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Id: biblio-951809
Autor: Paz, Jorge Enrique Wong; Contreras, Carolina Rubio; Munguía, Abigail Reyes; Aguilar, Cristóbal Noé; Inungaray, María Luisa Carrillo.
Título: Phenolic content and antibacterial activity of extracts of Hamelia patens obtained by different extraction methods
Fonte: Braz. j. microbiol;49(3):656-661, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Hamelia patens, is a plant traditionally used to treat a variety of conditions among the Huastec people of Mexico. The objective of this study is to characterize the phenolic content and critically examine the antimicrobial activity of leaf extracts H. patens, obtained by maceration, Soxhlet and percolation, using ethanol as 70% solvent. Phenolic compounds are characterized by liquid chromatography, coupled to a High Resolution Mass Spectrometry, and the antimicrobial activity was studied from the inhibitory effect of each extract for Escherichia coli, Staphylococcus aureus, Salmonella typhi and S. paratyphi, and by the Minimum Bactericidal Concentration, the percentage of activity and the Index of Bacterial Susceptibility of each extract. The phenolic compound identified in different concentrations in the three extracts was epicatechin. The extracts obtained by the three methods had antimicrobial activity, however, there was no significant difference (p < 0.05) between the Minimum Bactericidal Concentration of the extracts obtained by maceration, percolation and Soxhlet. The results of this study contribute to the body of knowledge on the use of extracts in controlling microorganisms with natural antimicrobials.
Descritores: Fenóis/isolamento & purificação
Fenóis/farmacocinética
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/farmacocinética
Hamelia/química
Fracionamento Químico/métodos
Antibacterianos/isolamento & purificação
Antibacterianos/farmacologia
-Fenóis/química
Staphylococcus aureus/efeitos dos fármacos
Extratos Vegetais/química
Testes de Sensibilidade Microbiana
Escherichia coli/efeitos dos fármacos
México
Antibacterianos/química
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-894172
Autor: El-Aziz, Tarek Mohamed Abd; Khoury, Sawsan Al; Jaquillard, Lucie; Triquigneaux, Mathilde; Martinez, Guillaume; Bourgoin-Voillard, Sandrine; Sève, Michel; Arnoult, Christophe; Beroud, Rémy; Waard, Michel De.
Título: Actiflagelin, a new sperm activator isolated from Walterinnesia aegyptia venom using phenotypic screening
Fonte: J. venom. anim. toxins incl. trop. dis;24:2, 2018. graf, ilus.
Idioma: en.
Projeto: Agence Nationale de la Recherche to the LabEx Ion Channels, Science and Therapeutics.
Resumo: Sperm contains a wealth of cell surface receptors and ion channels that are required for most of its basic functions such as motility and acrosome reaction. Conversely, animal venoms are enriched in bioactive compounds that primarily target those ion channels and cell surface receptors. We hypothesized, therefore, that animal venoms should be rich enough in sperm-modulating compounds for a drug discovery program. Our objective was to demonstrate this fact by using a sperm-based phenotypic screening to identify positive modulators from the venom of Walterinnesia aegyptia. Methods Herein, as proof of concept that venoms contain interesting compounds for sperm physiology, we fractionated Walterinnesia aegyptia snake venom by RP-HPLC and screened for bioactive fractions capable of accelerating mouse sperm motility (primary screening). Next, we purified each compound from the positive fraction by cation exchange and identified the bioactive peptide by secondary screening. The peptide sequence was established by Edman sequencing of the reduced/alkylated compound combined to LC-ESI-QTOF MS/MS analyses of reduced/alkylated fragment peptides following trypsin or V8 protease digestion. Results Using this two-step purification protocol combined to cell phenotypic screening, we identified a new toxin of 7329.38 Da (actiflagelin) that activates sperm motility in vitro from OF1 male mice. Actiflagelin is 63 amino acids in length and contains five disulfide bridges along the proposed pattern of disulfide connectivity C1-C5, C2-C3, C4- C6, C7-C8 and C9-C10. Modeling of its structure suggests that it belongs to the family of three finger toxins with a noticeable homology with bucandin, a peptide from Bungarus candidus venom. Conclusions This report demonstrates the feasibility of identifying profertility compounds that may be of therapeutic potential for infertility cases where motility is an issue.(AU)
Descritores: Motilidade Espermática
Espermatozoides/química
Venenos Elapídicos/isolamento & purificação
Venenos Elapídicos/uso terapêutico
Fosfolipases A2
-Acetilcolinesterase
Espectrometria de Massas em Tandem/métodos
Fracionamento Químico/métodos
Camundongos
Limites: Animais
Masculino
Ratos
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: biblio-899814
Autor: Moscoso-Mujica, Gladys; Zavaleta, Amparo; Mujica, Ángel; Santos, Marco; Calixto, Robert.
