Base de dados : LILACS
Pesquisa : D23.050.161 [Categoria DeCS]
Referências encontradas : 329 [refinar]
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Id: lil-789480
Autor: Raveendran, Reena; Wattal, Chand.
Título: Utility of multiplex real-time PCR in the diagnosis of extrapulmonary tuberculosis
Fonte: Braz. j. infect. dis;20(3):235-241, May.-June 2016. tab, graf.
Idioma: en.
Resumo: Abstract Objective The diagnosis of extrapulmonary tuberculosis is still a challenge because of its pauci-bacillary nature. The aim of the study was to evaluate the role of a multiplex PCR assay in the diagnosis of extrapulmonary tuberculosis and to compare the efficiency of two targets, IS6110 and MPB64 to detect Mycobacterium tuberculosis. Methods 150 extrapulmonary samples (61 pus/aspirate, 46 tissue, 32 body fluids, and 11 urine) from clinically suspected cases of tuberculosis were included in the study. All the samples were subjected to direct fluorescent microscopy, TB culture (BacT/ALERT 3D, biomerieux, Durham, North Carolina, USA) and a Multiplexed Tandem PCR targeting two mycobacterial DNA sequences, IS6110 and MPB64. Master-Mix reagents and primers were prepared by AusDiagnostics Pvt. Ltd (Alexandria, New South Wales, Australia). The performance of the assay was assessed using a composite gold standard, which included clinical characteristics, microbiology smear as well as culture, histopathology, cytology, radiology, and response to antitubercular therapy. Results 20.3%, 23.6%, and 45.3% of specimens were positive by smear, culture, and PCR, respectively. The sensitivity and specificity of the multiplex PCR was 91.9% and 88.4%, respectively, using the composite gold standard. Positive and negative predictive values of the PCR were estimated as 85.1% and 93.8%, respectively. Higher positivity was observed with target IS6110 (44.6%) as compared to target MPB64 (18.9%). The sensitivities of IS6110 and MPB64 individual targets were 90.3% and 64.5%, respectively, and specificities were 88.4% and 97.7%, respectively. Conclusion PCR can play an important role in rapid and accurate diagnosis of extrapulmonary tuberculosis. IS6110 alone is an effective target in our part of the country.
Descritores: Tuberculose/diagnóstico
Reação em Cadeia da Polimerase Multiplex
Mycobacterium tuberculosis/genética
Antígenos de Bactérias/genética
-DNA Bacteriano/análise
Amplificação de Genes
Técnicas Bacteriológicas/métodos
Sensibilidade e Especificidade
Meios de Cultura
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-839221
Autor: Aslam, Bilal; Khurshid, Mohsin; Nisar, Muhammad Atif; Muzammil, Saima; Hayat, Sumreen.
Título: Superantigens: procession of frets
Fonte: Braz. j. infect. dis;21(3):357-358, May-June 2017.
Idioma: en.
Descritores: Proteínas de Bactérias/imunologia
Superantígenos/imunologia
-Toxinas Bacterianas/imunologia
Superantígenos/genética
Antígenos de Bactérias/imunologia
Limites: Humanos
Animais
Tipo de Publ: Carta
Responsável: BR1.1 - BIREME


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Id: biblio-888886
Autor: Jee, Babban; Sharma, Pawan; Katoch, Kiran; Joshi, Beenu; Awasthi, Sudhir Kumar.
Título: IL-10 down-regulates the expression of survival associated gene hspX of Mycobacterium tuberculosis in murine macrophage
Fonte: Braz. j. infect. dis;21(4):386-390, July-Aug. 2017. graf.
Idioma: en.
Resumo: Abstract Mycobacterium tuberculosis (MTB) adopts a special survival strategy to overcome the killing mechanism(s) of host immune system. Amongst the many known factors, small heat shock protein 16.3 (sHSP16.3) of MTB encoded by gene hspX has been reported to be critical for the survival of MTB. In the present study, the effect of recombinant murine interferon-gamma (rmIFN-γ) and recombinant murine interleukin-10 (rmIL-10) on the expression of gene hspX of MTB in murine macrophage RAW264.7 has been investigated. By real-time RT-PCR, it was observed that three increasing concentrations (5, 25 and 50 ng/ml) of rmIFN-γ significantly up-regulated the expression of hspX whereas similar concentrations of rmIL-10 (5, 25 and 50 ng/ml) significantly down-regulated the hspX expression. This effect was not only dependent on the concentration of the stimulus but this was time-dependent as well. A contrasting pattern of hspX expression was observed against combinations of two different concentrations of rmIFN-γ and rmIL-10. The study results suggest that rIL-10 mediated down-regulation of hspX expression, in the presence of low concentration of rIFN-γ, could be used as an important strategy to decrease the dormancy of MTB in its host and thus making MTB susceptible to the standard anti-mycobacterial therapy used for treating tuberculosis. However, as these are only preliminary results in the murine cell line model, this hypothesis needs to be first validated in human cell lines and subsequently in animal models mimicking the latent infection using clinical isolates of MTB before considering the development of modified regimens for humans.
Descritores: Proteínas de Bactérias/metabolismo
Regulação Bacteriana da Expressão Gênica/fisiologia
Interferon gama/farmacologia
Interleucina-10/farmacologia
Macrófagos/microbiologia
Mycobacterium tuberculosis/genética
Antígenos de Bactérias/metabolismo
-Fatores de Tempo
Proteínas de Bactérias/genética
Proteínas Recombinantes/farmacologia
Regulação para Baixo/efeitos dos fármacos
Relação Dose-Resposta a Droga
Antígenos de Bactérias/genética
Responsável: BR1.1 - BIREME


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Id: biblio-1039204
Autor: Macedo, Alexandre Casimiro de; Cunha Jr, José Evandro; Yaochite, Juliana Navarro Ueda; Tavares, Clodis Maria; Nagao-Dias, Aparecida Tiemi.
Título: Salivary anti-PGL-1 IgM may indicate active transmission of Mycobacterium leprae among young people under 16 years of age
Fonte: Braz. j. infect. dis;21(5):557-561, Sept.-Oct. 2017. tab, graf.
Idioma: en.
Projeto: MCTI/CNPq/MS-SCTIE.
Resumo: Abstract Considering that the main route of Mycobacterium leprae transmission is the upper respiratory tract, detection of salivary antibodies can be a useful tool for diagnosing early infection. The study aimed to analyze salivary anti-PGL-1 IgA and IgM antibodies in 169 children aged 4-16 years old, who lived nearby or inside the house of multibacillary or paucibacillary leprosy patients in two endemic cities in Alagoas State - Brazil. Salivary anti-PGL-1 antibodies were quantified by modified ELISA method. The frequency of contact and clinical form of the index case were significantly associated with salivary antibody levels. High frequency of IgM positivity strongly suggests active transmission of M. leprae in these communities. We suggest in the present work that salivary anti-PGL IgA and IgM are important biomarkers to be used for identifying communities with probable active transmission of M. leprae.
Descritores: Glicolipídeos/análise
Família
Busca de Comunicante
Hanseníase Multibacilar/diagnóstico
Anticorpos Antibacterianos/administração & dosagem
Antígenos de Bactérias/análise
-Saliva/imunologia
Saliva/química
Ensaio de Imunoadsorção Enzimática
Glicolipídeos/imunologia
Hanseníase Paucibacilar/diagnóstico
Mycobacterium leprae/imunologia
Antígenos de Bactérias/imunologia
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Criança
Adolescente
Responsável: BR1.1 - BIREME


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Id: biblio-888922
Autor: Leal, Elida A; Moreira, Josimar D; Nunes, Fernanda F; Souza, Larissa R; Martins, Janaina M; Toledo, Vicente P C; Almeida, Alzira M P; Guimarães, Tania M P.
Título: Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations
Fonte: Braz. j. infect. dis;21(6):620-626, Nov.-Dec. 2017. graf.
Idioma: en.
Resumo: ABSTRACT Objectives: The plague, which is an infectious disease caused by Yersinia pestis, still threatens many populations in several countries. The worldwide increase in human plague cases and the potential use of the bacteria as a biological weapon reinforce the need to study the immunity that is induced by potential vaccine candidates. To determine the immunogenicity of antigenic preparations based on the F1 protein and the total extract from Y. pestis, we assessed the role of these antigens in inducing an immune response. Methods: The immunogenicity of antigenic preparations based on the Y. pestis (YP) total extract and the Y. pestis fraction 1 capsular antigen protein (F1) was determined in Swiss-Webster mice immunized with 40 µg or 20 µg for each preparation. Immunophenotyping was performed by flow cytometry. Results: Animals immunized with the YP total extract did not elicit detectable anti-F1 antibodies (Ab) in the hemaglutination/inhibition (HA/HI) test. Animals immunized with 40 µg or 20 µg of the F1 protein produced anti-F1 Abs, with titres ranging from 1/16 to 1/8132. The average of CD3+-CD4+ and CD3+-CD8+ T cells did not differ significantly between the groups. Neither YP total extract nor F1 protein induced a significant expression of IFN-γ and IL-10 in CD4+ T lymphocytes. In addition, F1 failed to induce IFN-γ expression in CD8+ T cells, unlike the YP total extract. Conclusion: The results showed that F1 protein is not an immunogenic T cell antigen, although the YP total extract (40 µg dose) favoured CD8+ T cell-mediated cellular immunity.
Descritores: Baço/imunologia
Yersinia pestis/imunologia
Vacina contra a Peste/imunologia
Imunogenicidade da Vacina
Antígenos de Bactérias/imunologia
-Peste/prevenção & controle
Baço/citologia
Linfócitos T CD4-Positivos/imunologia
Imunofenotipagem
Interferon gama/imunologia
Interleucina-10/imunologia
Relação CD4-CD8
Linfócitos T CD8-Positivos
Citometria de Fluxo
Imunidade Celular
Limites: Animais
Feminino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-984018
Autor: Chen, Jiazhen; Ruan, Qiaoling; Shen, Yaojie; Wang, Sen; Shao, Lingyun; Zhang, Wenhong.
Título: Assessing and screening for T-cell epitopes from Mycobacterium tuberculosis RD2 proteins for the diagnosis of active tuberculosis
Fonte: Braz. j. infect. dis;22(6):462-471, Nov.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Major Project of the Twelfth Five-Year Plan; . National Natural Science Foundation of China; . Shanghai Science and Technology Commission.
Resumo: ABSTRACT The Region of D eletion 2 (RD2) of Mycobacterium tuberculosis encodes reserved antigens that contribute to bacterial virulence. Among these antigens, Rv1983, Rv1986, Rv1987, and Rv1989c have been shown to be immunodominant in infected cattle; however, their diagnostic utility has not been evaluated in humans.In this study, we screened 87 overlapping synthetic peptides encoded by five RD2 proteins for diagnosing tuberculosis epitopes in 50 active tuberculosis (TB) cases, 31 non-tuberculosis patients and 36 healthy individuals. A pool of promising epitopes was then assessed for their diagnostic value in 233 suspected TB patients using a whole blood IFN-γ release assay.Only 10 peptides were recognized by more than 10% of active tuberculosis patients. The IFN-γ release responses to Rv1986-P9, P15, P16, Rv1988-P4, P11, and Rv1987-P11 were significantly higher in the active TB group than in the control groups (p < 0.05). The whole blood IFN-γ release assay based on these epitopes yielded a sensitivity of 51% and a specificity of 85% in diagnosing active tuberculosis, and the corresponding results using the T-SPOT.TB assay were 76% and 75%, respectively.In conclusion, these results suggest that the six epitopes from the RD2 of M. tuberculosis have potential diagnostic value in TB.
Descritores: Proteínas de Bactérias/imunologia
Tuberculose/diagnóstico
Epitopos de Linfócito T/imunologia
Mycobacterium tuberculosis/imunologia
Antígenos de Bactérias/imunologia
-Proteínas de Bactérias/sangue
Tuberculose/imunologia
Tuberculose Pulmonar/diagnóstico
Tuberculose Pulmonar/imunologia
Estudos de Casos e Controles
Estudos Retrospectivos
Sensibilidade e Especificidade
Epitopos de Linfócito T/sangue
Antígenos de Bactérias/sangue
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-1019551
Autor: Arrym, Mauro Pedromonico; Alves, Paulo César Martins; Castelhano, Mariana Virginello; Mazzola, Taís Nitsch; Lemos, Renata Muller Banzato Pinto de; Zaccariotto, Tânia Regina; Levy, Carlos Emilio; Guimarães, Fernando; Silva, Marcos Tadeu Nolasco da.
Título: Preservation of cytotoxic granule production in response to mycobacterial antigens by T-lymphocytes from vertically HIV-infected Brazilian youth on effective combined antiretroviral therapy
Fonte: Braz. j. infect. dis;23(3):151-159, May-June 2019. tab.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: ABSTRACT Background: HIV infection harms adaptive cellular immunity mechanisms. Long-term virological control by combined antiretroviral therapy (cART) reduces the risk of mycobacterial infections. Thus, we aimed to study cellular responses to mycobacterial antigens in 20 HIV-infected adolescents with at least one year of virological control (HIV-RNA <40 copies/mL) and 20 healthy adolescents. Methods: We evaluated CD8 and γδ T-cell degranulation by measurement of CD107a membrane expression after stimulation with lysates from BCG (10 µg/mL) and H37RA Mycobacterium tuberculosis (Mtb, 10 µg/mL). Immune activation and antigen-presenting ability were also assessed by determination of HLA-DR, CD80, and CD86 markers. Results: TCR γδ T-cell CD107a expression was similar between groups in response to mycobacterial antigens, and lower in the HIV-infected group in response to mitogen. Higher baseline HLA-DR expression and lower mycobacterial-stimulated expression was found within the HIV-infected group. Conclusions: Similar degranulation in stimulated CD8+ and TCR γδ T-cells from HIV-infected adolescents, when compared to healthy controls suggests long-term immunological preservation with immune reconstitution under successful cART. However, differences in HLA-DR expression may represent ongoing inflammation and lower specific responses in HIV-infected youth. These features may be relevant in the context of the precocity and severity of vertically acquired HIV infection.
Descritores: Receptores de Antígenos de Linfócitos T alfa-beta/imunologia
Infecções Oportunistas Relacionadas com a AIDS/imunologia
Linfócitos T CD8-Positivos/imunologia
Fármacos Anti-HIV/uso terapêutico
Mycobacterium tuberculosis/imunologia
Antígenos de Bactérias/imunologia
-Tuberculose/imunologia
Biomarcadores/sangue
Estudos Transversais
Estudos Prospectivos
Imunofenotipagem
Apresentação do Antígeno/imunologia
Transmissão Vertical de Doença Infecciosa
Antígenos de Bactérias/efeitos dos fármacos
Limites: Humanos
Masculino
Feminino
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-1039236
Autor: Dewi, Desak Nyoman Surya Suameitria; Mertaniasih, Ni Made; Soedarsono,; Ozeki, Yuriko; Artama, Wayan Tunas; Fihiruddin,; Niki, Mamiko; Tateishi, Yoshitaka; Ato, Manabu; Matsumoto, Sohkichi.
Título: Characteristic profile of antibody responses to PPD, ESAT-6, and CFP-10 of Mycobacterium tuberculosis in pulmonary tuberculosis suspected cases in Surabaya, Indonesia
Fonte: Braz. j. infect. dis;23(4):246-253, July-Aug. 2019. tab, graf.
Idioma: en.
Projeto: Ministry of Research Technology and the High Education Republic of Indonesia; . Japan Agency for Medical Research and Development.
Resumo: Abstract Accurate and rapid diagnostic tools are important aspects of managing tuberculosis (TB) cases appropriately. However, the sensitivity and specificity of diagnostic kits based on immune response such as the tuberculin skin test (TST) and interferon gamma release assay (IGRA) are still debated. Thus, the exploration and assessment of specific biomarker-targeted antibodies are needed for the development of an accurate and rapid diagnostic tool. The present study was conducted in patients with a respiratory problem suspected to be TB at Dr. Soetomo Hospital, Surabaya, Indonesia. Among 102 patients tested by GeneXpert and AFB, 59 serum samples were from cases retrospectively determined to have active TB. A total of 102 serum of healthy controls (HC) was also collected. The PPD antigen and the recombinant CFP-10 and ESAT-6 proteins were prepared. Antibody responses against these proteins were evaluated by ELISA. All samples were also screened for the possibility of Mycobacterium avium-intracellulare complex (MAC) infection using Capilla MaC kit. The results showed that TB patients had a significantly higher concentration of IgG antibody in response to PPD than the HC. In addition, the receiver operating characteristic (ROC) curve analysis showed that PPD was acceptable for diagnostic purposes with an AUC value of 0.835 (95% CI 0.770-0.900, p < 0.0001). However, ESAT-6 and CFP-10 had low AUCs, and 32 samples from both groups showed a low concentration of IgA antibody against all antigens. The MAC detection results also showed that the concentration of IgA in the HC group was the highest. The current results indicate that PPD is a better antigen for antibody-based detection of TB than ESAT-6 and CFP-10. Based on the MAC detection assay, 53 people in the HC group were probably infected with rapidly growing nontuberculous mycobacteria (NTM), although antibody response to PPD was low.
Descritores: Proteínas de Bactérias/imunologia
Tuberculina/imunologia
Tuberculose Pulmonar/diagnóstico
Tuberculose Pulmonar/microbiologia
Formação de Anticorpos/imunologia
Mycobacterium tuberculosis/imunologia
Antígenos de Bactérias/imunologia
-Valores de Referência
Tuberculose Pulmonar/sangue
Ensaio de Imunoadsorção Enzimática
Teste Tuberculínico
Estudos de Casos e Controles
Estudos Retrospectivos
Sensibilidade e Especificidade
Estatísticas não Paramétricas
Indonésia
Limites: Humanos
Masculino
Feminino
Criança
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-1094167
Autor: Coppelli, Luis; Díaz, Luis Antonio; Riquelme, Arnoldo; Waeger, Cristian; Rollán, Antonio; Bellolio, Enrique; Araya, Juan Carlos; Villaseca, Miguel Ángel; Villasmil, Miguel; Pérez, Gonzalo; Coppelli, Catalina.
Título: La derivación protocolizada a endoscopía asociada a la detección de Helicobacter pylori mediante antígeno en deposiciones disminuye lista de espera para endoscopía y optimiza la detección de lesiones pre-malignas y cáncer gástrico incipiente / Protocolized referral to endoscopy and Helicobacter pylori detected in stools aimed to decrease endoscopy waiting lists
Fonte: Rev. méd. Chile;147(11):1382-1389, nov. 2019. tab, graf.
Idioma: es.
Resumo: Background Chile has one of the highest mortality rates by gastric cancer (GC) worldwide. Primary prevention of GC and detection of pre-neoplastic and early neoplastic lesions should be a national priority. Aim To assess the impact of the protocolization of endoscopy referral and the use of H. pylori stool antigen test (HPSA) in the management of dyspepsia to decrease the waiting list for endoscopy and increase the detection of gastric pre-neoplastic and early neoplastic lesions. Material and Methods We included all patients referred to the Endoscopy Unit of a regional hospital, from January 2015 to December 2017. We also included patients with known pre-neoplastic lesions and all those with first degree relatives with GC. We implemented protocols for referral of patients with dyspepsia considering the use of HPSA test, prioritizing to endoscopy those with a higher risk of GC. Results A total of 4,641 endoscopies and 2,631 HPSA tests were carried out. After the adoption of these protocols, we observed a 52% decrease in the waiting time for endoscopy. The GC detection rate in this period was 1.8 to 3.1 cases per 100 endoscopies. After the adoption of the protocols, we observed a significant increase in early GC detection rate (from none in 2015 to 13% in 2017, p = 0.03). Conclusions The protocolization of the referral for endoscopy associated with widespread use of HPSA test in the management of patients with dyspepsia, are successful strategies to decrease waiting lists for endoscopy and optimize the detection rate of pre-neoplastic lesions and early GC.
Descritores: Lesões Pré-Cancerosas/diagnóstico
Listas de Espera
Helicobacter pylori/isolamento & purificação
Infecções por Helicobacter/diagnóstico
Dispepsia/diagnóstico
Fezes/microbiologia
Antígenos de Bactérias/análise
-Lesões Pré-Cancerosas/microbiologia
Atenção Primária à Saúde
Encaminhamento e Consulta
Infecções por Helicobacter/complicações
Infecções por Helicobacter/microbiologia
Sensibilidade e Especificidade
Diagnóstico Precoce
Dispepsia/microbiologia
Endoscopia/estatística & dados numéricos
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Labruna, Marcelo Bahia
Almeida, Alzira Maria Paiva de
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Id: biblio-899272
Autor: Rotondano, Tereza Emmanuelle de Farias; Krawczak, Felipe da Silva; Barbosa, Werona de Oliveira; Moraes-Filho, Jonas; Bastos, Fernanda Nieri; Labruna, Marcelo Bahia; Azevedo, Sérgio Santos de; Melo, Marcia Almeida de; Almeida, Alzira Maria Paiva de.
Título: Ehrlichia canis and Rickettsia spp. in dogs from urban areas in Paraiba state, northeastern Brazil / Ehrlichia canis e Rickettsia spp. em cães de áreas urbanas da Paraíba, nordeste do Brasil
Fonte: Rev. bras. parasitol. vet;26(2):211-215, Apr.-June 2017. tab, graf.
Idioma: en.
Resumo: Abstract The aims of our study was to identify Ehrlichia canis and antibodies against Rickettsia spp. belonging to the spotted fever group (SFG) in dogs sampled from Paraiba state, northeastern Brazil. Blood and serum samples collected by convenience from dogs in urban areas of five municipalities were analyzed by real-time PCR for the detection of E. canis DNA and by immunofluorescence assay test (IFAT) for the identification of antibodies against Rickettsia rickettsii, R. felis, R. parkeri, R. amblyommii and R. rhipicephali antigens. E. canis DNA was detected in 8.9% (64/719) of the blood samples, whereas 5.63% (43/763) of the serum samples were positive for at least one of the Rickettsia antigens tested by IFAT. This study showed for the first time the occurrence of E. canis and suggested the circulation of SFG Rickettsia in dogs in the study region of Paraiba state, northeastern Brazil.

Resumo Os objetivos do nosso estudo foram identificar Ehrlichia canis e anticorpos contra Rickettsia spp. pertencentes ao Grupo da Febre Maculosa (GFM) em cães amostrados no estado da Paraíba, nordeste do Brasil. As amostras de sangue e soro, coletados por conveniência, de cães em áreas urbanas de cinco municípios foram analisadas por PCR em tempo real para a detecção de DNA de E. canis e pela Reação de Imunofluorescência Indireta (RIFI) para identificação de anticorpos contra Rickettsia rickettsii, R. felis, R. parkeri, R. amblyommii e R. rhipicephali. O DNA de E. canis foi detectado em 8,9% (64/719) das amostras de sangue, enquanto que 5,63% (43/763) das amostras de soro foram positivas para pelo menos um dos antígenos de Rickettsia testados por RIFI. Este estudo mostrou pela primeira vez a ocorrência de E. canis e sugere a circulação de Rickettsia do GFM em cães na região em estudo do estado da Paraíba, Nordeste do Brasil.
Descritores: Rickettsia/imunologia
Ehrlichia canis/isolamento & purificação
-Rickettsia rickettsii/imunologia
Brasil
DNA Bacteriano/sangue
Ehrlichia canis/genética
Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/sangue
Limites: Animais
Cães
Responsável: BR1.1 - BIREME



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