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Aguiar, Maria Cássia Ferreira
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Id: biblio-952056
Autor: Lúcio, Priscilla Suassuna Carneiro; Ribeiro, Daniela Cotta; Aguiar, Maria Cássia Ferreira de; Alves, Pollianna Muniz; Nonaka, Cassiano Francisco Weege; Godoy, Gustavo Pina.
Título: Tumor-associated macrophages (TAMs): clinical-pathological parameters in squamous cell carcinomas of the lower lip
Fonte: Braz. oral res. (Online);30(1):e95, 2016. tab, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; . Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Abstract The objective of this study was to analyze the presence of tumor-associated macrophage (TAM) subpopulations M1 and M2 in squamous cell carcinoma of the lower lip (SCCLL) by immunohistochemitry, and to evaluate the possible role of these subtypes in the development of regional lymph node metastasis and their association with clinical and pathological parameters. Forty-two cases of SCCLL were divided into two groups (21 with and 21 without regional lymph node metastasis). The histopathological grade of malignancy was determined and the material was submitted to double staining with anti-CD68/anti-CD163 and anti-CD68/anti-HLA-DR monoclonal antibodies. The results were analyzed statistically using the Wilcoxon signed-rank and Spearman correlation tests. The M1 and M2 subpopulations were observed in all cases studied. No significant difference was observed between the quantities of M1 and M2 TAMs regarding tumor size (p > 0.05). A significantly larger number of M2 compared to M1 TAMs was observed in tumors without regional lymph node metastasis, tumors in early stages, and low-grade tumors (p < 0.05). No significant difference between the numbers of M1 and M2 TAMs was observed in tumors with regional lymph node metastasis, tumors in advanced stages, and high-grade tumors (p > 0.05). There was a positive weak correlation between M1 and M2 TAMs (r = 0.361; p = 0.019). The results suggest a more important role of M2 TAMs in early stages than advanced stages of lip carcinogenesis. The progression of SCCLL does not seem to be related to an imbalance of macrophage polarization in the microenvironment of these tumors.
Descritores: Neoplasias Labiais/patologia
Carcinoma de Células Escamosas/patologia
Macrófagos/patologia
-Valores de Referência
Imuno-Histoquímica
Antígenos de Diferenciação Mielomonocítica
Antígenos HLA-DR
Antígenos CD
Contagem de Células
Estudos Retrospectivos
Inclusão em Parafina
Receptores de Superfície Celular
Estatísticas não Paramétricas
Gradação de Tumores
Carcinogênese
Metástase Linfática
Estadiamento de Neoplasias
Limites: Humanos
Masculino
Feminino
Responsável: BR1.1 - BIREME


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Id: biblio-1001602
Autor: França, Glória Maria De; Carmo, Andréia Ferreira do; Costa Neto, Hugo; Andrade, Ana Luiza Dias Leite de; Lima, Kenio Costa de; Galvão, Hébel Cavalcanti.
Título: Macrophages subpopulations in chronic periapical lesions according to clinical and morphological aspects
Fonte: Braz. oral res. (Online);33:e047, 2019. tab, graf.
Idioma: en.
Resumo: Abstract: The aim of this study was to evaluate macrophage M1 and M2 subpopulations in radicular cysts (RCs) and periapical granulomas (PGs) and relate them to clinical and morphological aspects. M1 macrophages were evaluated by the percentage of CD68 immunostaining associated with the inflammatory cytokine TNF-α, and M2 macrophages, by its specific CD163 antibody. The CD68+/CD163+ ratio was adopted to distinguish between the two macrophage subpopulations. Clinical, radiographic, symptomatology, treatment, and morphological parameters of lesions were collected and a significance level of p = 0.05 was adopted for statistical analysis. The results showed that the CD68+/CD163+ ratio was higher in the RCs (median = 1.22, p = 0.002), and the highest TNF-α immunostaining scores were found in RCs (p = 0.018); in PGs, the CD68+/CD163+ ratio was lower and associated with a greater CD163+ immunostaining (median = 1.02, p <0.001). The TNF-α in cyst epithelium had a score of 3 in 10 cases and predominance of M1 macrophages by CD68+/CD163+ (median = 2.23). In addition, CD68+ cells had higher percentage of immunostaining in smaller RCs (p = 0.034). Our findings suggest that increased CD68 immunostaining associated with TNF-α cytokine in RCs results in a greater differentiation of the M1 phenotype. The higher CD163 immunostaining in PGs results in greater differentiation of the M2 phenotype. Therefore, the inflammatory state promoted by M1 macrophages is related to growth and progression of RCs; on the other hand, the immunomodulatory state of M2 macrophages is related to maintenance of PGs.
