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Pesquisa : D23.050.301.264.894.095 [Categoria DeCS]
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Id: biblio-915122
Autor: Lopes, Matheus Rodrigues; Pereira, João Kleber Novais; Traina, Fabiola; Campos, Paula de Melo; Machado-Neto, João Agostinho; Saad, Sara Teresinha Olalla; Favaro, Patricia.
Título: IL32 expression in peripheral blood CD3+ cells from myelodysplastic syndromes patients
Fonte: Appl. cancer res;37:1-6, 2017. tab, ilus.
Idioma: en.
Resumo: Background: Myelodysplastic syndromes (MDS) are a heterogeneous group of disorders characterized by ineffective hematopoiesis and risk of leukemia transformation. There is evidence to suggest the participation of immune system deregulation in MDS pathogenesis. Interleukin-32 (IL-32) is a newly described multifunctional cytokine reported as an important mediator in autoimmune and inflammatory disorders. In the present study, we reported the expression of IL32 and IL32 transcript variants (α, ß, γ and δ) in peripheral blood CD3+ cells from healthy controls and MDS patients. Methods: CD3+ cells were isolated by immunomagnetic cell sorting from thirty-nine untreated MDS patients and twenty-nine healthy donors. Gene expression was evaluated by quantitative PCR. For statistical analysis, Mann­Whitney test, Kruskal-Wallis test with Dunns post test and Log-rank (Mantel-Cox) were used, as appropriate. A p value <0.05 was considered statistically significant. Results: IL32 expression and IL32 transcript variants IL32α, IL32ß, IL32γ, and IL32δ, were similar in peripheral blood CD3+ cells from healthy donors and MDS patients. Increased IL-32α expression was an independent predictor for MDS disease progression by univariate and multivariate analysis. Conclusions: We observed that IL32 expression is not differently expressed in CD3+ cells from MDS patients; nevertheless IL32α has a potential role in disease progression (AU)
Descritores: Síndromes Mielodisplásicas
Análise Multivariada
Interleucinas
Complexo CD3
Progressão da Doença
Sistema Imunitário
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Responsável: BR30.1 - Biblioteca


  2 / 6 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-893754
Autor: Furlanetto, Gislaine; Alegretti, Ana Paula; Farias, Mariela Granero; Freitas, Priscila Aparecida Corrêa; Lara, Gustavo Muller; Pedrazzani, Fabiane Spagnol.
Título: Cut-off value for absolute lymphocytes as an alternative for the immunophenotypic analysis of CD3+ T cells in the monitoring of immunosuppressive therapy with thymoglobulin / Valor de corte para linfócitos absolutos como alternativa para análise imunofenotípica de células T CD3+ no monitoramento de terapia imunossupressora com timoglobulina
Fonte: J. bras. nefrol;39(2):181-185, Apr.-June 2017. graf.
Idioma: en.
Resumo: Abstract Introduction: Immunosuppression of T lymphocytes is required for preventing acute rejection after transplantation and for the treatment of chronic autoimmune and inflammatory diseases. The laboratory monitoring for this therapy is the measurement of T cells by immunophenotyping, aiming the target value of less than 20 cells per µL. Objective: To establish a cut-off point for the total number of lymphocytes in the automated blood cell count that reflects less than twenty T cells µL by immunophenotyping. Methods: We studied and evaluated 242 kidney transplant patients that had results of automated blood cell count and quantification of T cells by immunophenotyping technique. The patients were divided into two groups, depending on the T lymphocyte immunophenotyping rates established by lower and higher than 20 cells per µL. After, we evaluated the cut-off point for lymphocytes in the blood cell count with a specificity of 100% to exclude patients with high levels of T lymphocytes. Results: We found that the cut-off point of 70 lymphocytes per µL obtained by automated blood cell count showed 100% of specificity to exclude patients with T-cell counts higher than 20 cells per µL by immunophenotyping. Conclusion: The results found in this study may be helpful to monitor the immunosuppressive therapy in kidney transplant patients in places where a flow cytometer is not available, or when this equipment is not present in the full routine.

