Base de dados : LILACS
Pesquisa : E01.370.225.875.970 [Categoria DeCS]
Referências encontradas : 65 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 7 ir para página                  

  1 / 65 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1020077
Autor: Strottmann, Daisy Maria; Zanluca, Camila; Mosimann, Ana Luiza Pamplona; Koishi, Andrea C; Auwerter, Nathalia Cavalheiro; Faoro, Helisson; Cataneo, Allan Henrique Depieri; Kuczera, Diogo; Wowk, Pryscilla Fanini; Bordignon, Juliano; Duarte dos Santos, Claudia Nunes.
Título: Genetic and biological characterisation of Zika virus isolates from different Brazilian regions
Fonte: Mem. Inst. Oswaldo Cruz;114:e190150, 2019. tab, graf.
Idioma: en.
Projeto: FIOCRUZ; . BNDES; . CNPq; . CAPES; . Fundação Araucária; . CNDS; . JB; . CNPq; . DMS; . CZ; . ALPM.
Resumo: BACKGROUND Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. OBJECTIVES The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 Brazilian epidemics. METHODS The ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. FINDINGS Eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the well-established ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. MAIN CONCLUSIONS Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells.
Descritores: Aedes/virologia
Zika virus/genética
Infecção pelo Zika virus/virologia
Camundongos Endogâmicos BALB C
-Filogenia
Cultura de Vírus
Replicação Viral
Células Vero
Brasil
Cercopithecus aethiops
Carga Viral
Limites: Seres Humanos
Animais
Responsável: BR1.1 - BIREME


  2 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-984761
Autor: Cataneo, Allan Henrique Depieri; Kuczera, Diogo; Mosimann, Ana Luiza Pamplona; Silva, Emanuele Guimarães; Ferreira, Álvaro Gil Araújo; Marques, João Trindade; Wowk, Pryscilla Fanini; Santos, Claudia Nunes Duarte dos; Bordignon, Juliano.
Título: Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus
Fonte: Mem. Inst. Oswaldo Cruz;114:e180432, 2019. tab, graf.
Idioma: en.
Projeto: Ministério da Saúde; . Fundação Araucária; . Fundação Araucária; . SESA-PR; . CNPq; . MS-Decit; . PPSUS; . JTM; . CNDS; . JB; . CNPq; . AHDC; . DK; . CNDS; . JB.
Resumo: BACKGROUND The Zika virus (ZIKV) epidemics that affected South America in 2016 raised several research questions and prompted an increase in studies in the field. The transient and low viraemia observed in the course of ZIKV infection is a challenge for viral isolation from patient serum, which leads to many laboratories around the world sharing viral strains for their studies. C6/36 cells derived from Aedes albopictus larvae are commonly used for arbovirus isolation from clinical samples and for the preparation of viral stocks. OBJECTIVES Here, we report the contamination of two widely used ZIKV strains by Brevidensovirus, here designated as mosquito densovirus (MDV). METHODS Molecular and immunological techniques were used to analyse the MDV contamination of ZIKV stocks. Also, virus passages in mammalian cell line and infecting susceptible mice were used to MDV clearance from ZIKV stocks. FINDINGS MDV contamination was confirmed by molecular and immunological techniques and likely originated from C6/36 cultures commonly used to grow viral stocks. We applied two protocols that successfully eliminated MDV contamination from ZIKV stocks, and these protocols can be widely applied in the field. As MDV does not infect vertebrate cells, we performed serial passages of contaminated stocks using a mammalian cell line and infecting susceptible mice prior to re-isolating ZIKV from the animals' blood serum. MDV elimination was confirmed with immunostaining, polymerase chain reaction (PCR), and analysis of the mosquitoes that were allowed to feed on the infected mice. MAIN CONCLUSIONS Since the putative impact of viral contaminants in ZIKV strains generally used for research purposes is unknown, researchers working in the field must be aware of potential contaminants and test viral stocks to certify sample purity.
Descritores: Cultura de Vírus
Bancos de Espécimes Biológicos
Zika virus
-DNA Viral
Imunofluorescência
Densovirus/genética
Camundongos
Limites: Seres Humanos
Animais
Responsável: BR1.1 - BIREME


  3 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-999132
Autor: Macero Méndez, Reina María; Galindo Banegas, Thelmo.
Título: FRECUENCIA DE ESCHERICHIA COLI BETALACTAMASA DE ESPECTRO EXTENDIDO (BLEE), EN PACIENTES CON INFECCION DE VÍAS URINARIAS. HOSPITAL JOSÉ CARRASCO ARTEAGA / Frequency of Escherichia Coli Beta lactamase extended spectrum (ESBL), in patients with urinary tract infection. Hopital José Carrasco Arteaga
Fonte: Rev. Fac. Cienc. Méd. Univ. Cuenca;35(1):74-78, Abril 2017. tab.
Idioma: es.
Resumo: OBJETIVOS: Determinar la Frecuencia de Eschericia coli Beta Lactamasa de Espectro Extendido en Infecciones de Vías Urinarias, en el hospital José Carrasco Arteaga 2012-2013. MÉTODOLOGÍA: Observacional de tipo descriptivo prospectivo transversal. La población estudiada estuvo conformada por 605 muestras de urocultivos de pacien-tes de las áreas de: consulta externa, emergencia, hos-pitalización y cuidados intensivos. Se efectuó la prueba del método de confirmación apropiado, basado en la inhibición de la enzima ß-Lactamasas de confirmación productora de ß-Lactamasa según las normas Stan-dard Institute Clinical Laboratory. Se realizó el análisis estadístico descriptivo para comprensión e interpreta-ción de datos. RESULTADOS: Se cultivó 605 muestras, se reportó E. coli en 455 muestras de las cuales 82 correspondieron a la cepa productora de ß-Lactamasas de Expectro Ex-tendido un 18%. Según las variables consideradas, de acuerdo al sexo las mujeres representaron el mayor por-centaje con un 87,8%, el grupo etario con mayor repor-te fue el de 51-60 años con el 20,7%, seguido del grupo de 61-70 con el 17,1%, según la procedencia, el área urbana representó 69,5%, de acuerdo a los servicios en consulta externa se reportó 37,8% y en emergencia el 34,1%. CONCLUSIÓN: en el hospital José Carrasco Arteaga en el periodo septiembre 2012-enero 2013 se reportó una prevalencia del 18% de E. coli productora de ß-Lacata-masa en muestras de urocultivo de pacientes de los ser-vicios de consulta externa, emergencia, hospitalización y cuidados intensivos. Las mujeres fueron las más afec-tadas, según procedencia el mayor porcentaje fue del área urbana, el grupo de adultos representó el mayor porcentaje y la consulta externa fue el servicio con mayor frecuencia. (Impacto de los resultados

OBJECTIVES: To determine the frequency of Escherichia coli extended spectrum lactamasa in urinary tract in-fections, at José Carrasco Arteaga Hospital, 2012-2013. METHODOLOGY: It is an observational descriptive cross-sectional study. The population consisted of 605 samples of urine cultures of patients from the areas of: external consultation, emergency, hospitalization and intensive care. The appropriate confirmatory method was tested, it was based on the inhibition of ß-Lacta-masa-producing confirmation enzyme according to the Standard Institute Clinical Laboratory. A descriptive sta-tistical analysis was performed for data comprehension and interpretation. RESULTS: A total of 605 samples were cultured, E. coli was reported in 455 samples, and only 82 corresponded to the extended Expectrum ß-lactamase producing strain with 18%. According to the variables considered, regar-ding sex, women represented the highest percentage with 87.8%, the highest age group was 51-60 years with 20.7%, followed by the group of 61-70 with 17.1%, de-pending on the source, the urban area accounted for 69.5%, according to the services in external consultation 37.8% were reported, and 34.1% were emergency ones. CONCLUSION: A prevalence of 18% of E. coli producing ß-Lactamasa was reported at the José Carrasco Artea-ga Hospital in the period September 2012-January 2013 in urine samples of patients from external consultation, emergency, hospitalization and Intensive care servi-ces. The women were the most affected, according to provenance, the highest percentage was of the urban area, the group of adults represented the highest per-centage and the external consultation service was the most frequent. (Impact of results).
Descritores: Sistema Urinário
beta-Lactamases
Escherichia coli
-Cultura de Vírus
Análise Estatística
Infecção
Limites: Seres Humanos
Masculino
Feminino
Meia-Idade
Tipo de Publ: Estudo Observacional
Responsável: EC3.1 - Biblioteca


  4 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Cuba
Texto completo
Id: lil-752966
Autor: Burguet Lago, Nancy; Trimiño Romero, José A; Sierra Prado, Nelson.
Título: Evaluación del cultivo liofilizado de Candida albicans utilizado en esquemas de certificaciones de calidad / Evaluation of the lyophilized culture of Candida albicans used in quality certification schemes
Fonte: Rev. cuba. hig. epidemiol;52(3):321-329, set.-dic. 2014.
Idioma: es.
Resumo: Introducción: el laboratorio de control microbiológico de la UEB Laboratorios Liorad dispone de una colección de cultivos microbianos para la conservación de microorganismos, donde se encuentra depositada la levadura Candida albicans que se emplea en esquemas de certificaciones de calidad establecidos para la evaluación de ensayos como: promoción de crecimiento de los medios de cultivos, validación de técnicas microbiológicas, entre otros. Objetivo: evaluar los resultados de la conservación de esta cepa por el método de liofilización durante un periodo de ocho años. Métodos: para el crecimiento de la cepa se utilizó el medio de cultivo Caldo Saboraud y variantes de sustancias lioprotectoras puras como: (leche descremada al 20 por ciento, glicerol 20 por ciento, sacarosa al 10 por ciento y peptona 5 por ciento) así como la mezcla de lioprotectores (leche 10 por ciento, sacarosa 5 por ciento, glicerol 5 por ciento). Se evaluó viabilidad, pureza y estabilidad genética de esta cepa durante el tiempo objeto de estudio. Resultados: las características propias de la especie estudiada se mantuvieron inalterables con un elevado grado de pureza en todas las variantes estudiadas. En cuanto a la supervivencia, cuando se emplearon las sustancias lioprotectoras puras se evidenció una marcada disminución de la viabilidad. No así al emplear la mezcla de lioprotectores que mantuvo niveles de viabilidad por encima del límite establecido durante todo el tiempo objeto de estudio. Conclusiones: los valores obtenidos en cuanto a la supervivencia de este microorganismo permiten inferir que para la conservación por largos periodos de tiempo la variante donde se empleó mezclas de lioprotectores resultó una buena opción para la conservación de C. albicans(AU)

Introduction: the microbiological control laboratory at the Basic Enterprise Unit Liorad Laboratories stores a collection of microbial cultures for the preservation of microorganisms, including the Candida albicans yeast used in the quality certification schemes established for the evaluation of assays such as fostering of culture medium growth and validation of microbiological techniques, among others. Objective: evaluate the results obtained in the preservation of this strain by the lyophilization method during a period of eight years. Methods: for strain growth, use was made of Saboraud broth culture medium as well as variants of pure lyoprotective substances such as 20 percent skimmed milk, 20 percent glycerol, 10 percent saccharose and 5 percent peptone, and the mixture of lyoprotectors (10 percent milk, 5 percent saccharose, 5 percent glycerol). An evaluation was conducted of the viability, purity and genetic stability of the strain during the study period. Results: characteristics typical of the study species remained unchanged with a high degree of purity in all the variants examined. As to survival, a marked reduction in viability was observed when pure lyoprotective substances were used, but not with the mixture of lyoprotectors, in which case viability levels exceeded the established limit during the entire study period. Conclusions: the survival values obtained for this microorganism indicate that preservation for long periods with mixtures of lyoprotectors was a good option for the preservation of C. albicans(AU)
Descritores: Candida albicans/fisiologia
ISO 9000/métodos
Liofilização/métodos
-Cultura de Vírus/estatística & dados numéricos
Técnicas Microbiológicas/métodos
Limites: Seres Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


  5 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Cuba
Texto completo
Texto completo
Id: biblio-960377
Autor: Pacheco López, Pablo; Estévez Ramos, Rafael Antonio; Basset Machado, Ihosvanny; Barco González, Ana Isabel; Sánchez Soto, Juan Manuel.
Título: Repercusión de los dispositivos móviles en la atención de enfermería a usuarios en estado crítico / Repercussion of mobile devices on nursing care for users in critical state
Fonte: Rev. cuba. enferm;32(4):0-0, oct.-dic. 2016. ilus, tab.
Idioma: es.
Resumo: Introducción: actualmente en los hospitales de México, especialmente en las áreas de cuidados críticos, se ha incrementado el uso de dispositivos móviles de comunicación, repercutiendo en el cuidado del paciente; esto pudiera representar no solamente un distractor, sino una fuente portadora de gérmenes. Objetivo: evaluar la repercusión de los dispositivos móviles en la atención de enfermería a usuarios en estado crítico. Métodos: estudio descriptivo, trasversal; donde fueron medidos los tiempos de interrupción del cuidado de enfermería en el uso de dispositivos móviles de comunicación; se describió la exposición de estos artefactos con los equipos biomédicos por medio de una guía observacional, además se tomó muestra de los dispositivos móviles para su cultivo en agar nutritivo. Resultados: el 75,00 por ciento de los enfermeros estudiados hacían uso de los dispositivos móviles dentro de su jornada laboral; el 68,00 por ciento hizo uso de algún dispositivo móvil mientras realizaba alguna actividad con el paciente; el 64,00 por ciento tenía contacto con equipo biomédico; el 100,00 por ciento no se lavaba las manos antes y después de usarlos; en el 100,00 por ciento de las muestras tomadas y cultivadas hubo crecimiento Unidades Formadoras de Colonias a las 48 horas. Conclusiones: los dispositivos móviles son distractores, adictivos y cuentan con carga bacteriológica, esto afecta en la atención directa al paciente, su uso aún no está regulado; por esta razón sería importante considerar limitar el uso en las unidades de cuidados críticos, esto ayudara a brindar una mejor atención viéndose reflejado en la seguridad del paciente(AU)

Introduction: In Mexico hospitals today, especially in critical care areas, the use of mobile devices of communication has increased, which has had a repercussion on the care for the patient; this could represent not only a distracting aspect, but a germ-bearing source. Objective: Assess the repercussion of mobile devices on nursing care for user in critical state. Methods: cross-sectional, descriptive study in which we measured the interruption times for nursing care in the use of mobile devices of communication; we described the exposition of this artifacts with biomedical equipment by means of an observational guide, we also took sample of mobile devices for their culture in a nutrient agar. Results: 75.00 percentof the studied nurses used mobile devices within their working day; 68.00 percent used any mobile device while doing any activity with the patient; 64.00 percent had contact with biomedical equipment; 100.00 percent did not wash their hands before or after using them; in the 100.00 percent of the samples taken and cultured there were colonies growing after 48 hours. Conclusions: Mobile devices are distracting, addictive and have bacteriologic charge, which affects the direct care for the patient, their use is not regulated; therefore, it would be important to consider limiting their use in critical care units, which will help provide better attention reflected on the patient's safety(AU)
Descritores: Cultura de Vírus/métodos
Telefone Celular/estatística & dados numéricos
Enfermagem de Cuidados Críticos/estatística & dados numéricos
-Epidemiologia Descritiva
Estudos Transversais
Limites: Seres Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


