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Id: biblio-1138560
Autor: Lipari, Flavio G; Hernández, Daniela; Vilaró, Mario; Caeiro, Juan P; Saka, Héctor Alex.
Título: Caracterización clínica, epidemiológica y microbiológica de bacteriemias producidas por enterobacterias resistentes a carbapenems en un hospital universitario de Córdoba, Argentina / Clinical, epidemiological and microbiological characterization of bacteremia produced by carbapenem-resistant enterobacteria in a university hospital in Córdoba, Argentina
Fonte: Rev. chil. infectol;37(4):362-370, ago. 2020. tab, graf.
Idioma: es.
Resumo: Resumen Introducción: Las enterobacterias son una causa principal de infecciones del torrente sanguíneo y su resistencia antimicrobiana se encuentra en aumento. Esto lleva a un incremento de la morbilidad-mortalidad y de los costos en la salud pública. Las enterobacterias resistentes a carbapenems representan un grave desafío a nivel global ya que existen escasas opciones terapéuticas disponibles. Objetivo: Caracterización clínico/microbiológica de las bacteriemias resistentes a carbapenémicos observadas en un período de 4 años. Material y Método: Estudio retrospectivo, observacional y descriptivo, sobre las bacteriemias por enterobacterias resistentes y sensibles a carbapenems. Resultados: Se analizó un total de 84 pacientes con bacteriemia por enterobacterias resistentes y sensibles a carbapenems. Entre las resistentes, observamos una mayor proporción de: tratamiento antimicrobiano previo, hospitalización en unidad de terapia intensiva (UTI), inicio de la bacteriemia en UTI y antecedentes de β-lactamasas de espectro extendido. Además, se detectó un amplio predominio de Klebsiella pneumoniae productor de KPC y una mortalidad atribuible de 52,4%. Discusión: El estudio posibilitó profundizar el conocimiento de una enfermedad emergente de elevada mortalidad, en vistas al diseño y aplicación de estrategias de control de infecciones y de esquemas de tratamiento efectivos adaptados a la epidemiologia local.

Abstract Background: Enterobacteriaceae are a major cause of bloodstream infections and their antimicrobial resistance continues to increase. This leads to higher morbidity-mortality rates and public health costs. Carbapenem-resistant Enterobacteriaceae represent a serious challenge globally, since there are few therapeutic options available. Aim: Clinical/microbiological characterization of the carbapenem-resistant bacteremia observed over a period of 4 years. Methods: Retrospective, observational and descriptive study about bacteremia caused by carbapenem-resistant and susceptible Enterobacteriaceae. Results: A total of 84 patients with bacteremia including carbapenem-resistant and susceptible Enterobacteriaceae were analyzed. We found that patients infected with carbapenem-resistant strains presented a higher proportion of: previous antibiotic treatment, hospitalization in intensive care unit (ICU), onset of the bacteremia during hospitalization in ICU and previous infection with extended-spectrum-beta-lactamase producing Enterobacteriaceae. Additionally, we observed a predominance of KPC-producing Klebsiella pneumoniae and an attributable mortality rate of 52.4%. Discussion: This study allowed for a better understanding of an emerging problem with high mortality, which in turn is useful for the design and adoption of infection control strategies and effective treatment regimens adapted to our local epidemiology.
Descritores: Infecções por Klebsiella/tratamento farmacológico
Infecções por Klebsiella/epidemiologia
Bacteriemia/tratamento farmacológico
Bacteriemia/epidemiologia
-Argentina/epidemiologia
beta-Lactamases
Testes de Sensibilidade Microbiana
Carbapenêmicos/farmacologia
Estudos Retrospectivos
Farmacorresistência Bacteriana
Enterobacteriaceae
Klebsiella pneumoniae
Antibacterianos/uso terapêutico
Antibacterianos/farmacologia
Limites: Humanos
Tipo de Publ: Estudo Observacional
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1138563
Autor: Gaete, Margarita Enberg; Valenzuela, Marcela Puente; Bachero, Aniela Wozniak; Vega, Claudia Castillo; Marín, Nicolás Villagra; Labarca, Jaime Labarca; Cañete, Patricia García.
Título: Carbapenemasas en Pseudomonas aeruginosa con susceptibilidad disminuida a los carbapenémicos después de una década, desde VIM a KPC / Carbapenemases in Pseudomonas aeruginosa with decreased susceptibility to carbapenems after a decade: from VIM to KPC
Fonte: Rev. chil. infectol;37(4):389-394, ago. 2020. tab, graf.
Idioma: es.
Resumo: Resumen Introducción: Pseudomonas aeruginosa es relevante en infecciones asociadas a la atención de salud, principalmente cuando presenta resistencia a carbapenémicos. Objetivos: Estudiar la producción de carbapenemasas en P. aeruginosa, con susceptibilidad disminuida a carbapenémicos procesadas en el Laboratorio de Microbiología de la Red de Salud UC-CHRISTUS entre 2014-2015, y compararlas con las cepas estudiadas en 2004-2005. Métodos: Entre enero de 2014 y junio de 2015, se aislaron 459 cepas de P. aeruginosa provenientes de muestras clínicas. La susceptibilidad fue determinada por dilución en agar y a las cepas con susceptibilidad disminuida a carbapenémicos se les realizó test de carbaNP. Las cepas positivas fueron estudiadas por RPC para genes blaVIM, blaVIM-1, blaVIM-2, blaIMP, blaNDM, blaKPC, blaOXA y blaIMI. Se realizó en cepas seleccionadas electroforesis de campo pulsado. Resultados: De las 459 cepas estudiadas, 300 presentaban susceptibilidad disminuida a carbapenémicos (65,3%). De éstas, 183 fueron viables para estudio, correspondientes a 164 pacientes. El test de carbaNP fue positivo en 44 cepas de las 183 cepas (24%). Los genes de resistencia encontrados fueron: blaVIM-2 en 35 cepas, blaKPC-2+VIM-2 en 7 cepas y blaKPC-2 en 2 cepas. En las cepas blaKPC-2 se encontró relación clonal entre ellas. Conclusiones: Un 65,3% de P. aeruginosa presentó susceptibilidad disminuida a carbapenémicos, observándose que la presencia de carbapenemasas no es el principal mecanismo de resistencia. Además, se describe la emergencia en Chile de cepas de P. aeruginosa con carbapenemasas del tipo KPC-2 sola o en combinación con VIM-2.

Abstract Background: Pseudomonas aeruginosa is a relevant infectious agent affecting patients within health care setting; this situation is worsening with the appearance of strains resistance to carbapenems. Aims: To study carbapenemase production in P. aeruginosa with decreased susceptibility to carbapenems processed in the microbiology laboratory of the Health Network UC-CHRISTUS in 2014-2015 and compare them with the strains studied in 2004-2005. Methods: Between January 2014 and June 2015, 459 strains of P. aeruginosa from clinical samples were isolated. Susceptibility was determined by dilution in agar and strains with reduced susceptibility to carbapenems were tested for carbaNP. Positive strains were studied by PCR for blaVIM, blaVIM-1, blaVIM-2, blaIMP, blaNDM, blaKPC, blaOXA and blaIMI genes. Pulsed field electrophoresis was performed on selected strains. Results: From 459 strains studied, 300 had reduced susceptibility to carbapenems (65.3%). Of these, 183 were viable for study, corresponding to 164 patients. The carbaNP test was positive in 44 strains of the 183 strains (24%). The resistance genes found were: blaVIM-2 in 35 strains, blaKPC-2+VIM-2 in 7 strains and blaKPC-2 in 2 strains. In the blaKPC-2 strains clonal relation between them was found. Conclusions: A 65.3% of P. aeruginosa presented decreased susceptibility to carbapenems being the presence of carbapenemases not the main resistance mechanism. In addition, the emergence in Chile of P. aeruginosa strains with bla of the KPC-2 type alone or in combination with VIM-2 is described.
Descritores: Pseudomonas aeruginosa/genética
Carbapenêmicos/farmacologia
-Proteínas de Bactérias/genética
beta-Lactamases
Testes de Sensibilidade Microbiana
Chile
Antibacterianos/farmacologia
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1138564
Autor: Quino, Willi; Hurtado, Carmen V; Meza, Ana María; Zamudio, María Luz; Gavilan, Ronnie G.
Título: Patrones de resistencia a los antimicrobianos en serovares de Salmonella enterica en Perú, 2012-2015 / Patterns of resistance to antimicrobials in serovars of Salmonella enterica in Peru, 2012-2015
Fonte: Rev. chil. infectol;37(4):395-401, ago. 2020. tab, graf.
Idioma: es.
Resumo: Resumen Introducción: La salmonelosis es una zoonosis universal, causante de frecuentes brotes de enfermedades transmitidas por alimentos; Salmonella enterica es la especie con la mayor prevalencia, describiéndose un aumento progresivo de su resistencia a antimicrobianos. Objetivo: Determinar la frecuencia de serotipos y los patrones de resistencia antimicrobiana en aislados de S. enterica remitidos al Instituto Nacional de Salud, Lima, Perú. Materiales y Métodos: Se realizó un estudio descriptivo, transversal. Se incluyeron en el estudio todas las cepas remitidas como parte de la vigilancia nacional basada en laboratorio entre los años 2012 y 2015. Las cepas fueron confirmadas mediante pruebas convencionales y serotipificadas por el esquema de Kauffmann-White; la susceptibilidad antimicrobiana y la confirmación del fenotipo BLEE se realizó según el método de Kirby-Bauer y método de Jarlier. Resultados: Un total de 540 cepas de S. enterica fueron incluidos en el estudio, de las que 96% (520/540) correspondió a cepas de origen humano y 4% (20/540) de origen no humano (aves, alimentos y ambiental). En muestras humanas, el serovar más frecuente fue S. Infantis (57%), seguido de S. Enteritidis (27%) y S. Typhimurium (6%). Se encontró una alta resistencia a nitrofurantoína (74%), ácido nalidíxico (64%), ciprofloxacina (63%), tetraciclina (63%), ampicilina (56%), cotrimoxazol (56%), cefotaxima (53%) y cloranfenicol (50%). En muestras no humanas, el serotipo más frecuente fue S. Infantis (45%), seguido de S. Typhimurium (40%) y S. Enteritidis (10%). encontrándose una alta resistencia a ciprofloxacina (45%), cotrimoxazol (40%), y tetraciclina (40%). El 65% del total de las cepas presentó resistencia a más de dos antimicrobianos, 43,3% fueron productoras de BLEE y 99% de éstas presentaron resistencia a entre seis y ocho antimicrobianos. Conclusiones: Se encontró una alta frecuencia de Salmonella Infantis productoras de BLEE, con multi-resistencia a los antimicrobianos en los aislados de muestras humanas y no humanas recibidas en el Instituto Nacional de Salud.

Abstract Background: Salmonellosis is a universal zoonosis, causing frequent outbreaks of foodborne illness; Salmonella enterica is the species with the highest prevalence, a progressive increase in its resistance to antimicrobials is described. Aim: To determine the frequency of serovars and antimicrobial resistance patterns in S. enterica isolates submitted to the National Institute of Health, Lima, Peru. Methods: This is a cross-sectional study. All strains referred as part of national laboratory-based surveillance between 2012 and 2015 were included in the study. Strains were confirmed by conventional tests and serotyped by the Kauffmann-White scheme; antimicrobial susceptibility and confirmation of the BLEE phenotype was performed according to the method of Kirby-Bauer and Jarlier's method. Results: A total of 540 strains of S. enterica were included in the study, where 96% (520/540) corresponded to human strains and 4% (20/540) to non-human strains (birds, food and environmental). In human samples, the most frequent serovar was S. Infantis (57%), followed by S. Enteritidis (27%) and S. Typhimurium (6%). High resistance to nitrofurantoin (74%), nalidixic acid (64%), ciprofloxacin (63%), tetracycline (63%), ampicillin (56%), sulfamethoxazole-trimethoprim (56%), cefotaxime (53%) and chloramphenicol (50%) was detected. In non-human samples, the most frequent serotype was S. Infantis (45%), followed by S. Typhimurium (40%) and S. Enteritidis (10%); a high resistance to nalidixic acid (55%), ciprofloxacin (45%), sulfamethoxazole-trimethoprim (40%), nitrofurantoin (40%), tetracycline (40%) was found. 65% of all strains had resistance to more than two antibiotics, 43,3% were ESBL producers and 99% of these had resistance between six and eight antibiotics. Conclusions: We found a high frequency of S. Infantis producing ESBL with multi-resistance to the antimicrobials in human and nonhuman samples received by the National Institute of Health.
Descritores: Salmonella enterica/efeitos dos fármacos
-Peru/epidemiologia
Testes de Sensibilidade Microbiana
Estudos Transversais
Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos
Sorogrupo
Antibacterianos/farmacologia
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1144246
Autor: Jover-García, Jorge; Gil-Tomás, Jesús J; Díaz-Lantada, Andrés; Lafont-Morgado, Pilar; Oliver-Sáez, Paloma; Colomina-Rodríguez, Javier.
Título: Validación de un dispositivo point-of-care para la detección rápida de infección urinaria y susceptibilidad antimicrobiana / Validation of point-of-care device for rapid detection of urinary tract infection and antibiotic susceptibility
Fonte: Rev. chil. infectol;37(5):523-530, nov. 2020. tab, graf.
Idioma: es.
Resumo: Resumen Introducción: Las infecciones del tracto urinario (ITU) presentan una elevada prevalencia en el ámbito comunitario. Un rápido diagnóstico microbiológico es esencial para asegurar una terapia adecuada y efectiva. Objetivo: Evaluar un kit de antibiograma rápido (KAR®) en formato point-of-care para la detección rápida de ITU y sensibilidad antimicrobiana. Material y Métodos: El dispositivo KAR® se diseñó y desarrolló en colaboración con ingenieros técnicos y microbiólogos clínicos. Su evaluación se realizó a través de un estudio multicéntrico en el que participaron tres hospitales españoles. Para ello, se realizaron distintos ensayos in vivo con el fin de determinar la correlación del dispositivo con las técnicas microbiológicas de referencia. Resultados: Se ensayó un total de 400 muestras de orinas procedentes de pacientes con sospecha de ITU. El dispositivo KAR® proporcionó rápidos resultados (tiempo medio de positividad de 7,8 ± 1,5 h) con 97% de sensibilidad, 89% de especificidad y 87% de concordancia para la detección de bacteriuria significativa. Los porcentajes de especificidad para los antimicrobianos testados fueron: ciprofloxacina (97%), fosfomicina (94%), cotrimoxazol (84%), ampicilina (80%) y amoxicilina/ácido clavulánico (55%). Conclusión: El dispositivo KAR® puede ser una herramienta útil para el diagnóstico de ITU en pacientes ambulatorios, especialmente en áreas de bajo nivel socio-económico.

Abstract Background: Urinary tract infections (UTI) presents a high prevalence in the community setting. Rapid and accurate microbiological diagnosis is essential to ensure adequate and effective therapy. Aim: To evaluate a rapid antibiogram kit (KAR®) in point-of-care format for rapid detection of UTI and antibiotic susceptibility. Methods: The KAR® device has been designed and developed in collaboration with technical engineers and clinical microbiologists. Its evaluation has been carried out through a multicenter study in which three Spanish hospitals have participated. Thus, different in vivo tests have been implemented in order to determine device correlation with the reference microbiological techniques. Results: During the study period, a total of 400 urine samples from patients with suspected ITU were tested. The KAR® device provided fast results (mean positivity time of 7,8 ± 1,5 hours) with 97% sensitivity, 89% specificity and 87% agreement for the detection of significant bacteriuria. The percentages of specificity for the antibiotics tested were: ciprofloxacin (97%), fosfomycin (94%),cotrimoxazole (84%), ampicillin (80%) and amoxicillin/clavulanic acid (55%). Conclusion: The KAR® device could be a useful tool for diagnosing UTI in outpatients, especially in areas of low socio-economic level.
Descritores: Infecções Urinárias/diagnóstico
Infecções Urinárias/tratamento farmacológico
Sistemas Automatizados de Assistência Junto ao Leito
-Testes de Sensibilidade Microbiana
Prevalência
Antibacterianos/uso terapêutico
Limites: Humanos
Tipo de Publ: Estudo Multicêntrico
Estudo de Validação
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1135565 LILACS-Express
Autor: Araújo, Thiara Karine de; Costa, Edja Maria Melo de Brito; Maia, Carolina Medeiros de Almeida; Alves, Pollianna Muniz; Nonaka, Cassiano Francisco Weege; Silva, Priscilla Guimarães; Lima, Rennaly de Freitas; Godoy, Gustavo Pina.
Título: Chemical Composition, Antibacterial and Antifungal Potential of an Extract From the Leaves of Guapira Graciliflora Mart. Against Oral Microorganisms of Dental Interest
Fonte: Pesqui. bras. odontopediatria clín. integr;20:e5165, 2020. tab.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Abstract Objective: To perform an in vitro analysis of antibacterial and antifungal potential of an alcoholic extract from the leaves of Guapira Graciliflora Mart. against oral microorganisms and determine its chemical composition. Material and Methods: A hydroalcoholic extract of the leaves form G. graciliflora was obtained through maceration, vacuum concentration and freeze-drying. Antibacterial and antifungal activities were evaluated against Streptococcus mutans, Streptococcus salivarius, Streptococcus oralis, Streptococcus parasanguinis, Streptococcus mitis and strains of Candida albicans using broth microdilution method. Phytochemical analysis determined the total phenolic compounds, protein concentration and total of sugars present in the extract. Results: G. Graciliflora demonstrated antifungal activity against the LM 11 and LM 410 clinical isolates of C. albicans (MIC 0.5 mg/mL and 2 mg/mL, respectively). The other microorganisms tested were resistant to the extract. The phytochemical analysis revealed 3% proteins, 13% total sugars and 17% phenolic compounds. Conclusion: G. Graciliflora has antifungal activity against clinical strains of C. albicans and exhibits proteins, sugars and phenolic compounds in its chemical composition.
Descritores: Plantas Medicinais
Técnicas In Vitro
Extratos Vegetais/farmacologia
Anti-Infecciosos
Antibacterianos
-Candida albicans
Testes de Sensibilidade Microbiana
Streptococcus oralis
Streptococcus mitis
Streptococcus salivarius
Antifúngicos
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


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ALVES, Sydney Hartz
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Id: lil-761166
Autor: OLIVEIRA, Daniele Carvalho; LORETO, Érico Silva de; MARIO, Débora Alves Nunes; LOPES, Paulo G. Markus; NEVES, Louise Vignolles; ROCHA, Marta Pires da; SANTURIO, Janio Morais; ALVES, Sydney Hartz.
Título: Sporothrix schenckiicomplex: susceptibilities to combined antifungal agents and characterization of enzymatic profiles / Complexo Sporothrix schenckii: susceptibilidade à combinação de antifúngicos e caracterização dos perfis enzimáticos
Fonte: Rev. Inst. Med. Trop. Säo Paulo;57(4):289-294, July-Aug. 2015. tab.
Idioma: en.
Projeto: CNPq.
Resumo: SUMMARYSporothrix schenckiiwas reclassified as a complex encompassing six cryptic species, which calls for the reassessment of clinical and epidemiological data of these new species. We evaluated the susceptibility of Sporothrix albicans(n = 1) , S. brasiliensis(n = 6) , S. globosa(n = 1), S. mexicana(n = 1) and S. schenckii(n = 36) to terbinafine (TRB) alone and in combination with itraconazole (ITZ), ketoconazole (KTZ), and voriconazole (VRZ) by a checkerboard microdilution method and determined the enzymatic profile of these species with the API-ZYM kit. Most interactions were additive (27.5%, 32.5% and 5%) or indifferent (70%, 50% and 52.5%) for TRB+KTZ, TRB+ITZ and TRB+VRZ, respectively. Antagonisms were observed in 42.5% of isolates for the TRB+VRZ combination. Based on enzymatic profiling, the Sporothrix schenckiistrains were categorized into 14 biotypes. Leucine arylamidase (LA) activity was observed only for S. albicansand S. mexicana. The species S. globosaand S. mexicanawere the only species without β-glucosidase (GS) activity. Our results may contribute to a better understanding of virulence and resistance among species of the genus Sporothrixin further studies.

RESUMOAvaliou-se a susceptibilidade de Sporothrix albicans(n = 1), S. brasiliensis(n = 1), S. globosa(n = 1), S. mexicana(n = 1) e S. schenckii(n = 36) frente à terbinafina (TRB) e a TRB em combinação com itraconazol (ITZ), cetoconazol (KTZ) e voriconazol (VRZ) pelo método de microdiluição ( checkerboard); o perfil enzimático destas espécies foi também avaliado, com base no kit API-ZYM. A maioria das interações foram aditivas (27,5%, 32,5% e 5%) ou indiferentes (70%, 50% e 52,5%) para TRB+KTZ, TRB+ITZ e TRB+VRZ, respectivamente. Antagonismo foi observado em 42,5% dos isolados para a combinação TRB+VRZ. Com base nos perfis enzimáticos, as cepas de Sporothrix schenckiievidenciaram 14 biotipos distintos. A atividade da leucina arilamidase (LA) só foi observada em S. albicanse S. mexicana.As espécies S. globosae S. mexicanaforam as únicas que não evidenciaram atividade da enzima β-glucosidase (GS). Estes resultados poderão contribuir para um melhor entendimento da virulência e resistência entre as espécies do gênero Sporothrixem futuros estudos.
Descritores: Antifúngicos/farmacologia
Sporothrix/efeitos dos fármacos
Sporothrix/enzimologia
-Itraconazol/farmacologia
Cetoconazol/farmacologia
Testes de Sensibilidade Microbiana
Naftalenos/farmacologia
Filogenia
Voriconazol/farmacologia
Limites: Humanos
Animais
Gatos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Zanella, Rosemeire Cobo
Brandileone, Maria Cristina de Cunto
Brandäo, Angela Pires
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Id: lil-763097
Autor: Zanella, Rosemeire Cobo; Brandileone, Maria Cristina de Cunto; Andrade, Ana Lúcia; Ogassavara, Cinthya Terumi; Fiório, Cleiton Eduardo; Brandão, Angela Pires; Almeida, Samanta Cristine Grassi; Lemos, Ana Paula Silva; Gorla, Maria Cecília; Carvalhanas, Telma Regina; Sato, Helena; Liphaus, Bernadete; Nerger, Maria Lígia; Conde, Monica; Ribeiro, Ana Freitas.
Título: Evaluation of Haemophilus influenzae type b carrier status among children 10 years after the introduction of Hib vaccine in Brazil
Fonte: Mem. Inst. Oswaldo Cruz;110(6):755-759, Sept. 2015. tab.
Idioma: en.
Projeto: MCCB; . ALA.
Resumo: The aim of the present study was to assess the prevalence of Haemophilus influenzaetype b (Hib) nasopharyngeal (NP) colonisation among healthy children where Hib vaccination using a 3p+0 dosing schedule has been routinely administered for 10 years with sustained coverage (> 90%). NP swabs were collected from 2,558 children who had received the Hib vaccine, of whom 1,379 were 12-< 24 months (m) old and 1,179 were 48-< 60 m old. Hi strains were identified by molecular methods. Hi carriage prevalence was 45.1% (1,153/2,558) and the prevalence in the 12-< 24 m and 48-< 60 m age groups were 37.5% (517/1,379) and 53.9% (636/1,179), respectively. Hib was identified in 0.6% (16/2,558) of all children in the study, being 0.8% (11/1,379) and 0.4% (5/1,179) among the 12-< 24 m and 48-< 60 m age groups, respectively. The nonencapsulate Hi colonisation was 43% (n = 1,099) and was significantly more frequent at 48-< 60 m of age (51.6%, n = 608) compared with that at 12-< 24 m of age (35.6%, n = 491). The overall resistance rates to ampicillin and chloramphenicol were 16.5% and 3.7%, respectively; the co-resistance was detected in 2.6%. Our findings showed that the Hib carrier rate in healthy children under five years was very low after 10 years of the introduction of the Hib vaccine.
Descritores: Portador Sadio/imunologia
Infecções por Haemophilus/prevenção & controle
Vacinas Anti-Haemophilus/uso terapêutico
Haemophilus influenzae tipo b/imunologia
Nasofaringe/microbiologia
-Resistência a Ampicilina/imunologia
Cápsulas Bacterianas/imunologia
Brasil/epidemiologia
Portador Sadio/microbiologia
Resistência ao Cloranfenicol/imunologia
Estudos Transversais
Infecções por Haemophilus/epidemiologia
Haemophilus influenzae tipo b/classificação
Esquemas de Imunização
Vacinação em Massa
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase
Prevalência
Inquéritos e Questionários
Limites: Humanos
Lactente
Pré-Escolar
Tipo de Publ: Research Support, Non-U.S. Gov't
Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-991369
Autor: Aravena, Carmen; Valencia, Bárbara; Villegas, Andrea; Ortega, Mauricio; Fernández R, Alda; Araya R, Pamela; Saavedra, Aníbal; Del Campo, Rosa.
Título: Caracterización de cepas clínicas y ambientales de Salmonella enterica subsp. enterica serovar Heidelberg aisladas en Chile / Characterization of Salmonella Heidelberg strains isolated in Chile
Fonte: Rev. méd. Chile;147(1):24-33, 2019. tab.
Idioma: es.
Projeto: Dirección de Investigación Universidad de Valparaíso.
Resumo: Background: Salmonella Heidelberg (S. Heidelberg) causes gastroenteritis and sometimes bacteremia and endocarditis. In other countries, this serovar has multidrug resistance including extended-spectrum β-lactamases (ESBLs) and AmpC (β-lactamases (AmpC), associated with the blaCMY-2 gene. In Chile, an outbreak by S. Heidelberg occurred in 2011, the phenotypic and genetic characteristics of Chilean strains are unknown. Aim: To determine the antimicrobial susceptibility, presence of plasmids and virulence factor genes in S. Heidelberg strains isolated in Chile over the period 2006-2011. Material and Methods: In sixty-one S. Heidelberg clinical and environmental strains collected by the Public Health Institute in Chile during 2006-2011, antimicrobial susceptibility, plasmids and virulence factor genes (invA, sifA, pefA, agfA, lpfA and, stkD) were studied. Results: S. Heidelberg had a high susceptibility to sulfamethoxazole-trimethoprim, gentamicin, ceftriaxone, ceftiofur, chloramphenicol, amoxicillin-clavulanic acid and ampicillin. However, 52% had decreased susceptibility to ciprofloxacin and 33% resistance to tetracycline. ESBLs were detected in three strains isolated from blood cultures, environment and human feces. The latter strain was positive for AmpC and blaCMY-2 gene. Fifty three of 61 strains showed one to seven plasmids of 0.8 to approximately 30 kb. Most plasmids were small with sizes between 0.8 and 2 kb. All isolates were positive for all genes except pefA. Conclusions: S. Heidelberg isolated from Chilean samples was susceptible to first-line antimicrobials, except tetracycline and ciprofloxacin. The emergence of strains with ESBLs and AmpC should be a warning. The strains were homogeneous for virulence genes, but heterogeneous in their plasmids.
Descritores: Plasmídeos/isolamento & purificação
Salmonella/isolamento & purificação
Salmonella/efeitos dos fármacos
Antibacterianos/farmacologia
-Valores de Referência
Salmonella/genética
Salmonella/patogenicidade
Fatores de Tempo
Virulência
DNA Bacteriano
Testes de Sensibilidade Microbiana
Chile
Eletroforese em Gel de Campo Pulsado
Farmacorresistência Bacteriana Múltipla
Microbiologia Ambiental
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: lil-589369
Autor: Domínguez Soto, Octavio; Hernández Foronda, John Camilo; Piedrahíta Palacio, Natalia; Saldarriaga Mejía, Nataly; Vanegas Cardona, Diego Mauricio; González Arroyave, Juan Carlos.
Título: Características clínico-epidemiológicas de las infecciones por enterobacter en la clínica cardiovascular de Medellín: agosto de 2004 a agosto de 2006 / Epidemiological profile of enterobacter infections in the clinica cardiovascular Santa Maria in Medellin, August 2004 – August 2006 Colombia
Fonte: Med. U.P.B = Med. UPB;27(2):101-111, jul.-dic. 2008. tab.
Idioma: es.
Resumo: Objetivo: describir las características clínico-epidemiológicas de las infecciones por Enterobacter. Metodología: se revisaron las historias clínicas de 54 pacientes hospitalizados durante agosto de 2004 a agosto de 2006, en la Clínica Cardiovascular de Medellín, con diagnóstico de infección por Enterobacter confirmado por Gram y cultivo. Resultados: 64.8 por ciento de los pacientes del estudio eran hombres con edad media de 48 años. Las especies que se aislaron con mayor frecuencia fueron E. cloacae (75 por ciento) y E. aerogenes (17 por ciento). El tiempo promedio de aislamiento fue de nueve días, luego del ingreso. Las muestras en que se que se aisló la bacteria fueron principalmente orina (42 por ciento) y hemocultivos (34 por ciento); la infección de tracto urinario y la de torrente circulatorio fueron las infecciones que más causó Enterobacter en los pacientes del estudio. La infección fue considerada nosocomial en el 75 por ciento de los casos. Los betalactámicos fueron los antibióticos que con más frecuencia se emplearon como terapia empírica, luego del diagnóstico de infección por Enterobacter y antes del antibiograma, cuyos resultados generaron un cambio en el tratamiento antibiótico en 78 por ciento de los casos. Conclusión: es importante que se tenga en cuenta un manejo empírico de acuerdo a los perfiles de resistencia que esta bacteria está mostrando en la institución.

Objective: to describe the clinical and epidemiological characteristics of infections by Enterobacter. Methods: clinical records of 54 patients who had been hospitalized and had received a confirmed diagnosis of Enterobacterinfection were reviewed. Results: 64.8% of patiens were male, with a mean age of 48. The species which were most commonly isolated included E. cloacae (75%) and E. aerogenes (17%). Mean time for isolation was 9 days after admission to the hospital ward. The samples from which the microorganisms were most commonly isolated were urine (42%) and blood (34%). Urinary tract and bloodstreaminfection were the most commonly caused by Enterobacter. The infection was considered to be hospital-acquired in 75% of cases. Betalactams antibiotics were the empirical choice at the time of treatment, after the infection diagnosis and before the results of antibiograms the treatment was adjusted in 78% of the patients.Conclusion: it is important to keep in mind the local resistance profiles when it comes to treatment of this infection in the institution.
Descritores: Enterobacter
-Bactérias
Testes de Sensibilidade Microbiana
beta-Lactamas
Antibacterianos
Limites: Humanos
Tipo de Publ: Estudo Observacional
Responsável: CO101.1 - BCdeS - Biblioteca Ciencias de la Salud


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Autor: Alippi Adriana M; Reynaldi Francisco J; López Ana C.
Título: Evaluación del método epsilométrico Etest para la determinación de la sensibilidad a tetraciclina en Paenibacillus larvae, agente causal de la loque americana de las abejas / [Evaluation of the Epsilometer (Etest) method for the detection of tetracycline susceptibility in Paenibacillus larvae, the causal agent of American foulbrood disease of honeybees].
Fonte: Rev. argent. microbiol;45(4):257-61, dic. 2013.
Idioma: es.
Resumo: American foulbrood (AFB) is a bacterial disease caused by the spore-forming, grampositive bacterium Paenibacillus larvae, which affects honeybee broods worldwide. The aim of this work was to compare the Epsilometer test (Etest) to the agar dilution method for testing a collection of 22 P. larvae strains to tetracycline by using MYPGP and Iso- Sensitest agars. Results showed that a categorical agreement of 100

was found when using Iso-Sensitest, while a categorical agreement of 86.36

was found (with 3 minor errors) when MYPGP was tested. In conclusion, the Etest could be a rapid and reliable method for testing MIC values of tetracycline in P. larvae only when used in combination with Iso-Sensitest agar. Nevertheless, these results should be confirmed with future studies involving a larger number of isolates.
Descritores: Antibacterianos/farmacologia
Paenibacillus/efeitos dos fármacos
Tetraciclina/farmacologia
-Abelhas/microbiologia
Animais
Infecções por Bactérias Gram-Positivas
Testes de Sensibilidade Microbiana/métodos
Tipo de Publ: Resumo em Inglês
Artigo de Revista
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Responsável: AR5.1 - Centro de Gestión del Conocimiento y las Comunicaciónes



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