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Id: biblio-1132473
Autor: Dantas, L L S F R; Fonseca, A G; Pereira, J R; Furtado, A A; Gomes, P A T M; Fernandes-Pedrosa, M F; Leite, A C L; Rêgo, M J B M; Pitta, M G R; Lemos, T M A M.
Título: Anti-inflammatory and antinociceptive effects of the isatin derivative (Z)-2-(5-chloro-2-oxoindolin-3-ylidene)-N-phenyl-hydrazinecarbothioamide in mice
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;53(10):e10204, 2020. graf.
Idioma: en.
Resumo: Several isatin derivatives have shown important biological activities, which have attracted interest from researchers. For this reason, the present study aimed to evaluate the anti-inflammatory and antinociceptive effects of the isatin derivative (Z)-2-(5-chloro-2-oxoindolin-3-ylidene)-N-phenyl-hydrazinecarbothioamide (COPHCT) in mice. Three doses of this compound were tested: 1.0, 2.5, and 5.0 mg/kg. The anti-inflammatory activity was assessed using the carrageenan-induced paw edema model and the zymosan-induced air pouch model. The evaluation of the antinociceptive effect was performed through the formalin test and the acetic acid-induced abdominal writhing test. The paw edema assay demonstrated that all doses of the compound showed a significant reduction of the edema in the second hour evaluated, but a better response was observed in the fourth hour. The zymosan-induced air pouch model indicated that the compound, in all doses, significantly reduced leukocyte migration and total protein concentration levels. In the formalin test, the doses 1.0, 2.5, and 5.0 mg/kg of COPHCT showed activity only in the second phase, with reduction in paw pain time of 73.61, 79.46, and 73.85%, respectively. The number of abdominal writhings decreased with the increasing dose, but only 5.0 mg/kg COPHCT exhibited a significant response, with a reduction of 24.88%. These results demonstrated the ability of this compound to interfere in the anti-inflammatory activity of edema, vascular permeability, and cell migration. In addition, its possible antinociceptive effect may be related to the dose used.
Descritores: Analgésicos/farmacologia
Isatina/farmacologia
Anti-Inflamatórios/farmacologia
-Extratos Vegetais
Carragenina
Edema
Limites: Animais
Masculino
Feminino
Ratos
Responsável: BR1.1 - BIREME


  2 / 1308 LILACS  
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Id: biblio-902597
Autor: De La Fuente, Marjorie; Chahuán, Isidora; Gutiérrez, RocÍo; Díaz-Jiménez, David; Olivares, Mauricio; Vidal, Roberto; Simian, Daniela; Figueroa, Carolina; Quera, Rodrigo; Hermoso, Marcela A.
Título: Presencia de Escherichia coli intracelular en mucosa intestinal de pacientes con Enfermedad Inflamatoria Intestinal y su asociación con características clínicas y el uso de corticosteroides / Presence of intracellular Escherichia coli in patients with inflammatory bowel disease
Fonte: Rev. méd. Chile;145(9):1129-1136, set. 2017. tab, graf.
Idioma: es.
Projeto: Proyecto Fondecyt; . Proyecto.
Resumo: Background: Different strains of invasive Escherichia coli (E. coli), isolated from intestinal mucosa of patients, are related to the pathogenesis of inflammatory bowel diseases (IBD). Aim: To evaluate an association between intracellular E. coli and IBD; its clinical characteristics and use of steroids. Material and Methods: Sixty one patients with Crohn's disease and 83 with ulcerative colitis were studied. To determine the intracellular E. coli content, colonoscopy biopsies of these patients and 29 control subjects were processed using the gentamicin protection assay. Differences in the bacterial content between patient groups were evaluated using Mann-Whitney test, while the association between presence of E. coli with endoscopic activity, location/extension and use of corticosteroid as anti-inflammatory treatment were evaluated with Fisher's exact test or Chi-square test. Results: E. coli strains were detected in 36.1, 39.3 and 10.3% of patients with ulcerative colitis, Crohn's disease and controls, respectively. The number of bacteria per biopsy in Crohn's disease and ulcerative colitis was significantly higher than in controls (p < 0.01 between patients and controls). In ulcerative colitis, significant associations were found between the presence of bacteria and disease location and use of corticosteroids. In Crohn's disease, no association was found. Conclusions: IBD are associated with the presence of intracellular E. coli strains in the intestinal mucosa, suggesting an alteration in the microbiota or loss of integrity of the epithelial barrier. The association of intracellular E. coli with clinical features and the use of corticosteroids in ulcerative colitis suggests that different factors could promote colonization or proliferation of these bacteria.
Descritores: Colite Ulcerativa/microbiologia
Doença de Crohn/microbiologia
Escherichia coli/isolamento & purificação
Mucosa Intestinal/microbiologia
-Valores de Referência
Contagem de Colônia Microbiana
Colite Ulcerativa/tratamento farmacológico
Doença de Crohn/tratamento farmacológico
Estudos de Casos e Controles
Estudos Prospectivos
Corticosteroides/uso terapêutico
Estatísticas não Paramétricas
Anti-Inflamatórios/uso terapêutico
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1040148
Autor: Haidara, Mohamed A; Al-Hashem, Fahaid; El-Karib, Abbas O; Zaki, Mohamed S; Kamar, Samaa S; El-Bidawy, Mahmoud H; Al-Ani, Bahjat.
Título: Inhibition of paracetamol-induced acute kidney damage in rats using a combination of resveratrol and quercetin / Inhibición de daño renal agudo inducido por paracetamol en ratas usando una combinación de resveratrol y quercetina
Fonte: Int. j. morphol;37(4):1422-1428, Dec. 2019. graf.
Idioma: en.
Projeto: King Khalid University.
Resumo: Paracetamol (also called acetaminophen, or APAP) overdose causes acute damage to the liver and kidneys in both humans and experimental animal models via the induction of the oxidative stress pathway. We sought to determine whether the combined antioxidants and anti-inflammatory compounds, resveratrol (RES) and quercetin (QUR) can protect against kidney injury induced by a toxic dose of APAP in a rat model of APAP-induced acute kidney injury. Rats were either received a single dose of APAP (2 g/kg) before being sacrificed after 24 hours or were pre-treated for 7 days with combined doses of RES (30 mg/kg) and QUR (50 mg/kg) before being given a single dose of APAP and then sacrificed 24 hours post APAP ingestion. Harvested kidney tissues were prepared for light microscopy staining, and tissue samples were assayed for (i) biomarkers of oxidative stress and antioxidant, malondialdehyde (MDA) and superoxide dismutase (SOD); and (ii) biomarkers of inflammation, tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6). Hematoxylin and eosin (H&E) stained images showed that APAP overdose induced acute kidney injury as demonstrated by widening of glomeruli space (Bowman space), tubular dilatation, numerous cellular debris in the renal tubules with tubular epithelial degeneration, and vacuolization, which were effectively protected by RES+QUR except a partial protection of the glomeruli space was observed. In addition, APAP significantly (p<0.05) modulated tissue levels of MDA, SOD, TNF-α, and IL-6, which were protected by RES+QUR. Furthermore, a significant (p<0.0001) positive correlation was observed between glomeruli space and TNF-α, (r=0.8899), IL-6 (r=0.8986), and MDA (r=0.8552), whereas glomeruli space scoring versus SOD showed negative correlation (r= - 0.7870). We conclude that resveratrol plus quercetin substantially protects against APAP-induced acute kidney injury in rats, possibly via the augmentation of antioxidants and inhibition of oxidative stress and inflammation.

