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Id: biblio-1147604
Autor: Pinto Paracampo, Nádia Elígia Nunes; Padilha de Oliveira, Maria do Socorro; Tolmie Prance, Ghillean; Poppi, Ronei Jesus.
Título: Genetic fingerprinting of the Brazilian medicinal plant Chrysobalanus icaco L. (Chrysobalanaceae)
Fonte: Brazilian Journal of Development;6(11):86190-86202, 2020. tab, ilus.
Idioma: en.
Resumo: Chrysobalanus icaco L. (Chrysobalanaceae) is a medicinal species widely used in Brazil mainly to treat diabetes. Despite the medicinal importance of C. icaco, genetic information of this genus remains limited. Thus, our aim was to evaluate the influence of the genetic basis of C. icaco by determining its chemotypes. 25 C. icaco genotypes were collected from 15 sites in Belém, Marajó and Northeastern mesoregions of Pará state, Brazil. The genotypes were selected by evaluating the plant morphological characteristics such as fruit color and plant habit. The DNA fingerprinting profile was performed using PCR based RAPD technique and appropriate statistical methods were used. RAPD markers were used for evaluation of genetic diversity and molecular characterization of the C. icaco, using a total of 18 decamer primers. These primers produced 85 amplification products, with an average of 4.7 bands per primer and 99.2% polymorphism. The genotypes are genetically distinct, forming variable clusters in number and constitution by different methods. By the morphological characteristics considered, there is a tendency of clustering based on the color of the ripe fruit. We found the secondary metabolite content depends not on environmental condition, but rather on C. icaco genome. Therefore, it may have implications for ethnopharmacological use of the chemotypes.
Descritores: Chrysobalanaceae
-Plantas Medicinais
Brasil
Impressões Digitais de DNA
Etnofarmacologia
Diabetes Mellitus
Limites: Humanos
Tipo de Publ: Relatório Técnico
Responsável: BR1.1 - BIREME


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Id: biblio-1100919
Autor: García, Patricia; Bizama, Carolina; Rosa, Lorena; Espinoza, Jaime A; Weber, Helga; Cerda-Infante, Javier; Sánchez, Marianela; Montecinos, Viviana P; Lorenzo-Bermejo, Justo; Boekstegers, Felix; Dávila-López, Marcela; Alfaro, Francisca; Leiva-Acevedo, Claudia; Parra, Zasha; Romero, Diego; Kato, Sumie; Leal, Pamela; Lagos, Marcela; Roa, Juan Carlos.
Título: Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor
Fonte: Biol. Res;53:13, 2020. tab, graf.
Idioma: en.
Projeto: Fondo Nacional de Desarrollo Científico y Tecnológico, FONDECYT; . Dirección de Investigación Medicina UC-Pontificia Universidad Católica de Chile; . Instituto Milenio IMII; . CONICYT.
Resumo: BACKGROUND: Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We describe here the establishment of three novel cell lines derived from the ascitic fluid of a Chilean GBC patient, who presented 46% European, 36% Mapuche, 12% Aymara and 6% African ancestry. RESULTS: After immunocytochemical staining of the primary cell culture, we isolated and comprehensively characterized three independent clones (PUC-GBC1, PUC-GBC2 and PUC-GBC3) by short tandem repeat DNA profiling and RNA sequencing as well as karyotype, doubling time, chemosensitivity, in vitro migration capability and in vivo tumorigenicity assay. Primary culture cells showed high expression of CK7, CK19, CA 19-9, MUC1 and MUC16, and negative expression of mesothelial markers. The three isolated clones displayed an epithelial phenotype and an abnormal structure and number of chromosomes. RNA sequencing confirmed the increased expression of cytokeratin and mucin genes, and also of TP53 and ERBB2 with some differences among the three cells lines, and revealed a novel exonic mutation in NF1. The PUC-GBC3 clone was the most aggressive according to histopathological features and the tumorigenic capacity in NSG mice. CONCLUSIONS: The first cell lines established from a Chilean GBC patient represent a new model for studying GBC in patients of Native American descent.
Descritores: Antígenos Glicosídicos Associados a Tumores/genética
Índios Sul-Americanos/genética
Neoplasias da Vesícula Biliar/genética
-Líquido Ascítico/metabolismo
Células Tumorais Cultivadas
Testes de Carcinogenicidade
Chile
Impressões Digitais de DNA
Proteína Supressora de Tumor p53/genética
Cisplatino/farmacologia
Camundongos Endogâmicos NOD
Células Clonais/efeitos dos fármacos
Células Clonais/metabolismo
Análise de Sequência de RNA
Receptor ErbB-2/genética
Genes erbB-2/genética
Perfilação da Expressão Gênica
Linhagem Celular Tumoral/efeitos dos fármacos
Linhagem Celular Tumoral/metabolismo
Desoxicitidina/análogos & derivados
Desoxicitidina/farmacologia
Células Epiteliais/metabolismo
Queratina-19/genética
Queratina-7/genética
Carcinogênese/genética
Neoplasias da Vesícula Biliar/metabolismo
Antineoplásicos/farmacologia
Limites: Humanos
Animais
Masculino
Pessoa de Meia-Idade
Responsável: CL1.1 - Biblioteca Central


  3 / 89 LILACS  
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Id: lil-588898
Autor: Cabral, Luciana Sanches; Festa Neto, Cyro; Sanches Júnior, José A; Ruiz, Itamar R. G.
Título: Genomic instability in human actinic keratosis and squamous cell carcinoma
Fonte: Clinics;66(4):523-528, 2011. ilus, tab.
Idioma: en.
Resumo: OBJECTIVE: To compare the repetitive DNA patterns of human actinic keratoses and squamous cell carcinomas to determine the genetic alterations that are associated with malignant transformation. INTRODUCTION: Cancer cells are prone to genomic instability, which is often due to DNA polymerase slippage during the replication of repetitive DNA and to mutations in the DNA repair genes. The progression of benign actinic keratoses to malignant squamous cell carcinomas has been proposed by several authors. MATERIAL AND METHODS: Eight actinic keratoses and 24 squamous cell carcinomas (SCC), which were pair-matched to adjacent skin tissues and/or leucocytes, were studied. The presence of microsatellite instability (MSI) and the loss of heterozygosity (LOH) in chromosomes 6 and 9 were investigated using nine PCR primer pairs. Random Amplified Polymorphic DNA patterns were also evaluated using eight primers. RESULTS: MSI was detected in two (D6S251, D9S50) of the eight actinic keratosis patients. Among the 8 patients who had squamous cell carcinoma-I and provided informative results, a single patient exhibited two LOH (D6S251, D9S287) and two instances of MSI (D9S180, D9S280). Two LOH and one example of MSI (D6S251) were detected in three out of the 10 patients with squamous cell carcinoma-II. Among the four patients with squamous cell carcinoma-III, one patient displayed three MSIs (D6S251, D6S252, and D9S180) and another patient exhibited an MSI (D9S280). The altered random amplified polymorphic DNA ranged from 70 percent actinic keratoses, 76 percent squamous cell carcinoma-I, and 90 percent squamous cell carcinoma-II, to 100 percent squamous cell carcinoma-III. DISCUSSION: The increased levels of alterations in the microsatellites, particularly in D6S251, and the random amplified polymorphic DNA fingerprints were statistically significant in squamous cell carcinomas, compared with actinic keratoses. CONCLUSION: The overall alterations that were observed in the repetitive DNA of actinic keratoses and squamous cell carcinomas indicate the presence of a spectrum of malignant progression.
Descritores: Carcinoma de Células Escamosas/genética
Primers do DNA/genética
Ceratose Actínica/genética
Perda de Heterozigosidade/genética
Instabilidade de Microssatélites
Neoplasias Cutâneas/genética
-Transformação Celular Neoplásica/genética
Transformação Celular Neoplásica/patologia
CHROMOSOMES, HUMAN, PAIR ABDOMEN, ACUTE
Cromossomos Humanos Par 9
Impressões Digitais de DNA
Progressão da Doença
Ceratose Actínica/patologia
Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 89 LILACS  
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Id: biblio-1102564
Autor: Briem Stamm, A. D.
Título: Actuación del odontólogo legista en catástrofes: enseñanzas del protocolo de Interpol / Forensic dentist´s role in catastrophes: teaching of the Interpol protocol
Fonte: Rev. Fac. Odontol. (B.Aires);34(76):43-51, 2019. ilus, tab.
Idioma: es.
Resumo: Una catástrofe es un acontecimiento súbito y violento que genera múltiples víctimas requiriendo del abordaje de expertos en diferentes áreas del conocimiento técnico científico para lograr una identificación inequívoca. El rol del odontólogo legista como integrante de los equipos interdisciplinarios conformados para la investigación en este tipo de hechos aparece definido en el Protocolo de Interpol, guía de operaciones estandarizada, diseñada para optimizar la comunicación, planificación, organización y estrategias entre peritos de diferentes latitudes ante incidentes naturales, accidentales o intencionales. El presente trabajo tiene por objetivo analizar las directrices de la citada norma en lo atinente a la coordinación de la actuación de los equipos de odontólogos legistas para contribuir en tales contextos, apuntada fundamentalmente a auxiliar a la justicia, aportando mayor celeridad en la identificación humana y acotando la angustia e incertidumbre de los familiares de la víctimas (AU)
Descritores: Antropologia Forense/métodos
Identificação de Vítimas
Protocolos
Desastres
Odontologia Legal
-Registros Odontológicos
Impressões Digitais de DNA
Identificação da Prótese Dentária
Limites: Humanos
Masculino
Feminino
Responsável: AR29.1 - Biblioteca


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Baptista, Ida Maria Foschiani Dias
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Id: lil-326324
Autor: Baptista, Ida Maria Foschiani Dias; Oelemann, Maraníbia Cardoso; Opromolla, Diltor Vladimir Araújo; Suffys, Philip Noel.
Título: Drug resistance and genotypes of strains of Mycobacterium tuberculosis isolated from human immunodeficiency virus-infected and non-infected tuberculosis patients in Bauru, São Paulo, Brazil
Fonte: Mem. Inst. Oswaldo Cruz;97(8):1147-1152, Dec. 15, 2002. ilus, tab.
Idioma: en.
Projeto: CNPq; . Papes; . Pronex.
Resumo: Little is known about transmission and drug resistance of tuberculosis (TB) in Bauru, State of São Paulo. The objective of this study was to evaluate risk factors for transmission of Mycobacterium tuberculosis strains in this area. Strains were collected from patients attended at ambulatory services in the region and susceptibility towards the main first line antibiotics was determined and fingerprinting performed. A total of 57 strains were submitted to susceptibility testing: 23 (42.6 percent) were resistant to at least one drug while 3 (13 percent) were resistant against both rifampicin and isoniazide. Resistant strains had been isolated from patients that had not (n = 13) or had (n = 9) previously been submitted to anti-TB treatment, demonstrating a preoccupying high level of primary resistance in the context of the study. All strains were submitted to IS6110 restriction fragment length polymorphism (IS6110-RFLP) and double repetitive element PCR (DRE-PCR). Using IS6110-RFLP, 26.3 percent of the strains were clustered and one cluster of 3 patients included 2 HIV-infected individuals that had been hospitalized together during 16 days; clustering of strains of patients from the hospital was however not higher than that of patients attended at health posts. According to DRE-PCR, 55.3 percent belonged to a cluster, confirming the larger discriminatory power of IS6110-RFLP when compared to DRE-PCR, that should therefore be used as a screening procedure only. No clinical, epidemiological or microbiological characteristics were associated with clustering so risk factors for transmission of TB could not be defined in the present study
Descritores: Rifampina
Tuberculose Pulmonar
Infecções Oportunistas Relacionadas com a AIDS
Isoniazida
Mycobacterium tuberculosis
Antituberculosos
-Polimorfismo de Fragmento de Restrição
Brasil
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase
Infecção Hospitalar
Fatores de Risco
Impressões Digitais de DNA
Genótipo
Mycobacterium tuberculosis
Limites: Humanos
Masculino
Feminino
Adulto
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  6 / 89 LILACS  
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Id: biblio-1021453
Autor: Chen, Caijin; He, Wenchuang; Yacouba Nassirou, Tondi; Nsabiyumva, Athanase; Dong, Xilong; Nevame Adedze, Yawo Mawunyo; Jin, Deming.
Título: Molecular characterization and genetic diversity of different genotypes of Oryza sativa and Oryza glaberrima
Fonte: Electron. j. biotechnol;30:48-57, nov. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Fundamental Research Funds for the Central Universities; . Specialized Research Fund for the Doctoral Program of Higher Education.
Resumo: Background: Availability of related rice species is critical for rice breeding and improvement. Two distinct species of domesticated rice exist in the genus Oryza: Oryza sativa (Asian rice) and Oryza glaberrima (African rice). New rice for Africa (NERICA) is derived from interspecific crosses between these two species. Molecular profiling of these germplasms is important for both genetics and breeding studies. We used 30 polymorphic SSR markers to assess the genetic diversity and molecular fingerprints of 53 rice genotypes of O. sativa, O. glaberrima, and NERICA. Results: In total, 180 alleles were detected. Average polymorphism information content and Shannon's information index were 0.638 and 1.390, respectively. Population structure and neighbor-joining phylogenetic tree revealed that 53 genotypes grouped into three distinct subpopulations conforming to the original three groups, except three varieties (IR66417, WAB450-4, MZCD74), and that NERICA showed a smaller genetic distance from O. sativa genotypes (0.774) than from O. glaberrima genotypes (0.889). A molecular fingerprint map of the 53 accessions was constructed with a novel encoding method based on the SSR polymorphic alleles. Ten specific SSR markers displayed different allelic profiles between the O. glaberrima and O. sativa genotypes. Conclusions: Genetic diversity studies revealed that 50 rice types were clustered into different subpopulations whereas three genotypes were admixtures. Molecular fingerprinting and 10 specific markers were obtained to identify the 53 rice genotypes. These results can facilitate the potential utilization of sibling species in rice breeding and molecular classification of O. sativa and O. glaberrima germplasms.
Descritores: Oryza/genética
Variação Genética
-Polimorfismo Genético
Cruzamento
Impressões Digitais de DNA
Repetições de Microssatélites
Genótipo
Responsável: CL1.1 - Biblioteca Central


  7 / 89 LILACS  
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Id: biblio-894908
Autor: Boechat, Jéssica Sepulveda; Oliveira, Manoel Marques Evangelista; Almeida-Paes, Rodrigo; Gremião, Isabella Dib Ferreira; Machado, Ana Caroline de Sá; Oliveira, Raquel de Vasconcelos Carvalhaes; Figueiredo, Anna Barreto Fernandes; Rabello, Vanessa Brito de Souza; Silva, Karoline Benevides de Lima; Zancopé-Oliveira, Rosely Maria; Schubach, Tânia Maria Pacheco; Pereira, Sandro Antonio.
Título: Feline sporotrichosis: associations between clinical-epidemiological profiles and phenotypic-genotypic characteristics of the etiological agents in the Rio de Janeiro epizootic area
Fonte: Mem. Inst. Oswaldo Cruz;113(3):185-196, Mar. 2018. tab, graf.
Idioma: en.
Projeto: FAPERJ; . CNPq.
Resumo: BACKGROUND Sporotrichosis is caused by species of the genus Sporothrix. From 1998 to 2015, 4,703 cats were diagnosed at the Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil. Even after the description of the Sporothrix species, the characterisation of feline isolates is not performed routinely. OBJECTIVES To characterise the clinical isolates from cats at the species level and correlate them with the clinical and epidemiological characteristics of the cats. METHODS Forty seven Sporothrix spp. isolates from cats assisted at Fiocruz from 2010 to 2011 were included. Medical records were consulted to obtain the clinical and epidemiological data. The isolates were identified through their morphological and physiological characteristics. T3B polymerase chain reaction (PCR) fingerprinting was used for molecular identification of the species. FINDINGS In phenotypic tests, 34 isolates were characterised as S. brasiliensis, one as S. schenckii and 12 as Sporothrix spp. PCR identified all isolates as S. brasiliensis. MAIN CONCLUSIONS S. brasiliensis is the only etiological agent of feline sporotrichosis in Rio de Janeiro to date. None association was found between the isolates and the clinical and epidemiological data. In addition, we strongly recommend the use of molecular techniques for the identification of isolates of Sporothrix spp.
Descritores: Sporothrix/classificação
Doenças do Gato/microbiologia
Doenças do Gato/epidemiologia
-Sporothrix/genética
Brasil/epidemiologia
Impressões Digitais de DNA
Responsável: BR1.1 - BIREME


  8 / 89 LILACS  
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Ribeiro, Márcio Garcia
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Id: biblio-842765
Autor: Bolaños, Carmen Alicia Daza; Paula, Carolina Lechinski de; Guerra, Simony Trevizan; Franco, Marília Masello Junqueira; Ribeiro, Márcio Garcia.
Título: Diagnosis of mycobacteria in bovine milk: an overview
Fonte: Rev. Inst. Med. Trop. Säo Paulo;59:e40, 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Tuberculosis remains as the world’s biggest threat. In 2014, human tuberculosis ranked as a major infectious disease by the first time, overcoming HIV death rates. Bovine tuberculosis is a chronic disease of global distribution that affects animals and can be transmitted to humans by the consumption of raw milk, representing a serious public health concern. Despite the efforts of different countries to control and eradicate bovine tuberculosis, the high negative economic impact on meat and milk production chains remains, given the decreased production efficiency (approximately 25%), the high number of condemned carcasses, and increased animal culling rates. This scenario has motivated the establishment of official programs based on regulations and diagnostic procedures. Although Mycobacterium tuberculosis and Mycobacterium bovis are the major pathogenic species to humans and bovines, respectively, nontuberculous mycobacteria within the Mycobacterium genus have become increasingly important in recent decades due to human infections, including the ones that occur in immunocompetent people. Diagnosis of mycobacteria can be performed by microbiological culture from tissue samples (lymph nodes, lungs) and secretions (sputum, milk). In general, these pathogens demand special nutrient requirements for isolation/growth, and the use of selective and rich culture media. Indeed, within these genera, mycobacteria are classified as either fast- or slow-growth microorganisms. Regarding the latter ones, incubation times can vary from 45 to 90 days. Although microbiological culture is still considered the gold standard method for diagnosis, molecular approaches have been increasingly used. We describe here an overview of the diagnosis of Mycobacterium species in bovine milk.
Descritores: Bovinos/microbiologia
Leite/microbiologia
Mycobacterium/isolamento & purificação
-Impressões Digitais de DNA/métodos
Mycobacterium/genética
Reação em Cadeia da Polimerase/métodos
Tuberculose Bovina/epidemiologia
Tuberculose Bovina/microbiologia
Limites: Humanos
Animais
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  9 / 89 LILACS  
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Id: lil-796905
Autor: Vélez, Norida; Escandón, Patricia.
Título: Distribution and association between environmental and clinical isolates of Cryptococcus neoformans in Bogotá-Colombia, 2012-2015
Fonte: Mem. Inst. Oswaldo Cruz;111(10):642-648, Oct. 2016. tab, graf.
Idioma: en.
Projeto: Instituto Nacional de Salud.
Resumo: The propagules of the fungal species Cryptococcus neoformans and C. gattii, whose varieties are distributed world wide, are the primary cause of cryptococcosis, a life threatening disease. The study of environmental and clinical isolates of Cryptococcosis is an important contribution to the epidemiology and ecology of the fungus. The aim of this work was to determine the presence of C. neoformans and C. gattii in the environment in Bogotá, Colombia’s capital city and to establish the relation between clinical and environmental isolates in the period 2012-2015. From a total of 4.116 environmental samples collected between October 2012 - March 2014, 35 were positive for C. neoformans var. grubii. From 55 cryptococcosis cases reported in Bogotá during 2012-2015, 49 isolates were recovered. From those, 94% were identified as C. neoformans var. grubii molecular type VNI; 4% as VNII and 1,2% as C. neoformans var neoformans VNIV. The 84 detected clinical and environmental isolates studied had a similarity between 49-100% according with molecular typing. The correlation between environmental and clinical samples confirms the hypothesis that patients acquire the disease from environmental exposure to the fungal propagules.
Descritores: Criptococose/microbiologia
Cryptococcus gattii/isolamento & purificação
Cryptococcus neoformans/isolamento & purificação
Microbiologia Ambiental
-Cidades
Colômbia
Cryptococcus gattii/genética
Cryptococcus neoformans/genética
Impressões Digitais de DNA
DNA Fúngico
Genótipo
Tipagem Molecular
Técnicas de Tipagem Micológica
Limites: Humanos
Masculino
Feminino
Responsável: BR1.1 - BIREME


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Id: lil-775120
Autor: Šuranská, Hana; Vránová, Dana; Omelková, Jiřina.
Título: Isolation, identification and characterization of regional indigenous Saccharomyces cerevisiae strains
Fonte: Braz. j. microbiol;47(1):181-190, Jan.-Mar. 2016. tab, graf.
Idioma: en.
Projeto: MŠMT ČR.
Resumo: Abstract In the present work we isolated and identified various indigenous Saccharomyces cerevisiae strains and screened them for the selected oenological properties. These S. cerevisiae strains were isolated from berries and spontaneously fermented musts. The grape berries (Sauvignon blanc and Pinot noir) were grown under the integrated and organic mode of farming in the South Moravia (Czech Republic) wine region. Modern genotyping techniques such as PCR-fingerprinting and interdelta PCR typing were employed to differentiate among indigenous S. cerevisiae strains. This combination of the methods provides a rapid and relatively simple approach for identification of yeast of S. cerevisiae at strain level. In total, 120 isolates were identified and grouped by molecular approaches and 45 of the representative strains were tested for selected important oenological properties including ethanol, sulfur dioxide and osmotic stress tolerance, intensity of flocculation and desirable enzymatic activities. Their ability to produce and utilize acetic/malic acid was examined as well; in addition, H2S production as an undesirable property was screened. The oenological characteristics of indigenous isolates were compared to a commercially available S. cerevisiae BS6 strain, which is commonly used as the starter culture. Finally, some indigenous strains coming from organically treated grape berries were chosen for their promising oenological properties and these strains will be used as the starter culture, because application of a selected indigenous S. cerevisiae strain can enhance the regional character of the wines.
Descritores: Saccharomyces cerevisiae/classificação
Saccharomyces cerevisiae/isolamento & purificação
Vitis/microbiologia
-Ácido Acético/metabolismo
Aderência Bacteriana
República Tcheca
Impressões Digitais de DNA
Tolerância a Medicamentos
Etanol/toxicidade
Sulfeto de Hidrogênio/metabolismo
Tipagem Molecular
Técnicas de Tipagem Micológica
Malatos/metabolismo
Pressão Osmótica
Reação em Cadeia da Polimerase
Estresse Fisiológico
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/fisiologia
Dióxido de Enxofre/toxicidade
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME



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