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Visentainer, Jeane Eliete Laguila
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Id: biblio-898948
Autor: Capriolli, Tiago Verri; Visentainer, Jeane Eliete Laguila; Sell, Ana Maria.
Título: Lack of association between Kidd blood group system and chronic kidney disease
Fonte: Rev. bras. hematol. hemoter;39(4):301-305, Oct.-Dec. 2017. tab.
Idioma: en.
Resumo: Abstract Background: The Kidd blood group system has three antigens, Jka, Jkb and Jk3, found on red blood cells and on endothelial cells of the inner lining of blood vessels in the renal medulla. These are known as urea transporter B (UT-B). Researchers have found that individuals carrying the Jk(a − b−) or Jk-null (UT-B null) phenotypes have a lower urine-concentrating capability and risk of severe renal impairment. This study evaluated the distribution of the Kidd phenotypes in patients with chronic kidney disease and a possible association of Kidd antigens with the development of renal disease. Methods: Jka and Jkb antigens were phenotyped using the gel column agglutination test (ID-cards Bio-RAD) in 197 patients with chronic kidney disease and 444 blood donors, as the control group. The phenotype and antigen frequencies between patients and controls were evaluated using the Chi-square method with Yates correction and logistic regression after adjustments for gender and age. Results: No differences were observed between the Kidd phenotypes frequency distribution between patients with chronic kidney disease and blood donors [Jk(a − b+) = 22.3% and 27.2%; Jk(a + b−) = 30.5% and 24.3%; Jk(a + b+) = 47.25% and 48.4%, respectively]. Conclusion: The distribution of Kidd phenotypes found in the studied population is expected for Caucasians; Jka and Jkb antigens and phenotypes were not found to be related to susceptibility for chronic kidney disease.
Descritores: Nitrogênio da Ureia Sanguínea
Sorogrupo
Sistema do Grupo Sanguíneo Kidd
Falência Renal Crônica
Limites: Seres Humanos
Masculino
Feminino
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: biblio-839306
Autor: Ma, ZG; Xia, HQ; Cui, SL; Yu, J.
Título: Attenuation of renal ischemic reperfusion injury by salvianolic acid B via suppressing oxidative stress and inflammation through PI3K/Akt signaling pathway
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(6):e5954, 2017. tab, graf.
Idioma: en.
Projeto: Laiwu Steel Group Hospital.
Resumo: Salvianolic acid B (SAB) is one the major phytocomponents of Radix Salvia miltiorrhiza and exhibit numerous health promoting properties. The objective of the current study was to examine whether SAB exerts a renoprotective effect by attenuating oxidative stress and inflammatory response through activating phosphatidylinositol 3-kinase/serine-threonine kinase B (PI3K/Akt) signaling pathway in a renal ischemic reperfusion rat model. Forty Sprague-Dawley male rats (250–300 g) were obtained and split into four groups with ten rats in each group. The right kidney of all rats was removed (nephrectomy). The rats of the Control group received only saline (occlusion) and served as a sham control group, whereas rats subjected to ischemic reperfusion (IR) insult by clamping the left renal artery served as a postitive control group. The other 2 groups of rats were pretreated with SAB (20 and 40 mg·kg-1·day-1) for 7 days prior IR induction and served as treatment groups (SAB 20+IR; SAB 40+IR). Renal markers creatinine (Cr) and blood urea nitrogen (BUN) were significantly lower in the groups that received SAB. Pretreatment with SAB appears to attenuate oxidative stress by suppressing the production of lipid peroxidation products like malondialdehyde as well as elevating antioxidant activity. The concentration of inflammatory markers and neutrophil infiltration (myeloperoxidase) were significantly decreased. Meanwhile, PI3K protein expression and pAkt/Akt ratio were significantly upregulated upon supplementation with SAB, indicating its renoprotective activity. Taken together, these results indicate that SAB can therapeutically alleviate oxidative stress and inflammatory process via modulating PI3K/Akt signaling pathway and probably ameliorate renal function and thus act as a renoprotective agent.