La sobredosis de paracetamol (también llamado acetaminofen o APAP) causa un daño agudo en el hígado y los riñones, tanto en humanos como en modelos animales experimentales, a través de la inducción de la vía del estrés oxidativo. Intentamos determinar si los antioxidantes y los compuestos antiinflamatorios combinados, el resveratrol (RES) y la quercetina (QUR) pueden proteger contra la lesión renal inducida por una dosis tóxica de APAP en un modelo de rata de lesión renal aguda inducida por APAP. Las ratas recibieron una dosis única de APAP (2 g / kg) antes de ser sacrificadas después de 24 horas o se trataron previamente durante 7 días con dosis combinadas de RES (30 mg / kg) y QUR (50 mg / kg), antes de ser tratadas, se administró una dosis única de APAP y luego fueron sacrificadas 24 horas después de la ingestión. Los tejidos renales recolectados se tiñeron con H-E y fueron observados a través de microscopía óptica. Las muestras de tejido se analizaron para (i) biomarcadores de estrés oxidativo y antioxidante, malondialdehído (MDA) y superóxido dismutasa (SOD); y (ii) biomarcadores de inflamación, factor de necrosis tumoral alfa (TNF-α) e interleucina-6 (IL-6). Las imágenes teñidas con H & E mostraron que la sobredosis de APAP indujo daño renal agudo como lo demuestra la ampliación del espacio glomerular, la dilatación tubular, numerosos desechos celulares en los túbulos renales con degeneración epitelial tubular y la vacuolización, que se protegieron eficazmente con RES + QUR Se observó una protección parcial del espacio glomerular. Además, APAP modificó significativamente (p <0.05) los niveles tisulares de MDA, SOD, TNF-α e IL-6, que estaban protegidos por RES + QUR. Además, se observó una correlación positiva significativa (p <0,0001) entre el espacio glomerular y el TNF-α, (r = 0,8899), IL-6 (r = 0,8986) y MDA (r = 0,8552), mientras que la puntuación del espacio glomerular versus SOD mostró correlación negativa (r = - 0,7870). Concluimos que el resveratrol más quercetina protege sustancialmente contra la lesión renal aguda inducida por APAP en ratas, posiblemente a través del aumento de antioxidantes y la inhibición del estrés oxidativo y la inflamación.
Descritores: Quercetina/uso terapêutico
Injúria Renal Aguda/tratamento farmacológico
Resveratrol/uso terapêutico
Acetaminofen/toxicidade
-Quercetina/farmacologia
Estresse Oxidativo/efeitos dos fármacos
Modelos Animais de Doenças
Quimioterapia Combinada
Injúria Renal Aguda/induzido quimicamente
Resveratrol/farmacologia
Acetaminofen/antagonistas & inibidores
Anti-Inflamatórios/uso terapêutico
Antioxidantes/uso terapêutico
Limites: Animais
Ratos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1004361
Autor: Contreras-Olea, Oscar; Goecke-Hochberger, Carola; Rumié-Carmi, Hana Karime; Lobo-Avilés, Rosendo; Mellado-Sagredo, Cecilia; Avila-Smirnow, Daniela.
Título: Fibrodisplasia osificante progresiva plus por una variante patogénica del gen ACVR1: caso clínico / Fibrodysplasia ossificans progressiva: report of one case
Fonte: Rev. méd. Chile;147(3):384-389, mar. 2019. tab, graf.
Idioma: es.
Resumo: Fibrodysplasia ossificans progressiva (FOP) or myositis ossificans, is a genetic disease, with a prevalence of 1 in 2.000.000. It is caused by pathogenic variants in ACVR1 gene and characterized by soft tissue heterotopic ossification, starting in the second decade of life. It is associated to early mortality caused by respiratory complications. It evolves in flare-ups, triggered by soft tissue injuries; therapy is symptomatic, using analgesia, steroids and diphosphonates. We report a 12-year-old female with left renal agenesis, hallux valgus and intellectual disability, presenting with a six months history of thoracic kyphosis, tender nodules in the thorax, and rigidity of right elbow and left knee. Clinical examination revealed dysmorphic facial features. A magnetic resonance showed heterotopic ossification nodules, which was confirmed with spinal radiography. These findings prompted the diagnosis of FOP. Pain treatment was started, and prednisone was used during flare-ups. The ACVR1 gene was analyzed and a pathogenic variant, p. Arg206His, was found, confirming the diagnosis of FOP.
Descritores: Miosite Ossificante/diagnóstico por imagem
-Prednisona/uso terapêutico
Imageamento por Ressonância Magnética
Chile
Ossificação Heterotópica/genética
Ossificação Heterotópica/tratamento farmacológico
Ossificação Heterotópica/diagnóstico por imagem
Anti-Inflamatórios/uso terapêutico
Miosite Ossificante/genética
Miosite Ossificante/tratamento farmacológico
Limites: Humanos
Feminino
Criança
Tipo de Publ: Relatos de Casos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1132547
Autor: Yang, Zheng; Li, Miaojuan; Xiao, Lin; Yi, Zhiming; Zhao, Min; Ma, Shengjie.
Título: Hyaluronic acid versus dexamethasone for the treatment of recurrent aphthous stomatitis in children: efficacy and safety analysis
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;53(8):e9886, 2020. tab.
Idioma: en.
Resumo: The objective of this study was to compare the safety and efficacy of 0.2% hyaluronic acid (HA) topical gel and dexamethasone topical ointment in the treatment of recurrent aphthous ulcers (RAU) in children. This retrospective observational study included 104 patients who had more than two episodes of oral aphthous ulcers per year and were treated with HA (n=52) or dexamethasone (n=52) from August 15, 2014 to September 3, 2018. Therapy efficacy was evaluated based on the ulcer size and pain score before versus 7 days after either therapy. The paired t-test, chi-squared test, and independent t-test were utilized for statistical analyses. There was no significant difference in ulcer size or pain score between the HA and dexamethasone groups, on day 1 or day 7. Both treatments were tolerated well and no side effects were reported. No significant differences in body temperature, respiration rate, pulse, or systolic/diastolic blood pressure were observed between the start (day 1) and end of treatment (day 7), for either treatment. HA and dexamethasone showed similar efficacy in reducing ulcer size and pain scores, and were tolerated equally well in children with RAU. Future high-quality studies with larger numbers of patients are needed to confirm our findings.
Descritores: Estomatite Aftosa/tratamento farmacológico
Dexametasona/uso terapêutico
Adjuvantes Imunológicos/uso terapêutico
Ácido Hialurônico/uso terapêutico
Anti-Inflamatórios/uso terapêutico
-Dor
Recidiva
Estudos Retrospectivos
Limites: Humanos
Masculino
Feminino
Criança
Responsável: BR1.1 - BIREME


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Id: biblio-1132555
Autor: Foschetti, D A; Braga-Neto, M B; Bolick, D; Moore, J; Alves, LA; Martins, CS; Bomfin, LE; Santos, AAQA; Leitão, RFC; Brito, GAC; Warren, CA.
Título: Clostridium difficile toxins or infection induce upregulation of adenosine receptors and IL-6 with early pro-inflammatory and late anti-inflammatory pattern
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;53(9):e9877, 2020. tab, graf.
Idioma: en.
Projeto: PRONEX/FUNCAP/CNPq.
Resumo: Clostridium difficile causes intestinal inflammation, which increases adenosine. We compared the expression of adenosine receptors (AR) subtypes A1, A2A, A2B, and A3 in HCT-8, IEC-6 cells, and isolated intestinal epithelial cells, challenged or not with Clostridium difficile toxin A and B (TcdA and TcdB) or infection (CDI). In HCT-8, TcdB induced an early A2BR expression at 6 h and a late A2AR expression at 6 and 24 h. In addition, both TcdA and TcdB increased IL-6 expression at all time-points (peak at 6 h) and PSB603, an A2BR antagonist, decreased IL-6 expression and production. In isolated cecum epithelial cells, TcdA induced an early expression of A2BR at 2s and 6 h, followed by a late expression of A2AR at 6 and 24 h and of A1R at 24 h. In CDI, A2AR and A2BR expressions were increased at day 3, but not at day 7. ARs play a role in regulating inflammation during CDI by inducing an early pro-inflammatory and a late anti-inflammatory response. The timing of interventions with AR antagonist or agonists may be of relevance in treatment of CDI.
Descritores: Toxinas Bacterianas
Clostridioides difficile
Infecções por Clostridium
Receptores Purinérgicos P1/metabolismo
-Proteínas de Bactérias
Regulação para Cima
Interleucina-6
Modelos Animais de Doenças
Enterotoxinas
Infecções
Anti-Inflamatórios
Limites: Animais
Responsável: BR1.1 - BIREME


  7 / 1308 LILACS  
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Id: lil-700585
Autor: MACHADO-ALBA, JORGE; MACHADO-DUQUE, MANUEL; CALDERÓN, VIVIANA; GONZÁLEZ, ALEXANDRA; CARDONA, FELIPE; RUIZ, RICHARD; MONTOYA, JULIÁN.
Título: Control del dolrr postquirúrgico en pacientes de un hospital de tercer nivel / Conrol of postopperative pain in patients in a tertiary hospital / Conrole da dor ppós-cirúrgica em pacientes de um hospital de terceiro níeel
Fonte: Rev. MED;21(1):46-53, ene.-jun. 2013. ilus, tab.
Idioma: es.
Resumo: Introducción: el manejo del dolor debe ser adaptado y optimizado de acuerdo con las condiciones de cada hospital, tipo de cirugía y paciente. Objetivos: ealuar la percepción del dolor de los pacientes intervenidos en el postoperatorio del Hospital Universitario San Jorge de Pereira. Materiales y métodos: Se realizó un estudio de corte, prospectivo en pacientes mayores de 18 años entre 2 de septiembre y 28 de octubre de 2011. La valoración de intensidad del dolor postoperatorio se realizó mediante Escala Visual Analógica a las 24 horas del postquirúrgico. Se consideraron variables sociodemográficas, clínicas (tipo de cirugía, anestesia, riesgo) y farmacológicas (medicamentos empleados, dosis, intervalos dosificación). El análisis se hizo con SPSS 20.0 para Windows. Resultados: Se evaluaron 153 pacientes en postoperatorio, 80 (52,3%) mujeres y 73 (47,7%) hombres, con edad promedio de 47,6 ± 20,2 años. El 38,8% de los pacientes no tenía controlado el dolor. Las variables de cirugía ortopédica, el empleo de anestesia general por vía intravenosa, y el incumplimiento de los intervalos recomendados de dosificación de los analgésicos, se asociaron de manera estadísticamente significativa con la falta de control. Discusión: El control del dolor fue inadecuado haciendo evidente la necesidad de replantear su manejo ajustado a guías de práctica clínica, formalizando el uso de medicamentos, en dosis e intervalos adecuados que garanticen una analgesia efectiva.

Introduction: Pain management should be adapted and optimized according to the conditions of each hospital, type of surgery and patient. Objectives: To evaluate the perception of pain in postoperative patients at Hospital Universitario San Jorge of Pereira. Materials and methods: A descriptive observational study in patients older than 18 years, between September 2nd to October 28, 2011. The assessment of postoperative pain intensity was performed by visual analogue scale 24 hours after surgery. We considered socio-demographic, clinical (type of surgery, anesthesia, risk) and pharmacological variables (drugs used, dosage, dosage intervals). The analysis was done with SPSS 20.0 for Windows. Results: We evaluated 153 patients in the postoperative period, 80 (52.3%) women and 73 (47.7%) males, mean age 47.6 ± 20.2 years. 38.8% of patients did not obtain pain control. The variables of orthopedic surgery, the use of intravenous general anesthesia, and suboptimal dosage was statistically significantly associated with lack of control. Conclusions: Pain control was inadequate thus showing the need to redefine its management by following clinical practice guidelines, through the use of analgesics in doses and appropriate intervals.

Introdução: o manejo da dor deve ser adaptado e otimizado de acordo com as condições de cada hospital, tipo de cirurgia e paciente. Objetivos: avaliar a percepção da dor dos pacientes intervindos no pós operatório do Hospital Universitário San Jorge de Pereira. Materiais e métodos: Realizou-se um estudo de corte, prospectivo em pacientes maiores de 18 anos entre 2 de setembro e 28 de outubro de 2011. A valoração de intensidade da dor pós-operatória se realizou mediante Escala Visual Analógica às 24 horas do pós-cirúrgico. Consideraram-se variáveis sociodemográficas, clínicas (tipo de cirurgia, anestesia, risco) e farmacológicas (medicamentos empregados, doses, intervalos, dosagem). A análise se fez com SPSS 20.0 para Windows. Resultados: Se avaliaram 153 pacientes em pós-operatório, 80 (52,3%) mulheres e 73 (47,7%) homens, com idade média de 47,6 ± 20,2 anos. O 38,8% dos pacientes não tinha controlada a dor. As variáveis de cirurgia ortopédica, o emprego de anestesia geral por via intravenosa, e o descumprimento dos intervalos recomendados de dosagem dos analgésicos, se associaram de maneira estatisticamente significativa com a falta de controle. Discussão: O controle da dor foi inadequado fazendo evidente a necessidade de repensar o seu manejo ajustado a guias de prática clínica, formalizando o uso de medicamentos, em doses e intervalos adequados que garantam uma analgesia efetiva.
Descritores: Dor Pós-Operatória
-Guia de Prática Clínica
Colômbia
Analgésicos Opioides
Anti-Inflamatórios
Limites: Humanos
Adulto
Tipo de Publ: Artigo Clássico
Responsável: CO87.1 - Biblioteca Médica


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Id: biblio-1249321
Autor: Sun, Y D; Gao, Y S; Xu, L W; Zhang, Y F; Cheng, C; Wei, K C; Lin, Jian; Chen, G; Liu, C Y; Li, Q F.
Título: Protective effects of sodium ferulate on flap transplantation via anti-inflammatory modulation and oxidative stress inhibition
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;54(7):e10520, 2021. graf.
Idioma: en.
Projeto: Science and Technology Committee of Shanghai Municipal Government; . National Natural Science Foundation of China; . China Postdoctoral Science Foundation.
Resumo: Ischemia-reperfusion injury (IRI) has brought attention to flap failure in reconstructive surgery. To improve the prognosis of skin transplantation, we performed experimental IRI by surgical obstruction of blood flow and used sodium ferulate (SF) to prevent IRI in rats. After SF treatment, the morphological and histological changes of the skin flaps were observed by H&E and Masson's trichrome staining. We also detected the expression levels of COX-1, HO-1, and Ki67 by immunohistochemical and western blot analysis. Moreover, enzyme-linked immunosorbent assay was used to identify the content of tumor necrosis factor (TNF)-α, myeloperoxidase (MPO), malondialdehyde (MDA), and nitric oxide (NO) in peripheral blood and skin tissue. Compared with the model group, SF treatment significantly improved the recovered flap area (%) and promoted collagen synthesis. Cyclooxygenase-2 (COX-2) expression was significantly inhibited by heme oxygenase-1 (HO-1) induction after SF treatment. Furthermore, SF significantly inhibited the levels of TNF-α in peripheral blood, MPO and MDA in the skin tissue, and the increased synthesis of NO. Our results showed the protective effects of SF on IRI after flap transplantation and we believe that the protective effects of SF was closely related to the alleviation of the inflammatory response and the inhibition of the oxidative stress injury.
Descritores: Traumatismo por Reperfusão/prevenção & controle
Traumatismo por Reperfusão/tratamento farmacológico
Estresse Oxidativo
-Ácidos Cumáricos/farmacologia
Anti-Inflamatórios/farmacologia
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


  9 / 1308 LILACS  
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Id: biblio-1249329
Autor: Key Laboratory of Tropical Translational Medicine of Ministry of EducationRen, Shouzhong; Key Laboratory of Tropical Translational Medicine of Ministry of EducationChen, Bangpei; Key Laboratory of Tropical Translational Medicine of Ministry of EducationMa, Zhijian; Hu, Hui; Xie, Yiqiang.
Título: Polygonum hydropiper extract attenuates ethanol-induced gastric damage through antioxidant and anti-inflammatory pathways
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;54(8):e10841, 2021. graf.
Idioma: en.
Projeto: Natural Science Foundation of Hainan Province; . National Natural Science Foundation of China.
Resumo: The present study was conducted to investigate the underlying mechanisms and effective components of Polygonum hydropiper in ethanol-induced acute gastric mucosal lesions. The ethanol extract was purified on an AB-8 macroporous resin column and eluted with 60% ethanol and was then injected into the HPLC system for quantitative analysis. Sprague-Dawley rats were orally pretreated with P. hydropiper extract (PHLE; 50, 100, and 200 mg/kg) for 5 days and then absolute ethanol was administered to induce gastric mucosal damage. One hour after ethanol ingestion, the rats were euthanized and stomach samples were collected for biochemical analysis. Antioxidant enzymes and anti-inflammatory cytokines were quantified. Western blotting was used to detect the expression levels of proteins. Cell proliferation was assayed by CCK-8 assays. The proportion of total flavonoids in the final extract of P. hydropiper was 50.05%, which contained three major bioactive flavonoid constituents, including rutin, quercitrin, and quercetin. PHLE significantly increased cell viability and effectively protected human gastric epithelial cells-1 against alcohol-induced damage in vitro. PHLE pretreatment attenuated gastric mucosal injuries in a dose-dependent manner in rats, and increased the activity of superoxide dismutase, glutathione peroxidase, and glutathione, and decreased the levels of malondialdehyde in gastric tissue. Pretreatment with PHLE also reduced the generation of the pro-inflammatory cytokines tumor necrosis factor-α and interleukin-1β in gastric tissue by downregulating the expression of nuclear factor-kappa B. PHLE exerted protective effects against gastric injury through antioxidant and anti-inflammatory pathways. Flavonoids might be the main effective components of P. hydropiper against gastric mucosal injury.
Descritores: Polygonum
Antioxidantes/farmacologia
-Extratos Vegetais/farmacologia
Ratos Sprague-Dawley
Etanol/toxicidade
Mucosa Gástrica
Anti-Inflamatórios/farmacologia
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-1249333
Autor: Al-Muzafar, H M; Alshehri, F S; Amin, K A.
Título: The role of pioglitazone in antioxidant, anti-inflammatory, and insulin sensitivity in a high fat-carbohydrate diet-induced rat model of insulin resistance
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;54(8):e10782, 2021. tab, graf.
Idioma: en.
Resumo: We explored the cascade effects of a high fat-carbohydrate diet (HFCD) and pioglitazone (an anti-diabetic therapy used to treat type 2 diabetes mellitus (T2DM)) on lipid profiles, oxidative stress/antioxidant, insulin, and inflammatory biomarkers in a rat model of insulin resistance. Sixty albino rats (80-90 g) were randomly divided into three dietary groups; 1) standard diet; 2) HFCD diet for 12 weeks to induce an in vivo model of insulin resistance; and 3) HFCD diet plus pioglitazone. Blood and tissue samples were taken to assess hepatic function, lipid profiles, oxidative biomarkers, malondialdehyde (MDA) levels, antioxidant defense biomarkers, including reduced glutathione (GSH), superoxide dismutase (SOD), and the inflammatory markers interleukin-6 (IL-6) and tumor necrotic factor (TNF-α). HFCD-fed rats had significantly (P≤0.05) increased serum triacylglycerol (TG), total cholesterol (TC), low-density lipoprotein (LDL), alanine transaminase (ALT), and bilirubin levels, but decreased high-density lipoprotein (HDL) levels compared with the normal group. Moreover, serum leptin, resistin, TNF-α, and IL-6 levels were increased significantly in HFCD animals compared with controls. Similarly, HFCD-induced insulin resistance caused antioxidant and cytokine disturbances, which are important therapy targets for pioglitazone. Importantly, administration of this drug ameliorated these changes, normalized leptin and resistin and inflammatory markers by reducing TNF-α levels. Metabolic cascades of elevated lipid profiles, oxidative stress, insulin, and inflammatory biomarkers are implicated in insulin resistance progression. HFCD induced metabolic cascades comprising hypertriglyceridemia, hyperglycemia, insulin resistance, obesity-associated hormones, and inflammatory biomarkers may be alleviated using pioglitazone.
Descritores: Resistência à Insulina
Diabetes Mellitus Tipo 2/metabolismo
Diabetes Mellitus Tipo 2/tratamento farmacológico
-Carboidratos/farmacologia
Estresse Oxidativo
Dieta Hiperlipídica
Pioglitazona/metabolismo
Pioglitazona/farmacologia
Insulina/metabolismo
Fígado/metabolismo
Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME



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