Base de dados : LILACS
Pesquisa : E01.370.225.500.508 [Categoria DeCS]
Referências encontradas : 25 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 3 ir para página          

  1 / 25 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-887183
Autor: Farag, Azza Gaber Antar; Hammam, Mostafa Ahmed; Habib, Mona SalahEldeen; Elnaidany, Nada Farag; Kamh, Mona Eaid.
Título: Macrophage migration inhibitory factor as an incriminating agent in vitiligo
Fonte: An. bras. dermatol;93(2):191-196, Mar.-Apr. 2018. tab, graf.
Idioma: en.
Resumo: Abstract: Background: Vitiligo is an autoimmune skin disorder in which the loss of melanocytes is mainly attributed to defective autoimmune mechanisms and, lately, there has been more emphasis on autoinflammatory mediators. Among these is the macrophage migration inhibitory factor, which is involved in many autoimmune skin diseases. However, little is known about the contribution of this factor to vitiligo vulgaris. Objective: To determine the hypothesized role of migration inhibitory factor in vitiligo via estimation of serum migration inhibitory factor levels and migration inhibitory factor mRNA concentrations in patients with vitiligo compared with healthy controls. We also aimed to assess whether there is a relationship between the values of serum migration inhibitory factor and/or migration inhibitory factor mRNA with disease duration, clinical type and severity in vitiligo patients. Methods: Evaluation of migration inhibitory factor serum level and migration inhibitory factor mRNA expression by ELISA and real-time PCR, respectively, were performed for 50 patients with different degrees of vitiligo severity and compared to 15 age- and gender-matched healthy volunteers as controls. Results: There was a highly significant increase in serum migration inhibitory factor and migration inhibitory factor mRNA levels in vitiligo cases when compared to controls (p<0.001). There was a significant positive correlation between both serum migration inhibitory factor and migration inhibitory factor mRNA concentrations in vitiligo patients, and each of them with duration and severity of vitiligo. In addition, patients with generalized vitiligo have significantly elevated serum migration inhibitory factor and mRNA levels than control subjects. Study limitations: Small number of investigated subjects. Conclusions: Migration inhibitory factor may have an active role in the development of vitiligo, and it may also be a useful index of disease severity. Consequently, migration inhibitory factor may be a new treatment target for vitiligo patients.
Descritores: Vitiligo/etiologia
Vitiligo/sangue
RNA Mensageiro
Fatores Inibidores da Migração de Macrófagos/análise
Fatores Inibidores da Migração de Macrófagos/fisiologia
-Valores de Referência
Fatores de Tempo
Vitiligo/patologia
Índice de Gravidade de Doença
Estudos de Casos e Controles
Expressão Gênica
Estatísticas não Paramétricas
ELISPOT
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Criança
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Responsável: BR1.1 - BIREME


  2 / 25 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-841779
Autor: Santana, Nadja de Lima; Rêgo, Jamile Leão; Oliveira, Joyce Moura; Almeida, Lucas Frederico de; Braz, Marcos; Machado, Lídia Maria Medeiros; Machado, Paulo Roberto Lima; Castellucci, Léa Cristina.
Título: Polymorphisms in genes TLR1, 2 and 4 are associated with differential cytokine and chemokine serum production in patients with leprosy
Fonte: Mem. Inst. Oswaldo Cruz;112(4):260-268, Apr. 2017. tab, graf.
Idioma: en.
Projeto: INCT-DT.