Descritores: Benzofuranos/farmacologia
Medicamentos de Ervas Chinesas/farmacologia
Estresse Oxidativo/efeitos dos fármacos
Fosfatidilinositol 3-Quinases/metabolismo
Substâncias Protetoras/farmacologia
Proteínas Proto-Oncogênicas c-akt/metabolismo
Traumatismo por Reperfusão/tratamento farmacológico
-Nitrogênio da Ureia Sanguínea
Creatinina/metabolismo
Inflamação/metabolismo
Rim/patologia
Peroxidação de Lipídeos/efeitos dos fármacos
Peroxidase/efeitos dos fármacos
Ratos Sprague-Dawley
Traumatismo por Reperfusão/metabolismo
Transdução de Sinais
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Amaral, Carlos Faria Santos
Silva, Rose Mary Ferreira Lisboa da
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Id: lil-772142
Autor: Melo, Juliana Meneghin de; Magalhães, Juliana Oliveira; Amaral, Carlos Faria Santos; Farah, Kátia de Paula; Silva, Rose Mary Ferreira Lisboa da.
Título: Avaliação da ingestão proteica por meio do registro alimentar em pacientes com doença renal crônica / Evaluation of protein intake by food record in patients with chronic kidney disease
Fonte: RBM rev. bras. med;72(8), ago. 2015.
Idioma: pt.
Resumo: Introdução: A principal manipulação dietética utilizada na tentativa de reduzir a progressão da doença renal crônica (DRC) é o controle da ingestão proteica. Objetivo: analisar a utilização do registro alimentar (RA) para avaliação da ingestão proteica de pacientes com DRC em tratamento conservador, considerando o Equivalente Proteico do Aparecimento de Nitrogênio Ureico (PNA) como método de referência. Metodologia: avaliaram-se os prontuários de pacientes adultos em acompanhamento nutricional no Serviço de Nefrologia do Hospital das Clínicas da Universidade Federal de Minas Gerais, no período de janeiro de 2009 a dezembro de 2011. Foram coletados dados clínicos, epidemiológicos, bioquímicos e RA no prontuário e na ficha de anamnese do serviço. A ingestão proteica estimada por meio do RA foi comparada aos valores de PNA em dois momentos distintos, ambos referentes ao mesmo dia de avaliação. Resultados: foram incluídos no estudo 45 pacientes, sendo 23 (51,1%) do sexo masculino. A idade foi superior a 60 anos em 25 pacientes (55,6%) e a escolaridade média foi de 5,62 ± 3,73 anos de estudo. Não houve correlação entre a avaliação da ingestão proteica pelo RA e o PNA nos dois momentos de avaliação (teste de correlação de Pearson; r=0,06 e p=0,69 e r=0,15 e p=0,32, respectivamente). A ingestão calórica média nos dois momentos de avaliação por meio do RA foi abaixo da recomendação. Conclusão: na amostra estudada não houve correlação do RA com o método de referência PNA para avaliação da ingestão proteica em pacientes com DRC em tratamento conservador.
Descritores: Nitrogênio da Ureia Sanguínea
Alimentos para Praticantes de Atividade Física
Proteínas
Insuficiência Renal Crônica
Limites: Seres Humanos
Masculino
Feminino
Adulto Jovem
Meia-Idade
Responsável: BR12.1 - Biblioteca Setorial da Ciências da Saúde


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Id: lil-771854
Autor: Liu, Guiyong; Song, Hongfei; Qiu, Lili; He, Anren; Tong, Fangfang; Wan, Qifu; Wang, Xin; Xia, Yunfang; Huang, Lequn.
Título: Dexmedetomidine preconditioning inhibits the long term inflammation induced by renal ischemia/reperfusion injury in rats
Fonte: Acta cir. bras;31(1):8-14, Jan. 2016. graf.
Idioma: en.
Resumo: PURPOSE: To investigate the protective effects of dexmedetomidine (Dex) against renal ischemia/reperfusion injury (IRI). METHODS: Sprague-Dawley rats were randomly divided to sham group, IRI group and Dex group. The SD rats were subjected to 45 min of ischemia followed by eight weeks of reperfusion. Prior to ischemia, rats were either treated with Dex or not. Blood samples were collected for the detection of blood urea nitrogen (BUN) and creatinine (Cr) levels. Immunohistochemistry was performed for CD3 T-cell infiltrates. Real-time PCR and western blot were detected for the expression of TNF-α, IL-1β, ICAM-1, HMGB1 and TLR4. RESULTS: Compared with sham group, renal IRI significantly increased the serum levels of BUN and Cr. The H&E staining indicated that renal IRI resulted in obvious renal injury and immunohistochemistry found that there were more CD3 T-cell infiltrates in IRI group. Also, renal IRI upregulated the expression of TNF-α, IL-1β, ICAM-1, HMGB1 and TLR4. However, all these changes were alleviated by the treatment with Dex. CONCLUSIONS: Dexmedetomidine has beneficial effects on long term inflammation induced by renal ischemia/reperfusion injury. Its mechanisms may be achieved through inhibiting the HMGB1/TLR4 pathway to exert protective effects.
