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Id: biblio-1133772
Autor: Silva, Aline da; Piccinato, Carla de Azevedo; Sardinha, Luiz Roberto; Aloia, Thiago Pinheiro Arrais; Goldberg, Anna Carla.
Título: Comparison of senescence progression in mesenchymal cells from human umbilical cord walls measured by immunofluorescence and flow cytometry of p16 and p21 / Comparação da progressão da senescência em células mesenquimais de parede de cordão umbilical humano medida por imunofluorescência e citometria de fluxo de p16 e p21
Fonte: Einstein (Säo Paulo);18:eAO5236, 2020. graf.
Idioma: en.
Resumo: ABSTRACT Objective To follow the expansion of mesenchymal stem cells from umbilical cords by two classic senescence markers, p16 (INK4A) and p21 (CDKN1A), using practical, fast, and less expensive methods than the gold standard Western blotting technique, to evaluate its applicability in the laboratory. Methods Mesenchymal stem cells from umbilical cords were isolated from Wharton's jelly and, after quality control, morphological and immunophenotypic characterization by flow cytometry, were expanded in culture until coming close to cell cycle arrest (replicative senescence). Results A comparison was made between young cells, at passage 5, and pre-senescent cells, at passage 10, evaluating the protein expression of the classic cell senescence markers p16 and p21, comparing the results obtained by Western blotting with those obtained by flow cytometry and indirect immunofluorescence. Conclusion Follow-up of cell cultures, through indirect p16 immunofluorescence, allows the identification of mesenchymal stem cells from umbilical cord cultures at risk of reaching replicative senescence.

RESUMO Objetivo Acompanhar a expansão de células-tronco mesenquimais de cordão umbilical por dois marcadores clássicos de senescência, p16 (INK4A) e p21 (CDKN1A), usando métodos práticos, rápidos e com custo menor do que a técnica padrão-ouro de Western blotting, para avaliar sua aplicabilidade em laboratório. Métodos Células-tronco mesenquimais de cordão umbilical foram isoladas da geleia de Wharton e, após controle de qualidade e caracterização morfológica e imunofenotípica por citometria de fluxo, foram expandidas em cultura, até chegarem próximas à parada do ciclo celular (senescência replicativa). Resultados Foi feita a comparação entre células jovens, na passagem 5, e células pré-senescentes, na passagem 10, avaliando a expressão proteica dos marcadores clássicos de senescência celular p16 e p21, comparando os resultados obtidos por Western blotting com os obtidos por citometria de fluxo e imunofluorescência indireta. Conclusão O seguimento de culturas celulares, por meio da imunofluorescência indireta de p16, permite identificar as culturas de células-tronco mesenquimais de cordão umbilical em risco de atingirem a senescência replicativa.
Descritores: Cordão Umbilical/fisiologia
Imunofluorescência/métodos
Senescência Celular
Células-Tronco Mesenquimais/fisiologia
Citometria de Fluxo/métodos
-Biomarcadores/sangue
Células Cultivadas
Western Blotting
Inibidor p16 de Quinase Dependente de Ciclina
Inibidor de Quinase Dependente de Ciclina p21
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1249009
Autor: Lins Filho, Gastão Tenório; Barbosa, Nathalia Lages Sarmento; Abreu, Eulina Maria Vieira de; Costa, Klinger Vagner Teixeira da; Meneses, Kelly Chrystine Barbosa; Silva, Rodrigo Neves; Ferreira, Sonia Maria Soares.
Título: Childhood pemphigus vulgaris is a challenging diagnosis
Fonte: Autops. Case Rep;11:e2021267, 2021. graf.
Idioma: en.
Resumo: Pemphigus Vulgaris (PV) is an uncommon autoimmune and blistering mucocutaneous disease. Childhood Pemphigus Vulgaris (CPV) is a pediatric variant of PV, which affects children below 12 years, being very rare among children under 10 years of age. CPV has similar clinical, histological, and immunological features as seen in PV in adults. The mucocutaneous clinical presentation is the most common in both age groups. Vesicles and erosions arising from the disease usually cause pain. A few CPV cases have been reported in the literature. This study reports a case of an 8-year-old male patient with oral lesions since the age of 3 years, and the diagnosis of pemphigus was achieved only 2 years after the appearance of the initial lesions. CPV remains a rare disease, making the diagnosis of this clinical case a challenge due to its age of onset and clinical features presented by the patient. Therefore, dentists and physicians should know how to differentiate CPV from other bullous autoimmune diseases more common in childhood.
Descritores: Pênfigo/complicações
-Imunofluorescência
Doenças Raras
Limites: Humanos
Masculino
Criança
Tipo de Publ: Relatos de Casos
Responsável: BR26.7 - Serviço de Biblioteca e Documentação Científica


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Id: biblio-990014
Autor: Rodríguez-Rodríguez, Víctor Emanuel; Quiroga-Garza, Alejandro; Rodríguez-Roque, Carlos Saúl; Loera-Arias, María de Jesús; Soto-Domínguez, Adolfo; Guzmán-López, Santos; Vilchez-Cavazos, José Félix; Montes-de-Oca-Luna, Roberto; Elizondo-Omaña, Rodrigo Enrique.
Título: Decellularization of human umbilical arteries / Descelularización de las arterias umbilicales humanas
Fonte: Int. j. morphol;37(1):111-117, 2019. tab, graf.
Idioma: en.
Resumo: SUMMARY: Arterial obstruction in small diameter (<6 mm) vessels are many times treated with grafts, however autologous aren't always available and synthetic have a high rate of complications. Decellularization of umbilical arteries may provide a solution, but the ideal method is debatable. We compare effectiveness between SDS and Triton X-100. Umbilical cords obtained from full term pregnancies with normal development and no evident complications in the newborn, were micro-dissected within 12 h and stored in phosphate buffered saline without freezing. Arteries were then processed for decellularization using 0.1 % and 1 % SDS, and 1 % Triton X100 protocols. Evaluation of cellular and nuclear material, collagen fibers, elastic fibers, and glycosoaminoglycans of the extracellular matrix (ECM) were evaluated as well as morphometric analysis under histological and immunohistochemical techniques. Triton X-100 was ineffective, preserving nuclear remains identified by immunofluorescence, had the most notable damage to elastic fibers, and decrease in collagen. SDS effectively eliminated the nuclei and had a less decrease in elastic fibers and collagen. Laminin was preserved in all groups. No significant differences were identified in luminal diameters; however the middle layer decreased due to decellularization of muscle cells. In conclusion, 0.1 % SDS decellularization was the most effective in eliminating cells and preserving the main components of the ECM.

RESUMEN: La obstrucción arterial en vasos de pequeño diámetro (<6 mm) se trata muchas veces con injertos, sin embargo, los autólogos no siempre están disponibles y los sintéticos tienen una alta tasa de complicaciones. La descelularización de las arterias umbilicales puede proporcionar una solución, pero el método ideal es discutible. Comparamos la efectividad entre los métodos SDS y Triton X-100. Cordones umbilicales obtenidos a partir de embarazos a término con evolución normal y sin complicaciones evidentes del recién nacido, se microdiseccionaron en 12 horas y se almacenaron en solución salina con fosfato sin congelación. Las arterias se procesaron luego para la descelularización usando los protocolos de SDS al 0,1 % y 1 %, y Triton X-100 al 1 %. Se realizó la evaluación de material celular y nuclear, fibras de colágeno, fibras elásticas y glucosoaminoglicanos de la matriz extracelular (MEC), así como el análisis morfométrico bajo técnicas histológicas e inmunohistoquímicas. Triton X-100 fue ineficaz, conservando los restos nucleares identificados por inmunofluorescencia, tuvo el daño más notable a las fibras elásticas y la disminución del colágeno. SDS efectivamente eliminó los núcleos y tuvo una disminución menor en las fibras elásticas y el colágeno. Laminina fue preservado en todos los grupos. No se identificaron diferencias significativas en los diámetros luminales; sin embargo, la capa media disminuyó debido a la descelularización de las células musculares. la descelularización con SDS al 0,1 % fue la más efectiva para eliminar células y preservar los principales componentes de la MEC.
Descritores: Artérias Umbilicais/citologia
Artérias Umbilicais/metabolismo
Engenharia Tecidual/métodos
Matriz Extracelular/metabolismo
-Artérias Umbilicais/transplante
Cordão Umbilical
Imuno-Histoquímica
Separação Celular
Imunofluorescência
Colágeno
Enxerto Vascular
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-838965
Autor: Mata-Miranda, Monica Maribel; Vazquez-Zapien, Gustavo Jesus; Rojas-Lopez, Marlon; Sanchez-Monroy, Virginia; Perez-Ishiwara, David Guillermo; Delgado-Macuil, Raul Jacobo.
Título: Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
Fonte: Biol. Res;50:14, 2017. tab, graf.
Idioma: en.
Resumo: BACKGROUND: Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells. RESULTS: In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. The second derivative of DKCs spectra showed changes in the protein bands compared to mPSCs. Finally, the principal components analysis obtained from FTIR spectra allowed to characterize chemical and structurally mPSCs and their differentiation process to DKCs in a rapid and non-invasive way. CONCLUSION: Our results indicated that we obtained DKCs from mPSCs, which passed through the chronological stages of embryonic kidney development. Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process.
Descritores: Diferenciação Celular/fisiologia
Espectroscopia de Infravermelho com Transformada de Fourier/métodos
Células-Tronco Pluripotentes/fisiologia
Rim/citologia
-Imuno-Histoquímica
Expressão Gênica
Células Cultivadas
Imunofluorescência
Análise de Componente Principal
Células-Tronco Pluripotentes/citologia
Reação em Cadeia da Polimerase em Tempo Real
Limites: Animais
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-954055
Autor: Kajbafzadeh, Abdol-Mohammad; Sabetkish, Shabnam; Sabetkish, Nastaran.
Título: The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney
Fonte: Int. braz. j. urol;44(3):608-616, May-June 2018. tab, graf.
Idioma: en.
Projeto: Tehran University of Medical Sciences.
Resumo: ABSTRACT Purpose: To identify the fetal stem cell (FSC) response to maternal renal injury with emphasis on renal integrity improvement and Y chromosome detection in damaged maternal kidney. Materials and Methods: Eight non-green fluorescent protein (GFP) transgenic Sprague-Dawley rats were mated with GFP-positive transgenic male rats. Renal damage was induced on the right kidney at gestational day 11. The same procedure was performed in eight non-pregnant rats as control group. Three months after delivery, right ne- phrectomy was performed in order to evaluate the injured kidney. The fresh perfused kidneys were stained with anti-GFP antibody. Polymerase chain reaction (PCR) assay was also performed for the Y chromosome detection. Cell culture was performed to detect the GFP-positive cells. Technetium-99m-DMSA renal scan and single-photon emission computed tomography (SPECT) were performed after renal damage induction and 3 months later to evaluate the improvement of renal integrity. Results: The presence of FSCs was confirmed by immune histochemical staining as well as immunofluorescent imaging of the damaged part. Gradient PCR of female rat purified DNA demonstrated the presence of Y-chromosome in the damaged maternal kidney. Moreover, the culture of kidney cells showed GPF- positive cells by immuno- fluorescence microscopy. The acute renal scar was repaired and the integrity of dam- aged kidney reached to near normal levels in experimental group as shown in DMSA scan. However, no significant improvement was observed in control group. Conclusion: FSC seems to be the main mechanism in repairing of the maternal renal injury during pregnancy as indicated by Y chromosome and GFP-positive cells in the sub-cultured medium.
Descritores: Cicatrização/fisiologia
Quimerismo
Células-Tronco Fetais/fisiologia
Nefropatias/fisiopatologia
Troca Materno-Fetal/fisiologia
-Fatores de Tempo
Cromossomo Y
Imuno-Histoquímica
Tomografia Computadorizada de Emissão de Fóton Único
Células Cultivadas
Reação em Cadeia da Polimerase
Imunofluorescência
Ratos Sprague-Dawley
Compostos Radiofarmacêuticos
Ácido Dimercaptossuccínico Tecnécio Tc 99m
Modelos Animais de Doenças
Nefropatias/patologia
Nefropatias/diagnóstico por imagem
Limites: Animais
Masculino
Feminino
Gravidez
Responsável: BR1.1 - BIREME


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Miglino, Maria Angélica
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Id: biblio-1134463
Autor: Barreto, Rodrigo S. N; de-Oliveira, Franceliusa Delys; Matias, Gustavo de Sá Schiavo; Rodrigues, Marcio N; Carvalho, Rafael C; Franciolli, André Luis R; Fratini, Paula; Miglino, Maria Angelica.
Título: Rabbit vomeronasal organ-derivered cells have mesenchymal profile and neuronal commitment / Las células derivadas del órgano vomeronasal del conejo tienen perfil mesenquimatoso y compromiso neuronal
Fonte: Int. j. morphol;38(5):1463-1472, oct. 2020. graf.
Idioma: en.
Projeto: São Paulo Research Foundation.
Resumo: SUMMARY: The vomeronasal organ (VNO) is an accessory organ involved on the olfactory pathway, that detects pheromones and emits signals in order to modulate social and reproductive behavior. The VNO stem cells replace neurons throughout life. The aim of this study was to isolate and characterize cells derived from the vomeronasal organ from New Zealand rabbits. Five male rabbits with 120 days were used for cell isolation and culture. Results: VNO-derived cells presented labelling for proliferation (PCNA), undifferentiated profile (Nanog), neuronal (GFAP), mesenchymal stem cells (CD73, CD90 and CD105 and Stro-1). Also, presence of cytoskeletal (Vimentin, b-tubulin and CK-18) and absence of hematopoietic markers (CD34, CD117 and CD45) both by immunofluorescence and flow cytometry. By PCR it was possible to verify the expression of some undifferentiated profile (Oct-4), neuronal (Nestin) and mesenchymal (CD73, CD105 and Vimentin) genes. Functionally, VNO-derived cells differentiate in vitro into adipocytes, osteocytes and chondrocytes, and presented no tumorigenic potential when injected to Balb/c nu/nu mice. In conclusion, the rabbit VNO-derived cells have a profile that could be supportive to VNO olfactory/neuroreceptor epithelium by delivering factors to epithelial turnover or even by differentiation into epithelial cells to replacement of commissural epithelium.

RESUMEN: El órgano vomeronasal (OVN) es un órgano accesorio de la vía olfatoria, que detecta feromonas y emite señales que afectan la modulación del comportamiento social y reproductivo. Las células madre OVN reemplazan las neuronas durante toda la vida. El objetivo de este estudio fue aislar y caracterizar células derivadas del órgano vomeronasal de conejos raza Nueva Zelanda. Para el aislamiento y el cultivo celular se utilizaron cinco conejos machos con una edad de 120 días. Las células del OVN presentaron etiquetado para la proliferación (PCNA), un perfil indiferenciado (Nanog), neuronal (GFAP), células madre mesenquimales (CD73, CD90 y CD105 y Stro-1). Además, se ob- servó presencia de citoesqueleto (Vimentina, β-tubulina y CK-18) y ausencia de marcadores hematopoyéticos (CD34, CD117 y CD45) tanto por inmunofluorescencia como por citometría de flujo. Me- diante PCR fue posible verificar la expresión de algunos genes de perfil indiferenciado (Oct-4), neuronal (Nestin) y mesenquimatoso (CD73, CD105 y Vimentin). Las células derivadas del OVN se diferencian in vitro en adipocitos, osteocitos y condrocitos, y no presentan un potencial tumorigénico al ser infiltrados en ratones Balb / c nu / nu. En conclusión, las células derivadas de OVN de conejo tienen un perfil que podría ser compatible con el epitelio olfatorio / neurorreceptor de OVN transmitiendo factores al recambio epitelial o incluso mediante la diferenciación en células epiteliales para reemplazar el epitelio comisural.
Descritores: Coelhos/anatomia & histologia
Órgão Vomeronasal/citologia
Células-Tronco Mesenquimais/fisiologia
-Bulbo Olfatório/citologia
Células-Tronco/fisiologia
Mucosa Olfatória/citologia
Reação em Cadeia da Polimerase
Imunofluorescência
Citometria de Fluxo
Neurônios/fisiologia
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Langoni, Hélio
Silva, Aristeu Vieira da
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Id: lil-718751
Autor: Silva, Dayse Vaniele da; Marques, Joil Moreira; Correa, Nelton Anderson Bespalez; Velasquez, Leonardo Garcia; Silva, Rodrigo Costa da; Langoni, Helio; Söhsten, Adriana Lebram von; Silva, Aristeu Vieira da.
Título: Evaluation of modified agglutination test and enzyme-linked immunosorbent assay in detection of toxoplasma gondii antibody in human sera / Avaliação do método de aglutinação direta e do ensaio imunoenzimático na detecção de anticorpos para toxoplasma gondii em soros humanos
Fonte: Arq. ciências saúde UNIPAR;16(1):17-20, jan-abr. 2012. tab, ilus.
Idioma: en.
Resumo: Toxoplasmosis is a zoonosis of high prevalence around the world, leading to serious congenital alterations and in immune compromised individuals. Its diagnosis is based in the detection of serum antibodies. This work aimed to compare serum antibody to Toxoplasma gondii detection by ELISA for IgG antibodies, and the MAT with the results of IFAT. From 73 samples examined by IgG-IFAT, 53 (72.6%) were positive, IgG-ELISA 55 (75.4%) and MAT, 58 (79.4%). No significative differences among results of tests are found. MAT shows high sensibility (100%) and lower specificity (75%), there were not false-negative results, but showed false-positives (25%). ELISA showed sensibility of 98.1% and specificity of 85,0%, false-negative rate of 1.9% and false-positive rate of 15.0%. MAT performance, when compared with IFAT, suggests the utilization of this method in diagnostic and epidemiological preliminary tests, besides ELISA has been used as a confirmatory test, for its specificity.

A toxoplasmose é uma zoonose de elevada prevalência em todo o mundo, podendo causar sérias alterações congênitas e em pacientes imunodeprimidos. Seu diagnóstico, na maioria das vezes, baseia-se na detecção de anticorpos séricos. Neste trabalho propôs-se comparar a detecção de anticorpos séricos para Toxoplasma pelo ELISA para IgG, e o MAD com os resultados da RIFI, em soros de indivíduos provenientes de um assentamento localizado em Eldorado, MS. Das 73 amostras examinadas pela RIFI-IgG, 53 (72,6%) amostras foram positivas, para o ELISA-IgG 55 (75,4%) e o MAD, 58 (79,4%). Não houve diferença significativa entre os resultados dos testes. O MAD apresentou alta sensibilidade (100%) e menor especificidade (75%), 25% de falso-positivos e sem resultados falso-negativos. Já o ELISA apresentou sensibilidade de 98,1% e especificidade de 85,0%, com taxa de falsos-negativos de 1,9% e de falso-positivos de 15,0%. A performance do MAD, quando comparado à RIFI, sugere a utilização deste método em provas de triagem diagnóstica e epidemiológica, enquanto que o ELISA seria utilizado como prova confirmatória, devido a sua especificidade.
Descritores: Ensaio de Imunoadsorção Enzimática
Toxoplasmose
Prevalência
Imunofluorescência
Aglutinação
Anticorpos
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-842843
Autor: Rodrigues, Ricardo Wilson de Pinho; Ribeiro, Afonso Bezerra; Berber, Gilcele de Campos Martin; Sheng, LeeYun; Damazo, Amilcar Sabino.
Título: Analysis of clinical data and T helper 1/T helper 2 responses in patients with different clinical forms of leprosy
Fonte: Rev. Soc. Bras. Med. Trop;50(2):208-215, Mar.-Apr. 2017. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo a Pesquisa de Mato Grosso; . FAPEMAT; . Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Abstract INTRODUCTION: Currently, there are no laboratory tests or sensitive and specific molecular markers for the early diagnosis of leprosy. The aim of this study was to analyze the clinical characteristics of patients with leprosy and investigate their immunological profile, comparing this with the type of lesion and the presence or absence of a Bacillus Calmette-Guérin (BCG) vaccination scar. METHODS: Statistical analyzes were performed by employing comparative tests (Pearson´s chi-square) to evaluate the variables in different clinical forms, considering significance at the 5% level. RESULTS: The study identified a predominance of lepromatous leprosy (26.9%) in patients aged between 34-53 years. Caucasians predominantly had borderline tuberculoid (BT) clinical forms (42%); a predominance of males with borderline lepromatous (19%) and lepromatous leprosy (26.9%) forms was observed; and the presence of BCG vaccination scars (27.5%) and lower limb nerves were more affected (38%) predominantly in the BT clinical form. Significant differences were identified, which included hypochromic lesions predominantly in the BT clinical form (24%); diffuse-type lesions predominantly in the tuberculoid (TT) clinical form (28%); ill-defined lesion border dominance in lepromatous leprosy (LL) clinical forms (30%); an irregular lesion limit predominantly in LL clinical forms (32%); and a predominant Th1 immune response in the BT clinical form (41.7%). CONCLUSIONS: The evaluation of the immunological profile in leprosy patients may contribute to the more detailed diagnosis and possibly better characterization of the prognosis for these individuals.
Descritores: Células Th2/imunologia
Células Th1/imunologia
Hanseníase Multibacilar/diagnóstico
Hanseníase Multibacilar/imunologia
Hanseníase Paucibacilar/diagnóstico
Hanseníase Paucibacilar/imunologia
-Biópsia
Estudos Transversais
Imunofluorescência
Células Th1/metabolismo
Hanseníase Multibacilar/classificação
Hanseníase Paucibacilar/classificação
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-897046
Autor: Corona, Thaila Francini; Böger, Beatriz; Rocha, Tatiana Carneiro da; Svoboda, Walfrido Külh; Gomes, Eliane Carneiro.
Título: Comparative analysis of Mouse Inoculation Test and Virus Isolation in Cell Culture for rabies diagnosis in animals of Parana, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;51(1):39-43, Jan.-Feb. 2018. tab.
Idioma: en.
Resumo: Abstract INTRODUCTION: Rabies is an acute zoonotic disease, caused by a rhabdovirus that can affect all mammals, and is commonly transmitted by the bite of a rabid animal. The definitive diagnosis is laboratorial, by the Fluorescent Antibody Test (FAT) as a quick test and Mouse Inoculation Test (MIT) as a confirmatory test (gold standard). Studies conducted over the past three decades indicate that MIT and Virus Isolation in Cell Culture (VICC) can provide the same effectiveness, the latter being considered superior in bioethics and animal welfare. The aim of this study was to compare VICC with MIT, in terms of accuracy, biosafety and occupational health, supply and equipment costs, bioethics and animal welfare, in a Brazilian public health lab. METHODS: We utilized 400 samples of animal neurological tissue to compare the performance of VICC against MIT. The variables analyzed were accuracy, biosafety and occupational health, time spent in performing the tests, supply and equipment costs, bioethics and animal welfare evaluation. RESULTS: Both VICC and MIT had almost the same accuracy (99.8%), although VICC presented fewer risks regarding biosafety and mental health of the technicians, and reduced time between inoculation and obtaining the results (approximately 22 days less). In addition, VICC presented lower supply costs (86.5% less), equipment costs (32.6% less), and the advantage of not using animals. CONCLUSIONS: These results confirm that VICC can replace MIT, offering the same accuracy and better features regarding cost, results, biosafety and occupational health, and bioethics and animal welfare.
Descritores: Raiva/diagnóstico
Vírus da Raiva/imunologia
Saúde do Trabalhador
Imunofluorescência/métodos
Técnicas de Cultura de Células/métodos
Contenção de Riscos Biológicos
Temas Bioéticos
-Vírus da Raiva/isolamento & purificação
Bem-Estar do Animal
Reprodutibilidade dos Testes
Fatores de Risco
Imunofluorescência/economia
Sensibilidade e Especificidade
Técnicas de Cultura de Células/economia
Custos e Análise de Custo
Camundongos
Limites: Animais
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: biblio-1134507
Autor: Salazar-de-Santiago, Alfredo; Avelar-González, Francisco J; Díaz, Juan Manuel; Campos-Navarro, Paloma M; Flores-Villalpado, Elizz M; Hernández-Cuéllar, Edgar E; Guerrero-Barrera, Alma L.
Título: Expression of enamel proteins in human dental pulp stem cells by the effect of extracellular matrix / Expresión de proteínas de esmalte en células madre de pulpa dental humana por el efecto de matriz extracelular
Fonte: Int. j. morphol;38(6):1742-1750, Dec. 2020. tab, graf.
Idioma: en.
Projeto: Federal Government of Mexico.
Resumo: SUMMARY: Mesenchymal stem cells are present in adult tissues such as the human dental pulp. They are pluripotent and can differentiate into various specialized cell types in vitro through appropriate stimuli. Ameloblasts produce human tooth enamel only during embryonic development before tooth eruption, so endogenous regeneration is not possible. Various efforts have been aimed at generating natural or artificial substitutes for dental enamel with properties similar to the specific components of said tissue. The purpose of this study was to induce human dental pulp stem cells to produce enamel proteins using extracellular matrix derived from the rat tail tendon and pigskin. Primary cultures of human dental pulp stem cells were established and characterized by RT-PCR and immunofluorescence, using mesenchymal cell markers such as CD14, CD40, CD44, CD105, and STRO-1. The cells were then incubated with the extracellular matrix for fourteen days and labeled with specific antibodies to detect the expression of dental enamel proteins such as amelogenin, ameloblastin, enamelisin, tuftelin, and parvalbumin, characteristics of the phenotype of ameloblasts. This work demonstrated a positive effect of the extracellular matrix to induce the expression of enamel proteins in the stem cells of the human dental pulp.

RESUMEN: Las células madre mesenquimales están presentes en los tejidos adultos como la pulpa dental humana. Son pluripotentes y pueden diferenciarse en varios tipos de células especializadas in vitro a través de estímulos adecuados. Los ameloblastos producen esmalte dental humano sólo durante el desarrollo embrionario antes de la erupción dental, por lo que no es posible su regeneración endógena. Varios esfuerzos se han orientado a generar sustitutos naturales o artificiales de esmalte dental con propiedades similares a los componentes específicos de este tejido. El propósito de este estudio fue inducir células madre de pulpa dental humana para producir proteínas del esmalte dental a través del estímulo de matriz extracelular derivada del tendón de la cola de rata y piel de cerdo. Se establecieron cultivos primarios de células madre de pulpa dental humana y se caracterizaron por RT-PCR e inmunofluorescencia utilizando marcadores de células mesenquimales como CD14, CD40, CD44, CD105 y STRO-1. Posteriormente, las células se incubaron con matriz extracelular durante un período de catorce días y se marcaron con anticuerpos específicos para detectar la expresión de proteínas de esmalte dental como amelogenina, ameloblastina, enamelisina, tuftelina y parvalbúmina, las cuales son características del fenotipo de ameloblastos. Este trabajo demostró el efecto positivo que tiene el empleo de la matriz extracelular para inducir la expresión de proteínas de esmalte en las células pluripotenciales de la pulpa dental humana.
Descritores: Células-Tronco
Proteínas do Esmalte Dentário
Polpa Dentária
Matriz Extracelular
-Imunofenotipagem
Imunofluorescência
Técnicas de Cultura de Células
Engenharia Tecidual
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central



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