Descritores: Granuloma Periapical/patologia
Cisto Radicular/patologia
Macrófagos/patologia
-Valores de Referência
Imuno-Histoquímica
Antígenos de Diferenciação Mielomonocítica/análise
Antígenos CD/análise
Doença Crônica
Fator de Necrose Tumoral alfa/análise
Receptores de Superfície Celular/análise
Estatísticas não Paramétricas
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Adulto
Responsável: BR1.1 - BIREME


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Id: biblio-1089391
Autor: Daltoe, Felipe Perozzo; Oliveira, Nélio Alessandro Jesus de; Peron, Cibele Nunes; Sharpe, Paul Thomas; Mantesso, Andrea.
Título: Phenotype changes of oral epithelial stem cells after in vitro culture
Fonte: Braz. oral res. (Online);34:e033, 2020. graf.
Idioma: en.
Projeto: FAPESP; . CAPES.
Resumo: Abstract The aim of our study was to isolate populations of keratinocyte stem cells based on the expression of cell surface markers and to investigate whether the culture could affect their phenotype. keratinocytes from human oral mucosa were sorted based on the expression of the epithelial stem cell markers p75NTR and CD71. We also examined the co-expression of other epithelial stem markers such as integrins β1 and α6 and their stem cell-like proprieties in in vitro assays. Three passages after being sorted by MACS, more than 93% of the p75NTR+ve cells lost the expression of p75NTR, while 5.46% of the p75NTR-ve gained it. Within the small population of the p75NTR+ve cells, 88% co-expressed other epithelial stem cell markers such as integrins β1 and α6, while only 28% of p75NTR-ve cells co-expressed these markers. These results were confirmed by sorting cells by FACS. Additionally, when double staining was used for sorting cells, 99% of the p75NTR+veCD71-ve and 33% of the p75NTR-veCD71+ve cells expressed both integrins, but just one week after culture, only 1.74% of the p75NTR+veCD71-ve cells still expressed p75NTR and only 0.32% still expressed CD71. Similar results were obtained when co-culturing p75NTR+ve and p75NTR-ve populations before analysis. Our results suggest that phenotype changes may be part of an intrinsic cellular mechanism to conserve levels of protein expression as they may found in the human body. In addition, in vitro culture may not offer ideal conditions for epithelial stem cell maintenance due to phenotype changes under standard culture conditions.
Descritores: Fenótipo
Células-Tronco/citologia
Queratinócitos/citologia
Técnicas de Cultura de Células/métodos
Células Epiteliais/citologia
Mucosa Bucal/citologia
-Receptores da Transferrina/análise
Biomarcadores/análise
Antígenos CD/análise
Separação Celular/métodos
Reprodutibilidade dos Testes
Receptores de Fator de Crescimento Neural/análise
Citometria de Fluxo/métodos
Proteínas do Tecido Nervoso/análise
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-837532
Autor: Lins, Rodrigo Rego; Oshima, Celina Tizuko Fujiyama; Oliveira, Levindo Alves de; Silva, Marcelo Souza; Mader, Ana Maria Amaral Antonio; Waisberg, Jaques.
Título: Expression of e-cadherin and wnt pathway proteins betacatenin, apc, tcf-4 and survivin in gastric adenocarcinoma: clinical and pathological implication / Expressão da e-caderina e das proteínas da via de sinalização wnt betacatenina, apc, tcf-4 e survivina no adenocarcinoma gástrico: implicações clínica e patológica
Fonte: ABCD arq. bras. cir. dig;29(4):227-231, Oct.-Dec. 2016. tab.