Descritores: Lesão Renal Aguda/patologia
/farmacologia
ADRENERGIC ALPHA-TEMEFOS RECEPTOR AGONISTS/farmacologia
Dexmedetomidina/farmacologia
Rim/irrigação sanguínea
Traumatismo por Reperfusão/complicações
-Actinas/análise
Lesão Renal Aguda/etiologia
Lesão Renal Aguda/metabolismo
Nitrogênio da Ureia Sanguínea
Western Blotting
Creatinina/sangue
Proteína HMGB1/análise
Imuno-Histoquímica
Inflamação/etiologia
Inflamação/metabolismo
Molécula 1 de Adesão Intercelular/análise
Interleucina-1beta/análise
Rim/química
Distribuição Aleatória
Ratos Sprague-Dawley
Reação em Cadeia da Polimerase em Tempo Real
RNA
Traumatismo por Reperfusão/patologia
/análise
TOLL-LIKE RECEPTOR ABBREVIATIONS AS TOPIC/análise
Fator de Necrose Tumoral alfa/análise
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: lil-761499
Autor: Wang, Zhi-shun; Liu, Xiu-heng; Wang, Min; Jiang, Guan-jun; Qiu, Tao; Chen, Zhi-yuan; Wang, Lei.
Título: Metformin attenuated the inflammation after renal ischemia/reperfusion and suppressed apoptosis of renal tubular epithelial cell in rats
Fonte: Acta cir. bras;30(9):617-623, Sep. 2015. tab, ilus.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Natural Science Foundation of Hubei Province.
Resumo: PURPOSE:To investigate the effect of metformin on renal tubular epithelial cell apoptosis and inflammation after kidney ischemia/ reperfusion in rats.METHODS:Eighteen SD rats were randomly divided into three groups: Sham (S), Ischemia/reperfusion (I/R), and Metformin (E). Before establishing the I/R model, group E was administered metformin for three days, while groups S and I/R were administered equal volumes of saline. After three days, a right nephrectomy was performed on all groups, after which the left kidneys of groups E and I/R rats were subjected to 45 min renal ischemia. Renal function, histology, and cell apoptosis were assessed. AMPK, pAMPK, COX-2, and Caspase 3 were also detected.RESULTS:Compared to I/R group, Caspase 3 and COX-2 levels were decreased in group E. COX-2, Caspase3 and pAMPK levels were higher in groups E and I/R than in group S. The pAMPK level of group E was higher than that of I/R group, while COX-2 and caspase 3 were lower in group E than they were in the other groups. There was no significant difference between E and I/R groups in AMPK levels.CONCLUSION:Metformin preconditioning attenuated the inflammation caused by ischemia/reperfusion and inhibited the apoptosis of renal tubular epithelial cells.
Descritores: Apoptose/efeitos dos fármacos
Células Epiteliais/efeitos dos fármacos
Precondicionamento Isquêmico/métodos
Rim/irrigação sanguínea
Rim/efeitos dos fármacos
Metformina/farmacologia
Traumatismo por Reperfusão/prevenção & controle
-Proteínas Quinases Ativadas por AMP/análise
Nitrogênio da Ureia Sanguínea
Western Blotting
/análise
CASPASE ABATTOIRS/análise
Creatinina/sangue
/análise
CYCLOOXYGENASE TEMEFOS/análise
Imuno-Histoquímica
Rim/patologia
Distribuição Aleatória
Ratos Sprague-Dawley
Reprodutibilidade dos Testes
Fatores de Tempo
Limites: Animais
Masculino
Tipo de Publ: Estudos de Avaliação
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-757986
Autor: Mousavi, Ghafour.