  6 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-884378
Autor: Ponce, Carlos Felipe; Madrid, Wilmer Alejandro; Pineda, Iván José.
Título: Agentes bacterianos en la sepsis neonatal. cuidados intensivos neonatales, Hospital Mario Catarino Rivas / Bacterial agents in neonatal sepsis. Neonatal Intensive Care unit Mario Catarino Rivas National Hospital
Fonte: Acta pediátr. hondu;6(2):479-486, oct,-2015. tab., graf..
Idioma: es.
Resumo: Antecedentes: Las infecciones nosocomiales son aquellas que se adquieren y se mani estan luego de 48 horas de hospitalización Objetivo: Determinar los gérmenes aislados por cultivos de los recién nacidos diagnosticados como sepsis nosocomial en la unidad de cuidados intensivos neonatales (UCIN), Hospital Nacio- nal Mario Catarino Rivas (HNMCR), en los meses de julio a septiembre del 2015. Pacientes y métodos: Estudio transversal, de los 443 pacientes ingresados a UCIN, 221 neonatos que desarrollaron infección posterior a 48 horas de internamiento. La información se obtuvo del expediente clínico y se procesó en el software estadístico Epi Info 3.02 Resulta- dos: De los cultivos obtenidos; (165) 75% resultaron positivos para algún germen especí- co. Los gérmenes aislados fueron; Pseudomo- na spp 71 (43%) y Pseudomona aeruginosa 58 (35%), haciendo un total de 78% de sepsis nosocomial por Pseudomona. Conclusión: La sepsis intrahospitalaria es un problema frecuente en UCIN, por lo tanto es necesario el cumplimiento de las normas de vigilancia y control de este tipo de infecciones...(AU)
Descritores: Cuidados Críticos
Infecção Hospitalar/mortalidade
Sepse Neonatal/diagnóstico
-Cultura de Vírus/métodos
Limites: Seres Humanos
Recém-Nascido
Responsável: HN1.1 - Biblioteca Médica Nacional


  7 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Venezuela
Texto completo
Id: lil-749973
Autor: Celis, Argelia; Moros, Zoila; Gerder, Marlene; Pagano, Francesca; Vizzi, Esmeralda; Liprandi, Ferdinando.
Título: Selección de variantes de virus dengue sensibles a la heparina en células BHK-21 / Selection of heparin-sensitive dengue virus variants in BHK-21 cells
Fonte: Invest. clín;55(2):155-167, jun. 2014. ilus, tab.
Idioma: es.
Resumo: Estudios previos han demostrado que la adaptación de diversos virus a crecer en líneas celulares de vertebrados, conduce a la selección de variantes virales que unen al heparán sulfato (HS) con alta afinidad. En el presente trabajo se determinó la susceptibilidad de cepas del virus dengue (DENV) a la heparina hipersulfatada un análogo al HS, después de pases seriados en células BHK-21. A aislados de campo de los cuatro serotipos de DENV, se les realizaron ocho pases seriados en células BHK-21. La adaptación de los DENV al cultivo celular seleccionó variantes virales con una aumentada capacidad replicativa en células BHK-21 y una incrementada susceptibilidad a la heparina, en relación a las respectivas cepas no adaptadas, obteniéndose una inhibición de la infectividad más significativa en DENV-2 y DENV-4. Las cepas de DENV adaptadas presentaron cambios en la secuencia de aminoácidos de la proteína de envoltura (E), en particular una substitución K204R para DENV-1, N67K para DENV-2, K308R y V452A para DENV-3 y E327G para DENV-4. Estas sustituciones implicaron ganancia de residuos básicos que incrementaron la carga neta positiva de la proteína. Los resultados sugieren, que la adaptación de cepas de DENV a células BHK-21 selecciona variantes virales sensibles a la heparina y que la efectividad de este compuesto varía dependiendo de la cepa viral. Además sugieren que el HS puede jugar un papel importante en la infectividad de las cepas de DENV adaptadas al cultivo celular, a diferencia de los aislados de DENV no adaptados.

Several studies have shown that adaptation of various viruses to grow in certain cell lines of vertebrates, leads to the selection of virus variants that bind heparan sulfate (HS) with high affinity. In this study we investigated the susceptibility of strains of dengue virus (DENV) to oversulfated heparin an analogue of HS after passages in BHK-21 cells. Field isolates of the four serotypes of DENV with a limited number of passes in mosquito cells C6/36HT were serially passaged eight times in BHK-21 cells. The adaptation of the DENV to the cell culture selected viral variants with an increased replicative capacity in BHK-21 cells and an increased susceptibility to heparin compared with the original not adapted strains, with a more significant inhibition of the infectivity in DENV-2 and DENV-4.The E protein of the adapted strains showed changes in the amino acid sequence, particularly at the position K204R to DENV-1, N67K to DENV-2, K308R and V452A for DENV-3 and E327G to DENV-4. These substitutions implicated a gain of basic residues that increased the net positive charge of the protein. These results suggest that adaptation of DENV strains to BHK-21 cells implies changes in the envelope protein, changes associated to the protein reactivity with heparin, the inhibitory effectiveness of this compound varying depending on the viral strain. In addition, these results suggest that the HS can play an important role in the infectivity of the DENV strains adapted to vertebrate cell culture, but not in the infectivity of non-adapted DENV isolates.
Descritores: Vírus da Dengue/efeitos dos fármacos
Heparina/farmacologia
Seleção Genética
Proteínas do Envelope Viral/genética
-Aedes/citologia
Linhagem Celular
Cercopithecus aethiops
Vírus da Dengue/crescimento & desenvolvimento
Rim/citologia
Mesocricetus
Modelos Moleculares
Mutação
Mutação de Sentido Incorreto
Ligação Proteica
Conformação Proteica
RNA Viral/genética
Análise de Sequência de RNA
Células Vero
Ensaio de Placa Viral
Cultura de Vírus
Replicação Viral
Proteínas do Envelope Viral/química
Proteínas do Envelope Viral/fisiologia
Limites: Animais
Cricetinae
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


  8 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Roehe, Paulo Michel
Texto completo
Id: lil-748263
Autor: Kruger, Ernesto Renato; Penha, Tania Regina; Stoffelo, Daura Regina Eira; Roehe, Paulo Michel; Ribeiro, Magda Costa; Soccol, Vanete Thomaz.
Título: Bovine Herpesvirus 4 in Parana State, Brazil: case report, viral isolation, and molecular identification
Fonte: Braz. j. microbiol;46(1):279-283, 05/2015. graf.
Idioma: en.
Resumo: Bovine Herpesvirus 4 (BoHV-4) is a member of Gammaherpesvirinae sub-family and belongs to genus Rhadinovirus. This virus has been associated with different clinical manifestations and research activity has put forward a strong correlation among virus infection, postpartum metritis, and abortion. The goal of this work was to characterize a virus strain isolate from a cow’s uterine outflow. From swabs drawn of uterine secretion, a virus strain was isolated and characterized by its cytopathology, morphology, and molecular biology approaches. In culture there was CPE development, characterized mainly by long strands with several small balloons along them, radiated from infected cells. Electron microscopy analysis revealed virus particles that had icosahedrical capsid symmetry surrounded by a loose envelope, typical of a herpesvirus. A 2,571 bp PCR product after HindIII digestion generated four fragments, whose base pair composition were 403, 420, 535, and 1,125 bp. Restriction enzymes HindIII and BamHI generated the expected diagnostic bands as well as a 2,350 bp hypermolar fragment as a result of BamHI treatment to demonstrate that agent was a bovine herpesvirus 4, appertaining to DN-599 group.
Descritores: Doenças dos Bovinos/virologia
Infecções por Herpesviridae/veterinária
/classificação
HERPESVIRUS ABBREVIATIONS AS TOPIC, BOVINE/classificação
/isolamento & purificação
HERPESVIRUS ABBREVIATIONS AS TOPIC, BOVINE/isolamento & purificação
Infecções Tumorais por Vírus/veterinária
-Brasil
Efeito Citopatogênico Viral
DNA Viral/genética
DNA Viral/metabolismo
Exsudatos e Transudatos/virologia
Infecções por Herpesviridae/virologia
/genética
HERPESVIRUS ABBREVIATIONS AS TOPIC, BOVINE/genética
Microscopia Eletrônica de Transmissão
Polimorfismo de Fragmento de Restrição
Infecções Tumorais por Vírus/virologia
Útero/patologia
Útero/virologia
Cultura de Vírus
Vírion/ultraestrutura
Limites: Animais
Bovinos
Feminino
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


  9 / 65 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-741314
Autor: Silva Jr., J.V.J.; Arenhart, S.; Santos, H.F.; Almeida-Queiroz, S.R.; Silva, A.N.M.R.; Trevisol, I.M.; Bertani, G.R.; Gil, L.H.V.G..
Título: Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast
Fonte: Braz. j. microbiol;45(4):1555-1563, Oct.-Dec. 2014. ilus, graf, tab.
Idioma: en.
Resumo: The Infectious Bursal Disease Virus (IBDV) causes immunosuppression in young chickens. Advances in molecular virology and vaccines for IBDV have been achieved by viral reverse genetics (VRG). VRG for IBDV has undergone changes over time, however all strategies used to generate particles of IBDV involves multiple rounds of amplification and need of in vitro ligation and restriction sites. The aim of this research was to build the world's first VRG for IBDV by yeast-based homologous recombination; a more efficient, robust and simple process than cloning by in vitro ligation. The wild type IBDV (Wt-IBDV-Br) was isolated in Brazil and had its genome cloned in pJG-CMV-HDR vector by yeast-based homologous recombination. The clones were transfected into chicken embryo fibroblasts and the recovered virus (IC-IBDV-Br) showed genetic stability and similar phenotype to Wt-IBDV-Br, which were observed by nucleotide sequence, focus size/morphology and replication kinetics, respectively. Thus, IBDV reverse genetics by yeast-based homologous recombination provides tools to IBDV understanding and vaccines/viral vectors development.
Descritores: Recombinação Homóloga
Vírus da Doença Infecciosa da Bursa/genética
Genética Reversa/métodos
-Brasil
Células Cultivadas
Fibroblastos/virologia
Vetores Genéticos
Instabilidade Genômica
Vírus da Doença Infecciosa da Bursa/isolamento & purificação
Vírus da Doença Infecciosa da Bursa/fisiologia
Saccharomyces cerevisiae/genética
Transfecção
Cultura de Vírus
Replicação Viral
Limites: Animais
Embrião de Galinha
Responsável: BR1.1 - BIREME


  10 / 65 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Venezuela
Texto completo
Id: lil-740332
Autor: Odreman-Macchioli, María; Vielma, Silvana; Atchley, Daniel; Comach, Guillermo; Ramirez, Alvaro; Pérez, Saberio; Téllez, Luis; Quintero, Beatriz; Hernández, Erick; Muñoz, Maritza; Mendoza, José.
Título: Analysis of real time PCR amplification efficiencies from three genomic region of dengue virus / Análisis de las eficiencias de amplificación por PCR en tiempo real de tres regiones genómicas del virus dengue
Fonte: Invest. clín;54(1):5-19, mar. 2013. tab.
Idioma: en.
Resumo: Early diagnosis of dengue virus (DENV) infection represents a key factor in preventing clinical complications attributed to the disease. The aim of this study was to evaluate the amplification efficiencies of an in-house quantitative real time-PCR (qPCR) assay of DENV, using the non-structural conserved genomic region protein-5 (NS5) versus two genomic regions usually employed for virus detection, the capsid/pre-membrane region (C-prM) and the 3'-noncoding region (3'NC). One-hundred sixty seven acute phase serum samples from febrile patients were used for validation purposes. Results showed that the three genomic regions had similar amplification profiles and correlation coefficients (0.987-0.999). When isolated viruses were used, the NS5 region had the highest qPCR efficiencies for the four serotypes (98-100%). Amplification from acute serum samples showed that 41.1% (67/167) were positive for the universal assay by at least two of the selected genomic regions. The agreement rates between NS5/C-prM and NS5/3'NC regions were 56.7% and 97%, respectively. Amplification concordance values between C-prM/NS5 and NS5/3'NC regions showed a weak (k= 0.109; CI 95%) and a moderate (k= 0.489; CI 95%) efficiencies in amplification, respectively. Serotyping assay using a singleplex NS5-TaqMan® format was much more sensitive than the C-prM/SYBR Green® I protocol (76%). External evaluation showed a high sensitivity (100%), specificity (78%) and high agreement between the assays. According to the results, the NS5 genomic region provides the best genomic region for optimal detection and typification of DENV in clinical samples.

El diagnóstico precoz de la infección por el virus dengue (DENV) constituye un elemento clave para la prevención de las complicaciones clínicas propias de la enfermedad. El objetivo del estudio fue evaluar la detección de DENV mediante un ensayo cuantitativo de PCR-tiempo real (qPCR), desarrollado localmente, utilizando la región no-estructural-5 (NS5), versus dos regiones tradicionalmente empleadas para la detección del virus, la región cápside/pre-membrana (C-prM), y la región noncodificante-3' (3'NC). Se recolectaron 167 muestras de suero de pacientes en fase aguda de la enfermedad. Las tres regiones génicas tuvieron perfiles de amplificación/coeficientes de correlación similares (0,987-0,999). Sin embargo, la región NS5 tuvo la eficiencia de amplificación más elevada para los cuatro serotipos (98-100%). Durante el proceso de validación, 41,1% (67/167) de las muestras de suero resultaron positivas para DENV al menos por dos de las regiones genómicas empleadas. Los valores de concordancia entre las regiones NS5/C-prM y NS5/3'NC fueron de 56,7% y 97%, respectivamente. La concordancia fue débil entre las regiones NS5/C-prM (k= 0,109; CI 95%), sin embargo, fue moderada entre las regiones NS5/3'NC (k= 0,489; CI 95%). El ensayo de tipificación uniplex en formato NS5/TaqMan® mostró alta sensibilidad (100%) que el protocolo C-prM/SYBRGreen®-I (76%). La validación externa del ensayo mostró una alta sensibilidad (100%), especificidad (78%) y acuerdo alto entre los ensayos utilizados. De acuerdo a los resultados obtenidos, la región NS5 ofrece la mayor opción para la detección y serotipificación del DENV en muestras clínicas.
Descritores: /genética
ABATTOIRS' UNTRANSLATED REGIONS/genética
Proteínas do Capsídeo/genética
Vírus da Dengue/genética
Dengue/virologia
Genoma Viral
Reação em Cadeia da Polimerase em Tempo Real
RNA Viral/análise
Proteínas não Estruturais Virais/genética
-Anticorpos Antivirais/sangue
Vírus da Dengue/classificação
Vírus da Dengue/imunologia
Vírus da Dengue/isolamento & purificação
Dengue/sangue
Diagnóstico Precoce
Ensaio de Imunoadsorção Enzimática
Imunoglobulina M/sangue
Compostos Orgânicos
Reprodutibilidade dos Testes
Reação em Cadeia da Polimerase em Tempo Real/métodos
Sensibilidade e Especificidade
Sorotipagem
Taq Polimerase
Cultura de Vírus
Limites: Seres Humanos
Tipo de Publ: Estudo Comparativo
Estudos de Avaliação
Research Support, Non-U.S. Gov't
Estudos de Validação
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha



página 1 de 7 ir para página                  
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde