Base de dados : LILACS
Pesquisa : E05.393.760.710 [Categoria DeCS]
Referências encontradas : 57 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 6 ir para página                

  1 / 57 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-838959
Autor: Zhang, Tianxiang; Peng, Dong; Qi, Lei; Li, Weixuan; Fan, Mengyuan; Shen, Jiachen; Yang, Liangliang; Wang, Yihua; Wang, Wenxia; Hu, Xiaolong; Cai, Ruibo; Zhou, Ran; Wei, Yuting; Zhou, Juntong; Yang, Shuang; Hu, Defu; Liu, Shuqiang.
Título: Musk gland seasonal development and musk secretion are regulated by the testis in muskrat (Ondatra zibethicus)
Fonte: Biol. Res;50:10, 2017. graf.
Idioma: en.
Projeto: China Postdoctoral Science Foundation; . China Postdoctoral Science Foundation; . Zhangzhou Pien Tze Huang Pharmaceutical Co., Ltd.
Resumo: BACKGROUND: The muskrat is a seasonal breeder. Males secrete musk to attract females during the breeding season. The testosterone binding to the androgen receptor (AR) in musk glands of muskrat may play an important role conducting the musk secretion process. METHODS: The musk gland, testis and blood samples of musk rats are collected in both breeding and non-breeding seasons. Some part of the samples are kept in liquid nitrogen for transcriptome analysis and Western blotting test. Some part of the samples are kept in 70% alcohol for histology experiment, blood samples are kept at -20 °C for the serum testosterone measurement experiment. RESULTS: This study demonstrates that the quantity of secreted musk, the volume of the musk glands, the diameter of the gland cells and AR expression are all higher during the breeding season than at other times (p < 0.01). StAR, P450scc and 3ß-HSD expression in the Leydig cells of the testis were also higher during this season, as was serum testosterone. AR was also observed in the gland cells of two other musk-secreting animals, the musk deer and small Indian civet, in their musk glands. These results suggest that the testes and musk glands co-develop seasonally. CONCLUSION: The musk glands' seasonal development and musk secretion are regulated by the testes, and testosterone plays an important role in the seasonal development of musk glands.
Descritores: Glândulas Odoríferas/crescimento & desenvolvimento
Glândulas Odoríferas/metabolismo
Testículo/metabolismo
Ácidos Graxos Monoinsaturados/metabolismo
-Tamanho do Órgão
Valores de Referência
Reprodução/fisiologia
Glândulas Odoríferas/anatomia & histologia
Estações do Ano
Testículo/crescimento & desenvolvimento
Testosterona/sangue
Cruzamento
Ensaio de Imunoadsorção Enzimática
Ácidos Graxos Monoinsaturados/análise
Imuno-Histoquímica
Receptores Androgênicos/análise
Receptores Androgênicos/metabolismo
Western Blotting
Arvicolinae
Análise de Sequência de RNA
Células Intersticiais do Testículo/metabolismo
Limites: Animais
Masculino
Responsável: CL1.1 - Biblioteca Central


  2 / 57 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-838964
Autor: Du, Guankui; Xiao, Man; Zhang, Xuezi; Wen, Maoyu; Pang, Chi; Jiang, Shangfei; Sang, Shenggang; Xie, Yiqiang.
Título: Alpinia oxyphylla Miq. extract changes miRNA expression profiles in db-/db- mouse kidney
Fonte: Biol. Res;50:9, 2017. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Scientific Research Fund of Hainan Education Department.
Resumo: BACKGROUND: A number of dysregulated miRNAs have been identified and are proposed to have significant roles in the pathogenesis of type 2 diabetes mellitus or renal pathology. Alpinia oxyphylla has shown significant anti-inflammatory properties and play an anti-diabetes role. The objective of this study was to detect the alteration of miRNAs underlying the anti-diabetes effects of A. oxyphylla extract (AOE) in a type II diabetic animal model (C57BIKsj db-/db-). RESULTS: Treatment with AOE for 8 weeks led to lower concentrations of blood glucose, urine albumin, and urine creatinine. 17 and 13 miRNAs were statistically identified as differentially regulated in the DB/DB and db-/db- AOE mice, respectively, compared to the untreated db-/db- mice. Of these, 7 miRNAs were identified in both comparison groups, and these 7 miRNAs were verified by quantitative real-time PCR. Functional bioinformatics showed that the putative target genes of 7 miRNAs were associated with several diabetes effects and signaling pathways. CONCLUSIONS: These founding suggest that the potential of AOE as a medicinal anti-diabetes treatment through changes in the expressions of specific miRNAs. The results provide a useful resource for future investigation of the role of AOE-regulated miRNAs in diabetes mellitus.
Descritores: Extratos Vegetais/farmacologia
MicroRNAs/efeitos dos fármacos
Diabetes Mellitus Tipo 2/tratamento farmacológico
Hipoglicemiantes/farmacologia
Rim/efeitos dos fármacos
-Fatores de Tempo
Glicemia/análise
Regulação da Expressão Gênica
Reprodutibilidade dos Testes
Resultado do Tratamento
Análise de Sequência de RNA
Creatinina/sangue
MicroRNAs/metabolismo
Diabetes Mellitus Experimental/metabolismo
Diabetes Mellitus Experimental/tratamento farmacológico
Diabetes Mellitus Tipo 2/metabolismo
Nefropatias Diabéticas/metabolismo
Nefropatias Diabéticas/tratamento farmacológico
Albuminúria
Reação em Cadeia da Polimerase em Tempo Real
Rim/metabolismo
Camundongos Endogâmicos C57BL
Limites: Animais
Masculino
Camundongos
Tipo de Publ: Estudo de Avaliação
Responsável: CL1.1 - Biblioteca Central


  3 / 57 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-838971
Autor: Thunders, Michelle; Cavanagh, Jo; Li, Yinsheng.
Título: De novo transcriptome assembly, functional annotation and differential gene expression analysis of juvenile and adult E. fetida, a model oligochaete used in ecotoxicological studies
Fonte: Biol. Res;50:7, 2017. tab, graf.
Idioma: en.
Resumo: BACKGROUND: Earthworms are sensitive to toxic chemicals present in the soil and so are useful indicator organisms for soil health. Eisenia fetida are commonly used in ecotoxicological studies; therefore the assembly of a baseline transcriptome is important for subsequent analyses exploring the impact of toxin exposure on genome wide gene expression. RESULTS: This paper reports on the de novo transcriptome assembly of E. fetida using Trinity, a freely available software tool. Trinotate was used to carry out functional annotation of the Trinity generated transcriptome file and the transdecoder generated peptide sequence file along with BLASTX, BLASTP and HMMER searches and were loaded into a Sqlite3 database. To identify differentially expressed transcripts; each of the original sequence files were aligned to the de novo assembled transcriptome using Bowtie and then RSEM was used to estimate expression values based on the alignment. EdgeR was used to calculate differential expression between the two conditions, with an FDR corrected P value cut off of 0.001, this returned six significantly differentially expressed genes. Initial BLASTX hits of these putative genes included hits with annelid ferritin and lysozyme proteins, as well as fungal NADH cytochrome b5 reductase and senescence associated proteins. At a cut off of P = 0.01 there were a further 26 differentially expressed genes. CONCLUSION: These data have been made publicly available, and to our knowledge represent the most comprehensive available transcriptome for E. fetida assembled from RNA sequencing data. This provides important groundwork for subsequent ecotoxicogenomic studies exploring the impact of the environment on global gene expression in E. fetida and other earthworm species.
Descritores: Oligoquetos/genética
Perfilação da Expressão Gênica/métodos
Ecotoxicologia
Transcriptoma
-Oligoquetos/efeitos dos fármacos
Poluentes do Solo/toxicidade
Software
Análise de Sequência de RNA/métodos
Toxicogenética/métodos
Exposição Ambiental
Ontologia Genética
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


  4 / 57 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-950822
Autor: Aftab, Usman; Zechel, David L; Sajid, Imran.
Título: Antitumor compounds from Streptomyces sp. KML-2, isolated from Khewra salt mines, Pakistan
Fonte: Biol. Res;48:1-10, 2015. ilus, graf, tab.
Idioma: en.
Resumo: BACKGROUND: Actinomycetes are gram positive bacteria with high G + C content in their DNA and are capable of producing variety of secondary metabolites. Many of these metabolites possess different biological activities and have the potential to be developed as therapeutic agents. The aim of the present study was to screen actinomycetes inhabiting halophilic environment such as Khewra salt mines present in Pakistan for cytotoxic and antitumor compounds. RESULTS: An actiomycetes strain designated as Streptomyces sp. KML-2 was isolated from a saline soil of Khewra salt mines, Pakistan. The strain Streptomyces sp. KML-2 showed 84 % cytotoxic activity against larvae of Artemiasalina. In the screening phase, the strain exhibited significant antitumor activity with IC50 values of 12, 48 and 56 µg/ml against Hela, MDBK and Vero cell lines, respectively. After that extract from 20 l fermentation was used to purify secondary metabolites by several chromatographic techniques. Structure elucidation of isolated compounds revealed that it is highly stable producer of Chromomycin SA (1) and 1-(1H-indol-3-yl)-propane-1,2,3-triol (2). Both of the isolated compounds showed significant antitumor activity against Hela and MCF-7 cancer cell lines (IC50 values 8.9 and 7.8 µg/ml against Hela; 12.6 and 0.97 µg/ml against MCF-7, respectively). The 16S rRNA gene sequence (1437 bp) of the strain confirm its identity (99 %) with Streptomyces griseus. CONCLUSIONS: From this research work we were successful in isolating two potent antitumor compounds, Chromomycin SA and 1-(1H-indol-3-yl)-propane-1,2,3-triol from Streptomyces KML-2 strain, isolated from Khewra salt mine. As such this is the second report which confirms that S. griseus can produce Chromomycin SA without introducing any mutagenesis in its biosynthesizing gene cluster and isolated indole derivative is being reported first time from any member of actinomycetes group with having novel antitumor activity against Hela and MCF-7 cells Nucleotide sequences: Nucleotide sequence data reported are available in the GenBank database under the accession number: GenBank KJ009562.
Descritores: Microbiologia do Solo
Streptomyces/química
Antineoplásicos/farmacologia
-Paquistão
Filogenia
Artemia/classificação
Artemia/efeitos dos fármacos
Sais
Solo/química
Streptomyces/isolamento & purificação
Streptomyces/ultraestrutura
Streptomyces griseus/classificação
Sais de Tetrazólio
Células Vero
RNA Ribossômico 16S/genética
Cromomicinas/classificação
Cromomicinas/farmacologia
Células HeLa
Microscopia Eletrônica de Varredura
Linhagem Celular
Chlorocebus aethiops
Cromatografia/métodos
Análise de Sequência de RNA
Concentração Inibidora 50
Células MCF-7
Formazans
Glicerol/análogos & derivados
Glicerol/farmacologia
Larva/efeitos dos fármacos
Mineração
Antibacterianos/isolamento & purificação
Antibacterianos/farmacologia
Antineoplásicos/isolamento & purificação
Limites: Humanos
Animais
Bovinos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  5 / 57 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-1100919
Autor: García, Patricia; Bizama, Carolina; Rosa, Lorena; Espinoza, Jaime A; Weber, Helga; Cerda-Infante, Javier; Sánchez, Marianela; Montecinos, Viviana P; Lorenzo-Bermejo, Justo; Boekstegers, Felix; Dávila-López, Marcela; Alfaro, Francisca; Leiva-Acevedo, Claudia; Parra, Zasha; Romero, Diego; Kato, Sumie; Leal, Pamela; Lagos, Marcela; Roa, Juan Carlos.
Título: Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor
Fonte: Biol. Res;53:13, 2020. tab, graf.
Idioma: en.
Projeto: Fondo Nacional de Desarrollo Científico y Tecnológico, FONDECYT; . Dirección de Investigación Medicina UC-Pontificia Universidad Católica de Chile; . Instituto Milenio IMII; . CONICYT.
Resumo: BACKGROUND: Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We describe here the establishment of three novel cell lines derived from the ascitic fluid of a Chilean GBC patient, who presented 46% European, 36% Mapuche, 12% Aymara and 6% African ancestry. RESULTS: After immunocytochemical staining of the primary cell culture, we isolated and comprehensively characterized three independent clones (PUC-GBC1, PUC-GBC2 and PUC-GBC3) by short tandem repeat DNA profiling and RNA sequencing as well as karyotype, doubling time, chemosensitivity, in vitro migration capability and in vivo tumorigenicity assay. Primary culture cells showed high expression of CK7, CK19, CA 19-9, MUC1 and MUC16, and negative expression of mesothelial markers. The three isolated clones displayed an epithelial phenotype and an abnormal structure and number of chromosomes. RNA sequencing confirmed the increased expression of cytokeratin and mucin genes, and also of TP53 and ERBB2 with some differences among the three cells lines, and revealed a novel exonic mutation in NF1. The PUC-GBC3 clone was the most aggressive according to histopathological features and the tumorigenic capacity in NSG mice. CONCLUSIONS: The first cell lines established from a Chilean GBC patient represent a new model for studying GBC in patients of Native American descent.
