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Id: biblio-1049751
Autor: Munhoz, Tania.
Título: Aplicação da microscopia confocal in vivo como método diagnóstico complementar ao exame de dermatoscopia digital: um estudo longitudinal retrospectivo / Application of in vivo confocal microscopy as a complementary diagnostic method to digital dermatoscopy examination: a longitudinal retrospective study.
Fonte: São Paulo; s.n; 2019. 116 p. ilust, tabelas.
Idioma: pt.
Tese: Apresentada a Fundação Antônio Prudente para obtenção do grau de Doutor.
Resumo: Introdução: O melanoma cutâneo é uma neoplasia que apresenta comportamento agressivo quando em estágio de evolução avançado, de forma que o sucesso no tratamento depende do diagnóstico precoce. A utilização da microscopia confocal in vivo (MC) como técnica complementar à dermatoscopia digital tem se mostrado útil para aumentar a acurácia diagnóstica dessa neoplasia. Semelhante à dermatoscopia, a MC revela detalhes morfológicos da arquitetura tecidual no plano paralelo à pele e, além disso, fornece imagens instantâneas com alta magnificação e resolução celular. Objetivo: Determinar como a utilização da microscopia confocal in vivo como técnica complementar à dermatoscopia digital pode melhorar a acurácia no diagnóstico do melanoma e reduzir o número de excisões cirúrgicas desnecessárias em um hospital referência de oncologia cutânea. Demonstrar por meio do cálculo do Número necessário para tratar (NNT), o impacto da associação dos métodos de imagem na rotina de um hospital referência em oncologia cutânea. Material e método: Trata-se de um estudo, retrospectivo observacional a ser realizado no Departamento de Oncologia Cutânea do A.C.Camargo Cancer Center. Foram incluídas 209 lesões melanocíticas duvidosas, divididas em dois grupos de análise: as lesões que sofreram modificações no seguimento dermatoscópico, sugestivas de melanoma, encaminhadas para excisão cirúrgica, após realização da MC (Grupo de lesões excisadas) e outro grupo em que os achados de benignidade da MC indicaram o seguimento da lesão (Grupo de lesões em seguimento). Todos os casos foram analisados em 3 etapas distintas: dermatoscopia isolada da lesão a ser analisada antes do exame de MC, utilizando-se o método de Análise de Padrão; dermatoscopia digital comparativa: análise das mudanças que levaram a indicação da MC; análise da Microscopia confocal, utilizando protocolo do Departamento. Para o cáculo do NNT da dermatoscopia de seguimento, foram analisadas mais 300 lesões melanocíticas de pacientes que estavam em acompanhamento digital. Resultados: Por meio da regressão logística simples e múltipla, foram criados dois nomogramas de análise para predizer a chance de uma dada lesão ser um melanoma. Posteriormente, de forma inédita, foi calculado valores de NNT em 3 cenários distintos. Os valores obtidos foram de 7,89 para dermatoscopia digital de seguimento, 5,8 para MC e um NNT hipotético de 3,09 quando aplicado aos nomogramas criados, aplicados na amostra. Conclusão: O presente estudo comprova a importância da MC como método de avaliação complementar no seguimento dos nevos melanocíticos em pacientes com alto risco para câncer de pele, aumentando a detecção do melanoma no seu estágio inicial, por meio da criação de dois nomogramas e a criação de uma aplicativo para a sua aplicabilidade.Comprovou-se a redução significativa do NNT quando associado o exame de microscopia confocal à dermatoscopia digital na rotina ambulatorial de seguimento dos pacientes de alto risco num hospital oncológico de referência (AU)

Introduction: Cutaneous melanoma is a neoplasm that presents aggressive behavior when in advanced stage of evolution, so success in treatment depends on early diagnosis. The use of in vivo confocal microscopy (CM) as a complementary technique to digital dermatoscopy has been shown to be useful to increase the diagnostic accuracy of this neoplasm. Similar to dermoscopy, CM reveals morphological details of tissue architecture in the plane parallel to the skin and, in addition, provides instant images with high magnification and cellular resolution. Objective: To determine how the use of in vivo confocal microscopy as a complementary technique to digital dermatoscopy can improve the accuracy of melanoma diagnosis and reduce the number of unnecessary surgical excisions in a cutaneous oncology referral hospital. Demonstrate by calculating the Number Needed to Treat (NNT) the impact of combining imaging methods on the routine of a referral cutaneous oncology hospital. Material and method: This is a retrospective observational study to be performed at the Department of Cutaneous Oncology, Hospital A.C.Camargo Cancer Center. We included 209 dubious melanocytic lesions divided into two groups of analysis: lesions that underwent changes in the dermoscopic follow-up, suggestive of melanoma, which were referred for surgical excision after MC (Excised lesions group) and another group in which the findings of benignity of the MC indicated follow-up of the lesion (Group of injuries in follow-up). All cases were analyzed in 3 distinct stages: isolated dermatoscopy of the lesion to be analyzed before the MC examination, using the Pattern Analysis method; Comparative digital dermatoscopy: analysis of changes that led to the indication of MC, analysis of confocal microscopy, using MC protocol of the Department. For the NNT calculation of the follow up dermatoscopy, it was analysed more than 300 melanocytic lesions from patients that were under digital follow up. Results: Through simple and multiple logistic regression, two analysis nomograms were created to predict the chance of a given lesion being a melanoma. Subsequently, unpublished, NNT values were calculated in 3 different scenarios. The values obtained were 7.89 for follow-up digital dermatoscopy, 5.8 for MC and a hypothetical NNT of 3.09 when applying the nomograms applied to the sample. Conclusion: The current study proves the importance of the MC as complementar evaluation method in the follow up of the melanocytic nevi in patients with high risk for skin cancer, increasing the melanoma detection in its early stage. Through the creation of two new nomograms and its applicability by the creation of an app, it was proven a significative reduction of the NNT when the confocal microscopy examination is associate to the digital dermoscopic in the ambulatory routine of high risk patients follow up in an oncologic reference hospital (AU)
Descritores: Estudos Retrospectivos
Microscopia Confocal
Dermoscopia
Números Necessários para Tratar
Estudo Observacional
Histologia
Melanoma
Nevo
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Responsável: BR30.1 - Biblioteca
BR30.1


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Id: biblio-990107
Autor: Trindade, Juliana Rocha Mol; Figueiredo, Viviane Rossi; Pêgo-Fernandes, Paulo Manuel; Terra, Ricardo Mingarini.
Título: Probe-based confocal laser endomicroscopy of the airways: physiological and pathological characteristics of preneoplastic and neoplastic lesions / Endomicroscopia confocal a laser das vias aéreas: características normais e patológicas de lesões pré-neoplásicas e neoplásicas
Fonte: J. bras. pneumol;45(1):e20180103, 2019. graf.
Idioma: en.
Descritores: Lesões Pré-Cancerosas/patologia
Lesões Pré-Cancerosas/diagnóstico por imagem
Neoplasias do Sistema Respiratório/patologia
Neoplasias do Sistema Respiratório/diagnóstico por imagem
Microscopia Confocal/métodos
Endoscopia/métodos
-Alvéolos Pulmonares/patologia
Alvéolos Pulmonares/diagnóstico por imagem
Estudos Prospectivos
Reprodutibilidade dos Testes
Elastina
Limites: Humanos
Tipo de Publ: Carta
Responsável: BR1.1 - BIREME


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Id: biblio-1038274
Autor: Teixeira, Danilo Augusto; Rezze, Gisele Gargantini; Pinhal, Maria Aparecida Silva; Paschoal, Francisco Macedo.
Título: Reflectance confocal microscopy as a tool for screening surgical margins of basal cell carcinoma
Fonte: An. bras. dermatol;93(4):601-604, July-Aug. 2018. tab, graf.
