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Id: biblio-1116300
Autor: Contreras, Roberto; van den Brink, Liesbeth; Sepúlveda, Bernardo; Aguayo, Fernanda; Porcile, Vincenzo.
Título: Phylogenetic relationships of plant species from the flowering desert of the Atacama Region / Relaciones filogenéticas de especies de plantas que crecen en el desierto florido de la Región de Atacama
Fonte: Bol. latinoam. Caribe plantas med. aromát;19(3):300-313, mayo 2020. ilus, tab.
Idioma: en.
Projeto: Regional Innovation Fund for Regional Competitiveness; . Regional Government of Atacama; . National Forestry Corporation.
Resumo: Every 3 to 7 year angiosperms species of the flowering desert appear in the Atacama Region of Chile, as a result of the climatic phenomenon "El Niño". Our objective was to evaluate the universality of matK and rbcL barcode markers of these species, and validate their taxon through phylogenetic relationships. Argemone hunnemannii, Oenothera coquimbensis, Malesherbia humilis, Leucocoryne appendiculata, Loasa elongata, Nicotiana solanifolia, Stachys grandidentata, Aristolochia chilensis, Alstroemeria kingii and Adesmia eremophila, almost all classified as endemic to Chile, were collected in Pan de Azúcar and Llanos de Challe National Park (Atacama Region, Chile) at the end of October 2017. The phylogeny of these ten angiosperm species from the flowering desert was analyzed using rbcL and matK markers with the maximum likelihood and Bayesian inference methods. The results showed that 70% of the species can be distinguished with the matK or rbcL locus, however, 100% were distinguished using both loci. The phylogenetic results showed that the species formed clades with high reliability and high support with both the matK and rbcL genes, when comparing our results with sequences obtained from GenBank. The matK and rbcL genes are efficient markers for analyzing phylogenetic relationships and validating the taxonomy of flowering species.

Las especies de angiospermas del Desierto Florido de la Región de Atacama de Chile aparecen cada 3 a 7 años, influenciado por el fenómeno climático "El Niño". Nuestro objetivo fue evaluar la universalidad de los marcadores de código de barra matK y rbcL de estas especies, y validar su taxón por medio de relaciones filogenéticas. Las especies Argemone hunnemannii, Oenothera coquimbensis, Malesherbia humilis, Leucocoryne appendiculata, Loasa elongata, Nicotiana solanifolia, Stachys grandidentata, Aristolochia chilensis, Alstroemeria kingii y Adesmia eremophila son clasificadas la mayoría endémicas de Chile. Estas especies fueron colectadas en el Parque Nacional Pan de Azúcar y Llanos de Challe, Región de Atacama, Chile. La colecta se realizó a fines de octubre de 2017. Con los marcadores rbcL y matK se analizó la filogenia con los métodos máxima verosimilitud e inferencia bayesiana en diez especies de angiosperma del Desierto Florido. Los resultados mostraron que el 70% de las especies pueden ser distinguidas con un locus matK o rbcL, sin embargo, el 100% se distinguió usando ambos locus. Los resultados filogenéticos mostraron que las especies formaron clados con alta fiabilidad y alto soporte tanto con los genes matK y rbcL, al comparar con accesos de secuencias obtenidas de GenBank. Lo genes matK y rbcL son marcadores eficientes para analizar relaciones filogenéticas y validar el taxón de las especies de flor.
Descritores: Filogenia
Plantas/genética
Deserto
Código de Barras de DNA Taxonômico/métodos
-Ribulose-Bifosfato Carboxilase
Chile
Análise de Sequência de DNA
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Chile
Texto completo
Id: biblio-950877
Autor: Sun, Yufang; Liu, Dianfeng; Xiao, Bo; Jiang, Guofang.
Título: The comparative mitogenomics and phylogenetics of the two grouse-grasshoppers (Insecta, Orthoptera, Tetrigoidea)
Fonte: Biol. Res;50:34, 2017. tab, graf.
Idioma: en.
Projeto: National (Youth) Natural Science Foundation of China; . Youth Natural Science Foundation of Jiangsu Province.
