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Pesquisa : E05.393.350 [Categoria DeCS]
Referências encontradas : 61 [refinar]
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Id: biblio-1001521
Autor: Volobueva, A S; Orekhov, A N; Deykin, A V.
Título: An update on the tools for creating transgenic animal models of human diseases - focus on atherosclerosis
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;52(5):e8108, 2019. tab.
Idioma: en.
Projeto: Russian Science Foundation.
Resumo: Animal models of diseases are invaluable tools of modern medicine. More than forty years have passed since the first successful experiments and the spectrum of available models, as well as the list of methods for creating them, have expanded dramatically. The major step forward in creating specific disease models was the development of gene editing techniques, which allowed for targeted modification of the animal's genome. In this review, we discuss the available tools for creating transgenic animal models, such as transgenesis methods, recombinases, and nucleases, including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and CRISPR/Cas9 systems. We then focus specifically on the models of atherosclerosis, especially mouse models that greatly contributed to improving our understanding of the disease pathogenesis and we outline their characteristics and limitations.
Descritores: Animais Geneticamente Modificados
Engenharia Genética/métodos
Modelos Animais de Doenças
Aterosclerose/fisiopatologia
Nucleases dos Efetores Semelhantes a Ativadores de Transcrição/metabolismo
-Técnicas de Transferência de Genes
Pesquisa Biomédica/métodos
Aterosclerose/genética
Limites: Humanos
Animais
Masculino
Feminino
Coelhos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  2 / 61 LILACS  
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Id: biblio-952830
Autor: Strauss, Bryan E; Silva, Gissele Rolemberg Oliveira; de Luna Vieira, Igor; Cerqueira, Otto Luiz Dutra; Del Valle, Paulo Roberto; Medrano, Ruan Felipe Vieira; Mendonça, Samir Andrade.
Título: Perspectives for cancer immunotherapy mediated by p19Arf plus interferon-beta gene transfer
Fonte: Clinics;73(supl.1):e479s, 2018. graf.
Idioma: en.
Projeto: IVL; . MGL; . PRDV; . RFVM; . BES.
Resumo: While cancer immunotherapy has gained much deserved attention in recent years, many areas regarding the optimization of such modalities remain unexplored, including the development of novel approaches and the strategic combination of therapies that target multiple aspects of the cancer-immunity cycle. Our own work involves the use of gene transfer technology to promote cell death and immune stimulation. Such immunogenic cell death, mediated by the combined transfer of the alternate reading frame (p14ARF in humans and p19Arf in mice) and the interferon-β cDNA in our case, was shown to promote an antitumor immune response in mouse models of melanoma and lung carcinoma. With these encouraging results, we are now setting out on the road toward translational and preclinical development of our novel immunotherapeutic approach. Here, we outline the perspectives and challenges that we face, including the use of human tumor and immune cells to verify the response seen in mouse models and the incorporation of clinically relevant models, such as patient-derived xenografts and spontaneous tumors in animals. In addition, we seek to combine our immunotherapeutic approach with other treatments, such as chemotherapy or checkpoint blockade, with the goal of reducing dosage and increasing efficacy. The success of any translational research requires the cooperation of a multidisciplinary team of professionals involved in laboratory and clinical research, a relationship that is fostered at the Cancer Institute of Sao Paulo.
Descritores: Terapia Genética/métodos
Fases de Leitura/genética
Interferon beta/uso terapêutico
Técnicas de Transferência de Genes
Imunoterapia/métodos
Neoplasias/terapia
-Morte Celular/genética
Inibidor p16 de Quinase Dependente de Ciclina/genética
Proteína Supressora de Tumor p14ARF/genética
Neoplasias/imunologia
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  3 / 61 LILACS  
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Id: biblio-997849
Autor: Manrique, Jorge Luis.
Título: Límite ético a la manipulación genética / Ethical limits to genetic manipulation
Fonte: Bol. Acad. Nac. Med. B.Aires;93(1):111-122, ene.-jun. 2015. ilus.
Idioma: es.
Conferência: Apresentado em: Encuentro Interacadémico, 3, Apresentado em: Principio y Fin de la Vida Humana, Ciudad Autónoma de Buenos Aires, 22 mayo 2015.
Descritores: Engenharia Genética/ética
Técnicas de Transferência de Genes/ética
Clonagem de Organismos/ética
-Bioética
Genoma Humano
Responsável: AR1.1 - Biblioteca Rafael Herrera Vegas


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Id: biblio-1087255
Autor: Arencibia, Ariel D; D'Afonseca, Vívian; Chakravarthi, Mohan; Castiglione, Stefano.
Título: Learning from transgenics: advanced gene editing technologies should also bridge the gap with traditional genetic selection
Fonte: Electron. j. biotechnol;41:22-29, sept. 2019. ilus.
Idioma: en.
Resumo: We highlight the importance of the mixed genetic approaches (classical and currents) to improve the social perception related to the GMOs acceptance. We pointed out that CRISPR/Cas9 events could carry DNA variability/rearrangements related to somaclonal variations or epigenetic changes that are independent from the editing per se. The transformation of single cells, followed by plant regeneration, is used to generate modified plants, transgenic or genome editing (CRISPR/Cas9). The incidence of undesirable somaclonal variations and/or epigenetic changes that might have occurred during in vitro multiplication and regeneration processes, must be carefully analyzed in replicates in field trials. One remarkable challenge is related to the time lapse that selects the modified elite genotypes, because these strategies may spend a variable amount of time before the results are commercialized, where in all the cases it should be take into account the genotype × environment interactions. Furthermore, this combination of techniques can create an encouraging bridge between the public opinion and the community of geneticists who are concerned with plant genetic improvement. In this context, either transgenesis or genomic editing strategies become complementary modern tools to facing the challenges of plant genetic improvement. Their applications will depend on case-by-case analysis, and when possible will necessary associate them to the schemes and bases of classic plant genetic improvement.
Descritores: Plantas Geneticamente Modificadas
Técnicas de Transferência de Genes
Sistemas CRISPR-Cas
Edição de Genes
-Transformação Genética
Mutagênese
Metilação de DNA
Melhoramento Genético
Epigênese Genética
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
Beçak, Willy
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Id: lil-480662
Autor: Góes, Luiz Gustavo Bentim; Freitas, Antonio Carlos de; Ferraz, Oilita Pereira; Rieger, Tania Tassinari; Santos, José Ferreira dos; Pereira, Alexandre; Beçak, Willy; Lindsey, Charles J; Stocco, Rita de Cassia.
Título: Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression
Fonte: Braz. j. microbiol;39(1):1-4, Jan.-Mar. 2008. ilus.
Idioma: en.
Resumo: The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. BPV protein L1 gene transfection in the Drosophila melanogaster S2 cell expression system failed to produce L1 protein notwithstanding correct L1 gene insertion. Severe genetic inbalance in the host cell line, including cytogenetic alterations, may account for the lack of protein expression.

O desenvolvimento de uma vacina para papilomavirus bovino (BPV) consiste em grande desafio. A transfecção do gene codificante da proteína L1 de BPV em sistema de células S2 de Drosophila melanogaster não logrou sucesso, apesar da correta inserção da seqüência gênica em vetor apropriado.Graves alterações genéticas na linhagem celular S2, que incluem aberrações cromossômicas, provavelmente estão relacionadas à ausência da expressão da proteína desejada.
Descritores: Drosophila melanogaster/genética
Técnicas In Vitro
Infecções por Papillomavirus
Papillomavirus Bovino 1/isolamento & purificação
Técnicas de Transferência de Genes
Vacinas contra Papillomavirus/genética
-Métodos
Métodos
Limites: Animais
Bovinos
Tipo de Publ: Relatório Técnico
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-623748
Autor: Boman, H. G; Faye, I; Gan, R; Gudmundsson, G. H; Lidholm, D. A; Lee, J. Y; Xanthopoulos, K. G.
Título: Insect immunity: a gene system for antibacterial proteins
Fonte: Mem. Inst. Oswaldo Cruz;82(supl.3):115-124, 1987. tab, ilus.
Idioma: en.
Conferência: Apresentado em: International Symposium on Insect Physiology, Biochemistry and Control, Rio de Janeiro, Nov. 10-13, 1987.
Descritores: Farmacorresistência Bacteriana
Antibacterianos/análise
-Técnicas de Transferência de Genes
Insetos/imunologia
Responsável: BR1.1 - BIREME


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Id: biblio-1021034
Autor: Mihálik, Daniel; Gubisová, Marcela; Kraic, Ján; Hudcovicová, Martina; Havrlentová, Michaela; Moravcíková, Jana; Glasa, Miroslav; Matusíková, Ildikó.
Título: Introduction of a synthetic Thermococcus-derived α-amlyase gene into barley genome for increased enzyme thermostability in grains
Fonte: Electron. j. biotechnol;30:1-5, nov. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Slovak Research and Development Agency.
Resumo: Background: The enzymes utilized in the process of beer production are generally sensitive to higher temperatures. About 60% of them are deactivated in drying the malt that limits the utilization of starting material in the fermentation process. Gene transfer from thermophilic bacteria is a promising tool for producing barley grains harboring thermotolerant enzymes. Results: Gene for α-amylase from hydrothermal Thermococcus, optimally active at 75­85°C and pH between 5.0 and 5.5, was adapted in silico to barley codon usage. The corresponding sequence was put under control of the endosperm-specific promoter 1Dx5 and after synthesis and cloning transferred into barley by biolistics. In addition to model cultivar Golden Promise we transformed three Slovak barley cultivars Pribina, Levan and Nitran, and transgenic plants were obtained. Expression of the ~50 kDa active recombinant enzyme in grains of cvs. Pribina and Nitran resulted in retaining up to 9.39% of enzyme activity upon heating to 75°C, which is more than 4 times higher compared to non-transgenic controls. In the model cv. Golden Promise the grain α-amylase activity upon heating was above 9% either, however, the effects of the introduced enzyme were less pronounced (only 1.22 fold difference compared with non-transgenic barley). Conclusions: Expression of the synthetic gene in barley enhanced the residual α-amylase activity in grains at high temperatures.
Descritores: Sementes/enzimologia
Hordeum/enzimologia
Thermococcus/metabolismo
alfa-Amilases/metabolismo
-Sementes/genética
Sementes/microbiologia
Transformação Genética
Hordeum/genética
Hordeum/microbiologia
Cerveja
Estabilidade Enzimática
Plantas Geneticamente Modificadas/enzimologia
Clonagem Molecular
Técnicas de Transferência de Genes
alfa-Amilases/genética
Fermentação
Termotolerância
Temperatura Alta
Concentração de Íons de Hidrogênio
Responsável: CL1.1 - Biblioteca Central


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Burin, Maira Graeff
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Id: biblio-876699
Autor: Poswar, Fabiano de Oliveira; Mayer, Fabiana Quoos; Burin, Maira Graeff; Matte, Ursula da Silveira; Giugliani, Roberto; Baldo, Guilherme.
Título: Long-term restoration of alpha-L-iduronidase activity in fibroblasts from patients with mucopolysaccharidosis type I after non-viral gene transfer
Fonte: Clin. biomed. res;37(4):330-333, 2017. ilus, graf.
Idioma: en.
Resumo: Mucopolysaccharidosis type I (MPS I) is a lysosomal storage disorder caused by deficiency of alpha-L-iduronidase (IDUA). Limitations such as the need for weekly injections, high morbidity and mortality, and high cost of current treatments show that new approaches to treat this disease are required. In this study, we aimed to correct fibroblasts from a patient with MPS I using non-viral gene therapy. Using a plasmid encoding the human IDUA cDNA, we achieved stable high IDUA levels in transfected fibroblasts up to 6 months of treatment. These results serve as proof of concept that a non-viral approach can correct the enzyme deficiency in cells of patients with lysosomal storage disorders, which can be used as a research tool for a series of disease aspects. Future studies should focus on showing if this approach can be useful in small animals and clinical trials (AU)
Descritores: Fibroblastos/enzimologia
Técnicas de Transferência de Genes
Vetores Genéticos
Iduronidase/metabolismo
Mucopolissacaridose I/terapia
-DNA Complementar
Terapia Genética/métodos
Iduronidase/genética
Mucopolissacaridose I/genética
Plasmídeos/genética
Transfecção/métodos
Limites: Humanos
Responsável: BR18.1 - Biblioteca FAMED/HCPA


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Texto completo SciELO Chile
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Id: lil-764087
Autor: Zegpi, María Soledad; Downey, Camila; Vial-Letelier, Verónica.
Título: Ectasia ductal mamaria en niños, a propósito de un caso clínico: A case report / Mammary duct ectasia in children
Fonte: Rev. chil. pediatr;86(4):287-290, ago. 2015. ilus, graf.
Idioma: es.
Resumo: Introducción: La telorragia es un síntoma poco frecuente en pacientes pediátricos, la causa más frecuente en esta población es la ectasia ductal mamaria (EDM), que es una afección benigna y autolimitada, caracterizada por la dilatación del conducto mamario, fibrosis e inflamación periductal. Objetivo: Presentar un caso de EDM, para facilitar el rápido reconocimiento por parte de los médicos, y evitar estudios y tratamientos agresivos. Caso clínico: Lactante de sexo masculino de 6 meses de edad, sano, alimentado por lactancia materna exclusiva; consultó por un nódulo retroareolar derecho y telorragia unilateral. Se realizó una ecografía Doppler que mostró una lesión multiquística, sugerente de una EDM. Se planteó tratamiento expectante y acudió a control a los 6 meses con excelente evolución. Conclusiones: La EDM es la principal causa de telorragia en niños, corresponde a una afección benigna, y la resolución generalmente es espontánea, antes de los 9 meses. Por lo que su conocimiento es de gran relevancia para el adecuado diagnóstico y manejo de estos pacientes.

Introduction: Bloody nipple discharge is an infrequent symptom during childhood. The most common cause in this population is mammary duct ectasia (MDE), which is a benign and self-limiting condition, that is characterized by dilatation of the mammary ducts, fibrosis and periductal inflammation. Objective: Report of a case of MDE in order to improve physicians’ diagnosis accuracy and avoid aggressive studies and treatments. Case report: Six-months old male healthy infant, exclusively breastfeeded, that visited our clinic with a lump beneath his right nipple and bloody discharge from the same nipple. An ultrasound was performed which showed a multicystic lesion suggestive of MDE. Watchful waiting was decided as treatment, with good evolution after six months of follow up. Conclusions: The MDE is the leading cause of bloody discharge in pediatric population, being a benign condition that resolves spontaneously before nine months. The knowledge of this condition is essential so as to accurately diagnose and treat it.
Descritores: Cátions/química
Indicadores e Reagentes/química
Lipídeos/química
Polienos/química
RNA Interferente Pequeno/química
-Linhagem Celular Tumoral
Química Farmacêutica/métodos
Técnicas de Transferência de Genes
Vetores Genéticos/genética
Células HeLa
Lipossomos/química
Luciferases/química
Fosfolipídeos/química
RNA Interferente Pequeno/genética
Transfecção/métodos
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: lil-764081
Autor: Castillo C, Carmen; Santander M, Dolores; Solís F, Fresia.
Título: Nivel de autoconcepto en niños con secuelas de quemaduras: estudio comparativo / Self-concept level in children with burns sequelae: A comparative study
Fonte: Rev. chil. pediatr;86(4):251-256, ago. 2015. tab.
Idioma: es.
Resumo: Introducción: Autoconcepto es el conjunto de ideas y actitudes que se tiene acerca de sí mismo. Nuestro objetivo fue evaluar si existen diferencias en el nivel de autoconcepto de niños de 8-12 años con y sin secuelas de quemaduras, e identificar variables predictoras del autoconcepto en los niños con secuelas. Pacientes y método: Estudio comparativo, transversal de 109 niños con secuelas de quemaduras de 8 a 12 años de edad, con 109 niños sin secuelas de quemaduras, del mismo grupo de edad y nivel socioeconómico. Se utilizó la escala de autoconcepto de Piers-Harris, que entrega medida de autoconcepto general y dimensiones: conductual, estatus intelectual y escolar, apariencia y atributos físicos, ansiedad, popularidad, felicidad y satisfacción. Resultados: No hubo diferencias significativas en el nivel de autoconcepto general ni en sus dimensiones al comparar ambos grupos (p > 0,05). Dentro del grupo con secuelas de quemaduras la variable número de secuelas resultó ser un factor protector para las dimensiones ansiedad, popularidad, felicidad-satisfacción y autoconcepto general. La variable localización surgió como factor de riesgo para la dimensión conductual. Discusión: La ausencia de diferencias en autoconcepto entre niños con secuelas de quemaduras y sin ellas es similar a lo reportado por la literatura. El hallazgo en factores de riesgo y protectores motiva a continuar investigando, incorporando antecedentes premórbidos y familiares.

Introduction: Self-concept is the set of ideas and attitudes that a person has about him/herself. Objective: To evaluate whether there are differences in the level of self-concept in children 8-12 years old with and without burns sequelae. To identify predictive variables of self-concept in children with sequelae. Patients and method: A comparative cross-sectional study of self-concept in 109 children with burns sequelae, from 8 to 12 years old, with 109 children without burns sequelae, and of the same age and socioeconomic status. The Piers-Harris self-concept scale is used, which provides a general measurement of self-concept and behavioural, intellectual and school status, appearance, and physical attributes, anxiety, popularity, happiness and satisfaction dimensions. Results: There were no significant differences in the level of general self-concept or their dimensions (P > .05). In the group with burns sequelae, the protective factor was the variable number of sequels was associated with the dimensions of anxiety, popularity, happiness-satisfaction and general self-concept. The location variable emerged as a risk factor for the behavioural dimension. Discussion: The absence of differences in self-concept between children with burns sequelae and children without them is similar to that reported in the literature. The finding in the risk and protective factors encourages to further research, and perhaps incorporating pre-morbidity and family background.
Descritores: DNA
Compostos de Amônio Quaternário/química
Serina/química
Tensoativos/química
-Amidas/química
Aminas/química
Linhagem Celular Tumoral
Química Farmacêutica/métodos
Ésteres/química
Técnicas de Transferência de Genes
Terapia Genética/métodos
Células HeLa
Lipídeos/química
Transfecção/métodos
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central



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