Título: Study on the effect of black cumin (Nigella sativa Linn.) on experimental renal ischemia-reperfusion injury in rats
Fonte: Acta cir. bras;30(8):542-550, Aug. 2015. tab, ilus.
Idioma: en.
Resumo: PURPOSE: To evaluate the effect of Black cumin (Nigella sativa Linn.) pre-treatment on renal ischemia/reperfusion (I/R) induced injury in the rats.METHODS: A total of 40 male Wistar rats were randomly allocated into five equal groups including Sham, I/R model and three I/R+ Black cumin (0.5, 1 and 2%)-treated groups. I/R groups' kidneys were subjected to 60 min of global ischemia at 37°C followed by 24 h of reperfusion. At the end of reperfusion period, the rats were euthanized. Superoxide dismutase, catalase and glutathione peroxidase activities as well as reduced glutathione and renal malondialdehyde contents were determined in renal tissues. Kidney function tests and histopathological examination were also performed.RESULTS: High serum creatinine, blood urea nitrogen and uric acid as well as malondialhehyde (MDA) levels, and low antioxidant enzyme activities were observed in I/R rats compared to the sham rats. Pre-treatment with Black cumin for three weeks prior to IR operation improved renal function and reduced I/R induced renal inflammation and oxidative injury. These biochemical observations were supported by histopathological test of kidney sections.CONCLUSION:Black cumin significantly prevented renal ischemia/reperfusion induced functional and histological injuries.
Descritores: Rim/irrigação sanguínea
Nigella sativa/química
Preparações de Plantas/uso terapêutico
Traumatismo por Reperfusão/prevenção & controle
-Nitrogênio da Ureia Sanguínea
Creatinina/sangue
Testes de Função Renal
Rim/efeitos dos fármacos
Malondialdeído/análise
Estresse Oxidativo
Peroxidases/sangue
Preparações de Plantas/farmacologia
Distribuição Aleatória
Ratos Wistar
Reprodutibilidade dos Testes
Traumatismo por Reperfusão/sangue
Sementes/química
Fatores de Tempo
Resultado do Tratamento
Ácido Úrico/sangue
Limites: Animais
Masculino
Tipo de Publ: Estudos de Avaliação
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-735709
Autor: Xing, Bianzhi; Chen, Hui; Wang, Lei; Weng, Xiaodong; Chen, Zhiyuan; Li, Xiuheng.
Título: Ozone oxidative preconditioning protects the rat kidney from reperfusion injury via modulation of the TLR4-NF-κB pathway
Fonte: Acta cir. bras;30(1):60-66, 01/2015. graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . National Natural Science Foundation of China.
Resumo: PURPOSE: To investigate the protective effects of ozone oxidative preconditioning (OzoneOP) were associated with the modulation of TLR4-NF-κB pathway. METHODS: Thirty six rats were subjected to 45 min of renal ischemia, with or without treatment with OzoneOP (1 mg/kg). Blood samples were collected for the detection of blood urea nitrogen and creatinine levels. Histologic examinations were evaluated and immunohistochemistry was also performed for localization of TLR4 and NF-κB. The expression of TNF-α, IL-1β, IL-6, ICAM-1 and MCP-1 were studied by Real-time PCR. Western blot was performed to detect the expression of TLR4 and NF-κB. RESULTS: The results indicated that blood urea nitrogen and creatinine levels increased significantly in I/R group. Rats treated with OzoneOP showed obviously less renal damage. Immunohistochemistry showed that TLR4 were ameliorated by OzoneOP. Realtime PCR showed that OzoneOP could significantly inhibit the increased mRNA levels of TNF-α, IL-1β, IL-6, ICAM-1 and MCP-1 induced by I/R. Western blot indicated that the expression of TLR4 and NF-κB were upregulated in I/R group, but OzoneOP could inhibit this increase. CONCLUSION: These findings indicated that OzoneOP had potent anti-inflammatory properties by the modulation of the TLR4-NF-κB pathway in renal ischemia/reperfusion injury. .
