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Borges, Maria de Fátima
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Id: biblio-1041362
Autor: Borges, Maria de Fátima; Domené, Horacio Mario; Scaglia, Paula Alejandra; Lara, Beatriz Hallal Jorge; Palhares, Heloísa Marcelina da Cunha; Santos, Andréia Vasconcelos Aguiar; Gonçalves, Amanda Lacerda Ferreira; Oliveira, Marília Matos; Marqui, Alessandra Bernadete Trovó de.
Título: A recurrent mutation in tshb gene underlying central congenital hypothyroidism undetectable in neonatal screening / Uma mutação recorrente no gene tshb resultando em hipotireoidismo congênito central não detectável na triagem neonatal
Fonte: Rev. Paul. Pediatr. (Ed. Port., Online);37(4):520-524, Oct.-Dec. 2019. tab, graf.
Idioma: en.
Resumo: ABSTRACT Objective: To describe the case of a patient with central congenital hypothyroidism (CCH) due to a recurrent mutation in the TSHB gene, as well as to conduct a genetic study of his family. Case description: It is presented a case report of a 5-month-old boy with a delayed diagnosis of isolated CCH in whom the molecular analysis was performed 12 years later and detected a recurrent mutation (c.373delT) in TSHB gene. The parents and sister were carriers of the mutant allele. Comments: The c.373delT mutation has previously been reported in patients from Brazil, Germany, Belgium, United States, Switzerland, Argentina, France, Portugal, United Kingdom and Ireland. In summary, our case and other ones reported in the literature support the theory that this mutation may be a common cause of isolated TSH deficiency. Isolated TSH deficiency is not detected by routine TSH-based neonatal screening, representing a clinical challenge. Therefore, when possible, molecular genetic study is indicated. Identification of affected and carriers allows the diagnosis, treatment and adequate genetic counseling.

RESUMO Objetivo: Descrever o caso de um paciente com hipotireoidismo congênito central (HCC) por conta de uma mutação recorrente no gene TSHB, bem como realizar um estudo genético de sua família. Descrição do caso: Relato de caso de um menino de 5 meses de idade com diagnóstico tardio de HCC isolado, em quem a análise molecular foi realizada 12 anos depois e detectou uma mutação recorrente (c.373delT) no gene TSHB. Os pais e a irmã eram portadores do alelo mutante. Comentários: A mutação c.373delT já foi relatada em pacientes do Brasil, da Alemanha, da Bélgica, dos Estados Uinidos, da Suíça, da Argentina, da França, de Portugal, do Reino Unido e da Irlanda. Em resumo, nosso caso e outros relatados na literatura reforçam a teoria de que essa mutação pode ser uma causa comum de deficiência isolada de TSH. A deficiência isolada de TSH não é detectada na triagem neonatal com base na dosagem de TSH, representando um desafio clínico. Portanto, quando possível, o estudo genético molecular é indicado. A identificação dos afetados e dos portadores permite o diagnóstico, o tratamento e o aconselhamento genético adequado.
Descritores: Triagem Neonatal
Hipotireoidismo Congênito/diagnóstico
Tireotropina Subunidade beta/genética
Diagnóstico Tardio
Mutação
-Marcadores Genéticos
Hipotireoidismo Congênito/genética
Limites: Seres Humanos
Masculino
Feminino
Recém-Nascido
Lactente
Criança
Adulto
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: lil-792428
Autor: Picciani, Bruna Lavinas Sayed; Domingos, Tábata Alves; Teixeira-Souza, Thays; Santos, Vanessa de Carla Batista dos; Gonzaga, Heron Fernando de Sousa; Cardoso-Oliveira, Juliana; Gripp, Alexandre Carlos; Dias, Eliane Pedra; Carneiro, Sueli.
Título: Geographic tongue and psoriasis: clinical, histopathological, immunohistochemical and genetic correlation - a literature review
Fonte: An. bras. dermatol;91(4):410-421, July-Aug. 2016. tab, graf.
Idioma: en.
