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Id: lil-768230
Autor: Avigliano, E.; Callicó-Fortunato, R.; Buitrago, J.; Volpedo, A. V..
Título: Is otolith microchemistry (Sr: Ca and Ba:Ca ratios) useful to identify Mugil curema populations in the southeastern Caribbean Sea? / A microquímica do otólito é um indicador do habitat de Mugil curema no sudeste do Mar Caribenho?
Fonte: Braz. j. biol;75(4,supl.1):45-51, Nov. 2015. tab, graf.
Idioma: en.
Projeto: Universidad de Buenos Aires; . ANPCyT; . CONICET.
Resumo: Abstract The aim of the present study was to evaluate the potential use of otolith microchemistry (Sr:Ca and Ba:Ca ratios) to identify silver mullet, Mugil curema, populations in Southeastern Caribbean Sea. Fish samples were collected in 7 areas of Nueva Esparta State (Venezuela). The otolith Sr:Ca and Ba:Ca ratios and water Sr:Ca were determined (by ICP-OES and EDTA volumetric method). Otoliths Sr:Ca and Ba:Ca ratios and Sr:Ca partition coefficient of mullets in Cubagua island (south of the State) were significantly different from ratios in La Guardia (north of the State). A discriminant analysis of otolith Sr:Ca and Ba:Ca ratios separated Cubagua Island from La Guardia values. These results suggest the existence of different mullet groups in the Southeastern Caribbean Sea. For this, the simultaneous use of Sr:Ca and Ba:Ca ratios could be a potential tool to identify populations in the study area.

Resumo O objetivo do presente estudo foi avaliar o potencial uso da microquímica do otólito (razões Sr:Ca e Ba:Ca) para identificar distintas populações de tainha, Mugil curema, no sudeste do mar caribenho. Os peixes foram coletados em 7 áreas do estado de Nueva Esparta (Venezuela). As razões Sr:Ca e Ba:Ca do otólito e a razão Sr:Ca da água foram determinadas (pelo ICP-OES e EDTA método volumétrico). As razões de Sr:Ca e Ba:Ca dos otólitos e o coeficiente de partição das tainhas da Ilha Cubagua (sul do estado) foram significativamente diferentes das razões de La Guardia (norte do estado). A análise discriminante das razões de Sr:Ca e Ba:Ca dos otólitos separa os valores da Ilha Cubagua e de La Guardia. Estes resultados sugerem a existência de diferentes grupos de Mugil curema no sudeste do mar Caribenho e que o uso simultâneo das razões Sr:Ca e Ba:Ca poderiam ser uma potencial ferramenta para identificar as populações da área de estudo.
Descritores: Bário/análise
Cálcio/análise
Membrana dos Otólitos/química
Smegmamorpha/fisiologia
Estrôncio/análise
-Região do Caribe
Ecossistema
Microquímica
Dinâmica Populacional
Venezuela
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-632349
Autor: Cortés-Sanabria, Laura; Martínez-Ramírez, Héctor R.; Hernández, José L.; Rojas-Campos, Enrique; Canales-Muñoz, José L.; Cueto-Manzano, Alfonso M..
Título: Utility of the Dipstick Micraltest II TM in the screening of microalbuminuria of diabetes mellitus type 2 and essential hypertension / Utilidad de la tira reactiva Micraltest II TMen el escrutinio de microalbuminuria en diabetes mellitus tipo 2 e hipertensión esencial
Fonte: Rev. invest. clín;58(3):190-197, June-May- 2006. ilus, tab.
Idioma: en.