Resumo: OBJECTIVE: This study aimed to reveal the mitochondrial genomes (mtgenomes) of Tetrix japonica and Alulatettix yunnanensis, and the phylogenetics of Orthoptera species. METHODS: The mtgenomes of A. yunnanensis and T. japonica were firstly sequenced and assembled through partial sequences amplification, and then the genome organization and gene arrangement were analyzed. Based on nucleotide/amino acid sequences of 13 protein-coding genes and whole mtgenomes, phylogenetic trees were established on 37 Orthoptera species and 5 outgroups, respectively. RESULTS: Except for a regulation region (A+T rich region), a total of 37 genes were found in mtgenomes of T. japonicaand A. yunnanensis, including 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes, which exhibited similar characters with other Orthoptera species. Phylogenetic tree based on 13 concatenated protein-coding nucleotide sequences were considered to be more suitable for phylogenetic reconstruction of Orthoptera species than amino acid sequences and mtgenomes. The phylogenetic relationships of Caelifera species were Acridoidea and Pamphagoidea > Pyrgomorphoidea > Pneumoroidea > Eumastacoidea > Tetrigoidea > Tridactyloidea. Besides, a sister-group relationship between Tettigonioidea and Rhaphidophoroidea was revealed in Ensifera. CONCLUSION: Concatenated protein-coding nucleotide sequences of 13 genes were suitable for reconstruction of phylogenetic relationship in orthopteroid species. Tridactyloidea was a sister group of Tetrigoidea in Caelifera, and Rhaphidophoroidea was a sister group of Tettigonioidea in Ensifera.
Descritores: Evolução Molecular
Genoma Mitocondrial/genética
Gafanhotos/genética
-Filogenia
Sequência de Bases
Análise de Sequência de DNA
Gafanhotos/classificação
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1160804
Autor: Blanco, Antonio; Montamat, Enrique E.
Título: Caracterización de aislamientos de Trypanosoma Cruzi: posible importancia médica / Characterization of isolation of Trypanosoma Cruzi: possible medical importance
Fonte: Bol. Acad. Nac. Med. B.Aires;(supl):49-70, jul. 1992. tab, graf.
Idioma: es.
Conferência: Apresentado em: Homenaje al Doctor Salvador Mazza en el 50º aniversario de su fallecimiento, Buenos Aires, 15 oct. 1996.
Descritores: Análise de Sequência de DNA
Eletroforese
Heterogeneidade Genética
Trypanosoma cruzi/imunologia
Trypanosoma cruzi/isolamento & purificação
Vetores Genéticos/isolamento & purificação
-Cardiomiopatia Chagásica/diagnóstico
Testes Sorológicos
Limites: Animais
Humanos
Responsável: AR1.1 - Biblioteca Rafael Herrera Vegas


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Id: biblio-1160778
Autor: Copelli, Silvia.
Título: Tema 2: endocrinología infantil. Estudio molecular en niños con anomalías de la diferenciación sexual / Molecular study in children with sexual differentiation anomalies
Fonte: Bol. Acad. Nac. Med. B.Aires;(supl):323-8, jul. 1992.
Idioma: es.
Conferência: Apresentado em: Reunión Conjunta, 18 y Sesión Científica, 2, Montevideo, 10-11 oct. 1996.
Descritores: Análise de Sequência de DNA
Biologia Molecular
Transtornos do Desenvolvimento Sexual/diagnóstico
Transtornos do Desenvolvimento Sexual/genética
Transtornos do Desenvolvimento Sexual/terapia
-Disgenesia Gonadal/diagnóstico
Síndrome de Turner
Transtornos do Desenvolvimento Sexual
Limites: Masculino
Feminino
Humanos
Pré-Escolar
Responsável: AR1.1 - Biblioteca Rafael Herrera Vegas


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Texto completo SciELO Brasil
Texto completo
Id: lil-794563
Autor: Zuo, Hong-Peng; Guo, Ying-Yu; Che, Lin; Wu, Xian-Zheng.
Título: Hypomethylation of Interleukin-6 Promoter is Associated with the Risk of Coronary Heart Disease / Hipometilação do Promotor de Interleucina-6 Associada com Risco de Doença Arterial Coronariana
Fonte: Arq. bras. cardiol;107(2):131-136, Aug. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Background: Interleukin-6 (IL-6) is implicated in the pathogenesis of coronary heart disease (CHD), and IL-6 expression has associated with reduced DNA methylation of its gene promoter. However, there are no data on IL-6 promoter methylation and the risk of CHD. Objective: To examine whether IL-6 promoter methylation measured in blood leukocyte DNA is associated with CHD risk. Methods: A total of 212 cases with CHD and 218 controls were enrolled. Methylation at two CpG sites in IL-6 promoter was measured by bisulfite pyrosequencing, and the mean IL-6 methylation was calculated by averaging the methylation measures of the two CpGs. Results: Mean methylation level in IL-6 promoter in CHD cases was significantly lower than that in controls (p = 0.023). Logistic regression analysis showed that IL-6 methylation was inversely associated with the risk of CHD. The odds ratios (ORs) of CHD for subjects in the second and first (lowest) tertile of IL-6 methylation were 1.87 (95% CI = 1.10‑3.20) and 2.01 (95% CI = 1.19-3.38) (ptrend = 0.013), respectively, compared to subjects in the third (highest) tertile. The IL-6 hypomethylation-related risk estimates tended to be stronger for acute myocardial infarction (ptrend = 0.006). CpG position-specific analysis showed that hypomethylation of position 1 conferred a more pronounced increase in CHD risk than that of position 2. Conclusion: These findings suggest that DNA hypomethylation of IL-6 promoter is associated with the increased risk for CHD, especially for acute myocardial infarction. The two distinct CpGs in IL-6 may contribute differently to the development of CHD.

Resumo Fundamento: Interleucina-6 (IL-6) está implicada na patogênese de doença arterial coronariana (DAC), sendo sua expressão associada com redução da metilação de DNA do promotor do seu gene. Entretanto, não há dados sobre metilação do promotor de IL-6 e risco de DAC. Objetivo: Verificar se a metilação do promotor de IL-6 medida no DNA de leucócitos sanguíneos acha-se associada com risco de DAC. Métodos: este estudo arrolou 212 casos com DAC e 218 controles. Metilação em dois sítios de CpG no promotor de IL-6 foi medida por pirosequenciamento de bissulfito, sendo a metilação média de IL-6 calculada pela média das medidas de metilação dos dois CpGs. Resultados: A média do nível de metilação no promotor de IL-6 nos casos de DAC foi significativamente mais baixa do que nos controles (p = 0,023). Análise de regressão logística mostrou associação inversa entre metilação de IL-6 e risco de DAC. As razões de chance (OR) de DAC para indivíduos no segundo e no primeiro (mais baixo) tercis de metilação de IL-6 foram 1,87 (IC 95%: 1,10-3,20) e 2,01 (IC 95%: 1,19-3,38) (ptrend = 0,013), respectivamente, comparadas à de indivíduos no terceiro (mais alto) tercil. As estimativas de risco relacionado à hipometilação de IL-6 tenderam a ser mais fortes para infarto agudo do miocárdio (ptrend = 0,006). Análise com especificidade de posição de CpG mostrou que hipometilação na posição 1 conferiu maior elevação no risco de DAC do que na posição 2. Conclusão: Tais achados sugerem que a hipometilação de DNA do promotor de IL-6 está associada com elevado risco de DAC, especialmente para infarto agudo do miocárdio. Os dois CpGs distintos no promotor de IL-6 podem contribuir de modo diferente para o desenvolvimento de DAC.
Descritores: Interleucina-6/genética
Regiões Promotoras Genéticas
Ilhas de CpG
Metilação de DNA
Doença das Coronárias/genética
Predisposição Genética para Doença/genética
-Interleucina-6/metabolismo
Análise de Sequência de DNA
Angina Instável/genética
Infarto do Miocárdio/genética
Limites: Humanos
Masculino
Feminino
Idoso
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
Texto completo
Id: biblio-1011408
Autor: Márquez, Sebastián L; Blamey, Jenny M.
