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Pesquisa : E05.940 [Categoria DeCS]
Referências encontradas : 173 [refinar]
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Id: biblio-1041466
Autor: Oliveira, Flávio Augusto de Souza; Passarini, Guilherme Matos; Medeiros, Daniel Sol Sol de; Santos, Ana Paula de Azevedo; Fialho, Saara Neri; Gouveia, Aurileya de Jesus; Latorre, Marcinete; Freitag, Elci Marlei; Medeiros, Patrícia Soares de Maria de; Teles, Carolina Bioni Garcia; Facundo, Valdir Alves.
Título: Antiplasmodial and antileishmanial activities of compounds from Piper tuberculatum Jacq fruits
Fonte: Rev. Soc. Bras. Med. Trop;51(3):382-386, Apr.-June 2018. tab, graf.
Idioma: en.
Resumo: Abstract INTRODUCTION This study assessed the activity of compounds from Piper tuberculatum against Plasmodium falciparum and Leishmania guyanensis. METHODS The effects of compounds from P. tuberculatum fruits on P. falciparum and L. guyanensis promastigote growth in vitro were determined. Hemolytic action and cytotoxicity in HepG2 and J774 cells were measured. RESULTS Three compounds showed strong antiplasmodial activity and one compound showed strong antileishmanial activity. Two compounds were non-toxic to HepG2 cells and all were toxic to J774 cells. The compounds showed no hemolytic activity. CONCLUSIONS The tested compounds from P. tuberculatum exhibited antiparasitic and cytotoxic effects.
Descritores: Plasmodium falciparum/efeitos dos fármacos
Extratos Vegetais/farmacologia
Leishmania guyanensis/efeitos dos fármacos
Piper/química
Frutas/química
Antiprotozoários/farmacologia
-Testes de Toxicidade
Concentração Inibidora 50
Células Hep G2/efeitos dos fármacos
Antiprotozoários/isolamento & purificação
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1132155
Autor: Brustulim, Libardone José Ribeiro; Monteiro, Luciane Mendes; Almeida, Valter Paes de; Raman, Vijayasankar; Maia, Beatriz Helena Lameiro de Noronha Sales; Casapula, Inaiara; Paludo, Kátia Sabrina; Bussade, Julia Emília; Rehman, Junaid Ur; Kahn, Ikhlas Ahmed; Farago, Paulo Vitor; Budel, Jane Manfron.
Título: Ocotea porosa: Anatomy and Histochemistry of Leaves and Stems, Chemical Composition, Cytotoxicity and Insecticidal Activities of Essential Oil
Fonte: Braz. arch. biol. technol;63:e20190082, 2020. tab, graf.
Idioma: en.
Projeto: USDA-Discovery & Development of Natural Products.
Resumo: Abstract Ocotea porosa (Nees & Mart.) Barroso, commonly known as "imbuia", "canela-imbuia" or "imbuia-amarela" in Brazil, is a tree of the Southern Atlantic Forest. The present study investigates the anatomy of leaf and stem, volatile oil chemistry, as well as cytotoxicity and insecticidal activities of the essential oil of O. porosa. Species identification was achieved by anatomy features, mainly due to paracytic and anomocytic stomata; non-glandular trichomes; biconvex midrib and petiole with a collateral open arc vascular bundle; presence of a sclerenchymatous layer, starch grains and crystal sand in the stem; and the presence of phenolic compounds in the epidermis, phloem and xylem of the midrib, petiole and stem. The main volatile components of the essential oil were α-pinene (19.71%), β-pinene (13.86%) and bicyclogermacrene (24.62%). Cytotoxicity against human cancer cell (MCF-7), mouse cancer cell (B16F10) and mouse non-tumoral cell (McCoy) was observed as well as insecticidal activity of the essential oil against susceptible 'Ft. Dix' bed bugs (Cimex lectularius L.) by topical application.
Descritores: Percevejos-de-Cama
Óleos Voláteis/farmacologia
Ocotea/anatomia & histologia
Ocotea/química
Inseticidas/farmacologia
-Testes de Toxicidade
Caules de Planta/química
Folhas de Planta/química
Histocitoquímica
Responsável: BR1.1 - BIREME


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Id: biblio-1132195
Autor: Bahia, Marina; Borges, Tatiane Aparecida; Passos, Fabiana; Aquino, Sérgio Francisco de; Silva, Silvana de Queiroz.
Título: Evaluation of a Combined System Based on an Upflow Anaerobic Sludge Blanket Reactor (UASB) and Shallow Polishing Pond (SPP) for Textile Effluent Treatment
Fonte: Braz. arch. biol. technol;63:e20180130, 2020. graf.
Idioma: en.
Projeto: Minas Gerais Research Foundation.
Resumo: Abstract Color removal from textile effluents was evaluated using a laboratory-combined process based on an upflow anaerobic sludge blanket (UASB) reactor followed by a shallow polishing pond (SPP). The anaerobic reactor was fed with a real textile effluent, diluted 10-times in a 350 mg/L solution of pre-treated residual yeast extract from a brewery industry as nutrient source. The parameters color, COD, N-NH3 and toxicity were monitored throughout 45 days of operation. According to the results, decolorization and COD removal were highest in the anaerobic step, whereas the effluent was polished in the SPP unit. The overall efficiency of the complete UASB-SPP system for COD and color were 88 and 62%, respectively. Moreover, the N-NH3 generated by the residual yeast extract ammonification was below 5 mg/L for the final effluent. Finally, no toxicity was detected after the treatment steps, as shown by the Vibrio fischeri microscale assay.
Descritores: Têxteis/toxicidade
Eliminação de Resíduos Líquidos/métodos
Purificação da Água/métodos
-Leveduras
Testes de Toxicidade
Reatores Biológicos
Aliivibrio fischeri
Anaerobiose
Limites: Animais
Responsável: BR1.1 - BIREME


  4 / 173 LILACS  
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Id: biblio-1132211
Autor: Kantawong, Fahsai; Jearasakwattana, Thananat; Nira, Alisa; Chewae, Jumaila; Sajjamongkol, Phusanisa; Phothong, Pajaree; E-kobon, Teerasak; Chumnanpuen, Pramote.
Título: PI3K/Akt Signaling Involved with Osteoinductive Effects of Achatina fulica Mucus
Fonte: Braz. arch. biol. technol;63:e20180501, 2020. graf.
Idioma: en.
Resumo: Abstract Mesenchymal stem cells and osteoblasts play important roles in bone formation. Achatina fulica mucus presented the property of osteoinduction. This study aimed to examine the effects of A. fulica mucus on human mesenchymal stem cell (hMSC) and human fetal osteoblastic cell line (HFOB) differentiation. The integrated effects of A. fulica mucus and polycaprolactone (PCL) on the differentiation of hMSCs were tested. The cell viability of hMSCs treated with A. fulica mucus was investigated by the MTT assay. The cell mineralization was observed by Alizarin Red S staining, the gene expression was investigated using RT-PCR, and the PI3K activation was studied using flow cytometry. The results indicated that A. fulica mucus induced osteogenic differentiation in hMSCs and HFOBs by upregulation of the osteogenic markers; osteopontin (OPN) and osteocalcin (OCN). The results of the Alizarin Red S staining indicated that A. fulica mucus supported mineralization in both hMSCs and HFOBs. The hMSCs cultured on PCL supplemented with A. fulica mucus showed significantly increased RUNX2 and OPN expressions. A. fulica mucus was observed to increase PI3K activation in hMSCs. The findings of this study suggested that A. fulica mucus and biomaterials could be applied together for use in bone regeneration in the future.
Descritores: Osteogênese/fisiologia
Regeneração Óssea
Células-Tronco Mesenquimais/citologia
Moluscos/química
Muco/química
-Testes de Toxicidade
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Citometria de Fluxo
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME


  5 / 173 LILACS  
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Id: biblio-1132219
Autor: Rudnik, Loanda Aparecida Cabral; Lyra, Amanda Martinez; Barboza, Fernanda Malaquias; Klein, Traudi; Kanunfre, Carla Cristine; Farago, Paulo Vitor; Zanin, Sandra Maria Warumby; Nadal, Jessica Mendes.
Título: PEG-PCL Nanocapsules Containing Curcumin: Validation of HPLC Method for Analyzing Drug-loading Efficiency, Stability Testing and Cytotoxicity on NIH-3T3 Cell Line
Fonte: Braz. arch. biol. technol;63:e20200234, 2020. tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Pesquisa e Desenvolvimento.
Resumo: Abstract Curcumin (CUR) shows potential use for treating cancer. However, CUR has low solubility and reduced bioavailability, which limit its clinical effect. Therefore, the development of nanocarriers can overcome these problems and can ensure the desired pharmacological effect. In addition, it is mandatory to prove the quality, the efficacy, and the safety for a novel nanomedicine to be approved. In that sense, this paper aimed (a) to prepare CUR-loaded polyethylene glycol-poly(ε-caprolactone) nanocapsules; (b) to validate an analytical method by high performance liquid chromatography (HPLC) for quantifying CUR in these nanoformulations; (c) to evaluate the physicochemical stability of these formulations; and to investigate their cytotoxicity on NIH-3T3 mouse fibroblast cells. The HPLC method was specific to CUR in the loaded nanocapsules, linear (r = 0.9994) in a range of 10.0 to 90.0 µg.mL-1 with limits of detection and quantification of 0.160 and 0.480 µg.mL-1, respectively. Precision was demonstrated by a relative standard deviation lower than 5%. Suitable accuracy (102.37 ± 0.92%) was obtained. Values of pH, particle size, polydispersity index, and zeta potential presented no statistical difference (p > 0.05) for CUR-loaded nanoparticles. No cytotoxicity was observed against NIH-3T3 mouse embryo fibroblast cell line using both the tetrazolium salt and sulforhodamine B assays. In conclusion, a simple and inexpensive HPLC method was validated for the CUR quantification in the suspensions of nanocapsules. The obtained polymeric nanocapsules containing CUR showed suitable results for all the performed assays and can be further investigated as a feasible novel approach for cancer treatment.
Descritores: Curcumina/farmacologia
Células-Tronco Embrionárias/efeitos dos fármacos
Fibroblastos/efeitos dos fármacos
-Cromatografia Líquida de Alta Pressão
Testes de Toxicidade
Nanotecnologia
Células NIH 3T3
Embrião de Mamíferos/citologia
Nanocápsulas
Limites: Animais
Camundongos
Responsável: BR1.1 - BIREME


  6 / 173 LILACS  
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Id: biblio-1132221
Autor: Duran, Gülay Gülbol; Küçük, Meral Urhan; Algül, Öztekin; Terzi, Menderes Yusuf.
Título: Investigation of New Benzimidazole Derivative Compounds Effects on A549 Cell Line
Fonte: Braz. arch. biol. technol;63:e20190364, 2020. tab, graf.
Idioma: en.
Projeto: Hatay Mustafa Kemal University Scientific Research Projects Funding.
Resumo: Abstract Chronic inflammation is a common indication of several diseases, e.g. asthma, chronic obstructive pulmonary disease (COPD), atherosclerosis, etc. Benzimidazole derivatives are preferable compounds to design new analgesic and anti-inflammatory substances due to their unique biological features. We aimed to investigate the effect of a newly synthesized benzimidazole derivative, ORT-83, on A549 human lung adenocarcinoma cell line. ORT-83 was synthesized, and a non-cytotoxic concentration of ORT-83 on A549 cells was detected with MTT assay. To analyze the anti-inflammatory effect of ORT-83, an inflammatory cell culture model was established by stimulating A549 cell line with IL1-β (10 ng/ml). After 2 hours of treatment with IL1-β to induce inflammation, A549 cells were exposed to ORT-83 (0.78 µg/ml) for 24 hours. Thereafter gene expression analyses were performed with qRT-PCR. We found that ORT-83 significantly suppressed the gene expression levels of the proinflammatory cytokines; IL-6, NFkB, and TNF-α. However, the increased levels of IL-10 (2.8 folds) by IL-1β induction did not change after ORT-83 and/or dexamethasone (Dex: positive control) treatments. While Dex; a COX-2 inhibitor, reduced the COX-2 expression level in inflammatory cells from 10.03 folds to 0.71 folds, ORT-83 reduced its level to 4.37 folds. iNOS expression levels did not change in any experimental groups. In conclusion, we showed that ORT-83 exerted its anti-inflammatory effects by repressing the gene expression of proinflammatory cytokines in the inflammation-induced A549 cell line. Although ORT-83 had a weaker COX-2 inhibitory effect compared to Dex, it was shown to be still a strong anti-inflammatory compound.
Descritores: Benzimidazóis/farmacologia
Drogas em Investigação
Analgésicos/farmacologia
Anti-Inflamatórios/farmacologia
-Sobrevivência Celular/efeitos dos fármacos
Testes de Toxicidade
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Células A549
Limites: Humanos
Responsável: BR1.1 - BIREME


  7 / 173 LILACS  
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Id: biblio-1132255
Autor: Pegorin, Giovana SantAna; Marques, Márcia Ortiz Mayo; Mayer, Cassia Roberta Malacrida; Santos, Lucinéia.
Título: Development of a Phytocosmetic Enriched with Pequi (Caryocar brasiliense Cambess) Oil
Fonte: Braz. arch. biol. technol;63:e20190478, 2020. graf.
Idioma: en.
Resumo: Abstract The pulp oil of Caryocar brasiliense Camb., better known as pequi, is used in the typical cuisine of the Brazilian Cerrado region. It is also used in folk medicine to combat several types of disease of the respiratory system and skin. However, since its exploration is purely extractive, the exhaustion of this plant is already foreseen. Thus, in order to establish the sustainable use of pequi and contribute to its maintenance, this study aimed to develop a phytocosmetic with antioxidant and photoprotective properties using the oil of this fruit. Initially, the cytotoxicity of the oil was evaluated in order to establish the safety of its use and its fatty acid composition. Then, from the cream enriched with the oil, it was evaluated the antioxidant and photoprotector potentials, quantified the total phenolic content and examined the quality of the formulation. Pequi oil showed high percentages of palmitic (52.11%) and oleic (44.57%) fatty acids and absence of cytotoxicity. The analysis of the cream revealed 168.8 mg of total phenols in gallic acid equivalent per 100 g of oil. The evaluation of antioxidant activity showed an EC50 of 2.921 mg/mL and a capacity of inhibiting the lipoperoxidation process higher than 100%. The obtained sun protection factor was 11.40 at the concentration of 6.25 mg/mL. The quality tests revealed small disturbances in the cream stability that can be solved by further research and improvement of the formulation. The pequi oil can be converted into a phytocosmetic of great commercial value.
Descritores: Protetores Solares/análise
Óleos Vegetais/química
Cosméticos/química
Ericales/química
-Testes de Toxicidade
Compostos Fitoquímicos
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1132236
Autor: Ribeiro, Mariana Martinelli Junqueira; Silva, Karla Marins Mattos da; Palavecino, Louise Azulay; Pinto, Laine Celestino; Ferreira, Bruno Leal Alves; Lobão, Adriana Quintella; Castro, Helena Carla; Montenegro, Raquel Carvalho; Barros, Claudia Franca; Joffily, Ana; Valverde, Alessandra Leda; Paiva, Selma Ribeiro de.
Título: Anatomical, Histochemical and Biological Studies of Clusia grandiflora Splitg. (Clusiaceae)
Fonte: Braz. arch. biol. technol;63:e20190674, 2020. tab, graf.
Idioma: en.
Projeto: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; . Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro.
Resumo: Abstract Clusia grandiflora belongs to an important botanical family which is known for its medicinal value, however there are few reports in literature about the species, highlighting the relevance of this study. Anatomical studies with leaves and stems were performed using traditional techniques. In this investigation it was identified particularities of the species such as the presence of vascular system arranged in an opened arc-shaped with the flexed ends towards the inside of the arch with accessory bundles in the petiole. In histochemical studies, performed with different reagents, alkaloids, phenols substances, carbohydrates and lipids were located. The cytotoxic activity of the extracts was performed by tetrazole salt and showed promising results for ethanolic extracts of stems (IC50 human colon cells of 24.30 μg/ mL) and leaves (IC50 ascites gastric cells of 44.15 μg/ mL), without cell membrane disruption of erythrocytes. The antibacterial activity was evaluated by tryptic soy agar and minimal inhibitory concentration assays and showed positive results for Pseudomonas aeruginosa and Escherichia coli, with better result for adventitious roots (32 μg/ mL and 16 μg/ mL, respectively), stems (64 μg/ mL and 32 μg/ mL, respectively) and leaves (64 μg/ mL and 32 μg/ mL, respectively) ethanolic extracts. Thus, these studies were able to characterize the species and show its potential as promising source of active substances.
Descritores: Extratos Vegetais/farmacologia
Clusia/anatomia & histologia
Clusia/química
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Antibacterianos/farmacologia
-Testes de Toxicidade
Linhagem Celular Tumoral
Dose Letal Mediana
Antibacterianos/isolamento & purificação
Responsável: BR1.1 - BIREME


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Id: biblio-1003343
Autor: Andrade-Zuñiga, Erick M; Morales, Miguel; Ariano-Sánchez, Daniel.
Título: Toxicity of the feathers of Yellow Grosbeak, Pheucticus chrysopeplus (Passeriformes: Cardinalidae), a chemically defended neotropical bird / Toxicidad de las plumas del Picogrueso amarillo, Pheucticus chrysopeplus (Passeriformes: Cardinalidae), un ave neotropical con defensa química
Fonte: Rev. biol. trop;66(4):1530-1535, oct.-dic. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Chemical defense is a widespread mechanism on many animals and plants. However, just a few cases are known for avian species. In this study we evaluate the toxicity of Pheucticus chrysopeplus feather extract via lethality test with brine shrimp (Artemia salina) as an in vivo model. Mortality of A. salina was evaluated after 24 hour exposure to artificial seawater, methanol, and the methanolic feather extract. Kruskal-Wallis test showed a significant difference in mortality between treatments (X2 = 65.25, P < 0.0001, n = 50). With this we describe P. chrysopeplus as the first known toxic avian species of Guatemala and Central America, raising awareness about its conservation and the identification of the toxic substance present in its feathers. We also highlight the possible mimicry mechanism taking part between P. chrysopeplus and two sympatric oriole species (Icterus pectoralis and I. pustulatus).(AU)

Resumen La defensa química es un mecanismo que se encuentra presente en varios animales y plantas. Sin embargo, pocos casos son conocidos para especies de aves. En este estudio evaluamos la toxicidad de extractos de plumas de Pheucticus chrysopeplus con un ensayo de letalidad utilizando artemia (Artemia salina) como modelo in vivo. La mortalidad de A. salina se evaluó luego de ser expuesta por 24 horas a agua marina artificial, metanol y extracto metanólico de plumas de P. chrysopeplus. La prueba de Kruskal-Wallis mostró que existe una diferencia significativa entre los porcentajes de mortalidad de los tratamientos evaluados (X2 = 65.25, P < 0.0001, n = 50). Con esto, describimos a P. chrysopeplus como la primera especie de ave tóxica reportada para Guatemala y Centroamérica, resaltando la importancia de su conservación, así como la identificación de la sustancia tóxica presente en sus plumas. También destacamos el posible mecanismo de mimetismo que podría estar ocurriendo entre P. Chrysopeplus y dos especies simpátricas de orioles (Icterus pectoralis e I. pustulatus).(AU)
Descritores: Artemia
Aves
Plumas
Mimetismo Biológico
-Mortalidade
Testes de Toxicidade/instrumentação
Guatemala
Limites: Animais
Responsável: BR1.1 - BIREME


  10 / 173 LILACS  
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Id: lil-767268
Autor: Silva, EMF; Nascimento, RBC; Barreto, FS; Moraes Filho, MO; Griz, SAS; Santos, AF; Mousinho, KC.
Título: Estudo in vitro do potencial citotóxico da Annona muricata L / In vitro study of the cytotoxic potential of Annona muricata L
Fonte: Rev. ciênc. farm. básica apl;36(2), jun. 2015. tab.
Idioma: pt.
Resumo: O presente trabalho tem como objetivo avaliar a atividade citotóxica do extrato etanólico da casca do caule (AMC) e folha (AMF) da Annona muricata Linn. Para a realização desse estudo, inicialmente foi verificada a atividade do extrato etanólico nas concentrações de 1000, 800, 600, 200 e 100 ?gmL-1 para AMF e concentrações de 200, 150, 100, 50, 10 ?gmL-1 para AMC através do ensaio de Artemia salina Leach que é considerado um bioensaio preliminar no estudo de extratos com forte atividade biológica e permite realizar a avaliação da toxicidade envolvendo apenas um parâmetro: vida ou morte. Posteriormente foi realizado o ensaio de citotoxicidade através do método do MTT (3-(4,5-dimetiltiazol-2yl)-2,5-difenil brometo de tetrazolina em linhagens de SF-295 (glioblastoma - humano), OVCAR-8 (ovário) HCT-116 (colón) e HL-60 (leucemia pormielocítica). Os extratos foram testados na concentração de 50 ?g/mL para o teste de citotoxicidade de concentração única para verificar ausência ou presença de atividade. Para a determinação da concentração inibitória (CI50), todas as amostras foram testadas em concentrações seriadas que variaram de 0,09 a 50 ?g/mL utilizando 2 como fator de diluição. No presente estudo, as duas amostras utilizadas através do ensaio de Artemia salina Leach apresentaram concentração letal (CL50) superiores a 80 ?gmL-1. A folha apresentou CL50 = 324, 07?gmL-1 e a casca do caule apresentou CL50 = 196, 04?gmL-1. Já através do teste do MTT os valores de concentração inibitória (CI50) variaram de 12,81 a 22,65 ?g/mL para AMF e de 0,09 a <5 ?g/mL para AMC, frente as diferentes linhagens tumorais avaliadas. Diante dos resultados obtidos para a casca e folhas de A. muricata, avaliadas neste trabalho através dos bioensaios de toxicidade com Artemia salina Leach e com as células tumorais SF- 295, OVCAR-8, HCT-116 e HL-60, pode-se verificar o potencial tóxico de ambas as amostras, destacando-se mais as cascas que tiveram um potencial citotóxico maior. Devido a este fato, novos experimentos devem ser conduzidos para investigar melhor o potencial antitumoral das cascas do caule de A. muricata .(AU)

This study aims to evaluate the cytotoxic activity of the ethanol extract of the stem bark (AMC) and leaf (MFA) of Annona muricata Linn. To conduct this study was initially verified the activity of the ethanol extract at concentrations of 1000, 800 , 600 , 200 and 100 ?gmL-1 for MFA and concentrations of 200 , 150 , 100 , 50 , 10 ?gmL-1 for AMC through assay of Artemia salina Leach which is considered a preliminary study on bioassay of extracts with strong biological activity. Subsequently, the cytotoxicity assay was performed using the MTT (method 3 - (4,5 -dimethylthiazol- 2YL ) -2,5- diphenyl bromide in tetrazolina lines SF- 295 (glioblastoma - human), OVCAR -8 (ovarian) HCT -116 (colon) and HL -60 (promyelocytic leukemia). The extracts were tested at a concentration of 50 ?g/mL for the single concentration cytotoxicity test to determine the presence or absence of activity. For determination of inhibitory concentration (IC50), all samples were tested in serial concentrations ranging from 0, 09 to 50 ?g/mL using 2 as the dilution factor. The present study, both samples used by testing Artemia salina Leach had higher LC50 80 ?gmL-1. Sheet presented LC50 = 324, 07 ?gmL-1 and stem bark showed LC50 = 196, 04 ?gmL-1. Already through MTT inhibitory concentration values (IC50) ranged from 12, 81 to 22,65 ?g/mL for MFA and from 0,09 to <5?g/ mL for AMC, evaluated against various tumor cell lines. Results obtained for the bark and leaves of A. muricata, evaluated in this work through toxicity bioassays with Artemia salina L and tumor cells SF-295, OVCAR-8, HCT-116 and HL-60, we can verify the toxic potential of both samples, especially more hulls which had a higher cytotoxic potential. Due to this fact, further experiments should be conducted to further investigate the antitumor potential of the stem bark of A. muricata.(AU)
Descritores: Testes de Toxicidade
Annona/toxicidade
-Plantas Medicinais
Técnicas In Vitro
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação



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