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Id: biblio-1057401
Autor: Londero, Alejandra; Costa, Magdalena; Sucari, Adriana; Leotta, Gerardo.
Título: Comparación de tres técnicas para subtipificación molecular de Listeria monocytogenes / Comparison of three molecular subtyping techniques for Listeria monocytogenes
Fonte: Rev. argent. microbiol;51(4):359-362, dic. 2019. graf.
Idioma: en.
Resumo: Abstract Listeria monocytogenes is a foodborne pathogen. The recent alert for L. monocytogenes in vegetables from Argentina warns about the importance of reinforcing its isolation, characterization and subtyping in food, clinical and environmental samples. The aim of the present study was to compare the discriminatory power of enterobacterial repetitive interpower; genic consensus polymerase chain reaction (ERIC-PCR), automated ribotyping and pulsed-field gel electrophoresis (PFGE) to subtype strains of L. monocytogenes isolated from Argentine meat and environmental samples. Simpson's Diversity Index (DI) was calculated on the basis of based on the dendrograms obtained in the by cluster analysis, showing the following discriminatory power: ApaI-PFGE (0.980), AscI-PFGE (0.966), ribotyping (0.912), ERIC-PCR (0.886). The ID values between ApaI- and AscI-PFGE and between ribotyping and ERIC-PCR were not significantly different. Of the three techniques evaluated, PFGE showed the highest discriminatory power. However, the subtyping techniques should be accompanied by effective food monitoring strategies and reliable clinical and epidemiological studies.

Resumen Listeria monocytogenes es un patógeno alimentario. La reciente alerta por la presencia de L. monocytogenes en vegetales en Argentina advierte sobre la importancia de reforzar el aislamiento, la caracterización y la subtipificación de esta bacteria en muestras clínicas de alimentos y ambientales. El objetivo del presente estudio fue comparar el poder discriminatorio de enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), la ribotipificación automatizada y la pulsed-field gel electrophoresis (PFGE) para subtipificar cepas de L. monocytogenes aisladas de carne y de muestras ambientales en Argentina. El índice de diversidad (ID) de Simpson, calculado a partir de los dendrogramas obtenidos en el análisis de agrupamiento, mostró los siguientes resultados: Apal-PFGE (0,980), AscI-PFGE (0,966), riboti-pado (0,912), ERIC-PCR (0,886). Los valores obtenidos no fueron significativamente diferentes al comparar entre Apal- y AscI-PFGE, ni entre ribotipadoy ERIC-PCR. De las técnicas evaluadas, la PFGE presentó el mayor poder discriminatorio. Sin embargo, las técnicas de subtipificación deberían acompañarse de estrategias de control de los alimentos efectivas y de estudios clínicos y epidemiológicos confiables.
Descritores: Técnicas de Tipagem Bacteriana/métodos
Listeria monocytogenes/classificação
-Análise Discriminante
Ribotipagem/métodos
Listeria monocytogenes/isolamento & purificação
Tipo de Publ: Estudo Comparativo
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: lil-780824
Autor: Shirvan, Ali Nazari; Aitken, Robert.
Título: Isolation of recombinant antibodies directed against surface proteins of Clostridium difficile
Fonte: Braz. j. microbiol;47(2):394-402, Apr.-June 2016. tab, graf.
Idioma: en.
Resumo: Abstract Clostridium difficile has emerged as an increasingly important nosocomial pathogen and the prime causative agent of antibiotic-associated diarrhoea and pseudomembranous colitis in humans. In addition to toxins A and B, immunological studies using antisera from patients infected with C. difficile have shown that a number of other bacterial factors contribute to the pathogenesis, including surface proteins, which are responsible for adhesion, motility and other interactions with the human host. In this study, various clostridial targets, including FliC, FliD and cell wall protein 66, were expressed and purified. Phage antibody display yielded a large panel of specific recombinant antibodies, which were expressed, purified and characterised. Reactions of the recombinant antibodies with their targets were detected by enzyme-linked immunosorbent assay; and Western blotting suggested that linear rather than conformational epitopes were recognised. Binding of the recombinant antibodies to surface-layer proteins and their components showed strain specificity, with good recognition of proteins from C. difficile 630. However, no reaction was observed for strain R20291—a representative of the 027 ribotype. Binding of the recombinant antibodies to C. difficile M120 extracts indicated that a component of a surface-layer protein of this strain might possess immunoglobulin-binding activities. The recombinant antibodies against FliC and FliD proteins were able to inhibit bacterial motility.
Descritores: Proteínas de Bactérias/análise
Clostridioides difficile/genética
Infecções por Clostridium/microbiologia
Anticorpos Antibacterianos/análise
-Proteínas de Bactérias/genética
Proteínas de Bactérias/imunologia
Proteínas Recombinantes/análise
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Expressão Gênica
Western Blotting
Clostridioides difficile/isolamento & purificação
Clostridioides difficile/imunologia
Infecções por Clostridium/diagnóstico
Ribotipagem
Anticorpos Antibacterianos/genética
Anticorpos Antibacterianos/imunologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-426796
Autor: Avila-Campos, Mario J; Rivera, Irma N; Nakano, Viviane.
Título: Genetic diversity of oral Fusobacterium nucleatum isolated from patients with different clinical conditions
Fonte: Rev. Inst. Med. Trop. Säo Paulo;48(2):59-63, Mar,-Apr. 2006. ilus, tab.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: Neste estudo foi avaliada a diversidade genética de 23 amostras de Fusobacterium nucleatum isoladas da cavidade bucal de 15 pacientes com doença periodontal, de oito cepas isoladas de sete indivíduos sadios, de nove isoladas de nove pacientes com AIDS e de duas isoladas de dois macacos Cebus apella. Pela ação da enzima EcoRI sobre o DNA bacteriano foram reconhecidos 28 ribotipos agrupados de A a J. Os isolados testados formaram 24 ribotipos os quais foram contidos nos grupos A, B, C, D, E e F, e as três cepas de referência e dois isolados clínicos de A. actinomycetemcomitans e E. coli CDC formaram quatro diferentes ribotipos contidos nos grupos G, H, I e J. Em adição, as nove cepas de F. nucleatum isoladas de pacientes com AIDS, seis pertenciam ao grupo C e três ao grupo D. Usando-se a ribotipagem foi possível distinguir F. nucleatum isolados de diferentes origens.
Descritores: Variação Genética
DNA Bacteriano/análise
Infecções por Fusobacterium/microbiologia
Fusobacterium nucleatum/genética
Doenças Periodontais/microbiologia
-Infecções Oportunistas Relacionadas com a AIDS/microbiologia
Técnicas de Tipagem Bacteriana
Southern Blotting
Cebus/microbiologia
Ribotipagem
Limites: Humanos
Animais
Adolescente
Adulto
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-1000160
Autor: Fernández Figueiras, Sandra; Alonso Casanova, Guillermina.
Título: Tipificación molecular de aislados de vibrio cholerae O1 causantes de dos brotes de cólera en Venezuela / Molecular typing of vibrio cholerae O1 isolates causing two outbreaks of cholera in Venezuela
Fonte: Rev. Inst. Nac. Hig;48(1-2):82-98, 2017. tab, graf.
Idioma: es.
Resumo: En Venezuela, en junio de 1996, se reportó que los casos de cólera eran causados por V. cholerae O1 serotipo Ogawa. A finales de 1998 se detectó un segundo brote de cólera causado por V. cholerae O1 serotipo Inaba resistente a la ampicilina y el trimetoprim-sulfametoxazol. Para estudiar las relaciones entre las cepas se examinaron veinticinco aislados de Vibrio cholerae O1 obtenidos desde 1996 a 2000 en Venezuela, para determinar la presencia de genes de virulencia y perfiles genómicos. Mediante la reacción en cadena de la polimerasa se determinó la presencia de genes de virulencia. Para determinar el perfil genómico de los aislamientos se utilizó ribotipificación y electroforesis en gel de campo pulsado (PFGE). Todos los aislados resultaron positivos para los genes ctxA, ctxB, zot y ace. El análisis RFLP de los genes RNAr mostró un único patrón de ribotipo V. El análisis de PFGE mostró una similitud de 91,5% independientemente del año o lugar de aislamiento, lo que indica la relación genómica entre los aislados. En conjunto, los datos sugieren que la cepa de V. cholerae O1 resistente a los antibióticos que apareció en 1998 surgió de la cepa epidémica anterior o de otro estrechamente relacionado con el clon anterior, con cambio de serotipo y ganancia de determinantes de resistencia a antibióticos. Es muy importante monitorear continuamente la aparición de la variantes porque mejorará la comprensión de la evolución de nuevos clones de V. cholerae

In Venezuela, cholera reported in June 1996 was caused by V. cholerae O1 serotype Ogawa. Second outbreak of cholera caused by V. cholerae O1 serotype Inaba, resistant to ampicillin and trimethoprim- Sulfamethoxazole, was notify at the end of 1998. Twenty-five isolates of Vibrio cholerae O1 obtained from 1996 to 2000 in Venezuela were examined to study the relationships between strains, presence of virulence genes and genomic profiles. Presence of virulence genes was detected by Polymerase Chain Reaction. Ribotyping and pulsed-field gel electrophoresis (PFGE) were used to determine the genomic profile of isolates. All isolates shown PCR product for ctxA, ctxB, zot and ace genes. RFLP analysis of rRNA gene showed one unique pattern from ribotype V. PFGE analysis revealed a similarity of 91.5%, regardless year or place of isolation, suggesting genomic relatedness among them. Overall, these data suggest that antibiotic resistant V. cholerae O1 El Tor strain that appeared in 1998 emerged from the previous epidemic strain or from another closely related to the previous clone. It is important the continuous monitor the emergence of variants because it will improve our understanding of the evolution of new clones V. cholerae
Descritores: Vibrio cholerae
Cólera/epidemiologia
Ribotipagem
Tipagem Molecular
-Vibrio/química
Saúde Pública
Antibacterianos
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Estudo Observacional
Responsável: VE9.1 - Biblioteca


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Id: lil-784725
Autor: Pão, Evellyn Siqueira Rodrigues de; Moura, Laís Roberto Lopes dos Santos; Novais, Rogério Carlos.
Título: Detecção de Salmonella sp em carcaças de frango comercializadas na cidade de São Gonçalo, RJ / Detection of Salmonella in chicken carcasses commercialized in the city of Sao Goncalo, RJ
Fonte: Hig. aliment;29(246/247):118-122, jul.-ago. 2015. tab.
Idioma: pt.
Descritores: Aves Domésticas/microbiologia
Contaminação de Alimentos/análise
Microbiologia de Alimentos
Salmonella/isolamento & purificação
-Brasil
Amostras de Alimentos
Reação em Cadeia da Polimerase
Ribotipagem
Limites: Animais
Responsável: BR526.1 - Biblioteca de Saúde Pública


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Id: lil-741281
Autor: Abbas, Syed Zaghum; Riaz, Mehwish; Ramzan, Naseem; Zahid, M. Tariq; Shakoori, Farah R.; Rafatullah, Mohd..
Título: Isolation and characterization of arsenic resistant bacteria from wastewater
Fonte: Braz. j. microbiol;45(4):1309-1315, Oct.-Dec. 2014. ilus, graf, tab.
Idioma: en.
Resumo: The present study proposed the isolation of arsenic resistant bacteria from wastewater. Only three bacterial isolates (MNZ1, MNZ4 and MNZ6) were able to grow in high concentrations of arsenic. The minimum inhibitory concentrations of arsenic against MNZ1, MNZ4 and MNZ6 were 300 mg/L, 300 mg/L and 370 mg/L respectively. The isolated strains showed maximum growth at 37 ºC and at 7.0 pH in control but in arsenite stress Luria Bertani broth the bacterial growth is lower than control. All strains were arsenite oxidizing. All strains were biochemically characterized and ribotyping (16S rRNA) was done for the purpose of identification which confirmed that MNZ1 was homologous to Enterobacter sp. while MNZ4 and MNZ6 showed their maximum homology with Klebsiella pneumoniae. The protein profiling of these strains showed in arsenic stressed and non stressed conditions, so no bands of induced proteins appeared in stressed conditions. The bacterial isolates can be exploited for bioremediation of arsenic containing wastes, since they seem to have the potential to oxidize the arsenite (more toxic) into arsenate (less toxic) form.
Descritores: Antibacterianos/metabolismo
Arsênio/metabolismo
Farmacorresistência Bacteriana
Enterobacter/efeitos dos fármacos
Klebsiella pneumoniae/efeitos dos fármacos
Águas Residuárias/microbiologia
-Arsenitos/metabolismo
DNA Ribossômico/química
DNA Ribossômico/genética
Enterobacter/classificação
Enterobacter/crescimento & desenvolvimento
Enterobacter/isolamento & purificação
Concentração de Íons de Hidrogênio
Klebsiella pneumoniae/classificação
Klebsiella pneumoniae/crescimento & desenvolvimento
Klebsiella pneumoniae/isolamento & purificação
Testes de Sensibilidade Microbiana
Oxirredução
Proteoma/análise
Ribotipagem
/genética
RNA, RIBOSOMAL, ABNORMALITIES, MULTIPLES/genética
Temperatura
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-734583
Autor: Landone Vescovo, Ignacio A; Golemba, Marcelo D; Di Lello, Federico A; Culasso, Andrés C.A; Levinb, Gustavo; Ruberto, Lucas; Mac Cormack, Walter P; López, José L.
Título: Rich bacterial assemblages from Maritime Antarctica (Potter Cove, South Shetlands) reveal several kinds of endemic and undescribed phylotypes / Una rica colección de bacterias marinas antárticas (Caleta Potter, islas Shetlands del Sur) revela diversos filotipos endémicos y previamente no descritos
Fonte: Rev. argent. microbiol;46(3):218-230, oct. 2014. ilus, tab.
Idioma: en.
Resumo: .

Bacterial richness in maritime Antarctica has been poorly described to date. Phylogenetic affiliation of seawater free-living microbial assemblages was studied from three locations near the Argentinean Jubany Station during two Antarctic summers. Sixty 16S RNA cloned sequences were phylogenetically affiliated to Alphaproteobacteria (30/60 clones), Gammaproteobacteria(19/60 clones), Betaproteobacteria and Cytophaga-Flavobacteriia- Bacteroides (CFB), which were (2/60) and (3/60) respectively. Furthermore, six out of 60 clones could not be classified. Both, Alphaproteobacteria and Gammaproteobacteria, showed several endemic and previously undescribed sequences. Moreover, the absence of Cyanobacteria sequences in our samples is remarkable. In conclusion, we are reporting a rich sequence assemblage composed of widely divergent isolates among themselves and distant from the most closely related sequences currently deposited in data banks.
Descritores: Bactérias/isolamento & purificação
Água do Mar/microbiologia
-Regiões Antárticas
Sequência de Bases
Bactérias/classificação
Bactérias/genética
DNA Bacteriano/genética
DNA Ribossômico/genética
Evolução Molecular
Microbiota
Dados de Sequência Molecular
Filogenia
Ribotipagem
RNA Bacteriano/genética
/genética
RNA, RIBOSOMAL, ABNORMALITIES, MULTIPLES/genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Leal, Nilma Cintra
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Id: lil-722309
Autor: Rabelo, Marcelle Aquino; Bezerra Neto, Armando Monteiro; Loibman, Stéfany Ojaimi; da Costa Lima, Jailton Lobo; Ferreira, Ewerton Lucena; Leal, Nilma Cintra; Maciel, Maria Amélia Vieira.
Título: The occurrence and dissemination of methicillin and vancomycin-resistant Staphylococcus in samples from patients and health professionals of a university hospital in Recife, State of Pernambuco, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;47(4):437-446, Jul-Aug/2014. tab, graf.
Idioma: en.
Resumo: Introduction Methicillin-resistant Staphylococcus aureus (MRSA) strains have been responsible for many nosocomial outbreaks. Within hospitals, colonized employees often act as reservoirs for the spread of this organism. This study collected clinical samples of 91 patients admitted to the intensive care unit (ICU), hemodialysis/nephrology service and surgical clinic, and biological samples from the nasal cavities of 120 professionals working in those environments, of a University Hospital in Recife, in the State of Pernambuco, Brazil. The main objective of this study was to determine the occurrence and dissemination of methicillin- and vancomycin-resistant Staphylococcus spp. Methods The isolates obtained were tested for susceptibility to oxacillin and vancomycin and detection of the mecA gene. In addition, the isolates were evaluated for the presence of clones by ribotyping-polymerase chain reaction (PCR). Results MRSA occurrence, as detected by the presence of the mecA gene, was more prevalent among nursing technicians; 48.1% (13/27) and 40.7% (11/27) of the isolates were from health professionals of the surgical clinic. In patients, the most frequent occurrence of mecA-positive isolates was among the samples from catheter tips (33.3%; 3/9), obtained mostly from the hemodialysis/nephrology service. Eight vancomycin-resistant strains were found among the MRSA isolates through vancomycin screening. Based on the amplification patterns, 17 ribotypes were identified, with some distributed between patients and professionals. Conclusions Despite the great diversity of clones, which makes it difficult to trace the source of the infection, knowledge of the molecular and phenotypic profiles of Staphylococcus samples can contribute towards guiding therapeutic approaches in the treatment and control of nosocomial infections. .
Descritores: Antibacterianos/farmacologia
Infecção Hospitalar/microbiologia
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Oxacilina/farmacologia
Infecções Estafilocócicas/microbiologia
Resistência a Vancomicina
Vancomicina/farmacologia
-Brasil
Proteínas de Bactérias/genética
Infecção Hospitalar/diagnóstico
Infecção Hospitalar/transmissão
Pessoal de Saúde
Hospitais Universitários
Testes de Sensibilidade Microbiana
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Staphylococcus aureus Resistente à Meticilina/genética
Cavidade Nasal/microbiologia
Reação em Cadeia da Polimerase
Ribotipagem
Infecções Estafilocócicas/diagnóstico
Infecções Estafilocócicas/transmissão
Limites: Adulto
Feminino
Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-649925
Autor: Betanzos-Reyes, Ángel Francisco; González-Cerón, Lilia; Rodríguez, Mario Henry; Torres-Monzón, Jorge Aurelio.
Título: Seroepidemiología del paludismo en un grupo de migrantes en tránsito (Chiapas, 2008)
Fonte: Salud pública Méx;54(5):523-529, sept.-oct. 2012. graf, tab.
Idioma: es.
Resumo: OBJETIVO: Conocer la prevalencia del paludismo y los factores asociados con la infección de migrantes en la frontera sur de México, durante 2008. MATERIAL Y MÉTODOS: En 706 migrantes, se investigó la infección activa mediante prueba rápida y PCR o pasada, mediante serología y se aplicó un cuestionario para investigar las condiciones asociadas con la infección. RESULTADOS: 85.6% provenía de Centroamérica. Ninguno presentó infección activa; 4.2% fue seropositivo y la mayoría provenía de los países con mayor incidencia de paludismo en la región. La seropositividad se asoció con el número de episodios previos de paludismo (RM=1.44; IC95% 1.04-2.00), años de permanencia en su comunidad de origen (RM=1.03; IC95% 1.00 -1.07) y conocimiento y automedicación con antipalúdicos (RM=3.38; IC95% 1.48-7.67). CONCLUSIONES: La exposición previa de migrantes al paludismo y las dificultades para su detección indican la necesidad de nuevas estrategias para la vigilancia epidemiológica para estas poblaciones.

OBJECTIVE: To know the prevalence of malaria and the factors associated with the infection in migrants in the southern border of Mexico, during 2008. MATERIALS AND METHODS: In 706 migrants, active malaria infection was investigated using a rapid diagnostic test and PCR and past infection using serology. A questionnaire was applied to investigate the conditions associated to infection. RESULTS: 85.6% originated from Central America, none presented an active infection, although 4.2% were seropositive, most of these came from the countries with the highest malaria incidence in the region. Seropositivity was associated with the number of previous malaria episodes (OR=1.44; IC95% 1.04-2.00), years living in their community of origin (OR=1.03; IC95% 1.00-1.07), and knowledge and self-medication with anti-malaria drugs (OR=3.38; IC95% 1.48-7.67). CONCLUSIONS:. The previous exposure of migrants and the difficulties for their detection indicate the need of new strategies for the epidemiological surveillance for these populations.
Descritores: Emigração e Imigração
Malária/epidemiologia
Migrantes/estatística & dados numéricos
-África/etnologia
Anticorpos Antiprotozoários/sangue
Antimaláricos/uso terapêutico
Ásia/etnologia
América Central/etnologia
Culicidae/parasitologia
DNA de Protozoário/sangue
Mordeduras e Picadas de Insetos/prevenção & controle
Insetos Vetores/parasitologia
Controle de Mosquitos
Malária/sangue
Malária/diagnóstico
Malária/prevenção & controle
México/epidemiologia
Parasitemia/diagnóstico
Parasitemia/epidemiologia
Plasmodium falciparum/genética
Plasmodium falciparum/imunologia
Plasmodium vivax/genética
Plasmodium vivax/imunologia
Ribotipagem
Estudos Soroepidemiológicos
Fatores Socioeconômicos
Inquéritos e Questionários
América do Sul/etnologia
Limites: Adolescente
Adulto
Animais
Feminino
Humanos
Masculino
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-634703
Autor: SANTISO, GABRIELA; CHEDIAK, VIVIANA; MAIOLO, ELENA; MUJICA, MARÍA T; SAN JUAN, JORGE; ARECHAVALA, ALICIA; NEGRONI, RICARDO.
Título: Infección diseminada por Penicillium marneffei en un paciente HIV-positivo: Primera observación en la República Argentina / Disseminated infection due to Penicillium marneffei related to HIV infection: First observation in Argentina
Fonte: Rev. argent. microbiol;43(4):268-272, dic. 2011. ilus.
Idioma: es.
Resumo: Se presenta el primer caso humano de peniciliosis por Penicillium marneffei observado en la República Argentina. El paciente era un joven de 16 años, HIV-positivo, procedente de un área rural del sur de China. El paciente fue internado en el Hospital "F. J. Muñiz" por padecer una neumonía grave con insufciencia respiratoria aguda. El agente causal fue aislado de un lavado broncoalveolar y se lo observó en un citodiagnóstico de piel. La identifcación de P. marneffei fue confrmada por las características fenotípicas del aislamiento y la amplifcación del ADNr. El enfermo padecía una infección muy avanzada por HIV que condujo a la aparición simultánea de infecciones por citomegalovirus, Pneumocystis jirovecii y procesos bacterianos nosocomiales. Este complejo cuadro derivó en una evolución fatal.

The frst case observed in Argentina of AIDS-related human penicillosis is herein presented. The patient was a six- teen year-old young man coming from a rural area of southern China. He was admitted at the F. J. Muñiz Hospital of Buenos Aires city with severe pneumonia and adult respiratory distress. Penicillium marneffei was isolated from bronchoalveolar lavage fuid and was microscopically observed in a skin cytodiagnosis. P. marneffei identifcation was confrmed by rRNA amplifcation and its phenotypic characteristics. The patient suffered an advanced HIV infection and also presented several AIDS-related diseases due to CMV, nosocomial bacterial infections and Pneumocystis jirovecii which led to a fatal outcome.
Descritores: Infecções Oportunistas Relacionadas com a AIDS/microbiologia
Dermatomicoses/microbiologia
HIV-1
HIV-2
Pneumopatias Fúngicas/microbiologia
Penicillium/isolamento & purificação
-Infecções Oportunistas Relacionadas com a AIDS/epidemiologia
Argentina/epidemiologia
Líquido da Lavagem Broncoalveolar/microbiologia
China/etnologia
Infecções por Citomegalovirus/complicações
Diagnóstico Diferencial
DNA Fúngico/análise
Dermatomicoses/epidemiologia
Evolução Fatal
Histoplasmose/diagnóstico
Pneumopatias Fúngicas/diagnóstico
Pneumopatias Fúngicas/epidemiologia
Pneumocystis carinii
Reação em Cadeia da Polimerase
Penicillium/classificação
Pneumonia por Pneumocystis/complicações
Ribotipagem
Limites: Adolescente
Humanos
Masculino
Tipo de Publ: Relatos de Casos
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde