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Id: biblio-1052269
Autor: Feng, Shoushuai; Hou, Shaoxiang; Huang, Xing; Fang, Zheng; Tong, Yanjun; Yang, Hailin.
Título: Insights into the microbial community structure of anaerobic digestion of municipal solid waste landfill leachate for methane production by adaptive thermophilic granular sludge
Fonte: Electron. j. biotechnol;39:98-106, may. 2019. tab, graf, ilus.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Social Development Projects of Jiangsu Province; . Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education; . Key Laboratory of Industrial Biotechnology, Ministry of Education; . Priority Academic Program Development of; . Jiangsu Higher Education Institutions, and Program of Introducing; . Talents of Discipline to Universities.
Resumo: BACKGROUND: The amount of municipal solid waste (MSW) gradually increased along with the rapid development of modern cities. A large amount of landfill leachate are generated with excessive chemical oxygen demand (COD), which create a great deal of pressure on the environment-friendly treatment process. Anaerobic digestion is an ideal technique to solve the above problem. RESULTS: A thermophilic granular sludge was successfully adapted for anaerobic digestion of MSW leachate (from an aging large-scale landfill) for methane production. The COD degradation efficiency improved by 81.8%, while the methane production rate reached 117.3 mL CH4/(g VS d), which was 2.34-fold more than the control condition. The bacterial and archaeal communities involved in the process were revealed by 16S rRNA gene high-throughput pyrosequencing. The richness of the bacterial community decreased in the process of thermophilic granular sludge, while the archaeal community structure presented a reverse phenomenon. The bacterial genus, Methanosaeta was the most abundant during the mesophilic process, while Methanobacterium, Methanoculleus, Methanosaeta and Methanosarcina were more evenly distributed. The more balanced community distribution between hydrogenotrophic and acetotrophic methanogens implied a closer interaction between the microbes, which further contributed to higher methane productivity. The detailed relationship between the key functional communities and anaerobic digestion performances were demonstrated via the multivariate canonical correspondence analysis. Conclusions: With the assistance of adaptive thermophilic granular sludge, microbial community structure was more evenly distributed, while both of COD degradation rate and methane production was improved during anaerobic digestion of MSW landfill leachate.
Descritores: Bactérias Anaeróbias/metabolismo
Resíduos Sólidos
Digestão Anaeróbia
Tratamento de Lodos
Metano/metabolismo
-Esgotos/microbiologia
Bactérias Anaeróbias/isolamento & purificação
Poluentes Químicos da Água
Reação em Cadeia da Polimerase
Área Urbana
Biocombustíveis
Análise da Demanda Biológica de Oxigênio
Temperatura Alta
Anaerobiose
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1052032
Autor: Reyes-Contreras, Carolina; Leiva, Ana María; Vidal, Gladys.
Título: Evaluation of triclosan toxic effects on the methanogenic activity
Fonte: Electron. j. biotechnol;39:61-66, may. 2019. graf, tab.
Idioma: en.
Projeto: CONICYT (Chile).
Resumo: BACKGROUND: Triclosan (TCS) is an antimicrobial agent widely used in health care and consumer products. This compound is present in sludge of wastewater treatment plants (WWTPs), and because of its bactericidal characteristics, it can inhibit the methanogenic activity in anaerobic digestion (AD) technology. The aim of this study was to evaluate the toxic effects of TCS on the methanogenic activity. RESULTS: Batch anaerobic reactors were used with TCS concentrations of 7.8, 15.7, 23.5, and 31.4 mg/L. These assays consisted in three successive feedings (I, II, and III), wherein the sludge was exposed to each TCS concentration and volatile fatty acid (VFA) substrate. For evaluation of the residual sludge activity during feeding III, only VFA was used. The results showed that the increase in TCS concentrations correlated with the reduction in methane (CH4) production. In this case, the minimum values were achieved for TCS concentration of 31.4 mg/L with CH4 levels between 101.9 and 245.3 during feedings I, II, and III. Regarding the effect of TCS on VFA consumption, an inhibitory effect was detected for TCS concentrations of 23.5 and 31.4 mg/L, with concentrations of acetic, butyric, and propionic acids at the end of the assay (37 d) between 153.6 and 206.8, 62.5 and 60.1, and 93.4 and 110 mg/L, respectively. Regarding the removal of TCS during AD, these values were above 47%. Conclusion: TCS is an inhibitor of methanogenic activity with a decrease between 63 and 70% during the different feedings. The CH4 production was not recovered during feeding III, with inhibition percentages of 21­72%.
