y métodos. Se emplearon venenos de serpientes adultas procedentes de Amazonas, Junín y Ucayali. A cada una de las muestras se les realizó el análisis del contenido proteico y del número de bandas por PAGESDS, así como las actividades de fosfolipasa A2, hemolítica indirecta, amidolítica, coagulante, hemorrágica y proteolítica sobre caseína y mediante zimograma; además, se hicieron ensayos de inmunodifusión y neutralización in vitro con el suero antibotrópico polivalente del Instituto Nacional de Salud de Perú. Resultados. Las actividades amidolítica, coagulante, hemorrágica, proteolítica mediante zimograma, fosfolipasa A2 y hemolítica indirecta fueron variables, evidenciándose en las tres últimas una mayor actividad en los venenos de Amazonas, mientras que en la cantidad de proteína, bandas electroforéticas y actividad proteolítica sobre caseína no se observaron diferencias. Con respecto a las pruebas de neutralización, 0,5 dosis del antiveneno fueron suficientes para neutralizar con eficacia (más del 50%) la actividad coagulante y fosfolipasa A2 de todas las muestras analizadas. Conclusiones. Algunas propiedades biológicas del veneno de ejemplares adultos de Bothrops atrox de Perú son variables, sin que ello afecte la neutralización in vitro por parte del suero antibotrópico polivalente sobre las actividades coagulante y fosfolipasa A2 del veneno.

Objectives. To study the variability in the composition and enzymatic activity of venom from adult Bothrops atrox specimens. Materials and methods. We used venoms from adult snakes from Amazonas, Junín and Ucayali. Each of the venom samples underwent analysis for protein and number of bands by pagesds. Phospholipase A2, hemolytic, amidolytic, coagulant, hemorrhagic activity were analyzed, also and proteolytic activity on casein and by zymogram. Additionally, immunodiffusion and neutralization assays in vitro were done with a polyvalent botropic serum from the national institute of health of Peru. Results. The amidolytic, coagulant, hemorrhagic, proteolytic by zymogram, phospholipase A2, and indirect hemolytic activity were variable, demonstrating increased activity in the venoms from Amazonas, regarding proteolytic by zymogram, phospholipase A2, and indirect hemolytic activity. While the amount of protein electrophoretic bands and proteolytic activity on casein did not demonstrated differences. Regarding neutralization tests, a 0.5 dose of antivenom was sufficient to effectively neutralize (>50%) the coagulant activity and phospholipase A2 of all samples analyzed. Conclusions. Some biological properties of the venom from adult Bothrops atrox of Peru are variable, without interference with the in vitro neutralization by the polyvalent botropic serum on coagulant and phospholipase A2 properties of the venom.
Descritores: Bothrops
Venenos de Crotalídeos/enzimologia
-Peru
Limites: Animais
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-625606
Autor: Mendoza, Julio C.; Vivas, Dan; Rodríguez, Edith; Inga, Rosio; Sandoval, Gustavo; Lazo, Fanny; Yarlequé, Armando.
Título: Eficacia experimental de anticuerpos IgY producidos en huevos, contra el veneno de la serpiente peruana Bothrops atrox / Experimental efficacy of IgY antibodies produced in eggs against the venom of the Peruvian snake Bothrops atrox
Fonte: Rev. peru. med. exp. salud publica;29(1):69-75, enero-mar. 2012. ilus.
Idioma: es.
Resumo: Objetivos. Desarrollar un protocolo de inmunización para producir inmunoglobulinas IgY de origen aviar contra el veneno de la serpiente peruana Bothrops atrox y evaluar la capacidad neutralizante. Materiales y métodos. Se inmunizaron seis gallinas de postura de la raza hy line brown con 500 μg/dosis de veneno de B. atrox en un periodo de dos meses. Cada semana, los huevos fueron colectados para el aislamiento de inmunoglobulinas IgY a partir de la yema, usando dos pasos consecutivos con αcido caprνlico y sulfato de amonio. La detecciσn de anticuerpos se realizσ por inmunodifusiσn doble mientras que el tνtulo y reactividad cruzada se determinaron por las técnicas de ELISA y Western blot. El cálculo de DL50 y de la DE50 del antiveneno IgY producido se realizó utilizando el método de Probits. Resultados. La masa de anticuerpos aislados fue de 8,5 ± 1,35 mg de IgY/mL de yema. Asimismo, la DE50 del antiveneno aviar fue calculada en 575 μL de antiveneno/mg de veneno. Adicionalmente, los ensayos de reactividad cruzada mostraron que el veneno de B. atrox comparte mas epνtopes comunes con el veneno de B. brazili (47%) que con otros veneno del mismo género, en tanto que los venenos de Lachesis muta (19%) y Crotalus durissus (12%) mostraron una baja reactividad cruzada. Conclusiones. Se ha obtenido IgY purificada contra el veneno de B. atrox con capacidad neutralizante y se ha demostrado su utilidad como herramienta inmunoanalítica para evaluar la reactividad cruzada con venenos de otras especies.