Resumo Introdução: A imunossupressão de linfócitos T é necessária para a prevenção da rejeição aguda em transplantes e no tratamento de doenças autoimunes e inflamatórias crônicas. O seu monitoramento laboratorial consiste na quantificação dos linfócitos T realizada pela técnica de imunofenotipagem, na qual o valor preconizado é manter inferior a 20 células/µL. Objetivo: Estabelecer um ponto de corte para o número de linfócitos totais no hemograma automatizado que reflita uma contagem de linfócitos T inferior a 20 células/µL por imunofenotipagem. Métodos: Foram avaliados 242 pacientes transplantados renais que continham resultados do hemograma automatizado e quantificação de linfócitos T por imunofenotipagem. Os pacientes foram divididos em dois grupos, conforme os valores de linfócitos T estabelecidos pela imunofenotipagem: inferiores e superiores a 20 células/µL. A partir disto, foi avaliado o ponto de corte de linfócitos no hemograma com especificidade de 100% para excluir os pacientes com valores elevados de linfócitos T. Resultados: Este estudo evidenciou que o ponto de corte de 70 linfócitos/µL obtidos pelo hemograma automatizado apresentou especificidade de 100% para excluir os pacientes com contagens de linfócitos T superiores a 20 células/µL na imunofenotipagem. Conclusão: Esta pesquisa poderá auxiliar no monitoramento da terapia imunossupressora em pacientes transplantados renais em locais que não possuem um citômetro de fluxo disponível, ou ainda quando este equipamento não se faz presente na rotina integral.
Descritores: Linfócitos T/imunologia
Imunossupressão
Transplante de Rim
Complexo CD3
Imunossupressores/uso terapêutico
Soro Antilinfocitário/uso terapêutico
-Estudos Retrospectivos
Imunofenotipagem/métodos
Monitoramento de Medicamentos
Contagem de Linfócitos
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


  3 / 6 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-949368
Autor: Qian, Yunzhong; Chen, Yi; Wang, Linyang; Tou, Jinfa.
Título: Effects of baicalin on inflammatory reaction, oxidative stress and PKDl and NF-kB protein expressions in rats with severe acute pancreatitis
Fonte: Acta cir. bras;33(7):556-564, July 2018. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To investigate the effects of baicalin on inflammatory reaction, oxidative stress and protein kinase D1 (PKD1) and nuclear factor-kappa B (NF-κB) protein expressions in severe acute pancreatitis (SAP) rats. Methods: Sixty rats were divided into sham operation, model, and low-, medium- and high-dose baicalin group. SAP model was established in later 4 groups. The later 3 groups were injected with 0.1, 0.2 and 0.4 ml/100 g 5% baicalin injection, respectively. At 12 h, the serum SAP related indexes and inflammatory factors, peripheral blood CD3 and γδT cell percentages, wet/dry ratio and pancreas ascites volume, oxidative stress indexes and PKD1 and NF-κB protein expressions in pancreatic tissue were determined. Results: Compared with model group, in high-dose baicalin group the wet/dry ratio and ascites volume, serum amylase level, phospholipase A2 activity, TNF-α, IL-1 and IL-6 levels, and pancreatic malondialdehyde level and PKD1 and NF-κB protein expression were significantly decreased (P < 0.05), and peripheral blood CD3 and γδT cell percentages and pancreatic superoxide dismutase and glutathione peroxidase levels were significantly increased (P < 0.05). Conclusion: Baicalin can resist the inflammatory reaction and oxidative stress, and down-regulate protein kinase D1 and nuclear factor-kappa B protein expressions, thus exerting the protective effects on severe acute pancreatitis in rats.