Título: Isolation and partial characterization of a new moderate thermophilic Albidovulum sp. SLM16 with transaminase activity from Deception Island, Antarctica
Fonte: Biol. Res;52:5, 2019. tab, graf.
Idioma: en.
Resumo: BACKGROUND: A moderately thermophilic, slightly halophilic, aerobic, Gram-stain negative, bacterial strain, SLM16, was isolated from a mixed of seawater-sand-sediment sample collected from a coastal fumarole located in Whalers Bay, Deception Island, Antarctica. The aim was to screen for thermophilic microorganisms able to degrade primary amines and search for amine transaminase activity for potential industrial application. RESULTS: Identification and partial characterization of the microorganism SLM16 were carried out by means of morphological, physiological and biochemical tests along with molecular methods. Cells of strain SLM16 were non-motile irregular rods of 1.5-2.5 µm long and 0.3-0.45 µm wide. Growth occurred in the presence of 0.5-5.5% NaCl within temperature range of 35-55 °C and pH range of 5.5-9.5, respectively. The DNA G+C composition, estimated from ftsY gene, was 66% mol. Phylogenetic analysis using de 16S rRNA gene sequence showed that strain SLM16 belongs to the marine bacterial genus Albidovulum. CONCLUSION: Strain SLM16 is a moderate thermophilic Gram negative microorganisms which belongs to the marine bacterial genus Albidovulum and is closely related to Albidovulum inexpectatum species based on phylogenetic analysis. Additionally, amine-transaminase activity towards the arylaliphatic amine α-methylbenzylamine was detected.
Descritores: Água do Mar/microbiologia
DNA Bacteriano/genética
Rhodobacteraceae/isolamento & purificação
Rhodobacteraceae/enzimologia
Transaminases/metabolismo
-Filogenia
RNA Ribossômico 16S/genética
Técnicas de Tipagem Bacteriana
Análise de Sequência de DNA
Rhodobacteraceae/classificação
Regiões Antárticas
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1130108
Autor: Tomaseto, Alex Augusto; Alpiste, Marcel Costa; Nassar, Alessandra Figueiredo de Castro; Destéfano, Suzete Aparecida Lanza.
Título: Antibacterial activity of phytopathogenic Streptomyces strains against bacteria associated to clinical diseases / Atividade antimicrobiana de Streptomyces fitopatogênicas contra bactérias associadas a doenças de importância clínica
Fonte: Arq. Inst. Biol;87:e0142020, 2020. tab.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil; . Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Resumo: The genus Streptomyces is associated with the ability to produce and excrete a variety of bioactive compounds, such as antibiotic, antifungal and antiviral. Biological active polyketide and peptide compounds with applications in medicine, agriculture and biochemical research are synthesized by PKS-I and NRPS genes. The evaluation of the presence of these genes associated with the biosynthesis of secondary metabolites in different phytopathogenic Streptomyces strains were performed using degenerated primers. The positive signal was observed in 58/63 Streptomyces strains for NRPS gene, 43/63 for PKS-I, and for PKS-II all the 63 strains showed positive signal of amplification. These strains also were tested with double layer agar-well technique against bacterial with clinical importance, and it was possible to observe the Streptomyces spp. strains were able to inhibit the growth of 14, 20, 13 and 3 isolates Gram-positive and Gram-negative bacteria, Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 14579), Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 11775) respectively. The Streptomyces sp. strains IBSBF 2019 and IBSBF 2397 showed antibacterial activity against all four bacteria-target tested.(AU)

