Base de dados : LILACS
Pesquisa : D27.505.519.593.624 [Categoria DeCS]
Referências encontradas : 35 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 4 ir para página            

  1 / 35 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Id: biblio-836849
Autor: Sant'Ana, Adriana Campos Passanezi; Passanezi, Euloir; Rezende, Maria Lúcia Rubo de; Greghi, Sebastião Luiz Aguiar.
Título: Papel dos fatores de crescimento na regeneração periodontal: estado atual da arte / The role of growth factors in periodontal regeneration: current state-of-the-art
Fonte: Perionews;1(2):133-140, abr.-jun. 2007. ilus, tab.
Idioma: pt.
Resumo: Fatores de crescimento polipeptídicos são proteínas de baixo peso molecular sintetizadas por várias células e tecidos que regulam os eventos básicos que levam à regeneração periodontal: proliferação, quimiotaxia, diferenciação e síntese de matriz protéica. Embora diversos métodos terapêuticos tenham sido propostos para uso clínico visando a regeneração dos tecidos periodontais, a previsibilidade de sucesso ainda é limitada, com casos bem-sucedidos e outros mostrando resultados aquém do esperado. Recentemente, após a identificação da existência de células tronco no ligamento periodontal e a compreensão que a diferenciação destas células em outras mais diferenciadas (fibroblastos, osteoblastos e cementoblastos) é mediada por moléculas de sinalização (fatores de crescimento), alguns estudos foram publicados propondo a utilização clínica dos fatores de crescimento, isolados ou combinados, em diferentes tipos de veículos com o objetivo de favorecer a regeneração periodontal. Neste artigo, as características gerais e os estudos in vitro e in vivo evidenciando o papel dos principais fatores de crescimento utilizados na regeneração periodontal serão revistos com o objetivo de avaliar o papel dos fatores de crescimento na regeneração periodontal.

Polipeptidic growth factors are low density proteins secreted by different cells and tissues that regulate the basic events leading to periodontal regeneration: proliferation, chemotaxis, differentiation and protein synthesis. Although many methods for achieving regeneration of lost periodontal tissues have been suggested, there is poor predictability, with some well-succeeded cases and others showing unexpected results. Recently, after the identification of mesenchymal stem cells in the periodontal ligament and the understanding that the differentiation of these cells onto more differentiated ones (osteoblasts, cementoblasts, fibroblasts) is mediated by signaling molecules (growth factors), some studies have proposed the clinical use of growth factors isolated or in combination to enhance periodontal regeneration outcomes. In this paper, the characteristics and in vitro and the in vivo studies evaluating the role of growth factors in periodontal regeneration will be reviewed to evaluate the role of growth factors in periodontal regeneration.
Descritores: Quimiotaxia
Regeneração Tecidual Guiada Periodontal
Peptídeos e Proteínas de Sinalização Intercelular
Mitógenos
Periodontia
Limites: Seres Humanos
Tipo de Publ: Revisão
Responsável: BR510.1 - Biblioteca Central


  2 / 35 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Velloso, Elvira Deolinda Rodrigues Pereira
Texto completo
Id: lil-725679
Autor: Oliveira, Roberta Maria da Silva; Velloso, Elvira Deolinda Rodrigues Pereira.
Título: Detection of cytogenetic abnormalities in mature B-cell neoplasms: the value of cultures with different mitogens
Fonte: Rev. bras. hematol. hemoter;36(5):379-380, Sep-Oct/2014. tab.
Idioma: en.
Descritores: Linfócitos B
Aberrações Cromossômicas
Mitógenos
Neoplasias
Tipo de Publ: Carta
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


  3 / 35 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-716268
Autor: Wan, Y.; Tang, M.H.; Chen, X.C.; Chen, L.J.; Wei, Y.Q.; Wang, Y.S..
Título: Inhibitory effect of liposomal quercetin on acute hepatitis and hepatic fibrosis induced by concanavalin A
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(8):655-661, 08/2014. tab, graf.
Idioma: en.
Projeto: the Scientific Research Foundation for Young Teachers.
Resumo: Immune response plays an important role in the development of hepatic fibrosis. In the present study, we investigated the effects of quercetin on hepatitis and hepatic fibrosis induced by immunological mechanism. In the acute hepatitis model, quercetin (2.5 mg/kg) was injected iv into mice 30 min after concanavalin A (Con A) challenge. Mice were sacrificed 4 or 24 h after Con A injection, and aminotransferase tests and histopathological sections were performed. Treatment with quercetin significantly decreased the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Consistent with this observation, treatment with quercetin markedly attenuated the pathologic changes in the liver. A hepatic fibrosis model was also generated in mice by Con A challenge once a week for 6 consecutive weeks. Mice in the experimental group were treated with daily iv injections of quercetin (0.5 mg/kg). Histopathological analyses revealed that treatment with quercetin markedly decreased collagen deposition, pseudolobuli development, and hepatic stellate cells activation. We also examined the effects of quercetin on the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and transforming growth factor beta (TGF-β) pathways by immunohistochemistry and real-time reverse transcriptase-polymerase chain reaction (RT-PCR). NF-κB and TGF-β production was decreased after treatment with quercetin, indicating that the antifibrotic effect of quercetin is associated with its ability to modulate NF-κB and TGF-β production. These results suggest that quercetin may be an effective therapeutic strategy in the treatment of patients with liver damage and fibrosis.
Descritores: Antioxidantes/administração & dosagem
Hepatite/tratamento farmacológico
Cirrose Hepática/tratamento farmacológico
Quercetina/farmacologia
-Alanina Transaminase/sangue
Aspartato Aminotransferases/sangue
Concanavalina A
Colágeno/análise
Modelos Animais de Doenças
Células Estreladas do Fígado/efeitos dos fármacos
Células Estreladas do Fígado/metabolismo
Lipossomos
Cirrose Hepática/induzido quimicamente
Camundongos Endogâmicos BALB C
Mitógenos
NF-kappa B/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Fator de Crescimento Transformador beta/metabolismo
Limites: Animais
Feminino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 35 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-670899
Autor: Brazilian Journal of Medical and Biological Research; Cunha, E.V.; Costa, J.J.N.; Rossi, R.O.D.S.; Silva, A.W.B.; Passos, J.R.S.; Portela, A.M.L.R.; Pereira, D.C.S.T.; Donato, M.A.M.; Campello, C.C.; Saraiva, M.V.A.; Peixoto, C.A.; Silva, J.R.V.; Santos, R.P..
Título: Phytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicles
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;46(3):245-252, 15/mar. 2013. tab, graf.
Idioma: en.
Projeto: CNPq; . FUNCAP.
Resumo: The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles cultured in vitro. Secondary follicles (∼0.2 mm) were isolated from the cortex of caprine ovaries and cultured individually for 6 days in α-MEM+ supplemented with PHA (0, 1, 10, 50, 100, or 200 µg/mL). After 6 days of culture, follicle diameter and survival, antrum formation, ultrastructure and expression of mRNA for FSH receptors (FSH-R), proliferating cell nuclear antigen (PCNA), and neuronal nitric oxide synthase were determined. All treatments maintained follicular survival [α-MEM+ (94.59%); 1 µg/mL PHA (96.43%); 10 µg/mL PHA (84.85%); 50 µg/mL PHA (85.29%); 100 µg/mL PHA (88.57%), and 200 µg/mL PHA (87.50)], but the presence of 10 µg/mL PHA in the culture medium increased the antrum formation rate (21.21%) when compared with control (5.41%, P < 0.05) and ensured the maintenance of oocyte and granulosa cell ultrastructures after 6 days of culture. The expression of mRNA for FSH-R (2.7 ± 0.1) and PCNA (4.4 ± 0.2) was also significantly increased in follicles cultured with 10 µg/mL PHA in relation to those cultured in α-MEM+ (1.0 ± 0.1). In conclusion, supplementation of culture medium with 10 µg/mL PHA maintains the follicular viability and ultrastructure, and promotes the formation of antral cavity after 6 days of culture in vitro.
Descritores: Hormônio Foliculoestimulante/metabolismo
Mitógenos/farmacologia
Folículo Ovariano/efeitos dos fármacos
Fito-Hemaglutininas/farmacologia
-Hormônio Foliculoestimulante/genética
Cabras
Técnicas In Vitro
Folículo Ovariano/crescimento & desenvolvimento
Folículo Ovariano/ultraestrutura
Antígeno Nuclear de Célula em Proliferação/metabolismo
RNA Mensageiro/metabolismo
Limites: Animais
Feminino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  5 / 35 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-640207
Autor: Conti-Freitas, Luiz C.; Foss-Freitas, Maria C.; Mamede, Rui C. M.; Foss, Norma T..
Título: Interferon-gamma and interleukin-10 production by mononuclear cells from patients with advanced head and neck cancer
Fonte: Clinics;67(6):587-590, 2012. tab.
Idioma: en.
Resumo: OBJECTIVE: This study aims to evaluate the production of interferon-gamma and interleukin-10 by stimulated peripheral blood mononuclear cells isolated from patients with supraglottic laryngeal cancer before and after surgical treatment. METHODS: Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cultures of peripheral blood mononuclear cells isolated during the preoperative and late postoperative periods were stimulated with concanavalin A and Bacille Calmette-Guerin, and the supernatant concentrations of interferon-gamma and interleukin-10 were measured. RESULTS: For non-stimulated cultures, the interferon-gamma levels produced by the preoperative period and the late postoperative period cultures were lower than the levels produced by the control group cultures. The interferon-gamma levels after stimulation with concanavalin A were higher in the late postoperative period cultures than in the preoperative evaluation cultures. Stimulation with Bacille Calmette-Guerin led to the production of similar levels of interferon-gamma and interleukin-10 by all cultures; thus, stimulation increased the levels of interferon-gamma produced by both the preoperative and postoperative cultures relative to the levels produced by the corresponding unstimulated cultures. CONCLUSION: Patients with advanced supraglottic laryngeal cancer exhibit an in vitro deficiency in interferongamma secretion by mononuclear cells. Stimulated cells seem to recover this function during the postoperative period.
Descritores: Carcinoma/sangue
Interferon gama/biossíntese
/biossíntese
INTERLEUKIN-ABDUCENS NERVE/biossíntese
Neoplasias Laríngeas/sangue
Leucócitos Mononucleares/metabolismo
-Estudos de Casos e Controles
Carcinoma/patologia
Concanavalina A/farmacologia
Citocinas/sangue
Interferon gama/sangue
/sangue
INTERLEUKIN-ABDUCENS NERVE/sangue
Neoplasias Laríngeas/patologia
Leucócitos Mononucleares/efeitos dos fármacos
Mycobacterium bovis
Mitógenos/farmacologia
Estadiamento de Neoplasias
Estatísticas não Paramétricas
Limites: Adolescente
Adulto
Criança
Feminino
Seres Humanos
Masculino
Adulto Jovem
Responsável: BR1.1 - BIREME


  6 / 35 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Castro, Carlos
Texto completo
Id: lil-616850
Autor: Mejía, Wilson; Castro, Carlos; Umaña, Adriana; Clemencia de Castro; Riveros, Tulia; Sánchez-Gómez, Myriam.
Título: Señalización asociada al receptor del factor de crecimiento similar a la insulina de tipo I en una línea celular colombiana de carcinoma mamario / Insulin-like growth factor receptor I signaling in a breast cancer cell line
Fonte: Biomédica (Bogotá);30(4):551-558, dic. 2010. ilus.
Idioma: es.
Resumo: Introducción. Varios estudios in vitro sugieren que la proliferación, migración y supervivencia en líneas celulares de cáncer de seno se ven significativamente afectadas por la activación del receptor IGF de tipo I (Insulin-like Growth Factor-I Receptor, IGF-IR).Objetivo. Caracterizar la fosforilación del IGF-IR y las moléculas de señalización intracelular Akt y Erk1/2 (vías de señalización PI-3K y MAPK, respectivamente), causada por el factor IGF-I en una línea celular colombiana de cáncer de mama ductal infiltrante (CSC 1595). Materiales y métodos. Las líneas celulares CSC 1595 y MCF-7 se cultivaron en medio DMEM con suplemento de suero fetal bovino 10%, glutamina 2 mM, penicilina 100 U/ml, estreptomicina 100 µg/ml a 37 0C, atmósfera de CO2 al 5% y humedad de 95%. Los extractos celulares se sometieron a inmunoprecipitación e inmunodetección con anticuerpos específicos anti-pIGF-IR, anti-pERK1/2 y anti-pAkt. Resultados. Cinco minutos después del estímulo con 10 nM de IGF-I, 70% y 25% del IGF-IR fueron fosforilados en las células CSC 1595 y MCF-7, respectivamente; también se observó activación de las proteínas Akt y ERK1/2. Los niveles basales y estimulados de las proteínas ERK1/2 fueron significativamente más altos en las células CSC 1595, comparados a los observados en MCF-7. Conclusiones. El IGF-IR y la vía MAPK cinasa que involucra las proteínas ERK1/2 muestran una fosforilación mayor en las células 1595 a la observada en las MCF-7. El IGF-IR, principal activador de esta vía, podría jugar un papel importante en los procesos de proliferación y metástasis de tumores de cáncer de mama ductal infiltrante.

