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Id: biblio-1056928
Autor: Santos, Heidy C. dos; Vieira, Dielson S; Yamamoto, Sandra M; Costa, Mateus M; Sá, Maria C. A; Silva, Eva M. S; Silva, Tania M. S.
Título: Antimicrobial activity of propolis extract fractions against Staphylococcus spp. isolated from goat mastitis / Atividade antimicrobiana de frações do extrato de própolis frente à Staphylococcus spp. isolados de mastite caprina
Fonte: Pesqui. vet. bras;39(12):954-960, Dec. 2019. tab, graf, ilus.
Idioma: en.
Resumo: The indiscriminate use of antibiotics in the treatment of caprine mastitis causes the appearance of resistant microorganisms, besides leaving residues in milk, putting at risk to human health. In this way, propolis is an alternative in the treatment of diseases because it has antimicrobial activity, mainly because of the presence of flavonoids in its composition. The aim of this study was to evaluate the antimicrobial potential of propolis to Staphylococcus spp. Isolated from cases of goat mastitis and qualify the crude ethanoic extract by high performance liquid chromatography (HPLC). In this study, the minimum bactericidal concentration values of propolis extracts in ethanol, ethyl acetate and hexane showed that the best concentrations capable of promoting the highest mortality of the isolates of Staphylococcus spp. from mastitis in goats, were 6250, 3125 and 1562.5µg/mL, respectively. By the microplate adherence test, it was found that 20.78% isolates were not able to form biofilm, 14.70% were classified as moderate and 64.70% were weak and none as a strong biofilm producer. Propolis in its different diluents was able to affect the formation of biofilm and showed a pronounced marked antimicrobial activity against Staphylococcus spp. strains and may be indicated for use in in vivo studies.(AU)

O uso indiscriminado de antibióticos no tratamento de mastite caprina leva ao desenvolvimento de micro-organismos resistentes que poderão estar presentes em alimentos, colocando em risco a saúde humana. Dessa forma, a própolis surge como uma alternativa para o tratamento de doenças por possuir uma ação antimicrobiana, principalmente pela presença de flavonoides em sua composição. O objetivo desse estudo foi avaliar o potencial antimicrobiano da própolis frente à Staphylococcus spp. isolados de casos de mastite caprina e qualificar o extrato etanoico bruto por cromatografia líquida de alta eficiência (CLAE-DAD). Neste estudo, os valores de concentração bactericida mínima (CBM) dos extratos de própolis em álcool etílico, acetato de etila e hexano nos isolados foram de 6250, 3125 e 1562,5µg/mL, respectivamente. Pelo teste de aderência à microplacas, observou-se que 20,78% dos microorganismos, não foram capazes de formar biofilme, 14,70% foram classificados como moderados, 64,70% em fracos e nenhum como forte produtor de biofilme. A própolis em seus diferentes diluentes foi capaz de afetar a formação de biofilme e apresentou significativa atividade antimicrobiana frente a cepas de Staphylococcus spp., podendo ser indicada para utilização em estudos "in vivo".(AU)
Descritores: Própole/uso terapêutico
Infecções Estafilocócicas/terapia
Infecções Estafilocócicas/veterinária
Staphylococcus/isolamento & purificação
Cabras/microbiologia
Apiterapia/veterinária
Mastite/terapia
Mastite/veterinária
Limites: Animais
Feminino
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-783794
Autor: Medeiros, Vanessa de Fátima Lima Paiva; Azevedo, Ítalo Medeiros; Rêgo, Amália Cínthia Meneses; Egito, Eryvaldo Sócrates Tabosa do; Araújo-Filho, Irami; Medeiros, Aldo Cunha.
Título: Antibacterial properties and healing effects of Melipona scutellaris honey in MRSA-infected wounds of rats
Fonte: Acta cir. bras;31(5):327-332, May 2016. tab.
Idioma: en.
Resumo: ABSTRACT PURPOSE : To investigate the antimicrobial, immunological and healing effects of Melipona scutellaris honey on infected wounds of rat skin. METHODS: Twenty four Wistar rats were distributed in four groups (6-each). The uninfected skin wounds of group I rats were treated daily with saline for 7 days. Uninfected wounds (group II) rats were treated with honey. In group III (treated with saline) and group IV (treated with honey) wounds were inoculated with MRSA ATTC43300. The first bacterial culture was performed 24 hours later. In the 7th day new culture was done, and wound biopsies were used for cytokines dosage and histopathology. RESULTS: In group I and III rats the CFU/g count of S. aureus in wounds was zero. In group II rats the CFU/g counts in the wound tissue were significantly higher than in wounds of group IV rats. The density histopathological parameters and the expression of TNF-α, IL1-β, Il-6 were significantly higher on wounds of group IV then in the other groups. CONCLUSION: Honey of Melipona scutellaris was effective in the management of infected wounds, by significant bacterial growth inhibition, enhancement of cytokine expression, and positively influenced the wound repair.
Descritores: Infecções Estafilocócicas/terapia
Cicatrização
Staphylococcus aureus Resistente à Meticilina
Apiterapia
Mel
-Infecções Estafilocócicas/microbiologia
Infecção dos Ferimentos/microbiologia
Infecção dos Ferimentos/terapia
Distribuição Aleatória
Colágeno/análise
Interleucina-6/análise
Fator de Necrose Tumoral alfa/análise
Ratos Wistar
Modelos Animais
Interleucina-1beta/análise
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Carga Bacteriana
Fibroblastos
Leucócitos
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Mello, J. R. B
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Id: biblio-1011330
Autor: Peter, C. M; Waller, S. B; Picoli, T; Osório, L. G; Zani, J. L; Meireles, M. C. A; Faria, R. O; Mello, J. R. B; Hubner, S. O; Lima, M; Fischer, G.
Título: Chemical and cytotoxic analyses of three varieties of Brazilian propolis (green propolis, jataí propolis and brown propolis) and its anti-Sporothrix brasiliensis in vitro activity / [Análises químicas e citotóxicas de três variedades de própolis brasileiras (própolis verde, própolis jataí e própolis marrom) e sua atividade in vitro anti-Sporothrix brasiliensis]
Fonte: Arq. bras. med. vet. zootec. (Online);71(3):819-827, May-June 2019. tab, graf.
Idioma: en.
Resumo: In this study, we described the antifungal activity of three Brazilian propolis extracts: brown, green and from jataí bees against Sporothrix brasiliensis. The extracts were obtained from ethanolic extraction and their chemical composition was determined by high-performance liquid chromatography coupled to mass spectrometry. The cellular toxicity was measured in MDBK (Madin-Darby Bovine Kidney) cells and quantified by the MTT assay (3- (4,5 dimethylthiazol-2yl -2,5-diphenyl-2H bromine tetrazolato). For antifungal activity, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined by broth microdilution. The results showed that cell toxicity was not observed at lower concentrations (0.097 to 0.39μg/ml) for all extracts in comparison to cell control. Among the chemical compounds identified, caffeic acid, p-coumaric acid, chlorogenic acid, ferulic acid and rutin were quantified. In antifungal activity, green and jataí did not exhibit activity against the isolates (MIC and MFC greater than 0.78mg/ml). However, all isolates of S. brasiliensis were sensitive to brown propolis (MIC of 0.09 to 0.78mg/ml), including the standard strain (P<0.001). Among the Brazilian propolis studied, the brown propolis showed activity against the S. brasiliensis isolates and more studies should be undertaken in order to evaluate its promising use in the treatment of sporotrichosis.(AU)

Neste estudo, descreveu-se a atividade antifúngica de três extratos de própolis brasileiras: marrom, verde e de abelhas jataí (Tetragonisca angustula), contra Sporothrix brasiliensis. Os extratos foram obtidos de extração etanólica, e a sua composição química foi determinada por cromatografia líquida de alta eficiência, acoplada à espectrometria de massa. A toxicidade celular foi medida em células MDBK (Madin-Darby Bovine Kidney), avaliada por observação microscópica e quantificada pelo ensaio MTT (3- (4,5-dimetiltiazol-2-ilo -2,5-difenil-2H bromo tetrazolato). Para a atividade antifúngica, determinou-se a concentração inibitória mínima (CIM) e a concentração fungicida mínima (CFM) por meio de microdiluição em caldo. Os resultados mostraram que a toxicidade celular não foi observada em concentrações menores (0,097 a 0,39μg/ml). Entre os compostos químicos identificados, foram quantificados o ácido cafeico, ácido p-cumárico, ácido clorogênico, ácido ferúlico e a rutina. Na atividade antifúngica, as própolis verde e jataí não apresentaram atividade contra os isolados (CIM e CFM maior que 0,78mg/ml), porém todos os isolados de S. brasiliensis foram sensíveis à própolis marrom (CIM de 0,09 a 0,78mg/ml), incluindo a cepa padrão (P<0,001). Entre as própolis brasileiras estudadas, a marrom mostrou atividade contra S. brasiliensis, e mais estudos devem ser realizados para avaliar seu uso promissor no tratamento da esporotricose.(AU)
Descritores: Própole/análise
Própole/uso terapêutico
Sporothrix/isolamento & purificação
-Itraconazol/uso terapêutico
Farmacorresistência Fúngica
Apiterapia/veterinária
Antifúngicos/análise
Limites: Humanos
Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-976167
Autor: Corona Carpio, Marcia Hortensia; López Verdecia, Charles Anthony; Duharte Escalante, Ana; Ramón Jimenez, Ruth; Tamayo Hernández, Yailen.
Título: Efectividad de la tintura de propóleos al 10 % en pacientes con estomatitis subprótesis / Effectiveness of 10 % propolis tinction in patients with subprosthesis stomatitis
Fonte: Medisan;22(9), nov.-dic. 2018. tab.
Idioma: es.
Resumo: Se efectuó un estudio de intervención terapéutica en 50 pacientes con estomatitis subprótesis, quienes acudieron a la consulta de prótesis de la Clínica Estomatológica Ramón Guevara Montano de Baracoa, provincia de Guantánamo, desde abril del 2015 hasta junio del 2016, con vistas a determinar la efectividad del tratamiento con tintura de propóleos al 10 por ciento en los afectados. Se conformaron 2 grupos: uno de estudio (A) y otro de control (B), con 25 integrantes cada uno; el primero fue tratado con propóleos y el segundo con medicina alopática (nistatina en suspensión). La evaluación se realizó a los 4, 8 y 12 días. Al finalizar la terapia se observó que en 100,0 por ciento de los pacientes del grupo A se eliminó la lesión; por tanto, esta alternativa terapéutica resultó efectiva. Se recomendó extender dicha aplicación a otras afecciones bucales.

A study of therapeutic intervention was carried out in 50 patients with subprosthesis stomatitis who went to the Prosthesis department of "Ramón Guevara Montano" Stomatological Clinic in Baracoa, Guantánamo province, from April, 2015 to June, 2016, aimed at determining the effectiveness of the treatment with 10 percent propolis tinction in the affected patients. Two groups were formed: a study group (A) and a control group (B), with 25 members each; the first one was treated with propolis tinction and the second with allopathic medicine (nystatin in suspension). The evaluation was carried out at 4.8 and 12 days. When concluding the therapy it was observed that in 100.0 percent of the patients of the group A the lesion was eliminated; therefore, this therapeutic alternative was effective. It was recommended to extend this procedure to other oral disorders.
Descritores: Própole/uso terapêutico
Estomatite sob Prótese/terapia
Candidíase/terapia
-Avaliação de Eficácia-Efetividade de Intervenções
Apiterapia
Mel
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Tipo de Publ: Artigo Clássico
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: biblio-910794
Autor: Martínez, Romina Paola.; Feldman, Jorge; Álvarez, Marcelo.
Título: Utilización de medicina complementaria en procesos degenerativos articulares / Use of complementary medicine in joint degenerative processes.
Fonte: Tandil; s.n; 2016. 39 p. ilus, tab.
Idioma: es.
Tese: Apresentada a Universidad Nacional del Centro de la Provincia de Buenos Aires para obtenção do grau de Especialista.
Resumo: En la clínica alopática vemos a diario el avance de nuevos tratamientos hacia diversas patologías. Sin embargo, se ha visto que en las terapias crónicas de enfermedades inflamatorias degenerativas asociadas a la edad del paciente, (gerontes) el margen terapéutico de sus medicamentos se torna cada vez más estrecho, debido a que con el paso del tiempo aparecen disfunciones orgánicas que limitan la aplicación de fármacos sintéticos, por sus efectos colaterales. Es en esta brecha donde la medicina complementaria comienza a jugar un papel importante en el arsenal terapéutico del médico veterinario. En esta tesina, se describirán tres herramientas terapéuticas dentro de las llamadas medicinas complementarias que se utilizan en la terapia de los procesos degenerativos del aparato osteoarticular y se comparará su efecto de acción con las drogas antiinflamatorias de síntesis que se usan habitualmente. Las terapias que se desarrollarán en este trabajo son: La acupuntura, la fitomedicina y la apiterapia a través de la aplicación de apitoxina.
Descritores: Acupuntura
Terapias Complementares
Artropatias
-Apiterapia
Argentina
Doença Crônica
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-734617
Autor: Jantakee, Kanyaluck; Tragoolpua, Yingmanee.
Título: Activities of different types of Thai honey on pathogenic bacteria causing skin diseases, tyrosinase enzyme and generating free radicals
Fonte: Biol. Res;48:1-11, 2015. graf, tab.
Idioma: en.
Projeto: Chiang Mai University. Faculty of Science; . The Thailand Research Fund-Research and Researchers for Industry; . Bee Products Industry Co.
Resumo: BACKGROUND: Honey is a natural product obtained from the nectar that is collected from flowers by bees. It has several properties, including those of being food and supplementary diet, and it can be used in cosmetic products. Honey imparts pharmaceutical properties since it has antibacterial and antioxidant activities. The antibacterial and antioxidant activities of Thai honey were investigated in this study. RESULTS: The honey from longan flower (source No. 1) gave the highest activity on MRSA when compared to the other types of honey, with a minimum inhibitory concentration of 12.5% (v/v) and minimum bactericidal concentration of 25% (v/v). Moreover, it was found that MRSA isolate 49 and S. aureus were completely inhibited by the 50% (v/v) longan honey (source No. 1) at 8 and 20 hours of treatment, respectively. Furthermore, it was observed that the honey from coffee pollen (source No. 4) showed the highest phenolic and flavonoid compounds by 734.76 mg gallic/kg of honey and 178.31 mg quercetin/kg of honey, respectively. The antioxidant activity of the honey obtained from coffee pollen was also found to be the highest, when investigated using FRAP and DPPH assay, with 1781.77 mg FeSO4•7H2O/kg of honey and 86.20 mg gallic/kg of honey, respectively. Additionally, inhibition of tyrosinase enzyme was found that honey from coffee flower showed highest inhibition by 63.46%. CONCLUSIONS: Honey demonstrates tremendous potential as a useful source that provides anti-free radicals, anti-tyrosinase and anti-bacterial activity against pathogenic bacteria causing skin diseases.
Descritores: Apiterapia
Flavonoides/análise
Mel/análise
Monofenol Mono-Oxigenase
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Fenóis/análise
Pólen/química
-Antibacterianos/farmacologia
Antioxidantes/farmacologia
Compostos de Bifenilo
Café/química
Recuperação de Fluorescência Após Fotodegradação
Flores/química
Radicais Livres/análise
Mel/classificação
Testes de Sensibilidade Microbiana
Monofenol Mono-Oxigenase/antagonistas & inibidores
Monofenol Mono-Oxigenase/efeitos dos fármacos
Picratos
Pólen/classificação
Dermatopatias/microbiologia
Dermatopatias/terapia
Preparações Clareadoras de Pele/farmacologia
Tailândia
Fatores de Tempo
Viscosidade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: lil-675526
Autor: Ruiz Salvador, Ana Karelia; García Milián, Ana Julia; Jiménez López, Giset; Alfonso Orta, Ismary; Pérez Hernández, Bárbaro; Carrazana Lee, Armando.
Título: Farmacovigilancia de fitofármacos y apifármacos en Cuba durante 2006-2010 / Pharmacovigilance of phytomedicines and apimedicines in Cuba, 2006-2010
Fonte: Rev. cuba. plantas med;18(2):173-186, abr.-jun. 2013.
Idioma: es.
Resumo: Introducción: las plantas y los productos de la colmena han sido una importante fuente de medicinas durante un largo período, pero también pueden provocar reacciones adversas. Objetivo: caracterizar las reacciones adversas asociadas al uso de los fitofármacos y apifármacos que se comercializaron en Cuba en el período 2006-2010. Método: se realizó un estudio observacional, descriptivo y transversal. La muestra estuvo constituida por la totalidad de los reportes de sospechas de reacciones adversas a medicamentos, producidas por fitofármacos y apifármacos a la Unidad Coordinadora Nacional de Farmacovigilancia, durante el período de estudio. Resultados: la mayoría de los reportes de sospechas de reacciones adversas se distribuyeron en el sexo femenino (73,3 por ciento) y en el grupo etario de 31 a 60 años (52,5 por ciento). El ajo tintura y jarabe (13,4 por ciento), el Vimang® tableta y jarabe (9,7 por ciento), así como el eucalipto jarabe y tintura (6,6 por ciento) resultaron los fitofármacos más notificados, mientras que el propóleos tintura (3,4 por ciento) lo fue dentro de los apifármacos reportados. El sistema digestivo resultó el más afectado por las reacciones adversas, clasificadas mayormente de leves y probables. Conclusiones: la farmacovigilancia realizada a los fitofármacos y apifármacos en el período estudiado guarda relación con lo reportado en la literatura para estos productos

Introduction: plants and beehive products have been an important source of medicines for a long time, but they may also cause adverse reactions. Objective: characterize the adverse reactions associated with the use of phytomedicines and apimedicines sold in Cuba from 2006 to 2010. Method: a cross-sectional observational descriptive study was conducted. The sample consisted of all the reports on suspected adverse drug reactions caused by phytomedicines and apimedicines submitted to the National Pharmacovigilance Coordinating Unit during the study period. Results: most reports on suspected adverse reactions corresponded to the female sex (73.3 percent) and the 31-60 age group (52.5 percent). The phytomedicines most frequently reported were garlic tincture and syrup (13.4 percent), Vimang™ pills and syrup (9.7 percent), and eucalyptus syrup and tincture (6.6 percent), whereas the most frequently reported apimedicine was propolis tincture (3.4 percent). The digestive system was the most commonly affected by adverse reactions, which were mostly classified as mild or probable. Conclusions: pharmacovigilance of phytomedicines and apimedicines during the study period is consistent with the bibliographic information about the topic
Descritores: Apiterapia
Medicamentos Fitoterápicos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: lil-656728
Autor: Kapoor, Shailendra.
Título: Systemic benefits and potential uses of tualang honey in addition to its beneficial effects on postmenopausal bone structure
Fonte: Clinics;67(11):1345-1345, Nov. 2012.
Idioma: en.
Descritores: Apiterapia
Densidade Óssea/efeitos dos fármacos
Cálcio na Dieta/administração & dosagem
Suplementos Nutricionais
Mel
Limites: Animais
Feminino
Tipo de Publ: Comentário
Carta
Responsável: BR1.1 - BIREME


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Id: lil-645451
Autor: Zaid, Siti Sarah Mohamad; Sulaiman, Siti Amrah; Othman, Nor Hayati; Soelaiman, Ima-Nirwana; Shuid, Ahmad Nazrun; Mohamad, Norazlina; Muhamad, Norliza.
Título: Protective effects of Tualang honey on bone structure in experimental postmenopausal rats
Fonte: Clinics;67(7):779-784, July 2012. ilus, tab.
Idioma: en.
Projeto: Universiti Sains Malaysia.
Resumo: OBJECTIVE: The objective of this study was to evaluate the effects of Tualang honey on trabecular structure and compare these effects with those of calcium supplementation in ovariectomized rats. METHODS: Forty female, Sprague-Dawley rats were randomly divided into five groups (n =8): four controls and one test arm. The control arm comprised a baseline control, sham-operated control, ovariectomized control, and ovariectomized calcium-treated rats (receiving 1% calcium in drinking water ad libitum). The test arm was composed of ovariectomized, Tualang honey-treated rats (received 0.2 g/kg body weight of Tualang honey). Both the sham-operated control and ovariectomized control groups received vehicle treatment (deionized water), and the baseline control group was sacrificed without treatment. RESULTS: All rats were orally gavaged daily for six weeks after day one post-surgery. The bone structural analysis of rats in the test arm group showed a significant increase in the bone volume per tissue volume (BV/TV), trabecular thickness (Tb.Th) and trabecular number (Tb.N) and a significant decrease in inter-trabecular space (Tb.Sp) compared with the ovariectomized control group. The trabecular thickness (Tb.Th) in the test arm group was significantly higher compared with the ovariectomized-calcium treated group, and the inter-trabecular space (Tb.Sp) in the test arm group was significantly narrower compared with the ovariectomized-calcium treated group. CONCLUSION: In conclusion, ovariectomized rats that received Tualang honey showed more improvements in trabecular bone structure than the rats that received calcium.
Descritores: Apiterapia
Densidade Óssea/efeitos dos fármacos
Cálcio na Dieta/administração & dosagem
Suplementos Nutricionais
Mel
-Ovariectomia
Osteoporose/prevenção & controle
Distribuição Aleatória
Ratos Sprague-Dawley
Limites: Animais
Feminino
Ratos
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-608728
Autor: Faúndez Poblete, W; Narváez Carrasco, C. G; Burgos Arias, A.
Título: Efecto antiinflamatorio de apitoxina de Apis mellifera sobre prostaglandina E2 del fluido crevicular gingival de pacientes con y sin enfermedad periodontal, sometidos a apiterapia: ensayo preliminar / Anti-inflammatory effect of apitoxin and Apis mellifera on prostaglandin E2 in gingival crevicular fluid of patients with and without periodontal disease, submitted to apitherapy: preliminary test
Fonte: Rev. clín. periodoncia implantol. rehabil. oral (Impr.);4(2):64-68, ago. 2011. ilus, tab.
Idioma: es.
Resumo: Introducción: La apitoxina que es producida por la Apis mellifera posee efecto antiinflamatorio sobre una serie de marcadores biológicos. La prostaglandina E2 forma parte de ellos, estando presente en el fluido gingival crevicular (FGC). La prostaglandina E2 es evidenciada en la enfermedad periodontal. Objetivo: En este estudio se evaluó el efecto antiinflamatorio de la apitoxina sobre la concentración de prostaglandina E2 del FGC de un paciente sin enfermedad periodontal (SEP) y otro con enfermedad periodontal (CEP). Materiales y Método: Se seleccionó un paciente SEP y otro CEP, que sometidos a apiterapia durante 28 días, se registraron 5 muestras por paciente de FGC, siendo almacenadas, centrifugadas y refrigeradas para su conservación. Posteriormente se midió la concentración de prostaglandina E2 crevicular mediante inmunodifusión radial simple en placas petri con concentración de anticuerpo anti prostaglandina E2 de 1:1000. Selladas a 4°C, se esperó 72 horas para permitir su difusión, tiñéndose con Azul brillante de Coomasie, determinándose la concentración de cada placa. Resultados: Paciente SEP inmediatamente antes de apiterapia presentó una concentración de 0.9636 ± 0.0055 (ug/uL), finalizando con una concentración de 0.9196+/-0.0733 (ug/uL) al completar 28 días de tratamiento. El paciente CEP antes de recibir apiterapia presento una concentración de 1.1866 +/- 0.0867 (ug/uL), finalizando con una concentración de 0.9858 +/- 0.0074 (ug/uL) al completar 28 días de tratamiento. Discusión: Los hallazgos de este estudio demuestran una disminución de la concentración de PGE2 del FGC tanto para el paciente CEP y SEP sometidos a apiterapia durante 28 días, siendo esta disminución 3.7 veces mayor en el paciente CEP.

Introduction: Apitoxin, which is produced by Apis mellifera, has anti-inflammatory effect on a number of biomarkers. Prostaglandin E2 is one of them, being present in gingival crevicular fluid (GCF). Prostaglandin E2 is evidenced in periodontal disease. Objective: This study evaluated the antiinflammatory effect of apitoxin on concentration of prostaglandin E2 FGC in a patient with no periodontal disease (SEP) and other with periodontal disease (CEP). Materials and Methods: We selected both a SEP and CEP patient who were subjected to apitherapy for 28 days. There were 5 samples per patient of FGC, being stored, centrifuged and refrigerated for their preservation. Subsequently, the concentrations of crevicular prostaglandin E2 were measured by simple radial immunodiffusion in petri dishes with antibody concentration of prostaglandin E2 of 1:1000. Sealed at 4 °C, after 72 hours to allow diffusion, they were stained with Coomassie Brilliant Blue, determining the concentration of each plate. Results: SEP patient immediately before apitherapy presented a concentration of 0.9636 +/- 0.0055 (g / mL), ending with a concentration of 0.9196 +/- 0.0733 (g / mL) upon completion of 28 days of treatment. CEP patient before receiving apitherapy showed a concentration of 1.1866 +/- 0.0867 (g/mL), ending with a concentration of 0.9858 +/- 0.0074 (g/mL) upon completion of 28 days of treatment. Discussion: The findings of this study show a decrease in the concentration of PGE2 of FGC both for the CEP and SEP patient subjected to apitherapy for 28 days, being this decrease 3.7 times higher in the CEP patient.
Descritores: Anti-Inflamatórios
Apis mellifica
Dinoprostona/análise
Doenças Periodontais/metabolismo
Líquido do Sulco Gengival/química
-Apiterapia
Doenças Periodontais/terapia
Imunodifusão
Biomarcadores
Limites: Humanos
Adulto
Responsável: CL1.1 - Biblioteca Central



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