Resumo: Abstract: Geographic tongue is a chronic, inflammatory, and immune-mediated oral lesion of unknown etiology. It is characterized by serpiginous white areas around the atrophic mucosa, which alternation between activity, remission and reactivation at various locations gave the names benign migratory glossitis and wandering rash of the tongue. Psoriasis is a chronic inflammatory disease with frequent cutaneous involvement and an immunogenetic basis of great importance in clinical practice. The association between geographic tongue and psoriasis has been demonstrated in various studies, based on observation of its fundamental lesions, microscopic similarity between the two conditions and the presence of a common genetic marker, human leukocyte antigen (HLA) HLA-C*06. The difficulty however in accepting the diagnosis of geographic tongue as oral psoriasis is the fact that not all patients with geographic tongue present psoriasis. Some authors believe that the prevalence of geographic tongue would be much greater if psoriatic patients underwent thorough oral examination. This study aimed to develop a literature review performed between 1980 and 2014, in which consultation of theses, dissertations and selected scientific articles were conducted through search in Scielo and Bireme databases, from Medline and Lilacs sources, relating the common characteristics between geographic tongue and psoriasis. We observed that the frequency of oral lesions is relatively common, but to establish a correct diagnosis of oral psoriasis, immunohistochemical and genetic histopathological analyzes are necessary, thus highlighting the importance of oral examination in psoriatic patients and cutaneous examination in patients with geographic tongue.
Descritores: Psoríase/genética
Psoríase/patologia
Língua/patologia
Glossite Migratória Benigna/genética
Glossite Migratória Benigna/patologia
-Psoríase/complicações
Língua Fissurada/patologia
Biópsia
Imuno-Histoquímica
Marcadores Genéticos
Glossite Migratória Benigna/complicações
Glossite Migratória Benigna/terapia
Antígenos HLA/análise
Ilustração Médica
Limites: Seres Humanos
Masculino
Feminino
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-869086
Autor: Ruoti Cosp, Miguel.
Título: Análisis de los resultados de amniocentesis genética en un centro privado / Analysis of results of genetic amniocentesis in a private center
Fonte: Mem. Inst. Invest. Cienc. Salud (Impr.);14(2):75-83, ago. 2016. tab.
Idioma: es.
Resumo: La identificación de anomalías cromosómicas fetales es una de las principales tareas a las que debe enfrentarse cualquier obstetra involucrado en el diagnóstico de anomalías congénitas. Se analizaron características clínicas y citogenéticas en gestantes sometidas a amniocentesis. Estudio observacional, descriptivo y retrospectivo, incluyó casos consecutivos en un centro privado de julio de 2010 a enero de 2015. Las muestras fueron procesadas en CGC Genetic (Porto-Portugal). Para el análisis estadístico se utilizó PEPI 4.0X. Se realizaron 67 estudios, en el 98,5% se pudo obtener el cariotipo y de éstos resultaron 74,2% normales y 25,8% anormales: 35,4% Trisomía 21, 17,6% Trisomía 18, Trisomía 13 y Sx Turner respectivamente, entre las principales. Indicaciones: 6,0% edad materna, 14,9% edad materna + alteración ecográfica, 77,6% alteración ecográfica (45,2 malformaciones congénitas mayores, 20,1% translucencia nucal aumentada, 17,7% higroma quístico entre otras). La edad gestacional promedio de la punción fue 19 semanas, la menor a las 15 y la mayor a las 30. El resultado del cariotipo se recibió 12 días posteriores a la técnica en promedio, mínimo 8 días y máximo 29. No se presentaron complicaciones obstétricas. El seguimiento de los casos encontró concordancia entre el cariotipo y el fenotipo del recién nacido. Si bien es un número bajo de muestras, la amniocentesis es un método diagnóstico confiable y de bajo riesgo. El diagnóstico prenatal de cromosomopatías permitió el asesoramiento genético y el manejo obstétrico y pediátrico de los casos de manera adecuada. En los embarazos con cariotipo normal, este resultado alivió la preocupación de muchos padres.

Identification of fetal chromosomal abnormalities is one of the main issues which need tobe addressed by any obstetrician involved in diagnosis of congenital anomalies. Clinical andcytogenetic characteristics were analyzed in pregnant women subjected to amniocentesis.This was an observational descriptive retrospective study that included consecutive cases ina private center from July, 2010 to January, 2015. The samples were processed in CGCGenetic (Porto - Portugal). For the statistical analysis, PEPI 4.0X. was used. Sixty sevenstudies were conducted, in 98.5% karyotype was obtained , 74.2% was normal and 25.8%abnormal: 35.4% Trisomy 21, and 17.6% Trisomy 18, Trisomy 13 and Sx Turnerrespectively. Among the the main indications: 6.0% maternal age, 14.9% maternal ageplus ultrasound alteration, 77.6% ultrasound alteration (45.2% major congenital malformations, 20.1 increased nuchal translucency , 17.7% cystic hygroma among others).The mean gestational age of the puncture was 19 weeks, the lowest 15 and the highest 30. The result of the karyotype was received 12 days after the technique, the minimum 8 daysand the maximum 29. There were no obstetric complications. The follow-up of cases found concordance between the karyotype and the phenotype of the newborn. Although this was alow number of samples, the amniocentesis was reliable diagnostic method with low risk. Theprenatal diagnosis of chromosomopathies allowed the genetic counseling and appropiate obstetric and pediatric management cases. In pregnancies with normal karyotype, thisresult alleviated the concern of many parents.
Descritores: Amniocentese
Aneuploidia
Anormalidades Congênitas/diagnóstico
-Marcadores Genéticos
Limites: Seres Humanos
Feminino
Gravidez
Responsável: PY3.1 - Biblioteca


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Id: lil-781366
Autor: Bilgic, Özlem; Altınyazar, Hilmi Cevdet; Eryılmaz, Dilek; Tuğrul, Zehra Ayça.
Título: Are 2D: 4D finger-length ratios an indicator of androgenetic alopecia in males?
Fonte: An. bras. dermatol;91(2):156-159, Mar.-Apr. 2016. tab.
Idioma: en.
Resumo: Abstract BACKGROUND: Although the pathogenesis of androgenetic alopecia is not completely understood, the roles of genetic susceptibility and androgens are well-known. A lower ratio of the second digit (index finger = 2D) to the fourth digit (ring finger = 4D) length has been hypothesized to reflect prenatal androgen exposure and/or higher sensitivity to androgens. OBJECTIVES: To determine the relationship between the second to fourth digit length ratio and androgenetic alopecia. METHODS: Finger length measurements were made by a digital vernier calliper. Androgenetic alopecia severity was assessed using the Hamilton-Norwood scale. Subjects with an androgenetic alopecia score of grade III or more were included in the study. RESULTS: A total of 189 males with androgenetic alopecia and 171 healthy controls were enrolled in the study. The age range of participants was 19-65 years. The 2D:4D ratios in patients with androgenetic alopecia were significantly lower than those of healthy controls for the right hand; however, no significant difference was found for the left hand. Average 2D:4D ratios in androgenetic alopecia patients were also lower than in controls. No significant relationship was observed between androgenetic alopecia severity and 2D:4D ratios. CONCLUSION: Our data support the anatomical evidence of in utero androgen exposure and/or an individual’s sensitivity to androgens in patients with androgenetic alopecia. Furthermore, the right hand 2D:4D ratio might be an indicator of androgenetic alopecia development.
Descritores: Efeitos Tardios da Exposição Pré-Natal/diagnóstico
Alopecia/diagnóstico
Dedos/anatomia & histologia
-Tamanho do Órgão
Padrões de Referência
Valores de Referência
Índice de Gravidade de Doença
Gravidez
Marcadores Genéticos
Estudos de Casos e Controles
Antropometria/métodos
Valor Preditivo dos Testes
Reprodutibilidade dos Testes
Predisposição Genética para Doença
Alopecia/etiologia
Androgênios/análise
Androgênios/fisiologia
Limites: Seres Humanos
Masculino
Feminino
Adulto
Idoso
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-1049513
Autor: Rauschemberger, María Belén.
Título: DNI óseo: cuando los huesos hablan / Bone DNI: when the bones speak
Fonte: Actual. osteol;14(3):165-167, sept. - dic. 2018.
Idioma: es.
Descritores: DNA/genética
Osteologia
-Arqueologia
Esqueleto
Osso e Ossos/fisiologia
DNA/análise
Diagnóstico por Imagem
Marcadores Genéticos/genética
Limites: Seres Humanos
Masculino
Feminino
Tipo de Publ: Editorial
Responsável: AR2.1 - Biblioteca Central


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Id: biblio-1048783
Autor: Sanchez-Castro, Enrique Eduardo; La Torre-Ramírez, Renato; Padilla Rojas, Carlos Patricio; Lizaraso-Soto, Frank; Calderón Ticona, Jorge; Solís Villanueva, José; García-Quispes, Wilser Andrés; Paredes Anaya, Mónica Yolanda.
Título: Análisis de asociación entre polimorfismos (rs941798 y rs914458) del gen PTPN1 y diabetes tipo 2 en familias de Lima-Perú / Analysis of the association between PTPN1 gene polymorphisms (rs941798 and rs914458) and type 2 diabetes in families from Lima ­ Peru
Fonte: Horiz. méd. (Impresa);19(4):14-19, Dic. 2019. tab.
Idioma: es.
Resumo: Objetivo: Analizar la asociación de los polimorfismos de un solo nucleótido (SNP) rs941798 y rs914458 del gen PTPN1 con la diabetes tipo 2 en familias peruanas de Lima. Materiales y métodos: Veintitrés tríos familiares fueron captados en el Hospital Nacional Arzobispo Loayza. Se extrajeron muestras de sangre periférica para obtener el ADN y luego las frecuencias alélicas y genotípicas de los SNP. La genotipificación de los SNP se realizó mediante secuenciación. El análisis de asociación basado en familias entre los SNP y la diabetes tipo 2 se realizó con el programa FBAT. Resultados: Se observaron los 3 genotipos posibles para cada SNP, rs941798 (A>G) y rs914458 (G>C). Las pruebas de asociación basada en familias a nivel alélico mostraron al alelo A del SNP rs941798 asociado a la diabetes tipo 2 (p = 0.023) bajo uno de los modelos evaluados; no obstante, tras la corrección de Bonferroni para comparaciones múltiples, esta asociación se perdió. No se evidenció asociación entre los SNP y la enfermedad en ningún nivel (alélico, genotípico o haplotípico). Conclusiones: No se encontraron evidencias de asociación significativa entre los SNP rs941798 y rs914458 del gen PTPN1 con la DT2 en familias peruanas de Lima, en ninguno de los niveles estudiados (alélico, genotípico y haplotípico).

Objective: To analyze the association that PTPN1 gene single nucleotide polymorphisms (SNPs) rs941798 and rs914458 have with type 2 diabetes in Peruvian families from Lima. Materials and methods: Twenty-three (23) families consisting of three members each were recruited at the Hospital Nacional Arzobispo Loayza. Peripheral blood samples were collected to obtain the DNA, and then the allele and genotype frequencies of the SNPs. SNP genotyping was performed using the sequencing method. The family-based analysis of the association between SNPs and type 2 diabetes was conducted using the family-based association test (FBAT) program. Results: Three (3) possible genotypes were observed for each SNP, i.e. rs941798 (A>G) and rs914458 (G>C). In one of the assessed models, the family-based association tests showed at the allele level that allele A of SNP rs941798 is associated with type 2 diabetes (p = 0.023). However, after using the Bonferroni correction for multiple comparisons, this association was lost. No association was demonstrated between the SNPs and the disease at any level (allele, genotype or haplotype). Conclusions: No evidence of significant association was found between PTPN1 gene SNPs rs941798 and rs914458 and type 2 diabetes at the studied levels (allele, genotype or haplotype) in Peruvian families from Lima.
Descritores: Diabetes Mellitus Tipo 2
-Marcadores Genéticos
Polimorfismo de Nucleotídeo Único
Proteína Tirosina Fosfatase não Receptora Tipo 1
Limites: Seres Humanos
Tipo de Publ: Artigo Clássico
Responsável: PE383.9


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Rodrigues, Dália dos Prazeres
Leal, Nilma Cintra
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Id: lil-426797
Autor: Theophilo, Grace Nazareth Diogo; Rodrigues, Dália dos Prazeres; Leal, Nilma Cintra; Hofer, Ernesto.
Título: Distribution of virulence markers in clinical and environmental Vibrio cholerae non-O1/non-O139 strains isolated in Brazil from 1991 to 2000
Fonte: Rev. Inst. Med. Trop. Säo Paulo;48(2):65-70, Mar,-Apr. 2006. tab.
Idioma: en.
Projeto: National Council for Research Support.
Resumo: Cento e setenta e nove amostras de V. cholerae não O1/não O139, isoladas de casos clínicos (139) e de meio ambiente (40), no período de 1991 a 2000 no Brasil, foram caracterizadas antigenicamente pelo National Institute of Health (Japão) e investigadas quanto ao seu potencial genético de virulência, representado pelos genes ctxA, zot, ace e tcpA. As análises fenotípicas revelaram extraordinária diversidade antigênica, com a ocorrência de 54 diferentes sorogrupos, com prevalência para O26 (7,8%). A técnica de PCR, empregada na detecção dos genes localizados no elemento genético CTX (ctxA, zot, ace) e na Ilha de Patogenicidade de Vibrio-VPI (tcpA), possibilitou a identificação de 27 cepas contendo qualquer um desses genes. O gene ctxA (codificador da sub-unidade A de CT), só foi evidenciado no sorogrupo O26, sendo também o único capaz de se apresentar com o cassete de virulência de forma intacta. Com base nos resultados obtidos deste estudo preliminar, admite-se a hipótese da potencialidade destas cepas, evoluir para raças epidêmicas.