Objectives. To develop an immunization protocol in order to produce avian IgY immunoglobulins against Bothrops atrox Peruvian snake venom and to evaluate its neutralizing capacity. Materials and methods. Six Hy Line Brown hens were immunized each two weeks using 500μg/doses of B. atrox venom in a period of two months. Each week, eggs were collected for IgY isolation from yolk using two consecutive steps with caprilic acid and ammonium sulfate. Detection of IgY anti-B. atrox were performed by double immunodiffusion, whereas title and cross-reactivity were analyzed using ELISA and Western Blot technics, respectively. Furthermore, letal dose (DL50) and Medium Effective Dose (DE50) were obtained by Probit analysis. Results. As a result of this protocol, chicken IgY's were obtained in a concentration of 8,5 ± 1,35 mg/yolk mL. DE50 from avian antivenom was 575 μL/venom mg. Cross-reactivity studies showed Bothrops atrox venom share more commom epitopes with Bothrops brazili (47%) than others Bothrops venoms showing Lachesis muta (19%) and Crotalus durissus (12%) venoms a low crossing reactivity, instead. Conclusions. Using this procedure, we could purify chicken IgY with a neutralizant capacity of B. atrox venom which is comparable to the antivenom of equine origin and demonstrate its capacity as a immunoanalitical tool to evaluate the cross reactivity with others peruvian snakes.
Descritores: Antivenenos/biossíntese
Antivenenos/imunologia
Bothrops
Venenos de Crotalídeos/antagonistas & inibidores
Imunoglobulinas/biossíntese
Óvulo/imunologia
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-357018
Autor: Isla, Martín.
Título: Características bioquímicas y acción biológica de una hemorragina del veneno de Bothrops brazili / Biochemical characterization and biological action of an haemorrhagine from the Bothrops brazili venom
Fonte: An. Fac. Med. (Perú);64(3):159-166, jul. 2003. tab, graf.
Idioma: es.
Resumo: OBJETIVO: Caracterizar la hemorragina aislada de B. brazili y determinar si el suero antibotrópico polivalente es capaz de neutralizarlo. MATERIAL Y MÉTODOS: Se ha purificado una hemorragina del veneno de Bothrops brazili a través de dos pasos cromatográficos en Sephadex G-100 y CM Sephadex C-50, usando buffer acetato de amonio 0,05M pH 7. En este último sistema, la proteína fue eluida con NaCl 0,3M y el análisis electroforético en PAGE-SDS mostró una sola banda proteica. Usando caseína como substrato se determinó una purificación de 8,4 veces, habiéndose calculado la dosis hemorrágica mínima (DHM) en 6,61 ug usando ratones albinos. RESULTADOS: El análisis de carbohidratos asociados a la hemorragina reveló un contenido de 8,05 por ciento de hexosas, 11,62 por ciento de hexosamina y 0,69 por ciento de ácido siálico, en tanto que su resistencia térmica fue registrada hasta 50ºC por 10 minutos, ya que temperaturas mayores a inactivaron. La hemorragina fue muy inestable a pH 5,0 mientras que el tratamiento con EDTA, ácido glutámico y glutatión 5 mM también produjeron una severa inhibición, tanto en su acción hemorrágica.
Descritores: Imunoeletroforese
Viperidae
Bothrops
Imunodifusão
Venenos de Crotalídeos
Limites: Animais
Responsável: PE13.1 - Oficina de Biblioteca, Hemeroteca y Centro de Documentación



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