Resumo: BACKGROUND Leprosy or hansen’s disease is a spectral disease whose clinical forms mostly depends on host’s immune and genetic factors. Different Toll-like receptors (TLR) variants have been described associated with leprosy, but with some lack of replication across different populations. OBJECTIVES To evaluate the role of polymorphisms in genes TLR1, TLR2 and TLR4 and susceptibility to leprosy in a genetic case control study; to verify the association between genotypes of these markers and the immunological profile in the serum of patients with leprosy. METHODS Pre-designed TaqMan® assays were used to genotype markers at TLR1 (rs4833095, rs5743551), TLR2 (rs7656411, rs3804099) and TLR4 (rs1927914, rs1927911). A panel of cytokines and chemokines was accessed by enzime-linked immunosorbent assay (ELISA) test in the serum of a subgroup of patients with and without leprosy reactions. FINDINGS Our results show an association between the T allele of rs3804099 at the TLR2 gene and increased risk for leprosy per se [Odds ratio (OR) = 1.296, p = 0,022]. In addition, evaluating the association between different genotypes of the TLR1, 2 and 4 markers and cytokine/chemokine serological levels, IL-17 appears as an immunological marker regulated by the polymorphism of the three TLR genes evaluated, whereas different TLR1 genotypes were associated with differential production of IL-12p40 and MCP-1(CCL2). Furthermore, other relevant serum markers such as CXCL-10 and IL-6 seemed to be regulated by TLR2 variants and IL-1β was related to TLR4 genotypes. MAIN CONCLUSIONS All together our data points that the tested TLR markers may have a regulatory role in the immunity against Mycobacterium leprae, by driving the host’s production of key cytokines and chemokines involved in the pathogenesis of this disease.
Descritores: Quimiocinas/imunologia
Quimiocinas/sangue
Receptor 1 Toll-Like/genética
Receptor 2 Toll-Like/genética
Receptor 4 Toll-Like/genética
Hanseníase/genética
Hanseníase/imunologia
-Estudos de Casos e Controles
Polimorfismo de Nucleotídeo Único
Alelos
ELISPOT
Genótipo
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Responsável: BR1.1 - BIREME


  3 / 25 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Texto completo
Id: biblio-1023443
Autor: Nunes, André Vinícius.
Título: Influência da Hidroquinona nas respostas imune humoral e celular induzida por vacina viral com proteína recombinante (Dengue) / The Influence of Hydroquinone in the humoral and cellular immune responses elicited by vaccination with Dengue envelope recombinant Protein.
Fonte: São Paulo; s.n; 2019. 186 p. tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Ciências Farmacêuticas para obtenção do grau de Mestre.
Resumo: A fumaça do cigarro apresenta mais de 8700 substâncias identificadas, as quais já foram relacionadas com o desenvolvimento das mais variadas doenças. Dentre elas, uma substância relevante neste contexto de toxicidade do cigarro é a hidroquinona (HQ), gerada após a biotransformação do benzeno inalado. A HQ apresenta atividades relacionadas com a imunossupressão das respostas imune inata e adaptativa, observados mais no contexto in vitro e parcamente no in vivo; contudo, nenhum estudo ainda trouxe a abordagem do efeito da exposição à HQ sobre a resposta induzida por vacinação. Sendo assim, será que a exposição à fumaça do cigarro ou à HQ influenciaria na resposta de células B e geração de anticorpos induzidas por imunizações com vacinas anti-virais? Observamos que, após a exposição diária com 2500 ppm de HQ (equivalente a um maço de cigarro fumado / dia) por 8 semanas e vacinação com proteína recombinante codificadora do domínio III do Envelope do vírus da Dengue sorotipo 2 (EDIII) mais o adjuvante Alum, houve uma "tendência" para menores títulos de IgG total e IgG1 específicos à EDIII em camundongos C57BL/6. Análises histológicas revelaram um menor número de folículos e redução significativa de suas áreas no baço do grupo HQ em comparação com os não expostos. Para entendermos o efeito da HQ sobre a resposta humoral, realizamos uma análise de dados públicos de transcriptoma obtidas de amostras de sangue de humanos. Curiosamente, observamos que a HQ regula positivamente genes relacionados com a ativação de células B, assim como a migração e quimiotaxia de neutrófilos e outros leucócitos. Como é sabido que existe uma população de neutrófilos (N2) com a capacidade de auxiliar as respostas de células B, hipotetizamos que essas células poderiam disparar um mecanismo imunocompensatório que aumenta os títulos de anticorpos no grupo HQ

