Base de dados : LILACS
Pesquisa : E05.588.570 [Categoria DeCS]
Referências encontradas : 63 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 7 ir para página                  

  1 / 63 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: lil-669284
Autor: MONTOYA VILLEGAS, JULIO CÉSAR; PEÑA GONZÁLEZ, ÁNGELA; SATIZÁBAL SOTO, JOSÉ MARÍA; GARCÍAVALLEJO, FELIPE.
Título: Análisis sistémcco in silico de la expresión dierencial de genes localizados een la región crítica del síndrome de down (dscr) en el cerebro humano / In silico sytemiic analysis of the differential expression of genes localized in the down syndrome critical region (dscr) in normal human brain / Análise sistêicaa in silico da expressão difeencial de genes localizados na região crítica da síndrome de down (dscr) no cérebrohuumano
Fonte: Rev. MED;20(1):15-26, ene.-jun. 2012. ilus, tab.
Idioma: es.
Resumo: Uno de los retos más importantes de este siglo en la neurología genómica es construir mapas de expresión espacial de genes a lo largo de las distintas estructuras cerebrales con el fin de correlacionarlos con ciertas neuropatologías. Se analizaron los perfiles de transcripción de ocho genes HAS21 localizados en la región crítica del síndrome de Down en diferentes estructuras del cerebro humano normal. Se tomaron como referencia los valores de expresión de ocho genes HAS21/DSCR provenientes de experimentos de micromatrices de ADN de cerebros humanos normales y cuyos valores están disponibles en la base de datos del proyecto cerebro humano del Atlas del Cerebro del Allen Institute for Brain Sciences en Seattle, Washington (http://www.brainmap.org). Se determinó una expresión diferencial de estos genes HAS21/DSCR a lo largo de las estructuras localizadas en el lóbulo frontal, el lóbulo límbico y en los núcleos centrales. En el putamen, el núcleo caudado, el giro parahipocampal y en las áreas centrales se registraron los mayores niveles de transcripción global; estas áreas del cerebro parecen estar asociadas con diversos procesos de aprendizaje y de memoria. Se correlacionó la transcripción diferencial de genes DSCR con la localización cerebral y su potencial papel funcional.

One of the most important challenges of the 21st Century Neurology is to build gene expression profiles along the different structures of human brain trying to correlate them with some neuropathologies. The expression profiles of eight HAS21 genes located on the Down syndrome critical region in different structures of the normal human brain was analyzed. From DNA microarray experiments of normal human brains which are available in the free access human brain database of the Brain Atlas project of the Allen Institute for Brain Sciences in Seattle, Washington (http://www.brainmap.org) expression levels data of eight HSA21/DSCR genes along different structures of normal human brain were statistically analyzed. A differential expression of these genes HSA21/DSCR in some anatomic structures located in the frontal lobe, limbic lobe and cerebral central nuclei was registered. Putamen, caudate nucleus, parahipocampal gyro and central areas, showed high levels of transcription for those HSA21/DSCR genes included in the study; these areas of the brain appear to be associated with some processes of learning and memory. This study allowed us to correlate the differential transcription of DSCR genes, their structural localization and functional role in brain function.

Um dos maiores desafio deste século na neurologia genômica é construir mapas de expressão espacial de genes ao longo das diferentes estruturas cerebrais com o fim de correlacionálos com certas neuropatologias. Foram analisados os perfis de transcrição de oito genes HAS21 localizados na região crítica da síndrome de Down em diferentes estruturas do cérebro humano normal. Foram usados como referência os valores de expressão de oito genes HAS21/DSCR provenientes de experimentos de micromatrizes de ADN de cérebros humanos normais e cujos valores estão disponíveis no bando de dados do projeto cérebro humano do Atlas do Cérebro do Allen Institute for Brain Sciences em Seattle, Washington (http://www.brainmap.org). Determinouse uma expressão diferencial destes genes HAS21/DSCR ao longo das estruturas localizadas no lóbulo frontal, o lóbulo límbico e nos núcleos centrais. No putâmen, o núcleo caudado, o giro parahipocampal e nas áreas centrais foram registrados os maiores níveis de transcrição global; estas áreas do cérebro parecem estar associadas com diversos processos de aprendizagem e de memória. Correlacionouse a transcrição diferencial de genes DSCR com a localização cerebral e seu potencial papel funcional.
Descritores: Análise em Microsséries
-Síndrome de Down
Biologia Computacional
Perfilação da Expressão Gênica
Cérebro
Limites: Humanos
Tipo de Publ: Artigo Clássico
Responsável: CO87.1 - Biblioteca Médica


  2 / 63 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-902558
Autor: Faundes, Víctor; Santa María, Lorena; Morales, Paulina; Curotto, Bianca; Alliende, María Angélica.
Título: Microarreglos cromosómicos en 236 pacientes chilenos con trastornos del neurodesarrollo y anomalías congénitas / Microarrays in 236 patients with neurodevelopmental disorders and congenital abnormalities
Fonte: Rev. méd. Chile;145(7):854-861, jul. 2017. tab, graf.
Idioma: es.
Resumo: Background: In 20% of neurodevelopmental disorders (NDD) and congenital abnormalities (CA) the cause would be a genomic imbalance detectable only by chromosomal microarrays (CMA). Aim: To analyze the results of CMA performed at the INTA Laboratory of Molecular Cytogenetics, during a period of four years in patients with NDD or CA. Material and Methods: Retrospective study that included all CMA reports of Chilean patients. Age, sex, clinical diagnosis and origin were analyzed, as well as the characteristics of the finding. The percentage of cases diagnosed by CMA was calculated considering all patients with pathogenic (PV) or probably pathogenic variants (VLP). Finally, we studied the association between patients' characteristics and a positive CMA outcome. Results: A total of 236 reports were analyzed. The median age was 5.41 (range 2.25-9.33) years, and 59% were men. Ninety chromosomal imbalances were found, which corresponded mainly to deletions (53.3%), with a median size of 1.662 (range 0.553-6.673) Megabases. The diagnostic rate of CMA in Chilean patients from all over the country was 19.2%. There was a close relationship between the patient's sex and the detection of VLP/VP (p = 0.034). Conclusions: Our diagnostic rate and the association between female sex and a higher percentage of diagnosed cases are concordant with other international studies. Therefore, CMA is a valid diagnostic tool in the Chilean population.
Descritores: Anormalidades Congênitas/diagnóstico
Anormalidades Congênitas/genética
Análise em Microsséries/métodos
Transtornos do Neurodesenvolvimento/diagnóstico
Transtornos do Neurodesenvolvimento/genética
-Chile
Estudos Retrospectivos
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Criança
Responsável: CL1.1 - Biblioteca Central


  3 / 63 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1098108
Autor: Cao, Chengsong; Liu, Yong; Wang, Qun; Zhao, Jing; Shi, Ming; Zheng, Junnian.
Título: Expression of CHPF modulates cell proliferation and invasion in lung cancer
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;53(5):e9021, 2020. graf.
Idioma: en.
Projeto: This work was supported by Jiangsu Young Medical Talent; . Xuzhou Science and Technology Bureau Applied Basic Research Program.
Resumo: Lung cancer is the most common malignancy worldwide and is characterized by rapid progression, aggressive behavior, frequent recurrence, and poor prognosis. The TCGA database indicates that chondroitin polymerizing factor (CHPF) is overexpressed in human lung cancer tissues compared with normal tissues and this overexpression corresponds to shorter overall survival in lung cancer patients. In this study, to investigate the function of CHPF in lung cancer, lentiviral vectors expressing CHPF shRNA were stably transduced into A549 and H1299 cells. Compared to shCtrl cells, CHPF knockdown cells had significantly reduced proliferation. Furthermore, the silencing of CHPF in A549 and H1299 cells resulted in apoptotic induction, which led to decreased colony formation. Wound healing and transwell invasion assays revealed that CHPF could positively regulate the migration of lung cancer cells. The tumorigenic role of CHPF was also validated in nude mouse xenograft models. Affymetrix gene chip analysis indicated that CHPF regulated the proliferation and invasion of lung cancer cells through CDH1, RRM2, MKI67, and TNFRSF10B. We thus highlight CHPF as a novel target for lung cancer treatment.
Descritores: Regulação Neoplásica da Expressão Gênica
N-Acetilgalactosaminiltransferases/metabolismo
Neoplasias Pulmonares/metabolismo
-Western Blotting
N-Acetilgalactosaminiltransferases/genética
Linhagem Celular Tumoral
Análise em Microsséries
Proliferação de Células
Reação em Cadeia da Polimerase em Tempo Real
Neoplasias Pulmonares/genética
Camundongos Endogâmicos BALB C
Limites: Humanos
Animais
Feminino
Coelhos
Responsável: BR1.1 - BIREME


  4 / 63 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1153522
Autor: Wang, Qian; Huang, Jie; Chen, Xia; Wang, Jian; Fang, Fang.
Título: Transcriptomic markers in pediatric septic shock prognosis: an integrative analysis of gene expression profiles
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;54(3):e10152, 2021. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation; . Jiangsu Provincial Medical Youth Talent; . Natural Science Foundation of Jiangsu Province; . Suzhou Science and Technology project; . National Natural Science Foundation; . Major International (Regional) Joint Research Project.
Resumo: The goal of this study was to identify potential transcriptomic markers in pediatric septic shock prognosis by an integrative analysis of multiple public microarray datasets. Using the R software and bioconductor packages, we performed a statistical analysis to identify differentially expressed (DE) genes in pediatric septic shock non-survivors, and further performed functional interpretation (enrichment analysis and co-expression network construction) and classification quality evaluation of the DE genes identified. Four microarray datasets (3 training datasets and 1 testing dataset, 252 pediatric patients with septic shock in total) were collected for the integrative analysis. A total of 32 DE genes (18 upregulated genes; 14 downregulated genes) were identified in pediatric septic shock non-survivors. Enrichment analysis revealed that those DE genes were strongly associated with acute inflammatory response to antigenic stimulus, response to yeast, and defense response to bacterium. A support vector machine classifier (non-survivors vs survivors) was also trained based on DE genes. In conclusion, the DE genes identified in this study are suggested as candidate transcriptomic markers for pediatric septic shock prognosis and provide novel insights into the progression of pediatric septic shock.
Descritores: Choque Séptico/diagnóstico
Choque Séptico/genética
Transcriptoma
-Biomarcadores
Biologia Computacional
Perfilação da Expressão Gênica
Análise em Microsséries
Limites: Humanos
Criança
Responsável: BR1.1 - BIREME


  5 / 63 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1045734
Autor: Liu, J; Wang, WT; Liu, RM; Zhang, SX; Wang, XB; Gong, L; Sun, J; Duan, LJ; Sun, CM.
Título: The application of chromosome abnormality chip detection in male infertility / Aplicación de la detección de la anormalidad del cromosoma mediante biochips genéticos en la infertilidad masculina
Fonte: West Indian med. j;62(8):692-697, Nov. 2013. ilus, tab.
Idioma: en.
Resumo: OBJECTIVE: To discuss the application of microarray technology in the diagnosis of male infertility. METHODS: Sixteen loci, including a sex-determining region on the Y chromosome, were investigated by polymerase chain reaction (PCR) in infertile male patients. Chromosome abnormality chip with 180 000 probes was used to detect small deletion, small amplification and loss of heterozygosity. RESULTS: By PCR, nine of 103 infertile patients were found to have sequence-tagged site microdeletions. Microdeletions were not observed in control samples. The deletions detected by PCR were present in six azoospermic men (6/44, 13.6%) and in three oligoasthenoteratozoospermic (OATS) men (3/59, 5%). The overall frequency of microdeletions in infertile men was 8.7% (9/103). Chromosome abnormality chip detection 500+ detected more amplification or deletion in 51 infertile patients and the overall frequency of microdeletions in infertile men was 49.5% (51/103). CONCLUSION: Chromosome abnormality chip detection system provides a sensitive, economic and high-throughput method for detecting the deletion or amplification of genomic DNA sequences of infertile patients. Not only can it identify Yq deletions, but it can also find other chromosome abnormalities and facilitate the understanding of male infertility.

OBJETIVO: Analizar la aplicación de la tecnología de los microarreglos en el diagnóstico de la infertilidad masculina. MÉTODOS: Dieciséis loci, incluyendo una región determinante del sexo en el cromosoma Y, fueron investigados mediante reacción en cadena de la polimerasa (RCP) en pacientes hombres con problemas de infertilidad. Un biochip de la anormalidad cromosómica, con 180000 sondas, fue utilizado a fin de detectar pequeñas delecciones, pequeñas amplificaciones y pérdidas de heterocigosidad. RESULTADOS: Por medio de la RCP, se halló que nueve de 103 pacientes con infertilidad presentaban microdelecciones de sitios de secuencia marcada. Las microdelecciones no fueron observadas en las muestras de control. Las delecciones detectadas mediante RCP, estuvieron presentes en seis hombres azoospérmicos (6/44, 13.6%) y en tres hombres con oligoastenoteratozoospermia (OAT) (3/59, 5%). La frecuencia general de las microdelecciones en los hombres infértiles fue 8.7% (9/103). La detección con biochip de la anormalidad cromosómica de 500+ detectó más amplificación y delección en 51 pacientes, y la frecuencia general de microdelecciones en los hombres infértiles fue 49.5% (51/103). CONCLUSIÓN: El sistema de detección de la anormalidad del cromosoma mediante biochips genéticos representa un método sensible, económico, y de alto rendimiento, para detectar la delección o amplificación de las secuencias genómicas de ADN de pacientes infértiles. Este método puede no sólo identificar las delecciones Yq, sino también hallar otras anormalidades cromosómicas, facilitando así la comprensión de la infertilidad en los hombres.
Descritores: Aberrações Cromossômicas
Análise em Microsséries/métodos
Infertilidade Masculina/diagnóstico
-Reação em Cadeia da Polimerase
Limites: Humanos
Masculino
Responsável: BR1.1 - BIREME


  6 / 63 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-916682
Autor: Villa, L.
Título: VALOR AGREGADO DEL MICROARRAY CON ALÉRGENOS EN PACIENTES POLISENSIBILIZADOS CON ALERGIA RESPIRATORIA / The additional values of microarray allergen assay in the management of polysensitized patients with respiratory allergy
Fonte: Arch. alerg. inmunol. clin;45(1):37-38, 2014.
Idioma: es.
Descritores: Alérgenos
Análise em Microsséries/métodos
Hipersensibilidade/epidemiologia
-Imunização
Diagnóstico
Limites: Humanos
Criança
Tipo de Publ: Comentário
Responsável: AR144.1 - CIBCHACO - Centro de Información Biomedica del Chaco


  7 / 63 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: lil-676132
Autor: García-Garza, Rubén; Rodríguez-Vidales, Edgar Paolo; Soto-Domínguez, Adolfo.
Título: Quick and inexpensive method to elaborate tissue punches useful in paraffin tissue microarrays / Método rápido y económico para la elaboración de dispositivos de punch útiles en microarreglos de tejidos en parafina
Fonte: Int. j. morphol;31(1):50-54, mar. 2013. ilus.
Idioma: en.
Resumo: The tissue microarrays (TMAs) were first called multitumor block. In 1998 was described the current technique, that uses an innovated sampling method for more than 1,000 cylindrical paraffin tissue core biopsies in a single paraffin block. TMAs are now considered as a useful powerful research tool in Histology and Pathology laboratories, for the standardization of immunohistochemical techniques along with in situ hybridization. However, one disadvantage to its widespread use is the high cost of professional paraffin tissue punches, and the complexity in the development of homemade devices previously described in other studies. This study describes a step by step process to develop four different home-made devices made with materials that are common in hospitals and offices. These devices are useful in Histopathology laboratories to obtain paraffin blocks with until 360 samples of tissue, investing from two to fifteen dollars in the development of each device described.

Los microarreglos de tejido (TMAs) fueron llamados por primera vez como bloque multitumor. En 1998 se describió la técnica actual, que utiliza un novedoso método de muestreo para obtener más de 1,000 cilindros de biopsias de tejidos incluidos en un solo bloque de parafina. Actualmente, los TMAs se consideran una poderosa herramienta de investigación en laboratorios de Histología y Patología, para la estandarización de técnicas inmunohistoquímica e hibridación in situ entre otras. Sin embargo, uno de los inconvenientes para su uso generalizado es el alto costo de los dispositivos profesionales para tejidos en parafina, y la complejidad en la elaboración de los dispositivos caseros descritos previamente en otros estudios. Este estudio describe paso a paso el proceso de elaboración de cuatro dispositivos caseros útiles para la obtención de matrices de tejido elaborados con materiales que son comunes en hospitales y oficinas. Estos dispositivos son útiles en laboratorios de Histopatología con el fin de obtener bloques de parafina de hasta 360 muestras de tejido, con una inversión de 2 a 15 dólares en la elaboración de cada uno de los dispositivos descritos.
Descritores: Análise em Microsséries/métodos
-Parafina
Imuno-Histoquímica/métodos
Hibridização In Situ/métodos
Custos e Análise de Custo
Análise em Microsséries/economia
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


  8 / 63 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-950786
Autor: Ortiz-Severín, Javiera; Varas, Macarena; Bravo-Toncio, Catalina; Guiliani, Nicolás; Chávez, Francisco P.
Título: Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis
Fonte: Biol. Res;48:1-6, 2015. ilus, tab.
Idioma: en.
Projeto: Fondecyt.
Resumo: BACKGROUND: Pseudomonas aeruginosa is known to be a multidrug resistant opportunistic pathogen. Particularly, P. aeruginosa PAO1 polyphosphate kinase mutant (ppk1) is deficient in motility, quorum sensing, biofilm formation and virulence. FINDINGS: By using Phenotypic Microarrays (PM) we analyzed near 2000 phenotypes of P. aeruginosa PAO1 polyP kinase mutants (ppk1 and ppk2). We found that both ppk mutants shared most of the phenotypic changes and interestingly many of them related to susceptibility toward numerous and different type of antibiotics such as Ciprofloxacin, Chloramphenicol and Rifampicin. CONCLUSIONS: Combining the fact that ppk1 mutants have reduced virulence and are more susceptible to antibiotics, polyP synthesis and particularly PPK1, is a good target for the design of molecules with anti-virulence and anti-persistence properties.
Descritores: Pseudomonas aeruginosa/enzimologia
Fosfotransferases (Aceptor do Grupo Fosfato)/genética
Farmacorresistência Bacteriana Múltipla/genética
Análise em Microsséries/métodos
Mutação
-Fenótipo
Polifosfatos/metabolismo
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/patogenicidade
Rifampina/farmacologia
Virulência/genética
Ciprofloxacina/farmacologia
Cloranfenicol/farmacologia
Antibacterianos/farmacologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  9 / 63 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-1131880
Autor: Sun, Ying; Wang, Wei; Tang, Yuxiao; Wang, Daping; Li, Liang; Na, Min; Jiang, Guantong; Li, Qian; Chen, Shulin; Zhou, Jin.
Título: Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves disease
Fonte: Biol. Res;53:32, 2020. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . China Scholarship Council.
Resumo: BACKGROUND: Circulating RNA (circRNA) regulates various bioactivities in cells. A better understanding of the exosomal circRNA can provide novel insights into the pathogenesis and treatment of Graves' disease (GD). We aimed to profile the differentially expressed circRNAs (DEcRs) in plasma exosomes of patients with GD and speculate and probe the functions of the DEcR by comprehensive bioinformatics analyses. METHODS: Serum exosomes were isolated from five primary GD patients and five healthy controls via ultracentrifugation. After verification with transmission electron microscopy, exosome samples were subjected to microarray profiling using human circRNA microarrays. Two up-regulated and two down-regulated DEcRs were selected for validation in plasma exosomes from 20 GD and 20 healthy control participants using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). The circRNA/microRNA/mRNA interaction network was then assembled and the analysis of the Gene Ontology and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways was utilized to predict the potential functions of the DEcR associated genes. RESULTS: There were 15 DEcRs revealed in primary GD cases. The intronic circRNA hsa_circRNA_000102 was confirmed as an up-regulated component in plasma exosomes from patients with GD. The circRNA/microRNA/mRNA interaction network unveiled the most potential targeting microRNAs of hsa_circRNA_000102 and its associated genes. The functional analyses predicted involvement of hsa_circRNA_000102 associated genes in pathways of immune system activation, such as viral infection and interferon-beta signaling. CONCLUSIONS: hsa_circRNA_000102 is a differentially up-regulated plasma exosomal circRNA in patients with GD. Our study highlights multiple pathways, particularly virus infection and interferon-beta signaling, for mediating immune activation in Graves' disease.
Descritores: Doença de Graves/genética
Doença de Graves/sangue
Análise em Microsséries
RNA Circular/sangue
-RNA Mensageiro
MicroRNAs
Exossomos
Limites: Humanos
Masculino
Feminino
Responsável: CL1.1 - Biblioteca Central


  10 / 63 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-870254
Autor: Pinilla-Fernández, Mabel Gigliola.
Título: Avaliação da expressão de genes codificadores e não codificadores em células epiteliais do carcinoma ductal invasivo de mama de pacientes com e sem linfonodo acometido / Evaluating the expression of coding and non-coding genes in epithelial cells of invasive ductal breast cancer in patients with and without lymph node involved.
Fonte: São Paulo; s.n; 2015. 98 p. ilus, tab.
Idioma: pt.
Tese: Apresentada a Fundação Antônio Prudente para obtenção do grau de Doutor.
Resumo: O câncer de mama é o mais comum entre as mulheres, representando uma elevada taxa de mortalidade no mundo. O carcinoma ductal invasivo (CDI) é um grupo heterogêneo de tumores com diferentes desordens em nível molecular, ainda assim foram estabelecidos cinco subgrupos (Luminal A, Luminal B, HER-enriquecido, Basal e normal-like) os quais apresentam distintos padrões de expressão gênica com diferentes implicações em prognóstico. Clinicamente, o fator prognóstico mais importante é o acometimento linfonodal, devido a sua influência na sobrevida das pacientes. O maior conhecimento do genoma humano tem permitido identificar grandes transcritos que não codificam proteínas e são originados de sequências intrônicas ou intergênicas do genoma (lncRNAs) e diferenças de expressão desse tipo de transcrito em tecidos específicos e em alguns tumores tem sido reportada, sugerindo um papel regulatório importante, portanto, estudos nessa área seriam promissores para o melhor entendimento do câncer. Nesse contexto, o presente estudo tem como objetivo avaliar a expressão de transcritos codificadores e não-codificadores de proteínas nas células epiteliais tumorais de carcinoma ductal invasivo de mama com ou sem linfonodos acometidos. A determinação da expressão gênica foi avaliada pela hibridização em uma lâmina de microarray customizada com sondas para transcritos codificadores de proteínas e transcritos longos não codificadores. Hibridizamos o RNA amplificado, proveniente de células epiteliais capturadas a laser, de 103 amostras de carcinoma ductal invasivo de mama, com e sem linfonodos acometidos, representativas dos subgrupos moleculares, caracterizados por marcadores imunoistoquímicos. A avaliação dos transcritos codificadores foi usada com dois propósitos. O primeiro foi a validação do ensaio microarray pela replicação do conjunto de genes PAM50...

Breast cancer is the most common cancer among women with high mortality rate worldwide. Although Invasive ductal carcinoma (IDC) is a heterogeneous group of tumors with different disorders at the molecular level, it has been established five subgroups (Luminal A, Luminal B, HER-enriched, basal and normal-like) that have distinct patterns of gene expression with different implications for prognosis. Clinically the most important prognostic factor is lymph node involvement due to its influence on survival of patients. Greater knowledge of the human genome has allowed to identify large transcripts that do not code for proteins and are sourced from intronic and intergenic sequences of the genome (lncRNAs), analyzes of this type of transcript has shown expression differences in specific tissues and some tumors, showing that studies in this field would be promising for a better understanding of cancer. In this context, this study aims to evaluate the expression of coding and non-coding transcripts of proteins in tumor epithelial cells of breast invasive ductal carcinoma with or without lymph nodes metastases. The determination of gene expression was assessed by hybridization on a customized microarray platform containing probes for coding and non-coding protein transcripts. We employed for hybridization the amplified RNA derived from epithelial cells captured by microdissection laser of 103 samples of invasive ductal breast carcinoma, with or without affected lymph nodes, representative of molecular subgroups, characterized by immunohistochemistry markers. The evaluation of coding transcripts was used for two purposes...
Descritores: Células Epiteliais
Análise em Microsséries
Carcinoma Ductal de Mama
Perfilação da Expressão Gênica
Prognóstico
Limites: Humanos
Responsável: BR30.1 - Biblioteca
BR30.1



página 1 de 7 ir para página                  
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde