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Pesquisa : E07.115 [Categoria DeCS]
Referências encontradas : 102 [refinar]
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  1 / 102 LILACS  
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Id: biblio-1087710
Autor: Guerrero, Karlo; Arancibia, Alejandra; Caceres, Manuel; Aroca, German.
Título: Release of formaldehyde during the biofiltration of methanol vapors in a peat biofilter inoculated with Pichia pastoris GS115
Fonte: Electron. j. biotechnol;44:58-59, Mar. 2020. ilus.
Idioma: en.
Resumo: BACKGROUND: Methanol can be effectively removed from air by biofiltration (Shareefdeen et al., 1993; Babbitt et al., 2009 [1,2]). However, formaldehyde is one of the first metabolic intermediates in the consumption of methanol in methylotrophic microorganisms (Negruta et al., 2010 [3]), and it can be released out of the cell constituting a secondary emission. RESULTS: The total removal of methanol was achieved up to input loads of 263 g m−3 h−1 and the maximum elimination capacity of the system was obtained at an empty bed residence times of 90 s and reached 330 g m−3 h−1 at an input methanol load of 414 g m−3 h−1 and 80% of removal efficiency. Formaldehyde was detected inside the biofilter when the input methanol load was above 212 g m−3 h−1 . Biomass in the filter bed was able to degrade the formaldehyde generated, but with the increase of the methanol input load, the unconsumed formaldehyde was released outside the biofilter. The maximum concentration registered at the output of the system was 3.98 g m−3 when the methanol load was 672 g m−3 h−1 in an empty bed residence times of 60 s. CONCLUSIONS: Formaldehyde is produced inside a biofilter when methanol is treated in a biofiltration system inoculated with Pichia pastoris. Biomass present in the reactor is capable of degrading the formaldehyde generated as the concentration of methanol decreases. However, high methanol loads can lead to the generation and release of formaldehyde into the environment.
Descritores: Pichia/química
Metanol/química
Formaldeído/análise
-Volatilização
Filtros Biológicos
Biomassa
Reatores Biológicos
Meio Ambiente
Responsável: CL1.1 - Biblioteca Central


  2 / 102 LILACS  
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Id: biblio-1051568
Autor: Bavandi, Roya; Emtyazjoo, Mozhgan; Nasrollahzadeh Saravi, Hasan; Yazdian, Fatemeh; Sheikhpour, Mojgan.
Título: Study of capability of nanostructured zero-valent iron and graphene oxide for bioremoval of trinitrophenol from wastewater in a bubble column bioreactor
Fonte: Electron. j. biotechnol;39:8-14, may. 2019. tab, graf, ilus.
Idioma: en.
Resumo: BACKGROUND: Bioremoval of phenolic compounds using fungi and bacteria has been studied extensively; nevertheless, trinitrophenol bioremediation using modified Oscillatoria cyanobacteria has been barely studied in the literature. RESULTS: Among the effective parameters of bioremediation, algal concentration (3.18 g·L−1 ), trinitrophenol concentration (1301 mg·L−1 ), and reaction time (3.75 d) were screened by statistical analysis. Oscillatoria cyanobacteria were modified by starch/nZVI and starch/graphene oxide in a bubble column bioreactor, and their bioremoval efficiency was investigated. Modifiers, namely, starch/zero-valent iron and starch/GO, increased trinitrophenol bioremoval efficiency by more than 10% and 12%, respectively, as compared to the use of Oscillatoria cyanobacteria alone. Conclusions: It was found that starch/nano zero-valent iron and starch/GO could be applied to improve the removal rate of phenolic compounds from the aqueous solution.
Descritores: Picratos/metabolismo
Oscillatoria/metabolismo
-Picratos/análise
Amido
Biodegradação Ambiental
Reatores Biológicos
Compostos Fenólicos/análise
Nanopartículas Metálicas
Águas Residuárias
Grafite
Ferro
Responsável: CL1.1 - Biblioteca Central


  3 / 102 LILACS  
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Id: biblio-1053462
Autor: Panyachanakul, Titiporn; Sorachart, Bodeesorn; Lumyong, Saisamorn; Lorliam, Wanlapa; Kitpreechavanich, Vichien; Krajangsang, Sukhumaporn.
Título: Development of biodegradation process for Poly(DL-lactic acid) degradation by crude enzyme produced by Actinomadura keratinilytica strain T16-1
Fonte: Electron. j. biotechnol;40:52-57, July. 2019. graf, tab.
Idioma: en.
Projeto: Srinakharinwirot University research fund, Thailand; . Center of Excellence on Biodiversity (BDC), office of higher education commission, Thailand.
Resumo: Background: Plastic waste is a serious problem because it is difficult to degrade, thereby leading to global environment problems. Poly(lactic acid) (PLA) is a biodegradable aliphatic polyester derived from renewable resources, and it can be degraded by various enzymes produced by microorganisms. This study focused on the scale-up and evaluated the bioprocess of PLA degradation by a crude microbial enzyme produced by Actinomadura keratinilytica strain T16-1 in a 5 L stirred tank bioreactor. Results: PLA degradation after 72 h in a 5 L bioreactor by using the enzyme of the strain T16-1 under controlled pH conditions resulted in lactic acid titers (mg/L) of 16,651 mg/L and a conversion efficiency of 89% at a controlled pH of 8.0. However, the PLA degradation process inadvertently produced lactic acid as a potential inhibitor, as shown in our experiments at various concentrations of lactic acid. Therefore, the dialysis method was performed to reduce the concentration of lactic acid. The experiment with a dialysis bag achieved PLA degradation by weight loss of 99.93%, whereas the one without dialysis achieved a degradation of less than approximately 14.75%. Therefore, the dialysis method was applied to degrade a commercial PLA material (tray) with a conversion efficiency of 32%, which was 6-fold more than that without dialysis. Conclusions: This is the first report demonstrating the scale-up of PLA degradation in a 5 L bioreactor and evaluating a potential method for enhancing PLA degradation efficiency.
Descritores: Poliésteres/metabolismo
Actinomycetales/enzimologia
Enzimas/metabolismo
-Polímeros/metabolismo
Biodegradação Ambiental
Ácido Láctico/análise
Reatores Biológicos
Concentração de Íons de Hidrogênio
Responsável: CL1.1 - Biblioteca Central


  4 / 102 LILACS  
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Id: biblio-1087631
Autor: Yuan, Wei; Lin, Xiaoqiong; Zhong, Shuang; Chen, Junren; Wang, Zhao; Sun, Jie.
Título: Enhanced pyruvic acid yield in an osmotic stress-resistant mutant of Yarrowia lipolytica
Fonte: Electron. j. biotechnol;44:19-24, Mar. 2020. ilus, graf.
Idioma: en.
Projeto: Zhejiang Provincial Natural Science Foundation of China; . National Natural Science Foundation of China.
Resumo: BACKGROUND: Pyruvic acid (PA), a vital α-oxocarboxylic acid, plays an important role in energy and carbon metabolism. The oleaginous yeast Yarrowia lipolytica (Y. lipolytica) has considerable potential for the production of PA. An increased NaCl concentration reportedly increases the biomass and PA yield of Y. lipolytica. RESULTS: To increase the yield of PA, the NaCl-tolerant Y. lipolytica A4 mutant was produced using the atmospheric and room temperature plasma method of mutation. The A4 mutant showed growth on medium containing 160 g/L NaCl. The PA yield of the A4 mutant reached 97.2 g/L at 120 h (0.795 g/g glycerol) in a 20-L fermenter with glycerol as the sole carbon source, which was 28.9% higher than that of the parental strain. CONCLUSION: The PA yield from Y. lipolytica can be improved by increasing its NaCl tolerance.
Descritores: Ácido Pirúvico/metabolismo
Yarrowia/genética
Yarrowia/metabolismo
-Pressão Osmótica
Leveduras
Carbono/metabolismo
Cloreto de Sódio
Reatores Biológicos
Tolerância ao Sal/genética
Fermentação
Glicerol/metabolismo
Mutação
Responsável: CL1.1 - Biblioteca Central