Idioma: en.
Resumo: ABSTRACT Background: Gastric cancer is the fifth most frequent cancer and the third most common cause of cancer-related deaths worldwide.It has been reported that Wnt/ betacatenin pathway is activated in 30-50% of these tumors. However,the deregulation of this pathway has not been fully elucidated. Aim: To determine the expression of E-cadherin, betacatenin, APC, TCF-4 and survivin proteins in gastric adenocarcinoma tissues and correlate with clinical and pathological parameters. Method: Seventy-one patients with gastric adenocarcinoma undergoing gastrectomy were enrolled. The expression of E-cadherin, betacatenin, APC, TCF-4 and survivin proteins was detected by immunohistochemistryand related to the clinical and pathological parameters. Results: The expression rates of E-cadherin in the membrane was 3%; betacatenin in the cytoplasm and nucleus were 23,4% and 3,1% respectively; APC in the cytoplasm was 94,6%; TCF-4 in the nucleus was 19,4%; and survivin in the nucleus 93,9%. The expression rate of E-cadherin was correlated with older patients (p=0,007), while betacatenin with tumors <5 cm (p=0,041) and APC with proximal tumors (p=0,047). Moreover, the expression of TCF-4 was significantly higher in the diffuse type (p=0,017) and T4 tumors (p=0,002). Conclusion: The Wnt/betacatenin is not involved in gastric carcinogenesis. However, the high frequency of survivin allows to suggest that other signaling pathways must be involved in the transformation of gastric tissue.

RESUMO Racional: O câncer gástrico encontra-se entre as principais neoplasias malignas do mundo sendo o quinto mais incidente e o terceiro em relação ao índice de mortalidade. Acredita-se que a via Wnt/betacatenina esteja ativada em 30-50% desses tumores, porém a desregulação dela ainda não está completamente esclarecida. Objetivo: Avaliar a imunoexpressão das proteínas E-caderina, betacatenina, APC, TCF-4 e survivina em tecidos de adenocarcinoma gástrico e correlacioná-las com as variáveis clínicas dos doentes e anatomopatológicas do tumor. Método: Foram coletados os dados clínicos e anatomopatológicos dos prontuários de 71 doentes com adenocarcinoma gástrico submetidos à gastrectomia. O material obtido na operação foi submetido à análise imunoistoquímica e a frequência da expressão de cada proteína pôde ser analisada de acordo com a sua localização na célula e relacionada com as variáveis clinicopatológicas. Resultados: A graduação percentualda expressão e da localização das proteínas foi a seguinte: E-caderina em 3% na membrana; betacatenina em 23,4% no citoplasma e 3,1% no núcleo; APC em 94,6% no citoplasma; TCF-4 em19,4% no núcleo; e survivina em 93,9% no núcleo. Houve relação entre expressão da proteína E-caderina com a idade mais avançada (p=0,007); betacatenina com tumores <5 cm de diâmetro (p=0,041);APC com tumores proximais (p=0,047); e TCF-4 com tipo difuso da classificação de Lauren (p=0,017) e com o grau de penetração tumoral (p=0,002). Conclusão: A via Wnt/betacatenina não está envolvida na carcinogênese gástrica. Porém, a frequência elevada de survivina permite sugerir que outras vias sinalizadoras devam estar envolvidas na transformação do tecido gástrico.
Descritores: Neoplasias Gástricas/metabolismo
Adenocarcinoma/metabolismo
Caderinas/biossíntese
Proteínas Wnt/biossíntese
-Fatores de Transcrição/biossíntese
Antígenos CD
Proteína da Polipose Adenomatosa do Colo/biossíntese
Proteínas Inibidoras de Apoptose/biossíntese
Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos/biossíntese
Via de Sinalização Wnt
Fator de Transcrição 4
Survivina
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Id: biblio-974917
Autor: Wang, Beibei; Wang, Haoyu; Li, Peiquan; Wang, Liangliang; Liu, Hongli; Liu, Jingbo; Wang, Lihua.
Título: Relationships of interleukin-10 with the regulatory T cell ratio and prognosis of cervical cancer patients
Fonte: Clinics;73:e679, 2018. tab, graf.
Idioma: en.
Resumo: OBJECTIVE: This study investigated serum interleukin-10 (IL-10) levels, changes in peripheral blood CD4+CD25+ regulatory T cell (PBCDT) ratios, and the prognosis of cervical cancer (CC) patients. METHODS: Seventy patients with CC composed the observation group, and 70 healthy subjects composed the control group. The PBCDT ratios in the CC patients and healthy subjects were calculated. Serum IL-10 levels were detected with a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). RESULTS: The PBCDT ratio was higher in the patients with active CC [12.16±2.41%] than in the control subjects [6.34±1.05%]. Serum IL-10 levels were higher in the patients with CC [384±106 pg/ml] than in the control subjects [104±50 pg/ml]; the differences in both PBCDT ratio and IL-10 level were statistically significant (p<0.01). Serum IL-10 levels were positively correlated with PBCDT ratios (r=0.375, p<0.05). The 5-year patient survival rate was significantly higher in the low serum IL-10 group (64.2%) than in the high serum IL-10 group (42.8%, p=0.012). CONCLUSIONS: PBCDT ratios and serum IL-10 levels are related to CC activity. These factors are reciprocally related and influence one another, and both are involved in the development and progression of CC. Low IL-10 expression is beneficial regarding the survival of patients with CC.
Descritores: Antígenos CD/sangue
Neoplasias do Colo do Útero/imunologia
Interleucina-10/sangue
Linfócitos T Reguladores/citologia
-Prognóstico
Fatores Socioeconômicos
Ensaio de Imunoadsorção Enzimática
Biomarcadores Tumorais/sangue
Estudos de Casos e Controles
Neoplasias do Colo do Útero/sangue
Neoplasias do Colo do Útero/virologia
Interleucina-10/imunologia
Infecções por Papillomavirus/complicações
Infecções por Papillomavirus/diagnóstico
Estimativa de Kaplan-Meier
Citometria de Fluxo
Estadiamento de Neoplasias
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Responsável: BR1.1 - BIREME


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Id: biblio-1134474
Autor: Yigit, Seyhmus; Ekinci, Cenap.
Título: Changes in Composition of ADAMTS-5 and CD-68 in the Knee Joint Synovial Fluid Cells of Meniscal Tears Patients an Immunohistochemical Study / Cambios en la Composición de ADAMTS-5 y CD-68 en las Células del Líquido Sinovial de la Articulación de la Rodilla en Pacientes con Desgarro de Menisco. Un Estudio Inmunohistoquímico
Fonte: Int. j. morphol;38(6):1539-1543, Dec. 2020. tab, graf.
Idioma: en.
Resumo: SUMMARY: Meniscus tear is an important injury affecting the quality of life. This work is aimed to investigate the activity of CD68 and ADAMTS-5 in cells in synovial fluid in male and female patients with meniscal tear. In this study ,18 male and 22 female patients with meniscal tears were included. Local pain sensation during patients' physical examination, swelling, performing daily activities and difficulty in running-walking complaints were determined. 5 cc synovial fluids were aspirated from the lateral suprapatellar pouch part of the knees with meniscal pain. After routine histological follow-up of the samples, they were embedded in paraffin and sectioned with microtome and 5 micrometer thickness. CD68 and ADAMTS-5 primary antibodies were used for immunohistochemical analysis. Sections were taken and evaluated with a stylish microscope. The distribution of blood cells after meniscus tear was higher in female patients than in male patients. CD68 distribution in female patients appeared higher than in male patients. CD68 expression was high in macrophage cell cytoplasm. ADAMTS-5 expression was higher in female patients in degenerative cells and apoptotic cells. ADAMTS-5 is an important metallo-protein involved in the development of apoptotic signal and extracellular matrix synthesis in patients with ADAMTS-5 meniscus tear, and it may be an important criterion for the treatment developed after injury. CD68 and ADAMTS-5 activity was thought to be one of the important signal pathways that can be identified in the treatment of meniscus tear.

RESUMEN: La rotura del menisco es una lesión importante que afecta la calidad de vida. El objetivo fue investigar la actividad de CD68 y ADAMTS-5 en células del líquido sinovial en pacientes masculinos y femeninos con desgarro meniscal. Se incluyeron 18 pacientes masculinos y 22 femeninos con desgarros meniscales. Se determinó la sensación de dolor local durante el examen físico de los pacientes, la hinchazón, la realización de actividades diarias y la dificultad al correr y caminar. Se aspiraron 5 cc de líquido sinoviale de la parte de la bolsa suprapatelar lateral de las rodillas de los pacientes con dolor meniscal. Después del seguimiento histológico de rutina, las muestras se incluyeron en parafina y se seccionaron con un micrótomo de grosor de 5 micrómetros. Para el análisis inmunohistoquímico se usaron los anticuerpos primarios CD68 y ADAMTS-5. La distribución de las células sanguíneas después del desgarro del menisco fue mayor en pacientes femeninos que en pacientes masculinos. La distribución de CD68 en pacientes femeninos fue más alta que en pacientes masculinos. Además la expresión de CD68 fue alta en el citoplasma de los macrófagos. La expresión de ADAMTS-5 fue mayor en pacientes femeninos en las células degenerativas y células apoptóticas. ADAMTS-5 es una metaloproteína importante en el desarrollo de la señal apoptótica y la síntesis de matriz extracelular en pacientes con rotura de menisco ADAMTS-5, y puede ser un criterio importante para el tratamiento después de la lesión. La actividad de CD68 y ADAMTS-5 era una de las vías de señal importantes que se pueden identificar en el tratamiento de la rotura del menisco.
Descritores: Lesões do Menisco Tibial/metabolismo
Lesões do Menisco Tibial/patologia
Articulação do Joelho/metabolismo
Articulação do Joelho/patologia
-Líquido Sinovial/química
Imuno-Histoquímica
Antígenos CD/análise
Sinoviócitos/metabolismo
Proteína ADAMTS5/análise
Articulação do Joelho/citologia
Limites: Humanos
Masculino
Feminino
Responsável: CL1.1 - Biblioteca Central


  7 / 112 LILACS  
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Id: biblio-1134510
Autor: Salman, Ahmed; El-Ghazouly, Dalia El-Sayed; El-Beltagy, Maha.
Título: Role of ascorbic acid versus silymarin in amelioration of hepatotoxicity induced by acrylamide in adult male albino rats: histological and immunohistochemical study / Rol del ácido ascórbico frente a la silimarina en la mejoría de la hepatotoxicidad inducida por acrilamida en ratas albinas macho adultas: estudio histológico e inmunohistoquímico
Fonte: Int. j. morphol;38(6):1767-1778, Dec. 2020. tab, graf.
Idioma: en.
Resumo: SUMMARY: Acrylamide (ACR) is a cytotoxic and carcinogenic material. It is a product of a Maillard reaction during the cooking of many types of fried fast food, e.g. potato chip fries, and chicken nuggets. ACR has a severe toxic effect on different body organs. This study investigates the hepatotoxic effect of ACR, and the protective effect of ascorbic acid and silymarin. For this purpose, forty adult, male, albino rats were divided into four groups and received the following treatments for fourteen days: Group I: (the control) normal saline; Group II: ACR only; Group III: ACR and ascorbic acid; and Group IV: ACR and silymarin. Under a light microscope, the liver from rats treated with ACR only presented disturbed liver architecture, degenerated hepatocytes, reduced glycogen contents, congested central vein, and increased collagen fibres with areas of fibrosis. Immunohistochemical examination revealed an increased mean number of CD68-, and α-SMA-positive cells. This indicates the presence of large numbers of stellate macrophages (Kupffer cells) and Hepatic stellate cells (HSCs). The combination of ACR with either ascorbic acid or silymarin resulted in less hepatic degeneration, less fibrosis and fewer CD68 and α-SMA positive cells compared to the ACR only group. In conclusion, treatment with silymarin or ascorbic acid along with ACR appears to alleviate ACR-induced hepatotoxicity with more protection in silymarin treated rats.

RESUMEN: La acrilamida (ACR) es un material citotóxico y cancerígeno. Es producto de la reacción de Maillard durante la cocción de muchos tipos de comida rápida y frita, por ejemplo: papas fritas y nuggets de pollo. ACR tiene un efecto tóxico severo en diferentes órganos del cuerpo. Este estudio investigó el efecto hepatotóxico del ACR y el efecto protector del ácido ascórbico y la silimarina. Con este fin, cuarenta ratas albinas machos adultas se dividieron en cuatro grupos y recibieron los siguientes tratamientos durante catorce días: Grupo I (control), solución salina normal; Grupo II, solo ACR; Grupo III, ACR y ácido ascórbico; y Grupo IV, ACR y silimarina. Bajo microscopio óptico, el hígado de ratas tratadas con ACR solo presentó alteración de su arquitectura, entre ellos hepatocitos degenerados, contenido reducido de glucógeno, vena central congestionada y aumento de fibras de colágeno con áreas de fibrosis. El examen inmunohistoquímico reveló un aumento del número medio de células CD68 y α-SMA positivas. Esto indica la presencia de un gran número de macrófagos estrellados (células de Kupffer) y células estrelladas hepáticas (HSC). La combinación de ACR con ácido ascórbico o silimarina resultó en menos degeneración hepática, menos fibrosis y menos células positivas para CD68 y α-SMA en comparación con el grupo de ACR solo. En conclusión, el tratamiento con silimarina o ácido ascórbico junto con ACR parece aliviar la hepatotoxicidad inducida por ACR.
Descritores: Ácido Ascórbico/farmacologia
Silimarina/farmacologia
Acrilamida/toxicidade
Fígado/efeitos dos fármacos
-Imuno-Histoquímica
Antígenos CD/análise
Actinas/análise
Hepatócitos
Células Estreladas do Fígado
Fígado/metabolismo
Fígado/patologia
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


  8 / 112 LILACS  
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Vasconcelos, Pedro Fernando da Costa
Sotto, Mirian Nacagami
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Id: biblio-1057248
Autor: Vitoria, Webster Oliveira; Thomé, Letícia Silva; Kanashiro-Galo, Luciane; Carvalho, Leda Viegas de; Penny, Ricardo; Santos, Washington Luis Conrado; Vasconcelos, Pedro Fernando da Costa; Sotto, Mirian Nacagami; Duarte, Maria Irma Seixas; Quaresma, Juarez Antonio Simões; Pagliari, Carla.
Título: Upregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in renal tissue in severe dengue in humans: Effects on endothelial activation/dysfunction
Fonte: Rev. Soc. Bras. Med. Trop;52:e20180353, 2019. tab, graf.
Idioma: en.
Projeto: MCT/CNPq/FNDCT/CAPES/FAPESPA; . CNPq; . CNPq.
Resumo: Abstract INTRODUCTION: Dengue is an important mosquito-borne disease in tropical and subtropical regions. Adhesion molecules have not been systematically characterized in the renal tissue of patients with severe dengue (SD). The objective of this study was to detect viral antigens in samples from patients that evolved with SD, correlating with the expression of ICAM-1, VCAM-1, VE-cadherin, and E-selectin to contribute to a better understanding of the pathophysiology of SD. METHODS: Kidney specimens from patients with SD were selected according to clinical and laboratorial data and submitted to histological and immunohistochemistry analysis. A semiquantitative evaluation was performed considering positive immunostaining in 20 glomeruli. RESULTS: Viral antigens were mainly detected in distal tubules. The intense immunostaining of VCAM-1 and ICAM-1 was observed. The expression of E-selectin was discrete, and VE-cadherin expression varied from mild to moderate. VCAM-1 was slightly intense in the glomerular capsule; the expression of ICAM-1 was diffuse. E-selectin was diffuse, and VE-cadherin varied from mild to moderate. The most frequent histological findings were glomerular congestion, mild glomerulitis, acute renal injury, and glomerular atrophy. CONCLUSIONS: The results appear to demonstrate an imbalance between vascular endothelial permeability regulating events in renal lesions in SD. The increase in the expression of ICAM-1 and VCAM-1 is an in-situ indicator of higher permeability with a consequent influx of cells favoring the inflammation of the endothelium. These molecules are important in the pathophysiology of the disease and provide the possibility of developing new markers for the evaluation, clinical follow-up, and therapeutic response of patients with SD.
Descritores: Molécula 1 de Adesão Intercelular/fisiologia
Molécula 1 de Adesão de Célula Vascular/fisiologia
Selectina E/fisiologia
Dengue Grave/fisiopatologia
Dengue Grave/sangue
Endotélio/fisiopatologia
-Imuno-Histoquímica
Biomarcadores/sangue
Antígenos CD/fisiologia
Antígenos CD/sangue
Caderinas/fisiologia
Caderinas/sangue
Regulação para Cima
Molécula 1 de Adesão Intercelular/sangue
Progressão da Doença
Molécula 1 de Adesão de Célula Vascular/sangue
Selectina E/sangue
Pessoa de Meia-Idade
Antígenos Virais/sangue
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Criança
Adolescente
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-950823
Autor: Chen, Haide; Li, Yang; Lin, Xijuan; Cui, Di; Cui, Chun; Li, Hui; Xiao, Lei.
Título: Functional disruption of human leukocyte antigen II in human embryonic stem cell
Fonte: Biol. Res;48:1-9, 2015. ilus, graf.
Idioma: en.
Projeto: Ministry of Agriculture; . National Natural Science Foundation of China; . Zhejiang Provincial Natural Science Foundation of China; . Agricultural Variety Breeding Project of Zhejiang Province; . Ministry of Science and Technology of China.
Resumo: BACKGROUND: Theoretically human embryonic stem cells (hESCs) have the capacity to self-renew and differentiate into all human cell types. Therefore, the greatest promise of hESCs-based therapy is to replace the damaged tissues of patients suffering from traumatic or degenerative diseases by the exact same type of cells derived from hESCs. Allo-graft immune rejection is one of the obstacles for hESCs-based clinical applications. Human leukocyte antigen (HLA) II leads to CD4+ T cells-mediated allograft rejection. Hence, we focus on optimizing hESCs for clinic application through gene modification. RESULTS: Transcription activator-like effector nucleases (TALENs) were used to target MHC class II transactivator (CIITA) in hESCs efficiently. CIITA(-/-)hESCs did not show any difference in the differentiation potential and self-renewal capacity. Dendritic cells (DCs) derived from CIITA(-/-)hESCs expressed CD83 and CD86 but without the constitutive HLA II. Fibroblasts derived from CIITA(-/-)hESCs were powerless in IFN-γ inducible expression of HLA II. CONCLUSION: We generated HLA II defected hESCs via deleting CIITA, a master regulator of constitutive and IFN-γ inducible expression of HLA II genes. CIITA(-/-)hESCs can differentiate into tissue cells with non-HLA II expression. It's promising that CIITA(-/-)hESCs-derived cells could be used in cell therapy (e.g., T cells and DCs) and escape the attack of receptors' CD4+ T cells, which are the main effector cells of cellular immunity in allograft.