Descritores: Pancreatite/tratamento farmacológico
Flavonoides/farmacologia
Proteína Quinase C/metabolismo
Anti-Inflamatórios não Esteroides/farmacologia
NF-kappa B/metabolismo
Estresse Oxidativo/efeitos dos fármacos
-Pancreatite/metabolismo
Superóxido Dismutase/efeitos dos fármacos
Proteína Quinase C/efeitos dos fármacos
Distribuição Aleatória
Regulação para Baixo/efeitos dos fármacos
Reprodutibilidade dos Testes
NF-kappa B/efeitos dos fármacos
Interleucina-6/sangue
Interleucina-1/sangue
Fator de Necrose Tumoral alfa/sangue
Resultado do Tratamento
Ratos Sprague-Dawley
Complexo CD3/efeitos dos fármacos
Complexo CD3/sangue
Glutationa Peroxidase/efeitos dos fármacos
Glutationa Peroxidase/metabolismo
Amilases/efeitos dos fármacos
Amilases/sangue
Malondialdeído/metabolismo
Limites: Animais
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  4 / 6 LILACS  
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Texto completo SciELO Brasil
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Id: biblio-983741
Autor: Wang, Shuqin; Fu, Liying; Du, Wenhui; Hu, Jun; Zha, Yongsheng; Wang, Peiguang.
Título: Subsets of T lymphocytes in the lesional skin of pityriasis rosea
Fonte: An. bras. dermatol;94(1):52-55, Jan.-Feb. 2019. tab, graf.
Idioma: en.
Resumo: Abstract: Background: Pityriasis rosea is a common papulosquamous disorder. However, its etiology and pathogenesis remain unclear. Objective: We investigate the types of inflammatory cells infiltrating the lesional skin of pityriasis rosea and demonstrate whether T-cell-mediated immunity is involved in the pathogenesis of this condition or not. Methods: The biopsies were taken from the lesional skin of 35 cases of patients diagnosed with pityriasis rosea. The specimens were prepared in paraffin sections, then submitted to routine immunohistochemistry procedures using monoclonal antibodies directed against CD3, CD4, CD8, CD20 and CD45RO and horseradish peroxidase-labeled goat anti-human antibodies. The positive sections were determined by the ratio and staining intensity of positive inflammatory cells. Results: The mean score of positive CD3, CD4, CD8, and CD45RO staining was respectively 3.74±3.88, 5.67±4.40, 2.94±3.42 and 7.68±4.33 in these pityriasis rosea patients (P<0.001). The percentage of positive staining was 54.29% (19/35), 69.7% (23/33), 40% (14/35) and 79.41% (27/34) (P<0.05). However, the staining of CD20 was negative in all samples. The mean score of CD3 staining in patients with time for remission ≤60 days (4.90±4.21) was higher than that in patients with time for remission >60 days (2.00±2.5) (P<0.05), whereas no statistical difference in the mean score of CD4, CD8 and CD45RO staining was observed. study liMitations: The sample size and the selected monoclonal antibody are limited, so the results reflect only part of the cellular immunity in the pathogenesis of pityriasis rosea. Conclusion: Our findings support a predominantly T-cell mediated immunity in the development of pityriasis rosea.
Descritores: Subpopulações de Linfócitos T/patologia
Pitiríase Rósea/patologia
-Valores de Referência
Coloração e Rotulagem
Fatores de Tempo
Biópsia
Imuno-Histoquímica
Linfócitos T CD4-Positivos/patologia
Subpopulações de Linfócitos T/imunologia
Pitiríase Rósea/imunologia
Antígenos Comuns de Leucócito/análise
Complexo CD3/análise
Linfócitos T CD8-Positivos/patologia
Imunidade Celular
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Adulto Jovem
Responsável: BR1.1 - BIREME


  5 / 6 LILACS  
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Soares, Angela M. V. C
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Id: lil-284229
Autor: Montelli, Terezinha C. B; Peraçoli, Maria Terezinha S; Gabarra, Roberto C; Soares, Angela M. V. C; Kurokawa, Cilmery Suemi.
