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Id: biblio-889176
Autor: Li, Li; Mu, Lan; Wang, Xiaojuan; Yu, Jingfeng; Hu, Ruiping; Li, Zhen.
Título: A novel expression vector for the secretion of abaecin in Bacillus subtilis
Fonte: Braz. j. microbiol;48(4):809-814, Oct.-Dec. 2017. graf.
Idioma: en.
Resumo: ABSTRACT This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter Pglv, and a mature beta-glucanase signal peptide. Also, a B. subtilis expression system was constructed. The recombinant abaecin gene was expressed and purified as a recombinant protein in the culture supernatant. The purified abaecin did not inhibit the growth of Escherichia coli strain K88. Cecropin A and hymenoptaecin exhibited potent bactericidal activities at concentrations of 1 and 1.5 µM. Combinatorial assays revealed that cecropin A and hymenoptaecin had sublethal concentrations of 0.3 and 0.5 µM. This potentiating functional interaction represents a promising therapeutic strategy. It provides an opportunity to address the rising threat of multidrug-resistant pathogens that are recalcitrant to conventional antibiotics.
Descritores: Peptídeos Catiônicos Antimicrobianos/genética
Peptídeos Catiônicos Antimicrobianos/metabolismo
Bacillus subtilis/genética
Vetores Genéticos/genética
Proteínas de Insetos/genética
Proteínas de Insetos/metabolismo
-Antibacterianos/isolamento & purificação
Antibacterianos/metabolismo
Antibacterianos/farmacologia
Peptídeos Catiônicos Antimicrobianos/isolamento & purificação
Peptídeos Catiônicos Antimicrobianos/farmacologia
Bacillus subtilis/metabolismo
Escherichia coli/efeitos dos fármacos
Escherichia coli/crescimento & desenvolvimento
Expressão Gênica
Vetores Genéticos/metabolismo
Proteínas de Insetos/isolamento & purificação
Proteínas de Insetos/farmacologia
Engenharia de Proteínas
Transporte Proteico
Proteínas Recombinantes/genética
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/metabolismo
Proteínas Recombinantes/farmacologia
Responsável: BR1.1 - BIREME


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Id: biblio-834064
Autor: Hounkpe, Bidossessi Wilfried; Paula, Erich Vinicius de.
Título: Bioengineering coagulation factors for improved hemophilia treatments Comment on: the mutation F309S increases FVIII secretion in human cell line
Fonte: Rev. bras. hematol. hemoter;38(3):184-185, 2016.
Idioma: en.
Descritores: Bioengenharia
Fator IX
Fator VIII
Hemofilia A/terapia
Mutagênese
-Coagulação Sanguínea
Engenharia de Proteínas
Limites: Humanos
Tipo de Publ: Comentário
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: lil-742298
Autor: Pires, Carla; Vigário, Marina; Cavaco, Afonso.
Título: Readability of medicinal package leaflets: a systematic review / Legibilidade das bulas dos medicamentos: revisão sistemática
Fonte: Rev. saúde pública = J. public health;49:1-13, 27/02/2015. tab, graf.
Idioma: en.
Projeto: Fundação para a Ciência e Tecnologia, Ministério da Educação e Ciência, Portugal.
Resumo: OBJECTIVE To review studies on the readability of package leaflets of medicinal products for human use. METHODS We conducted a systematic literature review between 2008 and 2013 using the keywords “Readability and Package Leaflet” and “Readability and Package Insert” in the academic search engine Biblioteca do Conhecimento Online, comprising different bibliographic resources/databases. The preferred reporting items for systematic reviews and meta-analyses criteria were applied to prepare the draft of the report. Quantitative and qualitative original studies were included. Opinion or review studies not written in English, Portuguese, Italian, French, or Spanish were excluded. RESULTS We identified 202 studies, of which 180 were excluded and 22 were enrolled [two enrolling healthcare professionals, 10 enrolling other type of participants (including patients), three focused on adverse reactions, and 7 descriptive studies]. The package leaflets presented various readability problems, such as complex and difficult to understand texts, small font size, or few illustrations. The main methods to assess the readability of the package leaflet were usability tests or legibility formulae. Limitations with these methods included reduced number of participants; lack of readability formulas specifically validated for specific languages (e.g., Portuguese); and absence of an assessment on patients literacy, health knowledge, cognitive skills, levels of satisfaction, and opinions. CONCLUSIONS Overall, the package leaflets presented various readability problems. In this review, some methodological limitations were identified, including the participation of a limited number of patients and healthcare professionals, the absence of prior assessments of participant literacy, humor or sense of satisfaction, or the predominance of studies not based on role-plays about the use of medicines. These limitations should be avoided in future ...

OBJECTIVO Analisar a literatura sobre legibilidade das bulas dos medicamentos para uso humano. MÉTODOS Estudo de revisão sistemática, utilizando as palavras-chave “Readability and Package Leaflet” e “Readability and Package Insert”e a ferramenta de busca académica b-on, que contém diferentes bases bibliográficas. O período analisado foi entre 2008 e 2013. Foram aplicados os critérios PRISMA para redigir o relatório da revisão. Foram incluídos artigos originais de pesquisa quantitativa ou qualitativa. Os critérios de exclusão foram: artigos de opinião ou de revisão, ou escritos numa língua diferente do inglês, português, italiano, francês ou espanhol. RESULTADOS Foram identificados 202 trabalhos, dos quais 180 foram excluídos e 22 selecionados para análise: dois com profissionais de saúde, 10 com pacientes, três sobre reações adversas e sete descritivos. As bulas apresentaram diversos problemas de legibilidade, entre os quais: textos insuficientemente claros e simples, utilização de tamanhos de letra pequenos e número reduzido de ilustrações. Os principais métodos utilizados para avaliar a legibilidade das bulas foram as fórmulas e os testes de legibilidade/usabilidade. Entre as limitações metodológicas, foram identificados aspetos como o recurso a amostras pequenas, a inexistência de fórmulas de legibilidade específicas para a língua em causa, e.g., português, e a realização de testes de compreensão em grupos de pacientes sem avaliação prévia da literacia, dos conhecimentos específicos na área da saúde, das capacidades cognitivas, ou do grau de satisfação dos participantes. CONCLUSÕES Em geral, as bulas apresentaram diversos problemas de legibilidade. Adicionalmente, nesta revisão foram identificadas algumas limitações metodológicas nos estudos revistos (e.g. a participação de um número reduzido de pacientes e profissionais de saúde, a ausência da avaliação prévia da literacia, do humor ou satisfação dos participantes ...
Descritores: Fatores de Coagulação Sanguínea/farmacologia
Fatores de Coagulação Sanguínea/uso terapêutico
Hemostasia/efeitos dos fármacos
-Anticorpos Monoclonais
Antitrombinas
Proteínas Antitrombina/genética
Biotecnologia
Ensaios Clínicos como Assunto
Fator IX
Fator VIIa
Fator VIII
Hemostasia/fisiologia
Engenharia de Proteínas
Interferência de RNA
Proteínas Recombinantes/farmacologia
Proteínas Recombinantes/uso terapêutico
Falha de Tratamento
Resultado do Tratamento
Limites: Animais
Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: lil-712968
Autor: Sun, H.; Wu, G.M.; Chen, Y.Y.; Tian, Y.; Yue, Y.H.; Zhang, G.L..
Título: Expression, production, and renaturation of a functional single-chain variable antibody fragment (scFv) against human ICAM-1
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(7):540-547, 07/2014. tab, graf.
Idioma: en.
Projeto: the Academy of Military Medical Sciences of the People's Liberation Army.
Resumo: Intercellular adhesion molecule-1 (ICAM-1) is an important factor in the progression of inflammatory responses in vivo. To develop a new anti-inflammatory drug to block the biological activity of ICAM-1, we produced a monoclonal antibody (Ka=4.19×10−8 M) against human ICAM-1. The anti-ICAM-1 single-chain variable antibody fragment (scFv) was expressed at a high level as inclusion bodies in Escherichia coli. We refolded the scFv (Ka=2.35×10−7 M) by ion-exchange chromatography, dialysis, and dilution. The results showed that column chromatography refolding by high-performance Q Sepharose had remarkable advantages over conventional dilution and dialysis methods. Furthermore, the anti-ICAM-1 scFv yield of about 60 mg/L was higher with this method. The purity of the final product was greater than 90%, as shown by denaturing gel electrophoresis. Enzyme-linked immunosorbent assay, cell culture, and animal experiments were used to assess the immunological properties and biological activities of the renatured scFv.
Descritores: Expressão Gênica/fisiologia
Fragmentos de Imunoglobulinas/biossíntese
Molécula 1 de Adesão Intercelular/imunologia
Redobramento de Proteína
Renaturação Proteica
Anticorpos de Cadeia Única/biossíntese
-Complexo Antígeno-Anticorpo
Anti-Inflamatórios/farmacologia
Anticorpos Monoclonais/biossíntese
Adesão Celular
Cromatografia
Diálise
Ensaio de Imunoadsorção Enzimática
Pavilhão Auricular/efeitos dos fármacos
Escherichia coli/genética
Vetores Genéticos
Fragmentos de Imunoglobulinas/farmacologia
Corpos de Inclusão/metabolismo
Molécula 1 de Adesão Intercelular/efeitos dos fármacos
Leucócitos Mononucleares/metabolismo
Plasmídeos
Engenharia de Proteínas/métodos
Anticorpos de Cadeia Única/farmacologia
Xilenos/farmacologia
Limites: Animais
Feminino
Humanos
Masculino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-700402
Autor: Martínez, Ronny; Schwaneberg, Ulrich.
Título: A roadmap to directed enzyme evolution and screening systems for biotechnological applications
Fonte: Biol. Res;46(4):395-405, 2013. ilus, tab.
Idioma: en.
Resumo: Enzymes have been long used in man-made biochemical processes, from brewing and fermentation to current industrial production of fine chemicals. The ever-growing demand for enzymes in increasingly specific applications requires tailoring naturally occurring enzymes to the non-natural conditions found in industrial processes. Relationships between enzyme sequence, structure and activity are far from understood, thus hindering the capacity to design tailored biocatalysts. In the field of protein engineering, directed enzyme evolution is a powerful algorithm to generate and identify novel and improved enzymes through iterative rounds of mutagenesis and screening applying a specific evolutive pressure. In practice, critical checkpoints in directed evolution are: selection of the starting point, generation of the mutant library, development of the screening assay and analysis of the output of the screening campaign. Each step in directed evolution can be performed using conceptually and technically different approaches, all having inherent advantages and challenges. In this article, we present and discuss in a general overview, challenges of designing and performing a directed enzyme evolution campaign, current advances in methods, as well as highlighting some examples of its applications in industrially relevant enzymes.
Descritores: Biotecnologia/métodos
Evolução Molecular Direcionada/métodos
Enzimas/metabolismo
Engenharia de Proteínas/métodos
-Biocatálise
Enzimas/química
Enzimas/genética
Mutagênese
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


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Id: lil-614600
Autor: Vaz, Michelle Rossana Ferreira; França, Ricardo Luiz Soares de; Andrade, Sirtys Santos Lessa de; Sousa Junior, Francisco Canindé de; Santos, Everaldo Silvino dos; Martins, Daniella Regina Arantes; Macedo, Gorete Ribeiro de.
Título: Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli
Fonte: Braz. j. microbiol;42(4):1390-1396, Oct.-Dec. 2011. ilus.
Idioma: en.
Resumo: With the advent of recombinant DNA technology, recombinant protein expression has become an important tool in the study of the structure, function and identification of new proteins, especially those with therapeutic functions. Escherichia coli has been the predominant prokaryote used in genetic engineering studies due to the abundance of information about its metabolism. Despite significant advances in molecular biology and immunology of infections, there are as yet no prophylactic drugs capable of preventing visceral leishmaniasis. It is therefore important to identify specific antigens in order to develop vaccines and diagnostic kits against this disease. The objective of this study was to evaluate the influence of culture medium on the production of eIF antigen from Leishmania chagasi in recombinant Escherichia coli. An induction procedure using IPTG was carried out in a series of trials, to observe the influence of culture medium (2xTY, TB) under expression of the recombinant eIF protein. Results showed that recombinant protein expression was associated to growth and that the highest eIF antigen expression was obtained in the 2xTY medium.
Descritores: Escherichia coli/genética
Leishmaniose Visceral
Engenharia de Proteínas
Proteínas/análise
Proteínas Recombinantes
-Microbiologia Industrial
Métodos
Técnicas
Tipo de Publ: Estudo de Avaliação
Responsável: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-601518
Autor: Escorcia, Andrés; Cruz, Jenniffer; Torres, Rodrigo; Ortiz, Claudia.
Título: Resolución cinética de (R, S)-mandelato de metilo por preparaciones inmovilizadas de lipasa de Candida antarctica B / Kinetic resolution of (R, S)-methyl mandelate by immobilized lipase preparations from Candida antarctica B
Fonte: Vitae (Medellín);18(1):33-41, ene.-abr. 2011.
Idioma: es.
Resumo: El desarrollo de métodos para la obtención de compuestos quirales constituye uno de los grandes desafíos de la química actual. En este contexto, la resolución cinética catalizada por lipasas representa una excelente alternativa. En medios homogéneos, estas enzimas presentan equilibrio entre dos conformaciones (abierta y cerrada), el cual es posible desplazar hacia la conformación abierta (forma activa) en presencia de soportes hidrofóbicos. En este trabajo, la lipasa de Candida antarctica B (CAL-B) se purificó e inmovilizó covalentemente en soportes epóxido Eupergit C (EC), Eupergit C activados con otros grupos funcionales y soportes de octil-agarosa. Estas preparaciones de enzima inmovilizada se utilizaron bajo diferentes condiciones de pH en la resolución cinética del (R,S)-mandelato de metilo. Se destacó el derivado inmovilizado EC-amino-CAL-B, por ser altamente enantioselectivo a pH 8 (razón enantiomérica (E) de 52), permitiendo obtener el enantiómero R del ácido mandélico con un exceso enantiomérico (ee) del 96%.

The development of methodologies for obtaining chiral compounds constitutes a major challenge on current chemistry. In this context, kinetic resolution catalyzed by lipases represents an excellent alternative. In homogeneous media, these enzymes display an equilibrium between two conformations (open and closed form), that can be displaced towards an open conformation (active form) in presence of hydrophobic supports. In this article lipase from Candida antarctica B (CAL-B) was purified and covalently immobilized onto Eupergit C epoxy supports (EC), Eupergit C activated with other functional groupsand octyl-agarose supports. These preparations of immobilized enzymes were used under different pH conditions in the kinetic resolution of (R,S)-methyl mandelate. In this study, EC-amino-CAL-B immobilized derivative was highlighted because it is highly enantioselective at pH 8 (enantiomeric ratio (E) of 52), allowing to obtain an R-enantiomer from mandelic acid with an enantiomeric excess (ee) of 96%.
Descritores: Biotransformação
Enzimas Imobilizadas
Engenharia de Proteínas
Responsável: CO56.3 - Biblioteca


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Id: lil-600567
Autor: Ospina S., Sonia Amparo.
Título: ¿Todo tiempo pasado fue mejor? Cómo ha incidido la tecnología enzimática en el bienestar de la humanidad: [editorial] / Does all the past was better? How has technology affected enzyme in human welfare
Fonte: Rev. colomb. biotecnol;13(1):5-7, jul. 2011.
Idioma: es.
Descritores: Engenharia de Proteínas/classificação
Engenharia de Proteínas/métodos
Engenharia de Proteínas/tendências
Tipo de Publ: Editorial
Responsável: CO326 - Departamento de Biología


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Id: lil-590326
Autor: García Quiroz, Felipe; Sinclair, S. Michael.
Título: Engineering antibody fragments: replicating the immune system and beyond: [review] / La ingeniería de fragmentos de anticuerpos: imitando y expandiendo el sistema inmune: [revisión]
Fonte: Rev. ing. bioméd;4(7):39-51, ene.- jun. 2010. ilus, tab.
Idioma: en.
Resumo: Since genetic engineering of humanized murine monoclonal antibodies was first demonstrated over two decades ago, antibody engineering technologies have evolved based upon an increasing understanding of the mechanisms involved in antibody generation in vivo, and a constant search for alternative routes to evolve and exploit the characteristics of antibodies. As a result, antibody engineers have devised innovative strategies for the rapid evolution and selection of antibodies and novel antibody designs (i.e., antibody fragments). Phage display, cell display and ribosome display technologies, which comprise the core of the currently available technologies for the discovery and preparation of such antibodies, are reviewed herein. This article intends to communicate the state-of-the-art technology available for the engineering of antibodies to a general readership interested in this important field. Therefore, important immunology concepts are introduced before detailed descriptions of the three antibody engineering technologies are presented in later sections. A comparison of these methodologies suggests that despite the predominance of phage display for the engineering of antibody fragments in the past 20 years, cell display and ribosome display will likely gain importance in the selection and discovery of the antibody fragments in the future. Finally, these technologies are likely to play an important role in the production of the next generation of antibody-based therapeutics.

Las tecnologías para la ingeniería de anticuerpos han evolucionado durante las últimas dos décadas, desde la demostración de la posibilidad de humanizar anticuerpos monoclonales de ratón mediante ingeniería genética, apoyadas en el creciente entendimiento de los mecanismos involucrados en la generación de anticuerpos in vivo, y en una búsqueda constante de rutas alternativas para evolucionar y explotar sus características. Es así como los ingenieros de anticuerpos han desarrollado estrategias innovadoras para la evolución y selección de anticuerpos y de novedosos diseños de anticuerpos conocidos como fragmentos de anticuerpos. Esta revisión se enfoca en tres tecnologías que comprenden el núcleo de las tecnologías actualmente disponibles para el descubrimiento y preparación de tales anticuerpos: la presentación en fagos, la presentación en células, y la presentación en ribosomas. Este artículo busca presentar el estado del arte de estas tecnologías a un grupo general de lectores interesados en este campo, por lo que inicialmente se introducen importantes conceptos de inmunología requeridos para comprender en detalle las tecnologías discutidas. Una comparación de estas metodologías para la ingeniería de anticuerpos sugiere que a pesar del dominio de las tecnologías basadas en la presentación en fagos durante los últimos 20 años, en los próximos años la presentación en células y la presentación en ribosomas probablemente ganarán importancia para la selección y descubrimiento de fragmentos de anticuerpos. Finalmente, es probable que estas tecnologías jueguen un papel importante en la producción de la siguiente generación de terapéuticos basados en anticuerpos.
Descritores: Fragmentos de Imunoglobulinas/biossíntese
Fragmentos de Imunoglobulinas/genética
Fragmentos de Imunoglobulinas/imunologia
Engenharia de Proteínas/tendências
Tipo de Publ: Revisão
Responsável: CO301.1 - Biblioteca Alberto Quevedo Diaz


  10 / 16 LILACS  
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Id: lil-448761
Autor: Sasseville, Maxime; St-Louis, Catherine; Khoudi, Habib; Beauregard, Marc.
Título: Controlling proteolytic degradation of the methionine enriched MB-1Trp protein
Fonte: Electron. j. biotechnol;7(3):04-05, Dec. 2004. ilus, graf, tab.
Idioma: en.
Resumo: Protein design is currently used for the creation of new proteins with desirable traits, which include a superior nutritional value. One of the challenges of protein design in this area is to achieve the production of stable native-like proteins that resist the proteolytic pressure of the organism used for its production (the bioreactor). We report here the identification of a specific peptide bond sensitive to E. coli proteolysis in the designer protein MB-1Trp. In an attempt to reduce proteolysis, we have created a MB-1TrpHis gene library in which the two amino acids surrounding the peptide bond, N44 and L45, were randomized using degenerated oligonucleotides. The initial characterization of MB-1TrpHis N44E/L45V and MB-1TrpHis N44E/L45M, 2 variants of the library that were more resistant than the parent protein, was performed in order to investigate the nature of the mutants' resistance. Our results suggest that the mutants behaved like MB-1Trp regarding folding and thermal stability, and that proteolytic resistance is due to the elimination of the protease recognition site.
Descritores: Aminoácidos Essenciais/genética
Aminoácidos Essenciais/metabolismo
Engenharia de Proteínas/métodos
Proteínas na Dieta/metabolismo
-Agroindústria
Reatores Biológicos
Biotecnologia
Dicroísmo Circular
Temperatura Alta
Mutação
Metionina/genética
Metionina/metabolismo
Desnaturação Proteica
Estrutura Secundária de Proteína
Responsável: CL1.1 - Biblioteca Central



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