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Id: biblio-1283652
Autor: Zhi, Kaining; Lebo, David B.
Título: A preformulation strategy for the selection of controlled-release components to simulate a subcutaneous implant / Una estrategia de preformulación para la selección de componentes de liberación controlada para simular un implante subcutáneo
Fonte: Bol. latinoam. Caribe plantas med. aromát;19(4):344-356, 2020. tab, ilus.
Idioma: en.
Resumo: Many chronic diseases require repetitive injections as maintenance treatment. It is therefore important to investigate a possible alternative. A simulated subcutaneous implant prototype was fabricated as a polymer matrix covered by cylinder-shape tubing having a porous membrane. Sucrose, bovine serum albumin, and gelatin were selected as matrix excipients. Eight APIs with different physiochemical properties were used to investigate the releasing mechanism. Drug release was tested through an in vitrodissolution apparatus. Drug release of eight APIs followed zero-order kinetics with a minimum 12-hour duration. Release rates also showed linear correlations with the APIs' solubilities under physiological pH. For releasing mechanism studies, different combinations of matrix and membrane were investigated in detail. A 144-hour continuous zero-order release of caffeine was achieved as the best controlled simulated prototype. The results showed that drug release of our simulated prototype was primarily achieved by drug diffusion rather than dissolution.

Muchas enfermedades crónicas requieren inyecciones repetitivas como tratamiento de mantenimiento. Por lo tanto, es importante investigar una posible alternativa. Se fabricó un prototipo de implante subcutáneo simulado a partir de una matriz de polímero cubierta por un tubo en forma de cilindro que tiene una membrana porosa. La sacarosa, la albúmina de suero bovino y la gelatina se seleccionaron como excipientes matriciales. Se utilizaron ocho APIs con diferentes propiedades fisicoquímicas para investigar el mecanismo de liberación. La liberación del fármaco se probó a través de un aparato de disolución in vitro. La liberación del fármaco de las ocho APIs siguió una cinética de orden cero con una duración mínima de 12 horas. Las tasas de liberación también mostraron correlaciones lineales con las solubilidades de las APIs a pH fisiológico. Para los estudios de mecanismos de liberación, se investigaron en detalle diferentes combinaciones de matriz y membrana. El prototipo simulado con mejor control logró una liberación continua de cafeína de orden cero durante 144 horas. Los resultados mostraron que la liberación del fármaco del prototipo simulado ocurrió principalmente mediante la difusión del fármaco en lugar de la disolución.
Descritores: Preparações Farmacêuticas/administração & dosagem
Implantes de Medicamento/metabolismo
-Técnicas In Vitro
Projetos Piloto
Simulação
Cromatografia Líquida de Alta Pressão
Tela Subcutânea
Preparações de Ação Retardada
Avaliação Pré-Clínica de Medicamentos
Liberação Controlada de Fármacos
Liofilização
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1249171
Autor: Amparo, Tatiane Roquete; Rodrigues, Ivanildes Vasconcelos; Seibert, Janaína Brandão; Almeida, Tamires Cunha; Cabral, Vivette Appolinário Rodrigues; Vieira, Paula Melo de Abreu; Brandão, Geraldo Célio; Oliveira, Mauro Lúcio Gonçalves de; Silva, Glenda Nicioli da; Santos, Orlando David Henrique dos; Vieira Filho, Sidney Augusto; Teixeira, Luiz Fernando Medeiros; Souza, Gustavo Henrique Bianco de.
Título: Antibacterial substances from leaves of Protium spruceanum (Burseraceae): in vitro and in silico evaluation
Fonte: Braz. J. Pharm. Sci. (Online);56:e18474, 2020. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa de Minas Gerais.
Resumo: Due to the increase of bacterial resistance, the search for new antibiotics is necessary and the medicinal plants represent its most important source. The aim of this study was to evaluate the antibacterial property of extract and fractions from Protium spruceanum leaves, against pathogenic bacteria. By means of diffusion and microdilution assays, the crude extract was active against the nine bacteria tested being the hydromethanolic fraction the most active. During phytochemical procedures, procyanidin (1) and catechin (2) were identified as the main antibacterial constituents of this fraction. In silico results obtained using PASSonline tool indicated 1 and 2 as having good potential to interact with different targets of currently used antibiotics. These results no indicated potential to none DNA effect and indicated the cell wall as mainly target. Electrophoresis result supported that had no DNA damage. Cell wall damage was confirmed by propidium iodide test that showed increased membrane permeability and by cell surface deformations observed in scanning electronic microscopy. The in vitro assays together with the in silico prediction results establish the potential of P. spruceanum as source of antibacterial compounds that acts on important bacterial targets. These results contribute to the development of natural substances against pathogenic bacteria and to discovery of new antibiotics.
Descritores: Plantas Medicinais/efeitos adversos
Técnicas In Vitro/métodos
Extratos Vegetais/análise
Catequina
Antibacterianos/análise
-Simulação por Computador
Microscopia Eletrônica de Varredura/métodos
Folhas de Planta/classificação
Burseraceae/classificação
Compostos Fitoquímicos
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


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Id: biblio-1285512
Autor: Nallathamby, Neeranjini; Lakshmanan, Hariprasath; Vidyadaran, Sharmili; Malek, Sri Nurestri Abdul; Phan, Chia-Wei; Sabaratnam, Vikineswary.
Título: Lipid-rich fraction of the sclerotium of Tiger Milk Mushroom Lignosus rhinocerotis (Agaricomycetes) attenuates LPS-induced inflammation in BV2 cells via Nrf2 pathway
Fonte: Braz. J. Pharm. Sci. (Online);56:e18499, 2020. tab, graf.
Idioma: en.
Projeto: University of Malaya High Impact Research; . University of Malaya.
Resumo: Lignosus rhinocerotis (tiger milk mushroom) is widely used by the indigenous people of Malaysia as a traditional remedy. The present study was carried out in order to evaluate the antioxidant, cytotoxic and anti-neuroinflammatory activities of L. rhinocerotis extract on brain microglial cells (BV2). The antioxidant activity was evaluated by 2,2-diphenyl-1-picryhydrazyl (DPPH•), 2,2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS•+) scavenging assays, and ferric reducing antioxidant power (FRAP). The FRAP, DPPH and ABTS•+ scavenging capacities of the TE3 fraction were 420.77 mg FE/g, 58.01%, and 7%, respectively. The cytotoxic activity was determined by MTS assay. The in vitro model of anti-neuroinflammatory property was evaluated by measuring the production of nitric oxide (NO) in lipopolysaccharide (LPS)-induced BV2 cells. The TE3 fraction showed a significant NO reduction at 1 to 100 µg/mL. The TE3 fraction down-regulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX2) genes while it upregulated heme oxygenase (HO-1) and NADPH quinone acceptor oxidoreductase-1 (NQO-1) genes. The nuclear factor (erythroid-derived 2)-like 2 (Nrf2) transcription was also activated. The chemical component of the active fraction (TE3) was identified by gas chromatography-mass spectrometry (GCMS). Overall, the BV2 in vitro model anti-neuroinflammatory activity of L. rhinocerotis may be caused by the lipid constituents identified in the fraction
Descritores: Técnicas In Vitro/métodos
Células/classificação
Agaricales/classificação
-Inflamação/tratamento farmacológico
Lipídeos/efeitos adversos
Cromatografia Gasosa-Espectrometria de Massas/instrumentação
Antioxidantes/farmacologia
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


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Id: biblio-1285513
Autor: Dabaghi, Nazanin; Seyfoddin, Vahid; Toliyat, Tayebeh; Ataie, Zahra.
Título: Preparation and in vitro release profiling of PLGA microspheres containing BSA as a model protein
Fonte: Braz. J. Pharm. Sci. (Online);56:e18748, 2020. tab, graf.
Idioma: en.
Projeto: Vice Chancellor for Research, International campus of Tehran University of Medical Sciences.
Resumo: Conventional drug formulations are incapable of adequate delivery of proteins and peptides for therapeutic purposes. As these molecules have very short biological half-life, multiple dosing is required to achieve the desirable therapeutic effects. Microspheres are able to encapsulate proteins and peptide in the polymeric matrix while protecting them from enzymatic degradation. In this study Bovine Serum Albumin (BSA) matrix type microspheres were fabricated using Polylactide-co-glycolide (PLGA) by double emulsion solvent evaporation method. The effects of variables such as homogenizer speed, molecular weight of polymer and the effect of pH of the water phases, were investigated against factors such as drug loading, encapsulation efficiency, morphology, size, drug distribution and release profile of the microspheres. Results, suggested that an increase in homogenization speed leads to a decrease in microsphere size. The increase in homogenization speed also caused a significant effect on the release profile only when higher molecular weight of polymer had been used.. The pH change of the internal aqueous phase led to modification of surface morphology of spheres to a porous structure that significantly increased the total amount of released protein. Integrity of protein structure was intact as shown by SDS-PAGE. According to the results, it can be concluded that we achieved a reproducible method regarding controlled protein delivery for different sizes of particles.
Descritores: Técnicas In Vitro/métodos
Preparações Farmacêuticas/análise
Proteínas
Microesferas
-Soroalbumina Bovina/administração & dosagem
Eficiência/classificação
Eletroforese em Gel de Poliacrilamida/instrumentação
Emulsões
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


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Id: biblio-1282724
Autor: Bessa, Stephany Cimarosti Figueiredo; Almeida, Jéssika Raissa Medeiros de; Medeiros, Thiago Clístines de; Amorim, Débora Michelle Gonçalves; Araújo, Diana Ferreira Gadelha de; Medeiros, Maria Cristina dos Santos; Ferreira, Isana Álvares; Caldas, Marília Regalado Galvão Rabelo.
Título: Efeito do acabamento e polimento sobre a topografia superficial de uma resina composta convencional e uma bulk fill / Effect of finishing and polishing on the surface topography of a conventional compound resin and a bulk fill / Efecto de acabado y pulido en la topografía superficial de una resina compuesta convencional y un llenado a granel
Fonte: Rev. Ciênc. Plur;7(2):47-60, maio 2021. ilus, tab.
Idioma: pt.
Resumo: Introdução:As atuaisresinas Bulk-Fill apresentamcomovantagens e propriedades: menor tempo clínico, baixa contração de polimerização, maior translucidez, entre outros. Objetivo:Avaliar a topografia superficial de restaurações com diferentes tipos de resina composta antes e após submetê-las a diferentes sistemas de acabamento e polimento. Metodologia:Confecção de100 corpos de prova, sendo 50 de cada composto resinoso, apresentando cinco grupos (n=10) sorteados de forma aleatória, de acordo com o sistema de acabamento e polimento empregado. Foram utilizadas as resinas Filtek Z350 XT e a Bulk Fill Filtek One Bulk Fill e os sistemas de acabamento e polimento: Discos de Lixa Sof-Lex Pop On Kit;Discos Diamantado Espiral Sof-Lex; Ponta Enhance e Broca Carbide Multilaminada nº 0283F, que foram comparados com superfície deixada pela Tira de Poliester (Controle Negativo). Após 7 dias de armazenamento em água destilada, os corpos de provaforam submetidos aoMicroscópio Eletrônico de Varredura.Resultados:Os corpos de provada resina Bulk Fill tiveram como melhor resultado o sistema de acabamento e polimentocom broca carbidee resultado não satisfatório com discodiamantado espiral Sof-lex, enquanto a ponta Enhance e lixa Sof-lex Pop Ontiveram resultados semelhantes. Os corpos de prova da resina Filtek Z350 XT tiveram melhor aspecto visual de lisura no grupo controle negativoe pior resultado no grupo com disco diamantado espiral Sof-lex. Conclusões:Dessa forma, o sistema que apresentou melhor resultado foi a broca Carbidena resina Bulk Fill, enquanto a Filtek Z350 XT apresentou-se melhor no grupo controle. Porém, ainda são necessáriosestudos para se chegar em um protocolo de acabamento e polimento mais eficiente (AU).

Introduction:The current Bulk-Fill resins have the following advantages and properties: less clinical time, low polymerization shrinkage, greater translucency, among others.Objective:Toevaluate the topography of restorations with different types of composite resin before and after submitting them to different finishing and polishing systems.Methodology:Manufacture of 100 specimens, 50 of each resin compound, presenting five groups (n = 10) drawn at random, according to the finishing and polishing system employed. Filtek Z350 XT resins and Bulk Fill Filtek One Bulk Fill andfinishing and polishing systems were used: Sof-Lex Pop On Kit Sanding Discs; Diamond Spiral Sof-Lex Discs; Tip Enhance and Multilaminated Carbide Drill nº 0283F, which were compared with the surface left by the Polyester Strip (Negative Control). After 7 days of storage in distilled water, the specimens were submitted to a Scanning Electron Microscope.Results:The Bulk Fill resin specimens had the best result of the finishing and polishing system with carbide drill and unsatisfactory result with Sof-lex spiral diamond disc, while the Enhance tip and Sof-lex Pop On sandpaper had similar results. The Filtek Z350 XT resin specimens had a better visual aspect of smoothness in the negative control group and a worse result in the group with Sof-lex spiral diamond wheel.Conclusions:Thus, the system that showed the best result was the Carbide drill in Bulk Fill resin, while Filtek Z350 XT was better in the control group. However, studies are still needed to arrive at a more efficient finishing and polishing protocol (AU).

Introducción:Las actuales resinas Bulk-Fill tienen las siguientes ventajas y propiedades: menor tiempo clínico, baja contracción de polimerización, mayor translucidez, entre otras.Objetivo: Evaluar la topografía de restauraciones con diferentes tipos de resina compuesta antes y después de someterlas a diferentes sistemas de acabado y pulido.Metodología: Fabricación de 100 probetas, 50 de cada resina compuesta, presentando cinco grupos (n = 10) extraídos al azar, según el sistema de acabado y pulido empleado. Se utilizaron resinas Filtek Z350 XT y Bulk Fill Filtek One Bulk Fill y sistemas de acabado y pulido: Discos de lijado Sof-Lex Pop On Kit; Discos Sof-Lex en espiral de diamante; Broca Realce de Punta y Carburo Multilaminado nº 0283F, que fueron comparadas con la superficie dejada por la Tira de Poliéster (Control Negativo). Después de 7 días de almacenamiento en agua destilada, lasmuestras se sometieron a un Las muestras de resina Bulk Fill tuvieron el mejor resultado del sistema de acabado y pulido con broca de carburo y un resultado insatisfactorio con el disco de diamante en espiral Sof-lex, mientras que la punta Enhance y el papel de lija Sof-lex Pop On tuvieron resultados similares. Las muestras de resina Filtek Z350 XT tuvieron un mejor aspecto visual de suavidad en el grupo de control negativo y un peor resultado en el grupo con disco de diamante en espiral Sof-lex.Conclusiones: Así, el sistema que mejor resultado mostró fue la broca Carbide en resina Bulk Fill, mientras que Filtek Z350 XT fue mejor en el grupo control. Sin embargo, aún se necesitan estudios para llegar a un protocolo de acabado y pulido más eficiente (AU).
Descritores: Propriedades de Superfície
Microscopia Eletrônica de Varredura/instrumentação
Resinas Compostas
Polimento Dentário/instrumentação
-Técnicas In Vitro/métodos
Brasil
Protocolos
Polimerização
Tipo de Publ: Estudo Comparativo
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


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Id: biblio-1282737
Autor: Paiva, Taynã Cavalcanti de; Marinho, Maria Eduarda Lima do Nascimento; Amorim, Débora Michelle Gonçalves; Araújo, Diana Ferreira Gadelha de; Aragão, Cícero Flávio Soares; Ferreira, Isana Álvares; Caldas, Marília Regalado Galvão Rabelo.
Título: Influência do uso da proantocianidina em um adesivo autocondicionante: avaliação da resistência de união / Influence of the use of proanthocyanidinin a self-etching adhesive: evaluation of bond strength / Influenciadel uso de proantocianidinaem um adesivo autograbante: evaluación de la fuerza de unión
Fonte: Rev. Ciênc. Plur;7(2):61-73, maio 2021. tab.
Idioma: pt.
Resumo: Introdução:Os sistemas adesivos possibilitama execução de restaurações estéticas e minimamente invasivas, sendo, portanto,objeto de pesquisas para contornar os problemas que se apresentam no procedimento restaurador.Objetivo:Avaliar in vitroa resistência de união de um sistema adesivo autocondicionante, e deste modificado com soluções extrativas de semente de uva.Metodologia:Duas soluções extrativas foram preparadas comextrato de semente de uva em pó dissolvido em acetona e etanol. A partir delas e de umadesivo,seis sistemas adesivos autocondicionantes experimentais foram preparados, diferindo quanto aosolvente utilizado eàsproporções entre adesivo puro e solução extrativa(7,5%, 15% e 30%). Setenta incisivos bovinos hígidos tiveram as raízes removidas com disco de carborundum e as faces vestibulares desgastadas comlixas d'água de granulação 120, 240, 600 e 1200 sob refrigeração até expor a dentina superficial. Os dentes foram distribuídos aleatoriamenteem sete grupos distintos: Controle; A7,5; A15; A30; E7,5; E15; e E30, contendo 10 elementos cada. A aplicação dos adesivos foi executada de acordo com as recomendações do fabricante do adesivo controle. A restauração foi realizada com uma matriz de silicone com dimensões 2mm de altura e 4mm de diâmetro e inserido o material restaurador em incremento único e fotopolimerizado por 40s. Após três meses armazenados em água destilada, os espécimes foram submetidos ao teste de resistência de união. Foi empregado ométodo estatísticoTeste Paramétrico Anova 1 Fator e pós-teste de Tamhane (p<0,05). Resultados:Os grupos A7,5, E7,5 e E30 não apresentaram diferença em relação ao grupo Controle; A15 e A30 mostraram desempenho estatisticamente semelhante entre si; e E15 não apresentou diferença estatística em relação aos outros adesivos.Conclusões:A adição de proantocianidina teve efeitos diferentes,dependendodos solventes e das concentrações utilizadas, mas sem alterar significativamente o desempenho do adesivo (AU).

Introduction:Adhesive systems make it possible to perform aestheticand minimally invasive restorations, being the subject of research to circumvent the problems that arise in the restorative procedure.Objective:Evaluate in vitrothe bond strength of a self-etching adhesive system,and modified with extractive grape seed solutions. Methodology:Two extractive solutions were prepared with powdered grape seed extract dissolved in acetone and ethanol. From them and an adhesive, six experimental self-etching adhesive systems were prepared, differing in terms of the solvent used and the proportions between pure adhesive and extractive solution(7.5%, 15% and 30%). Seventy healthy bovine incisors had their roots removed with carborundum disc and the vestibular faces were worn with sandpaper with granulation water 120, 240, 600 and 1200 under refrigeration until the superficial dentin was exposed. The teeth were randomly assigned to seven different groups: Control; A7.5; A15; A30; E7.5; E15; and E30, containing 10 elements each. The application of the adhesives was carried out according to the recommendations of the manufacturer of the control adhesive. The restoration was performed with a silicone matrix with dimensions 2mm high and 4mm indiameter and the restorative material was inserted in a single increment and light cured for 40s. After three months stored in distilled water, the specimens were submitted to the bond strength test. The statistical method Parametric Test Anova 1 Factor and Tamhane post-test (p<0.05) were used. Results:Groups A7.5, E7.5 and E30 showed no difference in relation to the Control group; A15 and A30 showed a statistically similar performance; and E15 showed no statistical difference in relation to the other adhesives. Conclusions:The addition of proanthocyanidin had different effects, depending on the solvents and concentrations used, but without significantly altering the performance ofthe adhesive (AU).

Introducción: Sistemas adhesivos permiten realizar restauraciones estéticas y mínimamente invasivas, siendo objeto de investigación para sortear problemas que surgen en elprocedimiento restaurador. Objetivo: Evaluar in vitrola fuerza de unión de un sistema adhesivoautograbante y modificado con soluciones extractivas de semilla de uva. Metodología: Se prepararon dos soluciones extractivas con extracto de semilla de uva en polvo disuelto en acetona y etanol. A partir de ellos y de un adhesivo, se prepararon seis sistemas experimentales de adhesivos autograbantes, que se diferencian en cuanto al solvente utilizado y las proporciones entre adhesivo puro y solución extractiva (7,5%, 15% y 30%). Setenta incisivos bovinos sanos fueron removidos con un disco de carborundo y las caras vestibulares fueron usadas com lija de agua de granulación 120, 240, 600 y 1200 bajo refrigeración hasta que la dentina superficial quedo expuesta. Los dientes se asignaron aleatoriamente a siete grupos diferentes: Control; A7,5; A15; A30; E7,5; E15; y E30, que contiene 10 elementos cada uno. La aplicación de los adhesivos se realizó siguiendo las recomendaciones del fabricante del adhesivo de control. La restauración se realizó con matriz de silicona con 2mm de altura y 4mm de diámetro y el material restaurador se insertó en un solo incremento y se fotopolimerizó durante 40s. Tres meses después, almacenados em agua destilada, las muestras se sometieron a la prueba de resistencia de la unión. Se utilizó el método estadístico Prueba Paramétrica Factor Anova 1 y post-prueba de Tamhane (p<0,05). Resultados: Los grupos A7,5, E7,5 y E30 no mostraron diferencias em relación con el grupo Control; A15 y A30 mostraron un desempeño estadísticamente similar; y E15 no mostró diferencia estadística en relación con los otros adhesivos. Conclusiones: La adición de proantocianidina tuvo diferentes efectos, dependiendo de los disolventes y concentraciones utilizadas, pero sin alterar significativamente el rendimiento del adhesivo (AU).
Descritores: Polimento Dentário/instrumentação
Proantocianidinas
Resistência de Materiais
Resistência à Flexão
-Solventes
Técnicas In Vitro/métodos
Brasil
Epidemiologia Experimental
Análise de Variância
Cimentos Dentários/química
Extrato de Sementes de Uva
Limites: Animais
Bovinos
Tipo de Publ: Estudo Comparativo
Estudo de Avaliação
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


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Id: biblio-975767
Autor: Vilchez-Fuentes-Rivera, Katherine; Rumiche, Francisco Aurelio; Tay, Lidia Yileng.
Título: Efecto del Extracto de Maíz Morado "Chicha Morada" durante el blanqueamiento Dental. In vitro / Effect of Purple Corn Beverage "Chicha Morada" During Dental Bleaching. In vitro
Fonte: Int. j. odontostomatol. (Print);12(4):416-422, dic. 2018. tab, graf.
Idioma: es.
Resumo: RESUMEN: El resultado del tratamiento de blanqueamiento dental puede verse afectado en pacientes que consumen bebidas pigmentantes durante el tratamiento. El objetivo de este trabajo consistió en valuar el efecto in vitro de la exposición al extracto de maíz morado (chicha morada) sobre el color del esmalte humano, durante y después del tratamiento del blanqueamiento dental con peróxido de hidrógeno al 35 %. Se utilizaron 48 dientes humanos, divididos en grupos según la bebida a la que se expuso: Extracto de maíz morado peruano (MM), té verde (T) y agua destilada (A); la mitad de los especímenes expuestos a cada bebida fueron sometidos a blanqueamiento dental con peróxido de hidrógeno al 35 % durante los primeros días de exposición a la pigmentación, resultando en los siguientes grupos: Grupo A: Sin blanqueamiento + maíz morado, Grupo B: Sin blanqueamiento + té verde, Grupo C: Sin blanqueamiento + agua destilada, Grupo D: Con blanqueamiento + maíz morado, Grupo E: Con blanqueamiento + té verde, Grupo F: Con blanqueamiento + agua destilada. Los cambios de color se midieron con un espectrofotómetro digital (VITA Easyshade Advance 4.0, VITA, Alemania) antes del blanqueamiento, durante el blanqueamiento, finalizado el blanqueamiento y al final de los 36 días de exposición a los pigmentos. Según el ∆E, el extracto de maíz morado difiere significativamente con el agua destilada (p < 0,05). Con respecto al grado de luminosidad, el extracto de maíz morado con blanqueamiento presentó los menores valores de luminosidad (p < 0,05). En cuanto al croma, no hubo diferencias entre los grupos (p > 0,05). La exposición al extracto de maíz morado pigmenta los dientes, durante el blanqueamiento el extracto de maíz morado no afecta el tratamiento, pero si la exposición continúa luego del blanqueamiento dental, el color de los dientes se verá afectado.

ABSTRACT: The result of tooth whitening treatment may be affected in patients who consume staining drinks during treatment. The aim of this work was to evaluate the in vitro effect of the exposure to purple corn extract (chicha morada) on human enamel color, during and after the treatment of teeth whitening with 35 % hydrogen peroxide. Forty-eight human teeth were used, divided into groups according to the drink to which it was exposed: Peruvian purple corn extract (MM), green tea (T) and distilled water (A); half of the specimens exposed to each drink were subjected to tooth whitening with 35 % hydrogen peroxide during the first days of exposure to pigmentation, resulting in the following groups: Group A: Without bleaching + purple corn, Group B: Without whitening + green tea, Group C: No whitening + distilled water, Group D: With whitening + purple corn, Group E: With whitening + green tea, Group F: With whitening + distilled water. The color changes were measured with a digital spectrophotometer (VITA Easyshade Advance 4.0, VITA, Germany) before bleaching, during whitening, after whitening and at the end of 36 days of exposure to pigments. According to the ∆E, the purple corn extract differs significantly with the distilled water (p <0.05). Regarding the degree of luminosity, the purple corn extract with whitening presented the lowest luminosity values (p <0.05). As for the chroma, there were no differences between the groups (p> 0.05). Exposure to purple corn extract pigments the teeth, during whitening the purple corn extract does not affect the treatment, but if the exposure continues after tooth whitening, the color of the teeth will be affected.
Descritores: Pigmentos Biológicos/química
Clareamento Dental
Clareadores Dentários
-Peru
Bebidas
Técnicas In Vitro
Espectrofotômetros
Comissão de Ética
Zea mays/classificação
Zea mays/química
Sensibilidade da Dentina
Peróxido de Hidrogênio
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


  8 / 5849 LILACS  
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Texto completo SciELO Brasil
Silva, Edson Luiz da
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Id: biblio-1285555
Autor: Pagliosa, Cristiane Manfé; Vieira, Francilene Gracieli Kunradi; Silveira, Taís Thomsen; Krieguer, Jéssica Ramos; Medeiros, Marcela Freitas; Silva, Edson Luiz da.
Título: Elevated Iron Chelating Activity of Ilex paraguariensis Leaf Infusion: in vitro and in vivo Pilot Studies
Fonte: Braz. arch. biol. technol;64:e21200205, 2021. tab, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: Abstract This study presents an Ilex paraguariensis leaf infusion with important potential as natural iron-chelating. The impact of infusion time and the water volume to obtain an Ilex paraguariensis leaf infusion with high phenolic content and iron chelating activity, such as the stability of these proprieties in the storage time and temperature (immediately and after 24 h at 8 and 25 (C) were assessed. The acute consumption effect of this infusion to reduce iron absorption in vivo was also evaluated. A preliminary crossover trial with volunteers that ingested a meal containing non-haem iron (11.4 mg) with the treatments: Ilex paraguariensis leaf infusion with the highest phenolic content and iron chelating activity (200 mL) or control (200 mL water). Blood samples were withdrawn before and 1, 2, 3 and 4 h after the meal for serum iron measurement. The highest phenolic content (18.1 mg/mL) and iron chelating activity ((100%) were observed for 10 min infusion time using 30 g leaves/300 mL water. Storage at 8 or 25 (C for 24 h decreased total phenolics and di-caffeoylquinic acids by 23.5% and 25.5%, respectively (p< 0.05), without affecting the iron-chelating activity due to a saturating chelating effect at 3.34 mg/mL phenolic content. Inhibition of the iron absorption in vivo by infusion was 78% considering the iron recovery at peak maximum. The in vitro and preliminary in vivo results showed a functional property of the Ilex paraguariensis leaf infusion that may be useful for adjuvant management of iron overload diseases.
Descritores: Terapia por Quelação
Quelantes de Ferro/uso terapêutico
Ilex paraguariensis/efeitos adversos
Compostos Fenólicos
-Técnicas In Vitro
Responsável: BR1.1 - BIREME


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Id: biblio-990060
Autor: Merizalde, Gabriel; Lopera, Daniela; Villegas, Mauricio; Restrepo, Luz Marina.
Título: Leucocyte Platelet Rich Fibrin with Autologous Gingival Fibroblasts in the Treatment of Adjacent Recession Defects / Fibrina Rica en Plaquetas Leucocitarias con Fibroblastos Gingivales Autólogos en el Tratamiento de Defectos de Recesión Adyacentes
Fonte: Int. j. odontostomatol. (Print);13(1):23-30, mar. 2019. tab, graf.
Idioma: en.
Resumo: ABSTRACT: Periodontium can submit changes that lead to loss of integrity, such as periodontal disease, immune disorders or traumatic brushing. One of the most common consequences resulting from these events is the apical migration of gingival marginal tissue. Among biomaterials used for periodontal tissue regeneration, fibrin matrices have received significant attention to correct gingival recessions. Five oral mucosa biopsies were extracted, fibroblasts were in vitro cultured and frozen in liquid nitrogen. Three 10 mL glass sterile tubes were filled with patient blood and centrifuged immediately; clots were extracted and compressed to obtain L-PRF membranes. Autologous oral mucosa fibroblasts were added to the membranes and surgical procedures were performed in five patients. L-PRF fibrin network pore size was too small to allow human fibroblasts penetration but they were firmly attached to membrane surface. Gingival fibroblasts from fresh cell culture and recently thawed were used to attach on the L-PRF membranes. It was possible to establish a protocol for blood collection, centrifugation, fibrin clot compression, fibroblast adhesion to the membrane surface and patient application in a relatively short time (1 hour-1 hour and 30 minutes). Two patients expressed pain symptoms and the other ones presented light swelling without pain. In the first week, adjacent tissue showed few inflammation signs. Research efforts are being conducted to develop more conservative surgical techniques and new biomaterials that can promote cellular proliferation. Because of its properties, L-PRF membranes represent a tempting alternative. A combined technique to treat adjacent recession defects with L-PRF membranes and autologous oral mucosa fibroblasts in a coronal displaced flap did not show initial advantage compared with a gold standard surgery that includes an autologous soft tissue graft. Nevertheless, it could be an alternative for clinical application as a new functional cell biomaterial. More clinical evidence is needed.

RESUMEN: El periodonto puede presentar cambios que conducen a la pérdida de integridad, como la enfermedad periodontal, los trastornos inmunes o el cepillado traumático. Una de las consecuencias más comunes que resultan de estos eventos es la migración apical del tejido marginal gingival. Entre los biomateriales utilizados para la regeneración del tejido periodontal, las matrices de fibrina han recibido una atención significativa para corregir las recesiones gingivales. Se extrajeron cinco biopsias de mucosa oral, los fibroblastos se cultivaron in vitro y se congelaron en nitrógeno líquido. Tres tubos de vidrio estériles de 10 ml se llenaron con sangre del paciente y se centrifugaron inmediatamente. Los coágulos fueron extraídos y comprimidos para obtener membranas de L-PRF. Se agregaron fibroblastos autólogos de mucosa oral a las membranas y se realizaron procedimientos quirúrgicos en cinco pacientes. El tamaño de poro de la red de fibrina L-PRF era demasiado pequeño para permitir la penetración de los fibroblastos humanos, pero estaban firmemente unidos a la superficie de la membrana. Se usaron fibroblastos gingivales de cultivos de células frescas y recientemente descongelados para unirlos a las membranas L-PRF. Fue posible establecer un protocolo para la extracción de sangre, centrifugación, compresión de coágulos de fibrina, adhesión de fibroblastos a la superficie de la membrana y aplicación al paciente en un tiempo relativamente corto (1 hora, 1 hora y 30 minutos). Dos pacientes expresaron síntomas de dolor y los otros presentaron hinchazón leve sin dolor. En la primera semana, el tejido adyacente mostró pocos signos de inflamación. Se están realizando esfuerzos de investigación para desarrollar técnicas quirúrgicas más conservadoras y nuevos biomateriales que puedan promover la proliferación celular. Debido a sus propiedades, las membranas L-PRF representan una alternativa tentadora. Una técnica combinada para tratar los defectos de recesión adyacentes con membranas de L-PRF y fibroblastos de mucosa oral autóloga en un colgajo coronal desplazado no mostró una ventaja inicial en comparación con una cirugía estándar que incluye un injerto de tejido blando autólogo. Sin embargo, podría ser una alternativa para la aplicación clínica como un nuevo biomaterial de células funcionales. Se necesita más evidencia clínica.
Descritores: Estomatite
Lipopolissacarídeos/farmacologia
Citocinas/efeitos dos fármacos
Porphyromonas gingivalis/metabolismo
Fibroblastos/efeitos dos fármacos
Fibrina Rica em Plaquetas
Retração Gengival/cirurgia
-Cicatrização/fisiologia
Biópsia
Técnicas In Vitro
Periodonto
Microscopia Eletrônica
Comissão de Ética
Colômbia
Gengiva/citologia
Consentimento Livre e Esclarecido
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1045999
Autor: Ma, Sen; Zhou, Guangxian; Chen, Yulin.
Título: Effects of all-trans retinoic acid on goat dermal papilla cells cultured in vitro
Fonte: Electron. j. biotechnol;34:43-50, july. 2018. tab, graf, ilus.
Idioma: en.
Projeto: National Natural Science Foundation of China; . China Agriculture Research System.
Resumo: Background: All-trans retinoic acid (ATRA), a vitamin A-derived active metabolite, exerts important functions in hair biology. Previous studies indicated that excess ATRA hampered hair follicle morphogenesis and cyclic regeneration in adulthood, but other studies stated that ATRA promoted hair growth. Dermal papilla (DP), a cluster of specialized fibroblasts, plays pivotal roles in controlling development and regeneration of hair follicle. Several lines of evidence indicated that DP might be the target cells of ATRA in the hair follicle. To confirm this hypothesis, the present study was performed to explore the biological effects of ATRA on goat dermal papilla cells (DPCs) and clarify the roles of ATRA in hair biology. Results: Our experimental results indicated that key signaling transducers of ATRA were dynamically expressed in distinct stages of goat cashmere growth cycle, and high-dose ATRA treatment (10-5 M) significantly impaired the viability of goat DPCs and lowered the ratio of proliferating cells. Otherwise, goat DPCs were stimulated to enter apoptosis and their cell cycle progression was severely blocked by ATRA. Moreover, the expression of fibroblast growth factor 7 (Fgf7), one of the potent hair growth stimulators secreted by DPCs, was transcriptionally repressed following ATRA treatment. Conclusion: DPCs are the targets of ATRA in the hair follicle, and ATRA negatively regulates hair growth by the targeted suppression of cell viability and growth factor expression of goat DPCs. Through these observations, we offer a new mechanistic insight into the roles of ATRA in hair biology.
Descritores: Tretinoína/farmacologia
Cabras
Folículo Piloso/efeitos dos fármacos
-Regeneração
Técnicas In Vitro
Imuno-Histoquímica
Receptores do Ácido Retinoico
Folículo Piloso/citologia
Folículo Piloso/crescimento & desenvolvimento
Proliferação de Células/efeitos dos fármacos
Fator 7 de Crescimento de Fibroblastos/genética
Reação em Cadeia da Polimerase em Tempo Real
Limites: Animais
Responsável: CL1.1 - Biblioteca Central



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