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Id: lil-326427
Autor: Santos, B. C; Starobinas, N; Barbuto, J. A. M; Russo, M; Schor, N.
Título: Absence of peripheral blood mononuclear cells priming in hemodialysis patients
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;36(2):219-225, Feb. 2003. graf.
Idioma: en.
Resumo: As a consequence of the proinflammatory environment occurring in dialytic patients, cytokine overproduction has been implicated in hemodialysis co-morbidity. However, there are discrepancies among the various studies that have analyzed TNF-alpha synthesis and the presence of peripheral blood mononuclear cell (PBMC) priming in this clinical setting. We measured bioactive cytokine by the L929 cell bioassay, and evaluated PBMC TNF-alpha production by 32 hemodialysis patients (HP) and 51 controls. No difference in TNF-alpha secretion was observed between controls and HP (859 ± 141 vs 697 ± 130 U/10(6) cells). Lipopolysaccharide (5 æg/ml) did not induce any further TNF-alpha release, showing no PBMC priming. Paraformaldehyde-fixed HP PBMC were not cytotoxic to L929 cells, suggesting the absence of membrane-anchored TNF-alpha. Cycloheximide inhibited PBMC cytotoxicity in HP and controls, indicating lack of a PBMC TNF-alpha pool, and dependence on de novo cytokine synthesis. Actinomycin D reduced TNF-alpha production in HP, but had no effect on controls. Therefore, our data imply that TNF-alpha production is an intrinsic activity of normal PBMC and is not altered in HP. Moreover, TNF-alpha is a product of de novo synthesis by PBMC and is not constitutively expressed on HP cell membranes. The effect of actinomycin D suggests a putative tighter control of TNF-alpha mRNA turnover in HP. This increased dependence on TNF-alpha RNA transcription in HP may reflect an adaptive response to hemodialysis stimuli
Descritores: Leucócitos Mononucleares
Citocinas
Diálise Renal
Fator de Necrose Tumoral alfa
-Leucócitos Mononucleares
Inibidores da Síntese de Proteínas
Estudos de Casos e Controles
Cicloeximida
Dactinomicina
Limites: Humanos
Adulto
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  2 / 23 LILACS  
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Id: lil-723171
Autor: Cai, Cheng; Qiu, Gang; Gong, Xiaohui; Chen, Yihuan; Zhao, Huanhu.
Título: Effects of erythromycin on γ-glutamyl cysteine synthetase and interleukin-1β in hyperoxia-exposed lung tissue of premature newborn rats / Efeitos da eritromicina sobre a γ-glutamil-cisteína-sintetase e a interleucina-1β no tecido pulmonar exposto à hiperóxia de ratos recém-nascidos prematuros
Fonte: J. pediatr. (Rio J.);90(5):493-499, Sep-Oct/2014. graf.
Idioma: en.
Projeto: Shanghai Science and Technology Committee.
Resumo: Objective: To explore the effect of erythromycin on hyperoxia-induced lung injury. Methods: One-day-old preterm offspring Sprague-Dawley (SD) rats were randomly divided into four groups: group 1, air + sodium chloride; group 2, air + erythromycin;group 3, hyperoxia + sodium chloride; and group 4, hyperoxia + erythromycin. At one, seven, and 14 days of exposure, glutathione (GSH) and interleukin-1 beta (IL-1 beta) were detected by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA), and bicinchoninic acid (BCA) was used to detect GSH protein. γ-glutamine-cysteine synthetase (γ-GCS) mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results: Compared with group 1, expressions of GSH and γ-GCS mRNA in group 3 were significantly increased at one and seven days of exposure (p < 0.05), but expression of γ-GCS mRNA was significantly reduced at 14 days; expression of IL-1 beta in group 3 was significantly increased at seven days of exposure (p < 0.05), and was significantly reduced at 14 days. Compared with group 3, expressions of GSH and γ-GCS mRNA in group 4 were significantly increased at one, seven, and 14 days of exposure (p < 0.05), but expressions of GSH showed a downward trend at 14 days; expression of IL-1 beta in group 4 was significantly reduced at one and seven days of exposure (p < 0.05). Conclusions: Changes in oxidant-mediated IL-1 beta and GSH are involved in the development of hyperoxia-induced lung injury. Erythromycin may up-regulate the activity of γ-GCS, increasing the expression of GSH, inhibiting the levels of oxidant-mediated IL-1 beta and alleviating hyperoxia-induced lung injury via an antioxidant effect. .

Objetivo: Explorar o efeito da eritromicina sobre lesões pulmonares induzidas por hiperóxia. Métodos: Uma prole de ratos Sprague-Dawley (SD) prematuros com um dia de vida foi dividida aleatoriamente em quatro grupos: grupo 1 ar + cloreto de sódio, grupo 2 ar + eritromicina, grupo 3 hiperóxia + cloreto de sódio e grupo 4 hiperóxia + eritromicina. Com um, sete e 14 dias de exposição, foram detectadas Glutationa (GSH) e Interleucina-1 beta (IL-1 beta) pelo ensaio imunossorvente ligado à enzima (ELISA), e o ácido bicinconinico (BCA) foi utilizado para detectar a proteína GSH. O mRNA da γ-glutamil-cisteina-sintetase (γ-GCS) foi detectado por reação em cadeia da polimerase via transcriptase reversa (RT-PCR). Resultados: Comparadas ao grupo 1, as expressões do mRNA da GSH e da γ-GCS no grupo 3 aumentaram significativamente com um e sete dias de exposição (p < 0,05), porém a expressão de mRNA da γ-GCS diminuiu significativamente aos 14 dias; a expressão de IL-1 beta no grupo 3 aumentou significativamente aos 7 dias de exposição (p < 0,05) e diminuiu significativamente aos 14 dias. Comparadas ao grupo 3, as expressões do mRNA da GSH e da γ-GCS no grupo 4 aumentaram significativamente com um, sete e 14 dias de exposição (p < 0,05), porém as expressões de GSH mostraram uma tendência de queda aos 14 dias; a expressão de IL-1 beta no grupo 4 foi reduzida significativamente com um e sete dias de exposição (p < 0,05). Conclusões: As variações de IL-1 beta e GSH mediadas por oxidantes estão envolvidas no desenvolvimento de lesão pulmonar induzida por hiperóxia. A eritromicina poderá regular positivamente a atividade da γ-GCS, aumentando a expressão de GSH, inibindo os níveis de interleucina-1beta mediada por ...
Descritores: Eritromicina/farmacologia
Glutamato-Cisteína Ligase/efeitos dos fármacos
Hiperóxia/metabolismo
Interleucina-1beta/efeitos dos fármacos
Pulmão/efeitos dos fármacos
Inibidores da Síntese de Proteínas/farmacologia
-Animais Recém-Nascidos
Modelos Animais de Doenças
Ensaio de Imunoadsorção Enzimática
Glutationa/metabolismo
Interleucina-1beta/metabolismo
Lesão Pulmonar/metabolismo
Oxigênio/metabolismo
Oxigênio/farmacologia
Inibidores da Síntese de Proteínas/metabolismo
Distribuição Aleatória
Ratos Sprague-Dawley
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limites: Animais
Feminino
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  3 / 23 LILACS  
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Id: lil-652677
Autor: Vulgarin Martínez, Luis.
Título: Cloranfenicol: un aliado olvidado, revisión bibliográfica / Chloramphenicol: a forgotten ally, bibliographic review
Fonte: Medicina (Guayaquil);11(3):237-243, sept. 2006.
Idioma: es.
Resumo: El cloranfenicol es un antimicrobiano cuyo mecanismo de acción radica en la inhibición de la síntesis proteica bacteriana y posee un amplio espectro de acción. La dosificación en adultos y niños está dada no sólo por peso en kilos sino por la edad cronológica, en especial en estos últimos. Su uso actualmente está limitado a la salmonellosis tífica; pero tiene otras aplicaciones como en la meningitis por meningococo, en especial en los pacientes alérgicos a los betalactámicos. Sus reacciones adversas como la aplasia medular y el síndrome del niño gris, se presentan en 1 por 30.000 y l por 70.000 pacientes respectivamente; siendo causadas por idiosincrasia y por el uso de dosis superiores a los rangos terapéuticos.

Chloramphenicol is a bacterostatic agent it inhibits bacterial protein synthesis and it has a wide spectrum activity. The dosage in adults and children depends not only on weight but on chronological age. Its use is limited to salmonella typi but can also be used for Meningococcal Meningitis in patients who are allergic to beta lactamics. The adverse reaction are bone marrow depression and the Gray Baby Syndrome present in 1/ 30.000 and 1 / 70.000 respectively which have idiosyncratic cause and also from use of high doses.
Descritores: Antibacterianos
Infecções Bacterianas
Cloranfenicol
Inibidores da Síntese de Proteínas
-Infecções por Bactérias Gram-Negativas
Infecções por Bactérias Gram-Positivas
Infecções por Spirochaetales
Limites: Masculino
Adulto
Feminino
Criança
Tipo de Publ: Guia de Prática Clínica
Responsável: EC13.1 - Biblioteca


  4 / 23 LILACS  
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Id: lil-541111
Autor: Novak, I. T; Cabral, H. R.
Título: Rosette formation by macrophages with adhered T lymphocytes is precluded by inhibitors of antigen processing and presentation
Fonte: Biocell;32(2):169-174, Aug. 2008. ilus, graf.
Idioma: en.
Resumo: We had previously found in autologous human leukocyte cultures, in which dead neutrophils phagocytosis by macrophages occur, macrophages and T CD4 lymphocytes perform a selective cell-cell interaction showing many figures of either one, two or several T- lymphocytes adhering to a central macrophage were seen. Considering that antigen presentation would be necessary for the formation of these immune synapses, we attempted to block rosette formation (i.e., the formation of macrophage associations with at least three lymphocytes) by interfering with both antigen processing and presentation. Culture samples of autologous leukocytes from 7 healthy donors were subjected to either brefeldin A, chloroquine or to an anti-HLA DR antibody. Rosette formation was significantly inhibited in the treated samples (either with brefeldin A, chloroquine or the anti- HLA DR; ANOVA, p<0.001, as compared with the untreated controls). It is concluded that interference with antigen processing and presentation precludes the formation of these cell-cell interactions.
Descritores: Adesão Celular/fisiologia
Antirreumáticos/farmacologia
Antígenos HLA-DR/imunologia
Brefeldina A/farmacologia
Cloroquina/farmacologia
Apresentação do Antígeno/imunologia
-Células Cultivadas
Inibidores da Síntese de Proteínas/farmacologia
Linfócitos T/citologia
Linfócitos T
Linfócitos T/imunologia
Macrófagos/citologia
Macrófagos
Macrófagos/imunologia
Limites: Humanos
Masculino
Adolescente
Adulto
Feminino
Pessoa de Meia-Idade
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo


  5 / 23 LILACS  
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Id: lil-498915
Autor: Oliveira-Martins, C. R; Grisolia, C. K.
Título: Determination of micronucleus frequency by acridine orange fluorescent staining in peripheral blood reticulocytes of mice treated topically with different lubricant oils and cyclophosphamide
Fonte: Genet. mol. res. (Online);6(3):566-574, 2007. ilus, tab, graf.
Idioma: en.
Resumo: To ascertain whether used and re-refined lubricant oil absorbed through the skin can produce a genotoxic effect or cytotoxicity in mouse bone marrow cells, we examined the induction of micronucleated erythrocytes of peripheral blood after cutaneous application. Both re-refined and used lubricant oils showed a weak but significant induction of micronucleated polychromatic erythrocytes compared with control, while virgin oil did not show micronucleus induction. Cyclophosphamide (CP) was used not only as positive control but also to compare the sensitivity between intraperitoneal and dermal routes of administration of the test compounds in mice. The efficacy of intraperitoneal injection of CP is well known. On the other hand, dermal exposure is not so common and when CP was diluted in glycerin statistically significant values (P = 0.0036) of micronuclei were also found. Topically applied lubricant oils (virgin, re-refined and used) have the capacity to interfere with mouse bone marrow hematopoiesis evidenced by a statistically significant decrease in the proportion of polychromatic erythrocytes in the peripheral blood. Physical and chemical analysis revealed that used oil is more viscous than other lubricants, suggesting the presence of insoluble compounds, oxidized products and water as well as aromatic hydrocarbons. Used oil differs from other lubricant oils in metal and polyaromatic hydrocarbon content. Re-refined oil revealed a neutral value typical of pure mineral oil. This assay is an important tool to evaluate environmental pollutants that cause genotoxicity and/or cytotoxicity through skin exposure.
Descritores: Ciclofosfamida/farmacologia
Inibidores da Síntese de Proteínas/farmacologia
Laranja de Acridina/farmacologia
Pele
Reticulócitos
-Corantes Fluorescentes/farmacologia
Microscopia de Fluorescência/métodos
Óleos
Pele/metabolismo
Reticulócitos/metabolismo
Coloração e Rotulagem
Testes para Micronúcleos/métodos
Limites: Animais
Masculino
Feminino
Ratos
Responsável: BR26.1 - Biblioteca Central


  6 / 23 LILACS  
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Id: lil-455755
Autor: Lana, R. P; Leopoldino, W. M; Oliveira, J. S; Veloso, R. G; Nunes, P. M. M; Queiroz, A. C.
Título: Parâmetros da degradação protéica ruminal de diferentes alimentos e rações estimados por técnica in vitro / Parameters of ruminal protein degradation of different feeds and diets estimated by an in vitro method
Fonte: Arq. bras. med. vet. zootec;59(2):414-422, abr. 2007. graf, tab.
Idioma: pt.
Resumo: Foram realizados três experimentos para estudar os parâmetros de degradação protéica ruminal. No primeiro, foram incubadas, em líquido ruminal de bovinos, dietas isoprotéicas contendo capim-elefante, fubá de milho e farelo de soja, em cinco níveis de concentrado (0:100, 25:75, 50:50, 75:25 e 100:0), adicionado ou não de monensina (5µM). Houve efeito linear decrescente do nível de concentrado sobre a concentração de amônia e degradabilidade da proteína bruta (DPB), e efeito cúbico sobre a concentração de proteína solúvel, com máximo valor em dieta com 25 por cento de concentrado. A monensina diminuiu a DPB e a concentração de proteína solúvel, sem afetar a produção de amônia. No segundo experimento, foram incubados cinco diferentes volumosos (silagens de milho - Zea mays L. e de capim-elefante - Pennisetum purpureum, silagem pré-secada de braquiária -Brachiaria decumbens, feno de Tifton 85 -Cynodon sp. amonizado e feno de Tifton 85). A silagem pré-secada de capim-braquiária e o feno de Tifton 85 amonizado apresentaram as maiores concentrações de amônia (8,7 e 5,3mg/dl) e proteína solúvel (5,4 e 7,0mg/dl), devido aos seus maiores teores de PB, seguidos da silagem de capim-elefante e feno de Tifton 85. A DPB variou de 29,6 a 80,6 por cento, para a silagem pré-secada de braquiária e para o feno de Tifton 85, e a degradabilidade potencial da matéria seca de 40,1 a 64,3 por cento, para a silagem de capim-elefante e silagem pré-secada de braquiária, respectivamente. A degradabilidade efetiva da proteína bruta apresentou baixos valores devido à baixa taxa de degradação da fração insolúvel. No terceiro experimento, foram incubados diferentes tipos de camas de frango (casca de café, capim-elefante seco picado, sabugo de milho ou cepilho), contendo ou não monensina (5µM). Não houve diferença nas concentrações de amônia entre as diferentes camas de frango, na ausência de monensina. Entretanto, com monensina, a cama de capim-elefante apresentou o...

Three experiments were carried out in order to study the parameters of ruminal protein degradation. In the first, isoproteic diets, constituted of elephant grass, ground corn and soybean meal, at five concentrate levels (0:100, 25:75, 50:50, 75:25 e 100:0), with or without monensin (5µM), were incubated in ruminal fluid of bovines. There was a decreasing linear effect of the concentrate level on ammonia concentration and degradability of crude protein, and cubic effect in soluble protein concentration, with the largest value in the diet with 25 percent concentrate. Monensin decreased degradability of crude protein and soluble protein concentration with no effect on ammonia production. In the second, five different roughages (corn and elephant grass silage - Pennisetum purpureum, Brachiaria haylage - Brachiaria decumbens, ammoniated Tifton 85 hay - Cynodon sp. e Tifton 85 hay). The were incubated Brachiaria haylage and the ammoniated Tifton 85 hay showed the greatest concentrations of ammonia (8.7 and 5.3mg/dl) and soluble protein (5.4 and 7.0mg/dl), due to their higher crude protein content, followed by elephant grass silage and Tifton 85 hay. The degradability of crude protein ranged from 29.6 to 80.6 percent for Brachiaria haylage and Tifton 85 hay, and the degradability of dry matter ranged from 40.1 to 64.3 percent for elephant grass silage and Brachiaria haylage, respectively. The effective degradability of crude protein showed low values due to low degradation rate of the insoluble fraction. In the third, four different poultry litter (hulls coffee, shopped dry elephant grass, corn cobs and wood shavings) were incubated, with or without monensin (5µM). No difference in ammonia concentration among the poultry litter samples, was observed in the absence of monensin. However, when monensin was present, the grass poultry litter showed the lowest ammonia level and wood poultry litter the highest. The poultry litter influenced the soluble...
Descritores: Bovinos
Inibidores da Síntese de Proteínas/metabolismo
Proteínas/metabolismo
Rúmen/fisiologia
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


  7 / 23 LILACS  
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Id: lil-392270
Autor: Urbina, Julio.
Título: Nuevos avances en el tratamiento específico de la enfermedad de chagas: ensayos experimentales con inhibidores de la síntesis de ergosterol / New advances in the treatment specify of the chagas disease: experimental rehearsals with inhibitors of the ergosterol synthesis
Fonte: Av. cardiol;21(3):86-87, sept. 2001.
Idioma: es.
Descritores: Doença de Chagas
Ergosterol
Inibidores da Síntese de Proteínas/uso terapêutico
-Cardiologia
Venezuela
Tipo de Publ: Revisão
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


  8 / 23 LILACS  
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Id: lil-319780
Autor: Rehen, S. K; Linden, R.
Título: Apoptosis in the developing retina: paradoxical effects of protein synthesis inhibition
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;27(7):1647-1651, Jul. 1994.
Idioma: en.
Resumo: Cell death by apoptosis is usually characterized as an active process that requires protein and RNA synthesis. The requirement of protein synthesis for the degeneration of ganglion cells and other cell types was studied in neural retinae explanted from the eyes of newborn rats. Ganglion cells were detected by the presence of retrogradely transported horseradish peroxidase injected into the superior colliculus. Apoptotic cells were recognized by their condensed and deeply stained chromatin. The data show that the death of ganglion cells, whose axons are damaged when preparing the explants, is blocked or delayed by protein synthesis inhibitors. In contrast, the blockade of protein synthesis produced cell death with apoptotic morphology in the neuroblastic layer of the same retinae. The results suggest the operation in the developing retina of both a program of apoptosis dependent on the synthesis of killer proteins, and a latent mechanism of apoptosis that is normally blocked by repressor proteins.
Descritores: Apoptose
Inibidores da Síntese de Proteínas/farmacologia
Retina
-Animais Recém-Nascidos
Morte Celular
Cicloexilaminas
Peroxidase do Rábano Silvestre
Degeneração Neural
Retina
Células Ganglionares da Retina
Limites: Animais
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  9 / 23 LILACS  
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Id: lil-307959
Autor: Ferreras, Ana; Triana, Ledia; Sánchez, Erlinda; Herrera, Flor.
Título: Effect of Antimalarial Drugs on Plasmodia Cell-Free Protein Synthesis
Fonte: Mem. Inst. Oswaldo Cruz;97(3):377-380, Apr. 2002. ilus, graf.
Idioma: en.
Resumo: A cell-free system from Plasmodium falciparum able to translate endogenous mRNA was used to determine the effect of artemisinin, chloroquine and primaquine on the protein synthesis mechanism of the parasite. The antimalarial drugs did not inhibit the incorporation of [³H] methionine into parasite proteins even at concentrations higher than the ones found to strongly inhibit the parasite growth. Results clearly indicate that these compounds do not have a direct effect on protein synthesis activity of P. falciparum coded by endogenous mRNA
Descritores: Antimaláricos
Plasmodium falciparum
Inibidores da Síntese de Proteínas
Proteínas de Protozoários
-Cloroquina
Eletroforese em Gel de Poliacrilamida
Plasmodium falciparum
Primaquina
Biossíntese de Proteínas
RNA Mensageiro
Sesquiterpenos
Limites: Animais
Responsável: BR1.1 - BIREME


  10 / 23 LILACS  
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Id: lil-302650
Autor: González Frugone, Ximena.
Título: Quinupristina, dalfopristina / Quinupristin, dalfopristin
Fonte: Bol. inf. medicam. (Santiago de Chile);18(2/3):11-14, dic. 2001. ilus.
Idioma: es.
Descritores: Inibidores da Síntese de Proteínas/farmacologia
-Bombas de Infusão
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central



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