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Id: lil-731978
Autor: Gozá Valdés, Roxana; Díaz Rodríguez, Raúl.
Título: Análisis genotípico de aislamientos de Mycobacterium tuberculosis en La Habana en 2009 / Genotyping of mycobacterium tuberculosis isolates in Havana, 2009
Fonte: Rev. cuba. med. trop;66(2):263-272, Mayo.-ago. 2014.
Idioma: es.
Resumo: INTRODUCCIÓN: la búsqueda de alternativas al polimorfismo de la longitud de los fragmentos de restricción (RFLP, siglas en inglés) con la sonda IS 6110 en la genotipificación de Mycobacterium tuberculosis ha propiciado el desarrollo de la tipificación con número variable de repeticiones en tándem de unidades repetitivas interespaciadas de micobacterias (MIRU-VNTR, siglas en inglés). OBJETIVO: evaluar la diseminación de genotipos de M. tuberculosis en La Habana en 2009. MÉTODOS: se estudiaron 80 aislamientos procedentes de unidades de salud durante 2009 y se caracterizaron por tipificación MIRU-VNTR-15. Los genotipos se expresaron como códigos numéricos según el número de copias de cada MIRU-VNTR amplificado, y se analizaron con la herramienta bioinformática en línea MIRU-VNTR plus. Se utilizó MIRU-VNTR-24 como tipificación secundaria en los aislamientos agrupados por MIRU-VNTR-15. RESULTADOS: con MIRU-VNTR-15 se definieron 41 genotipos diferentes; entre ellos, 33 únicos (41,25 por ciento), y ocho que agruparon a 47 aislamientos (58,75 por ciento). La tipificación MIRU-VNTR-24 logró diferenciar sólo el 5 por ciento de éstos, disminuyendo el porcentaje de agrupamiento a 53,75 por ciento. CONCLUSIONES: el elevado agrupamiento encontrado sugirió transmisión reciente, lo que pudo tener influencia en la incidencia de tuberculosis en La Habana en 2009(AU)

INTRODUCTION: the search for alternatives to restriction fragment length polymorphism (RFLP) with the IS6110probe for the genotyping of Mycobacterium tuberculosis has paved the way for the development of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing (MIRU-VNTR). OBJECTIVE: evaluate the spread of M. tuberculosis genotypes in Havana in 2009. METHODS: eighty isolates obtained from healthcare centers during 2009 were examined and characterized by 15-loci MIRU-VNTR typing. The genotypes were expressed as numerical codes according to the copy number of each amplified MIRU-VNTR, and they were analyzed with the online bioinformatic tool MIRU-VNTR plus. 24-loci MIRU-VNTR was used for secondary typing of isolates grouped by 15-loci MIRU-VNTR. RESULTS: forty-one different genotypes were defined with 15-loci MIRU-VNTR. Of these, 33 were single (41.25 percent), whereas 8 clustered 47 isolates (58.75 percent). Only 5 percent of the latter could be differentiated by 24-loci MIRU-VNTR, lowering the percentage of clustering to 53.75 percent. CONCLUSIONS: the high clustering values revealed by the study suggest that transmission was recent. This may have had an influence on the incidence of tuberculosis in Havana in 2009(AU)
Descritores: Tuberculose Pulmonar/epidemiologia
-Técnicas de Tipagem Bacteriana/métodos
Cuba
Perfil Genético
Limites: Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Elucir, Gir
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Id: lil-776534
Autor: Okado, Jessica Baleiro; Bogni, Simoni Camila; Reinato, Lílian Andreia Fleck; Martinez, Roberto; Gir, Elucir; Camargo, Ilana Lopes Baratella da Cunha.
Título: Molecular analysis of methicillin-resistant Staphylococcus aureus dissemination among healthcare professionals and/or HIV patients from a tertiary hospital
Fonte: Rev. Soc. Bras. Med. Trop;49(1):51-56, Jan.-Feb. 2016. graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); . Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Resumo: Abstract INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is a nosocomial pathogen in community settings. MRSA colonized individuals may contribute to its dissemination; the risk of MRSA infection is increased in human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS) patients, although the prevalence of colonization in this group is not well established. The present study addressed this issue by characterizing MRSA isolates from HIV/AIDS patients and their healthcare providers (HCPs) to determine whether transmission occurred between these two populations. METHODS: A total of 24 MRSA isolates from HIV-infected patients and five from HCPs were collected between August 2011 and May 2013. Susceptibility to currently available antimicrobials was determined. Epidemiological typing was carried out by pulsed-field gel electrophoresis, multilocus sequence typing, and Staphylococcus cassette chromosome (SCCmec) typing. The presence of heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) and heterogeneous daptomycin-resistant Staphylococcus aureus (hDRSA) was confirmed by population analysis profile. Isolates characterized in this study were also compared to isolates from 2009 obtained from patients at the same hospital. RESULTS: A variety of lineages were found among patients, including ST5-SCCmecII and ST30-SCCmecIV. Two isolates were Panton-Valentine leukocidin-positive, and hVISA and hDRSA were detected. MRSA isolates from two HCPs were not related to those from HIV/AIDS patients, but clustered with archived MRSA from 2009 with no known relationship to the current study population. CONCLUSIONS: ST105-SCCmecII clones that colonized professionals in 2011 and 2012 were already circulating among patients in 2009, but there is no evidence that these clones spread to or between HIV/AIDS patients up to the 7th day of their hospitalization.
Descritores: Infecções Estafilocócicas
Infecções por HIV/microbiologia
Infecção Hospitalar/transmissão
Transmissão de Doença Infecciosa do Profissional para o Paciente/estatística & dados numéricos
Staphylococcus aureus Resistente à Meticilina/genética
Antibacterianos/farmacologia
-Infecções Estafilocócicas/microbiologia
Testes de Sensibilidade Microbiana
Infecção Hospitalar/microbiologia
Técnicas de Tipagem Bacteriana
Eletroforese em Gel de Campo Pulsado
Epidemiologia Molecular
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Tipagem de Sequências Multilocus
Centros de Atenção Terciária
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-782105
Autor: Feyisa, Seifu Gizaw; Haeili, Mehri; Zahednamazi, Fatemeh; Mosavari, Nader; Taheri, Mohammad Mohammad; Hamzehloo, Gholamreza; Zamani, Samin; Feizabadi, Mohammad Mehdi.
Título: Molecular characterization of mycobacterium tuberculosis isolates from Tehran, Iran by restriction fragment length polymorphism analysis and spoligotyping
Fonte: Rev. Soc. Bras. Med. Trop;49(2):204-210, Mar.-Apr. 2016. tab, graf.
Idioma: en.
Projeto: Tehran University of Medical Sciences.
Resumo: Abstract: INTRODUCTION Characterization of Mycobacterium tuberculosis (MTB) isolates by DNA fingerprinting has contributed to tuberculosis (TB) control. The aim of this study was to determine the genetic diversity of MTB isolates from Tehran province in Iran. METHODS MTB isolates from 60 Iranian and 10 Afghan TB patients were fingerprinted by standard IS6110-restriction fragment length polymorphism (RFLP) analysis and spoligotyping. RESULTS The copy number of IS6110 ranged from 10-24 per isolate. The isolates were classified into 22 clusters showing ≥ 80% similarity by RFLP analysis. Fourteen multidrug-resistant (MDR) isolates were grouped into 4 IS6110-RFLP clusters, with 10 isolates [71% (95% CI: 45-89%)] in 1 cluster, suggesting a possible epidemiological linkage. Eighteen Iranian isolates showed ≥ 80% similarity with Afghan isolates. There were no strains with identical fingerprints. Spoligotyping of 70 isolates produced 23 distinct patterns. Sixty (85.7%) isolates were grouped into 13 clusters, while the remaining 10 isolates (14.2%) were not clustered. Ural (formerly Haarlem4) (n = 22, 31.4%) was the most common family followed by Central Asian strain (CAS) (n = 18, 25.7%) and T (n = 9, 12.8%) families. Only 1strain was characterized as having the Beijing genotype. Among 60 Iranian and 10 Afghan MTB isolates, 25% (95% CI: 16-37) and 70% (95% CI: 39-89) were categorized as Ural lineage, respectively. CONCLUSIONS A higher prevalence of Ural family MTB isolates among Afghan patients than among Iranian patients suggests the possible transmission of this lineage following the immigration of Afghans to Iran.
Descritores: Tuberculose/microbiologia
Variação Genética/genética
DNA Bacteriano/genética
Técnicas de Tipagem Bacteriana/métodos
Mycobacterium tuberculosis/genética
-Polimorfismo de Fragmento de Restrição
Análise por Conglomerados
Impressões Digitais de DNA
Epidemiologia Molecular
Genótipo
Irã (Geográfico)
Mycobacterium tuberculosis/isolamento & purificação
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1020442
Autor: Oliveira, Elaini Aparecida de; Paula, Geraldo Renato de; Mondino, Pedro Jose Juan; Chagas, Thiago Pavoni Gomes; Mondino, Sílvia Susana Bona de; Mendonça-Souza, Cláudia Rezende Vieira de.
Título: High rate of detection of OXA-23-producing Acinetobacter from two general hospitals in Brazil
Fonte: Rev. Soc. Bras. Med. Trop;52:e20190243, 2019. tab.
Idioma: en.
Resumo: Abstract INTRODUCTION In recent decades, the prevalence of carbapenem-resistant Acinetobacter isolates has increased, and the production of oxacillinase (OXA)-type carbapenemases is the main mechanism underlying resistance. We evaluated OXA production from 114 Acinetobacter isolates collected between March and December 2013 from different clinical specimens of patients in two hospitals (Hospital 1 [n = 61] and Hospital 2 [n = 53]) located in Niterói, Rio de Janeiro, Brazil. We also evaluated the genetic diversity of OXA-producing isolates. METHODS All the isolates were identified through the automated system Vitek II and matrix-assisted laser desorption ionization-time of flight mass spectrometry MALDI-TOF MS as belonging to the A. baumannii-A. calcoaceticuscomplex. Antimicrobial susceptibility profiles were verified through agar diffusion tests. The presence of OXA-encoding genes was confirmed by PCR. The genetic diversity of isolates positive for carbapenemase production was analyzed through pulsed-field gel electrophoresis. RESULTS There was a high rate of resistance to carbapenems in the isolates (imipenem: 96%; meropenem: 92%) from both hospitals. Moreover, a high percentage (95.6%) of OXA-23-positive isolates was observed for both hospitals, indicating that this was the main mechanism of carbapenem-resistance among the studied population. In addition, most isolates (96.5%) were positive for bla OXA-51. A high genetic diversity and a few major genotypes were found among the OXA-23-positive isolates analyzed. Only intra-hospital dissemination was observed. CONCLUSIONS The elevated dissemination of bla OXA-23-like observed among Acinetobacter isolates from both the studied hospitals highlights the need for continuous epidemiological surveillance in these institutions.
Descritores: Acinetobacter/enzimologia
beta-Lactamases/efeitos dos fármacos
Infecções por Acinetobacter/microbiologia
-Acinetobacter/efeitos dos fármacos
Acinetobacter/genética
beta-Lactamases/biossíntese
Brasil
DNA Bacteriano/genética
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase
Técnicas de Tipagem Bacteriana
Eletroforese em Gel de Campo Pulsado
Hospitais Gerais
Antibacterianos/farmacologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1057252
Autor: Anselmo, Lívia Maria Pala; Conceição, Emilyn Costa; Namburete, Evangelina; Feliciano, Cinara; Spindola, Silvana; Sola, Christophe; Bollela, Valdes Roberto.
Título: Mycobacterium tuberculosis spoligotypes and drug-resistant characterization from Beira compared to genotypes circulating in Mozambique
Fonte: Rev. Soc. Bras. Med. Trop;52:e20190257, 2019. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo; . University of Pittsburgh.
Resumo: Abstract INTRODUCTION Mozambique is one of three countries with high prevalence of tuberculosis (TB), TB/human immunodeficiency virus coinfection, and multidrug-resistant TB. We aimed to describe Mycobacterium tuberculosis spoligotypes circulating among drug resistant (DR) strains from Beira, Mozambique comparing them with genotypes in the country. METHODS: We performed spoligotyping of 79 M. tuberculosis suspected of DR-TB compared all spoligotype patterns published on the international database and PubMed. RESULTS: Both in Beira and Mozambique (n=578), the main clades were Latin-American-Mediterranean, East-African-Indian, Beijing and T, with no extensively DR TB cases. CONCLUSIONS: Beira and Mozambique share the same population genetic structure of M. tuberculosis.
Descritores: Variação Genética/genética
Tuberculose Resistente a Múltiplos Medicamentos/microbiologia
Mycobacterium tuberculosis/genética
-Filogenia
Técnicas de Tipagem Bacteriana
Genótipo
Moçambique
Mutação/genética
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-950825
Autor: Jurczak, Anna; Koscielniak, Dorota; Papiez, Monika; Vyhouskaya, Palina; Krzysciak, Wirginia.
Título: A study on ß-defensin-2 and histatin-5 as a diagnostic marker of early childhood caries progression
Fonte: Biol. Res;48:1-9, 2015. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: Recently, a continuous growth of interest has been observed in antimicrobial peptides (AMPs) in the light of an alarming increase in resistance of bacteria and fungi against antibiotics. AMPs are used as biomarkers in diagnosis and monitoring of oral cavity pathologies. Therefore, the determination of specific protein profiles in children diagnosed with early childhood caries (ECC) might be a basis for effective screening tests and specialized examinations which may enable progression of disease. METHODS: The objective of the studies was to determine the role of histatin-5 and ß-defensing-2 as a diagnostic marker of early childhood caries progression. In this work, results of concentration determination of two salivary proteins (histatin-5 and ß-defensin-2) were presented. In addition, bacterial profiles from dental plaque in various stages of ECC and control were marked. The assessment of alteration in the concentration of these two proteins in a study group of children with various stages of ECC and a control group consisting of children with no symptoms was performed by enzyme-linked immunosorbent assays. RESULTS: The statistical analysis showed a significant increase in the concentration of histatin-5 and ß-defensin-2 in the study group compared to the control group and correlated with the progression of the disease. CONCLUSIONS: The confirmation of concentration changes in these proteins during the progression of dental caries may discover valuable disease progression biomarkers.
Descritores: Saliva/química
beta-Defensinas/análise
Cárie Dentária/diagnóstico
Histatinas/análise
-Streptococcus/classificação
Streptococcus/crescimento & desenvolvimento
Ensaio de Imunoadsorção Enzimática
Biomarcadores/análise
Contagem de Colônia Microbiana
Transdução de Sinais
Modelos Lineares
Técnicas de Tipagem Bacteriana
Progressão da Doença
Cárie Dentária/microbiologia
Suscetibilidade à Cárie Dentária
Diagnóstico Precoce
Lactobacillus rhamnosus/crescimento & desenvolvimento
Anti-Infecciosos/análise
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Criança
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1171790
Autor: Asato Valeria C; Vilches Viviana E; Pineda María G; Casanueva Enrique; Cane Alejandro; Moroni Mirian P; Brengi Silvina P; Pichel Mariana G.
Título: First clinical isolates of Cronobacter spp. (Enterobacter sakazakii) in Argentina: characterization and subtyping by pulsed-field gel electrophoresis / First clinical isolates of Cronobacter spp. (Enterobacter sakazakii) in Argentina: characterization and subtyping by pulsed-field gel electrophoresis.
Fonte: Rev. argent. microbiol;45(3):160-4, set. 2013.
Idioma: es.
Resumo: Cronobacter species are opportunistic pathogens associated with severe infections in neonates and immunocompromised infants. From January 2009 through September 2010, two cases of neonatal infections associated with Cronobacter malonaticus and one case associated with Cronobacter sakazakii, two of them fatal, were reported in the same hospital. These are the first clinical isolates of Cronobacter spp. in Argentina. The objective of this work was to characterize and subtype clinical isolates of Cronobacter spp. in neonate patients, as well as to establish the genetic relationship between these isolates and the foodborne isolates previously identified in the country. Pulsed-field gel electrophoresis analysis showed a genetic relationship between the C. malonaticus isolates from two patients. Different results were found when the pulsed-field gel electrophoresis patterns of clinical isolates were compared with those deposited in the National Database of Cronobacter spp.
Descritores: Cronobacter sakazakii/classificação
Cronobacter sakazakii/isolamento & purificação
-Argentina
Eletroforese em Gel de Campo Pulsado
Feminino
Humanos
Masculino
Recém-Nascido
Técnicas de Tipagem Bacteriana
Tipo de Publ: Artigo de Revista
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes


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Id: biblio-1171768
Autor: Manias Valeria; Nagel Alicia; Mollerach Analía; Mendosa María A; Freyre Hugo; Gómez Abel; Ferrara Elisa; Vay Carlos; de Los A Méndez Emilce.
Título: Endocarditis por Brucella canis: primer caso documentado en un paciente adulto en Argentina / [Brucella canis endocarditis: first documented case in Argentina].
Fonte: Rev. argent. microbiol;45(1):50-3, mar. 2013.
Idioma: es.
Resumo: We herein present the case of an adult male patient who consulted for lower extremity edema, a 2- month history of fever and oppressive chest pain radiating to the left arm. He referred neither contact with breeding animals nor consumption of unpasteurized dairy products. A diagnosis of endocarditis was confirmed by cardiac studies. Since the empirical treatment with cephalotin, ampicillin and gentamicin failed, the patient underwent aortic valve replacement. A total of four blood cultures were positive with a gram-negative rod. Bacterial identification was performed using the API 20 NE technique (bioMÞrieux), the Phoenix automated method (BD) and conventional biochemical tests which were unable to classify the isolate as to genus and species. The strain was sent to the INEI-ANLIS "Dr. Carlos G. Malbrán" where it was identified as Brucella canis. The antimicrobial treatment was switched to doxycycline, rifampicin and trimethoprim-sulfamethoxazole with good evolution of the patient. The clinical significance of this case report lies in the possible failure of the empiric antibiotic therapy administered for endocarditis, since B. canis did not respond to the conventional antimicrobial treatment for this pathology.
Descritores: Brucella canis/isolamento & purificação
Brucelose/microbiologia
Endocardite Bacteriana/microbiologia
-Adulto
Argentina/epidemiologia
Bacteriemia/microbiologia
Brucella canis/efeitos dos fármacos
Brucelose/cirurgia
Brucelose/epidemiologia
Brucelose/tratamento farmacológico
Combinação Trimetoprima e Sulfametoxazol/uso terapêutico
Dor no Peito/etiologia
Doxiciclina/uso terapêutico
Edema/etiologia
Endocardite Bacteriana/cirurgia
Endocardite Bacteriana/epidemiologia
Endocardite Bacteriana/tratamento farmacológico
Farmacorresistência Bacteriana Múltipla
Febre/etiologia
Humanos
Implante de Prótese de Valva Cardíaca
Masculino
Rifampina/uso terapêutico
Terapia Combinada
Técnicas de Tipagem Bacteriana
Valva Aórtica/cirurgia
Valva Aórtica/microbiologia
Tipo de Publ: Relatos de Casos
Resumo em Inglês
Artigo de Revista
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes


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Id: biblio-1171767
Autor: Soloaga Rolando N; Carrión Natalia; Pagano Irene; Oviedo Claudia; Diez Adriana; Pidone Juan C; Almuzara Marisa; Vay Carlos; Galarza Patricia.
Título: Identificación de Neisseria gonorrhoeae. Utilidad de la tarjeta 2 NH del sistema Vitek 2C / [Neisseria gonorrhoeae identification. Usefulness of the Vitek 2C NH card].
Fonte: Rev. argent. microbiol;45(1):54-6, mar. 2013.
Idioma: es.
Resumo: A total of 115 unique clinical isolates of Neisseria gonorrhoeae and 54 strains of other genera and species included in the database of the NH card were tested by the Vitek 2C System (bioMÞrieux, Marcy L'Etoile, Francia). The gonoccocal isolates had been previously identified by conventional biochemical tests and by the latex agglutination test with monoclonal antibodies using the Phadebact Monoclonal GC Test (Bactus AB, Sweden). The NH card correctly identified 111 (96.5

) strains of 115 isolates; one strain was identified with low discriminatory power (0.86

) was misidentified (as Neisseria meningitidis) whereas the other two (1.7

) remained unidentified. The NH card for N. gonorrhoeae identification provided 100

specificity. The results were available within 6 hours. The NH card could be considered a reliable and useful tool for routine use in Neisseria gonorrhoeae identification.
Descritores: Gonorreia/microbiologia
Neisseria gonorrhoeae/isolamento & purificação
Técnicas de Tipagem Bacteriana/métodos
-Humanos
Kit de Reagentes para Diagnóstico
Reações Falso-Positivas
Sensibilidade e Especificidade
Testes de Fixação do Látex
Técnicas de Tipagem Bacteriana/instrumentação
Tipo de Publ: Estudo Comparativo
Resumo em Inglês
Artigo de Revista
Estudo de Validação
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes


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Texto completo
Id: biblio-1011408
Autor: Márquez, Sebastián L; Blamey, Jenny M.
Título: Isolation and partial characterization of a new moderate thermophilic Albidovulum sp. SLM16 with transaminase activity from Deception Island, Antarctica
Fonte: Biol. Res;52:5, 2019. tab, graf.
Idioma: en.
Resumo: BACKGROUND: A moderately thermophilic, slightly halophilic, aerobic, Gram-stain negative, bacterial strain, SLM16, was isolated from a mixed of seawater-sand-sediment sample collected from a coastal fumarole located in Whalers Bay, Deception Island, Antarctica. The aim was to screen for thermophilic microorganisms able to degrade primary amines and search for amine transaminase activity for potential industrial application. RESULTS: Identification and partial characterization of the microorganism SLM16 were carried out by means of morphological, physiological and biochemical tests along with molecular methods. Cells of strain SLM16 were non-motile irregular rods of 1.5-2.5 µm long and 0.3-0.45 µm wide. Growth occurred in the presence of 0.5-5.5% NaCl within temperature range of 35-55 °C and pH range of 5.5-9.5, respectively. The DNA G+C composition, estimated from ftsY gene, was 66% mol. Phylogenetic analysis using de 16S rRNA gene sequence showed that strain SLM16 belongs to the marine bacterial genus Albidovulum. CONCLUSION: Strain SLM16 is a moderate thermophilic Gram negative microorganisms which belongs to the marine bacterial genus Albidovulum and is closely related to Albidovulum inexpectatum species based on phylogenetic analysis. Additionally, amine-transaminase activity towards the arylaliphatic amine α-methylbenzylamine was detected.
Descritores: Água do Mar/microbiologia
DNA Bacteriano/genética
Rhodobacteraceae/isolamento & purificação
Rhodobacteraceae/enzimologia
Transaminases/metabolismo
-Filogenia
RNA Ribossômico 16S/genética
Técnicas de Tipagem Bacteriana
Análise de Sequência de DNA
Rhodobacteraceae/classificação
Regiões Antárticas
Responsável: CL1.1 - Biblioteca Central



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