O gênero Streptomyces apresenta alta capacidade de produzir e excretar uma grande variedade de compostos biologicamente ativos, como antibióticos, antifúngicos e antivirais. Compostos biologicamente ativos de policetídeos e peptídeos com aplicações na medicina, agricultura e pesquisas bioquímicas são sintetizados pelos genes PKS-I e NRPS. A avaliação da presença desses genes associados à biossíntese de metabólitos secundários em diferentes linhagens de Streptomyces fitopatogênicas foi realizada através do uso de primers degenerados. O sinal positivo foi observado em 58/63 linhagens de Streptomyces para o gene NRPS, 43/63 para o gene PKS-I e, para o gene PKS-II, todas as 63 linhagens apesentaram o sinal positivo de amplificação. Essas linhagens também foram testadas através da técnica de dupla camada contra bactérias de importância clínica e foi possível observar que as linhagens de Streptomyces spp. foram capazes de inibir o crescimento de 14, 20, 13 e 3 isolados de bactérias Gram-positivas e Gram-negativas, Staphylococcus aureus (ATCC 25923), Bacillus cereus (ATCC 14579), Pseudomonas aeruginosa (ATCC 27853) e Escherichia coli (ATCC 11775), respectivamente. As linhagens de Streptomyces sp. ISBSF 2019 e 2397 apresentaram atividade antibacteriana contra todas as bactérias-alvo testadas.(AU)
Descritores: Pseudomonas aeruginosa/crescimento & desenvolvimento
Staphylococcus aureus/crescimento & desenvolvimento
Streptomyces/metabolismo
Bacillus cereus/crescimento & desenvolvimento
Escherichia coli/crescimento & desenvolvimento
Antibacterianos/metabolismo
-Peptídeo Sintases/genética
Streptomyces/genética
Amplificação de Genes
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Primers do DNA
Policetídeo Sintases/genética
Antibacterianos/farmacologia
Responsável: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Id: biblio-839184
Autor: Campana, Eloiza Helena; Xavier, Danilo Elias; Petrolini, Fernanda Villas-Boas; Cordeiro-Moura, Jhonatha Rodrigo; Araujo, Maria Rita Elmor de; Gales, Ana Cristina.
Título: Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil
Fonte: Braz. j. infect. dis;21(1):57-62, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: Abstract The mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in São Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. β-lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays. The mRNA transcription level of oprD was assessed by qRT-PCR and the outer membrane proteins profile was evaluated by SDS-PAGE. Genetic relationship among P. aeruginosa isolates was assessed by PFGE. Carbapenems hydrolysis was not detected by carbapenemase assay in the carbapenem-resistant and cephalosporin-susceptible P. aueruginosa clinical isolates. OprD decreased expression was observed in all P. aeruginosa isolates by qRT-PCR. The outer membrane protein profile by SDS-PAGE suggested a change in the expression of the 46 kDa porin that could correspond to OprD porin. The isolates were clustered into 17 genotypes without predominance of a specific PFGE pattern. These results emphasize the involvement of multiple chromosomal mechanisms in carbapenem-resistance among clinical isolates of P. aeruginosa, alert for adaptation of P. aeruginosa clinical isolates under antimicrobial selective pressure and make aware of the emergence of an uncommon phenotype among P. aeruginosa clinical isolates.
Descritores: Pseudomonas aeruginosa/efeitos dos fármacos
Carbapenêmicos/farmacologia
Cefalosporinas/farmacologia
Resistência beta-Lactâmica/genética
Antibacterianos/farmacologia
-Fenótipo
Pseudomonas aeruginosa/enzimologia
Pseudomonas aeruginosa/genética
Espectrofotometria Ultravioleta
Proteínas da Membrana Bacteriana Externa
Proteínas de Bactérias/metabolismo
beta-Lactamases/metabolismo
Brasil
DNA Bacteriano
Testes de Sensibilidade Microbiana
Eletroforese em Gel de Campo Pulsado
Análise de Sequência de DNA
Porinas/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1039181
Autor: Garcia, Rita de Cássia Nasser Cubel; Pereira, Renata Freire Alves; Azevedo, Kátia Martins Lopes de; Castro, Tatiana Xavier de; Mello, Francisco C A; Setubal, Sérgio; Siqueira, Marilda M; Brown, David; Oliveira, Solange Artimos de.
Título: Molecular diversity of human parvovirus B19 during two outbreaks of erythema infectiosum in Brazil
Fonte: Braz. j. infect. dis;21(1):102-106, Jan.-Feb. 2017. graf.
Idioma: en.
Resumo: Abstract This study was conducted to provide information on the genetic diversity of human parvovirus B19 (B19V) circulating in the municipality of Niterói, Rio de Janeiro, Southeast Brazil during 1996-2006, a period with two distinct outbreaks of B19V infection: 1999-2000 and 2004-2005. A total of 27 sera from patients with erythema infectiosum and five sera from HIV-infected patients that tested positive for B19V DNA during the study period were analyzed. To genotype B19V strains, a semi-nested PCR for partial amplification of the capsid gene was performed and sequence analysis revealed that 31 sequences belonged to subgenotype 1a (G1a) of the main genotype 1 and one sequence was characterized as subgenotype 3b (G3b). The phylogenetic tree supported the division of the G1a into two well-defined clades with 1.3% of divergence. The low diversity of the G1a strains may be explained by the fact that all patients had acute B19V infection and 30/32 sera were collected during two distinct outbreaks. The G3b strain was from an HIV-infected patient who seroconverted to anti-B19 IgG antibodies in September/2005. This is the first report of G3b in the state of Rio de Janeiro.
Descritores: Surtos de Doenças
Parvovirus B19 Humano/genética
Eritema Infeccioso/epidemiologia
Eritema Infeccioso/virologia
-Filogenia
Brasil/epidemiologia
Reação em Cadeia da Polimerase
Eritema Infeccioso/genética
Análise de Sequência de DNA
Genótipo
Limites: Humanos
Masculino
Feminino
Criança
Adolescente
Adulto
Pessoa de Meia-Idade
Adulto Jovem
Responsável: BR1.1 - BIREME


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Villanova, Marcia Guimaräes
Martinelli, Ana de Lourdes Candolo
Texto completo
Id: biblio-888899
Autor: Chachá, Silvana Gama Florencio; Gomes-Gouvêa, Michele Soares; Malta, Fernanda de Mello; Ferreira, Sandro da Costa; Villanova, Márcia Guimarães; Souza, Fernanda Fernandes; Teixeira, Andreza Correa; Passos, Afonso Dinis da Costa; Pinho, João Renato Rebello; Martinelli, Ana de Lourdes Candolo.
Título: Distribution of HBV subgenotypes in Ribeirão Preto, Southeastern Brazil: a region with history of intense Italian immigration
Fonte: Braz. j. infect. dis;21(4):424-432, July-Aug. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Abstract Hepatitis B virus (HBV) is distributed worldwide, with geographical variations regarding prevalence of the different genotypes. The aim of this study was to determine the HBV genotypes and subgenotypes circulating in Southeast Brazil and compare the genetic sequences found with HBV sequences previously described in the world. Sequences from 166 chronic HBV carriers were analyzed using the fragment constituted by 1306 base pairs comprising surface and polymerase regions of the HBV genome. The sequences obtained were submitted to phylogenetic analysis. HBV subgenotypes A1, A2, D1-D4, F2a, and F4 were found. HBV genotype D was the most frequent, found in 99 patients (58.4%). Within this group, subgenotype D3 was the most prevalent, in 73 patients (42.9%). HBV genotype A was identified in 58 (36%) patients, subgenotype A1, in 48 (29.8%) subjects. Genotype F was identified in 9 (5.4%). According to the phylogenetic analysis, the sequences found were grouped with sequences from Europe, Asia and Middle East (subgenotypes D1, D2, D3) and sequences from Latin America and Africa (subgenotype A1). HBV D3 grouped in different clusters inside D3 clade, several of them with sequences isolated in Italy. We also identified eight families whose relatives were infected with the same HBV subgenotype, most with high similarity between sequences. In conclusion, the distribution of the HBV sequences obtained interweaved with sequences from other continents, corresponding to regions from where many immigrants came to this region, in accordance to the hypothesis that the HBV detected over there were brought during the colonization times.
Descritores: Vírus da Hepatite B/genética
Hepatite B Crônica/virologia
Emigrantes e Imigrantes
-Filogenia
Brasil
DNA Viral/genética
Dados de Sequência Molecular
Análise de Sequência de DNA
Emigração e Imigração
Genótipo
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME



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