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Id: biblio-886194
Autor: Seitenfus, Rafael; Ferreira, Paulo Roberto Walter; Santos, Gabriel Oliveira dos; Alves, Rafael José Vargas; Kalil, Antonio Nocchi; Barros, Eduardo Dipp de; Glehen, Olivier; Casagrande, Thaís Andrade Costa; Bonin, Eduardo Aimoré; Silva Junior, Edison Martins da.
Título: A prototype single-port device for pressurized intraperitoneal aerosol chemotherapy. Technical feasibility and local drug distribution
Fonte: Acta cir. bras;32(12):1056-1063, Dec. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To evaluate the technical feasibility and homogeneity of drug distribution of pressurized intraperitoneal aerosol chemotherapy (PIPAC) based on a novel process of intraperitoneal drug application (multidirectional aerosolization). Methods: This was an in vivo experimental study in pigs. A single-port device was manufactured at the smallest diameter possible for multidirectional aerosolization of the chemotherapeutic drug under positive intraperitoneal pressure. Four domestic pigs were used in the study, one control animal that received multidirectional microjets of 9 mL/sec for 30 min and three animals that received multidirectional aerosolization (pig 02: 9 mL/sec for 30 min; pigs 03 and 04: 3 mL/sec for 15 min). Aerosolized silver nitrate solution was applied for anatomopathological evaluation of intraperitoneal drug distribution. Results: Injection time was able to maintain the pneumoperitoneum pressure below 20 mmHg. The rate of moderate silver nitrate staining was 45.4% for pig 01, 36.3% for pig 02, 36.3% for pig 03, and 72.7% for pig 04. Conclusions: Intra-abdominal drug distribution had a broad pattern, especially in animals exposed to the drug for 30 min. Our sample of only four animals was not large enough to demonstrate an association between aerosolization and a higher silver nitrate concentration in the stained abdominal regions.
Descritores: Neoplasias Peritoneais/tratamento farmacológico
Sistemas de Liberação de Medicamentos/métodos
Aerossóis/administração & dosagem
-Neoplasias Peritoneais/patologia
Neoplasias Peritoneais/secundário
Peritônio/efeitos dos fármacos
Pressão
Fatores de Tempo
Insuflação
Estudos de Viabilidade
Sistemas de Liberação de Medicamentos/instrumentação
Aerossóis/farmacocinética
Cavidade Abdominal
Sus scrofa
Modelos Animais de Doenças
Injeções Intraperitoneais
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-837654
Autor: Éboli, Lígia Patrícia de Carvalho Batista; Netto, Alcides Augusto Salzedas; Azevedo, Ramiro Antero de; Lanzoni, Valéria Pereira; Paula, Tatiana Sugayama de; Goldenberg, Alberto; Gonzalez, Adriano Miziara.
Título: Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine
Fonte: Acta cir. bras;31(12):783-792, Dec. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT PURPOSE: To describe an animal model for acute liver failure by intraperitoneal d-galactosamine injections in rats and to define when is the best time to intervene through King's College and Clichy´s criteria evaluation. METHODS: Sixty-one Wistar female rats were distributed into three groups: group 1 (11 rats received 1.4 g/kg of d-galactosamine intraperitoneally and were observed until they died); group 2 (44 rats received a dose of 1.4 g/kg of d-galactosamine and blood and histological samples were collected for analysis at 12 , 24, 48 , 72 and 120 hours after the injection); and the control group as well (6 rats) . RESULTS: Twelve hours after applying d-galactosamine, AST/ALT, bilirubin, factor V, PT and INR were already altered. The peak was reached at 48 hours. INR > 6.5 was found 12 hours after the injection and factor V < 30% after 24 hours. All the laboratory variables presented statistical differences, except urea (p = 0.758). There were statistical differences among all the histological variables analyzed. CONCLUSION: King's College and Clichy´s criteria were fulfilled 12 hours after the d-galactosamine injection and this time may represent the best time to intervene in this acute liver failure animal model.
Descritores: Falência Hepática Aguda/induzido quimicamente
Galactosamina
-Fatores de Tempo
Ratos Wistar
Falência Hepática Aguda/patologia
Falência Hepática Aguda/terapia
Apoptose/efeitos dos fármacos
Modelos Animais de Doenças
Injeções Intraperitoneais
Fígado/patologia
Limites: Animais
Feminino
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-797294
Autor: Hernández Villarroel, Luis Alfredo; Cesín, Luisa; Fernández, Henry.
Título: Efectos de la pentoxifilina en la formación de adherencias peritoneales postoperatorias en ratas / Pentoxifylline effects on the formation of postoperative peritoneal adhesions in rats
Fonte: Rev. cientif. cienc. med;19(1):5-11, 2016. ilus.
Idioma: es.
Resumo: Las adherencias son una consecuencia común y desafortunada de muchas de las cirugías abdominales. Algunos pacientes forman extensas adherencias permanentes que pueden causar dolor abdominal o pélvico, infertilidad y obstrucción intestinal. Los estudios epidemiológicos han puesto en evidencia la magnitud de este problema y el costo de los servicios de salud, y aunque hay numerosos enfoques para prevenir la formación de adherencias minimizando el daño peritoneal, el uso de barreras, diversos tópicos y agentes farmacológicos, ningún método hasta ahora ha resultado totalmente eficaz en ensayos aleatorios controlados. En este estudio, se decidió evaluar los efectos de la administración intraperitoneal de Pentoxifilina en la prevención de adherencias peritoneales postoperatorias en ratas.Veinte ratas hembras Sprague-Dawley, fueron sometidas a laparotomía. Las adherencias peritoneales postoperatorias fueron inducidas por abrasamiento de la superficie serosa del colon. Los animales fueron divididos al azar en dos grupos experimentales: un grupo que recibió Pentoxifilina, y el otro como grupo control. Y fueron sacrificados y evaluados a los 15 días, observándose una disminución en cuanto a número (p=0,025), severidad (p=0,0018), extensión (p=0,0013), densidad (p=0,0071), grado de inflamación (p=0,0020), proliferación vascular (p=0,0007) y fibrosis (p=0,0047) de las adherencias en el grupo tratado con Pentoxifilina, en relación al grupo control. En conclusión, este estudio demostró que la administración de Pentoxifilina por vía intraperitoneal disminuye de forma significativa la formación de adherencias peritoneales postoperatorias, y por tanto, puede ser útil en la prevención de las mismas.

The Adhesions are a common and unfortunate consequence of most abdominal surgical operations. Some patients form extensive permanent adhesions that can cause abdominal or pelvic pain, infertility and bowel obstruction. Epidemiological studies have highlighted the extent of this problem and the cost to the health service, and although there are numerous approaches to prevent adhesions formation by decreasing peritoneal injury, the use of various adhesion barriers and topical pharmacological agents, no method so far has proved completely efficacious in randomized controlled trials. In this study, it decided to evaluate the effects of peritoneal exposure to Pentoxifylline in post-surgical adhesions in rats. Twenty female Sprague-Dawley rats underwent laparotomy. Postoperative peritoneal adhesions was induced by scarping serosal the surface of the colon. The animals were divided randomly into two experimental groups: a group treated with Pentoxifylline, and a control group. And were killed and evaluated at 15 postoperative days, decrease was observed in the number (p=0,025), severity (p=0,0018), extension (p=0,0013),density (p=0,0071), inflammation (p=0,0020), vascular proliferation (p=0,0007) and fibrosis score (p=0,0047) of adhesions in the group treated with Pentoxifylline, when compare to control group. In conclusion, this study demonstrated that the administration of Pentoxifylline intraperitoneally decrease significantly the peritoneal adhesions formation, and thus, may be useful for its prevention of same.
Descritores: Pentoxifilina
-Ratos Sprague-Dawley
Injeções Intraperitoneais
Limites: Animais
Tipo de Publ: Ensaio Clínico
Responsável: BO138.1 - Biblioteca Central


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Id: lil-787429
Autor: Marcon, Raphael Martus; Cristante, Alexandre Fogaça; de Barros Filho, Tarcísio Eloy Pessoa; Ferreira, Ricardo; dos Santos, Gustavo Bispo.
Título: Effects of ganglioside G(M1) and erythropoietin on spinal cord lesions in rats: functional and histological evaluations
Fonte: Clinics;71(6):351-360tab, graf.
Idioma: en.
Resumo: OBJECTIVE: To evaluate the functional and histological effects of ganglioside G(M1) and erythropoietin after experimental spinal cord contusion injury. METHODS: Fifty male Wistar rats underwent experimental spinal cord lesioning using an NYU-Impactor device and were randomly divided into the following groups, which received treatment intraperitoneally. The G(M1) group received ganglioside G(M1) (30 mg/kg); the erythropoietin group received erythropoietin (1000 IU/kg); the combined group received both drugs; and the saline group received saline (0.9%) as a control. A fifth group was the laminectomy group, in which the animals were subjected to laminectomy alone, without spinal lesioning or treatment. The animals were evaluated according to the Basso, Beattie and Bresnahan (BBB) scale, motor evoked potential recordings and, after euthanasia, histological analysis of spinal cord tissue. RESULTS: The erythropoietin group had higher BBB scores than the G(M1) group. The combined group had the highest BBB scores, and the saline group had the lowest BBB scores. No significant difference in latency was observed between the three groups that underwent spinal cord lesioning and intervention. However, the combined group showed a significantly higher signal amplitude than the other treatment groups or the saline group (p<0.01). Histological tissue analysis showed no significant difference between the groups. Axonal index was significantly enhanced in the combined group than any other intervention (p<0.01). CONCLUSION: G(M1) and erythropoietin exert therapeutic effects on axonal regeneration and electrophysiological and motor functions in rats subjected to experimental spinal cord lesioning and administering these two substances in combination potentiates their effects.
Descritores: Eritropoetina/farmacologia
Gangliosídeo G(M1)/farmacologia
Fármacos Neuroprotetores/farmacologia
Recuperação de Função Fisiológica/efeitos dos fármacos
Traumatismos da Medula Espinal/tratamento farmacológico
-Quimioterapia Combinada
Eritropoetina/uso terapêutico
Gangliosídeo G(M1)/uso terapêutico
Injeções Intraperitoneais
Locomoção/efeitos dos fármacos
Modelos Animais
Necrose
Distribuição Aleatória
Ratos Wistar
Tempo de Reação/efeitos dos fármacos
Traumatismos da Medula Espinal/patologia
Traumatismos da Medula Espinal/fisiopatologia
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: lil-770487
Autor: Aun, Marcelo Vivolo; Saraiva-Romanholo, Beatriz Mangueira; Almeida, Francine Maria de; Brüggemann, Thayse Regina; Kalil, Jorge; Martins, Milton de Arruda; Arantes-Costa, Fernanda Magalhães; Giavina-Bianchi, Pedro.
Título: Sensitization by subcutaneous route is superior to intraperitoneal route in induction of asthma by house dust mite in a murine mode / Sensibilização por via subcutânea é superior à intraperitoneal na indução de asma por ácaro em modelo murino
Fonte: Einstein (Säo Paulo);13(4):560-566, Oct.-Dec. 2015. tab, graf.
Idioma: pt.
Resumo: ABSTRACT Objective To develop a new experimental model of chronic allergic pulmonary disease induced by house dust mite, with marked production of specific immunoglobulin E (IgE), eosinophilic inflammatory infiltrate in the airways and remodeling, comparing two different routes of sensitization. Methods The protocol lasted 30 days. BALB/c mice were divided into six groups and were sensitized subcutaneously or intraperitoneally with saline (negative control), Dermatophagoides pteronyssinus (Der p) 50 or 500mcg in three injections. Subsequently they underwent intranasal challenge with Der p or saline for 7 days and were sacrificed 24 hours after the last challenge. We evaluated the titration of specific IgE anti-Der p, eosinophilic density in peribronchovascular space and airway remodeling. Results Both animals sensitized intraperitoneally and subcutaneously produced specific IgE anti-Der p. Peribronchovascular eosinophilia increased only in mice receiving lower doses of Der p. However, only the group sensitized with Der p 50mcg through subcutaneously route showed significant airway remodeling. Conclusion In this murine model of asthma, both pathways of sensitization led to the production of specific IgE and eosinophilia in the airways. However, only the subcutaneously route was able to induce remodeling. Furthermore, lower doses of Der p used in sensitization were better than higher ones, suggesting immune tolerance. Further studies are required to evaluate the efficacy of this model in the development of bronchial hyperresponsiveness, but it can already be replicated in experiments to create new therapeutic drugs or immunotherapeutic strategies.

RESUMO Objetivo Desenvolver um novo modelo experimental de doença pulmonar alérgica crônica por ácaro, com proeminente produção de imunoglobulina E (IgE) específica, infiltrado inflamatório eosinofílico nas vias aéreas e remodelamento, comparando duas vias diferentes de sensibilização. Métodos O protocolo teve duração de 30 dias. Camundongos BALB/c foram divididos em seis grupos submetidos à sensibilização por via subcutânea ou intraperitoneal com solução salina (controles negativos),Dermatophagoides pteronyssinus (Der p) 50 ou 500mcg, em três aplicações. Posteriormente, foram submetidos à provocação intranasal com Der p ou salina por 7 dias e sacrificados 24 horas após o último desafio. Avaliamos a titulação de IgE específica anti-Der p, densidade eosinofílica no espaço peribroncovascular e remodelamento das vias aéreas. Resultados Tanto os animais sensibilizados por via subcutânea como intraperitoneal produziram IgE específica anti-Der p. Ocorreu aumento da eosinofilia peribroncovascular apenas nos animais que receberam menor dose de Der p. Porém apenas o grupo sensibilizado com Der p 50mcg subcutânea apresentou remodelamento significativo das vias aéreas. Conclusão Neste modelo murino de asma, as duas vias de sensibilização levaram à produção de IgE específica e eosinofilia nas vias aéreas. No entanto, apenas a via subcutânea foi capaz de induzir ao remodelamento. Além disso, doses menores de Der p utilizadas foram superiores às mais elevadas, sugerindo tolerância. Mais estudos são necessários para avaliar a eficácia deste modelo no desenvolvimento da hiperresponsividade brônquica, mas ele pode ser replicado em experimentos para criação de novas estratégias terapêuticas medicamentosas ou imunoterápicas.
Descritores: Alérgenos/administração & dosagem
Asma/imunologia
Modelos Animais de Doenças
Imunização/métodos
Pyroglyphidae
-Administração Intranasal
Asma/fisiopatologia
Testes de Provocação Brônquica
Ensaio de Imunoadsorção Enzimática
Eosinófilos/metabolismo
Colágenos Fibrilares/metabolismo
Injeções Intraperitoneais
Injeções Subcutâneas
Imunoglobulina E/sangue
Imunoglobulina G/sangue
Contagem de Leucócitos
Camundongos Endogâmicos BALB C
Anafilaxia Cutânea Passiva/imunologia
Eosinofilia Pulmonar/parasitologia
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: lil-761496
Autor: Zhang, Li-min; Song, Wen; Cui, Hao; Xing, Li-qiang; Du, Hui-bo; Cui, Ying; Chen, Wei-hong; Zhao, Zi-gang; Niu, Chun-yu.
Título: Normal mesenteric lymph ameliorates lipopolysaccharide challenge-induced spleen injury
Fonte: Acta cir. bras;30(9):604-610, Sep. 2015. ilus.
Idioma: en.
Projeto: Foundation of Hundred Innovative Talents in Universities of Hebei Province.
Resumo: PURPOSE: This study was conducted to investigate the effect of normal mesenteric lymph (NML) from mice on the spleen injury induced by lipopolysaccharide (LPS) challenge.METHODS: Mice in the LPS and LPS+NML groups received an intraperitoneal injection of LPS (35 mg/kg) and kept for 6 h.. The mice in the LPS+NML group received NML treatment at 1 h after LPS injection. Afterward, the splenic morphology, the levels of lipopolysaccharide-binding protein (LBP), cluster of differentiation 14 (CD14), phosphorylation mitogen-activated protein kinases (MAPKs), and inflammatory mediators in splenic tissue were investigated.RESULTS:LPS injection induced spleen injury, increased the levels of LBP, CD14, tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and interferon γ (IFN-γ), and decreased the IL-4 content in the spleen. By contrast, NML treatment reversed these changes. Meanwhile, the LPS challenge decreased the phosphorylation levels of p38 MAPK, extracellular regulated protein kinases 1/2, and c-Jun N-terminal kinase (JNK). Moreover, the phosphorylation levels of p38 MAPK and JNK were further decreased by the NML administration.CONCLUSION:rRdThe normal mesenteric lymph treatment alleviated lipopolysaccharide induced spleen injury by attenuating LPS sensitization and production of TNF-α, IL-6, and IFN-γ.
Descritores: Lipopolissacarídeos/administração & dosagem
Linfonodos/transplante
Mesentério
Esplenopatias/terapia
-Proteínas da Fase Aguda/análise
/análise
ANTIGENS, CDCONGENITAL ABNORMALITIES/análise
Proteínas de Transporte/análise
Citocinas/análise
Ensaio de Imunoadsorção Enzimática
Injeções Intraperitoneais
Camundongos Endogâmicos BALB C
Glicoproteínas de Membrana/análise
Quinases de Proteína Quinase Ativadas por Mitógeno/análise
Distribuição Aleatória
Reprodutibilidade dos Testes
Resultado do Tratamento
Limites: Animais
Tipo de Publ: Estudo de Avaliação
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-741244
Autor: Datta, Sanchita; Roy, Syamal; Manna, Madhumita.
Título: Therapy with radio-attenuated vaccine in experimental murine visceral leishmaniasis showed enhanced T cell and inducible nitric oxide synthase levels, suppressed tumor growth factor-beta production with higher expression of some signaling molecules
Fonte: Braz. j. infect. dis;19(1):36-42, Jan-Feb/2015. graf.
Idioma: en.
Projeto: Sanchita Datta, CSIR Senior Research Fellow.
Resumo: Background: Visceral leishmaniasis (VL) or Kala-Azar (KA) is one of the most deadly forms of disease among all neglected tropical diseases. There are no satisfactory drugs or vaccine candidates available for this dreaded disease. Our previous studies showed promising therapeutic and prophylactic efficacy of the live, radio-attenuated parasites through intramuscular (I.M.) and intraperitoneal (I.P.) route in BALB/c mice model. Methods: The T-cell proliferation level, the mRNA expression level of inducible nitric oxide synthase (iNOS) and tumor growth factor-beta (TGF-β) genes and finally the phosphorylation levels of phosphoinositide dependent kinase 1 (PDK1), phosphoinositide 3 kinase (PI3K) and p38 mitogen activated protein kinase (p38MAPK) molecules were checked in BALB/c mice model immunized with radio-attenuated Leishmania donovani parasites through I.M. route. Results: Higher T-cell proliferation, increased iNOS level, and suppressed TGF-β level were found in treated infected animal groups (100 and 150 Gy) in relation to untreated infected animals. Likewise, phosphorylation levels of PDK1, PI3K and p38MAPK of these two groups were increased when compared to untreated infected controls. Conclusion: The clearance of the parasites from treated infected groups of animals may be mediated by the restoration of T-cell due to therapy with radio-attenuated L. donovani parasites. The killing of parasites was mediated by increase in nitric oxide release through PDK1, PI3K and p38MAPK signaling pathways. A lower TGF-β expression has augmented the restored Th1 ambience in the 100 and 150 Gy treated animal groups proving further the efficacy of the candidate vaccine. .
Descritores: Vacinas contra Leishmaniose/imunologia
Leishmaniose Visceral/imunologia
-/genética
ABATTOIRS-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASES/genética
Western Blotting
Proliferação de Células
Modelos Animais de Doenças
Perfilação da Expressão Gênica
Injeções Intramusculares
Injeções Intraperitoneais
Vacinas contra Leishmaniose/administração & dosagem
Leishmaniose Visceral/prevenção & controle
Camundongos Endogâmicos BALB C
Óxido Nítrico Sintase Tipo II/genética
Carga Parasitária
Fosforilação
RNA Mensageiro
Células Th1/imunologia
Fator de Crescimento Transformador beta/genética
Vacinas Atenuadas/administração & dosagem
Vacinas Atenuadas/imunologia
/genética
PABREACTION MITOGEN-ACTIVATED PROTEIN KINASES/genética
Limites: Animais
Feminino
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Brentegani, Luiz Guilherme
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Id: lil-741214
Autor: Macedo, Rander Moreira; Brentegani, Luiz Guilherme; Lacerda, Suzie Aparecida de.
Título: Effects of Coffee Intake and Intraperitoneal Caffeine on Bone Repair Process - A Histologic and Histometric Study
Fonte: Braz. dent. j;26(2):175-180, Mar-Apr/2015. graf.
Idioma: en.
Resumo: Studies have suggested that caffeine acts on bone promoting an increase of calcium excretion, inhibition of osteoblast proliferation and delay in tissue repair process, raising the risk of fractures, osteoporosis, periodontal disease and affecting the success of bone reconstructive procedures. The aim of this study was to analyze histomorphometrically the process of alveolar bone healing after tooth extraction in rats subjected to daily intake of boiled coffee or intraperitoneal administration of caffeine. Forty-five male rats were divided according to the treatment in Control group (C); Coffee group (CO) - treated with coffee since birth; and Caffeine (CAF) - intraperitoneal injection of aqueous solution of caffeine 1.5% (0.2 mL/100g body weight) for 30 days. When weighing between 250-300 g they were anesthetized, subjected to extraction of the maxillary right incisor, and euthanized 7, 21 and 42 days after surgery for histological assessments of bone volume and the quality of formed bone in the dental socket. The qualitative results demonstrated larger amounts of blood clot and immature bone in animals under treatment of pure caffeine compared to coffee and control. Histometric analysis revealed that coffee treatment led to a 40% drop in bone formation, and caffeine a 60% drop in comparison to control animals (ANOVA p≤0.01). It was concluded that both the daily ingestion of coffee and the intraperitoneal administration of caffeine in rats delayed the alveolar bone reparative process after tooth extraction, and this effect was more aggressive when pure caffeine was used.

Estudos têm sugerido que a cafeína age sobre o osso promovendo um aumento da excreção de cálcio, inibição da proliferação dos osteoblastos e retardo no processo de reparação tecidual, aumentando o risco de fraturas, osteoporose, doença periodontal, bem como afetando o sucesso de procedimentos de reconstrução óssea. O objetivo deste estudo foi analisar histomorfometricamente o processo de reparação óssea alveolar após extração dentária em ratos submetidos à ingestão diária de café fervido ou a administração intraperitoneal de cafeína. 45 ratos machos foram divididos de acordo com o tratamento, em controle (C); café (CO), tratados com café desde o nascimento; e cafeína (CAF), injeção intraperitoneal de solução aquosa de cafeína de 1,5 % (0,2 mL/100 g de peso corporal) durante 30 dias. Quando pesavam entre 250-300 g os animais foram anestesiados, submetidos à extração do incisivo superior direito, e sacrificados em 7, 21 e 42 dias após a cirurgia para análises histológicas quanto ao volume e à qualidade do osso formado no alvéolo dental. Os resultados qualitativos demonstraram grandes quantidades de coágulo sanguíneo e osso imaturo nos animais tratados com cafeína pura, em relação aos grupos café e controle. A avaliação histométrica mostrou que o tratamento com o café levou a uma queda na formação óssea de 40%, e com a cafeína de 60% em comparação ao grupo controle (ANOVA p≤0,01). Concluiu-se que tanto a ingestão diária de café quanto a administração intraperitoneal de cafeína em ratos retardou o processo de reparação do osso alveolar após extração dentária, e este efeito é mais agressivo quando do uso da cafeína pura.
Descritores: Processo Alveolar/efeitos dos fármacos
Cafeína/farmacologia
Café/química
Osteogênese/efeitos dos fármacos
Cicatrização/efeitos dos fármacos
-Cafeína/administração & dosagem
Injeções Intraperitoneais
Ratos Wistar
Extração Dentária
Alvéolo Dental/efeitos dos fármacos
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Quevedo, Joäo
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Id: lil-710202
Autor: Rezin, Gislaine T.; Scaini, Giselli; Gonçalves, Cinara L.; Ferreira, Gabriela K.; Cardoso, Mariane R.; Ferreira, Andréa G.K.; Cunha, Maira J.; Schmitz, Felipe; Varela, Roger B.; Quevedo, João; Wyse, Angela T.S.; Streck, Emilio L..
Título: Evaluation of Na+, K+-ATPase activity in the brain of young rats after acute administration of fenproporex
Fonte: Rev. bras. psiquiatr;36(2):138-142, 13/05/2014. graf.
Idioma: en.
Resumo: Objectives: Fenproporex is an amphetamine-based anorectic which is rapidly converted into amphetamine in vivo. Na+, K+-ATPase is a membrane-bound enzyme necessary to maintain neuronal excitability. Considering that the effects of fenproporex on brain metabolism are poorly known and that Na+, K+-ATPase is essential for normal brain function, this study sought to evaluate the effect of this drug on Na+, K+-ATPase activity in the hippocampus, hypothalamus, prefrontal cortex, and striatum of young rats. Methods: Young male Wistar rats received a single injection of fenproporex (6.25, 12.5, or 25 mg/kg intraperitoneally) or polysorbate 80 (control group). Two hours after the last injection, the rats were killed by decapitation and the brain was removed for evaluation of Na+, K+-ATPase activity. Results: Fenproporex decreased Na+, K+-ATPase activity in the striatum of young rats at doses of 6.25, 12.5, and 25 mg/kg and increased enzyme activity in the hypothalamus at the same doses. Na+, K+-ATPase activity was not affected in the hippocampus or prefrontal cortex. Conclusion: Fenproporex administration decreased Na+, K+-ATPase activity in the striatum even in low doses. However, in the hypothalamus, Na+, K+-ATPase activity was increased. Changes in this enzyme might be the result of the effects of fenproporex on neuronal excitability. .
Descritores: Anfetaminas/administração & dosagem
Encéfalo/efeitos dos fármacos
Encéfalo/enzimologia
ATPase Trocadora de Sódio-Potássio/metabolismo
-Injeções Intraperitoneais
Ratos Wistar
Fatores de Tempo
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-709354
Autor: Shahryar, Habib Aghdam; Lotfi, Alireza.
Título: Effects of peptidic growth hormone secretagogue receptor (GHS-R) antagonist [D-Lys3] on some of serum hormonal and biochemical parameters in Wistar rat model / Efeitos do antagonista [D-Lys3] do receptor do peptídeo secretagogo do hormônio do crescimento (GHS-R) sobre alguns parâmetros bioquímicos e hormonais séricos em um modelo em ratos Wistar
Fonte: Arq. bras. endocrinol. metab;58(3):288-291, abr. 2014. tab, graf.
Idioma: en.
Resumo: Objective : The present study investigated the effects of different dosages of a GHS-R antagonist [D-Lys3] on some serum hormonal (cortisol, T3 and T4) and biochemical parameters in a rat.Materials and methods : Thirty-six 60-day-old male rats were assigned to four treatments. [D-Lys3]-GHRP-6 solutions were infused via intraperitoneal injections. Blood was collected and analyzed.Results : The large dosages of a GHS-R antagonist (200 ng/kg BW) caused increases in cortisol, whereas no significant changes occurred when low dosages were injected. There were no significant changes in T3 and T4 following the administration of the GHS-R antagonist, but a considerable increase was observed in blood glucose levels of the groups (G50, G100, and G200 ng/kg BW). There was a significant increase in total protein when the greatest dose was administrated (G200 ng/kg BW). However, total cholesterol, triglycerides, and albumin showed no significant changes.Conclusions : Exogenous GHS-R antagonist can cause an increase in glucose and moderate increases in cortisol and total protein, yet it has no significant effect on T3 and T4 levels or on the concentrations of serum lipids. The effect of GHS-R antagonist is not completely adverse to the effects of ghrelin. Further molecular studies are necessary to identify the physiological effects of the peptidic GHS-R antagonist. Arq Bras Endocrinol Metab. 2014;58(3):288-91.

Objetivo : O presente estudo investigou os efeitos de diferentes doses do antagonista do GHS-R [D-Lys3] sobre alguns parâmetros hormonais (cortisol, T3 e T4) e bioquímicos em ratos.Materiais e métodos : Trinta e seis ratos machos com 60 dias de idade foram alocados para quatro tratamentos. Soluções de [D-Lys3]-GHRP-6 foram administradas por meio de injeções intraperitoneais e foram coletadas e analisadas amostras.Resultados : Doses altas de antagonista de GHS-R (200 ng/kg PC) levaram a aumento do cortisol, enquanto não houve diferença significativa quando foram injetadas doses baixas. Não houve alterações significativas em T3 e T4 depois da administração do antagonista do GHS-R, mas foi observado aumento considerável nos níveis de glicose sanguínea dos grupos (G50, G100 e G200 ng/kg PC). Houve aumento significativo na proteína total quando foi administrada a maior dose (G200 ng/kg PC), entretanto, não foram observadas alterações no colesterol total, nos triglicérides e na albumina.Conclusões : O antagonista do GHS-R exógeno pode causar aumento da glicose e aumento moderado do cortisol e proteína total, embora não haja efeitos significativos nos níveis de T3 e T4 ou na concentração de lipídios séricos. O efeito do antagonista de GHS-R não é completamente adverso aos efeitos da grelina. Devem ser feitos outros estudos moleculares para se identificar os efeitos fisiológicos do peptídeo antagonista do GHS-R. Arq Bras Endocrinol Metab. 2014;58(3):288-91.
Descritores: Hidrocortisona/sangue
Oligopeptídeos/administração & dosagem
Receptores de Grelina/antagonistas & inibidores
Tiroxina/sangue
Tri-Iodotironina/sangue
-Glicemia/análise
HDL-Colesterol/sangue
Injeções Intraperitoneais
Distribuição Aleatória
Ratos Wistar
Albumina Sérica/análise
Triglicerídeos/sangue
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME



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