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Id: biblio-1156206
Autor: Caetano, Edie Benedito; Vieira, Luiz Angelo; Sabongi Neto, João José; Caetano, Maurício Benedito Ferreira; Picin, Celis Piovesan; Silva Júnior, Luiz Claudio Nascimento da.
Título: Anatomical Study of the Motor Branches of the Radial Nerve in the Forearm / Estudo anatômico dos ramos motores do nervo radial no antebraço
Fonte: Rev. bras. ortop;55(6):764-770, Nov.-Dec. 2020. tab, graf.
Idioma: en.
Resumo: Abstract Objective To analyze the anatomical variations of the motor branches of the radial nerve in the elbow region. The origin, course, length, branches, motor points and relationships with neighboring structures were evaluated. Materials and Methods Thirty limbs from15 adult cadavers were dissected and prepared by intra-arterial injection of a 10% glycerin and formaldehyde solution. Results The first branch of the radial nerve in the forearm went to the brachioradialis muscle (BR), originating proximally to the division of the radial nerve into superficial branch of the radial nerve (SBRN) and posterior interosseous nerve (PIN) in all limbs. The branches to the extensor carpi radialis longus muscle (ECRL) detached from the proximal radial nerve to its division into 26 limbs, in 2, at the dividing points, in other 2, from the PIN. In six limbs, the branches to the BR and ECRL muscles originated from a common trunk. We identified the origin of the branch to the extensor carpi radialis brevis muscle (ECRB) in the PIN in 14 limbs, in the SBRN in 12, and in the radial nerve in only 4. The branch to the supinator muscle originated from the PIN in all limbs. Conclusion Knowledge of the anatomy of the motor branches of the radial nerve is important when performing surgical procedures in the region (such as the approach of the proximal third and the head of the radius, release of compressive syndromes of the posterior interosseous nerve and radial tunnel, and distal nerve transfers) in order to understand the order of recovery of muscle function after a nerve injury.

Resumo Objetivo Analisar as variações anatômicas dos ramos motores do nervo radial na região do cotovelo. Foram avaliadas a origem, curso, comprimento, ramificações, pontos motores e relações com estruturas vizinhas. Materiais e Métodos Foram dissecados 30 membros de 15 cadáveres adultos, preparados por injeção intra-arterial de uma solução de glicerina e formol a 10%. Resultados O primeiro ramo do nervo radial no antebraço foi para o músculo braquiorradial (BR), que se origina proximalmente à divisão do nervo radial em ramo superficial do nervo radial (RSNR) e nervo interósseo posterior (NIP) em todos os membros. Os ramos para o músculo extensor radial longo do carpo (ERLC) se desprenderam do nervo radial proximalmente à sua divisão em 26 membros, em 2, nos pontos de divisão, em outros 2, do NIP. Em seis, os ramos para os músculos BR e ERLC originavam-se de um tronco comum. Identificamos a origem do ramo para o músculo extensor radial curto do carpo (ERCC) no NIP em 14 membros, no RSNR em 12, e no nervo radial em apenas 4. O ramo para o músculo supinador originou-se do NIP em todos os membros. Conclusão O conhecimento da anatomia dos ramos motores do nervo radial é importante quando se realizam procedimentos cirúrgicos na região, como a abordagem do terço proximal e da cabeça do rádio, a liberação das síndromes compressivas do nervo interósseo posterior e do túnel radial, as transferências nervosas distais, e para entender a ordem de recuperação da função muscular após uma lesão nervosa.
Descritores: Nervo Radial
Rádio (Anatomia)
Procedimentos Cirúrgicos Operatórios
Punho
Cadáver
Transferência de Nervo
Marcação In Situ das Extremidades Cortadas
Cotovelo
Extremidades
Antebraço
Traumatismos do Antebraço
Glicerol
Cabeça
Anatomia
Injeções Intra-Arteriais
Responsável: BR26.1 - Biblioteca Central


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Id: biblio-983821
Autor: Maifrino, Laura Beatriz M; Lima, Nathalia E A de; Marques, Mara R; Cardoso, Clever G; Souza, Lidiane B de; Tomé, Tabata de Carvalho; Quintana, Hananiah Tardivo; Oliveira, Flavia de; Reis, Beatriz da Costa Aguiar Alves; Fonseca, Fernando Luiz Affonso.
