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Id: biblio-1090060
Autor: Rodrigues, Matheus Scarpatto; Pieri, Bruno Luiz da Silva; Silveira, Gustavo de Bem; Zaccaron, Rubya Pereira; Venturini, Ligia Milanez; Comin, Vitor Hugo; Luiz, Karine Damian; Silveira, Paulo Cesar Lock.
Título: Reduction of oxidative stress improves insulin signaling in cardiac tissue of obese mice / Redução do estresse oxidativo melhora a sinalização da insulina em tecido cardíaco de camundongos obesos
Fonte: Einstein (Säo Paulo);18:eAO5022, 2020. graf.
Idioma: en.
Resumo: ABSTRACT Objective To evaluate the effects of oxidative stress on insulin signaling in cardiac tissue of obese mice. Methods Thirty Swiss mice were equally divided (n=10) into three groups: Control Group, Obese Group, and Obese Group Treated with N-acetylcysteine. After obesity and insulin resistance were established, the obese mice were treated with N-acetylcysteine at a dose of 50mg/kg daily for 15 days via oral gavage. Results Higher blood glucose levels and nitrite and carbonyl contents, and lower protein levels of glutathione peroxidase and phosphorylated protein kinase B were observed in the obese group when compared with their respective control. On the other hand, treatment with N-acetylcysteine was effective in reducing blood glucose levels and nitrite and carbonyl contents, and significantly increased protein levels of glutathione peroxidase and phosphorylated protein kinase B compared to the Obese Group. Conclusion Obesity and/or a high-lipid diet may result in oxidative stress and insulin resistance in the heart tissue of obese mice, and the use of N-acetylcysteine as a methodological and therapeutic strategy suggested there is a relation between them.

RESUMO Objetivo Avaliar os efeitos do estresse oxidativo sobre a sinalização da insulina em tecido cardíaco de camundongos obesos. Métodos Utilizaram-se 30 camundongos Swiss subdivididos igualmente (n=10) em três grupos: Grupo Controle, Grupo Obeso e Grupo Obeso Tratado com N-acetilcisteína. Após estabelecidas a obesidade e a resistência à insulina, os camundongos obesos foram tratados diariamente, durante 15 dias, via gavagem oral, com N-acetilcisteína na dose de 50mg/kg. Resultados Observaram-se maiores níveis de glicose sanguínea, conteúdos de nitrito e carbonil, e menores níveis proteicos de glutationa peroxidase e proteína quinase B fosforilada no Grupo Obeso quando comparado a seu respectivo controle. Por outro lado, o tratamento com N-acetilcisteína se mostrou eficiente em diminuir os níveis glicêmicos, os conteúdos de nitrito e carbonil, e aumentar significativamente os níveis proteicos de glutationa peroxidase e proteína quinase B fosforilada, quando comparados ao Grupo Obeso. Conclusão Obesidade e/ou dieta hiperlipídica levam a estresse oxidativo e à resistência à insulina no tecido cardíaco de camundongos obesos, e o uso da N-acetilcisteína como estratégia metodológica e terapêutica sugeriu haver relação entre ambos.
Descritores: Acetilcisteína/farmacologia
Resistência à Insulina/fisiologia
Sequestradores de Radicais Livres/farmacologia
Estresse Oxidativo/fisiologia
Dieta Hiperlipídica
Miocárdio/metabolismo
-Valores de Referência
Espectrofotometria
Glicemia/análise
Peso Corporal
Western Blotting
Espécies Reativas de Oxigênio/análise
Estresse Oxidativo/efeitos dos fármacos
Carbonilação Proteica
Fluoresceínas/análise
Limites: Humanos
Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME


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Id: biblio-1285548
Autor: Moreira, Tiago César Gouvêa; Agostinho, Luciana de Andrade.
Título: Analysis of the qPCR Assay Efficacy as Molecular Diagnostic in Patients with Chemotherapy and/or Antibiotic Therapy
Fonte: Braz. arch. biol. technol;64:e21190423, 2021. tab, graf.
Idioma: en.