  5 / 102 LILACS  
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Texto completo SciELO Brasil
Texto completo
Id: biblio-974307
Autor: Zhang, Nan; Peng, Huijuan; Li, Yong; Yang, Wenxiu; Zou, Yuneng; Duan, Huiguo.
Título: Ammonia determines transcriptional profile of microorganisms in anaerobic digestion
Fonte: Braz. j. microbiol;49(4):770-776, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Key Project of Education Department of Sichuan Province; . Major Cultivation Project of Education Department of Sichuan Province.
Resumo: ABSTRACT Anaerobic digestion is important for the management of livestock manure with high ammonia level. Although ammonia effects on anaerobic digestion have been comprehensively studied, the molecular mechanism underlying ammonia inhibition still remains elusive. In this study, based on metatranscriptomic analysis, the transcriptional profile of microbial community in anaerobic digestion under low (1500 mg L-1) and high NH4 + (5000 mg L-1) concentrations, respectively, were revealed. The results showed that high NH4 + concentrations significantly inhibited methane production but facilitated the accumulations of volatile fatty acids. The expression of methanogenic pathway was significantly inhibited by high NH4 + concentration but most of the other pathways were not significantly affected. Furthermore, the expressions of methanogenic genes which encode acetyl-CoA decarbonylase and methyl-coenzyme M reductase were significantly inhibited by high NH4 + concentration. The inhibition of the co-expressions of the genes which encode acetyl-CoA decarbonylase was observed. Some genes involved in the pathways of aminoacyl-tRNA biosynthesis and ribosome were highly expressed under high NH4 + concentration. Consequently, the ammonia inhibition on anaerobic digestion mainly focused on methanogenic process by suppressing the expressions of genes which encode acetyl-CoA decarbonylase and methyl-coenzyme M reductase. This study improved the accuracy and depth of understanding ammonia inhibition on anaerobic digestion.
Descritores: Bactérias/genética
Bactérias/metabolismo
Amônia/metabolismo
-Bactérias/isolamento & purificação
Bactérias/classificação
Transcrição Genética
Reatores Biológicos/microbiologia
Ácidos Graxos Voláteis/metabolismo
Microbiota
Anaerobiose
Metano/metabolismo
Responsável: BR1.1 - BIREME


  6 / 102 LILACS  
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Ayub, Marco Antonio Zachia
Texto completo
Id: biblio-828200
Autor: Coghetto, Chaline Caren; Vasconcelos, Carolina Bettker; Brinques, Graziela Brusch; Ayub, Marco Antônio Záchia.
Título: Lactobacillus plantarum BL011 cultivation in industrial isolated soybean protein acid residue
Fonte: Braz. j. microbiol;47(4):941-948, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract In this study, physiological aspects of Lactobacillus plantarum BL011 growing in a new, all-animal free medium in bioreactors were evaluated aiming at the production of this important lactic acid bacterium. Cultivations were performed in submerged batch bioreactors using the Plackett-Burman methodology to evaluate the influence of temperature, aeration rate and stirring speed as well as the concentrations of liquid acid protein residue of soybean, soy peptone, corn steep liquor, and raw yeast extract. The results showed that all variables, except for corn steep liquor, significantly influenced biomass production. The best condition was applied to bioreactor cultures, which produced a maximal biomass of 17.87 g L-1, whereas lactic acid, the most important lactic acid bacteria metabolite, peaked at 37.59 g L-1, corresponding to a productivity of 1.46 g L-1 h-1. This is the first report on the use of liquid acid protein residue of soybean medium for L. plantarum growth. These results support the industrial use of this system as an alternative to produce probiotics without animal-derived ingredients to obtain high biomass concentrations in batch bioreactors.
Descritores: Proteínas de Soja
Meios de Cultura
Lactobacillus plantarum/crescimento & desenvolvimento
Lactobacillus plantarum/metabolismo
-Biomassa
Ácido Láctico/biossíntese
Proteínas de Soja/metabolismo
Proteínas de Soja/química
Reatores Biológicos
Meios de Cultura/química
Metabolismo dos Carboidratos
Fermentação
Hidrólise
Responsável: BR1.1 - BIREME


  7 / 102 LILACS  
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Texto completo SciELO Brasil
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Id: lil-788957
Autor: Barros, Valciney Gomes de; Duda, Rose Maria; Oliveira, Roberto Alves de.
Título: Biomethane production from vinasse in upflow anaerobic sludge blanket reactors inoculated with granular sludge
Fonte: Braz. j. microbiol;47(3):628-639, July-Sept. 2016. tab, graf.
Idioma: en.
Projeto: CAPES; . CNPq.
Resumo: ABSTRACT The main objective of this study was to evaluate the anaerobic conversion of vinasse into biomethane with gradual increase in organic loading rate (OLR) in two upflow anaerobic sludge blanket (UASB) reactors, R1 and R2, with volumes of 40.5 and 21.5 L in the mesophilic temperature range. The UASB reactors were operated for 230 days with a hydraulic detection time (HDT) of 2.8 d (R1) and 2.8-1.8 d (R2). The OLR values applied in the reactors were 0.2-7.5 g totalCOD (L d)−1 in R1 and 0.2-11.5 g totalCOD (L d)−1 in R2. The average total chemical oxygen demand (totalCOD) removal efficiencies ranged from 49% to 82% and the average conversion efficiencies of the removed totalCOD into methane were 48-58% in R1 and 39-65% in R2. The effluent recirculation was used for an OLR above 6 g totalCOD (L d)−1 in R1 and 8 gtotalCOD (L d)−1 in R2 and was able to maintain the pH of the influent in R1 and R2 in the range from 6.5 to 6.8. However, this caused a decrease for 53-39% in the conversion efficiency of the removed totalCOD into methane in R2 because of the increase in the recalcitrant COD in the influent. The largest methane yield values were 0.181 and 0.185 (L) CH4 (gtotal COD removed)−1 in R1 and R2, respectively. These values were attained after 140 days of operation with an OLR of 5.0-7.5 g totalCOD (L d)−1 and total COD removal efficiencies around 70 and 80%.
Descritores: Esgotos/microbiologia
Fermentação
Metano/biossíntese
-Fósforo/metabolismo
Reatores Biológicos
Compostos Orgânicos Voláteis
Análise da Demanda Biológica de Oxigênio
Concentração de Íons de Hidrogênio
Anaerobiose
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  8 / 102 LILACS  
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Texto completo
Id: biblio-839351
Autor: An, Chao; Ma, Sai-jian; Chang, Fan; Xue, Wen-jiao.
Título: Efficient production of pullulan by Aureobasidium pullulans grown on mixtures of potato starch hydrolysate and sucrose
Fonte: Braz. j. microbiol;48(1):180-185, Jan.-Mar. 2017. tab, graf.
Idioma: en.
Projeto: Scientific and Technologic Research Program of Shaanxi Academy of Sciences, China; . WesternChinese Academy of Sciences; . Natural Science Basic Research Plan in Shaanxi Province of China; . Shaanxi Science & Technology Co-ordination & Innovation Project; . Science and Technology Program of Xi’an.
Resumo: Abstract Pullulan is a natural exopolysaccharide with many useful characteristics. However, pullulan is more costly than other exopolysaccharides, which limits its effective application. The purpose of this study was to adopt a novel mixed-sugar strategy for maximizing pullulan production, mainly using potato starch hydrolysate as a low-cost substrate for liquid-state fermentation by Aureobasidium pullulans. Based on fermentation kinetics evaluation of pullulan production by A. pullulans 201253, the pullulan production rate of A. pullulans with mixtures of potato starch hydrolysate and sucrose (potato starch hydrolysate:sucrose = 80:20) was 0.212 h−1, which was significantly higher than those of potato starch hydrolysate alone (0.146 h−1) and mixtures of potato starch hydrolysate, glucose, and fructose (potato starch hydrolysate:glucose:fructose = 80:10:10, 0.166 h−1) with 100 g L−1 total carbon source. The results suggest that mixtures of potato starch hydrolysate and sucrose could promote pullulan synthesis and possibly that a small amount of sucrose stimulated the enzyme responsible for pullulan synthesis and promoted effective potato starch hydrolysate conversion effectively. Thus, mixed sugars in potato starch hydrolysate and sucrose fermentation might be a promising alternative for the economical production of pullulan.
Descritores: Ascomicetos/metabolismo
Amido/metabolismo
Sacarose/metabolismo
Solanum tuberosum/química
Fermentação
Glucanos/biossíntese
-Amido/química
Carbono/metabolismo
Cinética
Biomassa
Reatores Biológicos
Técnicas de Cultura Celular por Lotes
Responsável: BR1.1 - BIREME


  9 / 102 LILACS  
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Texto completo SciELO Chile
Texto completo
Id: lil-504112
Autor: Shohael, Abdullah Mohammad; Murthy, Hosakatte Niranjana; Hahn, Eun-Joo; Paek, Kee-Yoeup.
Título: Methyl jasmonate induced overproduction of eleutherosides in somatic embryos of Eleutherococcus senticosus cultured in bioreactors
Fonte: Electron. j. biotechnol;10(4):633-637, oct. 2007. tab.
Idioma: en.
Projeto: Ministry of Education and Human Resources Development; . Ministry of Commerce, Industry and Energy; . Ministry of Labor; . Korean Federation of Science and Technology Societies. Brain Pool Fellowship.
Resumo: This study was concentrated on the production of eleutherosides and chlorogenic acid in embryogenic suspension cultures of Eleutherococcus senticosus by exposing them to different concentrations (50-400 µM) of methyl jasmonate (MJ) during the culture period. In the bioreactor cultures, eleutheroside content increased significantly by elicitation of MJ, however, the fresh weight, dry weight and growth ratio of embryos was strongly inhibited by increasing MJ concentrations. The highest total eleutheroside (7.3 fold increment) and chlorogenic acid (3.9 fold increment) yield was obtained with 200 µM MJ treatment. There was 1.4, 3.4 and 14.9 fold increase in the eleutheroside B, E, and E1 production respectively with such elicitation treatment. These results suggest that MJ elicitation is beneficial for eleutheroside accumulation in the embryogenic cell suspension cultures.
Descritores: Eleutherococcus/metabolismo
Extratos Vegetais/análise
Reatores Biológicos
Reguladores de Crescimento de Planta/metabolismo
-Eleutherococcus/citologia
Eleutherococcus/embriologia
Membrana Celular/metabolismo
Técnicas de Cultura de Células
Responsável: CL1.1 - Biblioteca Central


  10 / 102 LILACS  
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Id: biblio-1021543
Autor: Panyachanakul, Titiporn; Kitpreechavanich, Vichien; Tokuyama, Shinji; Krajangsang, Sukhumaporn.
Título: Poly(DL-lactide)-degrading enzyme production by immobilized Actinomadura keratinilytica strain T16-1 in a 5-L fermenter under various fermentation processes
Fonte: Electron. j. biotechnol;30:71-76, nov. 2017. graf, ilus, tab.
Idioma: en.
Projeto: National Research Council of Thailand.
Resumo: Background: Poly(DL-lactic acid), or PDLLA, is a biodegradable polymer that can be hydrolyzed by various types of enzymes. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported to have PDLLA depolymerase activity. However, few studies have reported on PDLLA-degrading enzyme production by bacteria. Therefore, the aims of this study were to determine a suitable immobilization material for PDLLA-degrading enzyme production and optimize PDLLA-degrading enzyme production by using immobilized A. keratinilytica strain T16-1 under various fermentation process conditions in a stirrer fermenter. Results: Among the tested immobilization materials, a scrub pad was the best immobilizer, giving an enzyme activity of 30.03 U/mL in a shake-flask scale. The maximum enzyme activity was obtained at aeration 0.25 vvm, agitation 170 rpm, 45°C, and 48 h of cultivation time. Under these conditions, a PDLLA-degrading enzyme production of 766.33 U/mL with 15.97 U/mL·h productivity was observed using batch fermentation in a 5-L stirrer fermenter. Increased enzyme activity and productivity were observed in repeated-batch (942.67 U/mL and 19.64 U/mL·h) and continuous fermentation (796.43 U/mL and 16.58 U/mL·h) at a dilution rate of 0.013/h. Scaled-up production of the enzyme in a 10-L stirrer bioreactor using the optimized conditions showed a maximum enzyme activity of 578.67 U/mL and a productivity of 12.06 U/mL·h. Conclusions: This research successfully scaled-up the enzyme production to 5 and 10 L in a stirrer fermenter and is helpful for many applications of poly(lactic acid).
Descritores: Poliésteres/metabolismo
Actinomycetales/enzimologia
Enzimas/biossíntese
-Biodegradação Ambiental
Reatores Biológicos
Enzimas/metabolismo
Enzimas Imobilizadas
Fermentação
Responsável: CL1.1 - Biblioteca Central



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