Descritores: Triclosan/toxicidade
Digestão Anaeróbia
Metano/metabolismo
Anti-Infecciosos/toxicidade
-Esgotos
Estações de Tratamento de Águas Residuárias
Cromatografia Líquida de Alta Pressão
Ácidos Graxos Voláteis
Anaerobiose
Responsável: CL1.1 - Biblioteca Central


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Id: lil-38805
Autor: Bartone, Carl R.
Título: Reutilización de águas residuales en las lagunas de estabilización de San Juan de Miraflores: repercusiones sanitarias, ambientales y socioeconomicas / Reuse of wastewater at the San Juan de Miraflores stabilization ponds: public health, enviromental, and socioeconomic implications
Fonte: Bol. Oficina Sanit. Panam;101(5):[425-51], nov. 1986. tab, ilus.
Idioma: es.
Resumo: La reutilización de aguas residuales para fines de riego es común en muchas zonas áridas y semiáridas de América Latina. Sin embargo, esa práctica puede entrañar riesgos para la salud. Es esencial que en cualquier proyeto de reutilización se adopte por lo menos el mínimo de medidas eficaces de contol sanitario necesarias para mitigar los posibles riesgos para la salud pública. A fin de determinar la posibilidad de ejercer ese control por medio de lagunas de estabilización de aguas residuales en las condiciones desérticas que predominan en la costa peruana, el Centro Panamericano de Ingeniería Sanitaria y Ciencias del Ambiente ha realizado extensos estudios de campo y laboratorio en las lagunas de estabilización del proyecto de reutilización de aguas residuales de San Juan de Miraflores en la zona periférica del sur de Lima. En este artículo se ofrece una descripción general de esos estudios. En la primera fase del trabajo, iniciado en 1977, se evaluaron los efectos logrados con cuatro conjuntos de dos lagunas en serie que recibieron cargas de desechos orgánicos de 400, 600, 800 y 1000 kilogramos de demanda bioquímica de oxígeno (DBO) por hectárea por día. Entre otras cosas, los resultados indicaron que, si bien la eliminación de DBO era constantemente alta, no era posible eliminar todos los protozoarios patógenos en las series de dos lagunas con un periódo mínimo de retención de 5,5 días; que 36 días de retención no eran suficientes para destruir todas las especies de Salmonella, y que algunos de los serotipos de Salmonella aislados eran resistentes a los antibióticos. La segunda fase del trabajo, en la que se emplearon dos conjuntos de tres lagunas en serie y un conjunto de cuatro lagunas en serie, se concentró principalmente en la supervivencia de los agentes patógenos...
Descritores: Esgotos/análise
/métodos
ACONITINE0ABDOMINAL INJURIES/métodos
Engenharia Sanitária
-Peru
Limites: Humanos
Responsável: BR1.1 - BIREME


  4 / 105 LILACS  
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Texto completo SciELO Saúde Pública
Texto completo
Id: biblio-1101589
Autor: Massa, Kaio Henrique Correa; Chiavegatto Filho, Alexandre Dias Porto.
Título: Saneamento básico e saúde autoavaliada nas capitais brasileiras: uma análise multinível / Basic sanitation and self-reported health in Brazilian capitals: a multilevel analysis
Fonte: Rev. bras. epidemiol;23:e200050, 2020. tab.
Idioma: pt.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: RESUMO: Objetivo: Analisar a associação entre os determinantes contextuais referentes ao saneamento básico e a autoavaliação de saúde nas capitais brasileiras. Métodos: Analisaram-se 27.017 adultos (≥ 18 anos) residentes nas 27 capitais brasileiras em 2013, utilizando dados da Pesquisa Nacional de Saúde (PNS). Ajustaram-se modelos multiníveis logísticos bayesianos para analisar a associação entre a autoavaliação de saúde e a cobertura dos serviços de saneamento básico (rede de esgoto, abastecimento de água e coleta de lixo), controlando a análise por fatores individuais (primeiro nível do modelo) e renda per capita da cidade de residência (segundo nível). Resultados: A maior cobertura de serviços de saneamento básico esteve consistentemente associada à melhor percepção da saúde, mesmo após o controle pelas características individuais e contextuais. Observou-se menor chance de autoavaliação ruim de saúde entre indivíduos que viviam em capitais com média (odds ratio - OR = 0,59; intervalo de confiança - IC95% = 0,57 - 0,61) e alta (OR = 0,61; IC95% = 0,57 - 0,66) cobertura da rede de coleta de esgoto; média (OR = 0,77; IC95% = 0,71 - 0,83) cobertura de serviço de abastecimento de água; e alta (OR = 0,78; IC95% = 0,69 - 0,89) proporção de coleta de lixo. Conclusão: A associação positiva entre melhores condições de saneamento básico e a autoavaliação da saúde, independentemente dos fatores individuais e das condições socioeconômicas do local de residência, confirma a necessidade de se considerar o saneamento básico na elaboração de políticas de saúde.

ABSTRACT: Objective: This study aimed to analyze the association between the contextual determinants related to basic sanitation and self-reported health in Brazilian capitals. Methods: The sample consisted of 27,017 adults (≥18 years) residing in the 27 Brazilian capitals in 2013, from the National Health Survey (PNS). The association between self-reported health and sanitation (sewage system, water supply and garbage collection) was analyzed using Bayesian multilevel models, controlling for individual factors (first level of the model) and area-level socioeconomic characteristics (second level). Results: We found a consistent association between better self-reported health and better sanitation levels, even after controlling for individual and contextual characteristics. At the contextual level, lower odds of poor self-reported health was observed among those living in areas with medium (OR = 0.59, 95%CI 0.57 - 0.61) or high (OR = 0.61, 95%CI 0.57 - 0.66) sewage system level; medium (OR = 0.77, 95%CI 0.71 - 0.83) coverage of water supply; and high (OR = 0.78, 95%CI 0.69 - 0.89) garbage collection level. Conclusion: The positive association between better sanitation conditions and health, independently of the individual factors and the socioeconomic characteristics of the place of residence, confirms the need to consider sanitation in the planning of health policies.
Descritores: Saneamento/estatística & dados numéricos
Nível de Saúde
-Esgotos
Fatores Socioeconômicos
População Urbana
Brasil
Inquéritos Epidemiológicos
Análise Multinível
Autorrelato
Política de Saúde
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Adolescente
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Texto completo
Id: biblio-886775
Autor: ZOCCHE, JAIRO J; ROHR, PAULA; DAMIANI, ADRIANI P; LEFFA, DANIELA D; MARTINS, MIRIAM C; ZOCCHE, CAROLINE M; TEIXEIRA, KARINA O; BORGES, GABRIELA D; JESUS, MAIELLEN M DE; SANTOS, CARLA E I DOS; DIAS, JOHNNY F; ANDRADE, VANESSA M DE.
Título: Elemental composition of vegetables cultivated over coal-mining waste
Fonte: An. acad. bras. ciênc;89(3,supl):2383-2398, 2017. tab.
Idioma: en.
Resumo: ABSTRACT We assessed elemental composition of the liver in mice subjected to one-time or chronic consumption of the juice of vegetables cultivated in a vegetable garden built over deposits of coal waste. Lactuca sativa L. (lettuce), Beta vulgaris L. (beet), Brassica oleracea L. var. italica (broccoli) and Brassica oleracea L. var. acephala (kale) were collected from the coal-mining area and from a certified organic farm (control). Elemental composition was analyzed by particle-induced X-ray emission (PIXE) method. Concentrations of Mg, S, and Ca of mice subjected to one-time consumption of broccoli and concentrations of these same elements plus Si of mice receiving kale were higher in the coal-mining area. Concentrations of P, K, and Cu were increase after chronic consumption of lettuce from the coal-mining area, whereas the levels of Si, P, K, Fe, and Zn were higher in the group consuming kale from the coal-mining area. Our data suggests that people consuming vegetables grown over coal wastes may ingest significant amounts of chemical elements that pose a risk to health, since these plants contain both essential and toxic metals in a wide range of concentrations, which can do more harm than good.
Descritores: Esgotos/química
Poluentes do Solo/análise
Verduras/química
Contaminação de Alimentos/análise
Minas de Carvão
Fígado/efeitos dos fármacos
-Poluentes do Solo/toxicidade
Verduras/toxicidade
Eliminação de Resíduos Líquidos
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-962075
Autor: Arévalo-Arbeláez, Ángela J; Bedoya-Urrego, Katherine; Cabarcas-Jaramillo, Felipe; Alzate-Restrepo, Juan F.
Título: Descripción de la microbiota bacteriana residente en el biosólido generado en la planta de tratamiento de aguas residuales San Fernando. Itagüí, Colombia / Description of bacterial microbiota in biosolids generated in the San Fernando wastewater treatment plant. Itagüí, Colombia
Fonte: Rev. salud pública;19(6):806-813, nov.-dic. 2017. tab, graf.
Idioma: es.
Resumo: RESUMEN Objetivo Caracterizar la microbiota bacteriana presente en los biosólidos generados en una de las plantas de tratamiento de aguas residuales más grande de Colombia. Materiales y Métodos Se utilizó la plataforma de secuenciamiento 454 de la compañía Roche para secuenciar las regiones variables V1-V3 y V6-V9 del marcador molecular 16S rRNA y caracterizar la microbiota. Adicionalmente, se aplicaron estrategias filogenéticas para la identificación de especies bacterianas de importancia. Resultados Nuestros análisis muestran que los Phyla más abundantes son Chloro-flexi, Proteobacteria, Bacteroidetes, Actinobacteria y Firmicutes. Los géneros clasificados más abundantes fueron Pseudomonas, Dysgonomonas y Proteiniphilum. Sin embargo, el grupo dominante según la región variable V1-V3 es una Anaerolineaceae que no se ajusta a las especies descritas para esta familia. Conclusiones En las muestras de biosólido analizadas predominan bacterias ambientales que participan en los procesos de estabilización de la materia orgánica durante los tratamientos biológicos de tipo secundario y la digestión anaerobia. Se detectaron secuencias de especies dentro de la familia Anaerolineaceae, los análisis filogenéticos muestran que probablemente se trata de especies no descritas. En el momento del estudio, se encontró que en el sistema de digestión anaerobia se genera biosolido con una baja carga de bacterias potencialmente patógenas.(AU)

ABSTRACT Objective To describe bacterial microbiota in the biosolids generated in one of the largest wastewater treatment plants of Colombia. Materials and Methods Using NGS technology, 16S rRNA Gene Amplicon libraries were amplified and sequenced. The Roche 454 FLX Titanium platform was used, while the V1-V3 and V6-V9 hypervariable regions were amplified and analyzed independently. Amplicon processing and bacterial classification were performed using the AmpliconNoise pipeline and the RDP Classifier tool. Results The analysis showed that the most dominant Phyla in the biosolids were Chlo-roflexi, Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. The most dominant genera were Pseudomonas, Dysgonomonas and Proteiniphilum; however, the dominant group according in the V1-V3 variable region was Anaerolineaceae, which does not conform to the species described for this family. Pathogenic bacteria such as Salmonella and E. coli/Shigella were not detected in the studied biosolid sample. Conclusions In the biosolids samples analyzed, environmental bacteria involved in organic matter stabilization processes during secondary biological treatments and anaerobic digestion were predominant. One of the dominant species in this sludge is a novel species of the Anaerolineaceae group. At the time of the study, it was found that the anaerobic digester system was able to maintain pathogenic bacteria at very low concentrations.(AU)
Descritores: Esgotos/análise
RNA Ribossômico 16S
Purificação da Água/normas
Microbiota
-Colômbia
Técnicas de Diagnóstico Molecular
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Texto completo
Id: biblio-1118017
Autor: QUEIROZ, Amanda Caroliny Marques de; SANTOS, Wânia Mendonça dos; MENDONÇA, Rita de Cássia Almeida de; SANTOS, Rosana Ingrid Ribeiro dos; SILVA, Thiago Carvalho da; DOMINGUES, Felipe Nogueira; RÊGO, Aníbal Coutinho do.
Título: Effects of inclusion of patauá, Oenocarpus bataua meal on elephant grass silage
Fonte: Acta amaz;50(2):101-107, abr - jun. 2020.
Idioma: en.
Resumo: Elephant grass silages have high moisture content, causing nutrient loss, mainly by effluent production. The use of moisture absorbent additives, such as patauá, Oenocarpus bataua meal, can be a strategy to reduce losses in these silages. The aim of this study was to determine the fermentation losses, microbiological composition, fermentative characteristics, and chemical composition of elephant grass silage with increasing levels of inclusion of patauá meal. The experiment was conducted in a completely randomized design, with four concentrations of patauá meal (7%, 14%, 21%, and 28%, based on fresh matter) and a control without patauá, and five replicates. The elephant grass was collected manually after 60 days of growth. After chopping, the grass was homogenized, mixed with the patauá meal according to the established treatments, and ensiled in experimental silos of 15 L. The inclusion of patauá meal in elephant grass silage had positive effects on the chemical composition and effluent loss. It significantly increased dry matter, organic matter, crude protein, nitrogen insoluble in neutral detergent and acid, ethereal extract, lignin, and non-fibrous carbohydrates of the silages. It also reduced effluent losses, neutral and acid detergent fiber contents, and cellulose. Our findings contribute towards reducing the environmental impact caused by the production of effluents during ensilage of tropical grasses and providing a potential use for the residue from the production of patauá oil, thus avoiding disposal in the environment. (AU)
Descritores: Excipientes Farmacêuticos
Esgotos
Pennisetum
Umidade
Responsável: BR6.1 - BCS - Biblioteca de Ciências da Saúde


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Texto completo SciELO Brasil
Texto completo
Id: biblio-888790
Autor: Comerlato, J; Souza-Campos, F; Souza-Arantes, T; Roos-Kulmann, M I; Trindade-Oliveira, M; Rosado-Spilki, F; Guedes-Frazzon, A P; Roehe, P M; Franco, A C.
Título: Distribution and genetic diversity of the human polyomaviruses JC and BK in surface water and sewage treatment plant during 2009 in Porto Alegre, Southern Brazil / Distribuição e diversidade genética dos poliomavírus humanos JC e BK em águas superficiais e de estação de tratamento de esgoto durante 2009 em Porto Alegre, sul do Brasil
Fonte: Braz. j. biol;77(3):459-468, July-Sept. 2017. tab, graf.
Idioma: en.
Resumo: Abstract The human polyomaviruses JC and BK (JCPyV and BKPyV) are ubiquitous, species-specific viruses that belong to the family Polyomaviridae. These viruses are known to be excreted in human urine, and they are potential indicators of human wastewater contamination. In order to assess the distribution of both JCPyV and BKPyV in urban water samples collected from a sewage treatment plant (STP) and from a canalized water stream of Porto Alegre, Brazil, two nested-PCR assays were optimized and applied to the samples collected. The amplicons obtained were submitted to sequencing, and the sequences were analyzed with sequences of human polyomaviruses previously deposited in GenBank. Twelve out of 30 water samples (40%) were JCPyV positive, whereas six samples (20%) were BKPyV positive. The sequencing results confirmed the presence of JCPyV subtypes 1 and 3, whereas only BKPyV Ia and Ib were found. This study shows for the first time the presence of human polyomaviruses in surface water and in samples collected in a sewage treatment plant in southern Brazil.

Resumo Os poliomavírus humanos JC e BK (JCPyV e BKPyV) são virus ubíquos, espécie-específicos, pertencentes à família Polyomaviridae. Estes vírus são conhecidos por serem excretados pela urina humana, sendo considerados potenciais indicadores de contaminação por águas residuais urbanas. Buscando acessar a distribuição de JCPyV e BKPyV em amostras de águas coletadas de uma estação de tratamento de esgoto e de um arroio canalizado de Porto Alegre, Brasil, duas técnicas de nested-PCR foram otimizadas e aplicadas às amostras coletadas. Os amplificados obtidos foram submetidos ao sequenciamento e suas sequências analisadas com base em sequências de poliomavírus humanos previamente depositadas no GenBank. Doze de 30 amostras de água (40%) foram positivas para JCPyV, enquanto 6 amostras (20%) foram positivas para BKPyV. Os resultados do sequenciamento confirmaram a presença dos subtipos 1 e 3 de JCPyV, enquanto apenas os BKPyV Ia e Ib foram encontrados. Este estudo demonstra pela primeira vez a presença de poliomavírus humanos em águas superficiais e em amostras coletadas em uma estação de tratamento de esgoto na região sul do Brasil.
Descritores: Esgotos/virologia
Vírus BK/isolamento & purificação
Vírus BK/genética
Vírus JC/isolamento & purificação
Vírus JC/genética
Água Doce/virologia
-Variação Genética
Brasil
Reação em Cadeia da Polimerase
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: biblio-1013743
Autor: Martins, Felippe Danyel Cardoso; Ladeia, Winni Alves; Toledo, Roberta dos Santos; Garcia, João Luis; Navarro, Italmar Teodorico; Freire, Roberta Lemos.
Título: Surveillance of Giardia and Cryptosporidium in sewage from an urban area in Brazil / Monitoramento de Giardia e Cryptosporidium em esgoto de uma área urbana no Brasil
Fonte: Rev. bras. parasitol. vet;28(2):291-297, Apr.-June 2019. tab.
Idioma: en.
Resumo: Abstract Cryptosporidium and Giardia are protozoan parasites that cause diarrhea in humans and animals. Molecular characterization of these pathogens in sewage may provide insight on their occurrence and prevalence in Brazil. This study aimed to investigate the presence of Giardia and Cryptosporidium in raw and treated sewage from Londrina, Paraná, Brazil. Samples were collected every two weeks during a year. Samples were concentrated, then DNA was extracted and subjected to a nested PCR targeting the Giardia 18S rRNA gene and the Cryptosporidium 18S rRNA gene. Species of Cryptosporidium were characterized by restriction fragment length polymorphism (RFLP). All raw sewage and 76% of the treated sewage were positive for Giardia; 84% of raw sewage samples and 8% of treated sewage were positive for Cryptosporidium. C. muris, C. hominis, C. baileyi, C. parvum and C. suis were detected in 100%, 19%, 9%, 9% and 4% of raw sewage, respectively. C. muris was the only species found in treated sewage. Multiple species of Cryptosporidium were present in 19.04% of the raw sewage. Treated sewage water can pose a threat to human health. The speciation of Cryptosporidium revealed the presence of non-common zoonotic species as C. suis and C. muris.

Resumo Cryptosporidium e Giardia são protozoários causadores de diarreia em animais e humanos. A caracterização molecular destes protozoários em esgoto pode prover dados ainda desconhecidos da ocorrência de espécies. O objetivo do presente estudo foi monitorar a ocorrência de Giardia e espécies de Cryptosporidium em esgoto bruto e tratado em uma estação de tratamento de esgoto (ETE) de Londrina, Paraná. Amostras de esgoto bruto e tratado foram coletadas no período de um ano, com periodicidade quinzenal. A ocorrência destes protozoários foi caracterizada por meio de concentração das amostras e posterior extração de DNA seguida de nested-PCR para amplificação de fragmentos dos genes 18S rRNA de Giardia e 18S rRNA de Cryptosporidium. A caracterização das espécies de Cryptosporidium foi realizada por meio de análise por polimorfismo de comprimento do fragmento de restrição (RFLP) dos produtos obtidos. Foram coletadas no total 25 amostras de cada, esgoto bruto e esgoto tratado. Para Giardia, todas as amostras de esgoto bruto e 76% das de esgoto tratado foram positivas. Cryptosporidium esteve presente em 84% das amostras de esgoto bruto e em 8% do tratado. No esgoto tratado foi encontrado apenas C. muris, já nas amostras de esgoto bruto foram encontradas cinco espécies: C. muris, C. hominis, C. baileyi, C. suis e C. parvum em 100%, 19%, 9%, 9% e 4%, respectivamente. A presença de espécies mistas foi observada em 19,04% das amostras. A presença de Giardia e Cryptosporidium em esgoto tratado pode pôr em risco a saúde humana. A discriminação de espécies de Cryptosporidium revelou a presença de espécies zoonóticas incomuns como C. suis e C. muris.
Descritores: Esgotos/parasitologia
RNA Ribossômico 18S/genética
Cryptosporidium/isolamento & purificação
Giardia/isolamento & purificação
-População Urbana
Brasil
Reação em Cadeia da Polimerase
Cryptosporidium/genética
Giardia/genética
Responsável: BR1.1 - BIREME


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Miagostovich, Marize Pereira
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Id: biblio-1100644
Autor: Silva-Sales, Marcelle; Caldas, Mariana Seglia; Fioretti, Julia Monassa; Rocha, Monica Simões; Fumian, Túlio Machado; Miagostovich, Marize Pereira.
Título: Detection of salivirus in raw sewage samples in Rio de Janeiro, Brazil
Fonte: Rev. patol. trop;49(2), 2020.
Idioma: en.
Resumo: Gastroenteric viruses are important pathogens related to cases of acute gastroenteritis, affecting millions of people worldwide with a major impact on children under five in developing countries. The introduction of metagenomic approach techniques in the 2000s has allowed the description of new viruses, among them Salivirus, which has been associated worldwide with cases of diarrhea. This study aimed to detect salivirus in raw sewage samples from a wastewater treatment plant (WWTP) collected between June 2013 and May 2014 in Rio de Janeiro, Brazil. Fifty-two samples collected weekly were tested by using a real-time quantitative PCR (qPCR). Salivirus genome was detected in 71.1% (37/52) of the samples, with viral concentration ranging from 7.56 x 104 to 7.20 x 106 genomic copies per liter. Higher viral loads were detected in the summer and fall of 2014, although these data were not sufficient to infer seasonality for this virus. The high prevalence of salivirus in sewage samples highlights the importance of viral research in wastewater to generate data on salivirus circulation, increasing understanding regarding its dissemination in the population.
Descritores: Esgotos
Reação em Cadeia da Polimerase em Tempo Real
Gastroenterite/epidemiologia
Responsável: BR15.1 - Biblioteca de Ciências Biomédicas



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