Base de dados : LILACS
Pesquisa : E05.393.731 [Categoria DeCS]
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Texto completo SciELO Saúde Pública
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Id: lil-756601
Autor: Temores-Alcántara, Guadalupe; Infante, César; Caballero, Marta; Flores-Palacios, Fátima; Santillanes-Allande, Nadia.
Título: Salud mental de migrantes centroamericanos indocumentados en tránsito por la frontera sur de México / Mental health of undocumented migrants in transit at the southern border of Mexico
Fonte: Salud pública Méx;57(3):227-233, may.-jun. 2015. ilus, tab.
Idioma: es.
Resumo: Objetivo. Conocer las necesidades percibidas de salud mental de migrantes centroamericanos indocumentados en tránsito por la ciudad de Tapachula, Chiapas. Material y métodos. Estudio cualitativo realizado en Casa de Migrantes de Tapachula, Chiapas. Se realizaron 20 entrevistas semiestructuradas a diez mujeres y diez hombres migrantes. Se exploró el estado de salud mental y las expectativas de atención. Se retomaron nociones teórico-metodológicas de la fenomenología sociológica. Resultados. Los migrantes presentaban signos y síntomas de daños en su salud mental relacionados con experiencias vividas en el lugar de origen y en el tránsito por México. La percepción sobre su salud mental es influida por el modelo biomédico hegemónico. Las expectativas de servicios se relacionaron con la satisfacción de necesidades básicas. Conclusiones. Es necesario fortalecer la respuesta del sistema de atención en salud mental a partir de estrategias de cooperación y emprender acciones que promuevan la superación de una construcción biomédica de salud mental que estigmatiza, medicaliza, segrega y dificulta el acceso a servicios.

Objective. To identify the perception and needs in mental health of Central American migrants in transit through Tapachula, Chiapas. Materials and methods. Qualitative study in a migrant shelter in Tapachula, Chiapas. In 20 semi-structured interviews with migrant men and women, we explored their perceptions on mental health and expectations on care. We used basic notions of phenomenology to guide the analysis. Results. Migrants had several mental health problems related to the conditions at their country of origin and due to their initial transit through Mexico.Their perception on mental health problems was heavily influenced by the biomedical health paradigm. The expectations they had on the provision of services were related to the satisfaction of basic needs. Conclusions. It is necessary to strengthen the governmental response to mental health needs through collaborative strategies. Also, actions are needed to further the understanding of mental health in order to transcend the biomedical notions that stigmatize, segregate and create a barrier to accessing services.
Descritores: Genética Reversa/métodos
Rhinovirus/genética
Rhinovirus/patogenicidade
-Clonagem Molecular
DNA Complementar/síntese química
Células HeLa/virologia
Reação em Cadeia da Polimerase/métodos
RNA Viral/isolamento & purificação
Rhinovirus/crescimento & desenvolvimento
Transfecção
Limites: Humanos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: lil-741314
Autor: Silva Jr., J.V.J.; Arenhart, S.; Santos, H.F.; Almeida-Queiroz, S.R.; Silva, A.N.M.R.; Trevisol, I.M.; Bertani, G.R.; Gil, L.H.V.G..
Título: Efficient assembly of full-length infectious clone of Brazilian IBDV isolate by homologous recombination in yeast
Fonte: Braz. j. microbiol;45(4):1555-1563, Oct.-Dec. 2014. ilus, graf, tab.
Idioma: en.
Resumo: The Infectious Bursal Disease Virus (IBDV) causes immunosuppression in young chickens. Advances in molecular virology and vaccines for IBDV have been achieved by viral reverse genetics (VRG). VRG for IBDV has undergone changes over time, however all strategies used to generate particles of IBDV involves multiple rounds of amplification and need of in vitro ligation and restriction sites. The aim of this research was to build the world's first VRG for IBDV by yeast-based homologous recombination; a more efficient, robust and simple process than cloning by in vitro ligation. The wild type IBDV (Wt-IBDV-Br) was isolated in Brazil and had its genome cloned in pJG-CMV-HDR vector by yeast-based homologous recombination. The clones were transfected into chicken embryo fibroblasts and the recovered virus (IC-IBDV-Br) showed genetic stability and similar phenotype to Wt-IBDV-Br, which were observed by nucleotide sequence, focus size/morphology and replication kinetics, respectively. Thus, IBDV reverse genetics by yeast-based homologous recombination provides tools to IBDV understanding and vaccines/viral vectors development.
Descritores: Recombinação Homóloga
Vírus da Doença Infecciosa da Bursa/genética
Genética Reversa/métodos
-Brasil
Células Cultivadas
Fibroblastos/virologia
Vetores Genéticos
Instabilidade Genômica
Vírus da Doença Infecciosa da Bursa/isolamento & purificação
Vírus da Doença Infecciosa da Bursa/fisiologia
Saccharomyces cerevisiae/genética
Transfecção
Cultura de Vírus
Replicação Viral
Limites: Animais
Embrião de Galinha
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: lil-697152
Autor: Santos, Jefferson Jose da Silva; Cordeiro, Marli Tenorio; Bertani, Giovani Rota; Marques, Ernesto Torres de Azevedo; Gil, Laura Helena Vega Gonzales.
Título: Construction and characterisation of a complete reverse genetics system of dengue virus type 3
Fonte: Mem. Inst. Oswaldo Cruz;108(8):983-991, 6/dez. 2013.
Idioma: en.
Projeto: FACEPE; . CNPq.
Resumo: Dengue virulence and fitness are important factors that determine disease outcome. However, dengue virus (DENV) molecular biology and pathogenesis are not completely elucidated. New insights on those mechanisms have been facilitated by the development of reverse genetic systems in the past decades. Unfortunately, instability of flavivirus genomes cloned in Escherichia coli has been a major problem in these systems. Here, we describe the development of a complete reverse genetics system, based on the construction of an infectious clone and replicon for a low passage DENV-3 genotype III of a clinical isolate. Both constructs were assembled into a newly designed yeast- E. coli shuttle vector by homologous recombination technique and propagated in yeast to prevent any possible genome instability in E. coli . RNA transcripts derived from the infectious clone are infectious upon transfection into BHK-21 cells even after repeated passages of the plasmid in yeast. Transcript-derived DENV-3 exhibited growth kinetics, focus formation size comparable to original DENV-3 in mosquito C6/36 cell culture. In vitro characterisation of DENV-3 replicon confirmed its identity and ability to replicate transiently in BHK-21 cells. The reverse genetics system reported here is a valuable tool that will facilitate further molecular studies in DENV replication, virus attenuation and pathogenesis.
Descritores: Vírus da Dengue/genética
Genética Reversa
RNA Viral/genética
Replicação Viral/genética
-Escherichia coli/genética
Vetores Genéticos/genética
Plasmídeos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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