Descritores: Proteínas Nucleares/genética
Transativadores/genética
Diferenciação Celular/genética
Deleção de Genes
Desoxirribonucleases/metabolismo
Células-Tronco Embrionárias Humanas/metabolismo
-Teratoma
Células Dendríticas/metabolismo
Imunoglobulinas/metabolismo
Imuno-Histoquímica
Glicoproteínas de Membrana/metabolismo
Células Tumorais Cultivadas
Antígenos de Histocompatibilidade Classe II/genética
Antígenos CD/metabolismo
Interferon gama/metabolismo
Camundongos SCID
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Desoxirribonucleases/classificação
Antígeno B7-2/metabolismo
Corpos Embrioides/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Cariótipo
Fibroblastos/metabolismo
Autorrenovação Celular
Células Apresentadoras de Antígenos/metabolismo
Limites: Humanos
Animais
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Alvarenga, Marcia Regina Martins
Texto completo
Id: lil-731290
Autor: Yamashita, Cintia Hitomi; Gaspar, Jaqueline Correia; Amendola, Fernanda; Alvarenga, Márcia Regina Martins; Oliveira, Maria Amélia de Campos.
Título: Social network of family caregivers of disabled and dependent patients / Red social de cuidadores familiares de pacientes con discapacidad y dependencia / Rede social de cuidadores familiares de pacientes com incapacidades e dependência
Fonte: Rev. Esc. Enferm. USP;48(spe):95-101, 08/2014. tab.
Idioma: en.
Resumo: Cross-sectional study that used the Social Network Index and the genogram to assess the social network of 110 family caregivers of dependent patients attended by a Home Care Service in São Paulo, Brazil. Data were analyzed using the test U of Mann-Whitney, Kruskal-Wallis and Spearman correlation. Results were considered statistically significant when p<0,05. Few caregivers participated in activities outside the home and the average number of people they had a bond was 4,4 relatives and 3,6 friends. Caregivers who reported pain and those who had a partner had higher average number of relatives who to trust. The average number of friends was higher in the group that reported use of medication for depression. Total and per capita incomes correlated with the social network. It was found that family members are the primary caregiver’s social network.



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Estudio transversal que utiliza el Índice de la Red Social y el genograma para evaluar la red social de los 110 cuidadores familiares de enfermos dependientes atendidos por un servicio de cuidados en el hogar, en São Paulo. Los datos fueron analizados por las pruebas de Mann-Whitney, Kruskal-Wallis y la correlación de Spearman. Los resultados se consideraron estadísticamente significativos cuando p<0,05. Pocos cuidadores participaban en actividades fuera del hogar y el número promedio de personas con las cuales tenían vínculo fueran 4,4 personas de la familia y 3,6 amigos. Los que informaron dolor en el cuerpo y los que tenían una pareja tenían mayor número medio de familiares en que confiar. El número medio de amigos fue mayor en el grupo que informó el uso de medicación para la depresión. Los ingresos totales y per cápita se correlacionaron con la red social. Se encontró que los miembros de la familia son la principal red social del cuidador.

.

Objetivo Avaliar a rede social de 110 cuidadores familiares de pacientes dependentes atendidos por um Serviço de Assistência Domiciliária no município de São Paulo. Método Estudo transversal, que utilizou o Social Network Index e o genograma. Os dados foram analisados pelos testes U de Mann-Whitney, Kruskal-Wallis e correlação de Spearman. Foram considerados estatisticamente significantes quando p <0,05. Resultados Poucos cuidadores participavam de atividades extradomiciliares e o número médio de pessoas com quem mantinham vínculo era de 4,4 familiares e 3,6 amigos. Cuidadores que referiram dor no corpo e aqueles que possuíam companheiro apresentaram maior número médio de parentes em quem confiar. A média de amigos foi superior no grupo que referiu uso de medicamentos para depressão. As rendas total e per capita mostraram correlação com a rede social. Conclusão Verificou-se que os familiares são a principal rede social do cuidador. .
Descritores: Glicoproteínas de Membrana
Neoplasias Gástricas/genética
-Antígenos CD/genética
Tetraspanina 29
Caspases/genética
Mucosa Gástrica/metabolismo
Perfilação da Expressão Gênica
Genes Supressores de Tumor
Queratinas/genética
Metaloproteinases da Matriz/genética
Análise de Sequência com Séries de Oligonucleotídeos
Receptores do Ácido Retinoico/genética
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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