Título: Fraccionamiento y caracterización electroforética de las proteínas de la semilla de kañihua (Chenopodium pallidicaule Aellen) / Fractionation and electrophoretic characterization of (Chenopodium pallidicaule Aellen) kanihua seed proteins
Fonte: Rev. chil. nutr;44(2):144-152, 2017. ilus, graf, tab.
Idioma: es.
Resumo: Kanihua (Chenopodium pallidicaule Aellen) is a Chenopodiacea of the andean region, that contains between 15 and 19% protein, with essential amino acids. The objective of the study was to fractionate and electrophoretically characterize the proteins of kanihua seed varieties Cupi-Sayhua and Ramis. In the whole meal, the proximal analysis and fractionation were performed, and the flour was fractionated by five techniques according to Osborne solubility to obtain albumins, globulins, prolamins andglutelins. The methodology, solvents and extraction time were optimized; and the electrophoretic profiles of the fractions were identified. The highest protein content (p≤ 0.05) was of kanihua flour and its protein fractions, compared to kiwicha and wheat. The highest percent yield (p≤ 0.05) during 1 h of sequential extraction of the protein fractions, was obtained with the Rodriguez y et.al., technique for albumins and glutelins, and with the technique described by Barba de la Rosa y et.al., for globulins and prolamins. The following results were foundin Ramis and Cupi-Sayhua kanihua: albumins: 15.4±0.3 and 15.8±0.3%, globulins 7S: 24.1±0.5 and 26.3±1.0%, globulins 11S: 25.7±1.0 and 26.7±1.0%, prolamins: 9.6±0.1 and 9.9±0.5% and glutelins: 22.9±0.1 and 21.5±1.4%, respectively The electrophoretic profile showedpatterns similar in number of bands and differences in concentration in both varieties.

La kañihua (Chenopodium pallidicaule Aellen) es una Chenopodiacea de la región andina, que contiene entre 15 y 19% de proteínas, con aminoácidos esenciales. El objetivo del presente estudio fue fraccionar y caracterizar electroforéticamente las proteínas de la semilla de kañihua variedades Ramis y Cupi-Sayhua. En harina integral se realizó el análisis proximal y fraccionamiento, luego, la harina se fraccionó mediante cinco técnicas según la solubilidad de Osborne para obtener albuminas, globulinas, prolaminas y glutelinas. Se optimizó la metodología, solventes y tiempo de extracción; e identificaron los perfiles electroforéticos de las fracciones. El mayor contenido proteínico (p≤ 0,05) fue de la harina de kañihua y sus fracciones proteicas, en comparación a kiwicha y trigo. El mayor rendimiento porcentual (p≤ 0,05) durante 1 h de extracción secuencial de las fracciones proteicas, se obtuvo con la técnica de Rodríguez y et.al., para albuminas y glutelinas, y con la técnica de Barba de la Rosa y et.al., para globulinas y prolaminas. Se encontró en kañihua Ramis y Cupi-Sayhua, albúminas: 15,4±0,3 y 15,8+0,3%; globulinas: 7S 24,1±0,5 y 26,3+1,0%; globulinas 11S: 25,7+1,0 y 26,7+1,0%; prolaminas: 9,6+0,1 y 9,9+0,5% y glutelinas: 22,9+0,1 y 21,5+1,4%, respectivamente. El perfil electroforético mostró patrones similares en número de bandas y diferentes en concentración en ambas variedades.
Descritores: Proteínas
Grão Comestível
Eletroforese
Fracionamento Químico
-Valor Nutritivo
Tipo de Publ: Artigo Clássico
Responsável: CL334.1 - Biblioteca UBO


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Id: biblio-912161
Autor: Coser, Thais Rodrigues; Figueiredo, Cícero Célio de; Ramos, Maria Lucrécia Gerosa; Jannuzzi, Hermes; Marchão, Robélio Leandro.
