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Id: biblio-1055384
Autor: Justus, Barbara; Arana, Andrés Fernando Montenegro; Gonçalves, Melissa Marques; Wohnrath, Karen; Boscardin, Patrícia Mathias Döll; Kanunfre, Carla Cristine; Budel, Jane Manfron; Farago, Paulo Vitor; Paula, Josiane de Fátima Padilha de.
Título: Characterization and Cytotoxic Evaluation of Silver and Gold Nanoparticles Produced with Aqueous Extract of Lavandula dentata L. in Relation to K-562 Cell Line
Fonte: Braz. arch. biol. technol;62:e19180731, 2019. graf.
Idioma: en.
Projeto: CAPES.
Resumo: Abstract Metallic nanoparticles have great potential as a chemotherapeutic agent. The aim of this study was to develop and characterize silver and gold nanoparticles using a simple method, as well as evaluating the potential cytotoxic activity in relation to the K-562 cell line. For the synthesis, a solution containing the metallic ions was subjected to magnetic stirring with the aqueous extract of Lavandula dentata L. and a change of colour was observed. With the data obtained from the analyses we concluded that the nanoparticles were successfully obtained by a simple and green method using the aqueous extract of L. dentata. The obtained nanoparticles presented a reduced size, a low level of polydispersion, and a homogenous spherical shape. The nanoparticles presented intense and characteristic diffraction peaks, which could be correlated to the planes of the centred cubic structure of the silver and gold. The two formulations presented predominantly crystalline characteristics. The infrared analysis suggested that the amides and alcohols present in the samples may have been responsible for the reduction and limitation of the size and dispersion of the silver and gold nanoparticles. The cytotoxic assay showed that the nanoparticles demonstrated great potential to reduce the cell viability of the K-562 cell line, especially the gold nanoparticles.
Descritores: Leucemia Mieloide
Lamiaceae/toxicidade
Citotoxinas
Nanopartículas Metálicas/análise
Responsável: BR1.1 - BIREME


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Id: biblio-828664
Autor: Alves, Celha Borges Costa; Segurado, Márcio Nunes; Dorta, Miriam Cristina Leandro; Dias, Fátima Ribeiro; Lenza, Maurício Guilherme; Lenza, Marcos Augusto.
Título: Evaluation of cytotoxicity and corrosion resistance of orthodontic mini-implants
Fonte: Dental press j. orthod. (Impr.);21(5):39-46, Sept.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT Objective: To evaluate and compare in vitro cytotoxicity and corrosion resistance of mini-implants from three different commercial brands used for orthodontic anchorage. Methods: Six mini-implants (Conexão(tm), Neodent(tm) and SIN(tm)) were separately immersed in artificial saliva (pH 6.76) for 30 and 60 days. The cytotoxicity of the corrosion extracts was assessed in L929 cell cultures using the violet crystal and MTT assays, as well as cell morphology under light microscopy. Metal surface characteristics before and after immersion in artificial saliva were assessed by means of scanning electron microscopy (SEM). The samples underwent atomic absorption spectrophotometry to determine the concentrations of aluminum and vanadium ions, constituent elements of the alloy that present potential toxicity. For statistical analysis, one-way ANOVA/Bonferroni tests were used for comparisons among groups with p < 0.05 considered significant. Statistical analysis was carried out with Graph Pad PRISM software Version 4.0. Results: No changes in cell viability or morphology were observed. Mini-implants SEM images revealed smooth surfaces with no obvious traces of corrosion. The extracts assessed by means of atomic absorption spectrophotometry presented concentrations of aluminum and vanadium ions below 1.0 µg/mL and 0.5 µg/mL, respectively. Conclusion: Orthodontic mini-implants manufactured by Conexão(tm), Neodent(tm) and SIN(tm) present high corrosion resistance and are not cytotoxic.