Título: Familial cancer: depressed NK-cell cytotoxicity in healthy and cancer affected members
Fonte: Arq. neuropsiquiatr;59(1):6-10, Mar. 2001. ilus, tab.
Idioma: en.
Resumo: Depressed natural killer (NK) cell activity has been showed in family members of patients with different types of cancer. The present work aimed to evaluate T cell subsets and NK cell cytotoxic activity in 15 members of a family with high incidence of tumors, such as glioblastoma, gastric, pancreas and colon rectal carcinoma, chronic myelocitic leukemia, melanoma and osteoblastoma. As controls, 19 healthy subjects with the age range equivalent were studied. The enumeration of CD3+ lymphocytes and their CD4+ and CD8+ subsets were defined by monoclonal antibodies and NK cell cytotoxicity towards K562 target cells were evaluated by single cell-assay. The results showed in family members low percentage of total T cells (CD3+), and their CD4+ subset and impairment of CD4/CD8 ratio in relation to control group. All family members presented percentage of NK-target cell conjugate formation bellow the minimum value observed in control group. Thirteen people were examined and followed up during five years, in order to assure that there was no undiagnosed or unsuspected disease at the moment of evaluation. One of them developed osteoblastoma and other malignant melanoma. Two cancer patients, with glioblastoma and chronic myelocytic leukemia were studied during illness. All the corresponding values were comparable. The persistence of low percentage of conjugate formation may be related to a defect on adhesion molecules expression in the surface of NK cells that was probably responsible for the low activity of these cells presented by the family group. Thus, the inheritance mechanism of low adherence of NK cells should have a prognostic value in determining the risk of developing tumors
Descritores: Citotoxicidade Imunológica
Células Matadoras Naturais/imunologia
Neoplasias/imunologia
Linfócitos T Citotóxicos/imunologia
-Anticorpos Monoclonais/imunologia
Complexo CD3/imunologia
Antígenos CD4/imunologia
Estudos de Casos e Controles
Moléculas de Adesão Celular/genética
Testes Imunológicos de Citotoxicidade
Glioblastoma/genética
Glioblastoma/imunologia
Subpopulações de Linfócitos
Neoplasias/genética
Linhagem
Estatísticas não Paramétricas
Limites: Humanos
Masculino
Feminino
Criança
Adolescente
Adulto
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Id: lil-92406
Autor: Leal, L. M. C; Dosreis, G. A.
Título: Independent and cooperative bheravior of THY1 and CD3:TcR T cell signalling pathways
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;23(9):835-9, 1990. ilus.
Idioma: en.
Resumo: Normal resting spleen T lymphocytes form mice were stimulated in vitro by monoclonal antibodies (mAbs) against either Thy1 or CD3:TcR surface protein molecules. Although both mAbs were mitogenic, anti-Thy1 activation generated 5 times more IL2 secretiom than anti-CD3 activation under similar conditions. Priduction of IL-like activity was comparable for both Thy1 and CD3-mediated activation. In addition, non-mitogenic doses of anti-CD3 and anti-Thy1 (0.16microng/ml and 0.0125% ascites, repectively) mAbs induced T cell activation when provided together. These reults indicate that Thy1 signalling cooperates with the CD3:TcR pathway to activate T cells. However, the pathway is also regulated independently since IL2 production is larger when stimulated by anti-Thy1 than anti-CD3mAbs
Descritores: Anticorpos Monoclonais/fisiologia
Complexo CD3/fisiologia
Técnicas In Vitro
Ativação Linfocitária/efeitos dos fármacos
Linfócitos T
-Interleucina-2/biossíntese
Interleucina-3/biossíntese
Camundongos Endogâmicos BALB C
Limites: Animais
Camundongos
Responsável: BR26.1 - Biblioteca Central



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