Introduction. In vitro studies strongly suggest that proliferation, migration and cell survival of breast cancer cell lines are significantly affected by activation of the IGF type 1 receptor (IGF-IR).Objective. The phosphorylation by IGF-I of IGF-IR and the intracellular signaling molecules Akt (PI-3K pathway) and Erk1/2 (MAPK pathway) was characterized in a human breast cancer cell lines. Materials and methods. The study compared a standard breast adenocarcinoma line (MCF-7) cell line with a line (CSC 1595) derived from an infiltrating ductal breast cancer in a Colombian patient. The CSC 1595 and MCF-7 cell lines were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM glutamine, 100 U/ml penicillin, and 100 µg/ml streptomycin and grown at 37°C in 5% CO2 atmosphere and 95% humidity. Cell extracts were prepared, followed by immunoprecipitation and immunoblotting with specific anti-pIGF-IR, anti-pERK1/2 and anti-pAkt antibodies. Results. After 5 minute stimulation with IGF-I, 70% of the IGF-IR was phosphorylated in the cell line CSC 1595 and 25% in MCF-7. In addition, Akt (oncogene protein v-akt) and ERK1/2 (extracellular signal-regulated MAP kinases) were phosphorylated. Basal and stimulated levels of phosphorylated ERK1/2 were substantially higher compared to those in the MCF-7 cell line. Conclusions. The IGF-IR and MAPK kinase pathway involving proteins ERK1/2 showed more significant phosphorylation in the 1,595 cells compared to the observed in the MCF-7 cell line. Since the IGF-IR is the major activator of this pathway it may play an important role in ductal infiltrating breast cancer tumor growth and metastases.
Descritores: Neoplasias da Mama
Linhagem Celular
-Mitógenos
Responsável: CO42.1 - Biblioteca Nacional de Salud José Celestino Mutis


  7 / 35 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-606547
Autor: Ren, Kewei; Ma, Yimin; Huang, Yumin; Liang, Wenwei; Liu, Feng; Wang, Qing; Cui, Weiding; Liu, Zhengyu; Yin, Guoyong; Fan, Weimin.
Título: Periodic mechanical stress activates MEK1/2-ERK1/2 mitogenic signals in rat chondrocytes through Src and PLCγ1
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;44(12):1231-1242, Dec. 2011. ilus, tab.
Idioma: en.
Resumo: The mitogenic effects of periodic mechanical stress on chondrocytes have been studied extensively but the mechanisms whereby chondrocytes sense and respond to periodic mechanical stress remain a matter of debate. We explored the signal transduction pathways of chondrocyte proliferation and matrix synthesis under periodic mechanical stress. In particular, we sought to identify the role of the MEK1/2-ERK1/2 signaling pathway in chondrocyte proliferation and matrix synthesis following cyclic physiologic mechanical compression. Under periodic mechanical stress, both rat chondrocyte proliferation and matrix synthesis were significantly increased (P < 0.05) and were associated with increases in the phosphorylation of Src, PLCγ1, MEK1/2, and ERK1/2 (P < 0.05). Pretreatment with the MEK1/2-ERK1/2 selective inhibitor, PD98059, and shRNA targeted to ERK1/2 reduced periodic mechanical stress-induced chondrocyte proliferation and matrix synthesis (P < 0.05), while the phosphorylation levels of Src-Tyr418 and PLCγ1-Tyr783 were not inhibited. Proliferation, matrix synthesis and phosphorylation of MEK1/2-Ser217/221 and ERK1/2-Thr202/Tyr204 were inhibited after pretreatment with the PLCγ1 inhibitor U73122 in chondrocytes in response to periodic mechanical stress (P < 0.05), while the phosphorylation site of Src-Tyr418 was not affected. Inhibition of Src activity with PP2 and shRNA targeted to Src abrogated chondrocyte proliferation and matrix synthesis (P < 0.05) and attenuated PLCγ1, MEK1/2 and ERK1/2 activation in chondrocytes subjected to periodic mechanical stress (P < 0.05). These findings suggest that periodic mechanical stress promotes chondrocyte proliferation and matrix synthesis in part through the Src-PLCγ1-MEK1/2-ERK1/2 signaling pathway, which links these three important signaling molecules into a mitogenic cascade.
Descritores: Condrócitos/citologia
Condrócitos/enzimologia
Sistema de Sinalização das MAP Quinases/fisiologia
Proteína Quinase 1 Ativada por Mitógeno/metabolismo
Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo
Estresse Mecânico
-Sistema de Sinalização das MAP Quinases/genética
Proteína Quinase 1 Ativada por Mitógeno/genética
Quinases de Proteína Quinase Ativadas por Mitógeno/genética
Mitógenos/metabolismo
Fosfolipase C gama/metabolismo
Ratos Sprague-Dawley
Quinases da Família src/metabolismo
Limites: Animais
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  8 / 35 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Id: lil-506976
Autor: garavito, Zaira; Martinez, Constanza; Hurtado, Hernan.
Título: Proliferación y expresión de marcadores por células de Schwann de rata adulta en cultivo / Schwann cells proliferation and marker expression on adult rat in culture
Fonte: Rev. colomb. biotecnol;2(1):40-49, jul. 1999. ilus, graf.
Idioma: es.
Resumo: En este trabajo se evalúan diferentes técnicas para obten-ción y cultivo de células de Schwann provenientes del nervio periférico de rata adulta, de las cuales la que evi-dencia mejor respuesta es la que combina una degenera-ción walleriana durante 14 días in vitro, seguida de una disociación enzimática.La adición de mitógenos como la forskolina y extracto de pituitaria no muestra un efecto sobre estas células. Los niveles de enriquecimiento en células de Schwann, defi-nidos de acuerdo con patrones morfológicos y de expre-sión de marcadores tales como la proteína S-100 o la pro-teína acida fíbrilar glial (GFAP), son buenos (del orden de 80-88por cien) hasta los ocho días de cultivo. La detección de bromodeoxiouridina (BrdU) incorporada por células en fase S del ciclo celular, demuestra que en términos ge-nerales la tasa de incorporación de BrdU de las células guales del sistema nervioso periférico no cambia.
Descritores: Fármacos do Sistema Nervoso Periférico
Técnicas de Cultura de Células
Mitógenos
Regeneração Nervosa
Células de Schwann
Engenharia Tecidual
Tipo de Publ: Estudos de Avaliação
Estudo Comparativo
Responsável: CO326 - Departamento de Biología


  9 / 35 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-494483
Autor: Ferreira, Ana Paula; Côrrea, Thadeu; Cunha, Rosângela; Marques, Marcos José; Montesano, Maria Angela; Souza, Maria Aparecida; Teixeira, Henrique Couto.
Título: Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate / Reatividade de anticorpos de soros humanos a antígenos de Paracoccidioides brasiliensis tratados com metaperiodato sódico
Fonte: Rev. Soc. Bras. Med. Trop;41(4):325-329, jul.-ago. 2008. ilus, graf.
Idioma: en.
Resumo: In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosis presented higher reactivity towards the whole antigen than to the antigen treated with metaperiodate (P < 0.05). The reactivity of IgG and IgM to the antigen treated with metaperiodate was greater in serum from patients with the acute form of the disease (P < 0.05), while IgA was more reactive in serum from patients with the chronic form (P < 0.05). There was greater reactivity of IgG1 and IgG2 to the whole antigen and the antigen treated with metaperiodate in the serum from patients with paracoccidioidomycosis than there was in serum from patients with other parasitic infections (P < 0.05). Furthermore, IgG1 from patients with the acute form recognized the 19kDa, 27kDa and 31kDa antigens in the western blot test. Thus, the results suggest that modifications to the epitopes of Paracoccidioides brasiliensis antigens may help to improve the immunodiagnosis of paracoccidioidomycosis.

Neste trabalho, foi avaliado o perfil de isotipos de imunoglobulinas anti-Paracoccidioides brasiliensis em soros de pacientes com formas crônica e aguda de paracoccidiodomicoses usando antígeno total e tratado com meta-periodato. Todos os tipos de imunoglobulinas presentes nos soros de pacientes com formas aguda e crônica apresentaram alta reatividade ao antígeno total quando comparado ao tratado com meta-periodato (P < 0,05). Houve maior reatividade de IgG e IgM anti-antígeno tratado com meta-periodato em soros de pacientes com forma aguda da doença (P < 0,05), enquanto IgA foi mais reativa em soros da forma crônica (P < 0,05). Houve maior reatividade de IgG1 e IgG2 com antígeno total e tratado com meta-periodato em soros de pacientes comparados aos com outras parasitoses (P < 0,05). Além disso, IgG1 de pacientes com a forma aguda reconhecem antígenos de 19kDa, 27kDa e 31kDa por western blot. Assim, os resultados sugerem que alterações nos epitopos de antígenos de Paracoccidioides brasiliensis podem auxiliar no aprimoramento do imunodiagnóstico da paracoccidioidomicose.
Descritores: Anticorpos Antifúngicos/imunologia
Antígenos de Fungos/imunologia
Isotipos de Imunoglobulinas/imunologia
Paracoccidioides/imunologia
Paracoccidioidomicose/imunologia
-Doença Aguda
Anticorpos Antifúngicos/sangue
Anticorpos Antifúngicos/efeitos dos fármacos
Reações Antígeno-Anticorpo/efeitos dos fármacos
Reações Antígeno-Anticorpo/imunologia
Antígenos de Fungos/sangue
Antígenos de Fungos/efeitos dos fármacos
Western Blotting
Estudos de Casos e Controles
Doença Crônica
Epitopos/efeitos dos fármacos
Epitopos/imunologia
Isotipos de Imunoglobulinas/sangue
Isotipos de Imunoglobulinas/efeitos dos fármacos
Mitógenos/uso terapêutico
Paracoccidioides/efeitos dos fármacos
Paracoccidioidomicose/sangue
Paracoccidioidomicose/tratamento farmacológico
Ácido Periódico/uso terapêutico
Limites: Seres Humanos
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  10 / 35 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-492884
Autor: Alves, R; Silva, D. A. O; Fernandes, J. F. C; Almeida, K. C; Ynoue, L. H; Bernardes, C. T. V; Moreira, P. F. S; Gennari-Cardoso, M. L; Sung, S. J; Taketomi, E. A.
Título: Humoral and cellular immune responses to Blomia tropicalis and concanavalin A-binding fractions in atopic patients
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;41(9):773-781, Sept. 2008. graf, tab.
Idioma: en.
Projeto: CAPES; . CNPq; . FAPEMIG.
Resumo: Blomia tropicalis, Dermatophagoides pteronyssinus and D. farinae are prevalent house dust mites. Concanavalin A-binding components derived from B. tropicalis (Bt-ConA extract) are highly immunogenic in allergic diseases. The aim of the present study was to evaluate the humoral and cellular immune responses to B. tropicalis in mite-sensitized patients. A total of 137 patients with allergic rhinitis with/without asthma and 109 non-atopic subjects were selected and analyzed by the skin prick test, and for total serum IgE and specific IgE levels to both Bt-total and Bt-ConA extracts, their proliferative response and cytokine (IFN-ã and IL-5) production by peripheral blood mononuclear cells (PBMC) stimulated with both extracts. Skin prick test showed that 70 percent of the patients were sensitized to Bt (Bt+) and similar levels of specific IgE to Bt-total and Bt-ConA extracts were demonstrable in Bt+ patients. Significant PBMC proliferation was observed in response to Bt-total extract in Bt+, but not in Bt- patients and non-atopic subjects (P < 0.001). Bt-ConA extract induced increased proliferative responses in all patient groups compared to medium alone (P < 0.05), but these responses were significantly decreased in the presence of the mannopyranoside ConA inhibitor (P < 0.05). Significant IFN-ã production was observed after Bt-ConA stimulation of Bt+ patients (P < 0.05), while Bt-total extract had no effect. IL-5 production was consistently detected in Bt+ patients after allergen-specific stimulation or with no stimulus, indicating that PBMC from allergic patients are prone to produce Th2 profile cytokines, spontaneously or inductively by allergen restimulation. These data showed that ConA-binding components isolated from B. tropicalis may contain relevant antigens that are involved in both humoral and cellular immune responses. However, without an additional purification procedure to eliminate the residual contamination with...
Descritores: Alérgenos/administração & dosagem
Antígenos de Dermatophagoides/administração & dosagem
Concanavalina A/administração & dosagem
Mitógenos/administração & dosagem
Rinite Alérgica Perene/imunologia
-Alérgenos/imunologia
Antígenos de Dermatophagoides/imunologia
Estudos de Casos e Controles
Proliferação Celular
Concanavalina A/imunologia
Dessensibilização Imunológica
Imunoglobulina E/sangue
Imunoglobulina E/imunologia
Interferon gama/biossíntese
/biossíntese
INTERLEUKIN-ABDOMEN/biossíntese
Leucócitos Mononucleares/imunologia
Ácaros/imunologia
Mitógenos/imunologia
Rinite Alérgica Perene/sangue
Limites: Adulto
Animais
Feminino
Seres Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



página 1 de 4 ir para página            
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde