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Id: |
biblio-841802
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Autor: |
Mello, Cíntia da Silva; Valente, Ligia Maria Marino; Wolff, Thiago; Lima-Junior, Raimundo Sousa; Fialho, Luciana Gomes; Marinho, Cintia Ferreira; Azeredo, Elzinandes Leal; Oliveira-Pinto, Luzia Maria; Pereira, Rita de Cássia Alves; Siani, Antonio Carlos; Kubelka, Claire Fernandes. |
Título: |
Decrease in Dengue virus-2 infection and reduction of cytokine/chemokine production by Uncaria guianensis in human hepatocyte cell line Huh-7 |
Fonte: |
Mem. Inst. Oswaldo Cruz;112(6):458-468, June 2017. tab, graf.
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Idioma: |
en.
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Resumo: |
ABSTRACT BACKGROUND Dengue fever may present hemorrhages and cavitary effusions as result of exacerbated immune responses. We investigated hydro-alcoholic extracts from leaves (UGL) and bark (UGB) of the medicinal species Uncaria guinanensis with respect to antiviral effects in Dengue virus (DENV) infection and in immunological parameters associated with in vivo physiopathological features. METHODS Chemical profiles from UGB or UGL were compared in thin layer chromatography and 1H nuclear magnetic resonance using flavonoid compounds and a pentacyclic oxindole alkaloid-enriched fraction as references. DENV-2-infected hepatocytes (Huh-7) were treated with extracts. Cell viability, DENV antigens and immunological factors were detected by enzyme-linked immunosorbent assay (ELISA) or flow cytometry. FINDINGS The UGL mainly differed from UGB by selectively containing the flavonoid kaempferitrin. UGB and UGL improved hepatocyte viability. Both extracts reduced intracellular viral antigen and inhibited the secretion of viral non-structural protein (NS1), which is indicative of viral replication. Reduction in secretion of macrophage migration inhibitory factor was achieved by UGB, of interleukin-6 by UGL, and of interleukin-8 by both UGB and UGL. MAIN CONCLUSIONS The U. guianensis extracts presented, antiviral and immunomodulatory effects for DENV and possibly a hepatocyte-protective activity. Further studies may be performed to consider these products as potential candidates for the development of an herbal product for the future treatment of dengue.
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Descritores: |
Antivirais/farmacologia Extratos Vegetais/farmacologia Sobrevivência Celular/efeitos dos fármacos Citocinas/efeitos dos fármacos Citocinas/imunologia Quimiocinas/efeitos dos fármacos Quimiocinas/imunologia Uncaria/química Dengue/fisiopatologia Dengue/imunologia Dengue/virologia Vírus da Dengue/efeitos dos fármacos Vírus da Dengue/imunologia Antígenos Virais/efeitos dos fármacos Antígenos Virais/imunologia
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-Ensaio de Imunoadsorção Enzimática Citometria de Fluxo
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Limites: |
Seres Humanos
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Responsável: |
BR1.1 - BIREME
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