Base de dados : LILACS
Pesquisa : D25.479.517.500 [Categoria DeCS]
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Texto completo SciELO Brasil
Lima, Eliana Martins
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Id: lil-656663
Autor: Torres, Ieda Maria Sapateiro; Bento, Etiene Barbosa; Almeida, Larissa da Cunha; Sá, Luisa Zaiden Carvalho Martins de; Lima, Eliana Martins.
Título: Preparation, characterization and in vitro antimicrobial activity of liposomal ceftazidime and cefepime against Pseudomonas aeruginosa strains
Fonte: Braz. j. microbiol;43(3):984-992, July-Sept. 2012. graf, tab.
Idioma: en.
Resumo: Pseudomonas aeruginosa is an opportunistic microorganism with the ability to respond to a wide variety of environmental changes, exhibiting a high intrinsic resistance to a number of antimicrobial agents. This low susceptibility to antimicrobial substances is primarily due to the low permeability of its outer membrane, efflux mechanisms and the synthesis of enzymes that promote the degradation of these drugs. Cephalosporins, particularty ceftazidime and cefepime are effective against P. aeruginosa, however, its increasing resistance has limited the usage of these antibiotics. Encapsulating antimicrobial drugs into unilamellar liposomes is an approach that has been investigated in order to overcome microorganism resistance. In this study, antimicrobial activity of liposomal ceftazidime and cefepime against P. aeruginosa ATCC 27853 and P. aeruginosa SPM-1 was compared to that of the free drugs. Liposomal characterization included diameter, encapsulation efficiency and stability. Minimum Inhibitory Concentration (MIC) was determined for free and liposomal forms of both drugs. Minimum Bactericidal Concentration (MBC) was determined at concentrations 1, 2 and 4 times MIC. Average diameter of liposomes was 131.88 nm and encapsulation efficiency for cefepime and ceftazidime were 2.29% end 5.77%, respectively. Improved stability was obtained when liposome formulations were prepared with a 50% molar ratio for cholesterol in relation to the phospholipid. MIC for liposomal antibiotics for both drugs were 50% lower than that of the free drug, demonstrating that liposomal drug delivery systems may contribute to increase the antibacterial activity of these drugs.
Descritores: Antibacterianos/análise
Permeabilidade da Membrana Celular
Suscetibilidade a Doenças
Resistência Microbiana a Medicamentos
Técnicas In Vitro
Lipossomas Unilamelares/análise
Lipossomas Unilamelares/isolamento & purificação
Infecções por Pseudomonas
Pseudomonas aeruginosa/isolamento & purificação
-Alteração Ambiental
Métodos
Permeabilidade
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-607529
Autor: El-Nakeeb, Moustafa A; Abou-Shleib, Hamida M; Khalil, Amal M; Omar, Hoda G; El-Halfawy, Omar M.
Título: Membrane permeability alteration of some bacterial clinical isolates by selected antihistaminics
Fonte: Braz. j. microbiol;42(3):992-1000, July-Sept. 2011. ilus, tab.
Idioma: en.
Resumo: Several antihistaminics possess antibacterial activity against a broad spectrum of bacteria. However, the exact mechanism of such activity was unclear. Hence, the aim of this study is to investigate their mechanism of antibacterial activity especially their effect upon the permeability of the bacterial cytoplasmic membrane. The effects of azelastine, cetirizine, cyproheptadine and diphenhydramine were studied using Gram-positive and Gram-negative multiresistant clinical isolates. Leakage of 260 and 280 nm UV-absorbing materials was detected upon treatment with the tested antihistaminics; indicative of membrane alteration. Using an artificial membrane model, cholesterol-free negatively-charged unilamellar liposomes, confirmed the effect of antihistaminics upon the membrane permeability both by showing an apparent membrane damage as observed microscopically and by detection of leakage of preloaded dye from the liposomes colorimatrically. Moreover, examination of the ultrastructure of cells treated with azelastine and cetirizine under the transmission electron microscope substantiated the detected abnormalities in the cell wall and membrane. Furthermore, the effect of pretreating certain isolates for both short and long periods with selected antihistaminics was followed by the viable count technique. Increased vulnerability towards further exposure to azelastine was observed in cells pretreated with azelastine for 2 days and those pretreated with azelastine or cetrizine for 30 days.
Descritores: Membrana Celular
Permeabilidade da Membrana Celular
Parede Celular
Citoplasma
Resistência Microbiana a Medicamentos
Antagonistas dos Receptores Histamínicos H1
Lipossomas Unilamelares/análise
Lipossomas Unilamelares/farmacologia
-Métodos
Métodos
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica



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