Título: Evaluation of Collagen Fibers, MMP2, MMP9, 8-OHdG and Apoptosis in the Aorta of Ovariectomized LDL Knockout Mice Submitted to Aerobic Exercise / Avaliação de Fibras de Colágeno, MMP2, MMP9, 8-OH-Dg e Apoptose na Aorta de Ratas LDL Nocaute Ovariectomizadas Submetidas a Exercício Aeróbico
Fonte: Arq. bras. cardiol;112(2):180-188, Feb. 2019. graf.
Idioma: en.
Resumo: Abstract Background: In menopause, there is greater cellular exposure to oxidative stress, related to the decreased antioxidative effects of estrogen. These metabolic changes favor the progression of cardiovascular diseases, such as atherosclerosis. Abnormal function of the aorta - the most important artery - is associated with many cardiovascular diseases. Collagen, especially types I and III, is one of the most important aortic wall components and it can be affected by many factors, including menopause. The 8-OHdG is one of the main markers of DNA oxidative damage induced by reactive oxygen species (ROS). Objective: We aimed to investigate effects of moderate aerobic training on the ascending aorta of LDL-knockout (LDL-KO) and ovariectomized female mice. Methods: A total of 15 C57BL/6 mice and 15 LDL-KO mice were divided into experimental groups. The thickness and volume density of types I and III collagen fibers were performed by morphoquantitative analysis, whereas the MMP-2 and MMP-9 and 8-OHdG were detected by immunohistochemistry and apoptosis was detected by the TUNEL assay. The significance level for all tests was p < 0.05. Results: Exercise causes an increase in the thickness of the aorta in LDL-KO groups, particularly accentuated in the ovariectomized groups. The type I collagen fibers showed an increase in volume density influenced by training in both Control groups and in the LDL-KO group. Type III collagen density decreased in both groups. The MMP-2 showed moderade immunostaining in the tunica media in LDL-KO groups, which did not occur in the control groups and the MMP-9 stained irregularly in all tissues. The marker 8-OhdG was stronger in the exercise training groups. Additionally, the ovariectomy, the exercise training and the LDL-KO treatments increased apoptosis. Conclusion: These results suggest that moderate-intensity aerobic exercise in ovariectomized mice associated to an increase in LDL rate possibly increases oxidative stress and apoptosis induction.

Resumo Fundamento: Na menopausa, há maior exposição celular ao estresse oxidativo, relacionada à diminuição dos efeitos antioxidantes do estrogênio. Essas alterações metabólicas favorecem a progressão das doenças cardiovasculares, como a aterosclerose. A função anormal da aorta - a artéria mais importante - está associada a muitas doenças cardiovasculares. O colágeno, especialmente os tipos I e III, é um dos mais importantes componentes da parede da aorta e pode ser afetado por muitos fatores, incluindo a menopausa. Por sua vez, 8-OHdG é um dos principais marcadores de danos oxidativos do DNA induzidos por espécies reativas de oxigênio (EROS). Objetivo: Investigar os efeitos do treinamento aeróbico moderado na aorta ascendente de camundongos fêmeas, nocaute para LDL (LDL-KO) e ovariectomizadas. Métodos: Um total de 15 animais C57BL/6 e 15 animais LDL-KO foram divididos em grupos experimentais. A espessura e a densidade de volume das fibras de colágeno tipos I e III foram realizadas por análise morfoquantitativa; MMP-2 e MMP-9 e 8-OHdG foram detectadas por imunohistoquímica; e a apoptose foi detectada pelo ensaio TUNEL. O nível de significância adotado para todos os testes realizados foi p < 0,05. Resultados: o exercício causa aumento da espessura da aorta em grupos LDL-KO, particularmente acentuada em grupos ovariectomizados. As fibras de colágeno de tipo I mostraram aumento da densidade de volume influenciado pelo treinamento em animais controle e LDL-KO. A densidade do colágeno tipo III diminuiu em ambos os grupos. A MMP-2 mostrou imunomarcação moderada na túnica média em animais LDL-KO; em grupos controle, a MMP-9 marcou irregularmente em todos os tecidos. O marcador 8-OHdG foi mais forte nos grupos de treinamento de exercícios. Além disso, a ovariectomia, o treinamento físico e os tratamentos de LDL-KO aumentaram a apoptose. Conclusão: Esses resultados sugerem que exercícios aeróbicos de intensidade moderada em camundongos ovariectomizados associados ao aumento da taxa de LDL, possivelmente, aumentam o estresse oxidativo e a indução da apoptose.
Descritores: Aorta/metabolismo
Condicionamento Físico Animal/fisiologia
Ovariectomia
Colágeno/análise
Metaloproteinase 2 da Matriz/análise
Metaloproteinase 9 da Matriz/análise
-Aorta/patologia
Menopausa/metabolismo
Receptores de LDL/sangue
Imuno-Histoquímica
Túnica Média/patologia
Apoptose/fisiologia
Camundongos Knockout
Estresse Oxidativo/fisiologia
Marcação In Situ das Extremidades Cortadas
Comportamento Sedentário
Limites: Animais
Feminino
Ratos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-1011400
Autor: Sun, Deyu; Mu, Yi; Piao, Haozhe.
Título: MicroRNA-153-3p enhances cell radiosensitivity by targeting BCL2 in human glioma
Fonte: Biol. Res;51:56, 2018. graf.
Idioma: en.
Resumo: BACKGROUND: Glioma is the most prevalent malignant tumor in human central nervous systems. Recently, the development of resistance to radiotherapy in glioma patients markedly vitiates the therapy outcome. MiR-153-3p has been reported to be closely correlated with tumor progression, but its effect and molecular mechanism underlying radioresistance remains unclear in glioma. METHODS: The expression of miR-153-3p was determined in radioresistant glioma clinical specimens as well as glioma cell lines exposed to irradiation (IR) using quantitative real-time PCR. Cell viability, proliferation and apoptosis were then evaluated by MTT assay, colony formation assay, Flow cytometry analysis and caspase-3 activity assay in glioma cells (U87 and U251). Tumor forming was evaluated by nude mice model in vivo. TUNEL staining was used to detect cell apoptosis in nude mice model. The target genes of miR-153-3p were predicted and validated using integrated bioinformatics analysis and a luciferase reporter assay. RESULTS: Here, we found that miR-153-3p was down-regulated in radioresistant glioma clinical specimens as well as glioma cell lines (U87 and U251) exposed to IR. Enhanced expression of miR-153-3p promoted the radiosensitivity, promoted apoptosis and elevated caspase-3 activity in glioma cells in vitro, as well as the radiosensitivity in U251 cell mouse xenografs in vivo. Mechanically, B cell lymphoma-2 gene (BCL2) was identified as the direct and functional target of miR-153-3p. Moreover, restoration of BCL2 expression reversed miR-153-3p-induced increase of radiosensitivity, apoptosis and caspase-3 activity in U251 cells in vitro. In addition, clinical data indicated that the expression of miR-153-3p was significantly negatively associated with BCL2 in radioresistance of glioma samples. CONCLUSIONS: Our findings suggest that miR-153-3p is a potential target to enhance the effect of radiosensitivity on glioma cells, thus representing a new potential therapeutic target for glioma.
Descritores: Tolerância a Radiação/genética
Genes bcl-2/fisiologia
MicroRNAs/efeitos da radiação
MicroRNAs/fisiologia
Glioma/genética
-Fatores de Tempo
Regulação para Baixo
Regulação Neoplásica da Expressão Gênica
Sobrevivência Celular/efeitos da radiação
Western Blotting
Análise de Variância
Marcação de Genes/métodos
Genes bcl-2/efeitos da radiação
Marcação In Situ das Extremidades Cortadas
MicroRNAs/análise
Linhagem Celular Tumoral
Proliferação de Células/efeitos da radiação
Caspase 3/análise
Reação em Cadeia da Polimerase em Tempo Real
Citometria de Fluxo
Glioma/radioterapia
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Responsável: CL1.1 - Biblioteca Central


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Id: lil-742966
Autor: Souza, Celso Eduardo de; Pinter, Adriano; Donalisio, Maria Rita.
Título: Risk factors associated with the transmissionof Brazilian spotted fever in the Piracicaba river basin, State of São Paulo, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;48(1):11-17, jan-feb/2015. tab, graf.
Idioma: en.
Resumo: INTRODUCTION : Brazilian spotted fever (BSF) is a disease transmitted by ticks for which the etiological agent is Rickettsia rickettsii. The present essay evaluates the risk factors associated with the transmission of cases of BSF in the time period between 2003 and 2013 in the Piracicaba river basin, state of São Paulo. METHODS : This essay presents a retrospective study to identify the factors associated with the transmission of cases of BSF among all suspected cases identified by the System for Epidemiological Surveillance of São Paulo (CVE). After the description of temporal distribution (onset of symptoms) and the environmental and demographic variations of the confirmed and discarded cases, a multiple logistic regression model was applied. RESULTS : We searched 569 probable locations of infection (PLI) with 210 (37%) confirmed cases of BSF and 359 (63%) discarded cases. The associated variables for the confirmation of BSF in the multiple logistic model using a confidence interval (CI) of 95% were age (OR = 1.025 CI: 1.015-1.035), the presence of Amblyomma sculptum in the environment (OR = 1.629 CI: 1.097-2.439), the collection of ticks from horses (OR = 1.939 CI: 0.999-3.764), the presence of capybaras (OR = 1.467 CI: 1.009-2.138), an urban environment (OR = 1.515 CI: 1.036-2.231), and the existence of a dirty pasture (OR = 1.759 CI: 1.028-3.003). CONCLUSIONS : The factors associated with the confirmation of BSF cases included an urban environment, age, presence of the A. sculptum vector, the collection of ticks from horses, the presence of a capybara population, and a dirty pasture environment. .
Descritores: Apoptose/genética
Benzofuranos/uso terapêutico
-Apoptose/efeitos dos fármacos
Western Blotting
Linhagem Celular
Núcleo Celular/efeitos dos fármacos
Núcleo Celular/metabolismo
Eletroforese em Gel Bidimensional
Hemodinâmica/efeitos dos fármacos
Marcação In Situ das Extremidades Cortadas
Microscopia Eletrônica de Transmissão
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Infarto do Miocárdio/metabolismo
Miocárdio/citologia
Miocárdio/metabolismo
Miocárdio/ultraestrutura
Miócitos Cardíacos/citologia
Miócitos Cardíacos/metabolismo
NF-kappa B/metabolismo
Poli(ADP-Ribose) Polimerases/metabolismo
Ratos Wistar
Transdução de Sinais/efeitos dos fármacos
Transdução de Sinais/genética
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Espectrometria de Massas em Tandem
Limites: Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-1054927
Autor: Ozdemir, Ahmet; Bastug, Osman; Cilenk, Kubra T; Korkmaz, Levent; Korkut, Sabriye; Ozturk, Mehmet A; Sonmez, Mehmet F.
Título: ¿El licopeno puede eliminar los efectos perjudiciales de la hiperoxia en los cerebros inmaduros? / Can lycopene eliminate the harmful effects of hyperoxia in an immature brain?
Fonte: Arch. argent. pediatr;117(4):237-244, ago. 2019. ilus, tab.
Idioma: en; es.
Resumo: Objetivos: Al ser un antioxidante, el licopeno protege a las células contra el daño causado por los radicales libres, fortalece los enlaces intercelulares y mejora el metabolismo celular. Este estudio analiza los efectos del licopeno sobre los trastornos neurodegenerativos por hiperoxia en ratas recién nacidas a término. Métodos: Estas ratas se dividieron en cuatro grupos: grupo 1 de referencia con normoxia, grupo 2 con normoxia + licopeno, grupo 3 de referencia con hiperoxia y grupo 4 con hiperoxia + licopeno. Los grupos 1 y 2 se supervisaron en condiciones de aire ambiental, y los grupos 3 y 4 se supervisaron con un nivel de oxígeno > 85 % O2. Los grupos 2 y 4 recibieron inyecciones intraperitoneales de licopeno de 50 mg/kg/día; los otros grupos recibieron inyecciones intraperitoneales de aceite de maíz con el mismo volumen. Las ratas se sacrificaron en el día 11, después de 10 días con hiperoxia. Se extrajeron los cerebros, y se evaluaron los parámetros del sistema oxidativo. Resultados: Se detectaron lesiones cerebrales por hiperoxia en sustancia blanca, regiones corticales y tálamo. Aumentó la cantidad de células apoptóticas y disminuyó la cantidad de células PCNA positivas en los grupos 3 y 4, comparados con el grupo 1. No se observó una mejora significativa en la cantidad de células apoptóticas y células PCNA positivas en los grupos 3 y 4; además, aumentó la apoptosis. Conclusión: Se halló que el licopeno no mostró efectos terapéuticos para el daño cerebral en ratas recién nacidas. Además, se demostró que el licopeno podría causar efectos tóxicos.

Objectives. In addition to protecting cells against free radical harm thanks to its anti-oxidant nature, lycopene strengthens the bonds among cells and improves cell metabolism. This study focuses on analyzing therapeutic effects of lycopene in hyperoxia-induced neurodegenerative disorders in newborn rats. Methods. Term newborn rats were divided into four groups as the normoxia control group (group-1), normoxia+lycopene group (group-2), hyperoxia control group (group-3) and hyperoxia+lycopene group (group-4). Group-1 and group-2 were monitored in room air while the group-3 and group-4 were monitored at > 85% O2. The group-2 and group-4 were injected with lycopene intrapertioneally (i.p. ) at 50mg/kg/day while the other groups were injected with corn oil i.p. at the same volume. The rats we sacrificed on the 11th day following the 10-day hyperoxia. The brains were removed and oxidant system parameters were assessed. Results. Injury resulting from hyperoxia was detected in the white matter, cortical regions, and thalamus of the brains. It was observed that the number of apoptotic cells increased and the number of proliferating cell nuclear antigen (PCNA) positive cells decreased in the groups-3 and 4 compared to the group-1. No significant improvement in the number of apoptotic cells and PCNA positive cells was observed in the groups-3 and 4, and apoptosis increased as well. Conclusion. This study found that lycopene, did not show any therapeutic effects for brain damage treatment in newborn rats. In addition, this study demonstrated that lycopene might lead to toxic effects.
Descritores: Hiperóxia
Licopeno
-Ratos
Ensaio de Imunoadsorção Enzimática
Marcação In Situ das Extremidades Cortadas
Radicais Livres
Limites: Animais
Ratos
Responsável: AR94.1 - Centro de Información Pediatrica


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Id: biblio-1001091
Autor: Büyük, Başak; Demirci, Tuba; Adalı, Yasemen; Eroğlu, Hüseyin Avni.
Título: A new organ preservation solution for static cold storage of the liver. Amniotic fluid
Fonte: Acta cir. bras;34(4):e201900402, 2019. tab, graf.
Idioma: en.
Projeto: The Scientific and Technological Research Council of Turkey.
Resumo: Abstract Purpose: To evaluate the effect of amniotic fluid in liver preservation in organ transplantation, and compare it with standard preservation solutions. Methods: The groups consisted of Group 1: Ringer Lactate (RL) group, Group 2: HTK group, Group 3: UW group, Group 4: AF group. The livers of rats from Group 1, 2, 3, and 4 were perfused and placed into falcon tubes containing RL, HTK, UW, and AF solutions at +4‎°C, respectively. The tubes were stored for 12 hours in the refrigerator at +4°C. Tissue samples were taken at the 6th and 12th hours for histopathological examinations of the perfused livers, and storage solutions for biochemical analyzes at 6th and 12th hours. Results: AF was shown to maintain organ viability by reducing the number of cells undergoing apoptosis. Histopathological changes such as sinusoidal dilatation, hydropic degeneration, and focal necrosis were found to be similar to the groups in which the standard organ preservation solutions were used. Additionally, the results of INOS, IL-10, and TNF-α,which were evaluated immunohistochemically, have been shown to be similar to the UW and HTK groups. Conclusions: AF provided conservation similar to UW and HTK in the 12-hour liver SCS process. The fact that apoptosis values are comparable to standard preservation solutions supports the success of AF in the cold storage of the liver.
Descritores: Criopreservação/métodos
Soluções para Preservação de Órgãos/farmacologia
Líquido Amniótico
Fígado/irrigação sanguínea
Fígado/patologia
-Preservação de Órgãos/métodos
Cloreto de Potássio/farmacologia
Procaína/farmacologia
Valores de Referência
Fatores de Tempo
Sobrevivência de Tecidos
Imuno-Histoquímica
Traumatismo por Reperfusão/prevenção & controle
Distribuição Aleatória
Reprodutibilidade dos Testes
Fator de Necrose Tumoral alfa/análise
Interleucina-10/análise
Ratos Wistar
Marcação In Situ das Extremidades Cortadas
Óxido Nítrico Sintase Tipo II/análise
Solução de Ringer/farmacologia
Glucose/farmacologia
Manitol/farmacologia
Limites: Animais
Masculino
Tipo de Publ: Estudo Comparativo
Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-1054682
Autor: Li, Yanze; Wang, Lei; Chen, Zhiyuan; Liu, Xiuheng.
Título: Picroside II attenuates ischemia/reperfusion testicular injury by alleviating oxidative stress and apoptosis through reducing nitric oxide synthesis
Fonte: Acta cir. bras;34(11):e201901102, Nov. 2019. tab, graf.
Idioma: en.
Projeto: Application and Basic Research Project of Wuhan City; . Wuhan Morning Light Plan of Youth Science and Technology; . Natural Science Foundation of Hubei Province; . Research Project of Wuhan University.
Resumo: Abstract Purpose: To investigate the effect of Picroside II on testicular ischemia and reperfusion (l/R) injury and the underlying mechanism. Methods: Sprague-Dawley rats were randomly divided into 4 groups: sham operated group (Sham), Sham with Picroside II treatment group (Sham+ Pic II), l/R group (l/R) and l/R with Picroside II treatment group (I/R+ Pic II). l/R model was established by rotating the left testis 720° in a clock-wise direction for 4 hours. The histopathologic and spermatogenetic evaluation was performed. The apoptosis changes and the levels of HO-1 (heme oxygenase-1), MPO (myeloperoxidase), NOX (NADPH oxidase), SOD (superoxide dismutase), XO (xanthine oxidase) and NOS (nitric oxide synthase) were measured. Results: The seminiferous tubules were damaged in l/R rats, but Picroside II alleviated the changes induced by l/R. The increased level of apoptosis was decreased by Picroside II (P=0.01, 9.05±0.35 vs. 4.85±0.25). The activities of HO-1, MPO, NOX, XO and MDA content were increased and the SOD activity was decreased in l/R (P<0.05) and could be reversed by Picroside II (P=0.03, 405.5±7.5 vs. 304±17U/mgprot; P=0.02, 0.99±0.05 vs. 0.52±0.04 mgprot; P=0.01, 260+7 vs. 189±2 mgprot; P=0.04, 10.95+0.55 vs. 8.75+0.35 U/mgprot; P=0.045, 6.8+0.7 vs. 3.75+0.35 mgprot; P=0.04, 44.5+3.5 vs. 57.5+3.5 mgprot). Western blot showed that the expression of iNOS, nNOS and eNOS were increased in l/R (P<0.05); however, they were decreased after Picroside II treatment (P<0.05). Conclusion: Picroside II attenuated testicular I/R injury in rats mainly through suppressing apoptosis and oxidative stress through reduction of nitric oxide synthesis.
Descritores: Testículo/irrigação sanguínea
Traumatismo por Reperfusão/prevenção & controle
Cinamatos/farmacologia
Apoptose/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Glucosídeos Iridoides/farmacologia
Óxido Nítrico/biossíntese
-Traumatismo por Reperfusão/metabolismo
Traumatismo por Reperfusão/patologia
Distribuição Aleatória
Western Blotting
Ratos Sprague-Dawley
Peroxidase/análise
Marcação In Situ das Extremidades Cortadas
Heme Oxigenase-1/análise
Malondialdeído/análise
NADP/análise
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-787297
Autor: Cuevas-Gonzalez, Juan Carlos; Vega-Memíje, Maria Elisa; García-Vázquez, Francisco Javier; Aguilar-Urbano, Marco António.
Título: Detection of apoptosis in pemphigus vulgaris by TUNEL technique
Fonte: An. bras. dermatol;91(3):296-299graf.
Idioma: en.
Resumo: Abstract: Background: Pemphigus is part of a group of blistering diseases that affect the skin and mucous membranes. Based on its autoimmune origin, autoantibodies develop in pemphigus that are directed toward cell surface components of keratinocytes. However, some data cannot be explained, such as the lack of a relationship between autoantibody levels and the severity of clinical manifestations, treatment resistance, the presence of inflammatory infiltrates and the potential occurrence of apoptosis as determinants of vesicle formation. Objective: To examine the presence of apoptosis in pemphigus vulgaris by TUNEL technique. Methods: In this cross-sectional study, we selected 15 paraffin-embedded tissues from subjects who were diagnosed with pemphigus vulgaris by hematoxylin and eosin staining. The samples were subjected to TUNEL assay and examined under an Olympus BX61 fluorescence microscope. Positivity was categorized dichotomously, and the statistical analysis was performed using the X2 test. Results: Positivity was observed in basal layer cells in 14 (93.3%) cases. In 13 (86.7%) of the positive cases, we noted espinosum and granular layers that formed the blister roof, and in 12 cases (80%), positive acantholytic cells were observed. Conclusions: TUNEL positivity was observed in pemphigus vulgaris, implicating apoptosis in the pathophysiology of this condition, which can help guide the development of apoptotic blockers as therapeutics.
Descritores: Pênfigo/fisiopatologia
Apoptose/fisiologia
Marcação In Situ das Extremidades Cortadas/métodos
-Pele/fisiopatologia
Estudos Transversais
Acantólise/fisiopatologia
Vesícula/fisiopatologia
Pênfigo/patologia
Limites: Humanos
Adulto
Responsável: BR1.1 - BIREME


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Id: lil-787258
Autor: Yayi, Huang; Yeda, Xiao; Huaxin, Wang; Yang, Wu; Qian, Sun; Zhongyuan, Xia.
Título: Toll-like receptor 7 involves the injury in acute kidney ischemia/reperfusion of STZ-induced diabetic rats
Fonte: Acta cir. bras;31(7):448-455graf.
Idioma: en.
Resumo: ABSTRACT PURPOSE: To determine whether Toll-like receptor 7 (TLR7) is the potential targets of prevention or progression in the renal ischemia/reperfusion (I/R) injury of STZ-induced diabetic rats. METHODS: Thirty six Sprague-Dawley rats were randomly arranged to the nondiabetic (ND) or diabetic group (DM), with each group further divided into sham (no I/R injury), I/R (ischemia-reperfusion) and CD (given by Chloroquine) group. Preoperatively, Chloroquine (40 mg/kg, intraperitoneal injection.) was administrated 6 days for treatment group. I/R animals were subjected to 25 min of bilateral renal ischemia. Renal function, histology, apoptosis, cytokines, expression of TLR7, MyD88 and NF-κB were detected. RESULTS: The serum levels of blood urea nitrogen, creatinine, IL-6 and TNF-α, apoptotic tubular epithelial cells, expression of TLR7, MyD88 and NF-κB were significantly increased in DM+I/R group, compared with ND+I/R group (p<0.05). All these changes were further improved by TLR7 inhibition Chloroquine except Paller scores (p<0.05). CONCLUSION: Toll-like receptor 7 inhibition attenuates the acute renal ischemia/reperfusion injury of STZ-induced diabetic in SD rats.
Descritores: Traumatismo por Reperfusão/metabolismo
Diabetes Mellitus Experimental/metabolismo
Receptor 7 Toll-Like/metabolismo
Lesão Renal Aguda/metabolismo
Rim/metabolismo
-Traumatismo por Reperfusão/complicações
Distribuição Aleatória
NF-kappa B/metabolismo
Ratos Sprague-Dawley
Apoptose
Marcação In Situ das Extremidades Cortadas/métodos
Diabetes Mellitus Experimental/complicações
Modelos Animais de Doenças
Receptor 7 Toll-Like/sangue
Fator 88 de Diferenciação Mieloide/metabolismo
Lesão Renal Aguda/patologia
Rim/patologia
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-837712
Autor: Gao, Wenwei; Zhao, Bo; Liu, Lian; Yuan, Quan; Wu, Xiaojing; Xia, Zhongyuan.
Título: Myocardial ischemic post-conditioning protects the lung against myocardial ischemia/reperfusion-induced damage by activating GSK-3
Fonte: Acta cir. bras;32(5):376-387, May 2017. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Natural Science Foundation of Hubei Province.
Resumo: Abstract Purpose: To investigate whether modulating GSK-3β could attenuate myocardial ischemia reperfusion injury (MIRI) induced acute lung injury (ALI) and analyze the underlying mechanism. Methods: Male SD rats were subjected to MIRI with or without myocardial ischemic post-conditioning in the presence or absence of GSK-3β inhibitor. GSK-3β inhibitor was injected peritoneally 10min before MIRI. Lung W/D weight ratio, MPO, PMNs, histopathological changes, TUNEL, Bax, Bcl-2, IL-6, IL-8, IL-10, GSK-3β, and caspase-3 were evaluated in the lung tissues of all rats. Results: After MIRI, lung injury was significantly increased manifested as significant morphological changes and increased leukocytes in the interstitial capillaries, Lung W/D ratio, MPO, and PMN in BALF, which was associated with enhanced inflammation evidenced by increased expressions of IL-6, IL-8 and reduced expression of IL-10. MIRI significantly increased cell apoptosis in the lung as increased levels of apoptotosis, Bax, cleaved caspase-3, and reduced expression of Bcl-2 was observed, which was concomitant with reduced p-GSK-3β. All these changes were reversed/prevented by ischemic post-conditioning, while these beneficial effects of ischemic post-conditioning were abolished by GSK-3β inhibition. Conclusion: Myocardial ischemia reperfusion injury induces acute lung injury by induction of inflammation and cell apoptosis. Ischemic post-conditioning protects the lung from ALI following MIRI by increasing p-GSK-3β.
Descritores: Traumatismo por Reperfusão Miocárdica/prevenção & controle
Substâncias Protetoras/metabolismo
Lesão Pulmonar Aguda/prevenção & controle
Pós-Condicionamento Isquêmico/métodos
Glicogênio Sintase Quinase 3 beta/metabolismo
-Distribuição Aleatória
Regulação para Baixo
Interleucinas/metabolismo
Ratos Sprague-Dawley
Apoptose/efeitos dos fármacos
Peroxidase/metabolismo
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Substâncias Protetoras/farmacologia
Marcação In Situ das Extremidades Cortadas
Modelos Animais
Ativação Enzimática
Proteína X Associada a bcl-2/metabolismo
Caspase 3/metabolismo
Lesão Pulmonar Aguda/enzimologia
Glicogênio Sintase Quinase 3 beta/antagonistas & inibidores
Glicogênio Sintase Quinase 3 beta/farmacologia
Inflamação/metabolismo
Infarto do Miocárdio/patologia
Neutrófilos/enzimologia
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME



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