The cigarette smoke has more than 8700 harmful substances related to the occurrence of the most varied diseases. Among them, a relevant substance is the hydroquinone (HQ), generated upon the biotransformation of inhaled benzene. In vitro and in vivo analyses have demonstrated that HQ can suppress both innate and adaptive immune responses. However, no study has approached the effect of the HQ exposure on the vaccination-induced response. Thus, would the exposure to the cigarette smoke or HQ influence the B-cell and antibody responses elicited by immunizations with antiviral vaccines? We observed a "tendency" to lower titers of IgG total and IgG1 anti-EDIII in mice daily exposed to 2,500 ppm of HQ for 8 weeks and vaccinated. Histological analyses revealed a smaller number of follicles and a significant reduction in their area in the HQ group in comparison to their counterparts. In order to understand the effect of the HQ on the humoral response, we performed an analysis of public transcriptome data derived from human blood samples. We observed that the HQ up-regulates the expression of genes related to B cell activation as well as the migration and chemotaxis of neutrophils and other leukocytes. Considering that N2 neutrophils have the ability to help the B cell response, we have hypothesized that the HQ exposure may trigger an immunocompensatory effect, increasing the humoral response
Descritores: Vacinas/farmacologia
Dengue
Hidroquinonas/análise
-Ensaio de Imunoadsorção Enzimática/métodos
ELISPOT/métodos
Transcriptoma/genética
Produtos do Tabaco/efeitos adversos
Limites: Animais
Masculino
Camundongos
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas
BR40.1; T615.9, N972i. 30100022637-F


  4 / 25 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-841807
Autor: Araujo, Perla F; Almeida, Adriana B; Pimentel, Carlos F; Silva, Adriano R; Sousa, Alessandro; Valente, Sebastião A; Valente, Vera C; Britto, Manuela M; Rosa, Ana C; Alves, Rozeneide M; Hagström, Luciana; Teixeira, Antonio RL.
Título: Sexual transmission of American trypanosomiasis in humans: a new potential pandemic route for Chagas parasites
Fonte: Mem. Inst. Oswaldo Cruz;112(6):437-446, June 2017. tab, graf.
Idioma: en.
Resumo: BACKGROUND The Trypanosoma cruzi infection endemic in Latin America has now spread to several countries across four continents; this endemic involves triatomine vector-free protists. We hypothesised that the sexual transmission of T. cruzi contributes to the ongoing spread of Chagas disease. OBJECTIVES A short-term longitudinal study was conducted to evaluate this hypothesis. METHODS The study population comprised 109 subjects from four families, among whom 21 had been diagnosed with acute Chagas disease by direct parasitological analysis. Blood mononuclear cells and serum samples were obtained from each study subject once per year for three consecutive years. Enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence serological examinations were used to detect specific T. cruzi antibodies. Polymerase chain reaction of T. cruzi DNA revealed 188-nucleotide bands, which hybridised to a specific radiolabelled probe and were confirmed by cloning and sequencing. RESULTS Three independent assessments at different time points revealed T. cruzi nuclear DNA footprints in 76% (83/109) of the study population with active infection. In contrast, the ELISA and indirect immunofluorescence assays detected the T. cruzi antibody in 28.4% (31/109) of the study samples. Moreover, the semen from 82.6% (19/23) of subjects people revealed harboured the 188- bp base pair T. cruzi footprint. Interestingly, the ejaculates of nuclear DNA-positive Chagas patient transmitted the T. cruzi upon peritoneal injection or infusion in the vagina of mice, and amastigotes were detected in the skeletal muscle, myocardium, vas deferens, and uterine tube. MAIN CONCLUSIONS T. cruzi infections can be transmitted from females or males to naïve mates through intercourse, and progeny showed discrepancies between the ratios of nuclear DNA footprints and specific antibody that can be explained by the tolerance attained during early embryo growth. Additional studies are needed to develop drugs to eradicate the infections. Additionally, the importance of a vigorous education, information, and communication program to prevent sexually transmitted Chagas disease in humans cannot be underemphasised.
Descritores: Trypanosoma cruzi/genética
Trypanosoma cruzi/imunologia
Doenças Sexualmente Transmissíveis/epidemiologia
Doença de Chagas/transmissão
Doença de Chagas/epidemiologia
ELISPOT
-Brasil/epidemiologia
Reação em Cadeia da Polimerase
Estudos Longitudinais
Imunofluorescência
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Responsável: BR1.1 - BIREME


  5 / 25 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-790388
Autor: Hong, Marisa Ailin.