Descritores: Proteínas de Bactérias/genética
DNA Bacteriano/genética
Genes Bacterianos/genética
/genética
VIBRIO CHOLERAE OACHONDROPLASIA/genética
Vibrio cholerae não O1/genética
-Brasil
Marcadores Genéticos
Reação em Cadeia da Polimerase
Técnica de Amplificação ao Acaso de DNA Polimórfico
/patogenicidade
VIBRIO CHOLERAE OACHONDROPLASIA/patogenicidade
Vibrio cholerae não O1/patogenicidade
Virulência/genética
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-743765
Autor: Jaramillo, Priscilla C; Fuentes, Katterina; Cortés, Carla; Cisternas, Carlos; Salazar, Luis A.
Título: Análisis de la interacción entre el polimorfismo Rs671 del gen ALDH2 y consumo de alcohol en individuos chilenos / Interaction between Rs671 polymorphism of the ALDH2 gene and alcohol consumption in Chilean individuals
Fonte: Int. j. morphol;33(1):68-72, Mar. 2015. ilus.
Idioma: es.
Projeto: Universidad Santo Tomás.
Resumo: El alcoholismo es un importante problema de salud pública. En los últimos años ha causado interés el metabolismo del alcohol, puesto que ha sido considerado un posible determinante biológico en la conducta de consumo. Variados estudios se han orientado a la búsqueda y comprensión de la influencia de polimorfismos, en genes que codifican para los principales sistemas enzimáticos que intervienen en el metabolismo hepático. El polimorfismo rs671 del gen que codifica la enzima ALDH2 ha sido asociado a un menor consumo de alcohol debido a la acumulación de acetaldehído en sangre. Diversos estudios indican que este polimorfismo es frecuente en países asiáticos y se considera un factor protector en los individuos que lo portan. Se incluyeron 207 individuos adultos no relacionados, a los cuales se les aplicó un cuestionario sobre consumo de alcohol. El polimorfismo rs671 fue analizado por la reacción de la polimerasa en cadena (PCR) seguida de restricción enzimática. Además, se determinaron los biomarcadores clásicos indirectos de consumo de alcohol, mediante técnicas enzimáticas y hematológicas. La frecuencia del genotipo homocigoto mutado AA para el polimorfismo rs671 fue 3,0% en sujetos consumidores de alcohol y 2,8% en el grupo no consumidor. La distribución de genotipos y las frecuencias alélicas para esta variante fueron semejantes entre los sujetos estudiados (p>0,05). Estos hallazgos sugieren que la variante rs671 del gen ALDH2 no está asociada al oconsumo de alcohol en los individuos estudiados.

Alcoholism is an important public health problem. In recent years, alcohol metabolism caused interest, since it has been considered a possible biological determinant of alcohol consumption behavior. Several studies have focused on finding and understanding the influence of polymorphisms affecting genes that encode for enzymatic systems involved in the hepatic metabolism. The rs671 polymorphism of the gene encoding ALDH2 has been associated with lower alcohol consumption by leading to acetaldehyde accumulation in blood. This genetic variant is frequently found in Asian population and has been considered as protector factor of alcoholism in these individuals. In the present study, 207 unrelated-adult individuals were included. Alcohol consumption was recorded using a structured questionnaire. The rs671 polymorphism was analyzed using polymerase chain reaction followed by enzymatic digestion. Furthermore, classical biomarkers for alcohol consumption were assessed using enzymatic and hematological techniques. The frequency of homozygote genotype for the A allele (AA) was 3 and 2.8% in those subjects defined as alcohol drinkers and non-alcohol drinkers respectively. The genotypes distribution and allelic frequencies were similar among the studied subject (p>0.05). These data suggest that rs671 ALDH2 gene polymorphism is not associated to alcohol consumption in the studied population.
Descritores: Polimorfismo de Nucleotídeo Único
Alcoolismo/genética
Aldeído Desidrogenase/genética
-Polimorfismo Genético
Marcadores Genéticos
Chile
Reação em Cadeia da Polimerase
Inquéritos e Questionários
Alcoolismo/enzimologia
Alcoolismo/psicologia
Aldeído Desidrogenase/metabolismo
Limites: Seres Humanos
Masculino
Feminino
Adulto
Meia-Idade
Idoso
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1022247
Autor: Osorio, Claudia E; Udall, Joshua A; Salvo-Garrido, Haroldo; Maureira-Butler, Iván J.
Título: Development and characterization of InDel markers for Lupinus luteus L. (Fabaceae) and cross-species amplification in other Lupin species
Fonte: Electron. j. biotechnol;31:44-47, Jan. 2018. tab, graf.
Idioma: en.
Projeto: Comisión Nacional de Investigación Científica y Tecnológica Chile (CONICYT-Chile); . Centro de Genómica Nutricional Agroacuicola (CGNA), CONICYT-Regional.
Resumo: Background: Strong artificial selection and/or natural bottle necks may limit genetic variation in domesticated species. Lupinus luteus, an orphan temperate crop, has suffered diversity reductions during its bitter/sweet alkaloid domestication history, limiting breeding efforts and making molecular marker development a difficult task. The main goal of this research was to generate new polymorphic insertion­deletion (InDel) markers to aid yellow lupin genetics and breeding. By combining genomic reduction libraries and next generation sequencing, several polymorphic InDel markers were developed for L. luteus L. Results: A total of 118 InDel in silico polymorphic markers were identified. Eighteen InDel primer sets were evaluated in a diverse L. luteus core collection, where amplified between 2­3 alleles per locus. Observed heterozygosity (HO; 0.0648 to 0.5564) and polymorphic information content (PIC; 0.06 to 0.48) estimations revealed a moderate level of genetic variation across L. luteus accessions. In addition, ten and nine InDel loci amplified successfully Lupinus hispanicus Boiss & Reut, and Lupinus mutabilis Sweet, respectively, two L. luteus close relatives. PCA analysis identified two L. luteus clusters, most likely explained by the domestication species history. Conclusion: The development of InDel markers will facilitate the study of genetic diversity across L. luteus populations, as well as among closely related species.
Descritores: Variação Genética
Marcadores Genéticos
Lupinus/genética
Mutação INDEL
Sequenciamento de Nucleotídeos em Larga Escala
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1021043
Autor: Mei, Zhiqiang; Zhang, Xianqin; Khan, Md. Asaduzzaman; Imani, Saber; Liu, Xiaoyan; Zou, Hui; Wei, Chunli; Fu, Junjiang.
Título: Genetic analysis of Penthorum chinense Pursh by improved RAPD and ISSR in China
Fonte: Electron. j. biotechnol;30:6-11, nov. 2017. ilus, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Research Foundation of the Science and Technology Department of Sichuan Province Joint Program of the Science & Technology Office; . of Luzhou and the Science & Technology Department of Sichuan.
Resumo: Background: Penthorum chinense Pursh (P. chinense) is a well-known traditional Chinese medicine (TCM) plant, which has long been used for the prevention and treatment of hepatic diseases. This study aimed to genetically characterize the varieties of P. chinense from different geographic localities of China by random amplification of polymorphic DNA (RAPD)-PCR technique and verified with inter-simple sequence repeat (ISSR) markers. Results: The P. chinense samples were collected from nine different geographic localities. Previously improved RAPD and ISSR markers were utilized for genetic analysis using DNA amplification. The genetic relationship dendrogram was obtained by conducting cluster analysis to the similarity coefficient of improved RAPD and ISSR markers. Improved RAPD yielded 185 scorable amplified products, of which 68.6% of the bands were polymorphic, with an average amplification of 9.25 bands per primer. The ISSR markers revealed 156 alleles with 7.8 bands per primers, where 59.7% bands were polymorphic. Furthermore, the similarity coefficient ranges of RAPD and ISSR markers were 0.71­0.91 and 0.66­0.89, respectively. Conclusions: This study indicated that improved RAPD and ISSR methods are useful tools for evaluating the genetic diversity and characterizing P. chinense. Our findings can provide the theoretical basis for cultivar identification, standardization, and molecular-assisted breeding of P. chinense for medicinal use.
Descritores: Plantas Medicinais/genética
Magnoliopsida/genética
-Polimorfismo Genético
Variação Genética
Marcadores Genéticos
China
DNA de Plantas/genética
Técnica de Amplificação ao Acaso de DNA Polimórfico
Repetições de Microssatélites
Medicina Tradicional Chinesa
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