Resumo: Background. In Mexico, diabetes mellitus type 2 and hypertension are leading causes of end-stage renal disease. Diagnosis of early renal damage with detection of microalbuminuria (microAlbU) is fundamental for treatment and prevention, and so avoiding the catastrophes of renal failure. For screening purposes, several simplified tests, including dipstick methods, fulfill the accuracy requirements for microAlbU detection compared with gold standards; however, no study has established the reliability of such tests in our setting. Aim. To evaluate the utility of micraltest II TM as a screening test for microAlbU compared with nephelometry in patients with diabetes mellitus type 2 and non-diabetic patients with essential hypertension. Patients and methods. Patients with diabetes mellitus type 2 as well as patients with essential hypertension of any age, sex and time of evolution, attending to three primary health-care units (UMF No. 3, 92 and 93, Guadalajara, Jalisco) were included. Patients with transitory albuminuria, secondary hypertension and serum creatinine > 2 mg/dL were excluded. Micraltest II TM was performed in the first morning urine sample, and nephelometry was performed in a 24-h urine collection. Diagnostic accuracy of the dipstick test was then determined. Results. 245 patients were studied: 71 (29%) were diabetics without hypertension, 95 (39%) were diabetics with hypertension, and 79 (32%) had only essential hypertension. In diabetic patients, micraltest II TM sensitivity was 83%, specificity 96%, and positive and negative predictive values were 95% and 88%, respectively. Correlation between nephelometry and micraltest II TM results was 0.81 (p < 0.001). The best cut-off point for microAlbU was 30.5 mg/L, and area under the curve (± SEM) was 0.91 ± 0.03 (confidence interval 95%: 0.85-0.96). In non-diabetic patients with essential hypertension, micraltest II TM sensitivity was 75%, specificity 95%, and positive and negative predictive values were 43% and 99%, respectively. Correlation between nephelometry and micraltest II TM results was 0.43 (p < 0.001). The best cut-off point for microAlbU was 28.2 mg/L, and area under the curve was 0.85 ± 0.13 (0.60-1.10). Conclusion. Micraltest II TM dispstick is a rapid, valid and reliable method for albuminuria screening in patients with diabetes mellitus type 2 and in those non-diabetic patients with essential hypertension in our setting.

Antecedentes. En México, la diabetes mellitus tipo 2 y la hipertensión son las principales causas de insuficiencia renal crónica terminal. El diagnóstico temprano con detección de microalbuminuria (microAlbU) es fundamental para el tratamiento y prevención, y así evitar las catástrofes de la falla renal. Con el fin de tamizaje, varias pruebas simples, incluyendo las tiras reactivas, cumplen con los requerimientos de exactitud para detección de microAlbU comparados con esténdares de oro; sin embargo, ningún estudio ha establecido la confiabilidad de dichos métodos en nuestro medio. Objetivo. Evaluar la utilidad del micraltest II TM como prueba de tamizaje para microAlbU comparada con nefelometría en pacientes con diabetes mellitus tipo 2 y pacientes no diabáticos con hipertensión arterial esencial. Pacientes y métodos. Se incluyeron pacientes con diabetes mellitus tipo 2, así como pacientes con hipertensión arterial esencial de cualquiera de los dos sexos, sexo y tiempo de evolución que atendían a tres unidades de Medicina Familiar (UMF No. 3, 92 y 93, Guadalajara, Jalisco). Se excluyeron pacientes con albuminuria transitoria, hipertensión secundaria y creatinina sárica > 2 mg/dL. El micraltest II TM se realizó en la primera muestra matutina de orina, y la nefelometría en recolecciones de orina de 24 horas. La exactitud diagnóstica de la tira reactiva fue luego determinada. Resultados. Doscientos cuarenta y cinco pacientes fueron estudiados: 71 (29%) eran diabáticos sin hipertensión, 95 (39%) eran diabáticos con hipertensión, y 79 (32%) tenían sólo hipertensión arterial esencial. En los pacientes diabáticos, el micraltest II TM tuvo una sensibilidad de 83%, especificidad de 96%, y valores predictivos positivo y negativo de 95% y 88%, respectivamente. La correlación entre la nefelometría y el micraltest II TM fue 0.81 (p < 0.001). El mejor punto de corte para la detección de microAlbU fue 30.5 mg/L, y el área bajo la curva (± EE) fue 0.91 ± 0.03 (intervalo de confianza 95%: 0.85-0.96). En los pacientes no diabáticos con hipertensión esencial, el micraltest II TM tuvo una sensibilidad de 75%, especificidad de 95%, y valores predictivos positivo y negativo de 43 y 99%, respectivamente. La correlación entre los resultados de nefelometría y micraltest II TM fue 0.43 (p < 0.001). El mejor punto de corte para microAlbU fue 28.2 mg/L, y el área bajo la curva fue 0.85 ± 0.13 (intervalo de confianza 95%:0.60-1.10). Conclusión. La tira reactiva micraltest II TM es un método rápido, válido y confiable para el tamizaje de albuminuria en pacientes con diabetes mellitus tipo 2 y pacientes no diabáticos con hipertensión arterial esencial en nuestro medio.
Descritores: Albuminúria/urina
/urina
DIABETES MELLITUS, TYPE TEMEFOS/urina
Hipertensão/urina
Programas de Rastreamento/métodos
Fitas Reagentes
-Albuminúria/etiologia
Estudos Transversais
/complicações
DIABETES MELLITUS, TYPE TEMEFOS/complicações
Hipertensão/complicações
Microquímica
Nefelometria e Turbidimetria
Valor Preditivo dos Testes
Amostragem
Sensibilidade e Especificidade
Limites: Idoso
Feminino
Seres Humanos
Masculino
Meia-Idade
Tipo de Publ: Estudo Comparativo
Estudos de Avaliação
Responsável: MX1.1 - CENIDSP - Centro de Información para Decisiones en Salud Pública


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Id: lil-591863
Autor: González-Nilo, Fernando; Pérez-Acle, Tomás; Guínez-Molinos, Sergio; Geraldo, Daniela A; Sandoval, Claudia; Yévenes, Alejandro; Santos, Leonardo S; Laurie, V. Felipe; Mendoza, Hegaly; Cachau, Raúl E.
Título: Nanoinformatics: an emerging area of information technology at the intersection of bioinformatics, computational chemistry and nanobiotechnology
Fonte: Biol. Res;44(1):43-51, 2011. ilus.
Idioma: en.
Projeto: ACTION-GRID project; . PBCT. Anillo Cientifico; . Fondecyt; . Instituto Milenio Centro Interdisciplinario de Neurociencias de Valparaiso.
Resumo: After the progress made during the genomics era, bioinformatics was tasked with supporting the flow of information generated by nanobiotechnology efforts. This challenge requires adapting classical bioinformatic and computational chemistry tools to store, standardize, analyze, and visualize nanobiotechnological information. Thus, old and new bioinformatic and computational chemistry tools have been merged into a new sub-discipline: nanoinformatics. This review takes a second look at the development of this new and exciting area as seen from the perspective of the evolution of nanobiotechnology applied to the life sciences. The knowledge obtained at the nano-scale level implies answers to new questions and the development of new concepts in different fields. The rapid convergence of technologies around nanobiotechnologies has spun off collaborative networks and web platforms created for sharing and discussing the knowledge generated in nanobiotechnology. The implementation of new database schemes suitable for storage, processing and integrating physical, chemical, and biological properties of nanoparticles will be a key element in achieving the promises in this convergent field. In this work, we will review some applications of nanobiotechnology to life sciences in generating new requirements for diverse scientific fields, such as bioinformatics and computational chemistry.
Descritores: Disciplinas das Ciências Biológicas
Biologia Computacional/tendências
Microquímica
Informática Médica/métodos
Nanotecnologia/tendências
-Simulação por Computador
Modelos Moleculares
Informática Médica/tendências
Limites: Seres Humanos
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Carmona, Maria José C
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Id: lil-453280
Autor: Sanches, Cristina; Galas, Filomena R. B. G; Silva, Anne G. O. de M; Carmona, Maria Jose C; Auler Júnior, José Otavio; Santos, Silvia Regina Cavani Jorge.
Título: Propranolol plasma monitoring in children submitted to surgery of tetralogy of Fallot by a micromethod using high performance liquid chromatography
Fonte: Clinics;62(3):215-224, June 2007. tab, graf.
Idioma: en.
Projeto: Brazilian Foundation for Research.