Descritores: Precondicionamento Isquêmico/métodos
Rim/irrigação sanguínea
NF-kappa B/análise
Ozônio/farmacologia
Traumatismo por Reperfusão/prevenção & controle
/análise
TOLL-LIKE RECEPTOR ABBREVIATIONS AS TOPIC/análise
-Nitrogênio da Ureia Sanguínea
Western Blotting
Creatinina/sangue
Citocinas/análise
Imuno-Histoquímica
Molécula 1 de Adesão Intercelular/análise
Rim/metabolismo
NF-kappa B/metabolismo
Ratos Wistar
Reação em Cadeia da Polimerase em Tempo Real
Reprodutibilidade dos Testes
Fatores de Tempo
/metabolismo
TOLL-LIKE RECEPTOR ABBREVIATIONS AS TOPIC/metabolismo
Limites: Animais
Masculino
Tipo de Publ: Estudos de Avaliação
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-719189
Autor: Cavalli, Ricardo Cavalheiro; Tambara Filho, Renato; Gomes, Regina de Paula Xavier; Veronez, Djanira Aparecida da Luz; Slongo, Julio; Fraga, Rogério de.
Título: Analysis of the histology of the scar bladder and biochemical parameters of rats with a solitary kidney undergoing immunosuppression with tacrolimus
Fonte: Acta cir. bras;29(8):508-514, 08/2014. tab, graf.
Idioma: en.
Resumo: PURPOSE: To evaluate bladder histology in healing and biochemical analysis of rats with single kidney in ischemia/reperfusion, treated with tacrolimus. METHODS: Fifty rats randomized into five groups. Three rats died in surgery, 47 rats divided in groups: Control (non-operated, n=10), Sham (operated without drugs, n=8), T1 (operated + tacrolimus 1mg/kg, n=10), T2 (operated + tacrolimus 0.1 mg/kg, n=10), T3 (operated + tacrolimus 10mg/kg, n=9). The surgery was: laparotomy, right nephrectomy, left kidney ischemia/reperfusion, cystotomy followed by bladder suture. After that, rats were submited to gavage daily (Control and Sham with saline solution. T1, T2, T3 with tacrolimus in doses already mentioned). On the 14th day, after death induction, cystectomy was performed and bladder was histologicaly analysed. The serum urea, creatinine and tacrolimus were analysed too. RESULTS: There was difference in serum tacrolimus in T3 compared to the other groups (p<0.05). There was higher doses of creatinine in T3 group and higher urea in groups with tacrolimus. There were significant differences among all histologic variables comparing groups with and without tacrolimus (p<0.05). CONCLUSION: Tacrolimus associated with ischemia/reperfusion is nephrotoxic, suppresses inflammation and seems to delay the healing bladder. .
Descritores: Cicatriz/tratamento farmacológico
Imunossupressores/uso terapêutico
Isquemia/complicações
Rim/irrigação sanguínea
Tacrolimo/uso terapêutico
Bexiga Urinária/efeitos dos fármacos
-Nitrogênio da Ureia Sanguínea
Cicatriz/patologia
Creatinina/sangue
Imunossupressores/farmacologia
Modelos Animais
Nefrectomia
Distribuição Aleatória
Ratos Wistar
Traumatismo por Reperfusão/complicações
Tacrolimo/farmacologia
Bexiga Urinária/patologia
Cicatrização/efeitos dos fármacos
Cicatrização/fisiologia
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: lil-716274
Autor: Liu, Y.J.; Yang, Z.Y.; Tan, L.L.; Li, H.; Zhang, Y.Z..
Título: An animal experimental study of porous magnesium scaffold degradation and osteogenesis
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(8):715-720, 08/2014. tab, graf.
Idioma: en.
Projeto: the Hebei Province Government.