Título: Recuperação de carbono obtida por três métodos em frações da matéria orgânica de latossolo, sob consórcio milho-forrageiras, no cerrado / Carbon recovery obtained by three methods in organic matter fractions of latosol under maize-grass intercropping in the cerrado
Fonte: Biosci. j. (Online);28(Supplement 1 - XXXIII Congresso Brasileiro de Ciência do Solo):91-97, 2012.
Idioma: pt.
Resumo: A dinâmica da matéria orgânica do solo (MOS) pode ser melhor compreendida após identificadas suas frações lábeis (> 53 µm) e estáveis (< 53 µm). Diante da importância crescente do uso de técnicas de fracionamento físico da MOS, torna-se necessário avaliar se a recuperação de carbono nessas frações é influenciada pelos métodos utilizados para sua determinação. O objetivo deste trabalho foi avaliar os teores de carbono, por três métodos, em diferentes frações da matéria orgânica, num Latossolo Vermelho-Amarelo distrófico sob consórcio milho-forrageiras e em sistema solteiro. Foram determinados os teores de carbono das frações: (i) particulada da matéria orgânica (COP) e naquela (ii) associada aos minerais da matéria orgânica (COM). Determinou-se ainda, o teor de (iii) carbono orgânico total do solo (COT) na matéria orgânica não fracionada. Três métodos de determinação do carbono orgânico do solo foram utilizados: (i) Walkley & Black modificado, (ii) Mebius modificado, e (iii) combustão via seca, em analisador elementar (CHN). Foram utilizadas amostras de solos de três sistemas de manejo dispostos em delineamento em blocos casualizados, com três repetições para determinação do carbono orgânico do solo: (i) milho solteiro; (ii) milho consorciado com capim colonião (Panicum maximum cv. Aruana); e (iii) milho consorciado com braquiária (Brachiaria humidicola). As amostras de solo foram coletadas na profundidade de 0 ­ 5 cm. O método Walkley & Black apresentou menores valores de COT em relação ao CHN nos sistemas de manejo milho/braquiária e milho solteiro, e mostrou valores de COP semelhantes àqueles obtidos pelo CHN nos sistemas de manejo milho solteiro e milho/braquiária. Valores de COT, COP e COM obtidos pela metodologia de Mebius modificado foram semelhantes àqueles obtidos pelo analisador elementar, para todos os sitemas de manejo. O COP, quando avaliado pelas metodologias de Mebius e CHN, mostrou-se significativamente mais sensível às alterações de manejo em relação ao COT e COM.

Soil organic matter alterations can be better understood when it is fractionated for the identification of its labile (> 53 µm) and stable (< 53 µm) fractions. Because of the increasing and important use of soil organic matter fractionation techniques, it becomes necessary to evaluate if the carbon recovery in these fractions is influenced by methods used for its determination. The aim of this work was to evaluate the content of organic carbon in soil organic matter fractions, by three different methods, under maize-grass intercropping and sole maize in a dystrophic Red-Yellow Latosol (Oxisol). The following carbon contents were determined from the fractions: (i) of the particulate soil organic matter (POM) and from that (ii) of mineral-associated soil organic matter (MOC). The content of total organic carbon (TOC) was also determined in the non-fractionated soil organic matter. Three methods for the determination of soil organic carbon were used: (i) Walkley & Black modified, (ii) Mebius modified, and (iii) dry combustion in a CHN elemental analyzer. The experimental design was a randomized block design with three replications. Soil samples were collected from three management systems: (i) sole corn; (ii) maize-Panicum maximum intercropping; and (iii) maize- Brachiaria humidicola intercropping. Soil samples were collected from the depth of 0 ­ 5 cm. Lower TOC were found in the Walkley & Black method in relation to the CHN elemental analyzer, under the management systems sole maize and maize-Panicum maximum intercropping. However, the Walkley & Black method showed similar POM in relation to CHN analyzer method, under the management systems sole maize and maize-Brachiaria humidicola intercropping. TOC, POM and MOC obtained from the Mebius modified method were similar to those obtained from the elemental analyzer, under the three management systems. The POM when evaluated by the Mebius and CHN methods, showed to be more sensible to soil management alterations.
Descritores: Carbono
Rotação de Cultivos
Matéria Orgânica
Fracionamento Químico
Responsável: BR396.4


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Id: biblio-886578
Autor: Quijano Parra, Alfonso; Quijano Vargas, Mónica Juliana; Meléndez Gélvez, Iván.
Título: Genotoxicidad de los hidrocarburos aromáticos policíclicos extraídos mediante el sistema diclorometano-etanol-tolueno en muestras del aire de Cúcuta, Norte de Santander, Colombia / Genotoxicity of polycyclic aromatic hydrocarbons extracted by dichloromethane-ethanol-toluene system air samples from Cúcuta, Norte de Santander, Colombia
Fonte: Acta toxicol. argent;25(1):1-11, mayo 2017. ilus, tab.
Idioma: es.
Resumo: Los contaminantes del aire han sido y siguen siendo, los principales factores que contribuyen a las enfermedades crónicas como el asma y enfermedades cardiovasculares. La contaminación del aire por material particulado (PM) es un problema mundial y en los últimos años, el PM se ha convertido en un tema importante de investigación ya que tiene un impacto negativo significativo en la salud humana; el PM es generado por las actividades industriales y tubos de escape de vehículos de motor. Sin embargo, diversos componentes nocivos del PM, como los hidrocarburos aromáticos policiclicos (HAP) en general, son sos­pechosos de ser carcinogénicos. Este trabajo tiene como objetivo identificar los HAP presentes en el PM2.5 del aire de Cúcuta, extraídos por primera vez, mediante el sistema diclorometano-etanol-tolueno e investigar la importancia del fraccionamiento de la materia organica del PM2.5 para detectar los HAP presentes en las fracciones del PM2.5. La identificación de los HAP considerados como contaminantes prioritarios y reconocidos por su afectación a la salud de la población se realizó, mediante cromatografía de gases con detector FID. Los efectos genotoxicos de la materia orgánica del PM2.5 extraída con una mezcla de DCM-etanol-tolueno fueron evaluados mediante el ensayo Cometa.

Air pollutants have been and still are the main factors that contribute to chronic diseases such as asthma and cardio­vascular disease. Air pollution by particulate matter (PM) is a global problem and in recent years, the PM has become an important research topic since it has a significant negative impact on human health; the PM is generated by industrial activities and exhaust pipes of motor vehicles. However, various harmful components of PM such as polycyclic aromatic hydrocarbons (PAHs) in gen­eral, are suspected of being carcinogenic. This work aims to identify the PAHs present in the PM 2.5 air Cúcuta, first extracted by the dichloromethane-ethanol-toluene system and investigate the importance of organic matter fractionation of PM 2.5 to detect PAHs present in the fractions of PM 2.5. The identification of PAHs considered as priority pollutants and recognized for their effects on health of the population was performed by gas chromatography with FID detector. The genotoxic effects of PM2.5 organic mat­ter, extracted with a mixture of DCM-ethanol-toluene, was evaluated by the Comet assay.
Descritores: Carcinógenos Ambientais
Genotoxicidade
Hidrocarbonetos Policíclicos Aromáticos/toxicidade
-Fracionamento Químico/métodos
Colômbia
Ensaio Cometa/métodos
Poluição Ambiental
Limites: Humanos
Tipo de Publ: Relatório Técnico
Responsável: AR1.1 - Biblioteca Rafael Herrera Vegas


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Lima, Edeltrudes de Oliveira
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Id: lil-775100
Autor: Neves, Michelline Viviane Marques das; Silva, Tânia Maria Sarmento da; Lima, Edeltrudes de Oliveira; Cunha, Emídio Vasconcelos Leitão da; Oliveira, Eduardo de Jesus.
Título: Isoflavone formononetin from red propolis acts as a fungicide against Candida sp
Fonte: Braz. j. microbiol;47(1):159-166, Jan.-Mar. 2016. tab, graf.
Idioma: en.
Resumo: Abstract A bioassay-guided fractionation of two samples of Brazilian red propolis (from Igarassu, PE, Brazil, hereinafter propolis 1 and 2) was conducted in order to determine the components responsible for its antimicrobial activity, especially against Candida spp. Samples of both the crude powdered resin and the crude ethanolic extract of propolis from both locations inhibited the growth of all 12 tested Candida strains, with a minimum inhibitory concentration of 256 µg/mL. The hexane, acetate and methanol fractions of propolis 1 also inhibited all strains with minimum inhibitory concentration values ranging from 128 to 512 µg/mL for the six bacteria tested and from 32 to 1024 µg/mL for the yeasts. Similarly, hexane and acetate fractions of propolis sample 2 inhibited all microorganisms tested, with minimum inhibitory concentration values of 512 µg/mL for bacteria and 32 µg/mL for yeasts. The extracts were analyzed by HPLC and their phenolic profile allowed us to identify and quantitate one phenolic acid and seven flavonoids in the crude ethanolic extract. Formononetin and pinocembrin were the major constituents amongst the identified compounds. Formononetin was detected in all extracts and fractions tested, except for the methanolic fraction of sample 2. The isolated isoflavone formononetin inhibited the growth of all the microorganisms tested, with a minimum inhibitory concentration of 200 µg/mL for the six bacteria strains tested and 25 µg/mL for the six yeasts. Formononetin also exhibited fungicidal activity against five of the six yeasts tested. Taken together our results demonstrate that the isoflavone formononetin is implicated in the reported antimicrobial activity of red propolis.
Descritores: Anti-Infecciosos/farmacologia
Candida/efeitos dos fármacos
Isoflavonas/isolamento & purificação
Isoflavonas/farmacologia
Própole/química
-Anti-Infecciosos/isolamento & purificação
Brasil
Bactérias/efeitos dos fármacos
Fracionamento Químico
Cromatografia Líquida de Alta Pressão
Testes de Sensibilidade Microbiana
Responsável: BR1.1 - BIREME


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Texto completo SciELO Venezuela
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Id: lil-752722
Autor: da Silva Pereira, Ana Carolina; Jair Wurlitzer, Nedio; Dionísio, Ana Paula; Lacerda Soares, Marcia Valéria; Rocha Bastos, Maria do Socorro; Elesbão Alves, Ricardo; Montenegro Brasil, Isabella.
Título: Synergistic, additive and antagonistic effects of fruit mixtures on total antioxidant capacities and bioactive compounds in tropical fruit juices / Efectos sinérgicos, aditivos y antagónicos de mezclas de pulpa frutas en la capacidad antioxidante total y compuestos bioactivos en los zumos de frutas tropicales
Fonte: Arch. latinoam. nutr;65(2):119-127, June 2015. ilus, tab.
Idioma: en.
Resumo: The objective of this work was investigate the synergistic, additive and antagonistic effects of fruit mixtures on total antioxidant capacities and bioactive compounds in tropical fruit juices, and optimize its formulation by the response surface methodology based on the responses: total polyphenols (TP), total antioxidant capacity (TAC), ascorbic acid content and sensorial acceptance. Camu-camu, acerola and acai were the major factors that influenced the antioxidant potential of the juice; and the yellow mombin showed a positive effect on the acceptance of the tropical juice. It was observed an antagonistic effect between acerola and camu-camu for the TAC response. The optimum formulation obtained was 20% acerola, 10% camu-camu, 10% yellow mombin, 10% cashew apple and 10% acai, which was responsible for a response of 155.46 mg.100 g-1 of ascorbic acid, 103.01 mg of GAE.100 g-1 of TP, 10.27 μM Trolox g-1 of TAC and approximately 6.1 of acceptance.

El objetivo de este trabajo fue investigar los efectos sinérgicos, aditivos y antagónicos de mezclas de diferentes frutas tropicales en la capacidad antioxidante total (TAC) y compuestos bioactivos presentes en los jugos mixtos, y optimizar su formulación por la metodología de superficie de respuesta basado en las evaluaciones de: polifenoles totales (TP), capacidad antioxidante total (TAC), contenido de ácido ascórbico y la aceptación sensorial. Camu-camu, acerola y acai fueron las frutas que más influyeron en el potencial antioxidante del jugo mixto; y el jobo mostró un efecto positivo en la aceptación del jugo mixto tropical. Se observó un efecto antagónico entre acerola y camu-camu para la TAC. La formulación óptima obtenida contenía 20% acerola, 10% de camu-camu, 10% el jobo, 10% de manzana de marañón y 10% de acai, la cual ha proporcionado contenidos medio de 155,46 mg.100 g-1 de ácido ascórbico, 103,01 mg de GAE.100 g-1 de TP, 10,27 mM Trolox g-1 de TAC y aproximadamente 6.1 de aceptación sensorial.
Descritores: Sucos de Frutas e Vegetais/análise
-Anacardiaceae/química
Anacardium/química
Ananas/química
Antioxidantes/análise
Ácido Ascórbico/análise
Fracionamento Químico/métodos
Interações Medicamentosas
Euterpe/química
Mangifera/química
Polifenóis/análise
Projetos de Pesquisa
Paladar
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Id: lil-740239
Autor: Imran, Samra; Hussain, Zaib; Ghafoor, Farkhanda; Ahmad Nagra, Saeed; Ashbeal Ziai, Naheeda.
Título: Comparative efficiency of different methods of gluten extraction in indigenous varieties of wheat / Eficacia comparativa de diferentes métodos de extracción de gluten en variedades autóctonas de trigo
Fonte: Arch. latinoam. nutr;63(2):180-187, June 2013. tab.
Idioma: en.
Resumo: The present study investigated six varieties of locally grown wheat (Lasani, Sehar, Miraj-08, Chakwal-50, Faisalabad-08 and Inqlab) procured from Punjab Seed Corporation, Lahore, Pakistan for their proximate contents. On the basis of protein content and ready availability, Faisalabad-08 (FD-08) was selected to be used for the assessment of comparative efficiency of various methods used for gluten extraction. Three methods, mechanical, chemical and microbiological were used for the extraction of gluten from FD-08. Each method was carried out under ambient conditions using a drying temperature of 55°C. Mechanical method utilized four different processes viz:- dough process, dough batter process, batter process and ethanol washing process using standard 150 mesh. The starch thus obtained was analyzed for its proximate contents. Dough batter process proved to be the most efficient mechanical method and was further investigated using 200 and 300 mesh. Gluten content was determined using sandwich ω-gliadin enzyme-linked immunosorbent assay (ELISA).The results of dough batter process using 200 mesh indicated a starch product with gluten content of 678 ppm. Chemical method indicated high gluten content of more than 5000 ppm and the microbiological method reduced the gluten content from 2500 ppm to 398 ppm. From the results it was observed that no gluten extraction method is viable to produce starch which can fulfill the criteria of a gluten free product (20ppm).

El presente roduc seis variedades de trigo cultivado localmente (Lasani, Sehar, Miraj-08, Chakwal-50, Faisalabad-08 e Inqlab) obtenidos por sus contenidos proximales en Punjab Seed Corporation, Lahore, Pakistán. Sobre la base del contenido de roduct y su fácil disponibilidad, Faisalabad-08 (Fd-08) fue seleccionado para ser utilizado para la evaluación de la eficacia comparadativa de los diferentes métodos utilizados para la extracción de gluten. Tres métodos fueron utilizados para la extracción de gluten; mecánico, químico y microbiológico realizados en condiciones ambientales utilizando una roduct e de secado de 55°C. El método mecánico roduct cuatro procesos diferentes, a saber: proceso de masa, proceso de rebozado de masa, proceso de pasta y proceso de lavado en etanol empleando malla estándar de 150 mesh. El almidón obtenido se analizó por sus contenidos proximales. El contenido de gluten se roduct usando roduct ω-gliadina, ensayo de inmunoabsorción ligado a enzimas (ELISA). El proceso de rebozado de masa fue el método roduct más eficiente y se investigó adicionalmente usando malla 200 y 300 mesh. El proceso de rebozado de masa usando malla 200 generó un almidón con contenido de gluten de 678 ppm. El método químico produjo un contenido de gluten de más de 5.000 ppm, y el método microbiológico redujo el contenido de gluten de 2500 ppm a 398 ppm. A partir de estos resultados se roduct que ningún de estos métodos de extracción de gluten fue es viable para roduct almidón que pueda cumplir los criterios de un roduct libre de gluten (20 ppm).
Descritores: Glutens/isolamento & purificação
Triticum/química
-Fracionamento Químico/métodos
Análise de Alimentos/métodos
Manipulação de Alimentos/métodos
Tipo de Publ: Estudo Comparativo
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Id: lil-665463
Autor: Castrillo Fernández, Azucena.
Título: Últimas técnicas de fraccionamiento y nuevos componentes sanguíneos / Novel fractionation techniques and new blood components
Fonte: Rev. argent. transfus;35(4):231-235, 2009.
Idioma: es.
Descritores: Células Sanguíneas
Preservação de Sangue/métodos
Preservação de Sangue/normas
Fracionamento Químico/métodos
-Excipientes Farmacêuticos/farmacologia
Bolsas Plásticas para Preservação de Sangue
Soluções Isotônicas/farmacologia
Soluções Isotônicas/uso terapêutico
Responsável: AR1.1 - Biblioteca Rafael Herrera Vegas


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Id: lil-661947
Autor: de Roodt, Adolfo Rafael; Litwin, Silvana; Estevez, Judith; Gould, Eduardo G; Dolab, Jorge A; Gould, Jorge.
Título: Comparación entre dos métodos de producción para la elaboración de antivenenos ofídicos / Snake antivenin: Comparison between two production methods
Fonte: Acta toxicol. argent;18(1):10-20, June 2010. ilus, tab.
Idioma: es.
Resumo: Las mordeduras producidas por serpientes venenosas son un serio problema médico en varias regiones del mundo y sobre las cuales los sistemas de salud actúan en diferentes grados en lo referente a tratamiento y prevención. Sin embargo, el tratamiento de las mordeduras de serpientes venenosas en animales domésticos puede resultar difícil por diversos motivos, siendo uno de estos la baja oferta o ausencia de antivenenos para uso veterinario. Las presiones comerciales en la industria farmacéutica han llevado a una reducción en la producción de antivenenos en varias partes del mundo, su disponibilidad es, a veces, bastante limitada y en algunos casos, son imposibles de conseguir. En este trabajo, inmunizamos caballos con veneno de serpientes Sudamericanas para obtener el plasma hiperinmune que fue procesado para obtener IgG entera o fragmentos F(ab´)2 usando dos métodos convencionales (fraccionamiento por ácido caprílico o doble precipitación salina y digestión con pepsina). Los antivenenos así obtenidos fueron probados en sus características bioquímicas e inmunoquímicas, así como en su potencia neutralizante. El SDS-PAGE de los antivenenos mostró bandas en el orden de los 150 y 100 kDa en los antivenenos conteniendo IgG entera o fragmentos F(ab´)2, respectivamente. La presencia de albúmina o contaminantes de alto o bajo peso molecular no fue detectada en ninguna de las preparaciones. No se observaron diferencias importantes en la potencia neutralizante de los antivenenos, aunque el costo de producción fue mucho más bajo en la obtención de IgG completa. A partir de esto, se sugiere que los bajos costos de producción en la obtención de antivenenos de IgG entera para uso veterinario, hacen a esta tecnología adecuada y rentable cuando la producción de F(ab´)2 no es posible.

Bites by venomous snakes are a serious medical problem in several regions of the world, on which the different health systems act with different modalities. Nevertheless, the treatment of venomous snakebites in domestic animals can turn difficult due several problems among which, the conspicuous, is the low availability or lack of antivenoms for veterinary use. As commercial pressures on the pharmaceutical industry have led to a reduction in the production of antivenins in several parts of the world, their availability is sometimes rather limited and sometimes these products are impossible to obtain. In this work, we immunized horses with venom of South American vipers to obtain hyperimmune plasma. The plasma was processed to separate whole IgG of F(ab´)2 fragments using two conventional methods (caprylic acid fractionation or double saline precipitation and pepsin digestion). The obtained antivenins were tested for their biochemical and immunochemical characteristics and neutralizing potency. The SDS-PAGE of the antivenins showed, in the processed antivenin, bands in the order of 150 and 100 kDa in the whole IgG or F(ab´)2 fragments, respectively. The presence of albumin or contaminants of high or low molecular weight was not detected in any of the preparations. No important differences were observed in the neutralizing potency of the antivenins, although production cost was very low with the method used to obtain pure IgG. The low production cost makes the production of antivenins for veterinary use profitable when the production of F(ab´)2 fragments is not possible.
Descritores: Antivenenos/uso terapêutico
Fragmentos Fab das Imunoglobulinas/farmacologia
Mordeduras de Serpentes
Venenos de Serpentes
-Caprilatos
Fracionamento Químico/métodos
Cavalos
Limites: Animais
Responsável: AR1.1 - Biblioteca Rafael Herrera Vegas



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