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Id: biblio-1117007
Autor: Vaccaro, Carlos A; González, Marcos; Peralta, Nadia C.
Título: Medicina de precisión: aspectos prácticos para el cirujano moderno / Precision medicine: practical aspects for the modern surgeon
Fonte: Rev. argent. coloproctología;31(2):51-53, jun. 2020.
Idioma: en; es.
Resumo: Los contenidos de este capítulo se basan en la 3a edición de las Clínicas Quirúrgicas de Cáncer Colorrectal. C. Vaccaro y N. Peralta. del hospital ediciones 2020 (en prensa)
Descritores: Neoplasias Colorretais/genética
Biomarcadores Tumorais/genética
Medicina de Precisão/tendências
-Farmacogenética/tendências
Neoplasias Colorretais/etiologia
Neoplasias Colorretais/terapia
Epidemiologia Molecular/tendências
Mutação
Metástase Neoplásica/genética
Metástase Neoplásica/terapia
Responsável: AR1.1 - Biblioteca Rafael Herrera Vegas


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Elucir, Gir
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Id: lil-776534
Autor: Okado, Jessica Baleiro; Bogni, Simoni Camila; Reinato, Lílian Andreia Fleck; Martinez, Roberto; Gir, Elucir; Camargo, Ilana Lopes Baratella da Cunha.
Título: Molecular analysis of methicillin-resistant Staphylococcus aureus dissemination among healthcare professionals and/or HIV patients from a tertiary hospital
Fonte: Rev. Soc. Bras. Med. Trop;49(1):51-56, Jan.-Feb. 2016. graf.
Idioma: en.
Projeto: São Paulo Research Foundation; . Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); . Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Resumo: Abstract INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is a nosocomial pathogen in community settings. MRSA colonized individuals may contribute to its dissemination; the risk of MRSA infection is increased in human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS) patients, although the prevalence of colonization in this group is not well established. The present study addressed this issue by characterizing MRSA isolates from HIV/AIDS patients and their healthcare providers (HCPs) to determine whether transmission occurred between these two populations. METHODS: A total of 24 MRSA isolates from HIV-infected patients and five from HCPs were collected between August 2011 and May 2013. Susceptibility to currently available antimicrobials was determined. Epidemiological typing was carried out by pulsed-field gel electrophoresis, multilocus sequence typing, and Staphylococcus cassette chromosome (SCCmec) typing. The presence of heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) and heterogeneous daptomycin-resistant Staphylococcus aureus (hDRSA) was confirmed by population analysis profile. Isolates characterized in this study were also compared to isolates from 2009 obtained from patients at the same hospital. RESULTS: A variety of lineages were found among patients, including ST5-SCCmecII and ST30-SCCmecIV. Two isolates were Panton-Valentine leukocidin-positive, and hVISA and hDRSA were detected. MRSA isolates from two HCPs were not related to those from HIV/AIDS patients, but clustered with archived MRSA from 2009 with no known relationship to the current study population. CONCLUSIONS: ST105-SCCmecII clones that colonized professionals in 2011 and 2012 were already circulating among patients in 2009, but there is no evidence that these clones spread to or between HIV/AIDS patients up to the 7th day of their hospitalization.
Descritores: Infecções Estafilocócicas
Infecções por HIV/microbiologia
Infecção Hospitalar/transmissão
Transmissão de Doença Infecciosa do Profissional para o Paciente/estatística & dados numéricos
Staphylococcus aureus Resistente à Meticilina/genética
Antibacterianos/farmacologia
-Infecções Estafilocócicas/microbiologia
Testes de Sensibilidade Microbiana
Infecção Hospitalar/microbiologia
Técnicas de Tipagem Bacteriana
Eletroforese em Gel de Campo Pulsado
Epidemiologia Molecular
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Tipagem de Sequências Multilocus
Centros de Atenção Terciária
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-782105
Autor: Feyisa, Seifu Gizaw; Haeili, Mehri; Zahednamazi, Fatemeh; Mosavari, Nader; Taheri, Mohammad Mohammad; Hamzehloo, Gholamreza; Zamani, Samin; Feizabadi, Mohammad Mehdi.
Título: Molecular characterization of mycobacterium tuberculosis isolates from Tehran, Iran by restriction fragment length polymorphism analysis and spoligotyping
Fonte: Rev. Soc. Bras. Med. Trop;49(2):204-210, Mar.-Apr. 2016. tab, graf.
Idioma: en.
Projeto: Tehran University of Medical Sciences.
Resumo: Abstract: INTRODUCTION Characterization of Mycobacterium tuberculosis (MTB) isolates by DNA fingerprinting has contributed to tuberculosis (TB) control. The aim of this study was to determine the genetic diversity of MTB isolates from Tehran province in Iran. METHODS MTB isolates from 60 Iranian and 10 Afghan TB patients were fingerprinted by standard IS6110-restriction fragment length polymorphism (RFLP) analysis and spoligotyping. RESULTS The copy number of IS6110 ranged from 10-24 per isolate. The isolates were classified into 22 clusters showing ≥ 80% similarity by RFLP analysis. Fourteen multidrug-resistant (MDR) isolates were grouped into 4 IS6110-RFLP clusters, with 10 isolates [71% (95% CI: 45-89%)] in 1 cluster, suggesting a possible epidemiological linkage. Eighteen Iranian isolates showed ≥ 80% similarity with Afghan isolates. There were no strains with identical fingerprints. Spoligotyping of 70 isolates produced 23 distinct patterns. Sixty (85.7%) isolates were grouped into 13 clusters, while the remaining 10 isolates (14.2%) were not clustered. Ural (formerly Haarlem4) (n = 22, 31.4%) was the most common family followed by Central Asian strain (CAS) (n = 18, 25.7%) and T (n = 9, 12.8%) families. Only 1strain was characterized as having the Beijing genotype. Among 60 Iranian and 10 Afghan MTB isolates, 25% (95% CI: 16-37) and 70% (95% CI: 39-89) were categorized as Ural lineage, respectively. CONCLUSIONS A higher prevalence of Ural family MTB isolates among Afghan patients than among Iranian patients suggests the possible transmission of this lineage following the immigration of Afghans to Iran.
Descritores: Tuberculose/microbiologia
Variação Genética/genética
DNA Bacteriano/genética
Técnicas de Tipagem Bacteriana/métodos
Mycobacterium tuberculosis/genética
-Polimorfismo de Fragmento de Restrição
Análise por Conglomerados
Impressões Digitais de DNA
Epidemiologia Molecular
Genótipo
Irã (Geográfico)
Mycobacterium tuberculosis/isolamento & purificação
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-842823
Autor: Guerra-Gomes, Isabel Cristina; Gois, Bruna Macêdo; Peixoto, Rephany Fonseca; Oliveira, Camila Alves; Maciel, Bruna Leal Lima; Sarmento, Maria Izabel Ferreira; Pachá, Anna Stella Cysneiros; Araújo, Josélio Maria Galvão; Amaral, Ian Porto Gurgel; Keesen, Tatjana Souza Lima.
Título: Molecular and clinical epidemiological surveillance of dengue virus in Paraíba, Northeast Brazil
Fonte: Rev. Soc. Bras. Med. Trop;50(1):19-26, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: ABSTRACT INTRODUCTION: Despite being the most prevalent arboviral disease worldwide, dengue has been neglected lately. However, recent epidemics of arboviruses such as Zika and chikungunya in locations throughout the world have alerted health authorities to these diseases. This study evaluated the incidence pattern of dengue, its clinical characteristics, and co-circulation of serotypes from 2007 to 2015 in Paraiba State, Northeast Brazil. METHODS: Data on dengue cases from 2007 to 2015 were extracted from clinical reports of the National System for Notifiable Diseases [Sistema Nacional de Agravos de Notificação (SINAN)] of Brazil provided by the Paraiba Health Department. Reverse transcription polymerase chain reaction (RT-PCR) assays for dengue serotypes were carried out on plasma samples obtained from patients with suspected dengue. The data were analysed using descriptive statistics. RESULTS: According to clinical features, dengue fever [n = 39,083 (70.2%)] and dengue without warning signs [n = 15,365 (27.7%)] were the most common classifications of dengue. On RT-PCR, DENV 1 was the most commonly identified serotype (80.5%) in all years studied. Co-circulation of all four DENV serotypes was observed in 2013 and 2014. Furthermore, we observed an increase in dengue notifications in 2015, possibly due to the rise of Zika and chikungunya. CONCLUSIONS: Our findings support the hypothesis that co-circulation of the four DENV serotypes may be a reason for the increased prevalence of severe forms of dengue in the years studied. This study may contribute to directing research, health policy, and financial resources toward reducing poorly controlled epidemic diseases.
Descritores: Dengue/epidemiologia
Vírus da Dengue/genética
-Brasil/epidemiologia
Sorotipagem
Incidência
Epidemiologia Molecular
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Dengue/virologia
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Recém-Nascido
Lactente
Pré-Escolar
Criança
Adolescente
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-1013299
Autor: Bride, Lais de Lima; Pereira, Monalessa Fábia; Barbosa, Maralisi Coutinho; Silva, Nayara Carvalho; Klein, Nazareth Magnago; Nascimento, Thiago César; Schuenck, Ricardo Pinto.
Título: Differences in resistance profiles and virulence genes among methicillin-resistant and methicillin-susceptible Staphylococcus aureus of different lineages at a public tertiary hospital
Fonte: Rev. Soc. Bras. Med. Trop;52:e20190095, 2019. tab, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; . Fundação de Amparo à Pesquisa do Estado do Espírito Santo.
Resumo: Abstract INTRODUCTION Staphylococcus aureus is a major nosocomial pathogen that is associated with high virulence and the rapid development of drug resistance. METHODS We analyzed and compared the antimicrobial resistance, virulence profiles, and molecular epidemiology of 67 S. aureus strains, including 36 methicillin-sensitive (MSSA) and 31 methicillin-resistant (MRSA) strains recovered from a public hospital located in south-eastern Brazil. RESULTS The clones circulating in this hospital presented a great diversity, and the majority of the strains were related to clones responsible for causing worldwide epidemics: these included USA100 (New York/Japan clone), USA300, and USA600. The 31 MRSA (22 SCCmecII and 9 SCCmecIV) and 36 MSSA strains exhibited low resistance against gentamicin and trimethoprim/sulfamethoxazole. No MRSA strain showed resistance to tetracycline. Virulence gene carriage was more diverse and abundant in MSSA than in MRSA. Of the evaluated adhesion-related genes, ebpS was the most prevalent in both MSSA and MRSA strains. The genes bbp and cna showed a strong association with MSSA strains. CONCLUSIONS Our findings reinforce the idea that MSSA and MRSA strains should be carefully monitored, owing to their high pathogenic potential.
Descritores: Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/genética
Resistência a Meticilina
Fatores de Virulência/genética
Staphylococcus aureus Resistente à Meticilina/genética
Antibacterianos/farmacologia
-Infecções Estafilocócicas/epidemiologia
Staphylococcus aureus/isolamento & purificação
Staphylococcus aureus/classificação
Virulência/genética
Brasil/epidemiologia
Testes de Sensibilidade Microbiana
Epidemiologia Molecular
Centros de Atenção Terciária
Hospitais Públicos
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1136920
Autor: Ribeiro, Edlainny Araujo; Gales, Ana Cristina; Oliveira, Ana Paula Streling de; Coelho, Danilo Dias; Oliveira, Rodrigo Alves de; Pfrimer, Irmtraut Araci Hoffmann; Carmo Filho, José Rodrigues do.
Título: Molecular epidemiology and drug resistance of Acinetobacter baumannii isolated from a regional hospital in the Brazilian Amazon region
Fonte: Rev. Soc. Bras. Med. Trop;54:e20200087, 2021. tab, graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: In this study, we report a clonal dissemination of carbapenem resistant Acinetobacter baumannii isolates due to the acquisition of blaOXA-23 in a regional hospital located in Brazilian Amazon Region. METHODS: The isolates were identified by MALDI-TOF and the carbapenemase-encoding genes were detected by multiplex-PCR. The genetic similarity was investigated by pulsed-field gel electrophoresis (PFGE). RESULTS: Only 10 (55.6%) isolates harbored the gene bla OXA-23. PFGE analysis revealed that these isolates belong to a single clone. CONCLUSIONS: This dissemination strategy indicates the need for surveillance, adoption of control procedures defined in guidelines, and the careful administration of antimicrobials should be reinforced.
Descritores: Infecções por Acinetobacter/epidemiologia
Acinetobacter baumannii/genética
-Proteínas de Bactérias/genética
beta-Lactamases/genética
Brasil/epidemiologia
Resistência a Medicamentos
Testes de Sensibilidade Microbiana
Eletroforese em Gel de Campo Pulsado
Epidemiologia Molecular
Hospitais
Antibacterianos/farmacologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-780805
Autor: Berber, Ismet; Avsar, Cumhur; Yegin, Zeynep; Tekerci, Melike; Civek, Seyhan.
Título: Molecular epidemiology of Pseudomonas aeruginosa clinical isolates
Fonte: Braz. j. infect. dis;20(2):224-225, Mar.-Apr. 2016. graf.
Idioma: en.
Descritores: Pseudomonas aeruginosa/genética
Fatores de Virulência/genética
Antibacterianos/farmacologia
-Pseudomonas aeruginosa/efeitos dos fármacos
Turquia
DNA Bacteriano/análise
Epidemiologia Molecular
Genes Bacterianos/genética
Limites: Humanos
Tipo de Publ: Carta
Responsável: BR1.1 - BIREME


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Id: biblio-1039219
Autor: Wirth, Fernanda; Azevedo, Maria Isabel; Goldani, Luciano Z.
Título: Molecular types of Cryptococcus species isolated from patients with cryptococcal meningitis in a Brazilian tertiary care hospital
Fonte: Braz. j. infect. dis;22(6):495-498, Nov.-Dec. 2018. tab.
Idioma: en.
Resumo: ABSTRACT There are limited data on the molecular epidemiology of cryptococcosis in Brazil. Here, we report on the identification of the molecular pattern of the Cryptococcus species that caused meningitis in patients admitted in a Brazilian reference tertiary care hospital, and review the published studies addressing the molecular epidemiology of Cryptococcus in Brazil. Our study has shown the predominance of molecular type VNII in HIV-infected patients with cryptococcal meningoencephalitis. Molecular types VNII and VGII were occasionally detected in HIV-infected and non-infected patients with meningoencephalitis. In contrast, previous studies have shown that several regions exhibited a high prevalence of the VNI molecular type and sporadic cases of the VNII and VGII molecular types in patients with cryptococcosis in Brazil. Additional studies including VNII isolates will contribute to understanding the epidemiology and phylogenetic relationship of these genotype compared to the other ones. So far, no clear correlation has been established between genotypes, antifungal susceptibility for Cryptococcus and clinical outcome in cryptococcosis.
Descritores: Meningite Criptocócica/microbiologia
Cryptococcus neoformans/genética
-Polimorfismo de Fragmento de Restrição
Brasil/epidemiologia
Infecções por HIV/microbiologia
Técnicas de Tipagem Micológica
Meningite Criptocócica/epidemiologia
Epidemiologia Molecular
Cryptococcus neoformans/classificação
Centros de Atenção Terciária
Genótipo
Limites: Humanos
Masculino
Feminino
Lactente
Adulto
Pessoa de Meia-Idade
Idoso
Responsável: BR1.1 - BIREME


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Id: biblio-1089313
Autor: Zhao, Ting; Ye, Zhixu; Wang, Binlin; Cui, Yuxia; Nie, Yingjie; Yang, Bin; Chen, Kun; Zhang, Hua; Hu, Fangfang; Yu, Fuxun.
Título: Virus isolation and genotype identification of human respiratory syncytial virus in Guizhou Province, China
Fonte: Braz. j. infect. dis;23(6):427-434, Nov.-Dec. 2019. graf.
Idioma: en.
Projeto: Fund of the Science and Technology Department of Guizhou Province; . National Natural Science Foundation of China.
Resumo: ABSTRACT To investigate the genetic variation and molecular epidemiology characteristics of Human Respiratory Syncytial Virus (HRSV) in Guizhou Province, nasopharyngeal aspirates were collected from patients with acute respiratory infection (ARI) in Guizhou Provincial People's Hospital, from December 2017 to March 2018, and inoculated to Hep-2 cells to isolate HRSV. Cells that showed cytopathic effect (CPE) were then confirmed by indirect immunofluorescence assay and reverse transcription. The sequence of the PCR products was determined for HRSV isolates, and the genetic variation was analyzed. Out of 196 nasopharyngeal aspirate samples, HRSV were isolated in 39. The second hypervariable region at the 3' terminal of glycoprotein gene (HVR2) sequence analysis showed that subgroup A was dominant. Seventy-nine percent of the isolates belonged to subgroup A, ON1 genotype, and 21 % belonged to subgroup B, BA9 genotype, which indicates that the dominant HRSV circulating in Guizhou Province was subgroup A, genotype ON1, co-circulating with a less prevalent subgroup B, genotype BA9.
Descritores: Infecções Respiratórias/virologia
Vírus Sincicial Respiratório Humano/isolamento & purificação
Vírus Sincicial Respiratório Humano/genética
Infecções por Vírus Respiratório Sincicial/virologia
-Filogenia
Infecções Respiratórias/epidemiologia
China/epidemiologia
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Infecções por Vírus Respiratório Sincicial/epidemiologia
Epidemiologia Molecular
Genótipo
Cavidade Nasal/virologia
Limites: Humanos
Pré-Escolar
Responsável: BR1.1 - BIREME


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Id: lil-761016
Autor: Herrera Avila, Yoslany M; Fonseca Gómez, Carlos M; Gozá Valdés, Roxana; Martínez Rodríguez, Ileana M; Lemus Molina, Dihadenys; Llanes Cordero, María Josefa; Marrero Figueroa, Antonio; Díaz Rodríguez, Raúl.
Título: Tipificación con oligonucleótidos espaciadores de Mycobacterium tuberculosis en Cuba / Spacer oligonucleotide typing of Mycobacterium tuberculosis in Cuba
Fonte: Rev. cuba. med. trop;67(1):85-96, ene.-abr. 2015. tab.
Idioma: es.
Resumo: Introducción: el conocimiento de los linajes de Mycobacterium tuberculosis es importante para entender el origen, evolución y propagación de la bacteria. Objetivo: determinar los patrones genéticos de M. tuberculosis circulantes en Cuba. Métodos: estudio descriptivo de corte transversal con un componente analítico en Cuba, en el período comprendido de enero de 2009 a diciembre de 2010. Se aplicó la tipificación con oligonucleótidos espaciadores (Spoligotyping) a 308 aislamientos de M. tuberculosis del período 2009-2010. La clasificación en genotipos se realizó según la base de datos internacional SpolDB4. Los resultados se analizaron además con la herramienta web en línea MIRU-VNTRplus y se compararon con los patrones genéticos de M. tuberculosis identificados en 1993-1995 en Cuba. Resultados: se definieron 79 patrones genotípicos diferentes, de los cuales 46 (62 por ciento) fueron patrones no reportados anteriormente en SpolDB4. Los 22 agrupamientos definidos incluyeron al 75,4 por ciento de los aislamientos estudiados. Se encontraron cinco familias genéticas fundamentales: Beijing (25,6 por ciento), S (19,2 por ciento), LAM (16,9 por ciento), Haarlem (16,9 por ciento) y T (5,8 por ciento). La familia S prevaleció en la región Occidental (OR=3,4; 95 por ciento IC:1,8-6,3; p<0,05), Beijing en el Centro de Cuba (OR=6,7; 95 por ciento IC:3,7-11,9; p<0,05) y LAM (OR=3,0; 95 por ciento IC:1,6-5,6; p<0.05) y Haarlem en la zona Oriental (OR=1,8; 95 por ciento IC:1,0-3,4; p<0,05). Conclusiones: se observó una gran diversidad genética entre los aislamientos de M. tuberculosis circulante en Cuba en 2009-2010. En el país, la estructura genética de la población de M. tuberculosis ha variado en el tiempo con una disminución de genotipos endémicos como Haarlem y T y un aumento significativo de S y Beijing. Estos datos aportan elementos importantes para la epidemiología y control de la TB en Cuba(AU)

Introduction: knowledge about Mycobacterium tuberculosis lineages is important to understand the origin, evolution and spread of this bacterium. Objective: determine the genetic patterns of M. tuberculosis circulating in Cuba. Methods: adescriptive cross-sectional study was conducted with an analytical component in Cuba in the period extending from January 2009 to December 2010. Spacer oligonucleotide typing (Spoligotyping) was applied to 308 M. tuberculosis isolates from the period 2009-2010. Classification into genotypes was carried out according to the international database SpolDB4. Results were additionally analyzed with the online tool MIRU-VNTRplus and compared with the M. tuberculosis genetic patterns found in Cuba in 1993-1995. Results: 79 different genotypic patterns were defined, of which 46 (62 percent) had not been previously reported in SpolDB4. The 22 clusters defined included 75.4 percent of the isolates studied. Five main genetic families were found: Beijing (25.6 percent), S (19.2 percent), LAM (16.9 percent), Haarlem (16.9 percent) and T (5.8 percent). The S family prevailed in the Western region (OR=3.4; CI 95 percent:1.8-6.3; p<0.05), Beijing in Central Cuba (OR=6.7; CI 95 percent:3.7-11.9; p<0.05), and LAM (OR=3.0; CI 95 percent:1.6-5.6; p<0.05) and Haarlem in the Eastern region (OR=1.8; CI 95 percent:1.0-3.4; p<0.05). Conclusions: great diversity was observed among the M. tuberculosis isolates circulating in Cuba in the period 2009-2010. The genetic structure of M. tuberculosis has changed in the country with the passing of time, with a reduction in endemic genotypes like Haarlem and T, and a significant increase in S and Beijing. These data contribute important information for epidemiology and TB control in Cuba(AU)
Descritores: Oligonucleotídeos/genética
Mycobacterium tuberculosis/genética
-Epidemiologia Descritiva
Estudos Transversais
Epidemiologia Molecular/métodos
Cuba
Limites: Humanos
Masculino
Feminino
Responsável: CU1.1 - Biblioteca Médica Nacional



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