Resumo: Abstract High sensitivity of qPCR assay can be compromised by the presence of PCR inhibitors in samples analyzed. The aim of this study was to analyze the RT-qPCR assay efficiency considering the RNA quality/quantity and the presence of PCR inhibitors in patients with chemotherapy and/or antibiotic therapy. We analyzed 60 samples using RT-qPCR from individuals suspected of leukemia and 44 samples were quantified by fluorimetry and spectrophotometry. The efficiency of the RT-qPCR assay was evaluated comparing the threshold cycle (Ct) from tested samples and the standard curve. The 260/280 and 260/230 ratios, the presence of PCR inhibitors and the amount of sample (ng) used in the RT-qPCR reaction can be associated with 56.8% (R²=0.56, p<0.05) in the Ct obtained. The decrease of the RT-qPCR efficiency can be explained in 42,8% due to the variation of the 260/280 ratio (R²=0.42,p<0.05). The presence of antibiotics in the blood sample can be associated in 11.3% with the variability of 260/280 ratio (R²=0.11,p<0.05). Presence of chemotherapeutic drugs in the blood sample was not correlated with Ct variation (p=0.17). The spectrophotometer determines a RNA quantification with 2.2 times higher than the fluorimeter (t=2.2, p=0,03) and this difference is correlated with the 260/280 ratio (R²=0.36, p<0.05). Samples with low purity had a reduction in the qPCR efficiency, although we did not observe false results.
Descritores: Reação em Cadeia da Polimerase/métodos
Inibidores da Síntese de Ácido Nucleico
Técnicas de Diagnóstico Molecular/métodos
-Espectrofotometria/instrumentação
Fluorometria/instrumentação
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-843175
Autor: Coletti Zabala, Tamara L; Zerbatto, María E; Perotti, Elda B. R; Smacchia, Ana M; Ombrella, Adriana; Pidello, Alejandro R.
Título: Efecto del metronidazol suplementado con hidroquinona en la adherencia de Lactobacillus acidophilus en células vaginales ovinas / Effect of metronidazole supplemented with hydroquinone on the adhesion of Lactobacillus acidophilus in ovine vaginal cells
Fonte: Rev. argent. microbiol;48(4):313-319, dic. 2016. ilus, graf, tab.
Idioma: en.
Resumo: This work demonstrates that the addition of metronidazole together with a ubiquitous quinone compound reduces adherence of Lactobacillus acidophilus to ovine vaginal cells. Spectrophotometric and voltammetric studies have shown that neoformed compounds were observed in these systems; there were also changes in their electroactive composition, and the oxidant status had a significantly higher value compared to the control (p < 0.05). Based on reduction potential (E; mV), the distribution of electroactive compound concentrations suggests that the compounds with low reduction potential induce this behavior, which would indicate that the addition of metronidazole with a ubiquitous quinone compound to the vaginal system might increase the reductive capacity of these systems. This work shows that the study of behavior and fluctuations of the redox compounds that compose the vaginal environment, in terms of concentration and species of redox molecules, must be hierarchized in order to better understand the early stages of colonization by microorganisms.

Este trabajo demuestra que la incorporación de metronidazol conjuntamente con un compuesto quinónico ubicuo disminuye la adherencia de Lactobacillus acidophilus a células vaginales ovinas. Los estudios espectrofotométricos y voltamétricos mostraron que en estos sistemas aparecieron compuestos neoformados y que hubo modificaciones en la composición electroactiva; asimismo, el estatus oxidante presentó un valor significativamente superior con respecto al control (p < 0,05). Según los potenciales de reducción (E; mV), la distribución de las concentraciones de los compuestos electroactivos muestra que los compuestos con bajos potenciales de reducción inducen este comportamiento. Esto indicaría que la incorporación de esta mezcla al sistema vaginal aumentaría su capacidad reductora. El trabajo muestra que el estudio del comportamiento y las fluctuaciones de los compuestos redox que componen el ambiente vaginal, en términos de concentración y especies moleculares, debe ser jerarquizado para comprender mejor las primeras etapas de la colonización de este ambiente por parte de los microorganismos.
Descritores: Doenças dos Ovinos/prevenção & controle
Doenças Vaginais/veterinária
Aderência Bacteriana/efeitos dos fármacos
Metronidazol/análise
-Oxirredução/efeitos dos fármacos
Espectrofotometria/métodos
Ovinos/microbiologia
Metronidazol/farmacologia
Limites: Animais
Tipo de Publ: Estudo de Avaliação
Estudo Observacional Veterinário
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-1047456
Autor: Romero-Borbón, Evelyn; Grajales-Hernández, Daniel; Armendáriz-Ruiz, Mariana; Ramírez-Velasco, Lorena; Rodríguez-González, Jorge Alberto; Cira-Chávez, Luis Alberto; Estrada-Alvarado, María Isabel; Mateos-Díaz, Juan Carlos.
Título: Type C feruloyl esterase from Aspergillus ochraceus: a butanol specific biocatalyst for the synthesis of hydroxycinnamates in a ternary solvent system
Fonte: Electron. j. biotechnol;35:1-9, sept. 2018. graf, tab.
Idioma: en.
Projeto: Fondo Mixto del Gobierno del Estado de Jalisco.
Resumo: Background: Aspergillus ochraceus was isolated from coffee pulp and selected as an interesting hydroxycinnamoyl esterase strain producer, using an activity microplate high-throughput screening method. In this work, we purified and characterized a new type C A. ochraceus feruloyl esterase (AocFaeC), which synthesized specifically butyl hydroxycinnamates in a ternary solvent system. Results: AocFaeC was produced by solid state fermentation, reaching its maximal activity (1.1 U/g) after 48 h of culture. After purification, the monomeric protein (34 kDa) showed a specific activity of 57.9 U/mg towards methyl ferulate. AocFaeC biochemical characterization confirmed its identity as a type C feruloyl esterase and suggested the presence of a catalytic serine in the active site. Its maximum hydrolytic activity was achieved at 40°C and pH 6.5 and increased by 109 and 77% with Ca2+ and Mg2+, but decreased by 90 and 45% with Hg2+ and Cu2+, respectively. The initial butyl ferulate synthesis rate increased from 0.8 to 23.7 nmol/min after transesterification condition improvement, using an isooctane:butanol:water ternary solvent system, surprisingly the synthesis activity using other alcohols was negligible. At these conditions, the synthesis specific activities for butyl p-coumarate, sinapinate, ferulate, and caffeate were 87.3, 97.6, 168.2, and 234 U/µmol, respectively. Remarkably, AocFaeC showed 5 folds higher butyl caffeate synthesis rate compared to type B Aspergillus niger feruloyl esterase, a well-known enzyme for its elevated activity towards caffeic acid esters. Conclusions: Type C feruloyl esterase from A. ochraceus is a butanol specific biocatalyst for the synthesis of hydroxycinnamates in a ternary solvent system
Descritores: Aspergillus ochraceus/enzimologia
Hidrolases de Éster Carboxílico/metabolismo
Ácidos Cumáricos/síntese química
-Solventes
Espectrofotometria
Hidrolases de Éster Carboxílico/isolamento & purificação
Cromatografia
Café
Butanóis
Eletroforese
Fermentação
Responsável: CL1.1 - Biblioteca Central


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Id: lil-757425
Autor: Guignone, Bruna Coser; Silva, Ludimila Karsbergen; Soares, Rodrigo Villamarim; Akaki, Emilio; Goiato, Marcelo Coelho; Pithon, Matheus Melo; Oliveira, Dauro Douglas.
Título: Color stability of ceramic brackets immersed in potentially staining solutions
Fonte: Dental press j. orthod. (Impr.);20(4):32-38, July-Aug. 2015. tab, ilus.
Idioma: en.
Resumo: OBJECTIVE: To assess the color stability of five types of ceramic brackets after immersion in potentially staining solutions.METHODS: Ninety brackets were divided into 5 groups (n = 18) according to brackets commercial brands and the solutions in which they were immersed (coffee, red wine, coke and artificial saliva). The brackets assessed were Transcend (3M/Unitek, Monrovia, CA, USA), Radiance (American Orthodontics, Sheboygan, WI, USA), Mystique (GAC International Inc., Bohemia, NY, USA) and Luxi II (Rocky Mountain Orthodontics, Denver, CO, USA). Chromatic changes were analyzed with the aid of a reflectance spectrophotometer and by visual inspection at five specific time intervals. Assessment periods were as received from the manufacturer (T0), 24 hours (T1), 72 hours (T2), as well as 7 days (T3) and 14 days (T4) of immersion in the aforementioned solutions. Results were submitted to statistical analysis with ANOVA and Bonferroni correction, as well as to a multivariate profile analysis for independent and paired samples with significance level set at 5%.RESULTS: The duration of the immersion period influenced color alteration of all tested brackets, even though these changes could not always be visually observed. Different behaviors were observed for each immersion solution; however, brackets immersed in one solution progressed similarly despite minor variations.CONCLUSIONS: Staining became more intense over time and all brackets underwent color alterations when immersed in the aforementioned solutions.

OBJETIVO: avaliar a estabilidade da cor de cinco tipos de braquetes cerâmicos após imersão em soluções potencialmente corantes.MÉTODOS: noventa braquetes foram divididos em 5 grupos (n = 18) de acordo com a marca comercial dos braquetes e as soluções em que foram imersos (café, vinho tinto, Coca-Cola e saliva artificial). Os baquetes avaliados foram Transcend (3M / Unitek, Monrovia, CA, EUA), Radiance (American Ortodontia, Sheboygan, WI, EUA), Mystique (GAC International Inc., Bohemia, NY, EUA) e Luxi II (Rocky Mountain Orthodontics, Denver, CO, EUA). Alterações cromáticas foram analisadas com o uso de um espectrofotômetro de reflectância e por inspecção visual, em cinco intervalos de tempo específicos. Os momentos de avaliação foram: 24 horas (T1), 72 horas (T2), 7 dias (T3) e 14 dias (T4) de imersão nas soluções. Os resultados foram submetidos à avaliação estatística com análise de variância e correção de Bonferroni, bem como a uma análise do perfil multivariada para amostras independentes e pareadas, com nível de significância de 5%.RESULTADOS: a duração do período de imersão influenciou na alteração de cor de todos os braquetes testados, mesmo admitindo-se que essas alterações nem sempre puderam ser observadas visualmente. Diferentes comportamentos foram observados em cada solução de imersão; no entanto, braquetes imersos em um mesmo tipo de solução progrediram de forma semelhante, apesar das pequenas variações.CONCLUSÕES: a coloração se tornou mais intensa com o tempo, e todos os braquetes sofreram alteração de cor nas soluções imersas.
Descritores: Cerâmica/química
Braquetes Ortodônticos
Materiais Dentários/química
-Saliva Artificial/química
Espectrofotometria/instrumentação
Propriedades de Superfície
Fatores de Tempo
Vinho
Teste de Materiais
Bebidas Gaseificadas
Café
Cor
Corantes/química
Óxido de Alumínio/química
Imersão
Responsável: BR1.1 - BIREME


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Id: biblio-1089826
Autor: Hammad, Shaza M; El-Wassefy, Noha A; Alsayed, Mohamed A.
Título: Evaluation of color changes of white spot lesions treated with three different treatment approaches: an in-vitro study
Fonte: Dental press j. orthod. (Impr.);25(1):26-27, Jan.-Feb. 2020. tab, graf.
Idioma: en.
Resumo: ABSTRACT Objective: To qualitatively and quantitatively assess the color changes effect and the color stability of the resin infiltrant on white spot lesions (WSLs), in comparison with nano-hydroxyapatite (nano-HA) toothpaste and microabrasion. Methods: WSLs were artificially created on sixty human premolars enamel surfaces and randomly assigned to equal four groups (n = 15 each): nano-HA toothpaste, microabrasion (Opalusture), resin infiltrant (Icon) treatment, or artificial saliva (control group). The color change (ΔE) of each specimen was measured by dental spectrophotometer (Vita Easyshade) at different time points: baseline, after WSLs' creation, after application of treatments, one month, three and six months after treatments application. Results: The ΔE value did not differ significantly for the four groups at baseline measurement before treatment (p> 0.05). Icon resin infiltrant improved the color of WSLs significantly immediately after its application, giving the lowest ΔE value (3.00 ± 0.59), when compared to other treatments (p< 0.001). There were no significant changes in ΔE (p> 0.05) for all groups during the follow up intervals (one month, three and six months after treatments application). Conclusion: Resin infiltrant can improve the color of WSLs and restore the natural appearance of enamel better than nano-HA toothpaste and microabrasion.

RESUMO Objetivo: avaliar qualitativamente e quantitativamente os efeitos nas mudanças e estabilidade da cor de lesões de mancha branca (LMBs), após tratamento com infiltração de resina, em comparação aos tratamentos com pasta de dentes com nanopartículas de hidroxiapatita (Nano-HA) e com microabrasão. Métodos: As LMBs foram criadas artificialmente em 60 superfícies de esmalte dentário de pré-molares humanos e aleatoriamente divididas em quatro grupos (n=15, cada): pasta de dentes Nano-HA, microabrasão (Opalusture), tratamento com infiltração de resina (Icon) e saliva artificial (grupo controle). A mudança de cor (ΔE) de cada espécime foi aferida com um espectrofotômetro odontológico (Vita Easyshade) em diferentes tempos: início do estudo, após a criação das LMBs, após a aplicação dos tratamentos, um mês, três meses e seis meses após a aplicação dos tratamentos. Resultados: Os valores de ΔE não diferiram significativamente entre os quatro grupos ao início do estudo (p > 0,05). A infiltração com resina Icon melhorou significativamente a coloração das LMBs imediatamente após a sua aplicação, com o menor valor de ΔE (3,00 ± 0,59), quando comparada às outras modalidades de tratamento (p < 0,001). Não houve mudanças significativas nos valores de ΔE (p > 0,05) em qualquer um dos grupos durante os intervalos de acompanhamento (um mês, três meses e seis meses após a aplicação do tratamento). Conclusão: A infiltração de resina é capaz de melhorar a coloração das LMBs e restaurar a aparência natural do esmalte de forma superior à pasta de dentes com Nano-HA e à microabrasão.
Descritores: Cárie Dentária
-Resinas Sintéticas
Espectrofotometria
Cor
Esmalte Dentário
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1278455
Autor: Santos, Eder José dos; Sabatke, Mateus; Herrmann, Amanda Beatriz; Sturgeon, Ralph Edward.
Título: Evaluation of Sample Preparation Procedures for Determination of Cr(VI) in Cr2O3 Pigments by Vis Spectrophotometry
Fonte: Braz. arch. biol. technol;64(spe):e21200455, 2021. tab, graf.
Idioma: en.
Projeto: Fundação Araucária.
Resumo: Abstract Six sample preparation procedures were evaluated for selective extraction of Cr(VI) from commercial samples of chromium oxide green (Cr2O3) pigments prior to formation of its diphenylcarbazone complex [CrDPCO]- for determination by visible spectrophotometry: (I) water-soluble chromium; (II) EPA method 3060A without Mg2+; (III) EPA method 3060A with Mg2+; (IV) Na3PO4 based extraction; (V) method IRSA16 based on acidic extraction and; (VI) Na2CO3 based extraction. Evaluation of the influence of concomitant Cr(III) ions, time and stability of the [CrDPCO]- complex was investigated. Recoveries of soluble and insoluble Cr(VI) species were 86% and 80%, respectively, using procedure (VI). Direct calibration against aqueous standards prepared in the extraction medium was successful for Cr(VI) in the concentration range 0.05-1.50 μg L-1. Limits of detection and quantitation were 0.3 µg g-1 and 1.0 µg g-1, respectively, for 250 mg subsamples/25 mL. Procedure (VI) was applied to the analysis of four commercial samples of Cr2O3 pigments, three determined to have Cr(VI) within compliance limits below 1.0 µg g-1, but one at 16.6 ± 0.6 µg g-1, prohibiting use of this pigment in cosmetic formulations. This sample was conveniently employed to evaluate the accuracy of the method. The recommended procedure is simple and accurate and has been adopted by Tecpar's laboratory of Parana Institute of Technology (Curitiba, Brazil).
Descritores: Pigmentos Biológicos
Espectrofotometria/instrumentação
Cromo/análise
-Brasil
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1149035
Autor: Ponce de León, Patricia; López Murúa, Gabriel.
Título: Trichinella spiralis y Trichinella patagoniensis: estudio de la carga globular aplicando el método de Azul Alcian / Trichinella spiralis and Trichinella patagoniensis: study of globular charge applying Alcian Blue method / Trichinella spiralis e Trichinella patagoniensis: estudo da carga globular pelo método de Azul de Alcian
Fonte: Acta bioquím. clín. latinoam;54(4):461-466, jul. 2020. tab, graf.
Idioma: es.
Resumo: Resumen Los residuos de ácido siálico de las glicoproteínas de superficie son los principales responsables de la carga negativa eritrocitaria. El objetivo de este trabajo fue estudiar alteraciones de carga globular producidas por Trichinella spiralis y Trichinella patagoniensis. Se trabajó con concentrados de larvas musculares de ambas especies y con eritrocitos frescos. Se incubó el sedimento globular con igual volumen de concentrado larval (1 y 2 horas). Los controles fueron incubados con solución salina. Se aplicó el método de Azul Alcian y se determinó el coeficiente experimental de carga aniónica eritrocitaria (CexpCAE). Los resultados mostraron que la carga disminuyó con el aumento del tiempo de tratamiento para ambas especies. Los valores de CexpCAE de las suspensiones incubadas con T. spiralis fueron menores que con T. patagoniensis, indicando que T. spiralis produjo mayor disminución de carga que T. patagoniensis. Se concluye que la desialización producida por ambas especies no es la misma, lo que sugiere que la relación hospedador-parásito que se establecería in vivo sería distinta.

Abstract The sialic acid residues of the surface glycoproteins are mainly responsible for the erythrocyte negative charge. The objective of this work was to study alterations of globular charge produced by Trichinella spiralis and Trichinella patagoniensis. Work was carried out on muscle larvae concentrates of both species and fresh erythrocytes. The treatment was performed by incubating the globular pellet with equal volume of larval concentrate (1 and 2 hours). Controls were incubated with saline solution. The Alcian Blue method was applied and the experimental coefficient of erythrocyte anion charge (expCEAC) was determined. The results showed that the globular charge decreased with increasing treatment time for both species. The expCEAC values of the suspensions incubated with T. spiralis were lower than with T. patagoniensis, indicating that T. spiralis produced a greater decrease in charge than T. patagoniensis. It is concluded that the desialization produced by both species is not the same, suggesting that the host-parasite relationship that would be established in vivo would be different.

Resumo Os resíduos de ácido siálico das glicoproteínas de superfície são os principais responsáveis pela carga negativa dos eritrócitos. O objetivo desse trabalho foi estudar as alterações da carga globular produzidas por Trichinella spiralis e Trichinella patagoniensis. Trabalhamos com concentrados de larvas musculares de ambas as espécies e com eritrócitos frescos. O tratamento foi realizado incubando o sedimento globular com igual volume de concentrado larval (1 e 2 horas), Os controles foram incubados com solução salina. Foi aplicado o método de Azul de Alcian e se determinou o coeficiente experimental de carga aniônica de eritrócitos (CexpCAE). Os resultados mostraram que a carga diminuiu com o aumento do tempo de tratamento para ambas as espécies. Os valores de CexpCAE das suspensões incubadas com T. spiralis foram menores que com T. patagoniensis, indicando que T. spiralis produziu uma diminuição maior na carga que T. patagoniensis. Conclui-se que a dessalinização produzida por ambas as espécies não é a mesma, sugerindo que a relação hospedeiro parasita que seria estabelecida in vivo é diferente.
Descritores: Trichinella/fisiologia
Azul Alciano
Corantes
Eritrócitos/parasitologia
Larva/fisiologia
-Espectrofotometria
Trichinella spiralis/fisiologia
Eritrócitos/química
Interações Hospedeiro-Parasita
Limites: Animais
Responsável: AR144.1 - CIBCHACO - Centro de Información Biomedica del Chaco


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Id: biblio-1056885
Autor: Bhattacharya, Swastika; Purayil, Tina Puthen; Ginjupalli, Kishore; Kini, Sandya; Pai, Swathi.
Título: Effect of Thermocycling on the Colour Stability of Aesthetic Restorative Materials: An in-vitro Spectrophotometric Analysis
Fonte: Pesqui. bras. odontopediatria clín. integr;20:e5174, 2020. tab.
Idioma: en.
Resumo: Abstract Objective: To compare the color stability of Cention N, Fuji IX GP Extra, and Fuji IX GP after thermocycling. Material and Methods: Ten discs of each material of dimension 10 x 1 mm were prepared using a split mold. The preparations of the specimens were done according to the powder/liquid ratio as recommended by the manufacturers [4.6:1, 3.4:1 and 3.6:1 for the groups I, II, and III, respectively]. After setting, the samples were retrieved, and the thickness of each specimen was measured using a micrometer at five different locations. The specimens with variations in thickness, porosity or cracks were discarded and thus not included in the study. The selected specimens were stored in distilled water for 24 hours prior to testing. The prepared specimens were thermocycled at 5°C and 55°C, with a dwell time of 15 seconds for 250 or 500 cycles. Subsequently, the color parameters of the discs were measured using a spectrophotometer. The data were analyzed using two way ANOVA test, and a p-value <0.001 was considered. Results: Thermocycling resulted in changes in the color of both Glass Ionomer cement and Cention N (p<0.001). Among the materials tested, Cention N showed superior color stability. Conclusion: Cention N exhibited better color stability compared to Glass ionomer cements.
Descritores: Espectrofotometria/métodos
Materiais Dentários
Estética Dentária
Cimentos de Ionômeros de Vidro
-Análise de Variância
Índia
Tipo de Publ: Estudo Comparativo
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


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Id: biblio-840833
Autor: Benli, Erdal; Ayyildiz, Sema Nur; Cirrik, Selma; Koktürk, Sibel; Cirakoglu, Abdullah; Noyan, Tevfik; Ayyildiz, Ali; Germiyanoglu, Cankon.
Título: The effect of tadalafil therapy on kidney damage caused by sepsis in a polymicrobial septic model induced in rats: a biochemical and histopathological study
Fonte: Int. braz. j. urol;43(2):345-355, Mar.-Apr. 2017. tab, graf.
Idioma: en.
Projeto: Ordu University Scientific Research Projects Coordination Department.
Resumo: ABSTRACT Introduction Sepsis is an inflammatory reaction to bacteria involving the whole body and is a significant cause of mortality and economic costs. The purpose of this research was to determine whether tadalafil exhibits a preventive effect on sepsis in a septic model induced in rats with cecal ligation and puncture (CLP). Materials and Methods Rats were randomly separated into groups, 10 rats in each: (i) a sham (control) group, (ii) an untreated sepsis group, (iii) a sepsis group treated with 5mg/kg tadalafil and (iv) a sepsis group treated with 10mg/kg tadalafil. A polymicrobial sepsis model was induced in rats using CLP. Rats were sacrificed after 16h, and blood and kidney tissues were collected for biochemical and histopathological study. Results Levels of the inflammatory parameter IL-6 decreased significantly in the sepsis groups receiving tadalafil in comparison with the untreated sepsis group (p<0.05). In terms of histopathology, inflammation scores investigated in kidney tissues decreased significantly in the sepsis groups receiving tadalafil compared to the untreated sepsis group (p<0.05). In addition, levels of creatinine and cystatin C measured in septic rats receiving tadalafil were lower by a clear degree than in septic rats (p<0.05). Conclusion In this study, tadalafil exhibited a preventive effect for sepsis-related damage by suppressing inflammation in serum and kidney tissue of septic rats in a polymicrobial sepsis model induced with CLP.
Descritores: Sepse/complicações
Sepse/prevenção & controle
Insuficiência Renal/etiologia
Insuficiência Renal/prevenção & controle
Inibidores da Fosfodiesterase 5/uso terapêutico
Tadalafila/uso terapêutico
-Valores de Referência
Espectrofotometria
Superóxido Dismutase/análise
Calcitonina/sangue
Ensaio de Imunoadsorção Enzimática
Imuno-Histoquímica
Catalase/análise
Distribuição Aleatória
Reprodutibilidade dos Testes
Interleucina-6/sangue
Ratos Wistar
Peroxidase/análise
Sepse/patologia
Creatinina/sangue
Modelos Animais de Doenças
Insuficiência Renal/patologia
Cistatina C/sangue
Rim/efeitos dos fármacos
Rim/patologia
Ligadura
Malondialdeído/análise
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME



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