Resumo: OBJECTIVE: To evaluate the analytical micromethod using liquid chromatography for the quantification of propranolol in children submitted to surgery of tetralogy of Fallot (TLF). Methods: Only 0.2 mL of plasma is required for the assay. Peaks eluted at 8.4 (Propranolol) and 17.5 min (verapamil, internal standard) from a C18 column, with a mobile phase 0.1 M acetate buffer, pH 5.0, and acetonitrile (60:40, v/v) at flow rate 0.7 mL/min, detected at 290 nm (excitation) and 358 nm (emission). Surgery was started 776 min of drug administration (8.7mg, mean); seven blood samples were collected from six patients (4M/2F; 2.1yrs;11.5kg; 0.80m; 18.9kg/m²). RESULTS: Confidence limits of the method showed high selectivity and recovery, sensitivity of 0.02ng/mL, good linearity (0.05-1000ng/mL), precision of 8.6 percent and accuracy of 3.1 percent. The mean duration of surgery was 283.2min, with the patients remaining under cardiopulmonary bypass (CPB) for 114min. A declining curve of propranolol plasma concentration was obtained after the last dose in the night that preceded the day of surgery. Plasma concentration also was normalized with hematocrit due to the hemodilution caused by the CPB procedure. On the other hand a decrease on drug plasma concentration was obtained between periods, the beginning of surgery to the postoperative day 2 (7.09 ng/mL and 0.05 ng/mL, p<0.05 respectively) and from the end of CPB to the postoperative day 2 (2.79ng/mL e 0.05ng/mL, p<0.05). CONCLUSION: Propranolol monitoring of plasma concentrations of children (TLF) normalized after the last preoperative dose revealed a decline from the beginning of surgery to the second postoperative day, suggesting that, once redistribution was restored, propranolol washout was complete.

OBJETIVO: Avaliar o micrométodo analítico empregando a cromatografia líquida para quantificação de propranolol em crianças operadas de tetralogia de Fallot (TLF). MÉTODO: Requereu-se apenas volumes de 0,2mL de plasma para a realização do ensaio. Os picos foram eluídos em 8.4 (Propranolol) e 17.5 min (verapamil, padrão interno) de uma coluna C18, com fase móvel (tampão acetato 0,1 M pH 5,0 e acetonitrila, 60:40, v/v) em fluxo de 0,7 mL/min, sendo detectados em 290 nm (excitação) e em 358 nm (emissão). A cirurgia iniciou-se 776 min depois da dose administrada (8,7mg, média) e sete amostras de sangue foram coletadas de seis pacientes (4M/2F; 2,1 anos;11,5kg; 0,80m;18,9kg/m²). RESULTADOS: Os limites de confiança do método analítico evidenciaram alta seletividade e recuperação, sensibilidade (0,02ng/mL), boa linearidade (0,05-1000ng/mL), precisão de 8,6 por cento e exatidão de 3,1 por cento. A duração média da cirurgia foi de 283,2min, com os pacientes em circulação extracorpórea (CEC) durante 114min. Uma curva de declínio do propranolol no plasma foi obtida após a última dose na noite que precedeu o dia da intervenção. A concentração plasmática foi normalizada com o hematócrito devido à hemodiluição causada pela CEC. Por outro lado obteve-se decréscimo nas concentrações plasmáticas entre os períodos início da cirurgia para o 2° dia de pós-operatório (7,09 ng/mL e0,05 ng/mL, p<0,05 respectivamente) e do final da CEC para o 2° dia de pós-operatório (2,79ng/mL e 0,05ng/mL, p<0,05). CONCLUSÃO: O monitoramento das concentrações plasmáticas normalizadas do propranolol, em crianças com TLF, após a última dose pré-operatória revelou decaimento do início da cirurgia para o segundo pós-operatório, sugerindo que após a correção cirúrgica, uma vez restaurada a distribuição, a eliminação do fármaco foi completa.
Descritores: Microquímica/métodos
Propranolol/sangue
Tetralogia de Fallot/cirurgia
Vasodilatadores/sangue
-Cromatografia Líquida de Alta Pressão
Monitoramento de Medicamentos/métodos
Assistência Perioperatória
Propranolol/farmacocinética
Propranolol/uso terapêutico
Padrões de Referência
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Tetralogia de Fallot/sangue
Vasodilatadores/farmacocinética
Vasodilatadores/uso terapêutico
Limites: Pré-Escolar
Feminino
Seres Humanos
Lactente
Masculino
Tipo de Publ: Estudos de Avaliação
Research Support, Non-U.S. Gov't
Estudos de Validação
Responsável: BR1.1 - BIREME


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Id: lil-398182
Autor: Sampaio, L. F. S; Hamassaki-Britto, D. E; Markus, R. P.
Título: Influence of melatonin on the development of functional nicotinic acetylcholine receptors in cultured chick retinal cells
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;38(4):603-613, Apr. 2005. ilus, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: The influence of melatonin on the developmental pattern of functional nicotinic acetylcholine receptors was investigated in embryonic 8-day-old chick retinal cells in culture. The functional response to acetylcholine was measured in cultured retina cells by microphysiometry. The maximal functional response to acetylcholine increased 2.7 times between the 4th and 5th day in vitro (DIV4, DIV5), while the Bmax value for [125I]-alpha-bungarotoxin was reduced. Despite the presence of alpha8-like immunoreactivity at DIV4, functional responses mediated by alpha-bungarotoxin-sensitive nicotinic acetylcholine receptors were observed only at DIV5. Mecamylamine (100 µM) was essentially without effect at DIV4 and DIV5, while dihydro-ß-erythroidine (10-100 µM) blocked the response to acetylcholine (3.0 nM-2.0 µM) only at DIV4, with no effect at DIV5. Inhibition of melatonin receptors with the antagonist luzindole, or melatonin synthesis by stimulation of D4 dopamine receptors blocked the appearance of the alpha-bungarotoxin-sensitive response at DIV5. Therefore, alpha-bungarotoxin-sensitive receptors were expressed in retinal cells as early as at DIV4, but they reacted to acetylcholine only after DIV5. The development of an alpha-bungarotoxin-sensitive response is dependent on the production of melatonin by the retinal culture. Melatonin, which is produced in a tonic manner by this culture, and is a key hormone in the temporal organization of vertebrates, also potentiates responses mediated by alpha-bungarotoxin-sensitive receptors in rat vas deferens and cerebellum. This common pattern of action on different cell models that express alpha-bungarotoxin-sensitive receptors probably reflects a more general mechanism of regulation of these receptors.
Descritores: Melatonina/farmacologia
Receptores Nicotínicos/biossíntese
Retina/metabolismo
-Bungarotoxinas/metabolismo
Bungarotoxinas/farmacologia
Células Cultivadas
Imuno-Histoquímica
Microquímica
Antagonistas Nicotínicos/farmacologia
Receptores Nicotínicos/efeitos dos fármacos
Retina/citologia
Retina/efeitos dos fármacos
Fatores de Tempo
Triptaminas/farmacologia
Limites: Animais
Embrião de Galinha
Responsável: BR1.1 - BIREME


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Toma, Henrique E
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Id: lil-259473
Autor: Toma, Henrique E.
Título: Supramolecular chemistry and technology
Fonte: An. acad. bras. ciênc;72(1):5-25, mar. 2000.
Idioma: en.
Resumo: Supramolecular chemistry deals with the association of several chemical species, in an organized way and according to well defined purposes. Based on a molecular engineering approach, supramolecular structures can be designed from pre-formed building blocks, providing a promising route from chemistry to molecular nanotechnology. New supramolecular systems have been assembled in our laboratory with the use of bridging unities such as tetrapyridylporphyrins, porphyrazines and polypyrazines, connecting transition metal complexes and clusters. These systems display a very exciting electrochemical and catalytic behavior, and interact with DNA, generating 1O2 and leading to efficient oxidative clivage for photodynamic terapy applications. Molecular interfaces have been developed, exhibiting photocurrent response in the presence of visible-UV light, and rectifying properties in the presence of electroactive species. Successful applications of the supramolecular species in chemical and bio-sensors have been developed.
Descritores: Metais/química
Microquímica
-Técnicas Biossensoriais
Compostos Inorgânicos/química
Substâncias Macromoleculares
Estrutura Molecular
Compostos Organometálicos/química
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME



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