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Id: biblio-1283791
Autor: González Bonet, Ileana; Romero Elías, María Jacqueline; Morales Mejías, Erik; Valdez Moyano, Eliana; Cofré Loyola, Cecilia; Manques Maldonado, Belarmino; Rojas Rubio, Armando.
Título: Patrón EPIYA en cepas de Helicobacter pylori CagA positivas en pacientes del Hospital Regional de Talca / EPIYA pattern in Helicobacter pylori CagA positive strains in patients of the Regional Hospital of Talca
Fonte: Rev. méd. Maule;33(1):8-13, jun. 2017. tab.
Idioma: es.
Resumo: BACKGROUND: The clinical outcome of Helicobacter pylori (H. pylori) infection has been related to the presence of CagA protein. This protein is highly polymorphic and its oncogenic ability depends on the number and type of tyrosine phosphorylation sites in the EPIYAs repeat sequences (A, B, C and D). AIM: To determine the EPIYA patterns of the CagA gene in H. pylori strains and its relationship with gastrointestinal pathology in infected patients of the Regional Hospital of Talca. MATERIAL AND METHODS: The strains were isolated from gastric biopsies and characterized by bacteriological and molecular methods. Gastrointestinal pathology was characterized by histopathological analysis. For the determination of the presence of the cagA gene and the EPIYAs standards, the conventional PCR technique was used. RESULTS: 138 DNA samples from H. pylori strains were analyzed. 92.0% (127/138) of the isolates carried the cagA gene, of which 66 (52.0%) corresponded to the EPIYA-ABC pattern, 43 (33.8%) to the EPIYA-ABCC pattern and 21 16.5%) to the EPIYA-ABCCC phosphorylation pattern. 50.4% (64/127) of cagA positive strains isolated from dyspeptic patients in the Maule region have more than two C sites of phosphorylation. The number of EPIYAs C motifs was associated with the presence of more severe histopathological damage in the gastric mucosa.
Descritores: Neoplasias Gástricas/microbiologia
Neoplasias Gástricas/patologia
Helicobacter pylori/genética
Infecções por Helicobacter/microbiologia
Infecções por Helicobacter/patologia
Motivos de Aminoácidos
-Neoplasias Gástricas/epidemiologia
Proteínas de Bactérias/genética
Biópsia
DNA Bacteriano/genética
DNA Bacteriano/química
Chile/epidemiologia
Epidemiologia Descritiva
Endoscopia do Sistema Digestório
Infecções por Helicobacter/epidemiologia
Comissão de Ética
Análise de Sequência de DNA
Antígenos de Bactérias/genética
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Responsável: CL1.1 - Biblioteca Central


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Id: lil-777450
Autor: Carvalho, Isabel Azevedo; Schwarz, David Germano Gonçalves; Pietralonga, Pricila Aparecida Grasse; Faria, Ana Carolina Silva; Braga, Isis Freitas Espechit; Carvalho, Gabriel Domingos; Valente, Fabrício Luciani; Machado, João Paulo; Guimarães, Lize Maciel Pinheiro; Ferrari, Maria de Lourdes Abreu; Silva Júnior, Abelardo; Moreira, Maria Aparecida Scatamburlo.
Título: Presence of Mycobacterium avium subsp. paratuberculosis (MAP) in Brazilian patients with inflammatory bowel diseases and in controls / Presença de Mycobacterium avium subsp. paratuberculosis (MAP) em pacientes brasileiros com doença inflamatória intestinal e em controles
Fonte: Säo Paulo med. j;134(1):13-19, Jan.-Feb. 2016. tab, graf.
Idioma: en.