Título: Resposta imune celular contra peptídeos crípticos do HIV-1 / Cellular immune response against HIV-1 cryptic peptides.
Fonte: São Paulo; s.n; 2014. [106] p. tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Medicina para obtenção do grau de Doutor.
Resumo: INTRODUÇÃO E OBJETIVOS: Uma fonte secundária e não convencional de peptídeos que se ligam as moléculas MHC de classe I tem sido descrita como responsável por produzir peptídeos crípticos. Esses peptídeos são imunogênicos e portanto, capazes de induzir uma resposta imunológica por células T e assim, contribuir com a resposta total exercida pelas células T CD8+, colaborando na pressão que leva HIV-1 ao processo de mutação, e consequentemente ao escape viral. Alguns pacientes, que correspondem a menos de 5% da população infectada, são capazes de naturalmente controlar a progressão da doença, mantendo a contagem de célula T CD4+ acima de 500 células/uL ou mantendo a carga viral abaixo de 2.000 cópias/mL, por ao menos 12 meses, sem ser submetido a tratamento com antirretrovirais ou esquema HAART. Avaliar a resposta imunológica destes pacientes, controladores da infecção, contra peptídeos crípticos pode nos fornecer informações importantes que colaborem com o desenvolvimento de novas estratégias preventivas. METODOLOGIA: A resposta imunológica contra peptídeos crípticos, estes derivados da transcrição da seqüência consenso e da seqüência inversa do gene do HIV-1, foram avaliados em vários conjuntos (pools), utilizando amostras coletadas de pacientes controladores, tanto avirêmicos, também conhecidos como controladores de elite (carga viral < limite de detecção), bem como virêmicos (carga viral < 2.000 cópias/mL) e, de pacientes progressores. Foi observada que a resposta imunológica contra peptídeos crípticos é mais freqüente, com maior amplitude e magnitude entre os pacientes controladores comparados ao que foi observado entre pacientes progressores. Esta resposta, entretanto, parece inverter ao longo da infecção, como observada utilizando as amostras coletadas em momento tardio da infecção, onde os controladores parecem perder sua capacidade de responder aos peptídeos crípticos, enquanto que os progressores desenvolveram resposta, ressaltando...

BACKGROUND: A second and unconventional source of peptides that bind to MHC class I molecule has been described to produce cryptic peptides, which are immunogenic and are able elicit T cell response, that contributes to total CD8+ T cell immune response and then exert mutation pressure on HIV-1, leading to virus escape. Some rare patients, less than 5% of infected population, are naturally able to control disease progression, either maintaining CD4+ T cells over 500 cells/uL or viral load under 2,000 copies/mL, without being treated with HAART, for at least 12 months. Understanding their immune response to cryptic peptides might be a great value to help on developing better prevention strategies. METHODOLOGY: Immune response to cryptic peptides, derived from sense and antisense transcription of HIV-1, was evaluated in pools using samples from Elite (aviremic) or HIV (viremic, < 2,000 copies/mL) controllers and progressors. Immune response to cryptic peptides are more frequent, with a larger breadth and of greater magnitude in controllers than in progressors, and this response is inversed seen in a later time point, when controllers seems to lose this response, while progressors developed it, showing cryptic peptides immune response to different pools, suggesting that immune response to cryptic peptides might play some role in pressuring the virus mutation escape. CONCLUSIONS AND SIGNIFICANCE: cryptic peptides can elicit immune response and help to explain how some virus selection happens, either by changing expression of crucial HIV-1 proteins or generating defective virus. They can be included in vaccine design for enhancing the magnitude and breadth of T cell immune response and consequently the protection against infection or progression of HIV-1 infection.
Descritores: ELISPOT
Antígenos HIV
Infecções por HIV
HIV-1
Limites: Humanos
Feminino
Adulto
Responsável: BR66.1 - Divisão de Biblioteca e Documentação
BR66.1


  6 / 25 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-769838
Autor: Santana, Vinicius Canato; Almeida, Rafael Ribeiro; Ribeiro, Susan Pereira; Ferreira, Luís Carlos de Souza; Kalil, Jorge; Rosa, Daniela Santoro; Cunha Neto, Edecio.
Título: Co-administration of plasmid-encoded granulocyte-macrophage colony-stimulating factor increases human immunodeficiency virus-1 DNA vaccine-induced polyfunctional CD4+ T-cell responses
Fonte: Mem. Inst. Oswaldo Cruz;110(8):1010-1016, Dec. 2015. graf.
Idioma: en.
Projeto: CNPq; . FAPESP; . FAPESP; . FAPESP.
Resumo: T-cell based vaccines against human immunodeficiency virus (HIV) generate specific responses that may limit both transmission and disease progression by controlling viral load. Broad, polyfunctional, and cytotoxic CD4+T-cell responses have been associated with control of simian immunodeficiency virus/HIV-1 replication, supporting the inclusion of CD4+ T-cell epitopes in vaccine formulations. Plasmid-encoded granulocyte-macrophage colony-stimulating factor (pGM-CSF) co-administration has been shown to induce potent CD4+ T-cell responses and to promote accelerated priming and increased migration of antigen-specific CD4+ T-cells. However, no study has shown whether co-immunisation with pGM-CSF enhances the number of vaccine-induced polyfunctional CD4+ T-cells. Our group has previously developed a DNA vaccine encoding conserved, multiple human leukocyte antigen (HLA)-DR binding HIV-1 subtype B peptides, which elicited broad, polyfunctional and long-lived CD4+ T-cell responses. Here, we show that pGM-CSF co-immunisation improved both magnitude and quality of vaccine-induced T-cell responses, particularly by increasing proliferating CD4+ T-cells that produce simultaneously interferon-γ, tumour necrosis factor-α and interleukin-2. Thus, we believe that the use of pGM-CSF may be helpful for vaccine strategies focused on the activation of anti-HIV CD4+ T-cell immunity.
Descritores: Vacinas contra a AIDS/imunologia
Antígenos Virais/imunologia
/imunologia
CDABBREVIATIONS AS TOPIC-POSITIVE T-LYMPHOCYTES/imunologia
Fator Estimulador de Colônias de Granulócitos e Macrófagos/administração & dosagem
HIV-1
Imunidade Celular/imunologia
Vacinas de DNA/imunologia
-Adjuvantes Imunológicos/administração & dosagem
/efeitos dos fármacos
CDABBREVIATIONS AS TOPIC-POSITIVE T-LYMPHOCYTES/efeitos dos fármacos
Movimento Celular/efeitos dos fármacos
Movimento Celular/imunologia
Sequência Conservada/imunologia
ELISPOT
Citometria de Fluxo
Vetores Genéticos
Fator Estimulador de Colônias de Granulócitos e Macrófagos/imunologia
Infecções por HIV/prevenção & controle
Antígenos HLA-DR/imunologia
Interferon gama/efeitos dos fármacos
Interferon gama/metabolismo
/metabolismo
INTERLEUKIN-TEMEFOS/metabolismo
Ativação Linfocitária/efeitos dos fármacos
Ativação Linfocitária/imunologia
Camundongos Endogâmicos BALB C
Plasmídeos
Ligação Proteica/imunologia
Fator de Necrose Tumoral alfa/efeitos dos fármacos
Fator de Necrose Tumoral alfa/metabolismo
Limites: Animais
Feminino
Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  7 / 25 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: lil-742544
Autor: Contardo, Verónica; Cofré, José; Hernández, Pamela.
Título: Mal de Pott y diagnóstico inmunológico de tuberculosis, a propósito de un caso pediátrico / Pott's disease and immunological diagnosis of tuberculosis, about a pediatric clinical case
Fonte: Rev. chil. infectol;32(1):136-141, feb. 2015. ilus.
Idioma: es.
Resumo: Tuberculosis (TB) remains a major health problem in the world. The clinical forms of TB in children are variable, pulmonary involvement occurs in two thirds of cases. In the remaining third, clinical forms incluye node, meningeal and osteoarticular involvement. Case report: 7 year old boy with a history of an osteolytic lesion of the right ischial branch. Three months later he presented with spondylodiscitis at L2-L3, associated with a large abscess in the right iliac psoas muscle. Pott's disease was suspected, and tuberculin test and T-SPOT®.TB test were performed, with a positive result. Antimicrobial treatment was initiated with isoniazid, rifampicin, pyrazinamide and ethambutol. After 30 days, Mycobacterium tuberculosis was isolated from psoas abscess. We discuss methods of TB diagnosis, with special emphasis on immunological methods: tuberculin test and interferon-gamma release assays. Methods of immunological TB diagnosis are an important contribution to the diagnosis of this disease, allowing early initiation of treatment.

La tuberculosis sigue siendo un importante problema en salud en el mundo. Las formas clínicas de TBC en los niños son muy variadas, presentándose en dos tercios de los casos compromiso pulmonar. En el tercio restante destacan los compromisos ganglionar, meníngeo y osteoarticular. Caso clínico: varón de 7 años que presentó una espondilodiscitis L2-L3, asociada a un absceso en músculo psoas-ilíaco derecho. Por sospecha de mal de Pott se realizó PPD y T-SPOT®.TB que resultaron positivos. Se inició tratamiento antimicrobiano asociado con isoniazida, rifampicina, pirazinamida y etambutol. Después de 30 días, se aisló Mycobacterium tuberculosis del absceso del psoas. Se discute los métodos de diagnóstico de TBC en pediatría, con especial énfasis en los métodos inmunológicos: reacción de tuberculina y test de liberación de interferón-gamma, los que son una importante contribución para el diagnóstico de esta enfermedad, permitiendo el pronto inicio de su tratamiento.
Descritores: Tuberculose da Coluna Vertebral/diagnóstico
-Discite/diagnóstico
ELISPOT
Testes Imunológicos
Vértebras Lombares
Mycobacterium tuberculosis/isolamento & purificação
Abscesso do Psoas/diagnóstico
Teste Tuberculínico
Limites: Criança
Humanos
Masculino
Tipo de Publ: Relatos de Casos
Responsável: CL1.1 - Biblioteca Central


  8 / 25 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-732606
Autor: Silva, Bosco Christiano Maciel da; Grassi, Maria Fernanda Rios; Coutinho, Raimundo; Mascarenhas, Rita Elizabeth Moreira; Olavarria, Viviana Nilla; Coutinho-Borgo, Adriana; Kalil, Jorge; Cunha Neto, Edecio; Fonseca, Simone Gonçalves.
Título: Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test
Fonte: Mem. Inst. Oswaldo Cruz;109(8):999-1004, 12/2014. tab, graf.
Idioma: en.
Resumo: The interferon (IFN)-&#947; response to peptides can be a useful diagnostic marker of Mycobacterium tuberculosis (MTB) latent infection. We identified promiscuous and potentially protective CD4+ T-cell epitopes from the most conserved regions of MTB antigenic proteins by scanning the MTB antigenic proteins GroEL2, phosphate-binding protein 1 precursor and 19 kDa antigen with the TEPITOPE algorithm. Seven peptide sequences predicted to bind to multiple human leukocyte antigen (HLA)-DR molecules were synthesised and tested with IFN-&#947; enzyme-linked immunospot (ELISPOT) assays using peripheral blood mononuclear cells (PBMCs) from 16 Mantoux tuberculin skin test (TST)-positive and 16 TST-negative healthy donors. Eighty-eight percent of TST-positive donors responded to at least one of the peptides, compared to 25% of TST-negative donors. Each individual peptide induced IFN-&#947; production by PBMCs from at least 31% of the TST-positive donors. The magnitude of the response against all peptides was 182 &#177; 230 x 106 IFN-&#947; spot forming cells (SFC) among TST-positive donors and 36 &#177; 62 x 106 SFC among TST-negative donors (p = 0.007). The response to GroEL2 (463-477) was only observed in the TST-positive group. This combination of novel MTB CD4 T-cell epitopes should be tested in a larger cohort of individuals with latent tuberculosis (TB) to evaluate its potential to diagnose latent TB and it may be included in ELISPOT-based IFN-&#947; assays to identify individuals with this condition.
Descritores: /imunologia
CDABBREVIATIONS AS TOPIC-POSITIVE T-LYMPHOCYTES/imunologia
Epitopos/imunologia
Interferon gama/metabolismo
Tuberculose Latente/diagnóstico
Mycobacterium tuberculosis/imunologia
Teste Tuberculínico
-Algoritmos
Antígenos de Bactérias/análise
Brasil
Proteínas de Bactérias/sangue
Biomarcadores/análise
/metabolismo
CDABBREVIATIONS AS TOPIC-POSITIVE T-LYMPHOCYTES/metabolismo
Chaperoninas/sangue
ELISPOT
Mapeamento de Epitopos
Voluntários Saudáveis
Antígenos HLA-DR/imunologia
Tuberculose Latente/imunologia
Leucócitos Mononucleares/imunologia
Leucócitos Mononucleares/metabolismo
Proteínas de Ligação a Fosfato/sangue
Limites: Adulto
Humanos
Pessoa de Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  9 / 25 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-719953
Autor: Ortigosa, Luciena Cegatto Martins.
Título: Avaliação da resposta imune anti-Mycobacterium tuberculosis em pacientes com psoríase moderada a grave submetidos à terapia com inibidor de fator de necrose tumoral, infliximabe / Evaluated of immune responses anti-Mycobacterium tuberculosis in patients with psoriasis moderade to severe undergoing treatment with TNF blocking agent, infliximab.
Fonte: São Paulo; s.n; 2013. 103 p. ilus, tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Medicina para obtenção do grau de Doutor.
Resumo: O tratamento de pacientes apresentando doenças inflamatórias imunomediadas com drogas anti-TNF-alfa aumenta o risco da reativação da tuberculose. Isso sugere que tais drogas possam afetar a imunidade celular destes. No entanto, há dados conflitantes sobre se esse tratamento suprime as respostas para o teste tuberculínico (TT) e os ensaios de liberação de interferon-gama (IGRAs) e poucos dados em pacientes com psoríase. O presente estudo avaliou pacientes com psoríase moderada a grave enfocando os efeitos do tratamento com infliximabe em suas respostas imunológicas celulares. Foram avaliadas as respostas imunes celulares de doze pacientes antes e durante o tratamento com infliximabe. As células mononucleares do sangue periférico (PBMC) foram estimuladas com a fito-hemaglutinina (PHA), o superantígeno enterotoxina B (SEB), um lisado de citomegalovírus (CMV), e antígenos de Mycobacterium tuberculosis, e a ativação de linfócitos foi avaliada por ELISPOT para enumerar células secretoras de IFN-y, por ELISA para detecção da secreção de IFN-y, e através da incorporação de[3H] timidina para medir a proliferação. O tratamento com infliximabe não levou à redução de INF-y e da resposta linfoproliferativa nos pacientes. Pelo contrário, aumentou a liberação desta citocina em culturas de PBMC estimulados com PHA e SEB por 12 h. Este efeito foi mais notado no pico do efeito clínico do agente anti-TNF (7 semanas de tratamento) e menos proeminente no seu nadir (logo antes da infusão da próxima dose). Reatividade imunitária ao CMV também não foi significativamente afetada, notando-se leve aumento pelo agente anti-TNF. É de se notar que secreção de IFN-y e resposta proliferativa a Mtb dos dois pacientes TT positivos foram, também, visivelmente aumentadas na semana 7, declinando quando infliximabe atingiu o seu nadir. Os efeitos deletérios do bloqueio do TNF em pacientes com psoríase grave, submetidos ao tratamento com infliximabe parecem ser atenuados, pelo menos parcialmente...

Treatment of patients with immune-mediated inflammatory diseases with anti-TNF agents increases the risk of tuberculosis reactivation, suggesting that it may affect their cellular immune response. However, there are conflicting data on whether anti-TNF treatment suppresses the responses to tuberculin skin test (TST) and interferon-y release assays and no information regarding psoriasis patients on anti-TNF treatment. The present study evaluated patients with moderate to severe psoriasis focusing on the effects of treatment with infliximab on their cellular immune responses. Cellular immune responses of twelve patients were evaluated before and during infliximab treatment. Peripheral blood mononuclear cells (PBMC) were stimulated with phytohemaglutinin (PHA), the superantigen enterotoxin B (SEB), a cytomegalovirus lysate (CMV), and Mycobacterium tuberculosis antigens, and the lymphocyte activation was evaluated by ELISPOT for enumeration of IFN-y-secreting cells, ELISA for detection of secreted IFN-y, and by [3H]thymidine incorporation for proliferation measurement. Treatment with infliximab does not lead to reduction in the INF-y and lymphoproliferative responses of patients. It rather increased the overnight release of this cytokine in PBMC cultures stimulated with PHA and SEB. This effect was most noted at the peak of the clinical effect of the anti-TNF agent (week 7 of treatment) and less prominent at its nadir (just before infusion of the next dose). Immune reactivity to CMV was also either unaffected or slightly increased by the TNF blocking agent. Of note, the IFN-y and proliferative responses to Mtb from the two TST-responder patients were also remarkably increased at week 7, declining when infliximab reached its nadir. The deleterious consequences of TNF blockade in patients with severe psoriasis undergoing infliximab treatment may be in part attenuated by an enhancing effect on the cell mediated immunity of the patients, possibly due to the...
Descritores: Anticorpos Monoclonais
ELISPOT
Psoríase/terapia
Tuberculose
Fator de Necrose Tumoral alfa
Limites: Humanos
Masculino
Feminino
Adulto
Responsável: BR66.1 - Divisão de Biblioteca e Documentação
BR66.1


  10 / 25 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-689877
Autor: Yuan, Kai; Zhong, Zhao-ming; Zhang, Qiang; Xu, Shu-chai; Chen, Jian-ting.
Título: Evaluation of an enzyme-linked immunospot assay for the immunodiagnosis of atypical spinal tuberculosis (atypical clinical presentation/atypical radiographic presentation) in China
Fonte: Braz. j. infect. dis;17(5):529-537, Sept.-Oct. 2013. ilus, tab.
Idioma: en.
Resumo: BACKGROUND: Atypical spinal tuberculosis (TB) usually presents in a slowly indolent manner with nonspecific clinical presentations making the diagnosis a great challenge for physicians. New technologies for the detection of atypical spinal TB are urgently needed. The aim of this study was to assess the diagnostic value of an enzyme-linked immunospot (ELISPOT) assay in clinically suspected cases of atypical spinal TB in China. METHODS: From March 2011 to September 2012, a total of 65 patients with suspected atypical spinal TB were enrolled. In addition to conventional tests for TB, we used ELISPOT assays to measure the IFN-I response to ESAT-γ and CFP-10 in T-cells in samples of peripheral blood mononuclear cells. Patients with suspected atypical spinal TB were classified by diagnostic category. Data on clinical characteristics of the patients and conventional laboratory results were collected. RESULTS: Out of 65 patients, 4 were excluded from the study. 18 (29.5%) subjects had cultureconfirmed TB, 11 (18.0%) subjects had probable TB, and the remaining 32 (52.5%) subjects did not have TB. Generally, the features of atypical spinal TB include the following aspects: (1) worm-eaten destruction of vertebral endplate; (2) destruction of centricity of the vertebral body or concentric collapse of vertebral body; (3) tuberculous abscess with no identifiable osseous lesion; (4) contiguous or skipped vertebral body destruction. 26 patients with atypical spinal TB had available biopsy or surgical specimens for histopathologic examination and 23 (88.5%) specimens had pathologic features consistent with TB infection. The sensitivities of the PPD skin test and ELISPOT assay for atypical spinal TB were 58.6% and 82.8%, and their specificities were 59.4% and 81.3%, respectively. Malnutrition and age were associated with ELISPOT positivity in atypical spinal TB patients. CONCLUSIONS: The ELISPOT assay is a useful adjunct to current tests for diagnosis of atypical spinal TB.
Descritores: ELISPOT
Mycobacterium tuberculosis/imunologia
Tuberculose da Coluna Vertebral/diagnóstico
-Biópsia
China
Sensibilidade e Especificidade
Tuberculose da Coluna Vertebral/patologia
Limites: Adolescente
Adulto
Idoso
Criança
Pré-Escolar
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Adulto Jovem
Tipo de Publ: Estudo de Avaliação
Estudo Multicêntrico
Responsável: BR1.1 - BIREME



página 1 de 3 ir para página          
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde