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Pesquisa : D23.050.161 [Categoria DeCS]
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Id: biblio-841767
Autor: Rizzi, Caroline; Peiter, Ana Carolina; Oliveira, Thaís Larré; Seixas Neto, Amilton Clair Pinto; Leal, Karen Silva; Hartwig, Daiane Drawanz; Seixas, Fabiana Kommling; Borsuk, Sibele; Dellagostin, Odir Antônio.
Título: Stable expression of Mycobacterium bovis antigen 85B in auxotrophic M. bovis bacillus Calmette-Guérin
Fonte: Mem. Inst. Oswaldo Cruz;112(2):123-130, Feb. 2017. tab, graf.
Idioma: en.
Projeto: BiotechSur; . CNPq.
Resumo: BACKGROUND Bovine tuberculosis (TB) is a zoonotic disease caused by Mycobacterium bovis, responsible for causing major losses in livestock. A cost effective alternative to control the disease could be herd vaccination. The bacillus Calmette-Guérin (BCG) vaccine has a limited efficacy against bovine TB, but can improved by over-expression of protective antigens. The M. bovis antigen 85B demonstrates ability to induce protective immune response against bovine TB in animal models. However, current systems for the construction of recombinant BCG expressing multiple copies of the gene result in strains of low genetic stability that rapidly lose the plasmid in vivo. Employing antibiotic resistance as selective markers, these systems also compromise vaccine safety. We previously reported the construction of a stable BCG expression system using auxotrophic complementation as a selectable marker. OBJECTIVES The fundamental aim of this study was to construct strains of M. bovis BCG Pasteur and the auxotrophic M. bovis BCG ΔleuD expressing Ag85B and determine their stability in vivo. METHODS Employing the auxotrophic system, we constructed rBCG strains that expressed M. bovis Ag85B and compared their stability with a conventional BCG strain in mice. Stability was measured in terms of bacterial growth on the selective medium and retention of antigen expression. FINDINGS The auxotrophic complementation system was highly stable after 18 weeks, even during in vivo growth, as the selective pressure and expression of antigen were maintained comparing to the conventional vector. MAIN CONCLUSION The Ag85B continuous expression within the host may generate a stronger and long-lasting immune response compared to conventional systems.
Descritores: Plasmídeos/genética
Plasmídeos/imunologia
Vacina BCG/genética
Vacina BCG/imunologia
Vetores Genéticos/imunologia
Mycobacterium bovis/genética
Mycobacterium bovis/imunologia
Antígenos de Bactérias/imunologia
Antígenos de Bactérias/metabolismo
-Escherichia coli/genética
Vetores Genéticos
Camundongos Endogâmicos BALB C
Limites: Animais
Feminino
Camundongos
Responsável: BR1.1 - BIREME


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Id: biblio-889161
Autor: Mirhosseini, Seyed Ali; Fooladi, Abbas Ali Imani; Amani, Jafar; Sedighian, Hamid.
Título: Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis
Fonte: Braz. j. microbiol;48(4):774-781, Oct.-Dec. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Food-borne diseases, caused by the pathogenic bacteria, are highly prevalent in the world. Salmonella is one of the most important bacterial genera responsible for this. Salmonella Enteritidis (SE) is one of the non-typhoid Salmonellae that can be transmitted to human from poultry products, water, and contaminated food. In recent years, new and rapid detection methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) have been developed. In this study, recombinant FliC (rFliC) was produced to be used as an antigen. The immunization was conducted in mice with the purified recombinant FliC (rFliC). The mice were subcutaneously immunized with rFliC and elicited significant rFliC specific serum IgG antibodies. An indirect ELISA system was established for the detection of Salmonella Enteritidis. Our results confirmed that the recombinant flagellin can be one of the excellent indicators for the detection of Salmonella Enteritidis.