Resumo: Our objective was to observe the biodegradable and osteogenic properties of magnesium scaffolding under in vivo conditions. Twelve 6-month-old male New Zealand white rabbits were randomly divided into two groups. The chosen operation site was the femoral condyle on the right side. The experimental group was implanted with porous magnesium scaffolds, while the control group was implanted with hydroxyapatite scaffolds. X-ray and blood tests, which included serum magnesium, alanine aminotransferase (ALT), creatinine (CREA), and blood urea nitrogen (BUN) were performed serially at 1, 2, and 3 weeks, and 1, 2, and 3 months. All rabbits were killed 3 months postoperatively, and the heart, kidney, spleen, and liver were analyzed with hematoxylin and eosin (HE) staining. The bone samples were subjected to microcomputed tomography scanning (micro-CT) and hard tissue biopsy. SPSS 13.0 (USA) was used for data analysis, and values of P<0.05 were considered to be significant. Bubbles appeared in the X-ray of the experimental group after 2 weeks, whereas there was no gas in the control group. There were no statistical differences for the serum magnesium concentrations, ALT, BUN, and CREA between the two groups (P>0.05). All HE-stained slices were normal, which suggested good biocompatibility of the scaffold. Micro-CT showed that magnesium scaffolds degraded mainly from the outside to inside, and new bone was ingrown following the degradation of magnesium scaffolds. The hydroxyapatite scaffold was not degraded and had fewer osteoblasts scattered on its surface. There was a significant difference in the new bone formation and scaffold bioabsorption between the two groups (9.29±1.27 vs 1.40±0.49 and 7.80±0.50 vs 0.00±0.00 mm3, respectively; P<0.05). The magnesium scaffold performed well in degradation and osteogenesis, and is a promising material for orthopedics.
Descritores: Implantes Absorvíveis
Substitutos Ósseos/uso terapêutico
Implantes Experimentais
Magnésio/uso terapêutico
Osteogênese/fisiologia
Tecidos Suporte/química
-Alanina Transaminase/sangue
Nitrogênio da Ureia Sanguínea
Materiais Biocompatíveis/uso terapêutico
Creatinina/sangue
Durapatita/uso terapêutico
Fêmur
Fêmur/cirurgia
Coração/anatomia & histologia
Rim/anatomia & histologia
Fígado/anatomia & histologia
Magnésio/sangue
Porosidade
Baço/anatomia & histologia
Microtomografia por Raio-X
Limites: Animais
Masculino
Coelhos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-715660
Autor: Gonullu, Edip; Ozkardesler, Sevda; Kume, Tuncay; Duru, Leyla Seden; Akan, Mert; Guneli, Mehmet Ensari; Ergur, Bekir Ugur; Meseri, Reci; Dora, Oytun.
Título: Comparison of the effects of dexmedetomidine administered at two different times on renal ischemia/reperfusion injury in rats / Comparação dos efeitos de dexmedetomidina administrada em dois momentos diferentes para lesão de isquemia/reperfusão renal em ratos / Comparación de los efectos de la dexmedetomidina administrada en 2 momentos diferentes para lesión de isquemia-reperfusión renal en ratones
Fonte: Rev. bras. anestesiol;64(3):152-158, May-Jun/2014. tab, graf.
Idioma: en.
Resumo: Background and objectives: We investigated the effect of dexmedetomidine on ischemic renal failure in rats. Methods: In the present study, 26 male adult Wistar albino rats weighting 230-300 g were randomly separated into four groups: sham-operated (n = 5), ischemia reperfusion (IR) (IR group, n = 7), IR/reperfusion treatment with dexmedetomidine (Dex. R group, n = 7) and IR/pre-ischemic treatment with dexmedetomidine (Dex. I group, n = 7). In the first group, sham operation was achieved and renal clamps were not applied. For the IR group, renal ischemia was induced by occlusion of the bilateral renal arteries and veins for 60 min followed by reperfusion for 24 h. For the Dex. R and Dex. I groups, the same surgical procedure as in the IR group was performed, and dexmedetomidine (100 mcg/kg intraperitoneal) was administrated at the 5th min after reperfusion and before ischemia. At the end of reperfusion, blood samples were drawn, the rats were sacrificed, and the left kidney was processed for histopathology. Results: The blood urea nitrogen (BUN) levels in groups Dex. R and Dex. I were significantly lower than in the IR group (p = 0.015, p = 0.043), although urine flow was significantly higher in group Dex. R (p = 0.003). The renal histopathological score in the IR group was significantly higher than in the other groups. There was no significant difference between the Dex. R and Dex. I groups. Conclusions: The results were shown that administration of dexmedetomidine reduced the renal IR injury histomorphologically. Administration of dexmedetomidine in the reperfusion period was considered as more effective due to increase in urinary output and decrease in BUN levels. .

Justificativa e objetivos: Investigar os efeitos de dexmedetomidina sobre a insuficiência renal isquêmica em ratos. Métodos: No presente estudo, 26 ratos machos adultos, albinos Wistar, com peso 230-300 g, foram randomicamente divididos em quatro grupos: pseudo-operado (n = 5), isquemia-reperfusão (grupo IR, n = 7), IR/tratamento de reperfusão com dexmedetomidina (grupo Dex-R, n = 7) e IR/tratamento pré-isquemia com dexmedetomidina (grupo Dex-I, n = 7). No primeiro grupo, uma pseudo-operação foi feita e clampeamentos renais não foram aplicados. No grupo IR, isquemia renal foi induzida por oclusão das artérias e veias renais bilaterais durante 60minutos seguida por reperfusão durante 24horas. Nos grupos Dex-R e Dex-I, o mesmo procedimento cirúrgico destinado ao grupo IR foi feito e dexmedetomidina (100mcg/kg intraperitoneal) foi administrada cinco minutos após a reperfusão e antes da isquemia. No fim da reperfusão, amostras de sangue foram coletadas, os ratos foram sacrificados e os rins esquerdos processado para histologia. Resultados: Os níveis de nitrogênio ureico no sangue (BUN) dos grupos Dex-R e Dex-I estavam significativamente mais baixos do que os do grupo IR (p = 0,015, p = 0,043), embora o fluxo urinário tenha sido significativamente maior no grupo Dex-R (p = 0,003). O escore histopatológico renal do grupo IR foi significativamente maior do que os dos outros grupos. Não houve diferença significativa entre os grupos Dex-R e Dex-I. Conclusões: Os resultados demonstraram que a administração de dexmedetomidina reduziu histomorfologicamente a lesão de IR renal. A administração de dexmedetomidina durante o período de reperfusão foi considerada como mais eficaz por causa do aumento do débito urinário e da diminuição dos níveis de BUN. .

Justificación y objetivos: investigar los efectos de la dexmedetomidina sobre la insuficiencia renal isquémica en ratones. Métodos: en el presente estudio, 26 ratones machos adultos, albinos Wistar, con un peso de 230-300 g fueron divididos aleatoriamente en 4 grupos: seudooperado (n = 5), isquemia-reperfusión (grupo IR, n = 7), IR/tratamiento de reperfusión con dexmedetomidina (grupo Dex-R, n = 7) e IR/tratamiento preisquemia con dexmedetomidina (grupo Dex-I, n = 7). En el primer grupo, se realizó una seudooperación y no se aplicaron pinzamientos renales. En el grupo IR, la isquemia renal fue inducida por oclusión de las arterias y venas renales bilaterales durante 60 min seguida por reperfusión durante 24 h. En los grupos Dex-R y Dex-I, se llevó a cabo el mismo procedimiento quirúrgico destinado al grupo IR, y la dexmedetomidina (100 µg /kg intraperitoneal) fue administrada 5 min después de la reperfusión y antes de la isquemia. Al final de la reperfusión, fueron recogidas muestras de sangre, los ratones fueron sacrificados y el riñón izquierdo procesado para histología. Resultados: los niveles de nitrógeno ureico en la sangre (BUN) de los grupos Dex-R y Dex-I eran significativamente más bajos que los del grupo IR (p = 0,015; p = 0,043), aunque el flujo urinario era significativamente mayor en el grupo Dex-R (p = 0,003). La puntuación histopatológica renal del grupo IR fue significativamente mayor que la de los otros grupos. No hubo diferencia significativa entre los grupos Dex-R y Dex-I. Conclusiones: los resultados demostraron que la administración de dexmedetomidina redujo histomorfológicamente la lesión de IR renal. La administración de dexmedetomidina durante el período de reperfusión fue considerada más eficaz debido al aumento de producción de orina y a la disminución ...
Descritores: Lesão Renal Aguda/prevenção & controle
/farmacologia
ADRENERGIC ALPHA-TEMEFOS RECEPTOR AGONISTS/farmacologia
Dexmedetomidina/farmacologia
Traumatismo por Reperfusão/prevenção & controle
-Lesão Renal Aguda/fisiopatologia
/administração & dosagem
ADRENERGIC ALPHA-TEMEFOS RECEPTOR AGONISTS/administração & dosagem
Nitrogênio da Ureia Sanguínea
Modelos Animais de Doenças
Esquema de Medicação
Dexmedetomidina/administração & dosagem
Ratos Wistar
Traumatismo por Reperfusão/fisiopatologia
Fatores de Tempo
Limites: Animais
Masculino
Ratos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME



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