Descritores: Antígenos Glicosídicos Associados a Tumores/genética
Índios Sul-Americanos/genética
Neoplasias da Vesícula Biliar/genética
-Líquido Ascítico/metabolismo
Células Tumorais Cultivadas
Testes de Carcinogenicidade
Chile
Impressões Digitais de DNA
Proteína Supressora de Tumor p53/genética
Cisplatino/farmacologia
Camundongos Endogâmicos NOD
Células Clonais/efeitos dos fármacos
Células Clonais/metabolismo
Análise de Sequência de RNA
Receptor ErbB-2/genética
Genes erbB-2/genética
Perfilação da Expressão Gênica
Linhagem Celular Tumoral/efeitos dos fármacos
Linhagem Celular Tumoral/metabolismo
Desoxicitidina/análogos & derivados
Desoxicitidina/farmacologia
Células Epiteliais/metabolismo
Queratina-19/genética
Queratina-7/genética
Carcinogênese/genética
Neoplasias da Vesícula Biliar/metabolismo
Antineoplásicos/farmacologia
Limites: Humanos
Animais
Masculino
Pessoa de Meia-Idade
Responsável: CL1.1 - Biblioteca Central


  6 / 57 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1179202
Autor: Nakamura, Kivvi Duarte de Mello.
Título: Aspectos moleculares envolvidos no surgimento do Tumor Triplo-Negativo de Mama em pacientes portadoras ou não de mutação germinativa em BRCA1 / Molecular aspects involved in development of triple-negative breast cancer in patients with or without germline mutation in BRCA1.
Fonte: São Paulo; s.n; 2019. 140 p. ilust, tabelas, quadros.
Idioma: pt.
Tese: Apresentada a Fundação Antônio Prudente para obtenção do grau de Doutor.
Resumo: O Câncer de Mama Triplo-Negativo (CMTN), caracterizado pela perda de expressão dos receptores de estrógeno (RE), de progesterona (RP) e pela não super-expressão/amplificação do receptor do fator de crescimento epidermal humano do tipo 2 (HER-2), é considerado um subtipo bastante agressivo e heterogêneo molecularmente. Clinicamente, o CMTN apresenta prognóstico ruim, altas taxas de recorrência e menor sobrevida global em relação aos outros subtipos de câncer de mama. Ele corresponde a aproximadamente 15% dos casos de câncer de mama e não apresenta nenhuma terapia alvo efetiva. Mutação patogênica germinativa nos genes BRCA1 e BRCA2 leva a um aumento de risco para o desenvolvimento de Câncer de Mama e Ovário, sendo que mutação germinativa em BRCA1 está associada ao desenvolvimento de CMTN, especialmente em pacientes diagnosticadas antes dos 50 anos. A deficiência de BRCA1 leva ao mecanismo ineficiente do reparo do DNA e ao desenvolvimento do tumor. Recentemente, nosso grupo classificou as pacientes diagnosticadas com CMTN em grupo Hereditário (com mutação patogênica germinativa em BRCA1) e grupo Esporádico (sem mutação germinativa em BRCA1). Estes foram ainda classificados em tumores BRCA1-deficiente (com hipermetilação no promotor de BRCA1) e em tumores BRCA1-proficiente (sem hipermetilação no promotor de BRCA1 e sem mutação germinativa em BRCA1). As diferenças moleculares entre esses grupos de CMTN são de grande interesse clínico e biológico, embora ainda não sejam conhecidas. Dentro desse contexto, nosso objetivo foi investigar o perfil transcricional de CMTN sob diferentes aspectos de deficiência de BRCA1. Para isso, foi avaliado o sequenciamento do RNA (RNA-Seq) de 37 casos de CMTN em idade jovem (≤ 50 anos), sendo 9 casos Hereditário (com mutação patogênica germinativa em BRCA1) e 28 casos Esporádico. Dos casos Esporádicos, 9 foram BRCA1-deficiente (com o promotor de BRCA1 hipermetilado) e 19 BRCA1-proficiente (com o promotor de BRCA1 não hipermetilado). Utilizamos o RNA total a partir das 37 amostras tumorais e 25 amostras normais pareadas adjacentes ao tumor. As bibliotecas de cDNA foram construídas a partir do RNA total através do método de depleção do rRNA e sequenciadas na plataforma NextSeq (Illumina). Para a normalização dos dados de expressão de cada gene, foi aplicada a medida FPKM. Os critérios para a obtenção dos genes diferencialmente expressos (GDEs), nas amostras tumorais em relação às normais, foram fold change ≥ 4,0 e ≤ - 4,0 e p valor ajustado ≤ 0,05). Para classificação molecular dos tumores TN, foi utilizada a ferramenta online TNBCtype. As curvas de sobrevida global de acordo com essa classificação molecular foram calculadas através do método de Kaplan-Meier. Para verificar os processos biológicos envolvidos com a tumorigênese do CMTN, os GDEs foram submetidos a uma análise funcional in silico através do programa Ingenuity Pathway Analysis (IPA). Em média, 48.627.204 milhões de sequências foram geradas por amostra, das quais 79,5% foram mapeadas no genoma humano referência, revelando, em média, 15.071 genes expressos com pelo menos 10 sequências única mapeada por amostra. O perfil transcricional das amostras CMTN permitiu a classificação nos 7 subtipos moleculares de CMTN, sendo a maioria das amostras classificadas no subtipo imunomodulador (IM) e mesenquimal (M). No grupo das amostras BRCA1-deficiente, observamos um predomínio do subtipo IM enquanto que, nas amostras BRCA1-proficiente, houve um maior número de amostras classificadas em M. Não observamos diferença nas curvas de sobrevida global entre os dois grupos de amostras. Porém, os resultados mostraram que o subtipo IM parece ter uma tendência de melhor sobrevida global comparado com os outros subtipos, independente do status de BRCA1. A expressão diferencial das amostras tumorais em relação às amostras normais no grupo de CMTN Hereditário revelou 1965 GDEs, sendo 589 mais expressos e 1376 menos expressos no tumor; e, no grupo de CMTN Esporádico, a análise revelou 1837 GDEs, sendo 645 mais expressos e 1192 menos expressos no tumor. A partir dos GDEs de cada grupo, a análise do IPA mostrou que os dois grupos apresentaram vias canônicas enriquecidas comuns significantemente ativadas e envolvidas, de forma geral, com a Regulação do ciclo celular. Entretanto, as vias canônicas significantemente inibidas mostraram-se exclusivas em cada grupo: a via de Sinalização do receptor do glutamato foi a mais significativa (z-score = -2,12) nas amostras de CMTN Hereditário; e a via de Ativação de LXR/RXR foi a mais significativa (z-score = -2,98) nas amostras CMTN Esporádico. Além disso, observamos reguladores transcricionais significantemente ativados e inibidos exclusivamente em cada grupo. Os genes TNF e E2F1 foram os mais significantemente ativados apenas nas amostras de CMTN Hereditário (z-score = 4,06) e de CMTN Esporádico (z-score = 2,22), respectivamente. Por outro lado, o microRNA mir-21 e o gene PPARG foram os reguladores inibidos exclusivamente nas amostras CMTN Hereditário (-score = -4,68) e nas amostras CMTN Esporádico (z-score = -4,24), respectivamente. Esse estudo, de forma geral, revelou potenciais vias e genes reguladores de cascatas de sinalização envolvidos na tumorigênese do CMTN no contexto da deficiência de BRCA1, contribuindo na elucidação da complexidade funcional de tumores TN

Triple-negative breast cancer (TNBC), characterized by lack of expression of the estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2), results in aggressive biology, early peak of recurrence, and shorter overall survival than other subtypes. It comprises approximately 15% of breast cancer cases and yet there is no effective therapy. Mutations in the BRCA1 and BRCA2 genes are associated with increased risk of breast and ovarian cancers since germline mutation in BRCA1 is associated with the development of TNBC, especially in patients diagnosed before age 50. BRCA deficiency leads to impaired DNA repair and tumor development. Recently, our group classified TNBC patients into hereditary BRCA1-mutated and sporadic BRCA1-proficient. These were further classified into BRCA1-deficient tumors (with BRCA1 promotor hypermethylation) and BRCA1-proficient tumors (no BRCA1 promoter hypermethylation neither BRCA1 germline mutation). Molecular differences between hereditary and sporadic TNBC groups are clinically and biologically interesting although it remains unclear. In this context, we aimed to investigate the transcriptional profile of TNBC-associated or not with BRCA1 deficiency. For that, RNA sequencing (RNA-seq) from 37 early-onset TNBC (≤ 50 years old) was evaluated, comprising 9 Hereditary (with BRCA1 germline pathogenic mutation) and 28 Sporadic cases, of which 9 BRCA1-deficient and 19 BRCA1-proficient. Total RNA from 37 tumors samples and 25 adjacent normal samples of paired cases were used to constructed RNAseq libraries by depleting ribosomal RNA and sequenced on Illumina NextSeq 500 platform. Expression values were normalized by FPKM. Differentially expressed genes (DEGs) between tumor and normal samples were obtained using fold-change ≥ |4| and p value adjusted ≤ 0.05 as statistical criteria. The TNBC samples were subtyping using the web-based prediction tool TNBCType. Overall survival curves were calculated using the Kaplan.Meir method. IPA software was used to detect activated/inactivated canonical pathways and relevant upstream regulators, considering a z-score ≤-2.0 and ≥ 2.0, respectively. On average, 49 million reads were generated per sample, of which 79.5% were mapped to the human reference genome revealing 15,071 expressed genes with at least 10 reads per sample. From the transcriptional profile of TNBC samples we classified into seven TNBC subtypes being the majority of tumors classified as immunomodulatory (IM) and mesenchymal (M) subtype. We detected no difference in overall survival for both groups. However, trends towards better overall were observed for TNBC samples classified as IM compared with other subtypes, without associations with BRCA1 status. Differential gene expression analysis between tumor and normal samples in the hereditary group revealed 1,965 DEGs, being 589 upregulated and 1,376 downregulated; and in the sporadic group, the analysis revealed 1,837 DEGs, being 645 upregulated and 1,192 downregulated. Using the DEGs of each group, the IPA analysis revealed that Cell Cycle Regulation signaling was activated in both groups. Regarding inactivated pathways, we detected the Glutamate Receptor signaling (z-score = -2,12) in hereditary TNBC and the LXR/RXR activation in sporadic TNBC (z-score = -2,98). Also, the IPA analysis revealed relevant specific transcription regulators of each group. The TNF and E2F1 were the most significantly activated genes in hereditary- and sporadic-TNBC, respectively. On the other hand, the mir-21 and PPARG were the most significantly unique inhibited regulators in hereditary and sporadic, respectively. In general, this study unveiled potential pathways and regulatory genes for signaling cascades involved in TNBC tumorigenesis considering the deficiency of BRCA1, contributing to the elucidation of the functional complexity of the tumorigenic process of TNBC patients
Descritores: Neoplasias da Mama
Análise de Sequência de RNA
Mutação em Linhagem Germinativa
Genes BRCA1
Sequenciamento de Nucleotídeos em Larga Escala
Neoplasias de Mama Triplo Negativas/genética
Limites: Humanos
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Responsável: BR30.1 - Biblioteca
BR30.1


  7 / 57 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-950861
Autor: Marthandan, Shiva; Menzel, Uwe; Priebe, Steffen; Groth, Marco; Guthke, Reinhard; Platzer, Matthias; Hemmerich, Peter; Kaether, Christoph; Diekmann, Stephan.
Título: Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence
Fonte: Biol. Res;49:1-16, 2016. ilus, graf.
Idioma: en.
Projeto: German Ministry for Education and Research.
Resumo: BACKGROUND: Cellular senescence is induced either internally, for example by replication exhaustion and cell division, or externally, for example by irradiation. In both cases, cellular damages accumulate which, if not successfully repaired, can result in senescence induction. Recently, we determined the transcriptional changes combined with the transition into replicative senescence in primary human fibroblast strains. Here, by γ-irradiation we induced premature cellular senescence in the fibroblast cell strains (HFF and MRC-5) and determined the corresponding transcriptional changes by high-throughput RNA sequencing. RESULTS: Comparing the transcriptomes, we found a high degree of similarity in differential gene expression in replicative as well as in irradiation induced senescence for both cell strains suggesting, in each cell strain, a common cellular response to error accumulation. On the functional pathway level, "Cell cycle" was the only pathway commonly down-regulated in replicative and irradiation-induced senescence in both fibroblast strains, confirming the tight link between DNA repair and cell cycle regulation. However, "DNA repair" and "replication" pathways were down-regulated more strongly in fibroblasts undergoing replicative exhaustion. We also retrieved genes and pathways in each of the cell strains specific for irradiation induced senescence. CONCLUSION: We found the pathways associated with "DNA repair" and "replication" less stringently regulated in irradiation induced compared to replicative senescence. The strong regulation of these pathways in replicative senescence highlights the importance of replication errors for its induction.
Descritores: Senescência Celular/fisiologia
Fibroblastos/efeitos da radiação
-Fatores de Tempo
Dano ao DNA
Immunoblotting
Regulação para Baixo/efeitos da radiação
Regulação para Cima/efeitos da radiação
Células Cultivadas
Análise de Variância
Senescência Celular/efeitos da radiação
Senescência Celular/genética
beta-Galactosidase/metabolismo
Análise de Sequência de RNA
Perfilação da Expressão Gênica
Feto Abortado
Reparo do DNA/efeitos da radiação
Replicação do DNA/efeitos da radiação
Fibroblastos/fisiologia
Raios gama
Pulmão
Limites: Humanos
Masculino
Responsável: CL1.1 - Biblioteca Central


  8 / 57 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-950865
Autor: Berthet, Nicolas; Descorps-Declère, Stéphane; Nkili-Meyong, Andriniaina Andy; Nakouné, Emmanuel; Gessain, Antoine; Manuguerra, Jean-Claude; Kazanji, Mirdad.
Título: Improved assembly procedure of viral RNA genomes amplified with Phi29 polymerase from new generation sequencing data
Fonte: Biol. Res;49:1-8, 2016. ilus, graf, tab.
Idioma: en.
Projeto: Institut Pasteur. Programme Transversal de Recherche.
Resumo: BACKGROUND: New sequencing technologies have opened the way to the discovery and the characterization of pathogenic viruses in clinical samples. However, the use of these new methods can require an amplification of viral RNA prior to the sequencing. Among all the available methods, the procedure based on the use of Phi29 polymerase produces a huge amount of amplified DNA. However, its major disadvantage is to generate a large number of chimeric sequences which can affect the assembly step. The pre-process method proposed in this study strongly limits the negative impact of chimeric reads in order to obtain the full-length of viral genomes. FINDINGS: Three different assembly softwares (ABySS, Ray and SPAdes) were tested for their ability to correctly assemble the full-length of viral genomes. Although in all cases, our pre-processed method improved genome assembly, only its combination with the use of SPAdes allowed us to obtain the full-length of the viral genomes tested in one contig. CONCLUSIONS: The proposed pipeline is able to overcome drawbacks due to the generation of chimeric reads during the amplification of viral RNA which considerably improves the assembling of full-length viral genomes.
Descritores: RNA Polimerases Dirigidas por DNA/genética
RNA Viral
Genoma Viral
Análise de Sequência de RNA/métodos
Montagem de Vírus
Técnicas de Amplificação de Ácido Nucleico/métodos
-Valores de Referência
Software
República Centro-Africana
Reprodutibilidade dos Testes
Alphavirus/genética
Mengovirus/genética
Biologia Computacional
Mapeamento de Sequências Contíguas
Responsável: CL1.1 - Biblioteca Central


  9 / 57 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-1011407
Autor: Yin, Hongtao; Yu, Yan.
Título: Identification of the targets of hematoporphyrin derivative in lung adenocarcinoma using integrated network analysis
Fonte: Biol. Res;52:4, 2019. tab, graf.
Idioma: en.
Resumo: BACKGROUND: Hematoporphyrin derivative (HPD) has a sensibilization effect in lung adenocarcinoma. This study was conducted to identify the target genes of HPD in lung adenocarcinoma. METHODS: RNA sequencing was performed using the lung adenocarcinoma cell line A549 after no treatment or treatment with X-ray or X-ray + HPD. The differentially expressed genes (DEGs) were screened using Mfuzz package by noise-robust soft clustering analysis. Enrichment analysis was carried out using "BioCloud" online tool. Protein-protein interaction (PPI) network and module analyses were performed using Cytoscape software. Using WebGestalt tool and integrated transcription factor platform (ITFP), microRNA target and transcription factor (TF) target pairs were separately predicted. An integrated regulatory network was visualized with Cytoscape software. RESULTS: A total of 815 DEGs in the gene set G1 (continuously dysregulated genes along with changes in processing conditions [untreated-treated with X-ray-X-ray + treated with HPD]) and 464 DEGs in the gene set G2 (significantly dysregulated between X-ray + HPD-treated group and untreated/X-ray-treated group) were screened. The significant module identified from the PPI network for gene set G1 showed that ribosomal protein L3 (RPL3) gene could interact with heat shock protein 90 kDa alpha, class A member 1 (HSP90AA1). TFs AAA domain containing 2 (ATAD2) and protein inhibitor of activated STAT 1 (PIAS1) were separately predicted for the genes in gene set G1 and G2, respectively. In the integrated network for gene set G2, ubiquitin-specific peptidase 25 (USP25) was targeted by miR-200b, miR-200c, and miR-429. CONCLUSION: RPL3, HSP90AA1, ATAD2, and PIAS1 as well as USP25, which is targeted by miR-200b, miR-200c, and miR-429, may be the potential targets of HPD in lung adenocarcinoma.
Descritores: Derivado da Hematoporfirina/farmacologia
Redes Reguladoras de Genes/genética
Adenocarcinoma de Pulmão/genética
Neoplasias Pulmonares/genética
-Proteínas Ribossômicas/efeitos dos fármacos
Proteínas Ribossômicas/genética
Fatores de Transcrição
Análise por Conglomerados
Regulação Neoplásica da Expressão Gênica
Análise de Sequência de RNA
Proteínas de Choque Térmico HSP90/efeitos dos fármacos
Proteínas de Choque Térmico HSP90/genética
Proteínas Modificadoras Pequenas Relacionadas à Ubiquitina/efeitos dos fármacos
Proteínas Modificadoras Pequenas Relacionadas à Ubiquitina/genética
MicroRNAs/metabolismo
Linhagem Celular Tumoral
Proteínas de Ligação a DNA/efeitos dos fármacos
Proteínas de Ligação a DNA/genética
Proteínas Inibidoras de STAT Ativados/efeitos dos fármacos
Proteínas Inibidoras de STAT Ativados/genética
Citometria de Fluxo
ATPases Associadas a Diversas Atividades Celulares/efeitos dos fármacos
ATPases Associadas a Diversas Atividades Celulares/genética
Adenocarcinoma de Pulmão/tratamento farmacológico
Adenocarcinoma de Pulmão/radioterapia
Neoplasias Pulmonares/tratamento farmacológico
Neoplasias Pulmonares/radioterapia
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


  10 / 57 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-1011415
Autor: Viscarra, Tamara; Buchegger, Kurt; Jofre, Ignacio; Riquelme, Ismael; Zanella, Louise; Abanto, Michel; Parker, Alyssa C; Piccolo, Stephen R; Roa, Juan Carlos; Ili, Carmen; Brebi, Priscilla.
Título: Functional and transcriptomic characterization of carboplatin-resistant A2780 ovarian cancer cell line
Fonte: Biol. Res;52:13, 2019. graf.
Idioma: en.
Projeto: National Commission for Scientific and Technological Research (CONICYT); . National Funding for Scientific and Technologic Development of Chile (FONDECYT); . Dirección de Investigación, Universidad de La Frontera (DIUFRO).
Resumo: BACKGROUND: Ovarian cancer is a significant cancer-related cause of death in women worldwide. The most used chemotherapeutic regimen is based on carboplatin (CBDCA). However, CBDCA resistance is the main obstacle to a better prognosis. An in vitro drug-resistant cell model would help in the understanding of molecular mechanisms underlying this drug-resistance phenomenon. The aim of this study was to characterize cellular and molecular changes of induced CBDCA-resistant ovarian cancer cell line A2780. METHODS: The cell selection strategy used in this study was a dose-per-pulse method using a concentration of 100 µM for 2 h. Once 20 cycles of exposure to the drug were completed, the cell cultures showed a resistant phenotype. Then, the ovarian cancer cell line A2780 was grown with 100 µM of CBDCA (CBDCA-resistant cells) or without CBDCA (parental cells). After, a drug sensitivity assay, morphological analyses, cell death assays and a RNA-seq analysis were performed in CBDCA-resistant A2780 cells. RESULTS: Microscopy on both parental and CBDCA-resistant A2780 cells showed similar characteristics in morphology and F-actin distribution within cells. In cell-death assays, parental A2780 cells showed a significant increase in phosphatidylserine translocation and caspase-3/7 cleavage compared to CBDCA-resistant A2780 cells (P < 0.05 and P < 0.005, respectively). Cell viability in parental A2780 cells was significantly decreased compared to CBDCA-resistant A2780 cells (P < 0.0005). The RNA-seq analysis showed 156 differentially expressed genes (DEGs) associated mainly to molecular functions. CONCLUSION: CBDCA-resistant A2780 ovarian cancer cells is a reliable model of CBDCA resistance that shows several DEGs involved in molecular functions such as transmembrane activity, protein binding to cell surface receptor and catalytic activity. Also, we found that the Wnt/3-catenin and integrin signaling pathway are the main metabolic pathway dysregulated in CBDCA-resistant A2780 cells.
Descritores: Neoplasias Ovarianas/genética
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Carboplatina/farmacologia
Resistencia a Medicamentos Antineoplásicos/genética
Transcriptoma/efeitos dos fármacos
Antineoplásicos/farmacologia
-Neoplasias Ovarianas/patologia
Neoplasias Ovarianas/tratamento farmacológico
Fenótipo
Transdução de Sinais
Morte Celular/efeitos dos fármacos
Morte Celular/genética
Análise de Sequência de RNA
Linhagem Celular Tumoral
Transcriptoma/genética
Limites: Humanos
Feminino
Responsável: CL1.1 - Biblioteca Central



página 1 de 6 ir para página                
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde