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Id: biblio-1284377
Autor: Oriani, Alejandra S; Gentili, Alejandro R; Baldini, Mónica D.
Título: Movilidad por sliding de Mycobacterium chubuense / Sliding motiliy in Mycobacterium chubuense
Fonte: Rev. argent. microbiol;48(1):91-92, mar. 2016. ilus.
Idioma: es.
Descritores: Mycobacterium/isolamento & purificação
Mycobacterium/crescimento & desenvolvimento
-Contagem de Colônia Microbiana/métodos
Crescimento Bacteriano/análise
Ágar/análise
Tipo de Publ: Ensaio Clínico
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-828943
Autor: Ciftci, Seyfettin; Nemut, Tijen; Culha, Mustafa Melih; Yilmaz, Hasan; Ustuner, Murat; Yavuz, Ufuk; Ozkan, Levend; Karadenizli, Aynur; Turkan, Sadi.
Título: Non-infected penile prosthesis cultures during revision surgery; comparison between antibiotic coated and non - coated devices
Fonte: Int. braz. j. urol;42(6):1183-1189, Nov.-Dec. 2016. tab.
Idioma: en.
Resumo: ABSTRACT Introduction: Aim of this study is to investigate bacterial growth on non-infected devices and compare antibiotic-coated and non-coated implants. Materials and methods: The charts of 71 patients who underwent revision surgeries for penile prosthesis between 1995 and 2013 were reviewed. Of those, 31 devices were antibiotic-coated prostheses, while 40 of the implants were non-coated. Swab cultures were routinely obtained from corporal, pump or reservoir site during the operation. If a bacterial biofilm was determined on the prosthesis, it was also cultured. Results: A total of 5 different organisms were cultured from 18 patients. Of them, 4 devices were antibiotic-coated and the other 14 were non-coated devices. Staphylococcus epidermidis was the most common organism, while Staphylococcus hominis, beta hemolitic streptococcus, Escherichia coli and Proteus mirabilis were also cultured. All patients who had positive cultures were treated with appropriate antibiotics for four weeks postoperatively. Median follow-up time was 41 months, ranging between 8 and 82 months. One prosthesis (non-coated) became clinically infected in the follow-up period with a totally different organism. Culture positivity rates of antibiotic-coated and non-coated devices were 13% and 35% respectively and the result was significant (p=0.00254). Conclusions: Positive bacterial cultures are present on non-infected penile prostheses at revision surgeries in some of the patients. Antibiotic coated prostheses have much less positive cultures than non-coated devices.
Descritores: Staphylococcus epidermidis/crescimento & desenvolvimento
Prótese de Pênis/microbiologia
Infecções Relacionadas à Prótese/prevenção & controle
Antibacterianos/administração & dosagem
-Staphylococcus epidermidis/efeitos dos fármacos
Fatores de Tempo
Contagem de Colônia Microbiana
Testes de Sensibilidade Microbiana
Prótese de Pênis/efeitos adversos
Células Cultivadas
Estudos Prospectivos
Estudos Retrospectivos
Infecções Relacionadas à Prótese/etiologia
Sistemas de Liberação de Medicamentos
Pessoa de Meia-Idade
Limites: Humanos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
Texto completo
Id: biblio-1012436
Autor: Figueiredo-Filho, Altamir Oliveira de; Bem, Jessica Silva Peixoto; Weber Sobrinho, Carlos Roberto; Souza, Fábio Barbosa de.
Título: Microbiological Water Evaluation from Biofilm Adhered to Dental Unit Waterlines / Evaluación Microbiológica del Agua de Biofilm Adherido a Líneas de Agua de la Unidad Dental
Fonte: Int. j. odontostomatol. (Print);13(3):357-362, set. 2019. tab.
Idioma: en.
Resumo: ABSTRACT: The contamination of the dental units' waterlines is a reality, which can develop individual and collective disorders. The aim of this study was to evaluate the prevalence and resistance profile of bacteria on the internal surfaces of waterlines in a dental clinic from a Dentistry school of a Brazilian university. The design was an exploratory, descriptive study with quantitative and qualitative approach. Samples (n=4) were collected for analysis at different points: the portion closest to the water reservoir of the chair, and the portion closest to the triple syringe. After collection the samples were cultured in BHI medium in an oven for 24-48 h at 37 °C. For the quantitative analysis 1 ml of each sample was used for serial dilution up to the dilution value seven. The colonies were counted after pour plate and the results expressed in UFC/cm2. The qualitative analysis was initiated with the cultivation of Agar Blood, EMB-Levine and Cetrimide Agar for 24 h, and the identification of bacteria was performed by microscopic analyses. The resistance profile was verified by classical antibiogram. The internal surfaces of unit waterlines units exhibited a mean of 2.44 x 109 CFU / cm2. Bacillus subtilis and Pseudomonas aeruginosa were identified. The resistance profile of Pseudomonas aeruginosa indicated sensitivity to all tested antibiotics. A large number of microorganisms was quantified from biofilm accumulated in the dental units' waterlines. However, they were not resistant to classic antibiogram. Better management and application of decontamination protocols for waterlines need to be applied since opportunistic infections may be associated with Pseudomonas aeruginosa.

RESUMEN: La contaminación de líneas de agua en las unidades dentarias es una realidad, generando enfermedades individuales y colectivas. El objetivo de este estudio fue evaluar la prevalencia y resistencia de las bacterias en las zonas internas de las líneas de agua de la Facultad de Odontología de una universidad brasileña. El diseño del estudio fue exploratorio, descriptivo con enfoques cuantitativos y cualitativos. Las muestras para análisis (n=4) fueron selecionadas de distintos lugares: el punto más cercano al sistema de agua del sillón odontológico y el punto más cercano a la jeringa tríplice. Las muestras obtenidas fueron cultivadas en un médio BHI por 24-48 h, en un horno a 37 ºC. Para el análisis cuantitativo, se utilizó 1 ml de cada muestra para dilución hasta el valor siete. Las colonias fueran contadas y los resultados fueron expresados en UFC/cm2. El análisis cualitativo fue iniciado con el cultivo de Agar Sangre, EBM-Levine y Agar Cetrimide por 24 h y la identificación de la bacteria fue realizada por análisis microscópicos. El perfil de resistencia fue verificado por el antibiograma clásico. Las zonas internas de las unidades de líneas de agua mostraron un promédio de 2,44 x 109 UFC/cm2. Bacillus subtilis y Pseudomonas aeruginosa fueron encontrados. El perfil de resistencia de Pseudomonas aeruginosa indicó sensibilidad a todos los antibióticos testados. Un gran número de microorganismos fue cuantificado desde la biopelícula acumulada en las líneas de agua de unidades dentales. Sin embargo, no resistieron al antibiograma clásico. Se requiere una mejor gestión y aplicación de protocolos de decontaminación en las líneas de agua debido a que las infecciones oportunistas puedan estar asociadas a Pseudomonas aeruginosa.
Descritores: Água/efeitos adversos
Biofilmes
Controle de Infecções Dentárias/métodos
-Microbiologia da Água
Brasil
Contagem de Colônia Microbiana
Contaminação de Equipamentos/prevenção & controle
Estudos Prospectivos
Equipamentos Odontológicos/microbiologia
Mycobacterium/crescimento & desenvolvimento
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
Teixeira, Lisete Ribeiro
ANTONANGELO, LEILA
Texto completo
Id: biblio-974919
Autor: Carnevale, Gabriela Gaspar; Vargas, Francisco Suso; Caiaffa-Filho, Hélio Hehl; Acencio, Milena Marques Pagliarelli; Marçal, Lia Junqueira; Sales, Roberta Karla Barbosa; Teixeira, Lisete Ribeiro; Antonangelo, Leila.
Título: Preanalytical conditions can interfere with M. tuberculosis detection by PCR in respiratory samples
Fonte: Clinics;73:e410, 2018. tab, graf.
Idioma: en.
Resumo: OBJECTIVES: Tuberculosis is one of the most prevalent infections in humans. Although culture is the reference for diagnosis, its sensitivity is compromised, especially in paucibacillary samples. Because polymerase chain reaction (PCR) amplifies mycobacterial DNA, it is more sensitive than culture for the diagnosis of Mycobacterium tuberculosis (Mtb). However, its performance can be affected by intrinsic sample inhibitors and by the extraction/detection techniques used. METHODS: We evaluated the influence of preanalytical conditions on Mtb detection in samples of sputum (SPU), bronchoalveolar lavage (BAL), and pleural fluid (PF) using combinations of extraction/detection methods. Respiratory samples were prepared to contain different concentrations of red blood cells and nucleated cells to which increasing amounts of Mtb colonies were inoculated and submitted to PCR. RESULTS: Up to 102 CFU/ml of Mtb were detected in the SPU in all methods, except for the Roche extraction/detection method, regardless of the preanalytical sample condition. In BAL samples, medium and high concentrations of cells and high concentrations of red blood cells contributed to a lower Mtb detection, regardless of the extraction method used. In PF, red blood cells were the variable that most interfered with Mtb detection, with better recovery (102 CFU/ml) observed with the Qiagen/Nanogen combination. CONCLUSION: The choice of Mtb extraction and detection method is of fundamental importance for PCR analytical sensitivity, especially when paucibacillary samples and/or samples containing potential PCR inhibitors are analyzed.
Descritores: Derrame Pleural/microbiologia
Escarro/microbiologia
Líquido da Lavagem Broncoalveolar/microbiologia
Reação em Cadeia da Polimerase/métodos
Mycobacterium tuberculosis/isolamento & purificação
-Tuberculose Pleural/microbiologia
DNA Bacteriano/isolamento & purificação
Contagem de Colônia Microbiana
Sensibilidade e Especificidade
Eritrócitos/microbiologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Texto completo
Id: biblio-950749
Autor: Yu, Guangchao; Chen, Lei; Lin, Chii-wann; Li, Bing; Cui, Hemiao; Chen, Siyi; Miao, Jian; Bian, Huawei; Chen, Dingqiang; Deng, Yang.
Título: Loop-mediated isothermal amplification assays for screening of bacterial integrons
Fonte: Biol. Res;47:1-10, 2014. ilus, tab.
Idioma: en.
Projeto: National 973-Plan of China; . International Science & Technology Cooperation Program; . Science and Technology Planning Project of Guangdong Province, China; . National Natural Science Foundation of China; . National Science and Technology Support Program; . National Outstanding Doctoral Dissertation Funding; . Guangdong Outstanding Doctoral Dissertation Funding; . China Postdoctoral Science Foundation funded; . Fundamental Research Funds for the Central Universities; . Fund for Outstanding Youth of Anhui Academy of Agricultural Sciences.
Resumo: BACKGROUND: The occurrence and prevalence of integrons in clinical microorganisms and their role played in antimicrobial resistance have been well studied recently. As screening and detection of integrons are concerned, current diagnostic methodologies are restricted by significant drawbacks and novel methods are required for integrons detection. RESULTS: In this study, three loop-mediated isothermal amplification (LAMP) assays targeting on class 1, 2 and 3 integrons were implemented and evaluated. Optimization of these detection assays were performed, including studing on the reaction temperature, volume, time, sensitivity and specificity (both primers and targets). Application of the established LAMP assays were further verified on a total of 1082 isolates (previously identified to be 397 integron-positive and 685 integron-negative strains). According to the results, the indispensability of each primer had been confirmed and the optimal reaction temperature, volume and time were found to be 65°C, 45 min and 25 µL, respectively. As application was concerned, 361, 28 and 8 isolates carrying intI1, intI2 and intI3 yielded positive amplicons, respectively. Other 685 integron-negative bacteria were negative for the integron-screening LAMP assays, totaling the detection rate and specificity to be 100%. CONCLUSIONS: The intI1-, intI2- and intI3-LAMP assays established in this study were demonstrated to be the valid and rapid detection methodologies for the screening of bacterial integrons.
Descritores: DNA Bacteriano/isolamento & purificação
Técnicas de Amplificação de Ácido Nucleico/métodos
Integrons
-Compostos Orgânicos
Salmonella/genética
Serratia marcescens/genética
Staphylococcus/genética
Vibrio cholerae/genética
Contagem de Colônia Microbiana
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase/métodos
Sensibilidade e Especificidade
DNA Complementar
Primers do DNA
Integrases/genética
Farmacorresistência Bacteriana/genética
Eletroforese em Gel de Ágar
Escherichia coli/genética
Corantes Fluorescentes
Temperatura Alta
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Queiroz, Margareth Maria de Carvalho
Zahner, Viviane
Texto completo
Id: biblio-1041502
Autor: Pereira, Lorrane de Andrade; Ferreira, Vitor dos Santos Baia; Leite, Nahara de Souza; Souza, Sandra Maria de Oliveira; Queiroz, Margareth Maria de Carvalho; Côrte-Real, Suzana; Zahner, Viviane.
Título: Larvicidal and adulticidal effects and ultrastructural changes of larvae midgut epithelium of musca domestica (diptera: muscidae) fed with bacillus thuringiensis var. kyushuensis
Fonte: Rev. Soc. Bras. Med. Trop;52:e20190135, 2019. graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento Pessoal de Nível Superior.
Resumo: Abstract INTRODUCTION: Musca domestica is resistant to many insecticides; hence, biological control is a suitable alternative. METHODS: We evaluated the lethality of strain Btk176 towards the larval and adult M. domestica and the histopathological effects in the larvae midgut. RESULTS: We observed 99% larval and 78.9% adult mortality within 48 hours of spore ingestion (dosage, 2.4×108 CFU/ml). The histopathological effects were consistent with cytotoxicity. PCR analysis showed the presence of the cry1Ba gene. Transmission electron microscopy revealed a bipyramidal parasporal body. Thurigiensin activity was not detected. CONCLUSIONS: The serovar, Btk176 might be a potential biocontrol agent for houseflies.
Descritores: Bacillus thuringiensis
Toxinas Bacterianas/farmacologia
Moscas Domésticas/efeitos dos fármacos
Inseticidas/farmacologia
Larva/efeitos dos fármacos
-Contagem de Colônia Microbiana
Controle Biológico de Vetores/métodos
Reprodutibilidade dos Testes
Análise de Variância
Microscopia Eletrônica de Transmissão
Exotoxinas
Limites: Animais
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-1041508
Autor: Herek, Thalita Caroline; Menegazzo, Vinícius Ribeiro; Ogaki, Mayara Baptistucci; Perini, Hugo Felix; Maia, Luciana Furlaneto; Furlaneto, Marcia Cristina.
Título: Biofilm formation by blood isolates of candida parapsilosis sensu stricto in the presence of a hyperglycidic solution at comparable concentrations of total parenteral nutrition
Fonte: Rev. Soc. Bras. Med. Trop;52:e20180182, 2019. tab, graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: Administration of total parenteral nutrition (TPN) via catheters increases the risk for candidemia from Candida parapsilosis. METHODS: C. parapsilosis sensu stricto blood isolates were evaluated for ability total biomass biofilm formation and morphogenesis in presence of glucose at TPN equivalent concentrations. RESULTS: Biofilms were increased at high glucose concentrations (25-30%) compared to the control medium. Significant increase in filamentous forms was observed in cultures with 30% glucose. CONCLUSIONS: Biofilm formation by C. parapsilosis sensu stricto in hyperglycidic medium may contribute to its pathogenic potential for fungemia related to TPN catheters.
Descritores: Biofilmes/crescimento & desenvolvimento
Candida parapsilosis/fisiologia
Glucose/farmacologia
-Contagem de Colônia Microbiana
Nutrição Parenteral Total no Domicílio
Biofilmes/efeitos dos fármacos
Meios de Cultura/química
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Texto completo
Id: biblio-1041546
Autor: Cruz, Andria Lopes; Souza, Fabiana Silva de; Abegg, Maxwel Adriano.
Título: Anti-Candida and anti-quorum sensing activity of airborne microorganisms detected by a rapid method
Fonte: Rev. Soc. Bras. Med. Trop;52:e20180152, 2019. tab, graf.
Idioma: en.
Projeto: Brazilian National Research Council.
Resumo: Abstract INTRODUCTION: Introducing new antibiotics to the clinic is critical. METHODS: We adapted a plate method described by Kawaguchi and coworkers in 20131 for detecting inhibitory airborne microorganisms. RESULTS: We obtained 51 microbial colonies antagonist to Chromobacterium violaceum, purified and retested them, and of these, 39 (76.5%) were confirmed. They comprised 24 bacteria, 13 fungi, and 2 yeasts. Among the fungi, eight (61.5%) produced active extracts. Among the bacterial, yeast, and fungal strains, 17 (44.7%) and 12 (31.6%) were active against Candida albicans and Candida parapsilosis, respectively. CONCLUSIONS: The proposed screening method is a rapid strategy for discovering potential antibiotic producers.
Descritores: Bactérias/isolamento & purificação
Candida/efeitos dos fármacos
Chromobacterium/efeitos dos fármacos
Microbiologia do Ar
Percepção de Quorum
Fungos/isolamento & purificação
Antibacterianos/isolamento & purificação
-Bactérias/metabolismo
Contagem de Colônia Microbiana
Fungos/metabolismo
Antibacterianos/metabolismo
Antibacterianos/farmacologia
Responsável: BR1.1 - BIREME


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Id: biblio-1085470
Autor: Arruda, Maria Sueli Pereira de; Richini, Virgínia Bodelão; Oliveira, S. M. A; Vilani-Moreno, Fátima Regina.
Título: Experimental murine mycobacteriosis: evaluation of the functional activity of alveolar macrophages in thalidomide- treated mice.
Fonte: Ribeirão Preto; s.n; 2004. 8 p. graf, tab.
Idioma: en.
Resumo: Thalidomide is a selective inhibitor of tumor necrosis factor-a (TNF-a), a cytokine involved in mycobacterial death mechanisms. We investigated the role of this drug in the functional activity of alveolar macrophages in the presence of infection induced by intranasal inoculation of Mycobacterium avium in thalidomide-treated and untreated adult Swiss mice. Sixty animals were inoculated with 5 x 106 M. avium by the respiratory route. Thirty animals received daily thalidomide (30 mg/kg mouse) and 30 received water by gavage up to sacrifice. Ten non-inoculated mice were used as a control group. Lots of animals from each group were evaluated until 6 weeks after inoculation. Infection resulted in an increased total number of inflammatory cells as well as increased activity of pulmonary macrophages. Histologically, intranasal inoculation of bacilli resulted in small mononuclear infiltrates located at the periphery of the organ. Culture of lung fragments revealed the presence of bacilli only at the beginning and at the end of the experimental period. Thalidomide administration did not affect the microbiological or histological features of the infection. Thalidomide-treated and untreated animals showed the same amount of M. avium colonies 3 weeks after infection. Although it did not affect bacillary clearance, thalidomide administration resulted in a decreased percent of spread cells and release of hydrogen peroxide, suggesting that factors other than TNF-a play a role in the killing of mycobacteria by alveolar macrophages. Thalidomide administration also reduced the number of spread cells among resident macrophages, suggesting a direct effect of the drug on this phenomenon
Descritores: Fator de Necrose Tumoral alfa
Imunossupressores
Macrófagos Alveolares
Mycobacterium avium
Talidomida
Tuberculose Pulmonar
-Contagem de Colônia Microbiana
Pulmão
Limites: Animais
Camundongos
Responsável: BR191.1 - Biblioteca e Centro de Documentação Luiza Keffer
BR191.1; 9470/s


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Id: biblio-1223492
Autor: Ortega, Silvia M; Britos, M. R; Sin, C. S; Caramello, C; Monzón, Javier.
Título: Prevalencia de Prevotella spp y Porphyromona spp en periodontitis crónica / Prevalence of Prevotella spp and Porphyromona spp in chronic periodontitis
Fonte: Rev. Fundac. Juan Jose Carraro;24(44):40-47, 2021. ilus, tab.
Idioma: es.
Resumo: Las enfermedades del periodonto tienen una etiopatogenia compleja y puede considerarse multifactorial. El factor etiológico esencial en la patología inflamatoria periodontal es la biopelícula dental y cuando el desequilibrio entre el huésped y los microorganismos cambia la complejidad de la flora. Ciertas bacterias como Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella loescheii, Fusobacterium nucleatum, Tannerrella forsythia, Campylobacter rectus, Eikenella corrodens y Treponema spp., han sido comúnmente relacionadas con la periodontitis crónica y son consideradas como indicadores de riesgo para la progresión de dicha enfermedad. El objetivo de este trabajo fue establecer la prevalencia de Prevotella spp y Porphyromona spp en los distintos estadios de periodontitis crónicas. Material y métodos: Se estudiaron 48 pacientes sistémicamente saludables con diagnóstico de periodontitis crónica. Se completó el consentimiento informado, se realizó historia clínica y examen periodontal. El estado periodontal se clasificó en distintos grados de severidad: leve, moderada y severa. Se tomaron muestras de dos sitios con mayor profundidad de sondaje con conos de papel absorbente estériles y se transportaron en un medio prerreducido. Para el aislamiento de Prevotella spp se utilizó agar Brucella más sangre ovina al 5%, hemina, vitamina K al que se agregaron vancomicina y kanamicina; Porphyromonas sp se aisló en el mismo medio con el agregado de bacitracina y colistina. Se sembraron 10 µl de muestra entera y las placas fueron incubadas en jarras de anaerobiosis por 5 a 7 días a 37ºC. Resultados: los distintos grados de periodontitis correspondieron a un 17% periodontits leve, 57% moderada y 26% severa. En el total de pacientes se determinó la presencia de Prevotella spp en el 54% de los casos y un 12,5% de Porphyromona spp. Conclusión: De los pacientes estudiados con periodontits crónica, un 52% correspondió al sexo masculino, un 57% de los casos correspondieron a periodontitis moderada. Se aisló Prevotella sp en todos los estadios de periodontitis crónica y Porphyromonas sp sólo en periodontitis severas (AU)

Periodontal diseases have a complex etiopathogenesis and can be considered multifactorial. The essential etiological factor in periodontal inflammatory pathology is the dental biofilm and when the imbalance between the host and the microorganisms changes the complexity of the flora. Certain bacteria such as Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella loescheii, Fusobacterium nucleatum, Tannerrella forsythia, Campylobacter rectus, Eikenella corrodens and Treponema spp., Have been commonly related to chronic periodontitis and are considered as risk indicators for the progression of said disease. The objective of this work was to establish the prevalence of Prevotella spp and Porphyromonas spp in the different stages of chronic periodontitis. Forty eight systemically healthy patients diagnosed with chronic periodontitis were studied. Informed consent was completed, a medical history and periodontal examination was carried out. The periodontal state was classified into different degrees of severity: mild, moderate and severe. Samples were taken from two sites with greater depth of probing with sterile absorbent paper cones and transported in a prereduced medium. For the isolation of Prevotella spp, Brucella agar plus 5% sheep blood, hemin, vitamin K to which vancomycin and kanamycin were added. For Porphyromonas spp, the same medium was used and bacitracin and colistin were added. 10 µl of the whole sample was seeded and the plates were incubated in anaerobic jars for 5 to 7 days at 37 ° C. Different degrees of periodontitis corresponded to 17% mild periodontitis, 57% moderate and 26% severe. In the total number of patients, the presence of Prevotella spp was determined in 54% of the cases and 12.5% of Porphyromona spp. Of the patients studied with chronic periodontitis, 52% corresponded to the male sex, 57% of the cases corresponded to moderate periodontitis. Prevotella spp was isolated in all stages of chronic periodontitis and Porphyromonas sp only in severe periodontitis (AU)
Descritores: Porphyromonas gingivalis/isolamento & purificação
Prevotella intermedia/isolamento & purificação
Periodontite Crônica/microbiologia
-Contagem de Colônia Microbiana
Fatores de Risco
Meios de Cultura
Placa Dentária/microbiologia
Distribuição por Idade e Sexo
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Responsável: AR29.1 - Biblioteca



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