RESUMO Objetivo: avaliar, in vitro, e comparar a citotoxicidade e a resistência à corrosão de mini-implantes de três marcas comerciais diferentes, utilizados para ancoragem ortodôntica. Métodos: seis mini-implantes fabricados pelas empresas Conexão(r), Neodent(r) e SIN(r) foram imersos, separadamente, em saliva artificial (pH = 6,76), por 30 e 60 dias, de forma a obter os extratos da corrosão. A citotoxicidade dos extratos foi avaliada em cultura de células L929, empregando-se a análise de ensaios do cristal violeta e MTT, bem como da morfologia celular sob microscopia óptica. As características da superfície do metal antes e após a imersão em saliva artificial foram avaliadas usando microscopia eletrônica de varredura. Os extratos foram submetidos a espectrofotometria de absorção atômica, para determinar as concentrações dos íons alumínio e vanádio, elementos constituintes da liga e que apresentam toxicidade em potencial. Para análise estatística, os testes one-way ANOVA/Bonferroni foram usados para comparação entre os grupos, com p < 0,05 sendo considerado significativo. A análise estatística foi realizada com o programa Graph Pad PRISM v. 4.0. Resultados: não foi observada alteração na viabilidade ou morfologia celular após a exposição dos mini-implantes aos extratos. A análise dos mini-implantes por microscopia eletrônica de varredura revelou superfícies lisas e sem traços evidentes de corrosão. Os extratos analisados usando espectrofotometria de absorção atômica apresentaram concentrações de íons alumínio e vanádio inferiores a 1,0 µg/ml e 0,5 µg/ml, respectivamente. Conclusão: os mini-implantes fabricados pelas empresas Conexão(r), Neodent(r) e SIN(r) apresentam alta resistência à corrosão e não são citotóxicos.
Descritores: Aparelhos Ortodônticos/efeitos adversos
Implantes Dentários/efeitos adversos
-Propriedades de Superfície
Vanádio/análise
Microscopia Eletrônica de Varredura
Análise de Variância
Desenho de Aparelho Ortodôntico
Corrosão
Citotoxinas/análise
Alumínio/análise
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: biblio-981052
Autor: Medina-Rivera, María Luisa.
Título: ¿Es la aplicación de quimioterapia un procedimiento exento de riesgos? / Is the chemotherapy application a risk-free procedure?
Fonte: Rev. enferm. Inst. Mex. Seguro Soc;2(1):9-11, Enero.-Abr. 1989. tab.
Idioma: es.
Resumo: El uso de agentes citotóxicos se ha descrito con una serie de múltiples efectos tóxicos en las pacientes. Sin embargo, en forma reciente se han documentado efectos tóxicos en el personal, que como enfermeras, prepara y aplica estos medicamentos. Por tal motivo se efectuó un estudio observacional en personal de enfermería que prepara estos medicamentos. En 3,044 procedimientos el personal de enfermería cometió un 36% de errores en la forma en que se deben preparar los antineoplásicos y el 6% de los mismos hubo una exposición accidental a dichos medicamentos. Se analizan las recomendaciones que sobre el manejo de drogas antineoplásicas se han establecido, haciéndose necesario una mayor difusión con el fin de evitar la aparición de efectos secúndanos en este personal.

Data of patients undergoing cytotoxic treatment have been associated with multiple secondary effects. However, some of this side effects had been observed in nurses and pharmacist who handling cytotoxic agents. We report a clinical observation of personnel who worked with this drugs. During 3044 procedures, 36% of nurses had mistakes during procedures and 6% had accidental exposure to this agents. The guidelines and rules for handling antineoplastic. agents are analized. Recommendations for their use are necessary to avoid late side effects in this personnel.
Descritores: Riscos Ocupacionais
Indicadores Básicos de Saúde
Contaminação Química
Exposição a Produtos Químicos
Citotoxinas
Tratamento Farmacológico
Toxicidade
Conduta do Tratamento Medicamentoso
Estudo Observacional
Recursos Humanos de Enfermagem
Recursos Humanos de Enfermagem no Hospital
-México
Limites: Humanos
Tipo de Publ: Editorial
Responsável: MX346.1 - CENAIDS - Centro Nacional de Investigación Documental en Salud


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Id: biblio-950716
Autor: Hung, Tran Manh; Dang, Nguyen Hai; Dat, Nguyen Tien.
Título: Methanol extract from Vietnamese Caesalpinia sappan induces apoptosis in HeLa cells
Fonte: Biol. Res;47:1-5, 2014. ilus, graf, tab.
Idioma: en.
Projeto: Vietnam National Foundation for Science and Technology Development.
Resumo: BACKGROUND: This study evaluated the cytotoxic activity of extracts from Caesalpinia sappan heartwood against multiple cancer cell lines using an MTT cell viability assay. The cell death though induction of apoptosis was as indicated by DNA fragmentation and caspase-3 enzyme activation. RESULTS: A methanol extract from C. sappan (MECS) showed cytotoxic activity against several of the cancer cell lines. The most potent activity exhibited by the MECS was against HeLa cells with an IC50 value of 26.5 ± 3.2 µg/mL. Treatment of HeLa cells with various MECS concentrations resulted in growth inhibition and induction of apoptosis, as indicated by DNA fragmentation and caspase-3 enzyme activation. CONCLUSION: This study is the first report of the anticancer properties of the heartwood of C. sappan native to Vietnam. Our findings demonstrate that C. sappan heartwood may have beneficial applications in the field of anticancer drug discovery.
Descritores: Extratos Vegetais/farmacologia
Apoptose
Caesalpinia/química
Feixe Vascular de Plantas/metabolismo
Antineoplásicos Fitogênicos/farmacologia
-Sais de Tetrazólio
Vietnã
Ensaios de Seleção de Medicamentos Antitumorais/métodos
Células HeLa
Sobrevivência Celular
Concentração Inibidora 50
Citotoxinas/farmacologia
Linhagem Celular Tumoral
Proliferação de Células/efeitos dos fármacos
Metanol
Ativação Enzimática/efeitos dos fármacos
Caspase 3/metabolismo
Fragmentação do DNA
Formazans
Inibidores do Crescimento/farmacologia
Indicadores e Reagentes
Camundongos Endogâmicos C57BL
Antineoplásicos Fitogênicos/isolamento & purificação
Limites: Humanos
Animais
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950728
Autor: Castro, Jenny P; Ocampo, Yanet C; Franco, Luis A.
Título: In vivo and in vitro anti-inflammatory activity of Cryptostegia grandiflora Roxb. ex R. Br. leaves
Fonte: Biol. Res;47:1-8, 2014. ilus, graf, tab.
Idioma: en.
Projeto: University of Cartagena.
Resumo: BACKGROUND: Despite Cryptostegia grandiflora Roxb. ex R. Br. (Apocynaceae) leaves are widely used in folk Caribbean Colombian medicine for their anti-inflammatory effects, there are no studies that support this traditional use. Therefore, this work aimed to evaluate the effect of the total extract and primary fractions obtained from Cryptostegia grandiflora leaves, using in vivo and in vitromodels of inflammation, and further get new insights on the mechanisms involved in this activity. RESULTS: Ethanolic extract of Cryptostegia grandiflora leaves, and its corresponding ether and dichloromethane fractions, significantly reduced inflammation and myeloperoxidase activity (MPO) in ear tissue of mice treated with 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Histological analysis revealed a reduction of edema and leukocyte infiltration. Complementarily, we demonstrated that extract and fractions reduced nitric oxide (NO•) and prostaglandin E2 (PGE2) production in LPS-stimulated RAW 264.7 macrophages, as well as scavenging activity on DPPH and ABTS radicals. CONCLUSIONS: Our results demonstrated for the first time the anti-inflammatory activity of Cryptostegia grandiflora leaves, supporting its traditional use. This activity was related to inhibition of MPO activity, and PGE2 and NO• production. These mechanisms and its antioxidant activity could contribute, at least in part, to the anti-inflammatory effect showed by this plant.
Descritores: Extratos Vegetais/uso terapêutico
Apocynaceae/química
Edema/tratamento farmacológico
Macrófagos/efeitos dos fármacos
Anti-Inflamatórios/farmacologia
-Ocitócicos/análise
Dinoprostona/análise
Peroxidase/antagonistas & inibidores
Folhas de Planta/química
Citotoxinas/farmacologia
Linhagem Celular Tumoral/efeitos dos fármacos
Inflamação/tratamento farmacológico
Camundongos Endogâmicos ICR
Óxido Nítrico/análise
Limites: Animais
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950741
Autor: Hossain, Mohammad Mobarak; Ahamed, Sayed Koushik; Dewan, Syed Masudur Rahman; Hassan, Md Mahadi; Istiaq, Arif; Islam, Mohammad Safiqul; Moghal, Md Mizanur Rahman.
