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Pesquisa : E05.393.760.479 [Categoria DeCS]
Referências encontradas : 28 [refinar]
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Id: biblio-885980
Autor: BRAGA, Andrina Guimarães Silva; SOUZA, Krishna Ferri Alencar de; BARBIERI, Fabio da Silva; FERNANDES, Cleberson de Freitas; ROCHA, Rodrigo Barros; VIEIRA JUNIOR, José Roberto; LACERDA, Clariana Lins; CELESTINO, Caroline Oliveira; FACUNDO, Valdir Alves; BRITO, Luciana Gatto.
Título: Acaricidal activity of extracts from different structures of Piper tuberculatum against larvae and adults of Rhipicephalus microplus / Atividade acaricida de extratos de diferentes estruturas de Piper tuberculatum sobre larvas e adultos de Rhipicephalus microplus
Fonte: Acta amaz;48(1):57-62, Jan.-Mar. 2018. tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; . Empresa Brasileira de Pesquisa Agropecuária (Embrapa.
Resumo: ABSTRACT The strategies to control the cattle tick, Rhipicephalus microplus are based mainly on the use of synthetic pesticides. However, the emergence, establishment, and development of resistance of ticks is rendering the main chemical groups ineffective. Finding new molecules to effectively control infestations by R. microplus is necessary to maintain the productivity of cattle herds, particularly of taurine breeds established in equatorial and tropical regions of the world. Ethanol extracts from the leaves, stems, and fruits of Piper tuberculatum were evaluated in bioassays at concentrations of 50, 25, 12.50, 6.25, 3.12 and 1.56 mg mL-1. The concentrations lethal to 50% of the individuals (LC50) of tick larvae after 24 hours of exposure were 3.62, 3.99 and 5.30 mg mL-1 for fruit, stem and leaf extracts, respectively. Against the engorged females, the highest efficacy rates were obtained at the concentration of 50 mg mL-1, corresponding to 71.57%, 68.38% and 37.03% of the fruit, leaf and stem extracts, respectively. The main effect of the ethanol extracts was on the egg hatching rate of ticks, with a reduction of 55.63% for the fruit and leaf extracts, and 20.82% for the stem extract. The results show that P. tuberculatum is a promising source of molecules for use as active ingredients in pesticide formulations for R. microplus control.

RESUMO Estratégias de controle do carrapato dos bovinos, Rhipicephalus microplus, fundamentam-se na utilização de pesticidas. Os principais grupos químicos utilizados atualmente mostram-se ineficazes devido ao surgimento de populações resistentes. A pesquisa de novas moléculas com eficiência acaricida é uma necessidade para manutenção da produtividade dos rebanhos bovinos estabelecidos em regiões de clima tropical. Avaliamos a atividade de Piper tuberculatum para o controle de fêmeas ingurgitadas e larvas de R. microplus através de bioensaios de imersão de adultos e de pacotes de larvas. As concentrações avaliadas foram de 50; 25; 12,50; 6,25; 3,12 e 1,56 mg mL-1 de extrato etanólico de folha, talo e fruto de P. tuberculatum. As concentrações letais para 50% dos indivíduos (CL50) após 24 horas de exposição de larvas de R. microplus foram 3,62; 3,99 e 5,30 mg mL-1 para os extratos etanólicos de fruto, talo e folha, respectivamente. Para fêmeas ingurgitadas, a maior eficácia resultou da concentração de 50 mg mL-1 de extrato de fruto (71.57%). O principal efeito dos extratos etanólicos de P. tuberculatum foi sobre a eclodibilidade, com uma redução de 55.63% para extratos de fruto e folha. P. tuberculatum mostra-se promissora como fonte de moléculas candidatas a uso em pesticidas, em formulações destinadas ao controle das infestações de R. microplus.
Descritores: Anotação de Sequência Molecular
Responsável: BR6.1 - BCS - Biblioteca de Ciências da Saúde


  2 / 28 LILACS  
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Id: biblio-839392
Autor: Hong, Sung-Jun; Park, Gun-Seok; Khan, Abdur Rahim; Jung, Byung Kwon; Shin, Jae-Ho.
Título: Draft genome sequence of a caprolactam degrader bacterium: Pseudomonas taiwanensis strain SJ9
Fonte: Braz. j. microbiol;48(2):187-188, April.-June 2017.
Idioma: en.
Resumo: Abstract Pseudomonas taiwanensis strain SJ9 is a caprolactam degrader, isolated from industrial wastewater in South Korea and considered to have the potential for caprolactam bioremediation. The genome of this strain is approximately 6.2 Mb (G + C content, 61.75%) with 6,010 protein-coding sequences (CDS), of which 46% are assigned to recognized functional genes. This draft genome of strain SJ9 will provide insights into the genetic basis of its caprolactam-degradation ability.
Descritores: Pseudomonas/genética
Pseudomonas/metabolismo
DNA Bacteriano/genética
DNA Bacteriano/química
Caprolactama/metabolismo
Genoma Bacteriano
Análise de Sequência de DNA
-Pseudomonas/isolamento & purificação
Composição de Bases
Microbiologia da Água
Biotransformação
Fases de Leitura Aberta
Anotação de Sequência Molecular
Resíduos Industriais
Coreia (Geográfico)
Responsável: BR1.1 - BIREME


  3 / 28 LILACS  
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Id: biblio-839362
Autor: Park, Gun-Seok; Hong, Sung-Jun; Park, Seulki; Jin, Hyewon; Lee, Sang-Jae; Shin, Jae-Ho; Lee, Han-Seung.
Título: Draft genome sequence of alcohol-tolerant bacteria Pediococcus acidilactici strain K3
Fonte: Braz. j. microbiol;48(1):1-2, Jan.-Mar. 2017.
Idioma: en.
Projeto: Ministry of Education, Science and Technology.
Resumo: Abstract Pediococcus acidilactici strain K3 is an alcohol-tolerant lactic acid bacterium isolated from nuruk, which is a traditional Korean fermentation starter for makgeolli brewing. Draft genome of this strain was approximately 1,991,399 bp (G+C content, 42.1%) with 1525 protein-coding sequences (CDS), of which 44% were assigned to recognized functional genes. This draft genome sequence data of the strain K3 will provide insights into the genetic basis of its alcohol-tolerance.
Descritores: Adaptação Biológica/efeitos dos fármacos
Adaptação Biológica/genética
Genoma Bacteriano
Etanol/farmacologia
Pediococcus acidilactici/efeitos dos fármacos
Pediococcus acidilactici/genética
-Ácido Láctico/biossíntese
Biologia Computacional/métodos
Genômica/métodos
Etanol/metabolismo
Fermentação
Sequenciamento de Nucleotídeos em Larga Escala
Anotação de Sequência Molecular
Pediococcus acidilactici/isolamento & purificação
Pediococcus acidilactici/metabolismo
Responsável: BR1.1 - BIREME


  4 / 28 LILACS  
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Id: biblio-839346
Autor: Hong, Sung-Jun; Park, Chang Eon; Park, Gun-Seok; Kim, Min-Chul; Jung, Byung Kwon; Shin, Jae-Ho.
Título: Draft genome sequence of sulfur-reducing archaeon Thermococcus thioreducens DSM 14981T
Fonte: Braz. j. microbiol;48(1):3-4, Jan.-Mar. 2017.
Idioma: en.
Projeto: Ministry of Education.
Resumo: Abstract Thermococcus thioreducens DSM 14981T, a sulfur-reducing archaeon, was isolated from the rainbow hydrothermal vent site on the Mid-Atlantic Ridge. Herein, we report the draft genome sequence of T. thioreducens DSM 14981T; we obtained 41 contigs with a genome size of 2,052,483 bp and G + C content of 53.5%. This genome sequence will not only help understand how the archaeon adapts to the deep-sea hydrothermal environment but also aid the development of enzymes that are highly stable under extreme conditions for industrial applications.
Descritores: Enxofre/metabolismo
Thermococcus/genética
Thermococcus/metabolismo
Genoma Arqueal
Genômica
Sequenciamento de Nucleotídeos em Larga Escala
-Análise de Sequência de DNA
Biologia Computacional/métodos
Genômica/métodos
Anotação de Sequência Molecular
Responsável: BR1.1 - BIREME


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Id: biblio-839345
Autor: Moon, Ji-Hoi; Kim, Minjung; Lee, Jae-Hyung.
Título: Genome sequence of Prevotella intermedia SUNY aB G8-9K-3, a biofilm forming strain with drug-resistance
Fonte: Braz. j. microbiol;48(1):5-6, Jan.-Mar. 2017.
Idioma: en.
Projeto: Ministry of Health & Welfare, Republic of Korea; . Kyung Hee University.
Resumo: Abstract Prevotella intermedia has long been known to be as the principal etiologic agent of periodontal diseases and associated with various systemic diseases. Previous studies showed that the intra-species difference exists in capacity of biofilm formation, antibiotic resistance, and serological reaction among P. intermedia strains. Here we report the genome sequence of P. intermedia SUNY aB G8-9K-3 (designated ATCC49046) that displays a relatively high antimicrobial resistant and biofilm-forming capacity. Genome sequencing information provides important clues in understanding the genetic bases of phenotypic differences among P. intermedia strains.
Descritores: Genoma Bacteriano
Prevotella intermedia/efeitos dos fármacos
Prevotella intermedia/fisiologia
Biofilmes
Farmacorresistência Bacteriana
Sequenciamento de Nucleotídeos em Larga Escala
Antibacterianos/farmacologia
-Análise de Sequência de DNA
Biologia Computacional/métodos
Polimorfismo de Nucleotídeo Único
Genômica/métodos
Anotação de Sequência Molecular
Responsável: BR1.1 - BIREME


  6 / 28 LILACS  
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Id: biblio-839337
Autor: Ntushelo, Khayalethu; Mafofo, Joseph.
Título: Draft genome of a South African strain of Pectobacterium carotovorum subsp. brasiliense
Fonte: Braz. j. microbiol;48(1):11-12, Jan.-Mar. 2017.
Idioma: en.
Resumo: Abstract The draft genome of Pectobacterium carotovorum subsp. brasiliense (Pcb) which causes blackleg of potato was submitted to the NCBI and released with reference number NZ_LGRF00000000.1. The estimated genome size based on the draft genome assembly is 4,820,279 bp from 33 contigs ranging in length from 444 to 1,660,019 nucleotides. The genome annotation showed 4250 putative genes, 4114 CDS and 43 pseudo-genes. Three complete rRNA gene species were detected: nine 5S, one 16S and one 23S. Other partial rRNA gene fragments were also identified, nine 16S rRNA and three 23S rRNA. A total of 69 tRNA genes and one ncRNA gene were also annotated in this genome.
Descritores: Genoma Bacteriano
Pectobacterium carotovorum/genética
Genômica
-Análise de Sequência de DNA
Biologia Computacional/métodos
Genômica/métodos
Sequenciamento de Nucleotídeos em Larga Escala
Anotação de Sequência Molecular
Responsável: BR1.1 - BIREME


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Id: biblio-839336
Autor: Petkauskaite, Raimonda; Blom, Jochen; Goesmann, Alexander; Kuisiene, Nomeda.
Título: Draft genome sequence of pectic polysaccharide-degrading moderate thermophilic bacterium Geobacillus thermodenitrificans DSM 101594
Fonte: Braz. j. microbiol;48(1):7-8, Jan.-Mar. 2017. tab.
Idioma: en.
Projeto: “TermozymOS’ project of the National Research Programme “Healthy and Safe Food” by the Lithuanian Science Council.
Resumo: Abstract Geobacillus thermodenitrificans DSM 101594 was isolated as a producer of extracellular thermostable pectic polysaccharide degrading enzymes. The completely sequenced genome was 3.6 Mb in length with GC content of 48.86%. A number of genes encoding enzymatic active against the high molecular weight polysaccharides of potential biotechnological importance were identified in the genome.
Descritores: Genoma Bacteriano
Genômica
Geobacillus/genética
Sequenciamento de Nucleotídeos em Larga Escala
-Pectinas/metabolismo
Biologia Computacional/métodos
Genômica/métodos
Geobacillus/metabolismo
Anotação de Sequência Molecular
Responsável: BR1.1 - BIREME


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Id: biblio-839335
Autor: Delamuta, Jakeline Renata Marçon; Ribeiro, Renan Augusto; Gomes, Douglas Fabiano; Souza, Renata Carolini; Chueire, Ligia Maria Oliveira; Hungria, Mariangela.
Título: Genome sequence of Bradyrhizobium embrapense strain CNPSo 2833T, isolated from a root nodule of Desmodium heterocarpon
Fonte: Braz. j. microbiol;48(1):9-10, Jan.-Mar. 2017.
Idioma: en.
Projeto: Embrapa; . CNPq.
Resumo: Abstract Bradyrhizobium embrapense CNPSo 2833T is a nitrogen-fixing symbiont of the legume pasture Desmodium. Its draft genome contains 8,267,832 bp and 7876 CDSs. The symbiotic island includes nodulation and nitrogen fixation genes resembling the operon organization of B. japonicum. Several CDSs related to secretion proteins and stress tolerance were also identified.
Descritores: Genoma Bacteriano
Bradyrhizobium/genética
Genômica
Nódulos Radiculares de Plantas/microbiologia
Fabaceae/microbiologia
-Simbiose
Análise de Sequência de DNA
Biologia Computacional/métodos
Bradyrhizobium/isolamento & purificação
Bradyrhizobium/metabolismo
Genômica/métodos
Sequenciamento de Nucleotídeos em Larga Escala
Anotação de Sequência Molecular
Responsável: BR1.1 - BIREME


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Id: biblio-1017082
Autor: Mei, Lanju; Dong, Na; Li, Fosheng; Li, Na; Yao, Min; Chen, Fang; Tang, Lin.
Título: Transcriptome analysis of female and male flower buds of Idesia polycarpa Maxim. var. vestita Diels
Fonte: Electron. j. biotechnol;29:39-46, sept. 2017. ilus, tab, graf.
Idioma: en.
Projeto: Science and Technology support projects of Sichuan Province.
Resumo: Background: Idesia polycarpa Maxim. var. vestita Diels, a dioecious plant, is widely used for biodiesel due to the high oil content of its fruits. However, it is hard to distinguish its sex in the seedling stage, which makes breeding and production problematic as only the female tree can produce fruits, and the mechanisms underlying sex determination and differentiation remain unknown due to the lack of available genomic and transcriptomic information. To begin addressing this issue, we performed the transcriptome analysis of its female and male flower. Results: 28,668,977 and 22,227,992 clean reads were obtained from the female and male cDNA libraries, respectively. After quality checks and de novo assembly, a total of 84,213 unigenes with an average length of 1179 bp were generated and 65,972 unigenes (78.34%) could be matched in at least one of the NR, NT, Swiss-Prot, COG, KEGG and GO databases. Functional annotation of the unigenes uncovered diverse biological functions and processes, including reproduction and developmental process, which may play roles in sex determination and differentiation. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed many unigenes annotated as metabolic pathways, biosynthesis of secondary metabolites pathways, plant­ pathogen interaction, and plant hormone signal transduction. Moreover, 29,953 simple sequence repeats were identified using the microsatellite software. Conclusion: This work provides the first detailed transcriptome analysis of female and male flower of I. polycarpa and lays foundations for future studies on the molecular mechanisms underlying flower bud development of I. polycarpa.
Descritores: Reprodução/genética
Salicaceae/genética
Transcriptoma
-Análise de Sequência de RNA
Genes de Plantas
Repetições de Microssatélites
Salicaceae/crescimento & desenvolvimento
Bases de Dados Genéticas
Sequenciamento de Nucleotídeos em Larga Escala
Anotação de Sequência Molecular
Responsável: CL1.1 - Biblioteca Central


  10 / 28 LILACS  
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Id: biblio-1015852
Autor: Yan, Kuan; Wei, Qin; Feng, Ruizhang; Zhou, Wanhai; Chen, Fang.
Título: Transcriptome analysis of Cinnamomum longepaniculatum by high-throughput sequencing
Fonte: Electron. j. biotechnol;28:58-66, July. 2017. tab, graf, ilus.
Idioma: en.
Projeto: Sichuan Education Department; . Innovative Program of Sichuan Undergraduates; . Key Lab of Aromatic Plant Resources Exploitation and Utilization in Sichuan Higher Education.
Resumo: Background: Cinnamomum longepaniculatum is an important commercial crop and the main source of volatile terpenoids. The biosynthesis of key bioactive metabolites of C. longepaniculatum is not well understood because of the lack of available genomic and transcriptomic information. To address this issue, we performed transcriptome sequencing of C. longepaniculatum leaves to identify factors involved in terpenoid metabolite biosynthesis. Results: Transcriptome sequencing of C. longepaniculatum leaves generated over 56 million raw reads. The transcriptome was assembled using the Trinity software and yielded 82,061 unigenes with an average length of 879.43 bp and N50 value of 1387 bp. Furthermore, Benchmarking Universal Single-Copy Orthologs analysis indicated that our assembly is 91% complete. The unigenes were used to query the nonredundant database depending on sequence similarity; 42,809 unigenes were homologous to known genes in different species, with an annotation rate of 42.87%. The transcript abundance and Gene Ontology analyses revealed that numerous unigenes were associated with metabolism, while others were annotated in functional categories including transcription, signal transduction, and secondary metabolism. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that 19,260 unigenes were involved in 385 metabolic pathways, with 233 unigenes found to be involved in terpenoid metabolism. Moreover, 23,463 simple sequence repeats were identified using the microsatellite identification tool. Conclusion: This is the first detailed transcriptome analysis of C. longepaniculatum. The findings provide insights into the molecular basis of terpenoid biosynthesis and a reference for future studies on the genetics and breeding of C. longepaniculatum.
Descritores: Terpenos/metabolismo
Cinnamomum/genética
Sequenciamento de Nucleotídeos em Larga Escala
Transcriptoma
-Transcrição Genética
Cruzamento
Óleos Voláteis/metabolismo
Repetições de Microssatélites
Anotação de Sequência Molecular
Ontologia Genética
Responsável: CL1.1 - Biblioteca Central



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