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Id: biblio-829668
Autor: Porto, Karla Rejane de Andrade; Motti, Priscilla Rezende; Machado, Alexandre Alves; Roel, Antonia Railda.
Título: In vitro evaluation of the effect of botanical formulations used in the control of Aedes aegypti L. (Diptera: Culicidae) on liver enzymes.
Fonte: Rev. Soc. Bras. Med. Trop;49(6):693-697, Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract: INTRODUCTION: Dengue fever is a viral disease transmitted by the Aedes aegypti Linn. (1792) (Diptera: Culicidae) mosquito, which is endemic in several regions of Brazil. Alternative methods for the control of the vector include botanical insecticides, which offer advantages such as lower environmental contamination levels and less likelihood of resistant populations. Thus, in this study, the ability of botanical insecticide formulations to inhibit the activity of the liver enzymes serum cholinesterase and malate dehydrogenase was evaluated. METHODS: Inhibition profiles were assessed using in vitro assays for cholinesterase and malate dehydrogenase activity and quantitated by ultraviolet-visible spectroscopy at 410nm to 340nm. RESULTS Insecticide products formulated from cashew nutshell liquid [A] and ricinoleic acid [B] showed cholinesterase activity levels of 6.26IU/mL and 6.61IU/mL, respectively, while the control level for cholinesterase was 5-12IU/mL. The products did not affect the level of 0.44IU/mL established for malate dehydrogenase, as the levels produced by [A] and [B] were 0.43IU/mL and 0.45IU/mL, respectively. CONCLUSIONS Our findings show that in vitro testing of the formulated products at concentrations lethal to A. aegypti did not affect the activity of cholinesterase and malate dehydrogenase, indicating the safety of these products.
Descritores: Ácidos Ricinoleicos/farmacologia
Inibidores da Colinesterase/farmacologia
Colinesterases/efeitos dos fármacos
Anacardium/química
Inseticidas/farmacologia
Fígado/enzimologia
Malato Desidrogenase/antagonistas & inibidores
-Espectrofotometria Ultravioleta
Técnicas In Vitro
Ácidos Ricinoleicos/isolamento & purificação
Aedes
Insetos Vetores/efeitos dos fármacos
Inseticidas/isolamento & purificação
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME


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Id: biblio-897000
Autor: Carmo, Ana Paula Barbosa do; Borborema, Manoella; Ribeiro, Stephan; De-Oliveira, Ana Cecilia Xavier; Paumgartten, Francisco Jose Roma; Moreira, Davyson de Lima.
Título: A newly validated high-performance liquid chromatography method with diode array ultraviolet detection for analysis of the antimalarial drug primaquine in the blood plasma
Fonte: Rev. Soc. Bras. Med. Trop;50(4):499-505, July-Aug. 2017. tab, graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: Primaquine (PQ) diphosphate is an 8-aminoquinoline antimalarial drug with unique therapeutic properties. It is the only drug that prevents relapses of Plasmodium vivax or Plasmodium ovale infections. In this study, a fast, sensitive, cost-effective, and robust method for the extraction and high-performance liquid chromatography with diode array ultraviolet detection (HPLC-DAD-UV ) analysis of PQ in the blood plasma was developed and validated. METHODS: After plasma protein precipitation, PQ was obtained by liquid-liquid extraction and analyzed by HPLC-DAD-UV with a modified-silica cyanopropyl column (250mm × 4.6mm i.d. × 5μm) as the stationary phase and a mixture of acetonitrile and 10mM ammonium acetate buffer (pH = 3.80) (45:55) as the mobile phase. The flow rate was 1.0mL·min-1, the oven temperature was 50OC, and absorbance was measured at 264nm. The method was validated for linearity, intra-day and inter-day precision, accuracy, recovery, and robustness. The detection (LOD) and quantification (LOQ) limits were 1.0 and 3.5ng·mL-1, respectively. The method was used to analyze the plasma of female DBA-2 mice treated with 20mg.kg-1 (oral) PQ diphosphate. RESULTS: By combining a simple, low-cost extraction procedure with a sensitive, precise, accurate, and robust method, it was possible to analyze PQ in small volumes of plasma. The new method presents lower LOD and LOQ limits and requires a shorter analysis time and smaller plasma volumes than those of previously reported HPLC methods with DAD-UV detection. CONCLUSIONS: The new validated method is suitable for kinetic studies of PQ in small rodents, including mouse models for the study of malaria.