Título: In vivo antipyretic, antiemetic, in vitro membrane stabilization, antimicrobial, and cytotoxic activities of different extracts from Spilanthes paniculata leaves
Fonte: Biol. Res;47:1-9, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: The study was conducted to evaluate the in vitro antimicrobial activity, cytotoxic, and membrane stabilization activities, and in vivo antiemetic and antipyretic potentials of ethanolic extract, n-hexane and ethyl acetate soluble fractions of Spilanthes paniculata leaves for the first time widely used in the traditional treatments in Bangladesh. RESULTS: In antipyretic activity assay, a significant reduction (P < 0.05) was observed in the temperature in the mice tested. At dose 400 mg/kg-body weight, the n-hexane soluble fraction showed the effect (36.7 ± 0.63°C ) as like as the standard (dose 150 mg/kg-body weight) after 5 h of administration. Extracts showed significant (P < 0.001) potential when tested for the antiemetic activity compared to the standard, metoclopramide. At dose 50 mg/kg-body weight, the standard showed 67.23% inhibition, whereas n-hexane and ethyl acetate soluble fractions showed 37.53% and 24.93% inhibition of emesis respectively at dose 400 mg/kg-body weight. In antimicrobial activity assay, the n-hexane soluble fraction (400 µg/disc) showed salient activity against the tested organisms. It exerts highest activity against Salmonella typhi (16.9 mm zone of inhibition); besides, crude, and ethyl acetate extracts showed resistance to Bacillus cereus and Bacillus subtilis, and Vibrio cholera respectively. All the extracts were tested for lysis of the erythrocytes. At the concentration of 1mg/ml, ethanol extract, and n-hexane and ethyl acetate soluble fractions significantly inhibited hypotonic solution induced lysis of the human red blood cell (HRBC) (27.406 ± 3.57, 46.034 ± 3.251, and 30.72 ± 5.679% respectively); where standard drug acetylsalicylic acid (concentration 0.1 mg/ml) showed 77.276 ± 0.321% inhibition. In case of heat induced HRBC hemolysis, the plant extracts also showed significant activity (34.21 ± 4.72, 21.81 ± 3.08, and 27.62 ± 8.79% inhibition respectively). In the brine shrimp lethality bioassay, the n-hexane fraction showed potent (LC50 value 48.978 µg/ml) activity, whereas ethyl acetate fraction showed mild (LC50 value 216.77 µg/ml) cytotoxic activity. CONCLUSIONS: Our results showed that the n-hexane extract has better effects than the other in all trials. In the context, it can be said that the leaves of S. paniculata possess remarkable pharmacological effects, and justify its folkloric use as antimicrobial, antipyretic, anti-inflammatory, and antiemetic agent. Therefore, further research may be suggested to find possible mode of action of the plant part.
Descritores: Asteraceae/química
Citotoxinas/farmacologia
Membrana Eritrocítica/efeitos dos fármacos
Antipiréticos/farmacologia
Antibacterianos/farmacologia
Antieméticos/farmacologia
-Artemia/efeitos dos fármacos
Salmonella typhi/efeitos dos fármacos
Staphylococcus aureus/efeitos dos fármacos
Bacillus cereus/efeitos dos fármacos
Bacillus subtilis/efeitos dos fármacos
Vibrio cholerae/efeitos dos fármacos
Bioensaio/mortalidade
Extratos Vegetais/farmacologia
Testes de Sensibilidade Microbiana
Galinhas
Folhas de Planta/química
Asteraceae/classificação
Etanol
Membrana Eritrocítica/fisiologia
Escherichia coli/efeitos dos fármacos
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Temperatura Alta
Hexanos
Medicina Tradicional
Acetatos
Limites: Humanos
Animais
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950753
Autor: Zeb, Anwar; Sadiq, Abdul; Ullah, Farhat; Ahmad, Sajjad; Ayaz, Muhammad.
Título: Phytochemical and toxicological investigations of crude methanolic extracts, subsequent fractions and crude saponins of Isodon rugosus
Fonte: Biol. Res;47:1-6, 2014. tab.
Idioma: en.
Resumo: BACKGROUND: Isodon rugosus is used traditionally in the management of hypertension, rheumatism, tooth-ache and pyrexia. Present study was arranged to investigate I. rugosus for phytoconstituents, phytotoxic and cytotoxic activities to explore its toxicological, pharmacological potentials and to rationalize its ethnomedicinal uses. Briefly, qualitative phytochemical analysis of the plant extracts were carried out for the existence of alkaloids, flavonoids, saponins, oils, glycosides, anthraquinones, terpenoids, sterols and tannins. Plant crude methanolic extract (Ir.Cr), its subsequent fractions; n-hexane (Ir.Hex), chloroform (Ir.Chf), ethyl acetate (Ir.EtAc), aqueous (Ir.Aq) and saponins (Ir.Sp) in different concentrations were tested for phytotoxic and cytotoxic activities using radish seeds and brine shrimps (Artemia salina) respectively. The phytotoxic activity was determined by percent root length inhibition (RLI) and percent seeds germination inhibition (SGI) while the cytotoxicity was obtained with percent lethality of the brine shrimps. RESULTS: Ir.Cr was tested positive for the presence of alkaloids, glycosides, flavonoids, oils, terpenoids, saponins, tannins and anthraquinones. Among different fractions Ir.Sp, Ir.Chf, Ir.EtAc, and Ir.Cr were most effective causing 93.55, 89.32, 81.32 and 58.68% inhibition of seeds in phytotoxicity assay, with IC50 values of 0.1, 0.1, 0.1 and 52 µg/ml respectively. Similarly, among all the tested samples, Ir.Sp exhibited the highest phytotoxic effect causing 91.33% root length inhibition with IC50 of 0.1 µg/ml. Ir.Sp and Ir.Chf were most effective against brine shrimps showing 92.23 and 76.67% lethality with LC50 values of 10 and 12 µg/ml respectively. CONCLUSIONS: It may be inferred from the current investigations that I. rugosus contains different secondary metabolites and is a potential source for the isolation of natural anticancer and herbicidal drug molecules. Different fractions exhibited phytotoxic and cytotoxic activities, thus providing pharmacological basis for ethnomedicinal uses of this plant.
Descritores: Saponinas/análise
Extratos Vegetais/farmacologia
Isodon/química
Fitoterapia
-Artemia/efeitos dos fármacos
Taninos/análise
Terpenos/análise
Flavonoides/análise
Óleos Vegetais/análise
Clorofórmio
Antraquinonas/análise
Raízes de Plantas/crescimento & desenvolvimento
Citotoxinas
Componentes Aéreos da Planta/química
Metanol
Alcaloides/análise
Glicosídeos/análise
Hexanos
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950761
Autor: Shah, Syed Muhammad Mukarram; Sadiq, Abdul; Shah, Syed Muhammad Hassan; Khan, Shahzeb.
Título: Extraction of saponins and toxicological profile of Teucrium stocksianum boiss extracts collected from District Swat, Pakistan
Fonte: Biol. Res;47:1-5, 2014. tab.
Idioma: en.
Resumo: BACKGROUND: The current era is facing challenges in the management of neoplasia and weeds control. The currently available anti-cancer and herbicidal drugs are associated with some serious side effects. Therefore numerous researchers are trying to discover and develop plant based alternative particularly for the rational management of cancer and weed control. Teucrium stocksianum possess antioxidant and analgesic activities. The current study was designed to evaluate crude saponins (CS), methanolic extract and sub-fractions of T. stocksianum for cytotoxic and phytotoxic potentials. CS, methanolic extract and sub-fractions were extracted from powdered plant material using different solvents. Cytotoxic potential of the extracts at a dose of 10, 100 and 1000 µg/ml were evaluated against Brine shrimp's nauplii. Phytotoxic assay also performed at the same concentration against Lemna minor. Etoposide and Paraquat were used as positive controls in cytotoxic and phytotoxic assays respectively. RESULTS: The percent yield of crude saponins was (5%). CS demonstrated tremendous brine shrimp lethality showing < 10 µg/ml LC50. The n-hexane (HF) and chloroform fractions (CF) demonstrated excellent cytotoxicity with 80 and 55 µg/ml LC50 respectively. Whereas the methanolic extract (TSME), ethyl acetate (EAF) and aqueous fractions (AF) revealed moderate cytotoxicity showing 620, 860 and 1000 µg/ml LC50 values respectively. In phytotoxic assay profound inhibition was displayed by HF (96.67%) and TSME (95.56%, 30 µg/ml LC50) against the growth of Lemna minor at 1000 µg/ml respectively. Both CF and EAF demonstrated profound phytoxicity (93.33%) respectively at highest concentration (1000 µg/ml), while AF and CS demonstrated weak phytotoxicity with 1350 and 710 µg/ml LC50 values respectively. CONCLUSION: Cytotoxicity and phytotoxicity assays indicated that the crude saponins, n-hexane and chloroform fractions of T. stocksianum could play a vital role in the treatment of neoplasia and as potential natural herbicides. Therefore these sub-fractions are recommended for further investigation with the aim to isolate novel anti-cancer and herbicidal compounds.
Descritores: Saponinas/análise
Extratos Vegetais/química
Citotoxinas/farmacologia
Teucrium/química
-Paquistão
Artemia/efeitos dos fármacos
Saponinas/toxicidade
Extratos Vegetais/toxicidade
Araceae/classificação
Araceae/efeitos dos fármacos
Citotoxinas/análise
Teucrium/classificação
Teucrium/toxicidade
Metanol
Hexanos
Dose Letal Mediana
Medicina Tradicional/métodos
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950783
Autor: NK, Asha Tukappa; Londonkar, Ramesh L; Nayaka, Hanumantappa B; CB, Sanjeev Kumar.
Título: Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L
Fonte: Biol. Res;48:1-9, 2015. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: To evaluate the hepatoprotective potential and invitro cytotoxicity studies of whole plant methanol extract of Rumex vesicarius L. Methanol extract at a dose of 100 mg/kg bw and 200 mg/kg bw were assessed for its hepatoprotective potential against CCl4-induced hepatotoxicity by monitoring activity levels of SGOT (Serum glutamic oxaloacetic transaminase), SGPT (Serum glutamic pyruvic transaminase), ALP (Alkaline phosphatase), TP (Total protein), TB (Total bilirubin) and SOD (Superoxide dismutase), CAT (Catalase), MDA (Malondialdehyde). The cytotoxicity of the same extract on HepG2 cell lines were also assessed using MTT assay method at the concentration of 62.5, 125, 250, 500 µg/ml. RESULTS: Pretreatment of animals with whole plant methanol extracts of Rumex vesicarius L. significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver. The biochemical parameters in serum also improved in treated groups compared to the control and standard (silymarin) groups. Histopathological investigation further corroborated these biochemical observations. The cytotoxicity results indicated that the plant extract which were inhibitory to the proliferation of HepG2 cell line with IC50 value of 563.33 ± 0.8 Mg/ml were not cytotoxic and appears to be safe. CONCLUSIONS: Rumex vesicarius L. whole plant methanol extract exhibit hepatoprotective activity. However the cytotoxicity in HepG2 is inexplicable and warrants further study.
Descritores: Extratos Vegetais/farmacologia
Citotoxinas/farmacologia
Rumex/química
Proliferação de Células/efeitos dos fármacos
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico
Fitoterapia/métodos
-Aspartato Aminotransferases/metabolismo
Silimarina/farmacologia
Superóxido Dismutase/metabolismo
Sais de Tetrazólio
Bilirrubina/metabolismo
Tetracloreto de Carbono
Catalase/metabolismo
Anticarcinógenos/farmacologia
Ratos Wistar
Alanina Transaminase/metabolismo
Metanol
Ingestão de Líquidos/efeitos dos fármacos
Ingestão de Alimentos/efeitos dos fármacos
Fosfatase Alcalina/metabolismo
Células Hep G2
Doença Hepática Induzida por Substâncias e Drogas/metabolismo
Doença Hepática Induzida por Substâncias e Drogas/patologia
Formazans
Fígado/efeitos dos fármacos
Fígado/metabolismo
Malondialdeído/metabolismo
Antioxidantes/metabolismo
Antioxidantes/farmacologia
Limites: Humanos
Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-875783
Autor: Rodrigues, Patrícia Siqueira de Melo; Bertolin, Aparecido Osdimir; Fucase, Tamara Mieco; Galluzzi, Fernanda Mouro; Silva, Evellin Caroline; Marumo, Maria Helena Bellini.
Título: Avaliação da atividade citotóxica dos extratos etanólicos da casca e das folhas da Terminalia fagifolia Mart. sobre células normais e tumorais / Assessment of the cytotoxic activity of ethanol extracts from the bark and leaves of terminalia fagifolia mart. on normal and tumor cells
Fonte: J. Health Biol. Sci. (Online);5(1):16-23, jan.-mar./2017. graf.
Idioma: pt.
Resumo: Introdução: A procura por novas alternativas terapêuticas, como as que utilizam as plantas medicinais, tem despertado grande interesse da comunidade científica na busca por tratamentos mais eficientes para as doenças, incluindo o câncer. Terminalia fagifolia Mart. é uma planta medicinal encontrada no Cerrado brasileiro, usada popularmente no tratamento de aftas e tumores. Objetivos: Avaliar a atividade citotóxica dos extratos etanólicos da casca e das folhas da Terminalia fagifolia em linhagens celulares NIH 3T3 e L929 e tumorais PC3 e B16F10. Métodos: Foi realizada a metodologia de determinação da viabilidade celular em ensaio com monocamada de células utilizando o ensaio MTS. As linhagens NIH 3T3, L929, PC3 e B16F10 foram expostas por 24 horas a diferentes concentrações dos extratos etanólicos da casca e folhas da Terminalia fagifolia. Resultados: Os resultados adquiridos mostraram que os extratos apresentaram viabilidade celular, sendo considerada de moderada a alta, para as células normais NIH 3T3 e L929 e citotoxicidade severa para as células tumorais PC3 e B16F10. Dessa forma, torna-se necessária a continuidade dos estudos com essa planta, pois os extratos da casca e das folhas apresentaram atividades antitumorais muito promissoras. Conclusões: Os extratos da casca e das folhas demonstraram viabilidade celular ≥ 50% nas linhagens celulares normais NIH 3T3 e L929 e demonstraram atividade citotóxica para as linhagens tumorais PC3 e B16F10, apresentando redução da viabilidade celular em torno de 60% e 70%, respectivamente. (AU)

Introduction: The search for new therapeutic alternatives, as the ones that use medicinal plants, has awaken a huge interest from the scientific community in seeking through more efficient treatments for diseases, including cancer. Terminalia fagifolia Mart. is a medicinal plant found in Brazilian "Cerrado", popularly used in aphthas and tumor treatment. Objectives: To evaluate the cytotoxic activity of ethanolic extracts of the bark and leaves of the Terminalia fagifolia in cell lines NIH 3T3 and L929 and tumor cells PC3 and B16F10. Methods: The determination methodology in cellular viability was held in an assay with cells monolayer's using the MTS assay. The NIH 3T3, L929, PC3 and B16F10 lines was exposed for 24 hours in differents ethanolic extracts concentrations. Results: The acquired results showed that the extracts had cellular variability is considered moderate to high for the normal cells NIH 3T3 and L929 and severe cytotoxicity to tumor cells PC3 and B16F10. This way, it is necessary to continue studying this plant, since both the bark and leaves extracts have great antitumor activity. Conclusion: The bark and leaves extracts showed cellular variability ≥ 50 % in normal cell lines NIH 3T3 and L929 and demonstrated cytotoxic activity for tumor cells PC3 and B16F10, presenting reduction of cell variability around 60% and 80%, respectively. (AU)
Descritores: Terminalia
-Sobrevivência Celular
Citotoxinas
Responsável: BR1780.2



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