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Pesquisa : D23.050 [Categoria DeCS]
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Id: lil-591532
Autor: Aria, L; Guillén, Y; Rojas, A; Acosta, M. E; Roig, C; Meza, T; Carpinelli, M; Ferreira, L.
Título: Concordancia de antígenos de dengue en el ELISA de captura de IgM (MAC ELISA) en el IICS-UNA / Concordance of dengue antigens M antibody-capture ELISA (MAC ELISA) in the IICS-UNA
Fonte: Mem. Inst. Invest. Cienc. Salud (Impr.);8(2):34-38, dic. 2010. tab.
Idioma: es.
Resumo: El dengue es una enfermedad aguda grave considerada actualmente como infección reemergente, cuyo vector principal es el Aedes aegypti. En Paraguay en el 2007 fueron reportados 28.181 casos, 55 se clasificaron como fiebre hemorrágica del dengue de los cuales 7 fallecieron. El 90% de los casos fueron de Asunción y del Departamento Central,10% del resto del país. En los últimos años se han desarrollado diferentes sistemas inmunoenzimáticos para el diagnóstico del dengue, entre ellos el ELISA de captura de IgM (MAC ELISA). El objetivo de este estudio observacional analítico de corte transverso fu ecomparar la prueba del MAC ELISA desarrollada en el Instituto de Investigaciones en Ciencias de la Salud (IICS) utilizando antígenos suministrados por el Instituto Pedro Kouri(IPK) de Cuba y el Evandro Chagas de Brasil, con el kit comercial ELISA IgM por capturapara virus del dengue (Focus Diagnostics Inc. Cypress, CA, USA). Fueron seleccionados alazar 92 sueros de pacientes codificados que concurrieron al IICS con sospecha de dengue, respetándose la confidencialidad de los mismos. La concordancia obtenida fue del94.6% (Índice Kappa: 0.891) utilizando el antígeno del IPK y 96.7% (Índice Kappa:0.9350) con el antígeno del Evandro Chagas, mostrándose alta significancia estadística(p<0.00001) en ambos casos. La excelente concordancia obtenida con los dos antígenos indica que los mismos pueden ser utilizados indistintamente en la prueba del MAC ELISA desarrollada en el IICS, a fin de apoyar el diagnóstico del dengue a menor costo y quesería de producción local.

Dengue is an acute disease currently considered a re-emerging infection, whose main vector is Aedes aegypti. In 2007, 28,181 cases were reported in Paraguay, 55 were classified as dengue hemorrhagic fever and seven of them died. Ninety percent of the cases were from Asunción and the Central Department and the remaining 10% from the rest of the country. In recent years various immunoenzymatic systems have been developed immunoassay for the diagnosis of dengue, including the M antibody captureELISA (MAC ELISA). The aim of this cross-sectional observational study was to compare the MAC ELISA test developed at the Instituto de Investigaciones en Cienciad de la Salud(IICS) using antigens supplied by the Instituto Pedro Kouri (IPK) of Cuba and Evandro Chagas of Brazil with a commercial kit of M antibody capture ELISA for dengue virus (Focus Diagnostics Inc. Cypress, CA, USA). Ninety two coded serum samples wererandomly selected from patients who attended the IICS with suspected dengue, respecting their confidentiality. The concordance obtained was 94.6% (Kappa Index: 0.891) using the IPK antigen and 96.7% (Kappa index: 0.9350) with the antigen from Evandro Chagas showing high statistical significance (p<0.00001) in both cases. The excellent concordance obtained with the two antigens indicates that they can be used indistinctly in the MAC ELISA test developed in the IICS to support the diagnosis of dengue at a lower cost and would be locally produced.
Descritores: Antígenos
Dengue
Responsável: PY3.1 - Biblioteca


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Id: biblio-1015221
Autor: Infante, Natalia S; Novak, Ivón T C.
Título: Ultraestructura del compartimiento clase II en macrófagos humanos en rosetas macrófago-linfocitarias autólogas / Ultrastructure of the class II compartment in human macrophages in autologous macrophage-lymphocyte rosettes
Fonte: ARS med. (Santiago, En línea);40(1):11-18, 2015. ilus.
Idioma: es.
Resumo: Introducción: El procesamiento y presentación de antígenos está involucrado en el fenómeno de múltiples sinapsis inmunológicas de la Roseta Macrófago-Linfocitaria humana (RML) entre macrófagos derivados de monocitos y linfocitos T CD4+ de cultivos autólogos leucocitarios totales extraídos de la sangre; aquí los antígenos autólogos de los neutrófilos apoptóticos son presentados por la vía endocítica o vía Clase II. El Compartimiento Clase II (CCMII) ha sido caracterizado en células B y células dendríticas en modelos murinos. Objetivo: estudiar la evolución ultraestructural de la organización espacial CCMII en macrófagos en el fenómeno de RML humana. Métodos: Se utilizaron muestras de sangre humana sana, anticoagulada con heparina (n=10) donadas por Banco de Sangre, UNC, en anonimato. Cultivos leucocitarios autólogos en medio TC199 (SIGMA, St. Louis, MO). Se tomaron muestras de cultivo celular a: 1, 2, 3, 20, 48 y 96 horas. Se aplicó la técnica de RML. Las citopreparaciones se sometieron a técnicas de procesamiento para su estudio ultraestructural con el MET: Zeiss LEO-906E. Resultados: Observamos cuerpos multivesiculares, multilaminares y tubulares en la organización espacial del CCMII a lo largo del tiempo de cultivo. Estructuras tubulares aparecieron a las 48 horas de cultivo. Se concluye que organización espacial del CCMII toma diversos aspectos en coincidencia con la ocurrencia de transformación macrofágica en cultivo y su rol como célula presentadora de antígenos (CPA) en RMLs. Dado el origen autólogo de los antígenos presentados postulamos que el perfil de los macrófagos en RMLs podría corresponder al alternativo o M2

Introduction: Processing and presentation of antigens is involved in the phenomenon of multiple immunological synapses of human Macrophage-Lymphocyte Rosette (MLR) between monocyte-derived macrophages and CD4 + T cells of total leukocyte cultures autologous extracted from blood; here autologous apoptotic neutrophil antigens are presented by the endocytic pathway or via Class II. Class II Compartment (MIIC) has been characterized by B cells and dendritic cells in murine models. Objective: To study the ultrastructural evolution in the spatial organization MIIC in macrophages in the phenomenon of human MLR. Methods: healthy human blood samples were used, anticoagulated with heparin (n = 10) donated by Blood Bank, UNC, anonymous. Autologous leukocyte cultures in TC199 medium (SIGMA, St. Louis, MO). Cell culture samples were taken at 1, 2, 3, 20, 48 and 96 h. MLR technique was applied. The citopreparations underwent processing techniques for ultrastructural study with MET: Zeiss LEO-906E. Results: We observed multivesicular, multilamellar, and tubular bodies in the spatial organization of MIIC throughout the culture time. Tubular structures appeared at 48 h of culture. We conclude that spatial organization of MIIC takes various aspects coinciding with the occurrence of macrophage transformation in culture and its role as antigen presenting cell (APC) in MLRs. Given the autologous antigens presented we postulate that the profile of macrophages in MLRs could correspond to alternative or M2 (AU) .
Descritores: Seres Humanos
Antígeno de Macrófago 1
-Linfócitos
Antígenos CD58
Antígenos
Limites: Seres Humanos
Tipo de Publ: Artigo Clássico
Responsável: CL10.1 - Biblioteca Biomédica


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Machado, Luís dos Ramos
Livramento, José Antonio
Id: lil-154960
Autor: Santiago, Marcelo Fortuna.
Título: Pesquisa de antígenos no LCR e neuroinfecçäo / Antigens investigation in CSF and neuroinfection
Fonte: In: Machado, Luis dos Ramos; Nóbrega, José Paulo Smith; Livramento, José Antonio; Spina França Netto, Antonio. Neuroinfecçäo 94. Säo Paulo, Hospital das Clínicas da Faculdade de Medicina da Universidade de Säo Paulo. Clínica Neurológica, 1994. p.80-80.
Idioma: pt.
Conferência: Apresentado em: Simpósio Neuroinfecçäo-94, Säo Paulo, 18-19 mar. 1994.
Descritores: Antígenos/líquido cefalorraquidiano
Doenças do Sistema Nervoso Central/diagnóstico
Infecção/diagnóstico
-Antígenos de Bactérias/líquido cefalorraquidiano
Antígenos HIV/líquido cefalorraquidiano
Reações Antígeno-Anticorpo
Limites: Seres Humanos
Responsável: BR1.1 - BIREME
BR1.1; 2617.15; BR599.1; 10001009554, AG


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Martins, Marina Lobato
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Id: lil-764211
Autor: Schmidt, Luciana Cayres; Castilho, Lilian; Vieira, Otavio Vinicius Neves; Sippert, Emília; Gaspardi, Ane Caroline; Martins, Marina Lobato; Malta, Maria Clara Fernandes da Silva.
Título: Impact of a confirmatory RhD test on the correct serologic typing of blood donors
Fonte: Rev. bras. hematol. hemoter;37(5):302-305, Sept.-Oct. 2015. ilus.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de Minas Gerais; . Fundação de Amparo à Pesquisa do Estado de Minas Gerais; . Fundação de Amparo à Pesquisa do Estado de Minas Gerais; . Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: BACKGROUND: The RHD gene is highly polymorphic, which results in a large number of RhD variant phenotypes. Discrepancies in RhD typing are still a problem in blood banks and increase the risk of alloimmunization. In this study, the RhD typing strategy at a blood bank in Brazil was evaluated.METHODS: One-hundred and fifty-two samples typed as RhD negative and C or E positive by routine tests (automated system and indirect antiglobulin test using the tube technique) were reevaluated for RhD status by three methods. The method with the best performance was implemented and evaluated for a period of one year (n = 4897 samples). Samples that were D positive exclusively in the confirmatory test were submitted to molecular analysis.RESULTS: The gel test for indirect antiglobulin testing with anti-D immunoglobulin G (clone ESD1) presented the best results. Seventy samples (1.43%) previously typed as RhD negative showed reactivity in the gel test for indirect antiglobulin testing and were reclassified as D positive. D variants that may cause alloimmunization, such as weak D type 2 and partial DVI, were detected.CONCLUSION: The confirmatory RhD test using the gel test for indirect antiglobulin testing represents a breakthrough in transfusion safety in this blood center. Our results emphasize the importance of assessing the blood group typing strategy in blood banks.
Descritores: Sistema do Grupo Sanguíneo ABO
Sorotipagem
Transfusão de Eritrócitos
Biologia Molecular
Antígenos
Limites: Seres Humanos
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Tendler, Miriam
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Id: lil-623676
Autor: Tendler, Miriam.
Título: Schistosoma mansoni: protective antigens
Fonte: Mem. Inst. Oswaldo Cruz;82(supl.4):125-128, 1987.
Idioma: en.
Conferência: Apresentado em: International Symposium on Schistosomiasis, Apresentado em: Reunião Nacional de Esquistossomose, 1, Rio de Janeiro, Oct. 25-30, 1987.
Projeto: NIH; . NSF.
Descritores: Schistosoma mansoni/parasitologia
Esquistossomose mansoni/prevenção & controle
Esquistossomose mansoni/terapia
Esquistossomose mansoni/transmissão
-Substâncias Protetoras
Antígenos
Responsável: BR1.1 - BIREME


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Id: lil-623777
Autor: Bach, Marie Anne.
Título: T-cell response to my mycobacterial antigens in lepromatous and tuberculoid leprosy
Fonte: Mem. Inst. Oswaldo Cruz;82(supl.2):153-157, 1987. tab.
Idioma: en.
Conferência: Apresentado em: International Symposium on Immunomodulators: Biology and Therapeutic Applications, Rio de Janeiro, Apr. 26-30, 1987.
Resumo: We showed that a large fraction of lepromatous patients do harbor helper-type circulating T-cells that can be activated in vitro by Mycobacterium leprae. M. leprae and PPD triggered T-cell lines could be then obtained from both tuberculoid and lepromatous patients. The proliferative response of these helper T-cells is predominantly directed against epitopes shared by several species of mycobacteria, in lepromatous patients as well as in tuberculoid patients, but species specific T-cells are also present. When presented in the context of M. leprae, these cross reactive epitopes usually fail to stimulate the T-cell lines of lepromatous patients, because of the contamination of the lines by supressor T-cells actavable by M. leprae. In one lepromatous patient, PPD and M. leprae reactive T-cell lines and clones (of the CD4 phenotype), exhibited a strong cytotoxic activity to autologous target cells coated with antigen: the relevance of this phenomenon to the pathophysiology of lepromatous leprosy remains however unknown.
Descritores: Linfócitos T/imunologia
Hanseníase Tuberculoide/prevenção & controle
Hanseníase Tuberculoide/transmissão
-Antibacterianos
Antígenos
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: lil-623776
Autor: Ivanyi, J.
Título: The use of monoclonal antibodies for the characterization and production of Mycobacterium leprae antigens
Fonte: Mem. Inst. Oswaldo Cruz;82(supl.2):147-151, 1987. tab.
Idioma: en.
Conferência: Apresentado em: International Symposium on Immunomodulators: Biology and Therapeutic Applications, Rio de Janeiro, Apr. 26-30, 1987.
Resumo: Similar immunizations of mice and hybridoma technology were used by several investigators to raise monoclonal antibodies which identified a limited range of epitopes and antigenic molecules. Further studies would have the scope for revealing yet more novel structures. The existing MABs are agreed standard reagents, avaiable to investigators and valuable for several applications. At least six epitopes specific for M. leprae were defined in molecular terms. Monoclonal antibody based immunoassays proved to be invaluable for the screening of recombinant DNA clones and for the topographic study of individual epitopes. Purification of antigens using affinity chromatography requires further development of techniques whilst serology of leprosy is open for clinical and epidemiological evaluation.
Descritores: Anticorpos Monoclonais/uso terapêutico
Mycobacterium leprae
Antígenos/imunologia
-Mycobacterium
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: lil-623790
Autor: Rees, A; Young, D. B; Lamb., J. R.
Título: Recognition of epitopes of infectious antigens
Fonte: Mem. Inst. Oswaldo Cruz;82(supl.1):311-323, Nov. 1987. tab, graf.
Idioma: en.
Conferência: Apresentado em: Meeting on Immunopathology and Pathogenesis of Chagas' Disease, Leishmaniasis and Leprosy, s.l, Nov. 7-8, 1987.
Descritores: Complexo de Reconhecimento de Origem/uso terapêutico
Epitopos/análise
-Antígenos
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: lil-623807
Autor: Ozaki, Luiz Shozo; Mattei, Denise; David, Peter; Mercerau-Puijalon, Odile; Blisnick, Thierry; Silva, Luiz Pereira da.
Título: Antigenic cross-reactivity of plasmodium falciparum antigens expressed in escherichia coli
Fonte: Mem. Inst. Oswaldo Cruz;81(supl.2):89-91, 1986. ilus, tab.
Idioma: en.
Projeto: France. Ministère de l'Industrie et de la Recherche; . Institut Pasteur Production.
Descritores: Plasmodium falciparum
Escherichia coli
Reações Antígeno-Anticorpo/imunologia
-Antígenos
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: lil-623806
Autor: Perrin, Luc; Shaw, Alan.
Título: Protective antigens from erythrocytic stages: recent progress and perspectives for the development of a vaccine
Fonte: Mem. Inst. Oswaldo Cruz;81(supl.2):83-88, 1986. tab.
Idioma: en.
Projeto: Swiss National Research Foundation; . United Nations Development Program for Research and Training in Tropical Diseases.
Descritores: Vacinas/farmacologia
Malária/terapia
Malária/transmissão
-Erythrocebus
Antígenos/uso terapêutico
Limites: Seres Humanos
Responsável: BR1.1 - BIREME



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