Descritores: Primaquina/sangue
Antimaláricos/sangue
-Primaquina/farmacocinética
Espectrofotometria Ultravioleta
Cromatografia Líquida de Alta Pressão
Camundongos
Antimaláricos/farmacocinética
Limites: Animais
Feminino
Tipo de Publ: Estudo de Validação
Responsável: BR1.1 - BIREME


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Id: biblio-1104201
Autor: Mahmood, Rashid; Malik, Farnaz; Shamas, Shazia; Ahmed, Tanveer; Kausar, Mehran; Rubnawaz, Samina; Ashfaq, Muhammad; Hussain, Shahzad; Green, Brian Desmond; Mirza, Bushra.
Título: Pharmacological evaluation of Rhazya stricta root extract / Evaluación farmacológica del extracto de raíz de Rhazya stricta
Fonte: Bol. latinoam. Caribe plantas med. aromát;19(2):188-206, mar. 2020. ilus, tab.
Idioma: en.
Resumo: The present study aimed to screen the Rhazya stricta Decne root for its antihyperglycemic and antioxidants potential through invitro assays along with phytochemical and elemental analyses. The crude extract was prepared through maceration and fractionated using solvent-solvent extraction technique. The spectroscopic studies indicated the presence of various phytochemical classes in the extract and its fractions. The antioxidant assays showed notable results along with a good concentration of phenolic and flavonoid contents. Enzyme inhibition assays demonstrated glucose-lowering effects by inhibiting the enzyme activity which could reduce post-prandial blood glucose level. The Dipeptidyl peptidase-IV (DPP-IV) inhibition assay results showed the novel DPP-IV inhibition activity of the plant extract and all fractions showed noteworthy enzyme inhibition and antihyperglycemic activity. Conclusively, the Rhazya stricta root extract displayed its antioxidant and antihyperglycemic potential due to the presence of various classes of phytochemicals and micro-nutrients.

El presente estudio tuvo como objetivo examinar la raíz de Rhazya stricta Decne por su potencial antihiperglicémico y antioxidante a través de ensayos in vitro junto con análisis fitoquímicos y elementales. El extracto crudo se preparó por maceración y se fraccionó usando una técnica de extracción solvente-solvente. Los estudios espectroscópicos indicaron la presencia de varias clases fitoquímicas en el extracto y sus fracciones. Los ensayos antioxidantes mostraron resultados notables junto con una importante concentración de contenido fenólico y flavonoide. Los ensayos de inhibición enzimática demostraron efectos reductores de la glucosa al inhibir la actividad enzimática que podría reducir el nivel de glucosa posprandial en sangre. Los resultados del ensayo de inhibición de Dipeptidyl peptidase-IV (DPP-IV) mostraron la nueva actividad de inhibición de DPP-IV del extracto de la planta y todas las fracciones mostraron una notable inhibición enzimática y actividad antihiperglicémica. En conclusión, el extracto de raíz de Rhazya stricta Decne mostró su potencial antioxidante y antihiperglicémico debido a la presencia de varias clases de fitoquímicos y micronutrientes.
Descritores: Extratos Vegetais/farmacologia
Apocynaceae/química
Hipoglicemiantes/farmacologia
Antioxidantes/farmacologia
-Fenóis/análise
Espectrofotometria Ultravioleta
Flavonoides/análise
Glicemia/efeitos dos fármacos
Técnicas In Vitro
Extratos Vegetais/química
Espectroscopia de Infravermelho com Transformada de Fourier
Raízes de Plantas/química
Dipeptidil Peptidases e Tripeptidil Peptidases/antagonistas & inibidores
Compostos Fitoquímicos
Hipoglicemiantes/química
Antioxidantes/química
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-839184
Autor: Campana, Eloiza Helena; Xavier, Danilo Elias; Petrolini, Fernanda Villas-Boas; Cordeiro-Moura, Jhonatha Rodrigo; Araujo, Maria Rita Elmor de; Gales, Ana Cristina.
Título: Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil
Fonte: Braz. j. infect. dis;21(1):57-62, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: Abstract The mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in São Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. β-lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays. The mRNA transcription level of oprD was assessed by qRT-PCR and the outer membrane proteins profile was evaluated by SDS-PAGE. Genetic relationship among P. aeruginosa isolates was assessed by PFGE. Carbapenems hydrolysis was not detected by carbapenemase assay in the carbapenem-resistant and cephalosporin-susceptible P. aueruginosa clinical isolates. OprD decreased expression was observed in all P. aeruginosa isolates by qRT-PCR. The outer membrane protein profile by SDS-PAGE suggested a change in the expression of the 46 kDa porin that could correspond to OprD porin. The isolates were clustered into 17 genotypes without predominance of a specific PFGE pattern. These results emphasize the involvement of multiple chromosomal mechanisms in carbapenem-resistance among clinical isolates of P. aeruginosa, alert for adaptation of P. aeruginosa clinical isolates under antimicrobial selective pressure and make aware of the emergence of an uncommon phenotype among P. aeruginosa clinical isolates.
Descritores: Pseudomonas aeruginosa/efeitos dos fármacos
Carbapenêmicos/farmacologia
Cefalosporinas/farmacologia
Resistência beta-Lactâmica/genética
Antibacterianos/farmacologia
-Fenótipo
Pseudomonas aeruginosa/enzimologia
Pseudomonas aeruginosa/genética
Espectrofotometria Ultravioleta
Proteínas da Membrana Bacteriana Externa
Proteínas de Bactérias/metabolismo
beta-Lactamases/metabolismo
Brasil
DNA Bacteriano
Testes de Sensibilidade Microbiana
Eletroforese em Gel de Campo Pulsado
Análise de Sequência de DNA
Porinas/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-731955
Autor: Pérez Navarro, Maikel; Rodríguez Hernández, Yaslenis; Suárez Pérez, Yania.
Título: Validación del método por espectrofotometría ultravioleta para control de calidad de clorhidrato de ciprofloxacina en tabletas Ciprecu / Validation of the ultraviolet spectrophotometry method for the quality control of ciprofloxacin chlorhydrate in Ciprecu tablets
Fonte: Rev. cuba. farm;48(2), abr.-jun. 2014. Ilus, tab.
Idioma: es.
Resumo: INTRODUCCCIÓN: las quinolonas son un grupo de agentes antimicrobianos de gran importancia en la clínica. El clorhidrato de ciprofloxacina monohidrato es una fluoroquinolona antibacterial de segunda generación que se indica en el tratamiento de diversas infecciones y se comercializa en forma de colirio, inyectable, cápsulas y tabletas. OBJETIVO: desarrollar y validar un método analítico por espectrofotometría ultravioleta, con vistas a su aplicación al control de calidad del clorhidrato de ciprofloxacina en tabletas Ciprecu recién elaboradas. MÉTODOS: se desarrolló el método en el laboratorio y se realizó una validación exhaustiva atendiendo a los parámetros de la categoría I. El método se seleccionó teniendo en cuenta la presencia de grupos cromóforos en la estructura del compuesto analizado. Se determinó la longitud de onda de máxima absorción a 273 nm de 5 µg/mL en ácido clorhídrico 0,1 mol/L. RESULTADOS: a partir del proceso de validación realizado, se demostró la adecuada especificidad frente a los componentes de la matriz en estudio, así como su linealidad, exactitud y precisión en el rango de 2,5 a 7,5 µg/mL. Los resultados de la aplicación de este método fueron similares a los obtenidos por el método oficial propuesto con iguales propósitos en USP 33, 2010. CONCLUSIONES: el método fue válido con el objetivo propuesto, lo cual constituye una nueva alternativa simple, rápida y económica para el control de calidad de clorhidrato de ciprofloxacina en tabletas Ciprecu(AU)

INTRODUCTION: quinolones are a group of antimicrobials of high clinical significance. Ciprofloxacin hydrochloride monohydrate is a second-generation antibacterial fluoroquinolone for treatment of several infections and is marketed as eye drops, injections, capsule and tablets. OBJECTIVE: to develop and to validate an ultraviolet spectrophotometric analytical method to be used in the quality control of ciprofloxacin hydrochloride monohydrate in newly manufactured Ciprecu tablets. METHODS: this method was devised at the laboratory and thoroughly validated pursuant to the category I parameters. The method was selected on account of the existence of chromophore groups in the structure of the analyzed compound. The maximum absorption wavelength was set at 273 nm of 5 µg/mL in 0.1 mol/L hydrochloric acid. RESULTS: based on the validation process, it was demonstrated that this method has adequate specificity against the study matrix components, as well as its linearity, accuracy and precision in the range of 2.5 to 7.5 µg/mL. The results of the application of this method were similar to those of the official procedure suggested for the same purposes in USP 33, 2010. CONCLUSIONS: The analytical method was valid for the suggested purposes, so it is a new simple, rapid and economic alternative for the quality control of Ciprofloxacin hydrochloride in Ciprecu tablets(AU)
Descritores: Controle de Qualidade
Espectrofotometria Ultravioleta/métodos
Ciprofloxacina/uso terapêutico
-Comprimidos
Estudos de Validação como Assunto
Limites: Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: biblio-1007989
Autor: Paredes, Maria E; Morales, Rita E; Lima, Wendi K; Hu, Siyao; Jurado, Ana LV; Farchi, Eugenia; Prieto, Jose M.
Título: Morphoanatomical and phytochemical studies for the quality control of Neurolaena lobata (L.) R.Br. ex Cass. (Asteraceae) / Estudios morfoanatomicos y fitoquimicos para el control de calidad de Neurolaena lobata (L.) R.Br. ex Cass. (Asteraceae)
Fonte: Bol. latinoam. Caribe plantas med. aromát;18(3):277-288, mayo 2019. ilus.
Idioma: en.
Resumo: Neurolaena lobata (L.) R.Br. ex Cass. (Asteraceae)Is a popular folk remedy for in Central America. The plant is of commercial value in Guatemala but so far there is not any monograph to guide regional laboratories on ensuring identity and chemical tests for this species. As identity test we here run macro and micro morphoanatomical studies of the characters of the vegetative organs. We also developed standard chemical tests for quality by both TLC and HPLC for infusions and tinctures of varying alcoholic strength. Their radical scavenging activities in DPPH and NO were also measured. Macro and micro morphoanatomical characters of the vegetative organs present a set of characteristics to facilitate the identification of dry powdered samples of this species. We developed optimal conditions for the TLC and HPLC phytochemical fingerprints of the 4 most common pharmacopoeial liquid herbal preparations from this herbal drug, namely infusion, 70%, 45% and 20% hydroalcoholic tinctures. Our work provides the Latin-American industry with a set of analyses to establish the identity and chemistry of N. lobata samples for quality control purposes.

Neurolaena lobata (L.) R.Br. ex cass. (Asteraceae) es un remedio popular popular en América Central. La planta tiene un valor comercial en Guatemala, pero hasta el momento no existe una monografía que guíe a los laboratorios regionales para garantizar la identidad y las pruebas químicas para esta especie. Como prueba de identidad proponemos estudios macro y micro morfoanatómicos de los caracteres de los órganos vegetativos. También desarrollamos pruebas químicas de calidad mediante CCF y CLAR para infusiones y tinturas de grado alcohólico variable. También se midieron sus actividades de captación de radicales en DPPH y NO. Los caracteres macro y micro morfoanatómicos de los órganos vegetativos presentan un conjunto de características para facilitar la identificación de muestras de polvo seco de esta especie. Desarrollamos condiciones óptimas para las huellas dactilares fitoquímicas de CCF y CLAR de las 4 preparaciones herbales líquidas farmacopéicas más comunes de esta droga herbal, a saber, infusión, 70%, 45% y 20% tinturas hidroalcohólicas. Nuestro trabajo proporciona a la industria latinoamericana un conjunto de análisis base para establecer la identidad y la química de las muestras de N. lobata con fines de control de calidad.
Descritores: Asteraceae/anatomia & histologia
Asteraceae/química
Compostos Fitoquímicos/análise
-Controle de Qualidade
Espectrofotometria Ultravioleta
Sequestradores de Radicais Livres
Cromatografia Líquida de Alta Pressão
Cromatografia em Camada Delgada
Asteraceae/ultraestrutura
Guatemala
Microscopia
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-844862
Autor: Pérez Navarro, Maikel; Rodríguez Hernández, Yasleny; Suárez Pérez, Yania.
Título: Validación del método espectrofotométrico para control de calidad de la colchicina en Artrichine tabletas / Validation of the spectrophotometric method for the quality control of Artrichine tablets
Fonte: Rev. cuba. farm;50(1), ene.-mar. 2016. ilus, graf, tab.
Idioma: es.
Resumo: Introducción: la colchicina es una alternativa terapéutica indicada por vía oral para las crisis agudas de la gota. Se formula en tabletas de baja dosificación debido a su elevada toxicidad. Los Laboratorios Dr.A. Bjarner C.A producen las tabletas de Artrichine y se requiere de un método sencillo, pero a la vez confiable para realizar el control de calidad de este producto terminado, que considere la solubilidad en etanol, la presencia de cromóforos y la composición de la formulación de la colchicina. Objetivo: se propuso validar un método por espectrofotomería UV útil para el control de rutina. Métodos: se aplicó un método simple, que se modifica del método establecido en la Farmacopea Británica del 2009 para las tabletas, por espectrofotometría UV directa. Se basa en la extracción del analito en etanol absoluto y su posterior determinación a 350 nm. La validación del método se realizó a través de los parámetros linealidad, precisión, exactitud y especificidad frente a los componentes de la formulación. Resultados: se estableció una metodología analítica muy sencilla para obtener una solución transparente a partir de la forma terminada, de igual concentración a la solución de referencia. El cumplimiento satisfactorio de todos los criterios de aceptación establecidos para los parámetros evaluados permitió demostrar la validez del método en estudio para el control de calidad en el rango de 50 a 150 por ciento (5-15 µg/mL). Conclusiones: el método por espectrofotometría UV resultó específico, lineal, exacto y preciso para su aplicación al control de calidad de la colchicina en Artrichine tabletas(AU)

Introduction: colchicine is a therapeutic alternative orally prescribed for acute gout. It is formulated as low dose tables due to its high toxicity. Dr A. Bjarner C.A laboratories manufacture Artrichine tablets and it requires a simple and reliable method to conduct quality control of the finished product that will consider ethanol-soluble characteristics, presence of chromophores and composition of colchicine formulation. Objective: to validate an ultraviolet spectrophotometry-based method for the routine quality control. Methods: a simple method by direct UV spectrophotometry which is modified from the set method of the British Pharmacopeia 2009 for tablets. It is based on the analyte extraction in absolute ethanol and the estimation at 350 nm. The method was validated on account of linearity, precision, accuracy and specificity against the formulation components. Results: a very simple analytical methodology was established to obtain a transparent solution from the finished form, with the same concentration as that of the reference solution. The satisfactory compliance with all the acceptance criteria for the evaluated parameters allowed proving the validity of the study method for the quality control in the 50 to 150 percent range (5-15 ug/ml). Conclusions: the UV spectrophotometry-based method proved to be specific, linear, accurate and precise for the quality control of colchicine in Artrichine tablets(AU)
Descritores: Colchicina/uso terapêutico
Medicamentos de Referência
Estudos de Validação como Assunto
Gota/terapia
-Espectrofotometria Ultravioleta/métodos
Limites: Humanos
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: lil-432162
Autor: Mazon, Elaine Marra de Azevedo; Oliveira, Aline Cristine Garcia de; Brígido, Berenice Mandel; Freitas, Valéria Pereira da Silva.
Título: Estudo comparativo de métodos para determinação de nitrato em águas para consumo humano / Comparative study on nitrate determination methods in potable water
Fonte: Rev. Inst. Adolfo Lutz;64(1):110-116, jan.-jun. 2005. tab.
Idioma: pt.
Resumo: Foi realizado um estudo comparativo entre quatro métodos para determinação de nitrato em águas destinadas ao consumo humano: ácido fenoldissulfônico, espectrofotométrico UV a 205nm, espectrofotométrico UV a 205nm modificado e espectrofotométrico UV a 220/275nm. Os métodos foram avaliados a fim de selecionar os mais adequados quanto à precisão e exatidão para serem aplicados na rede oficial de laboratórios de saúde pública. Com base nos resultados obtidos, os métodos espectrofotométricos UV a 220/275nm e espectrofotométrico UV a 205nm apresentaram características de desempenho adequadas estatisticamente para análise de rotina laboratorial, além de serem métodos de fácil execução, utilizarem reagente único e apresentarem resultados imediatos na determinação de nitratos em águas com baixo teor de matéria orgânica
Descritores: Nitratos
Água Potável
-Espectrofotometria Ultravioleta
Tipo de Publ: Estudo Comparativo
Responsável: BR91.2 - Centro de Documentação


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Raw, Isaias
Id: lil-263418
Autor: Iourtov, Dmitri; Kubrusly, Flávia S; Tenório, Elisabeth C. N; Higashi, Hisako G; Raw, Isaias.
Título: Tween 20, Brij 35 and Triton X-100 determination exploiting the properties of the oxyethylene groups in the presence of proteins
Fonte: RBCF, Rev. bras. ciênc. farm. (Impr.);35(2):245-9, jul.-dez. 1999. ilus, tab, graf.
Idioma: en.
Resumo: A determinação espectrofotométrica na região do ultravioleta para quantificação de detergentes foi baseada na formação de complexo não estequiométrico entre o detergente e os reagentes (cloreto de bário e ácido fosfomolíbdico). A quantidade de complexo dissolvido com 2-metoxietanol foi quantitativamente medida em 310 nm. A metodologia foi reprodutível na faixa de 1-50 µg/mL para os três detergentes testados: Tween 20, Brij 35 e Triton X-100. Detectou-se interferência da proteína. Para superar tal interferência, brancos diferentes foram usados para as curvas padrão dos detergentes e para as amostras de proteínas purificadas
Descritores: Polissorbatos/análise
Detergentes
Proteínas
-Espectrofotometria Ultravioleta
Indicadores e Reagentes
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


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Texto completo SciELO Brasil
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Id: biblio-839459
Autor: Silva, Taízia Dutra; Toledo, Cibele Rodrigues; Vianna-Soares, Cristina Duarte.
Título: Development and validation of alternative methods by non-aqueous acid-base titration and derivative ultraviolet spectrophotometry for quantification of sildenafil in raw material and tablets
Fonte: Braz. J. Pharm. Sci. (Online);53(1):e15181, 2017. tab, graf.
Idioma: en.
Resumo: Sildenafil citrate (SILC) is a potent phosphodiesterase-5 inhibitor used for erectile dysfunction and pulmonary hypertension. This study shows two simple, fast and alternative analytical methods for SILC determination by non-aqueous titration and by derivative ultraviolet spectrophotometry (DUS) in active pharmaceutical ingredient and/or dosage forms. The quantitation method of SILC active pharmaceutical ingredient by non-aqueous acid-base titration was developed using methanol as solvent and 0.1 mol/L of perchloric acid in acetic acid as titrant. The endpoint was potentiometrically detected. The non-aqueous titration method shows satisfactory repeatability and intermediate precision (RSD 0.70-1.09%). The neutralization reaction occurred in the stoichiometric ratio 1:1 in methanol. The determination of SILC active pharmaceutical ingredient or dosage forms by DUS was developed in the linear range from 10 to 40 µg/mL, in 0.01 mol/L HCl, using the first order zero-peak method at λ 256 nm. The DUS method shows selectivity toward tablets excipients, appropriate linearity (R2 0.9996), trueness (recovery range 98.86-99.30%), repeatability and intermediate precision in three concentration levels (RSD 1.17-1.28%; 1.29-1.71%, respectively). Therefore, the methods developed are excellent alternatives to sophisticated instrumental methods and can be easily applied in any pharmaceutical laboratory routine due to simple and fast executions.
Descritores: Espectrofotometria Ultravioleta/métodos
Titulometria/métodos
Citrato de Sildenafila/análise
-Comprimidos/farmacologia
Vasodilatadores/classificação
Tipo de Publ: Estudo de Validação
Responsável: BR1.1 - BIREME



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