Descritores: Ensaio de Imunoadsorção Enzimática/métodos
Flagelina/análise
Salmonella enteritidis/isolamento & purificação
-Anticorpos Antibacterianos/análise
Anticorpos Antibacterianos/imunologia
Antígenos de Bactérias/análise
Antígenos de Bactérias/imunologia
Proteínas de Bactérias/análise
Proteínas de Bactérias/genética
Proteínas de Bactérias/imunologia
Flagelina/genética
Flagelina/imunologia
Camundongos Endogâmicos BALB C
Salmonella enteritidis/genética
Salmonella enteritidis/imunologia
Limites: Seres Humanos
Animais
Camundongos
Responsável: BR1.1 - BIREME


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Id: biblio-865848
Autor: Sipert, Carla Renata.
Título: Estudo in vitro da produção de quimiocinas e pró-colágeno I por fibroblastos de gengiva, ligamento periodontal e polpa dental humanos / In vitro study of chemokines and procollagen I by human gingival, periodontal ligament and dental pulp fibroblasts.
Fonte: Bauru; s.n; 2011. 98 p. ilus, tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Odontologia de Bauru para obtenção do grau de Doutor.
Resumo: Fibroblastos são as células mais numerosas encontradas nos tecidos orais como gengiva, ligamento periodontal e polpa dental. Além de exercerem função estrutural, estas células também desempenham papel importante na resposta imune destes tecidos através do reconhecimento de antígenos e produção de mediadores inflamatórios e citocinas. Evidências apontam ainda para o fato de que fibroblastos não constituem um grupo único de células. Sendo assim, os objetivos deste estudo foram: (I) avaliar a produção diferencial de fibroblastos de gengiva, ligamento periodontal e polpa dental de dentes permanentes e decíduos quanto à produção das quimiocinas CCL3 e CXCL12; (II) avaliar a produção de pró-colágeno I pelas células de polpa e (III) avaliar a expressão diferencial dos fibroblastos quanto a microRNAs. Dentes recentemente extraídos (terceiros molares hígidos) e fragmentos de gengivas saudáveis de três pacientes adultos foram obtidos no Laboratório de Farmacologia e Fisiologia Clínica da Faculdade de Odontologia de Bauru. Caninos decíduos de dois pacientes com indicação para extração por motivos ortodônticos foram obtidos na Clínica de Odontopediatria da mesma unidade. Culturas primárias de fibroblastos de gengiva (n=3), ligamento periodontal (n=3) e polpa de dente permanente (n=3) e polpa dental de dente decíduo (n=2) foram estabelecidas a partir de tecidos humanos por meio de técnica de explant. Após a quarta passagem, a produção de CCL3 e de CXCL12 foi avaliada após estímulo com concentrações crescentes (0 10 µg/mL) de ácido lipoteicóico de Enterococcus faecalis (EfLTA), lipopolissacarídeo de Porphyromonas gingivalis (PgLPS) ou LPS de Escherichia coli (EcLPS) por ELISA após 1, 6 e 24 h. O RNAm para as quimiocinas no grupo estimulado com EcLPS por 24 h foi avaliado por transcrição reversa seguida de reação em cadeia da polimerase quantitativa. A produção de pró-colágeno I por células de polpa estimuladas com EfLTA e PgLPS foi avaliada por imunofluorescência. O perfil...

Fibroblasts are the dominant cells within oral tissues such as gingiva, periodontal ligament and dental pulp. Besides the architectural maintenance of the connective tissues, fibroblasts are also involved in connective tissue immune response through antigen recognition and production of inflammatory mediators and cytokines. Recent studies also demonstrated that fibroblasts do not constitute a unique group of cells. Taken this togeter, the objectives of the present study were: (I) to evaluate the production of the chemokines CCL3 and CXCL12 by human gingival, periodontal ligament as well as permanent and deciduous dental pulp fibroblasts; (II) to evaluate the production of procollagen I by dental pulp fibroblasts and (III) to evaluate the differential pattern of expression of microRNAs by the oral fibroblasts. Recently extracted teeth (non-carious third molars) and fragments of healthy gingiva from three adults were obtained at the Laboratory for Clinical Pharmacology and Physiology at Dental School of Bauru. Deciduous canines from two patients with orthodontic indication for extraction were obtained at Pediatrics Clinics of Dental School of Bauru. Primary cultures of fibroblasts from gingiva (n=3), periodontal ligament (n=3) as well as permanent pulp (n=3) and deciduous pulp (n=2) were established through an explant technique. After the fourth passage, fibroblasts were challenged with increasing concentrations (0 10 µg/mL) of Enterococcus faecalis lipoteichoic acid (EfLTA), Porphyromonas gingivalis lipopolysaccharide (PgLPS) or Escherichia coli LPS (EcLPS) for 1, 6 and 24 h. The chemokines were assessed through ELISA while the mRNA for CCL3 and CXCL12 (EcLPS at 24 h) were assessed through reverse transcription followed by quantitative polymerase chain reaction. The expression of microRNAs was screened through a microarray assay. The production of CCL3 on cell supernatants was detected in all cellular groups, with higher amounts at gingival fibroblasts...
Descritores: Fibroblastos/metabolismo
Gengiva/citologia
Ligamento Periodontal/citologia
Polpa Dentária/citologia
Pró-Colágeno/biossíntese
Quimiocinas/biossíntese
-Análise de Variância
Antígenos de Bactérias/metabolismo
Ensaio de Imunoadsorção Enzimática
Imunofluorescência
Técnicas In Vitro
Reação em Cadeia da Polimerase
Limites: Seres Humanos
Responsável: BR28.1 - Serviço de Biblioteca e Documentação Professor Doutor Antônio Gabriel Atta


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Id: biblio-839507
Autor: BERTOLDO, Barbara Bellocchio; SILVA, Camilla Beatriz da; RODRIGUES, Denise Bertulucci Rocha; GERALDO-MARTINS, Vinicius Rangel; FERRIANI, Virginia Paes Leme; NOGUEIRA, Ruchele Dias.
Título: Comparisons of IgA response in saliva and colostrum against oral streptococci species
Fonte: Braz. oral res. (Online);31:e39, 2017. tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Pesquisa.
Resumo: Abstract The present study compared IgA specificity against oral streptococci in colostrum and saliva samples. Sixty-two mother-and-child pairs were included; samples of colostrum (C) and saliva (MS) were collected from the mothers and saliva samples were collected from babies (BS). The specificity of IgA against Streptococcus mutans and S. mitis were analyzed by western blot. Only 30% of babies’ samples presented IgA reactivity to S. mutans, while 74 and 80% of MS and C, respectively, presented this response. IgA reactivity to S. mutans virulence antigens (Ag I/II, Gtf and GbpB) in positive samples showed differences between samples for Gtf and especially for GbpB (p < 0.05), but responses to Ag I/II were similar (p > 0.05). The positive response of Gtf-reactive IgA was different between C (90%) and MS (58%) samples (p < 0.05), but did not differ from BS (p > 0.05). GbpB was the least detected, with 48 and 26% of C and MS, and only 5% of BS samples presenting reactivity (p > 0.05). Eight percent of MS and C samples presented identical bands to SM in the same time-point. In conclusion, the differences of IgA response found between C and MS can be due to the different ways of stimulation, proliferation and transportation of IgA in those secretions. The colostrum has high levels of IgA against S. mutans virulence antigens, which could affect the installation and accumulation process of S. mutans, mainly by supplying anti-GbpB IgA to the neonate.
Descritores: Colostro/imunologia
Imunoglobulina A Secretora/análise
Imunoglobulina A Secretora/imunologia
Saliva/imunologia
Streptococcus mitis/imunologia
Streptococcus mutans/imunologia
-Análise de Variância
Formação de Anticorpos/imunologia
Antígenos de Bactérias/análise
Antígenos de Bactérias/imunologia
Proteínas de Bactérias/análise
Proteínas de Bactérias/imunologia
Western Blotting
Colostro/microbiologia
Ensaio de Imunoadsorção Enzimática
Glucosiltransferases/análise
Glucosiltransferases/imunologia
Glicoproteínas/análise
Glicoproteínas/imunologia
Mães
Saliva/microbiologia
Virulência
Limites: Seres Humanos
Feminino
Recém-Nascido
Responsável: BR1.1 - BIREME


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Id: lil-794728
Autor: Gallo, Juliana Failde; Pinhata, Juliana Maira Watanabe; Chimara, Erica; Gonçalves, Maria Gisele; Fukasawa, Lucila Okuyama; Oliveira, Rosangela Siqueira de.
Título: Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting
Fonte: Mem. Inst. Oswaldo Cruz;111(9):545-550, Sept. 2016. tab.
Idioma: en.
Resumo: Abstract Brazil is one of the high burden countries for tuberculosis, and a rapid diagnosis is essential for effective control of the disease. In the present study, an in-house real-time polymerase chain reaction (PCR) assay targeting the mpt64 gene for identification of Mycobacterium tuberculosis complex isolates was evaluated under routine diagnosis conditions in a reference laboratory. From May 2011 to July 2012, 1,520 isolates of mycobacteria were prospectively submitted for phenotypic and/or PRA-hsp65 identification and to real-time PCR. The mpt64 real-time PCR showed 99.7% sensitivity and 96% specificity and detected 79.4% of the cases missed by phenotypic and PRA-hsp65 identification. The in-house real-time PCR assay showed high sensitivity and specificity and was successfully implemented in the routine diagnosis of tuberculosis in a reference laboratory from a high burden setting.
Descritores: Antígenos de Bactérias/genética
Mycobacterium tuberculosis/genética
Tuberculose/diagnóstico
-Reação em Cadeia da Polimerase em Tempo Real
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Fatores de Tempo
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: lil-779787
Autor: Barcellos, C. C. C.; Fonseca, A. B. M.; Souza, M. C. L.; Franco, R. M.; Mesquita, E. F. M..
Título: Influência da aplicação de irradiação por feixe de elétrons na qualidade microbiológica de filés de corvina (Micropogonias furnieri) refrigerados / Influence of irradiation application for electron beam on the microbiological quality of chilled croaker (Micropogonias furnieri) fillets
Fonte: Arq. bras. med. vet. zootec;68(2):535-542, mar.-abr. 2016. graf.
Idioma: pt.
Resumo: A adequada manipulação do pescado desde sua captura até seu processamento tecnológico, além da manutenção das condições higiênico-sanitárias, o que inclui a qualidade da água utilizada na cadeia, influencia na carga microbiana inicial apresentada. A fim de retardar o processo de deterioração, diminuir as perdas e os riscos iminentes à saúde coletiva, como a propagação de agentes etiológicos de doenças alimentares, são empregados diferentes métodos de conservação. Os feixes de elétrons são utilizados em vários países e levam à destruição dos microrganismos por alterações em suas estruturas, as quais ocorrem pela remoção de elétrons de seus átomos. Objetivou-se, no presente trabalho, contribuir para a avaliação da eficiência da irradiação por feixe de elétrons na qualidade microbiológica de filés de corvina (M. furnieri) refrigerados, desembarcados no município de Niterói - RJ, Brasil. Foram realizadas contagem de bactérias heterotróficas aeróbias mesófilas, de bactérias heterotróficas aeróbias psicrotróficas e enumeração de Enterococcus spp. e, posteriormente, comparadas as amostras do grupo controle com as dos grupos irradiados a doses de 0,7 e 1,0kGy. Os peixes inteiros foram adquiridos no cais de Itaipu, filetados no mercado, embalados a vácuo e mantidos a ±4°C. Embora não tenha sido encontrada diferença estatisticamente significativa entre os três grupos (P>0,05) em nenhuma das análises, concluiu-se que o processamento utilizado foi eficaz na redução do crescimento das três bactérias pesquisadas no dia zero de ambos os grupos irradiados.

The proper handling of fish from capture to technological processing, as well as maintenance of sanitary conditions and the quality of water, can influence the initial microbial load presented. In order to slow down the deterioration process, the reduction of losses and eminent risk to public health as the spread of etiological agents of foodborne illness are different methods used for storage. Electron beams are used in several countries and lead to the destruction of microorganisms by changes in their structures, which occur by removing electrons from their atoms. The objective in the present study contributes to the evaluation of electron beam irradiation efficiency in microbiological quality of chilled croaker fillets (M. furnieri), landed in Niterói - RJ, Brazil. Were performed Bacteria Count Heterotrophic aerobic mesophilic, psychrotrophic bacteria Heterotrophic Aerobic and enumeration of Enterococcus spp. and subsequently compared to the control group of samples with the groups irradiated at doses of 0.7 and 1.0kGy. Whole fish were purchased from the Itaipu pier, threaded on the market, vacuum packed and kept at ±4°C. Although there was no statistically significant difference among the three groups (P>0.05) in any of the analyzes, it was concluded that the application of this technology was effective in reducing the growth of the three bacteria surveyed on day zero of both irradiated groups.
Descritores: Conservação de Alimentos/métodos
Irradiação de Alimentos
Noxas/efeitos da radiação
Peixes/microbiologia
-Antígenos de Bactérias/efeitos da radiação
Carga Bacteriana/veterinária
Análise Microbiológica
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-778999
Autor: Oliveira, Fábio Muniz de; Trentini, Monalisa Martins; Junqueira-Kipnis, Ana Paula; Kipnis, André.
Título: The mc2-CMX vaccine induces an enhanced immune response against Mycobacterium tuberculosis compared to Bacillus Calmette-Guérin but with similar lung inflammatory effects
Fonte: Mem. Inst. Oswaldo Cruz;111(4):223-231, Apr. 2016. graf.
Idioma: en.
Resumo: Although the attenuated Mycobacterium bovis Bacillus Calmette-Guérin (BCG) vaccine has been used since 1921, tuberculosis (TB) control still proceeds at a slow pace. The main reason is the variable efficacy of BCG protection against TB among adults, which ranges from 0-80%. Subsequently, the mc2-CMX vaccine was developed with promising results. Nonetheless, this recombinant vaccine needs to be compared to the standard BCG vaccine. The objective of this study was to evaluate the immune response induced by mc2-CMX and compare it to the response generated by BCG. BALB/c mice were immunised with both vaccines and challenged withMycobacterium tuberculosis (Mtb). The immune and inflammatory responses were evaluated by ELISA, flow cytometry, and histopathology. Mice vaccinated with mc2-CMX and challenged with Mtb induced an increase in the IgG1 and IgG2 levels against CMX as well as recalled specific CD4+ T-cells that produced T-helper 1 cytokines in the lungs and spleen compared with BCG vaccinated and challenged mice. Both vaccines reduced the lung inflammatory pathology induced by the Mtb infection. The mc2-CMX vaccine induces a humoral and cellular response that is superior to BCG and is efficiently recalled after challenge with Mtb, although both vaccines induced similar inflammatory reductions.
Descritores: Vacina BCG/imunologia
Mycobacterium bovis/imunologia
Mycobacterium smegmatis/imunologia
Mycobacterium tuberculosis/imunologia
Tuberculose Pulmonar/imunologia
-Antígenos de Bactérias
Modelos Animais de Doenças
Pulmão/efeitos dos fármacos
Camundongos
Camundongos Endogâmicos BALB C
Tuberculose Pulmonar/patologia
Tuberculose Pulmonar/prevenção & controle
Vacinas Sintéticas/imunologia
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-775116
Autor: Calik, Zeki; Karamese, Murat; Acar, Osman; Karamese, Selina Aksak; Dicle, Yalcin; Albayrak, Fatih; Can, Serpil; Guvendi, Bulent; Turgut, Alpgiray; Cicek, Mustafa; Yazgi, Halil.
Título: Investigation of Helicobacter pylori antigen in stool samples of patients with upper gastrointestinal complaints
Fonte: Braz. j. microbiol;47(1):167-171, Jan.-Mar. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Helicobacter pylori infection is usually acquired in early childhood and it can persist throughout life without antibiotic treatment. This study aimed to compare the accuracy of the noninvasive H. pylori Stool Antigen Test-applied on the stool samples with the invasive gold standart Rapid Urease Test-applied on the gastric biopy samples of patients with upper gastrointestinal complaints. After endoscopy, biopsy and stool specimens were taken in 122 patients. The infection was detected with rapid urease test which is accepted as gold standart test. Rapid, one-step H. pylori card test was applied to all patients stool specimens. In this study 106 of the 122 patients (86.8%) were positive for H. pylori infection, while 16 of the 122 patients (13.2%) were negative. H. pylori card test was negative in 13 of the 16 patients and was positive in 98 of the 106. The sensitivity, specifity, positive and negative predictive values were 92.45%, 81.25%, 97.02%, and 61.90%, respectively. H. pylori card test is rapid, easy, noninvasive and inexpensive methods for detection H. pylori infection. This test showed high sensitivity and specificity. Additionally, it may be a good alternative to invasive tests for the detection of H. pylori infections especially in children.
Descritores: Antígenos de Bactérias/análise
Fezes/microbiologia
Gastroenteropatias/diagnóstico
Helicobacter pylori/isolamento & purificação
-Fezes/química
Valor Preditivo dos Testes
Sensibilidade e Especificidade
Limites: Seres Humanos
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-771926
Autor: VINAGRE, Igor Dias Ferreira; QUEIROZ, André Lima de; SILVA JÚNIOR, Mário Ribeiro da; VINAGRE, Ruth Maria Dias Ferreira; MARTINS, Luisa Caricio.
Título: HELICOBACTER PYLORI INFECTION IN PATIENTS WITH DIFFERENT GASTROINTESTINAL DISEASES FROM NORTHERN BRAZIL
Fonte: Arq. gastroenterol;52(4):266-271, Oct.-Dec. 2015. tab.
Idioma: en.
Resumo: Background - The mechanisms whereby Helicobacter pylori produces different pathological manifestations in the stomach and duodenum are not fully understood. Considering the geographic diversity in the prevalence of virulence factors of this microorganism and their association with the development of different diseases, the search for pathogenicity markers such as CagA and VacA alleles by molecular techniques has intensified. Objectives - To investigate the presence of H. pylori infection and the frequency of different genotypes of this bacterium in patients with gastrointestinal diseases from Northern Brazil, and to establish their association with the histopathological findings. Methods - In a prospective study, samples were collected from 554 patients with different gastrointestinal diseases (gastritis, duodenal ulcer, gastric ulcer, and gastric cancer) seen at a referral hospital attending the entire State of Pará, located in the metropolitan region of Belém. Data such as gender and age obtained with an epidemiological questionnaire were analyzed. The presence of H. pylori and the bacterial genotype were investigated by PCR. Gastric biopsies were assessed histologically. Results - The prevalence of H. pylori infection was 91%. Infection was more frequent among patients with gastric ulcer and gastric cancer. In these groups, there was a predominance of men and older patients when compared to the other two groups studied. The predominant bacterial genotype was s1m1cagA+, which was more frequent among patients with gastric ulcer, duodenal ulcer and gastric cancer. A significant association was observed between s1m1cagA+ strains and a higher degree of inflammation, neutrophil activity and development of intestinal metaplasia. Conclusion - The present study demonstrates a high incidence of H. pylori infection in the patients analyzed, especially among those with gastric ulcer and gastric cancer. Virulent s1m1cagA+ strains predominated and were associated with more severe lesions.

Contexto - Os mecanismos pelos quais o H. pylori produz diferentes quadros patológicos no estômago e no duodeno não são totalmente conhecidos. Considerando a diversidade geográfica relacionada à prevalência dos fatores de virulência desse microrganismo e sua associação com o desenvolvimento de diferentes doenças, vem se intensificando a pesquisa de marcadores de patogenicidade, como o CagA e os alelos do VacA por técnicas moleculares. Objetivos - O objetivo desse estudo foi investigar a presença da infecção por H. pylori, e a frequência dos diferentes genótipos dessa bactéria em pacientes com doenças gastrointestinais da nossa região, procurando estabelecer sua associação com os achados histopatológicos. Métodos - Em estudo prospectivo, foram coletadas amostras de 554 pacientes com diferentes doenças gastrointestinais (gastrite, úlcera duodenal, úlcera gástrica e câncer gástrico), atendidos em hospital de referência para todo o Estado do Pará, localizado na região metropolitana de Belém. Foram analisados dados obtidos através de questionário epidemiológico, relacionados ao sexo e faixa etária desses pacientes. A presença do H. pylori e do genótipo bacteriano foi detectada utilizando a PCR. As biopsias gástricas foram avaliadas histologicamente. Resultados - Observou-se uma prevalência de 91% da infecção pelo H. pylori, sendo mais frequente nos portadores de úlcera gástrica e câncer gástrico, nos quais houve predomínio do sexo masculino e a idade foi maior que a dos outros dois grupos estudados. O genótipo bacteriano predominante foi o s1m1cagA positivo, sendo mais frequentes entre os pacientes com úlcera gástrica, úlcera duodenal e câncer gástrico. Houve associação significante das cepas com o genótipo s1m1cagA positivo com maior grau de inflamação, atividade neutrofílica e desenvolvimento de metaplasia intestinal. Conclusão - Nosso estudo demonstra a alta incidência da infecção pelo H. pylori nos pacientes analisados em nosso meio, especialmente em portadores de úlcera e câncer gástricos. As cepas virulentas s1m1cagA+ foram predominantes e estavam associadas a lesões mais graves.
Descritores: Gastroenteropatias/microbiologia
Infecções por Helicobacter/microbiologia
Helicobacter pylori/genética
-Antígenos de Bactérias/genética
Brasil
Proteínas de Bactérias/genética
Genótipo
Infecções por Helicobacter/diagnóstico
Reação em Cadeia da Polimerase
Limites: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Seres Humanos
Masculino
Meia-Idade
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: lil-769835
Autor: Forster, Karine M; Hartwig, Daiane D; Oliveira, Thaís L; Bacelo, Kátia L; Schuch, Rodrigo; Amaral, Marta G; Dellagostin, Odir A.
Título: DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis
Fonte: Mem. Inst. Oswaldo Cruz;110(8):989-995, Dec. 2015. tab, graf.
Idioma: en.
Resumo: Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of theLeptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated the N-terminal region of the leptospiral immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis using immunisation strategies based on DNA prime-protein boost, DNA vaccine, and subunit vaccine. Upon challenge with a virulent strain ofLeptospira interrogans, the prime-boost and DNA vaccine approaches induced significant protection in hamsters, as well as a specific IgG antibody response and sterilising immunity. Although vaccination with recombinant fragment of LigBrep also produced a strong antibody response, it was not immunoprotective. These results highlight the potential of LigBrep as a candidate antigen for an effective vaccine against leptospirosis and emphasise the use of the DNA prime-protein boost as an important strategy for vaccine development.
Descritores: Antígenos de Bactérias/imunologia
Proteínas de Bactérias/imunologia
Vacinas Bacterianas/imunologia
Leptospira/imunologia
Leptospirose/prevenção & controle
Vacinação/métodos
-Adjuvantes Imunológicos
Biópsia
Cercopithecus aethiops
Sequência Conservada
Ensaio de Imunoadsorção Enzimática
Imunidade Humoral/imunologia
Imunoglobulina A/genética
Imunoglobulina A/imunologia
Imunoglobulina G/imunologia
Imunoglobulinas/genética
Imunoglobulinas/imunologia
Rim/patologia
Leptospirose/imunologia
Pulmão/patologia
Mesocricetus
Análise de Sobrevida
Células Vero
Vacinas de DNA/imunologia
Vacinas Sintéticas/imunologia
Vacinas Sintéticas/microbiologia
Limites: Animais
Cricetinae
Feminino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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