Base de dados : LILACS
Pesquisa : E02.319.267.530.620.570.100 [Categoria DeCS]
Referências encontradas : 6 [refinar]
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Id: lil-548244
Autor: De Guglielmo-Cróquer, Z; Altosaar, I.
Título: Transformation of coffee (Coffea Arabica L. cv. Catimor) with the cry1ac gene by biolistic, without the use of markers / Transformação do café (Coffea arabica L. cv. Catimor) com o gene cry1ac por bombardeamento, sem usar marcadores
Fonte: Braz. j. biol;70(2):387-393, May 2010. ilus, tab.
Idioma: en.
Resumo: The transformation of coffee plantlets with the cry1ac gene of Bacillus thuringiensis was achieved by biolistic using either the whole pUBC plasmid or only the ubi-cry1ac-nos genetic cassette. The cry1ac gene was inserted into coffee plants in order to confer resistance to the leaf miner Leucoptera coffeella, an insect responsible for considerable losses in coffee crops. Bearing in mind that the genetic cassettes used for this study lack reporter genes and/or selection marker genes, the parameters for the transformation procedure by biolistic were previously standardised with a plasmid carrying the gus reporter gene. The presence of the cry1ac gene in young plantlet tissues was determined by PCR, Southern blot and reverse transcription-PCR. Our results show that the obtainment of viable coffee plantlets, transformed by bombardment with the cry1ac gene and without selection markers nor reporter genes, is feasible.

A transformação das plântulas de café com o gene cry1ac de Bacillus thuringiensis foi realizada por biobalística, utilizando todo o pUBC plasmídeo ou só o cassete genético UBI-cry1ac-nos. O gene cry1ac foi inserido no cafeeiro a fim de conferir resistência à folha mineiro Leucoptera coffeella, um inseto responsável por perdas consideráveis nas culturas de café. Tendo em conta que ao plasmídeo e ao cassete genético utilizados para este estudo faltam genes repórteres e/ou de seleção, os parâmetros para o processo de transformação por biobalística foram previamente padronizados com um plasmídeo transportando o gene repórter gus. A presença do gene cry1ac em tecidos de jovens plântulas foi determinada por PCR, Southern blot e transcrição reversa-PCR. Nossos resultados mostram que a obtenção de plântulas de café, transformado por bombardeamento com o gene cry1ac sem genes de seleção genética nem repórteres é viável.
Descritores: Proteínas de Bactérias/genética
Coffea/genética
Endotoxinas/genética
Proteínas Hemolisinas/genética
Plantas Geneticamente Modificadas/genética
Transformação Genética/genética
-Western Blotting
Biolística/métodos
Lepidópteros
Reação em Cadeia da Polimerase
Doenças das Plantas/parasitologia
Doenças das Plantas/prevenção & controle
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  2 / 6 LILACS  
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Id: lil-501703
Autor: Valdez, Marta; Madriz, Kenneth; Ramírez, Pilar.
Título: Un método de transformación genética de maíz para conferirle resistencia ulterior a enfermedades virales / A method for genetic transformation of maize for resistance to viral diseases
Fonte: Rev. biol. trop;52(3):787-793, sept. 2004. ilus, tab.
Idioma: es.
Resumo: A system for the genetic transformation of maize was developed for two Costa Rican varieties: CR-7 and Diamantes 8843, that can allow the subsequent transfer of viral-derived genes in order to confer resistance to the disease caused by maize rayado fino virus (MRFV). The method is based on particle bombardment of organogenic calli derived from shoot tips. On the other hand, the molecular construction pRFcp-bar, containing the coat protein gene of MRFV and the marker gene bar, was elaborated. For the visual selection of the transformed material was used also the plasmid pDM803 that contains the reporter gene uidA (GUS). The results indicate that devices evaluated: the PIG ("Particle Inflow Gun") and the Bio-Rad are both enough efficient to transfer foreign genes to the genome of the maize.
Descritores: Plantas Geneticamente Modificadas/virologia
Transformação Genética/genética
Zea mays/genética
-Biolística
Costa Rica
Vírus de Plantas/genética
Vírus de Plantas/patogenicidade
Zea mays/virologia
Responsável: BR1.1 - BIREME


  3 / 6 LILACS  
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Azevedo, J. L
Coutinho, L. L
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Id: lil-290407
Autor: Ribeiro, L. A; Mariani, P. D. S. C; Azevedo, J. L; Rech, E. L; Schmidt, G. S; Coutinho, L. L.
Título: A biolistic process for in vitro gene transfer into chicken embryos
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;34(9):1115-1124, Sept. 2001. ilus, tab.
Idioma: en.
Projeto: FAPESP; . EMBRAPA/CNPSA; . CNPq.
Resumo: Chicken embryos kept in culture medium were bombarded using a high helium gas pressure biolistic device. To optimize the factors that affect transformation efficiency, the lacZ gene under control of the human cytomegalovirus immediate early enhancer/promoter was used as a reporter gene. There was an inverse relationship between survival rate and transformation efficiency. The best conditions obtained for high embryo survival and high transformation efficiency were achieved with 800 psi helium gas pressure, 500 mmHg vacuum, gold particles, an 8 cm DNA-coated microparticle flying distance to the embryo and embryo placement 0.5 cm from the center of the particle dispersion cone. Under these conditions, transformation efficiency was 100 percent, survival rate 25 percent and the number of expression units in the embryo body cells ranged from 100 to 1,000. Expression of green fluorescent protein was also detected in embryos bombarded under optimal conditions. Based on the results obtained, the biolistic process can be considered an efficient method for the transformation of chicken embryos and therefore can be used as a model system to study transient gene expression and tissue-specific promoters
Descritores: Biolística
Técnicas de Transferência de Genes
Técnicas In Vitro
-beta-Galactosidase/metabolismo
Expressão Gênica
Genes Reporter
Hélio
Indicadores e Reagentes/metabolismo
Óperon Lac
Proteínas Luminescentes/metabolismo
Plasmídeos
Pressão
Limites: Animais
Embrião de Galinha
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 6 LILACS  
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Azevedo, Joäo L
Coutinho, Luiz L
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Id: lil-254982
Autor: Ribeiro, Luciana A; Azevedo, Joäo L; Aragäo, Francisco J. L; Rech, Elibio L; Schmidt, Gilberto S; Coutinho, Luiz L.
Título: In situ DNA transfer to chicken embryos by biolistics
Fonte: Genet. mol. biol;22(4):525-9, Dec. 1999. ilus, tab.
Idioma: en.
Resumo: Ovos fertilizados de galinha foram bombardeados através da técnica de biobalística. A expressäo transiente do gene lacZ, sob o controle do promotor humano citomegalovírus, foi verificada após a transferência in situ. Diferentes níveis de pressäo de gás hélio, vácuo e tipos de partículas foram testados. A taxa de sobrevivência aumentou à medida que a velocidade das partículas diminuíram, entretanto, o nível de expressäo foi menor. Os melhores resultados, combinando taxa de sobrevivência e expressäo, foram obtidos com partículas de ouro, 600 libras por polegada ao quadrado de hélio e 600 mmHg de vácuo. Nestas condiçöes, todos os embriöes bombardeados apresentaram atividade da ß-galactosidase, indicando que esta técnica é eficiente para a transformaçäo de embriöes de galinha.
Descritores: Biolística
Embrião de Galinha
DNA
-beta-Galactosidase
Taxa de Sobrevida
Limites: Animais
Responsável: BR26.1 - Biblioteca Central


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Id: lil-228262
Autor: Oliveira, S. C; Rosinha, G. M. S; Brito, C. F. A. de; Fonseca, C. T; Afonso, R. R; Costa, M. C. M. S; Goes, A. M; Rech, E. L; Azevedo, V.
Título: Immunological properties of gene vaccines delivered by different routes
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;32(2):207-14, feb. 1999. tab, graf.
Idioma: en.
Conferência: Apresentado em: International Symposium The Third Revolution on Vaccines: DNA Vaccines, Belo Horizonte, Nov. 3-7 1997.
Resumo: Gene vaccines represent a new and promising approach to control infectious diseases, inducing a protective immune response in the appropriate host. Several routes and methods of genetic immunization have been shown to induce antibody production as well as T helper (Th) cell and cytotoxic T lymphocyte activation. However, few studies have compared the nature of the immune responses generated by different gene vaccination delivery systems. In the present study we reviewed some aspects of immunity induced by gene immunization and compared the immune responses produced by intramuscular (im) DNA injection to gene gun-mediated DNA transfer into the skin of BALB/c mice. Using a reporter gene coding for ß-galactosidase, we have demonstrated that im injection raised a predominantly Th1 response with mostly IgG2a anti-ßgal produced, while gene gun immunization induced a mixed Th1/Th2 profile with a balanced production of IgG2a and IgG1 subclasses. Distinct types of immune responses were generated by different methods of gene delivery. These findings have important implications for genetic vaccine design. Firstly, a combination between these two systems may create optimal conditions for the induction of a broad-based immune response. Alternatively, a particular gene vaccine delivery method might be used according to the immune response required for host protection. Here, we describe the characteristics of the immune response induced by gene vaccination and the properties of DNA involved in this process
Descritores: Genes
Imunoterapia Ativa/métodos
Vacinas de DNA/imunologia
-Biolística
Técnicas de Transferência de Genes
Camundongos Endogâmicos BALB C
Limites: Animais
Camundongos
Tipo de Publ: Revisão
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-186172
Autor: Rech, E. L; De-Bem, A. R; Aragäo, F. J. L.
Título: Biolistic-mediated gene expression in guinea pigs and cattle tissues in vivo
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;29(10):1265-7, Oct. 1996. ilus, tab.
Idioma: en.
Resumo: Foreign genes were introduced and expressed in vivo in guinea pigs and cattle utilizing a new hand-held device based on high-pressure helium gas to accelerate DNA-coated microparticles. Guinea pigs were used to evaluate the physical parameters to introduce and express the exogenous DNA. The best conditions were applied to conduct bombardments in cattle. The results showed a high frequency of gene expression in all the bombarded cattle. This procedure could be used to study the immune responses in cattle and in a wide variety of animals through genetic immunization.
Descritores: beta-Galactosidase/genética
Biolística/estatística & dados numéricos
Expressão Gênica/genética
-Imunização
Limites: Cobaias
Bovinos
Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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