Idioma: en.
Resumo: Abstract: Surgical excision of basal cell carcinoma with minimum margins requires serial assessment of layers by frozen histopathology in the case of Mohs micrographic surgery. Evaluation of presurgical tumor margins by in vivo reflectance confocal microscopy is a potential alternative. We selected 12 basal cell carcinoma lesions that were analyzed by confocal microscopy to define margins. The lesions were excised by Mohs surgery. Six tumors showed negative margins in the first phase of Mohs micrographic surgery. We concluded that reflectance confocal microscopy can be useful in the preoperative definition of basal cell carcinoma margins.
Descritores: Neoplasias Cutâneas/cirurgia
Carcinoma Basocelular/cirurgia
Cirurgia de Mohs/métodos
-Neoplasias Cutâneas/patologia
Carcinoma Basocelular/patologia
Microscopia Confocal
Margens de Excisão
Limites: Humanos
Feminino
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: biblio-983740
Autor: Pasquali, Paola; Gonzalez, Salvador; Fortuño, Angeles; Freites-Martinez, Azael.
Título: In-vivo assessment of a case of cutaneous sarcoidosis using reflectance confocal microscopy
Fonte: An. bras. dermatol;94(1):93-95, Jan.-Feb. 2019. graf.
Idioma: en.
Resumo: Abstract: Reflectance confocal microscopy (RCM) is a noninvasive imaging technique that allows visualization of the epidermis and papillary dermis with cellular-level resolution. Granulomatous reactions such as sarcoidosis could be assessed using RCM. The identification of bright beaded-like structures that could correspond to reticulin fibers overlying granulomas, in association with dermoscopy, may be a very useful approach in the diagnosis of sarcoidosis and for the differentiation of this granulomatous entity with superficial cutaneous metastasis.
Descritores: Sarcoidose/diagnóstico por imagem
Dermatopatias/diagnóstico por imagem
Microscopia Confocal/métodos
-Sarcoidose/patologia
Dermatopatias/patologia
Biópsia
Dermoscopia/métodos
Granuloma/patologia
Granuloma/diagnóstico por imagem
Limites: Humanos
Feminino
Idoso
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: biblio-887016
Autor: Veasey, John Verrinder; Avila, Ricardo Bertozzi de; Ferreira, Marcus Antônio Maia de Olivas; Lazzarini, Rosana.
Título: Reflectance confocal microscopy of tinea nigra: comparing images with dermoscopy and mycological examination results
Fonte: An. bras. dermatol;92(4):568-569, July-Aug. 2017. graf.
Idioma: en.
Resumo: Abstract: Tinea nigra is a superficial mycosis whose diagnosis is confirmed by isolating the infectious agent Hortae werneckii through mycological examinations. In vivo reflectance confocal microscopy, initially used in melanocytic dermatosis, has been used with skin infectious diseases to identify the parasite at the cellular level. We report, for the first time in the scientific literature, the use of reflectance confocal microscopy in a case of tinea nigra and compare its findings to dermoscopy and mycological examination results.
Descritores: Tinha/diagnóstico por imagem
Microscopia Confocal/métodos
-Tinha/microbiologia
Dermoscopia/métodos
Fungos/isolamento & purificação
Limites: Humanos
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: lil-797977
Autor: Lei, Lei; Shao, Meiying; Yang, Yan; Mao, Mengying; Yang, Yingming; Hu, Tao.
Título: Exopolysaccharide dispelled by calcium hydroxide with volatile vehicles related to bactericidal effect for root canal medication
Fonte: J. appl. oral sci;24(5):487-495, Sept.-Oct. 2016. graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: ABSTRACT Objective: Enterococcus faecalis is the dominant microbial species responsible for persistent apical periodontitis with ability to deeply penetrate into the dentin. Exopolysaccharides (EPS) contribute to the pathogenicity and antibiotic resistance of E. faecalis. Our aim was to investigate the antimicrobial activity of calcium hydroxide (CH), camphorated parachlorophenol (CMCP), and chlorhexidine (CHX) against E. faecalis in dentinal tubules. Material and Methods: Decoronated single-canal human teeth and semicylindrical dentin blocks were incubated with E. faecalis for 3 weeks. Samples were randomly assigned to six medication groups for 1 week (n=10 per group): CH + 40% glycerin-water solution (1:1, wt/vol); CMCP; 2% CHX; CH + CMCP (1:1, wt/vol); CH + CMCP (2:3, wt/vol); and saline. Bacterial samples were collected and assayed for colony-forming units. After dentin blocks were split longitudinally, confocal laser scanning microscopy was used to assess the proportion of viable bacteria and EPS production in dentin. Results: CMCP exhibited the best antimicrobial activity, while CH was the least sensitive against E. faecalis (p<0.05). CHX showed similar antimicrobial properties to CH + CMCP (1:1, wt/vol) (p>0.05). CH combined with CMCP inhibited EPS synthesis by E. faecalis, which sensitized biofilms to antibacterial substances. Moreover, increasing concentrations of CMCP decreased EPS matrix formation, which effectively sensitized biofilms to disinfection agents. Conclusion: The EPS matrix dispelled by CH paste with CMCP may be related to its bactericidal effect; the visualization and analysis of EPS formation and microbial colonization in dentin may be a useful approach to verify medicaments for antimicrobial therapy.
Descritores: Polissacarídeos Bacterianos/química
Irrigantes do Canal Radicular/farmacologia
Veículos Farmacêuticos/farmacologia
Hidróxido de Cálcio/farmacologia
Enterococcus faecalis/efeitos dos fármacos
Dentina/efeitos dos fármacos
Antibacterianos/farmacologia
-Fatores de Tempo
Cânfora/farmacologia
Contagem de Colônia Microbiana
Clorofenóis/farmacologia
Reprodutibilidade dos Testes
Estatísticas não Paramétricas
Microscopia Confocal
Biofilmes/efeitos dos fármacos
Cavidade Pulpar/efeitos dos fármacos
Cavidade Pulpar/microbiologia
Dentina/microbiologia
Combinação de Medicamentos
Viabilidade Microbiana/efeitos dos fármacos
Limites: Humanos
Adolescente
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


  7 / 221 LILACS  
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Id: lil-792589
Autor: WANG, Linda; BIM JÚNIOR, Odair; LOPES, Adolfo Coelho de Oliveira; FRANCISCONI-DOS-RIOS, Luciana Fávaro; MAENOSONO, Rafael Massunari; D’ALPINO, Paulo Henrique Perlatti; HONÓRIO, Heitor Marques; ATTA, Maria Teresa.
Título: Water interaction and bond strength to dentin of dye-labelled adhesive as a function of the addition of rhodamine B
Fonte: J. appl. oral sci;24(4):317-324, July-Aug. 2016. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . São Paulo Research Foundation.
Resumo: ABSTRACT Objective This study investigated the effect of the fluorescent dye rhodamine B (RB) for interfacial micromorphology analysis of dental composite restorations on water sorption/solubility (WS/WSL) and microtensile bond strength to dentin (µTBS) of a 3-step total etch and a 2-step self-etch adhesive system. Material and Methods The adhesives Adper Scotchbond Multi-Purpose (MP) and Clearfil SE Bond (SE) were mixed with 0.1 mg/mL of RB. For the WS/WSL tests, cured resin disks (5.0 mm in diameter x 0.8 mm thick) were prepared and assigned into four groups (n=10): MP, MP-RB, SE, and SE-RB. For µTBS assessment, extracted human third molars (n=40) had the flat occlusal dentin prepared and assigned into the same experimental groups (n=10). After the bonding and restoration procedures, specimens were sectioned in rectangular beams, stored in water and tested after seven days or after 12 months. The failure mode of fractured specimens was qualitatively evaluated under optical microscope (x40). Data from WS/WSL and µTBS were assessed by one-way and three-way ANOVA, respectively, and Tukey’s test (α=5%). Results RB increased the WSL of MP and SE. On the other hand, WS of both MP and SE was not affected by the addition of RB. No significance in µTBS between MP and MP-RB for seven days or one year was observed, whereas for SE a decrease in the µTBS means occurred in both storage times. Conclusions RB should be incorporated into non-simplified DBSs with caution, as it can interfere with their physical-mechanical properties, leading to a possible misinterpretation of bonded interface.
Descritores: Rodaminas/química
Água/química
Adesivos Dentinários/química
Dentina/efeitos dos fármacos
Corantes Fluorescentes/química
-Solubilidade
Propriedades de Superfície
Resistência à Tração
Fatores de Tempo
Teste de Materiais
Reprodutibilidade dos Testes
Colagem Dentária/métodos
Microscopia Confocal
Resinas Compostas/química
Cimentos de Resina/química
Falha de Restauração Dentária
Limites: Humanos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  8 / 221 LILACS  
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Id: biblio-1015826
Autor: Vásquez-Ponce, Felipe; Higuera-Llantén, Sebastián; Pavlov, María Soledad; Ramírez-Orellana, Ramón; Marshall, Sergio H; Olivares-Pacheco, Jorge.
Título: Alginate overproduction and biofilm formation by psychrotolerant Pseudomonas mandelii depend on temperature in Antarctic marine sediments
Fonte: Electron. j. biotechnol;28:27-34, July. 2017. tab, ilus, graf.
Idioma: en.
Projeto: Dirección de Investigación, Pontificia Universidad Católica de Valparaíso.
Resumo: Background: In recent years, Antarctica has become a key source of biotechnological resources. Native microorganisms have developed a wide range of survival strategies to adapt to the harsh Antarctic environment, including the formation of biofilms. Alginate is the principal component of the exopolysaccharide matrix in biofilms produced by Pseudomonas, and this component is highly demanded for the production of a wide variety of commercial products. There is a constant search for efficient alginate-producing organisms. Results: In this study, a novel strain of Pseudomonas mandelii isolated from Antarctica was characterized and found to overproduce alginate compared with other good alginate producers such as Pseudomonas aeruginosa and Pseudomonas fluorescens. Alginate production and expression levels of the alginate operon were highest at 4°C. It is probable that this alginate-overproducing phenotype was the result of downregulated MucA, an anti-sigma factor of AlgU. Conclusion: Because biofilm formation is an efficient bacterial strategy to overcome stressful conditions, alginate overproduction might represent the best solution for the successful adaptation of P. mandelii to the extreme temperatures of the Antarctic. Through additional research, it is possible that this novel P. mandelii strain could become an additional source for biotechnological alginate production.
Descritores: Pseudomonas/metabolismo
Alginatos/metabolismo
-Polissacarídeos Bacterianos/metabolismo
Pseudomonas/crescimento & desenvolvimento
Pseudomonas/genética
Adaptação Biológica
Temperatura Baixa
Microscopia Confocal
Biofilmes
Feófitas
Tipagem de Sequências Multilocus
Reação em Cadeia da Polimerase em Tempo Real
Regiões Antárticas
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-893657
Autor: Figueiredo, Viviane Maria Gonçalves de; Pereira, Sarina Maciel Braga; Bressiani, Eduardo; Valera, Márcia Carneiro; Bottino, Marco Antônio; Zhang, Yu; Melo, Renata Marques de.
Título: Effects of porcelain thickness on the flexural strength and crack propagation in a bilayered zirconia system
Fonte: J. appl. oral sci;25(5):566-574, Sept.-Oct. 2017. tab, graf.
Idioma: en.
Projeto: US National Institute of Dental and Craniofacial Research.
Resumo: Abstract Objective: This study evaluated the influence of porcelain (VM9, VITA Zahnfabrik, Germany) thickness on the flexural strength and crack propagation in bilayered zirconia systems (YZ, VITA Zahnfabrik, Germany). Material and Methods: Thirty zirconia bars (20.0x4.0x1.0 mm) and six zirconia blocks (12.0x7.5x1.2 mm) were prepared and veneered with porcelain with different thickness: 1 mm, 2 mm, or 3 mm. The bars of each experimental group (n=10) were subjected to four-point flexural strength testing. In each ceramic block, a Vickers indentation was created under a load of 10 kgf for 10 seconds, for the propagation of cracks. Results: The results of flexural strength were evaluated by One-way ANOVA and Tukey's test, with a significance level of 5%. The factor "thickness of the porcelain" was statistically significant (p=0.001) and the l-mm group presented the highest values of flexural strength. The cracks were predominant among the bending specimens with 1 and 2 mm of porcelain, and catastrophic failures were found in 50% of 3-mm-thick porcelain. After the indentation of blocks, the most severe defects were observed in blocks with 3-mm-thick porcelain. Conclusion: The smallest (1 mm) thickness of porcelain on the zirconia infrastructure presented higher values of flexural strength. Better resistance to defect propagation was observed near the porcelain/ zirconia interface for all groups. Higher flexural strength was found for a thinner porcelain layer in a bilayered zirconia system. The damage caused by a Vickers indentation near and far the interface with the zirconia shows that the stress profiles are different.
Descritores: Zircônio/química
Cerâmica/química
Porcelana Dentária/química
-Valores de Referência
Propriedades de Superfície
Resistência à Tração
Fatores de Tempo
Teste de Materiais
Reprodutibilidade dos Testes
Análise de Variância
Maleabilidade
Microscopia Confocal
Falha de Restauração Dentária
Análise do Estresse Dentário
Testes de Dureza
Temperatura Alta
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-889469
Autor: Zancan, Rafaela Fernandes; Canali, Lyz Cristina Furquim; Tartari, Talita; Andrade, Flaviana Bombarda de; Vivan, Rodrigo Ricci; Duarte, Marco Antonio Hungaro.
Título: Do different strains of E. faecalis have the same behavior towards intracanal medications in in vitro research?
Fonte: Braz. oral res. (Online);32:e46, 2018. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Abstract: The aim of this study was to evaluate the antimicrobial action of different endodontic pastes against Enterococcus faecalis ATCC 29212, isolated from the urinary tract, and compare the action with E. faecalis ATCC 4083, isolated from the root canal. For this purpose, dentin blocks were infected for 21 days with both bacteria at different time-intervals to ensure there would be no cross contamination. After this period, blocks were immersed in the test medications for 7 days, according to the following groups: CH/S, CH/P, CH/CMCP, CH/CHX, CH/DAP and TAP. Images of the samples were captured with a confocal microscope and the percentage of live cells was computed by means of the Bioimage program. The ATCC 29212 strain was shown to be more resistant to CH/SS, Calen, CH/DAP, and TAP than the ATCC 4083 strain. The antimicrobial action of the medications against each strain were divergent concerning the order of susceptibility. The authors concluded that the strains behaved in a different manner: in general, those extracted from the urinary tract were more resistant to the tested medications. Therefore, when E. faecalis must be used for in vitro research in endodontics, we suggest the use of ATCC 4083 strain to obtain results that are closer to the clinical reality.
Descritores: Hidróxido de Cálcio/farmacologia
Clorexidina/farmacologia
Enterococcus faecalis/efeitos dos fármacos
Biofilmes/efeitos dos fármacos
Cavidade Pulpar/microbiologia
Antibacterianos/farmacologia
-Microscopia Eletrônica de Varredura
Enterococcus faecalis/classificação
Enterococcus faecalis/ultraestrutura
Microscopia Confocal
Biofilmes/classificação
Cavidade Pulpar/ultraestrutura
Limites: Animais
Bovinos
Responsável: BR1.1 - BIREME



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