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Id: biblio-828194
Autor: Rosca, Irina; Petrovici, Anca Roxana; Brebu, Mihai; Stoica, Irina; Minea, Bogdan; Marangoci, Narcisa.
Título: An original method for producing acetaldehyde and diacetyl by yeast fermentation
Fonte: Braz. j. microbiol;47(4):949-954, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Projeto: \"Program of Excellency in the multidisciplinary doctoral and post-doctoral research of chronic diseases\"; . European Social Fund through the Sectoral Operational Program Human Resources Development; . Romanian National Authority for Scientific Research, CNCS - UEFISCDI.
Resumo: Abstract In this study a natural culture medium that mimics the synthetic yeast peptone glucose medium used for yeast fermentations was designed to screen and select yeasts capable of producing high levels of diacetyl and acetaldehyde. The presence of whey powder and sodium citrate in the medium along with manganese and magnesium sulfate enhanced both biomass and aroma development. A total of 52 yeasts strains were cultivated in two different culture media, namely, yeast peptone glucose medium and yeast acetaldehyde-diacetyl medium. The initial screening of the strains was based on the qualitative reaction of the acetaldehyde with Schiff's reagent (violet color) and diacetyl with Brady's reagent (yellow precipitate). The fermented culture media of 10 yeast strains were subsequently analyzed by gas chromatography to quantify the concentration of acetaldehyde and diacetyl synthesized. Total titratable acidity values indicated that a total titratable acidity of 5.5 °SH, implying culture medium at basic pH, was more favorable for the acetaldehyde biosynthesis using strain D15 (Candida lipolytica; 96.05 mg L-1 acetaldehyde) while a total titratable acidity value of 7 °SH facilitated diacetyl flavor synthesis by strain D38 (Candida globosa; 3.58 mg L-1 diacetyl). Importantly, the results presented here suggest that this can be potentially used in the baking industry.
Descritores: Vírus da Hepatite B/genética
Hepatite C/diagnóstico
Hepatite C/virologia
Hepacivirus/genética
Carga Viral
Hepatite B/diagnóstico
Hepatite B/virologia
-DNA Viral
RNA Viral
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Reação em Cadeia da Polimerase em Tempo Real
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-828211
Autor: Zauli, Danielle Alves Gomes; Menezes, Carla Lisandre Paula de; Oliveira, Cristiane Lommez de; Mateo, Elvis Cristian Cueva; Ferreira, Alessandro Clayton de Souza.
Título: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
Fonte: Braz. j. microbiol;47(4):987-992, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract The quantification of viral nucleic acids in serum by real-time PCR plays an important role in diagnosing hepatitis B virus and hepatitis C virus infection. In this study, we developed an assay using specific primers and probes to quantify hepatitis B virus DNA or hepatitis C virus RNA in serum from infected patients. For standardization and validation of the assay, an international panel of hepatitis B virus/hepatitis C virus and standard plasmids was used. A correlation coefficient of 0.983 and 0.963 for hepatitis B virus and hepatitis C virus, respectively, was obtained based on cycle threshold values and concentrations of DNA or RNA. The standard curve showed a linear relationship from 19 IU/mL to 1.9 × 109 IU/mL of serum, with a coefficient of determination (r2) of 0.99. In sera from patients infected with hepatitis B virus or hepatitis C virus viral loads (19 IU/mL and 1.9 × 109 IU/mL), we quantified viral loads with a detection limit of 1.9 × 102 IU/mL. The real-time quantitative PCR assay developed in this study provides an ideal system for routine diagnosis and confirmation of indeterminate serological results, especially in immunosuppressed patients.
Descritores: Vírus da Hepatite B/genética
Hepatite C/diagnóstico
Hepatite C/virologia
Hepacivirus/genética
Carga Viral
Hepatite B/diagnóstico
Hepatite B/virologia
-DNA Viral
RNA Viral
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Reação em Cadeia da Polimerase em Tempo Real
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-828201
Autor: Riaz, Saba; Bashir, Muhammad Faisal; Haider, Saleem; Rahid, Naeem.
Título: Association of genotypes with viral load and biochemical markers in HCV-infected Sindhi patients
Fonte: Braz. j. microbiol;47(4):980-986, Oct.-Dec. 2016. tab.
Idioma: en.
Resumo: Abstract The presented study had two objectives. The first was to examine distributions of Hepatitis C Virus (HCV) genotypes in Sindh, Pakistan, where HCV is prevalent. The other was to explore clinically relevant relationships between the genotypes, viral load (measured by real-time polymerase chain reaction assays) and biochemical markers. For this, 1471 HCV-infected patients in six cities in Sindh were recruited and sampled. HCV genotype distributions varied among the cities, but genotype 3a was most prevalent, followed by 3b, 1a and 1b (detected in 51.5, 22.7. 9.25 and 3.2% of the cases, respectively). No type-specific sequences were detected in serum samples from 189 (12.8%) of the 1471 patients. Frequencies of low (<200,000 IU/mL serum), intermediate (200,000-600,000 IU/mL serum) and high (>600,000 IU/mL serum) viral loads were respectively 45.4, 16.5 and 38.1% for patients infected with genotype 3, and 16.9, 36.9 and 46.2%, respectively, for patients with other genotypes. Infection with genotype 1a was associated with significantly higher (p < 0.005) alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase titers than infection with genotype 3a. The results will help in the formulation of treatment strategies.
Descritores: Hepatite C/metabolismo
Hepatite C/virologia
Hepacivirus/genética
Carga Viral
Genótipo
-Paquistão/epidemiologia
Biomarcadores
Hepatite C/epidemiologia
Hepacivirus/imunologia
Anticorpos Anti-Hepatite C/imunologia
Anticorpos Anti-Hepatite C/sangue
Limites: Seres Humanos
Masculino
Feminino
Adulto
Meia-Idade
Responsável: BR1.1 - BIREME


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Id: lil-574628
Autor: Mendoza, L. P; Páez, M; Insaurralde, A; Rodríguez, M. I; Castro, A; Kasamatsu, E.
Título: Frecuencia y carga viral relativa del virus del papiloma humano de alto riesgo según el diagnóstico citológico en mujeres paraguayas por captura híbrida II / Frequency and relative viral load of high risk human papilloma virus according to the cytological diagnosis in paraguayan women by hybrid capture II
Fonte: Mem. Inst. Invest. Cienc. Salud (Impr.);6(1):6-13, jun. 2008. tab.
Idioma: es.
Resumo: El cáncer de cuello uterino es el tumor maligno más frecuente en mujeres de Latinoamérica y su agente causal es el virus del papiloma humano (HPV). Recientemente en Paraguay incorporamos el método de captura híbrida II (CH II), el cual detecta 13tipos de HPV de alto riesgo oncogénico (HR-HPV) y proporciona valores relativos de carga viral. El objetivo del estudio fue determinar la carga viral relativa de HR-HPV por CH IIsegún el diagnóstico citológico. Fueron incluidas 566 mujeres (33 + 10 años) atendidas en el Instituto de Investigaciones en Ciencias de la Salud 2006/2009. Fue detectado HRHPVen 43% de las mujeres (241/566), observándose una alta frecuencia del 23% en mujeres con ausencia de lesión intraepitelial (NSIL). Según el diagnóstico citológico, se evidenció una diferencia altamente significativa entre los valores de carga viral relativa(p<0,0001; Kruskal Wallis), observándose un aumento de carga viral relativa de mujeres con NSIL a mujeres con SIL (68 pg/ml en ausencia de SIL; 710 pg/ml para SIL de bajo grado-LSIL y 474 pg/ml para SIL de alto grado-HSIL). No se observó cambio significativo en la carga viral relativa entre LSIL y HSIL (p=0,60; prueba t de Student). Los resultados sugieren que los valores de carga viral relativa proporcionados por CH II pueden ser considerados como un indicador importante en el manejo de mujeres con sospecha de SIL.

Cervical cancer is the most frequent malignant tumor in women of Latin America being human papillomavirus (HPV) the main causative agent. Recently in Paraguay, we incorporated the method of hybrid capture II (CH II) which detects 13 types of HPV ofhigh oncogenic risk (HR-HPV) and provides relative values of viral load. The aim of this study was to determine the frequency and relative viral load of HR-HPV by CH II according to the cytological diagnosis. There were 566 women (33 + 10 years) included in the study that attended the Research Institute in Health Sciences during the period 2006/2009. HR-HPV was detected in 43% of the women (241/566), being observed a high frequency of 23% in women negative to squamous intraepithelial lesions (NSIL). According to the cytological diagnosis, there was a highly significant difference between the values of relative viral load (p <0.0001; Kruskal Wallis), with an increase of relative viral load of women with NSIL to women with SIL (mean values: 68 pg/ml in NSIL; 710 pg/ml in low grade SIL-LSIL and 474 pg/ml in high grade SIL-HSIL). Significant change was not observed in the relative viral load between LSIL and HSIL (p=0.60; Student's t test).The results suggest that the values of relative viral load provided by CH II could be considered an important indicator for managing women with suspicion of SIL.
Descritores: Carga Viral
Neoplasias do Colo do Útero
Responsável: PY3.1 - Biblioteca


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Id: lil-685735
Autor: Cardozo Segovia, F. M; Mendoza Torres, L. P; Páez Acchiardi, G. M; Mongelós Dacunte, P. E; Castro Matto, . AM; Giménez Ortigoza, G.
Título: Detección molecular de infecciones múltiples en mujeres con y sin lesión de cuello uterino positivas para el virus del papiloma humano de alto riesgo, período 2007-2011 / Molecular detection of multiple infections in women with and without cervical lesion positive for high risk human papillomavirus, period 2007-2011
Fonte: Mem. Inst. Invest. Cienc. Salud (Impr.);10(2):5-12, dic. 2012. tab.
Idioma: es.
Resumo: El cáncer de cuello uterino, causado por el virus del papiloma humano (HPV), constituye el tercer tipo de cáncer más común en mujeres en el mundo. El objetivo del presente trabajo fue determinar por reacción en cadena de la polimerasa seguida de hibridación reversa, la frecuencia de infecciones múltiples en mujeres positivas para HPV de alto riesgo oncogénico por captura híbrida II, según severidad de la lesión escamosa intraepitelial (SIL), carga viral relativa y edad, en el periodo 2007-2011. Este estudio de corte transverso incluyó 123 mujeres que acudieron al Instituto de Investigaciones en Ciencias de la Salud, 18 sin lesión (NSIL), 78 con SIL de bajo grado (LSIL) y 27 con SIL de alto grado (HSIL). Se detectaron infecciones múltiples en 37/123 mujeres (30,1%). Las mujeres con NSIL presentaron menor frecuencia de infecciones múltiples (5,6%) que las mujeres con SIL (34,3%) (p=0,03). No se observó aumento de frecuencia de infecciones múltiples con la severidad de la lesión, presentando las mujeres con LSIL un 34,6% y con HSIL, un 33,3%. No fue observada diferencia significativa entre la carga viral relativa de infecciones simples y múltiples, lo cual sugiere que en la infección múltiple podría ser solo uno de los tipos de HPV el que se esté replicando, causando una infección productiva. Además, se observó una alta frecuencia de infecciones múltiples (75%) en mujeres menores a 30 años. Finalmente los resultados sugieren que las infecciones múltiples podrían orientar la presencia de SIL, pero no el grado de severidad de la lesión cervical.
Descritores: Doenças Sexualmente Transmissíveis
PAPILLOMAVIRUS HUMANO ABELSON MURINE LEUKEMIA VIRUS
PAPILLOMAVIRUS HUMANO ABNORMALITIES, MULTIPLE
PAPILLOMAVIRUS HUMANO 1ABDOMINAL NEOPLASMS
-Carga Viral
Responsável: PY3.1 - Biblioteca


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Id: lil-644779
Autor: Souza, Anderson Jambeiro de; Cruz, Simone Seixas da; Davoglio, Rosane Silvia; Gomes Filho, Isaac S; Passos, Johelle Santana; Damasceno, Murilo C. M; Figueiredo, Ana Claudia Moraes Godoy.
Título: Fatores associados à presença de HIV: resultados preliminares no município de Juazeiro, Bahia / Factors associated with presence of HIV: preliminary results in the municipality of Juazeiro, Bahia / Factores asociados a la presencia del HIV: resultados preliminares en el municipio de Juazeiro, Bahia
Fonte: Rev. baiana saúde pública;36(1), jan-mar. 2012. tab, graf.
Idioma: pt.
Descritores: Síndrome de Imunodeficiência Adquirida
HIV
Carga Viral
Fatores Imunológicos
Responsável: BR15.1 - Biblioteca de Ciências Biomédicas


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Id: lil-423257
Autor: Tirado-Gómez, Laura Leticia; Mohar-Betancourt, Alejandro; López-Cervantes, Malaquías; García-Carrancá, Alejandro; Franco-Marina, Francisco; Borges, Guilherme.
Título: Factores de riesgo de cáncer cervicouterino invasor en mujeres mexicanas / Risk factors in invasive cervical cancer among Mexican women
Fonte: Salud pública Méx;47(5):342-350, sept.-oct. 2005. tab.
Idioma: es.
Projeto: CONACyT. 26198-M.
Resumo: OBJETIVOS: Evaluar la asociación entre cáncer cervicouterino (CaCu) invasor y el virus del papiloma humano de alto riesgo (VPH-AR) (carga viral/tipo 16), así como con factores ginecológicos y socioeconómicos. MATERIAL Y MÉTODOS: Estudio de casos y controles pareado individualmente (215 casos con CaCu invasor y 420 controles). La población de estudio se reclutó entre los años 2000 y 2001. Se evaluaron variables tradicionalmente asociadas con CaCu (ginecológicas y socioeconómicas) y dos variables asociadas con la presencia de VPH (carga viral y el tipo 16). La presencia de VPH-AR se determinó mediante Captura de Híbridos II. La carga viral se midió a través de unidades relativas de luz y picogramos por ml (1 RLU=1 pg/ml), divididas en cuatro categorías: negativa (<1 pg/ml), baja carga viral (1-49 pg/ml), carga viral intermedia (50-499 pg/ml) y alta carga viral (>499 pg/ml). El análisis estimó razones de momios (RM) ajustadas a través de modelos de regresión logística condicionada. RESULTADOS: La presencia de VPH-AR incrementa en 78 veces la probabilidad de presentar CaCu invasor; cuando el VPH es tipo 16, el incremento es mayor (RM= 429.7) comparado con otros tipos (RM=64.1). Se observó una tendencia importante en la RM al elevarse la carga viral (RM=46.6 carga baja; RM=250.7 intermedia y RM=612.9 alta). Finalmente, los factores demográficos y obstétricos conocidos, incrementaron la probabilidad de CaCu invasor. No se observó asociación entre CaCu invasor y tabaquismo en la población de estudio. CONCLUSIONES: Este estudio contribuye a la identificación de las mujeres con alto riesgo de desarrollar CaCu invasor, entre las pacientes infectadas con VPH-AR. Por otra parte, confirma la importancia de la infección de VPH-AR y refleja la carga viral del VPH-AR como cofactor y posible promotor en el desarrollo de la enfermedad. Por último, este biomarcador puede contribuir a mejorar la prevención y la detección temprana de esta enfermedad.
Descritores: Papillomaviridae/isolamento & purificação
Infecções por Papillomavirus/complicações
Neoplasias do Colo do Útero/complicações
Neoplasias do Colo do Útero/virologia
Carga Viral
-Estudos de Casos e Controles
México
Invasividade Neoplásica
Fatores de Risco
Neoplasias do Colo do Útero/patologia
Limites: Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Seres Humanos
Meia-Idade
Responsável: BR1.1 - BIREME


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Lemos, Marcílio Figueiredo
Moreira, Regina Celia
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Id: lil-772614
Autor: Santos, Ana Paula de Torres; Levi, José Eduardo; Lemos, Marcilio Figueiredo; Calux, Samira Julien; Oba, Isabel Takano; Moreira, Regina Célia.
Título: An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA
Fonte: Mem. Inst. Oswaldo Cruz;111(2):134-140, Feb. 2016. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: This study aimed to standardise an in-house real-time polymerase chain reaction (rtPCR) to allow quantification of hepatitis B virus (HBV) DNA in serum or plasma samples, and to compare this method with two commercial assays, the Cobas Amplicor HBV monitor and the Cobas AmpliPrep/Cobas TaqMan HBV test. Samples from 397 patients from the state of São Paulo were analysed by all three methods. Fifty-two samples were from patients who were human immunodeficiency virus and hepatitis C virus positive, but HBV negative. Genotypes were characterised, and the viral load was measure in each sample. The in-house rtPCR showed an excellent success rate compared with commercial tests; inter-assay and intra-assay coefficients correlated with commercial tests (r = 0.96 and r = 0.913, p < 0.001) and the in-house test showed no genotype-dependent differences in detection and quantification rates. The in-house assay tested in this study could be used for screening and quantifying HBV DNA in order to monitor patients during therapy.
Descritores: DNA Viral/isolamento & purificação
Técnicas de Genotipagem/normas
Vírus da Hepatite B/isolamento & purificação
Hepatite B Crônica/diagnóstico
Técnicas de Diagnóstico Molecular
Reação em Cadeia da Polimerase em Tempo Real/normas
-Primers do DNA/normas
Estudos de Avaliação como Assunto
Genótipo
Soropositividade para HIV/sangue
Soropositividade para HIV/diagnóstico
Vírus da Hepatite B/genética
Hepatite B Crônica/sangue
Hepatite C/sangue
Hepatite C/diagnóstico
Invenções/normas
Técnicas de Diagnóstico Molecular/instrumentação
Técnicas de Diagnóstico Molecular/métodos
Sensibilidade e Especificidade
Carga Viral
Limites: Seres Humanos
Masculino
Feminino
Lactente
Pré-Escolar
Criança
Adolescente
Adulto
Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: biblio-1020077
Autor: Strottmann, Daisy Maria; Zanluca, Camila; Mosimann, Ana Luiza Pamplona; Koishi, Andrea C; Auwerter, Nathalia Cavalheiro; Faoro, Helisson; Cataneo, Allan Henrique Depieri; Kuczera, Diogo; Wowk, Pryscilla Fanini; Bordignon, Juliano; Duarte dos Santos, Claudia Nunes.
Título: Genetic and biological characterisation of Zika virus isolates from different Brazilian regions
Fonte: Mem. Inst. Oswaldo Cruz;114:e190150, 2019. tab, graf.
Idioma: en.
Projeto: FIOCRUZ; . BNDES; . CNPq; . CAPES; . Fundação Araucária; . CNDS; . JB; . CNPq; . DMS; . CZ; . ALPM.
Resumo: BACKGROUND Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. OBJECTIVES The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 Brazilian epidemics. METHODS The ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. FINDINGS Eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the well-established ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. MAIN CONCLUSIONS Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells.
Descritores: Aedes/virologia
Zika virus/genética
Infecção pelo Zika virus/virologia
Camundongos Endogâmicos BALB C
-Filogenia
Cultura de Vírus
Replicação Viral
Células Vero
Brasil
Cercopithecus aethiops
Carga Viral
Limites: Seres Humanos
Animais
Responsável: BR1.1 - BIREME


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Machado, Alcyone Artioli
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Id: biblio-842770
Autor: Lourenço, Alan Grupioni; Ribeiro, Ana Elisa Rodrigues Alves; Nakao, Cristiano; Motta, Ana Carolina Fragoso; Antonio, Luana Grupioni Lourenço; Machado, Alcyone Artioli; Komesu, Marilena Chinali.
Título: Oral Candida spp carriage and periodontal diseases in HIV-infected patients in Ribeirão Preto, Brazil
Fonte: Rev. Inst. Med. Trop. Säo Paulo;59:e29, 2017. tab, graf.
Idioma: en.
Projeto: National Counsel of Technological and Scientific Development.
Resumo: ABSTRACT The majority of HIV-infected patients develop Candida spp-associated clinical oral lesions. Studies have shown that asymptomatic oral colonization of Candida spp may lead to oral lesions or become a source of disseminated infections. The aim of this study was to verify the effects of periodontal conditions on Candida spp prevalence and Candida spp carriage in the oral cavity of HIV-infected patients compared to non-infected patients. Twenty-five patients not infected with HIV and 48 HIV-infected patients were classified according to periodontal conditions as being periodontal healthy or with periodontal disease. Candida spp carriage and classification were performed in oral rinse samples. Viral load and CD4+ T lymphocyte (CD4+L) counts were performed in blood samples from HIV-infected patients. No differences in Candida spp prevalence related to HIV status or periodontal condition were detected. However, Candida spp carriage was increased in periodontally affected HIV-infected patients when compared to periodontally healthy HIV-infected patients (p= 0.04). Periodontally healthy HIV-infected patients presented Candida spp carriage in similar levels as healthy or periodontally affected non-HIV-infected patients. Candida spp carriage was correlated with CD4+L counting in HIV-infected patients. We concluded that periodontal disease is associated with increased Candida spp carriage in HIV-infected patients and may be a predisposing factor to clinical manifestations of candidiasis.
Descritores: Doenças Periodontais/microbiologia
Candida/classificação
Candidíase Bucal/microbiologia
Infecções Oportunistas Relacionadas com a AIDS/microbiologia
-Doenças Periodontais/epidemiologia
Brasil/epidemiologia
Candida/isolamento & purificação
Candidíase Bucal/epidemiologia
Portador Sadio
Infecções Oportunistas Relacionadas com a AIDS/epidemiologia
Contagem de Linfócito CD4
Carga Viral
Boca/microbiologia
Limites: Seres Humanos
Masculino
Feminino
Adulto
Responsável: BR1.1 - BIREME



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