Resumo: CONTEXT AND OBJECTIVE: Mycobacterium avium subsp. paratuberculosis (MAP) has attracted the interest of researchers because of similarities between paratuberculosis and Crohn's disease (CD). The aim of this study was to evaluate the frequency of MAP through cultures, histology and polymerase chain reaction (PCR) on intestinal biopsies from Brazilian CD patients. Quantitative real time PCR (qRT-PCR) was performed on positive samples. DESIGN AND SETTING: Analytical cross-sectional study with control group at two federal universities. METHODS: Fresh samples were collected from 25 patients; five with CD, eight with ulcerative colitis (UC) and 12 controls with non-inflammatory bowel disease (nIBD). Formalin-fixed paraffin-embedded (FFPE) samples from 143 patients were also collected: 44 CD, 49 UC and 56 nIBD. RESULTS: None of the fresh samples was positive for MAP. Five FFPE samples (one CD, two UC and two nIBD) and three fresh samples (one in each group) were positive through IS900-PCR. qRT-PCR was performed on these eight samples. Among the FFPE samples, there were 192.12 copies/μl in the CD group, 72.28 copies/μl in UC and 81.43 copies/μl in nIBD. Among the fresh samples, there were 432.99 copies/μl, 167.92 copies/μl and 249.73 copies/μl in the CD, UC and nIBD groups, respectively. The highest bacterial load was in the CD group. CONCLUSION: This study does not provide evidence for a role of MAP in the etiology of CD, although MAP DNA was detected in all three patient groups. This is the first report of MAP presence in human intestinal biopsies in Brazil.

CONTEXTO E OBJETIVO: Mycobaterium avium subsp. paratuberculosis (MAP) tem atraído o interesse de pesquisadores devido às semelhanças entre a paratuberculose e a doença de Crohn (CD). Este estudo objetivou avaliar a frequência de MAP por meio de cultura, histologia e reação da polimerase em cadeia (PCR), em biópsias intestinais de pacientes brasileiros com CD. PCR quantitativa em tempo real (qRT-PCR) foi realizada nas amostras positivas. TIPO DE ESTUDO E LOCAL: Estudo transversal analítico com grupo controle realizado em duas universidades federais. MÉTODOS: Amostras frescas foram coletadas de 25 pacientes; cinco com CD, oito com colite ulcerativa (UC) e 12 controles sem doença inflamatória intestinal (nIBD). Também foram coletadas 149 amostras fixadas em parafina (FFPE): 44 CD, 49 UC e 56 nIBD. RESULTADOS: Nenhuma das amostras frescas foi positiva para MAP. Cinco amostras FFPE (uma CD, duas UC e duas nIBD) e três amostras frescas (uma de cada grupo) foram positivas por IS900-PCR. qRT-PCR foi realizada nessas oito amostras. Nas amostras FFPE, havia 192,12 cópias/μl no grupo CD, 72,28 cópias/μl no UC e 81,43 cópias/μl no nIBD. Nas amostras frescas, havia 432,99 cópias/μl, 167,92 cópias/μl e 249,73 cópias/μl nos grupos CD, UC e nIBD, respectivamente. A maior carga bacteriana foi encontrada no grupo CD. CONCLUSÃO: Este estudo não fornece evidências do papel de MAP na etiologia da CD, embora DNA de MAP tenha sido detectado em pacientes dos três grupos. Este é o primeiro relato da presença de MAP em biópsias intestinais humanas no Brasil.
Descritores: Doenças Inflamatórias Intestinais/microbiologia
Colite Ulcerativa/microbiologia
Doença de Crohn/microbiologia
Mycobacterium avium subsp. paratuberculosis/isolamento & purificação
-Estudos de Casos e Controles
Reação em Cadeia da Polimerase/métodos
Estudos Transversais
Mycobacterium avium subsp. paratuberculosis/patogenicidade
Análise de Sequência de DNA/métodos
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Criança
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-1132668
Autor: Damé-Teixeira, Naile; Parolo, Clarissa Cavalcanti Fatturi; Malz, Marisa; Devine, Deirdre Ann; Do, Thuy.
Título: Gene expression profile of Scardovia spp. in the metatranscriptome of root caries
Fonte: Braz. oral res. (Online);34:e042, 2020. tab, graf.
Idioma: en.
Projeto: Leeds Teaching Hospitals Charitable Foundation; . Dunhill Medical Trust; . Brazilian National Counsel of Technological and Scientific Development; . Coordination for the Improvement of Higher Level Education; . Rio Grande do Sul State Foundation for Research Support.
Resumo: Abstract A few investigations of caries biofilms have identified Scardovia spp.; however, little is known about its involvement in caries pathogenesis. The purpose of this study was to assess the gene expression profile of Scardovia spp. in root caries, and compare it with other microorganisms. Clinical samples from active root caries lesions were collected. Microbial mRNA was isolated and cDNA sequenced. The function and composition of the Scardovia were investigated using two methods: a) de novo assembly of the read data and mapping to contigs, and b) reads mapping to reference genomes. Pearson correlation was performed (p < 0.05). Proportion of Scardovia inopinata and Scardovia wiggsiae sequences ranged from 0-6% in the root caries metatranscriptome. There was a positive correlation between the transcriptome of Lactobacillus spp. and Scardovia spp. (r = 0.70; p = 0.03), as well as with other Bifidobacteriaceae (r = 0.91; p = 0.0006). Genes that code for fructose 6-phosphate phosphoketolase (the key enzyme for "Bifid shunt"), as well as ABC transporters and glycosyl-hydrolases were highly expressed. In conclusion, "Bifid shunt" and starch metabolism are involved in carbohydrate metabolism of S. inopinata and S. wiggsiae in root caries. There is a positive correlation between the metabolism abundance of Lactobacillus spp., Bifidobacteriaceae members, and Scardovia in root caries.
Descritores: Expressão Gênica
Actinobacteria/genética
Cárie Radicular/microbiologia
-Valores de Referência
DNA Bacteriano
Mapeamento Cromossômico
Actinobacteria/isolamento & purificação
Análise de Sequência de DNA
Estatísticas não Paramétricas
Biofilmes
Perfilação da Expressão Gênica
Transcriptoma
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Adulto
Idoso
Idoso de 80 Anos ou mais
Responsável: BR1.1 - BIREME


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Id: biblio-1279484
Autor: Cerqueira, Najila Nolie Catarine Dantas; Rotundo, Matheus Marcos; Marceniuk, Alexandre Pires; Cruz, Vanessa Paes da; Foresti, Fausto; Oliveira, Claudio.
Título: Molecular identification of Brachygenys and Haemulon species (Perciformes: Haemulidae) from the Brazilian coast
Fonte: Neotrop. ichthyol;19(2):e200109, 2021. tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico; . Conselho Nacional de Desenvolvimento Científico e Tecnológico; . Fundação de Amparo à Pesquisa do Estado de São Paulo; . Fundação Amazônia Paraense de Amparo a Estudos e Pesquisas; . Programa de Treinamento Institutional; . Programa de Treinamento Institutional.
Resumo: The fishes of the Haemulidae family are currently allocated to 19 genera with a worldwide distribution in the tropical and subtropical waters of the world's oceans. Brachygenys and Haemulon are important genera of reef fish in Brazil, as they occur in large shoals, which are both ecologically and commercially valuable. This study identified the Brazilian species of the genera Brachygenys and Haemulon using DNA barcodes. While we found only a single lineage in Brachygenys chrysargyrea, Haemulon melanurum, H. parra, and H. squamipinna, more than one molecular operational taxonomic unit (MOTU) was identified in H. atlanticus, H. aurolineatum, and H. plumieri, indicating the possible existence of discrete populations or cryptic species.(AU)

Os peixes da família Haemulidae estão atualmente distribuídos em 19 gêneros, com distribuição mundial em águas oceânicas tropicais e subtropicais. Brachygenys e Haemulon são importantes gêneros de peixes recifais do Brasil, visto que ocorrem em grandes cardumes, de valores ecológicos e comerciais. Este estudo identificou as espécies brasileiras dos gêneros Brachygenys e Haemulon usando o código de barras de DNA. Embora apenas uma única linhagem de Brachygenys chrysargyrea, Haemulon melanurum, H. parra e H. squamipinna tenha sido encontrada em nosso conjunto de dados, mais de uma unidade taxonômica operacional molecular (MOTU) foi identificada em H. atlanticus, H. aurolineatum e H. plumieri, indicando a possível existência de populações discretas ou espécies crípticas.(AU)
Descritores: Perciformes
Distribuição de Produtos
Biologia Molecular
-Análise de Sequência de DNA
Peixes
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-838974
Autor: Valenzuela, Carlos Y.
Título: Selective intra-dinucleotide interactions and periodicities of bases separated by K sites: a new vision and tool for phylogeny analyses
Fonte: Biol. Res;50:3, 2017. tab, graf.
Idioma: en.
Resumo: Direct tests of the random or non-random distribution of nucleotides on genomes have been devised to test the hypothesis of neutral, nearly-neutral or selective evolution. These tests are based on the direct base distribution and are independent of the functional (coding or non-coding) or structural (repeated or unique sequences) properties of the DNA. The first approach described the longitudinal distribution of bases in tandem repeats under the Bose-Einstein statistics. A huge deviation from randomness was found. A second approach was the study of the base distribution within dinucleotides whose bases were separated by 0, 1, 2... K nucleotides. Again an enormous difference from the random distribution was found with significances out of tables and programs. These test values were periodical and included the 16 dinucleotides. For example a high ¨positive¨ (more observed than expected dinucleotides) value, found in dinucleotides whose bases were separated by (3K + 2) sites, was preceded by two smaller ¨negative¨ (less observed than expected dinucleotides) values, whose bases were separated by (3K) or (3K + 1) sites. We examined mtDNAs, prokaryote genomes and some eukaryote chromosomes and found that the significant non-random interactions and periodicities were present up to 1000 or more sites of base separation and in human chromosome 21 until separations of more than 10 millions sites. Each nucleotide has its own significant value of its distance to neutrality; this yields 16 hierarchical significances. A three dimensional table with the number of sites of separation between the bases and the 16 significances (the third dimension is the dinucleotide, individual or taxon involved) gives directly an evolutionary state of the analyzed genome that can be used to obtain phylogenies. An example is provided.
Descritores: Filogenia
Sequência de Bases/genética
Genoma
Análise de Sequência de DNA/métodos
Nucleotídeos/genética
-Periodicidade
Células Procarióticas/química
Valores de Referência
Algoritmos
DNA Mitocondrial/genética
Distribuição de Qui-Quadrado
Colágeno/genética
HIV-1/genética
Evolução Molecular
Sequências de Repetição em Tandem
Estruturas Cromossômicas
Deriva Genética
Drosophila melanogaster/genética
Epistasia Genética/genética
Nucleotídeos/química
Limites: Humanos
Animais
Responsável: CL1.1 - Biblioteca Central


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Id: lil-792801
Autor: Shahraki-Zahedani, Shahram; Rigi, Shahnaz; Bokaeian, Mohammad; Ansari-Moghaddam, Alireza; Moghadampour, Mehdi.
Título: First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran
Fonte: Rev. Soc. Bras. Med. Trop;49(4):441-445, July-Aug. 2016. tab, graf.
Idioma: en.
Resumo: Abstract: INTRODUCTION: Extended-spectrum beta-lactamases (ESBLs) are bacterial enzymes capable of hydrolyzing beta-lactams. The aim of this study was to describe the prevalence of TEM- and SHV-type ESBL-producing Klebsiella pneumoniae strains in Zahedan, Southeast Iran. METHODS: A total of 170 non-repetitive K. pneumoniae strains were collected from patients referred to three teaching hospitals of Zahedan. Antibiotic susceptibility testing was determined for 17 antibiotics using the Kirby-Bauer disc diffusion method. The frequency of ESBL-producing strains was calculated, and minimum inhibitory concentrations of ESBL-producing strains were determined for cefotaxime, ceftazidime, ceftriaxone, and cefpodoxime. The presence of bla TEM and bla SHV genes was tested in all ESBL-producing strains using polymerase chain reaction and DNA sequencing. RESULTS: Among the 170 K. pneumoniae clinical isolates, 55 (32.4%) were ESBL producers; 92.7% (n=51) and 72.7% (n=40) of the isolates carried the bla SHV and bla TEM genes, respectively, and 67.3% (n=37) carried both genes. The sequencing results showed that all bla TEM types were bla TEM-1, except for two isolates that were bla TEM-104. The bla SHV types were bla SHV-1, bla SHV-11, bla SHV-12, bla SHV-99, bla SHV-108, and bla SHV-110. CONCLUSIONS: The percentage of bla TEM and bla SHV among ESBL-producing K. pneumoniae isolates from Zahedan is relatively high, indicating the need for further surveillance and consideration in antibiotic use. To the best of our knowledge, this is the first report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-type ESBLs among clinical isolates of K. pneumoniae from Iran, and TEM-1, SHV-1, SHV-11, and SHV-12 appear to be the dominant ESBLs in this region.
Descritores: Proteínas de Bactérias/genética
beta-Lactamases/biossíntese
DNA Bacteriano/genética
Klebsiella pneumoniae/efeitos dos fármacos
Klebsiella pneumoniae/genética
Antibacterianos/farmacologia
-Fenótipo
Infecções por Klebsiella/microbiologia
Análise de Sequência de DNA
Farmacorresistência Bacteriana Múltipla/genética
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Genes Bacterianos
Genótipo
Irã (Geográfico)
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-897038
Autor: Silva Júnior, Valdemir Vicente da; Ferreira, Laura Durão; Alves, Lílian Rodrigues; Cabral, Adriane Borges; Jácome, Paula Regina Luna de Araújo; Araújo, Paulo Sérgio Ramos de; Lopes, Ana Catarina de Souza; Maciel, Maria Amélia Vieira.
Título: Detection of multidrug-resistant Pseudomonas aeruginosa harboring bla GES-1 and bla GES-11 in Recife, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;50(6):764-768, Nov.-Dec. 2017. tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Abstract INTRODUCTION: Pseudomonas aeruginosa, an important pathogen globally, presents several resistance mechanisms. This study aimed to investigate the presence of bla GES in clinical isolates of Pseudomonas aeruginosa obtained from various clinical specimens from patients admitted to three different hospitals in Recife, Brazil. The Guiana extended spectrum beta-lactamase (GES) enzymes are responsible for conferring broad spectrum resistance to beta-lactam drugs, including the carbapenems. METHODS: A total of 100 carbapenem-resistant P. aeruginosa isolates underwent polymerase chain reaction (PCR) testing to identify bla GES, bla KPC, bla SPM-1, bla IMP, and bla VIM. Additionally, PCR products positive for bla GES were sequenced. The clonal profiles of these same isolates were then determined by means of enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis. RESULTS: PCR analysis revealed that four isolates harbored bla GES; DNA sequencing showed that two harbored bla GES-1 and two bla GES-11. Beta-lactamase genes bla SPM-1, bla IMP, bla VIM, and bla KPC were investigated; none of these genes was detected. Automated susceptibility testing methods (Vitek®2, bioMérieux) showed that the bla GES-1-positive isolates were only susceptible to polymyxin B. The patterns obtained with ERIC-PCR methods showed clonal relationship between the two isolates that harbored bla GES-11, whereas different clonal profiles were found in the isolates harboring bla GES-1. CONCLUSIONS: We detected the presence of bacterial isolates positive for two different variants of the enzyme GES in three different hospitals from Recife, Brazil. These enzymes have a great capacity for dissemination among Gram-negative bacteria and confer broad-spectrum resistance to beta-lactam antibiotics and to the carbapenems.
Descritores: Pseudomonas aeruginosa/genética
beta-Lactamases/genética
Farmacorresistência Bacteriana Múltipla/genética
Antibacterianos/farmacologia
-Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/enzimologia
beta-Lactamases/efeitos dos fármacos
Brasil
Sequência de Bases
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-959424
Autor: García, José; Martínez, Dianny; Caña, Luisa; González, Diorelis; Rodríguez, Lucy; Rodulfo, Hectorina; Donato, Marcos De; Guzmán, Militza.
Título: Genes qnr en Enterobacteriaceae aisladas en un hospital de Venezuela / qnr genes in Enterobacteriaceae isolated from at a hospital in Venezuela
Fonte: Rev. chil. infectol;35(2):147-154, abr. 2018. tab, graf.
Idioma: es.
Projeto: Misión Ciencia.
Resumo: Resumen Introducción: La resistencia de enterobacterias a quinolonas se ha difundido por el mundo, fenómeno presente también en Venezuela. El mecanismo de esta resistencia pudiera estar mediado por genes incluidos en el cromosoma bacteriano o transmitirse en el interior de plásmidos. Objetivo: Evaluar la resistencia a quino-lonas, codificada por genes qnr, presentes en cepas de enterobacterias, aisladas en el Hospital Universitario de Cumaná, Venezuela. Métodos: A las cepas obtenidas se les realizaron pruebas de susceptibilidad antimicrobiana a quinolonas, β-lactámicos y aminoglucósidos. La presencia del gen qnr se determinó por RPC. Las enterobacterias portadoras del gen qnr fueron sometidas al proceso de conjugación bacteriana para comprobar su capacidad de transferencia. A las transconjugantes obtenidas se les realizó pruebas de susceptibilidad antimicrobiana y RPC para comprobar la transferencia de los genes. Resultados: Se encontraron elevados porcentajes de resistencia antimicrobiana a quinolonas y betalactámicos. El 33,6% de las cepas eran portadoras del gen qnrB, y 0,9% del gen qnrA. Se obtuvieron 23 cepas transconjugantes; de éstas, 20 portaban el gen qnrB, no se observó la presencia de qnrA. Discusión: En conclusión, el elevado porcentaje de genes qnr encontrado en las enterobacterias aisladas, y comprobada la presencia de éstos en plásmidos transferibles, complica la aplicación de tratamientos basados en quinolonas y fluoroquinolonas, por lo que es recomendable el uso racional de estos antimicrobianos, y proponer la rotación de la terapia antimicrobiana, a fin de evitar la selección de cepas resistentes.

Background: Enterobacteria resistant to quinolones is increasing worldwide, including Venezuela. The mechanism for this resistance could be due to genes included in the chromosome or in transmissible plasmids. Aim: To evaluate the resistance to quinolones, coded by qnr genes present in enterobacteria species, isolated in the University Hospital of Cumana, Venezuela. Methods: Antimicrobial susceptibility tests to quinolones, beta-lactams and aminoglycosides were carried out to all the isolates. The presence of qnr genes were determined by PCR. The isolates carrying the qnr genes were used for bacterial conjugation tests to determine the presence of transferable plasmids. Antimicrobial susceptibility tests and PCR were carried out in the transconjugants to verify the transfer of the genes. Results: High levels of antimicrobial resistance to quinolones and beta-lactams were found among the isolates. We found that 33.6% of the isolates carry the qnrB gene and 0.9% qnr A gene. Of the 23 transconjugants, 20 showed to have qnrB gene, but none qnrA. Discussion: We concluded that the high frequency of qnr genes found in the enterobacteria isolates and their presence on transferable plasmids, complicate the use of quinolones for the treatment of bacterial infections, thus, a treatment plan should be designed with the rational use and the rotation of different types of antimicrobials, in order to avoid the selection of increasingly resistant strains.
Descritores: Plasmídeos
Quinolonas/farmacologia
Resistência beta-Lactâmica/genética
Farmacorresistência Bacteriana/genética
Enterobacteriaceae/genética
Infecções por Enterobacteriaceae/genética
Bactérias Gram-Negativas/genética
Antibacterianos/farmacologia
-Venezuela
beta-Lactamases/genética
DNA Bacteriano/genética
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Proteínas de Escherichia coli
Enterobacteriaceae/isolamento & purificação
Infecções por Enterobacteriaceae/microbiologia
Genes Bacterianos
Bactérias Gram-Negativas/classificação
Hospitais Universitários
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950759
Autor: Fakruddin, Md; Rahaman, Mizanur; Ahmed, Monzur Morshed; Hoque, Md Mahfuzul.
Título: Stress tolerant virulent strains of Cronobacter sakazakii from food
Fonte: Biol. Res;47:1-12, 2014. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The aim of this study was to isolate and characterize virulent strains of Cronobacter sakazakii from food samples of Bangladesh. RESULT: Six (6) Cronobacter sakazakii was isolated and identified from 54 food samples on the basis of biochemical characteristics, sugar fermentation, SDS-PAGE of whole cell protein, plasmid profile and PCR of Cronobacter spp. specific genes (esak, gluA, zpx, ompA, ERIC, BOX-AIR) and sequencing. These strains were found to have moderately high antibiotic resistance against common antibiotics and some are ESBL producer. Most of the C. sakazakii isolates were capable of producing biofilm (strong biofilm producer), extracellular protease and siderophores, curli expression, haemolysin, haemagglutinin, mannose resistant haemagglutinin, had high cell surface hydrophobicity, significant resistance to human serum, can tolerate high concentration of salt, bile and DNase production. Most of them produced enterotoxins of different molecular weight. The isolates pose significant serological cross-reactivity with other gram negative pathogens such as serotypes of Salmonella spp., Shigella boydii, Shigella sonnei, Shigella flexneri and Vibrio cholerae. They had significant tolerance to high temperature, low pH, dryness and osmotic stress. CONCLUSION: Special attention should be given in ensuring hygiene in production and post-processing to prevent contamination of food with such stress-tolerant virulent Cronobacter sakazakii.
Descritores: Estresse Fisiológico/fisiologia
Cronobacter sakazakii/fisiologia
Leite/microbiologia
Microbiologia de Alimentos
-Bangladesh
Virulência
DNA Bacteriano/análise
Resistência a Tetraciclina/genética
Reação em Cadeia da Polimerase/métodos
Especiarias/microbiologia
Sideróforos/metabolismo
Análise de Sequência de DNA
Primers do DNA
Reações Cruzadas
Cronobacter sakazakii/isolamento & purificação
Cronobacter sakazakii/classificação
Cronobacter sakazakii/patogenicidade
Leite/classificação
Eletroforese em Gel de Poliacrilamida
Fermentação/fisiologia
Temperatura Alta
Concentração de Íons de Hidrogênio
Antibacterianos/farmacologia
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1041560
Autor: Scavuzzi, Alexsandra Maria Lima; Firmo, Elza Ferreira; Oliveira, Érica Maria de; Lopes, Ana Catarina de Souza.
Título: Emergence of bla NDM-1 associated with the aac(6')-Ib-cr, acrB, cps, and mrkD genes in a clinical isolate of multi-drug resistant Klebsiella pneumoniae from Recife-PE, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;52:e20180352, 2019. tab.
Idioma: en.
Resumo: Abstract INTRODUCTION: The emergence of New Delhi metallo-β-lactamase (NDM) is concernig because it reduces the antibiotic therapy options for bacterial infections. METHODS: Resistant and virulent genes from an isolate of Klebsiella pneumoniae derived from a patient with sepsis in a hospital in Recife-PE, Brazil, were investigated using PCR and DNA sequencing. RESULTS: bla NDM-1, aac(6')-Ib-cr and acrB resistance genes, and cps and mrkD virulence genes were detected. CONCLUSIONS To our knowledge, this is the first report on bla NDM-1 in Recife-PE. This detection alerts researchers to the need to control the spread of bla NDM-1 resistance gene by this bacterium in Brazil.
Descritores: Proteínas de Bactérias/genética
Virulência/genética
beta-Lactamases/genética
Farmacorresistência Bacteriana Múltipla/genética
Klebsiella pneumoniae/genética
Antibacterianos/farmacologia
-Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Sepse/microbiologia
Klebsiella pneumoniae/efeitos dos fármacos
Klebsiella pneumoniae/enzimologia
Limites: Humanos
Feminino
Responsável: BR1.1 - BIREME



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