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Pesquisa : E07.115 [Categoria DeCS]
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Id: biblio-953182
Autor: Rodrigues, Isabella Caroline Pereira; Kaasi, Andreas; Maciel Filho, Rubens; Jardini, André Luiz; Gabriel, Laís Pellizzer.
Título: Cardiac tissue engineering: current state-of-the-art materials, cells and tissue formation / Engenharia de tecidos cardíacos: atual estado da arte a respeito de materiais, células e formação tecidual
Fonte: Einstein (Säo Paulo);16(3):eRB4538, 2018. tab, graf.
Idioma: en.
Projeto: Instituto Nacional de Ciência e Tecnologia em Biofabricação (INCT-BIOFABRIS), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); . Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP).
Resumo: ABSTRACT Cardiovascular diseases are the major cause of death worldwide. The heart has limited capacity of regeneration, therefore, transplantation is the only solution in some cases despite presenting many disadvantages. Tissue engineering has been considered the ideal strategy for regenerative medicine in cardiology. It is an interdisciplinary field combining many techniques that aim to maintain, regenerate or replace a tissue or organ. The main approach of cardiac tissue engineering is to create cardiac grafts, either whole heart substitutes or tissues that can be efficiently implanted in the organism, regenerating the tissue and giving rise to a fully functional heart, without causing side effects, such as immunogenicity. In this review, we systematically present and compare the techniques that have drawn the most attention in this field and that generally have focused on four important issues: the scaffold material selection, the scaffold material production, cellular selection and in vitro cell culture. Many studies used several techniques that are herein presented, including biopolymers, decellularization and bioreactors, and made significant advances, either seeking a graft or an entire bioartificial heart. However, much work remains to better understand and improve existing techniques, to develop robust, efficient and efficacious methods.

RESUMO Doenças cardiovasculares são responsáveis pelo maior número de mortes no mundo. O coração possui capacidade de regeneração limitada, e o transplante, por consequência, representa a única solução em alguns casos, apresentando várias desvantagens. A engenharia de tecidos tem sido considerada a estratégia ideal para a medicina cardíaca regenerativa. Trata-se de uma área interdisciplinar, que combina muitas técnicas as quais buscam manter, regenerar ou substituir um tecido ou órgão. A abordagem principal da engenharia de tecidos cardíacos é criar enxertos cardíacos, sejam substitutos do coração inteiro ou de tecidos que podem ser implantados de forma eficiente no organismo, regenerando o tecido e dando origem a um coração completamente funcional, sem desencadear efeitos colaterais, como imunogenicidade. Nesta revisão, apresentase e compara-se sistematicamente as técnicas que ganharam mais atenção nesta área e que geralmente focam em quatro assuntos importantes: seleção do material a ser utilizado como enxerto, produção do material, seleção das células e cultura de células in vitro. Muitos estudos, fazendo uso de várias das técnicas aqui apresentadas, incluindo biopolímeros, descelularização e biorreatores, têm apresentado avanços significativos, seja para obter um enxerto ou um coração bioartifical inteiro. No entanto, ainda resta um grande esforço para entender e melhorar as técnicas existentes, para desenvolver métodos robustos, eficientes e eficazes.
Descritores: Transplante de Coração/métodos
Engenharia Tecidual/métodos
Miocárdio/citologia
-Biopolímeros
Transplante de Coração/tendências
Técnicas de Cultura de Células/métodos
Reatores Biológicos
Engenharia Tecidual/tendências
Tecidos Suporte
Limites: Humanos
Responsável: BR1.1 - BIREME


  2 / 113 LILACS  
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Id: biblio-1024839
Autor: Aguirre, Alberto; Bernal, Pamela; Maureira, Daniela; Ramos, Nicolás; Vásquez, Javier; Urrutia, Homero; Gentina, Juan Carlos; Aroca, Germán.
Título: Biofiltration of trimethylamine in biotrickling filter inoculated with Aminobacter aminovorans
Fonte: Electron. j. biotechnol;33:63-67, May. 2018. ilus, graf, tab.
Idioma: en.
Projeto: FONDECYT.
Resumo: Background: Trimethylamine (TMA) is the main responsible for the odor associated with rotting fish and other annoying odors generated in many industrial activities. Biofiltration has proved to be efficient for treating odorous gaseous emissions. The main objective of this work was to determine the removal capacity of TMA of a biotrickling filter inoculated with Aminobacter aminovorans and to evaluate the effect of H2S on its performance. Results: The maximumspecific growth rate ofA. aminovorans in a liquid culture was 0.15 h -1 , witha TMAto biomass yield of 0.10 (g g -1) and a specific consumption rate of 0.062 g·g-1·h-1 . The initial specific consumption rate of TMA was highly influenced by the presence of H2S in liquid culture at concentrations of 20 and 69 ppm in heading space oftheflasks.ABTF inoculatedwithA. aminovorans showedremoval efficiencieshigher than98%ina range ofloading rate of 0.2 to 8 g·m-3·h-1 at empty bed residence time (EBRT) of 85 and 180 s. No effect on the elimination capacity and efficiency was detected when H2S was added at 20 and 50 ppm to the inlet gaseous emission, though the fraction of A. aminovorans measured by qPCR in the biofilm decreased. Conclusions:Abiotrickling filter inoculated with A. aminovorans can remove efficiently the TMA in a gaseous stream. The elimination capacity of TMA can be negatively affected by H2S, but its effect is not notorious when it is forming part of a biofilm, due to its high specific consumption rate of TMA.
Descritores: Alphaproteobacteria/metabolismo
Sulfeto de Hidrogênio
Metilaminas/metabolismo
-Desodorização/métodos
Reatores Biológicos
Filtração
Peixes
Responsável: CL1.1 - Biblioteca Central


  3 / 113 LILACS  
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Id: biblio-1026812
Autor: Sulieman, Ashraf K; Putra, Meilana Dharma; Abasaeed, Ahmed E; Gaily, Mohamed H; Al-Zahrani, Saeed M; Zeinelabdeen, Mohamed A.
Título: Kinetic modeling of the simultaneous production of ethanol and fructose by Saccharomyces cerevisiae
Fonte: Electron. j. biotechnol;34:1-8, july. 2018. tab, graf.
Idioma: en.
Resumo: Background: Ethanol and fructose are two important industrial products that enjoy many uses. In this contribution, their production via selective fermentation of date extract using Saccharomyces cerevisiae was studied. Scaling up the process for possible commercialization was investigated in three fermentors with working volume ratio of 1:40:400. Results: Higher ethanol concentration was obtained in the larger fermentor due to conversion of fructose. Fructose yields in the 0.5-L, 7.5-L and 80-L fermentors were 99, 92 and 90%, respectively. Good fitting was obtained with the modified Monod kinetics; however, a better fit of cell mass was obtained with the modified Ghose­Tyagi model which accounts for ethanol inhibition. Conclusions: The modified Gompertz model was expanded to facilitate prediction of products' formation and fructose fractions in all three fermentors. Such expansion will be beneficial in industrial applications.
Descritores: Saccharomyces cerevisiae/metabolismo
Etanol/síntese química
Frutose/biossíntese
-Leveduras
Cinética
Reatores Biológicos
Fermentação
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1283600
Autor: Oestreich, Arne Michael; Ilire Suli, Merlinda; Gerlach, Doreen; Fan, Rong; Czermak, Peter.
Título: Media development and process parameter optimization using statistical experimental designs for the production of nonribosomal peptides in Escherichia coli
Fonte: Electron. j. biotechnol;52:85-92, July. 2021. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Nonribosomal peptide synthases (NRPS) can synthesize functionally diverse bioactive peptides by incorporating nonproteinogenic amino acids, offering a rich source of new drug leads. The bacterium Escherichia coli is a well-characterized production host and a promising candidate for the synthesis of nonribosomal peptides, but only limited bioprocess engineering has been reported for such molecules. We therefore developed a medium and optimized process parameters using the design of experiments (DoE) approach. RESULTS: We found that glycerol is not suitable as a carbon source for rhabdopeptide production, at least for the NRPS used for this study. Alternative carbon sources from the tricarboxylic acid cycle achieved much higher yields. DoE was used to optimize the pH and temperature in a stirred-tank reactor, revealing that optimal growth and optimal production required substantially different conditions. CONCLUSIONS: We developed a chemically defined adapted M9 medium matching the performance of complex medium (lysogeny broth) in terms of product concentration. The maximum yield in the reactor under optimized conditions was 126 mg L-1, representing a 31-fold increase compared to the first shaking-flask experiments with M9 medium and glycerol as the carbon source. Conditions that promoted cell growth tended to inhibit NRPS productivity. The challenge was therefore to find a compromise between these factors as the basis for further process development.
Descritores: Peptídeo Sintases/metabolismo
Reatores Biológicos/microbiologia
Escherichia coli
-Temperatura
Biotecnologia
Carbono/metabolismo
Modelos Estatísticos
Eletroforese em Gel de Poliacrilamida
Bioengenharia
Concentração de Íons de Hidrogênio
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1132182
Autor: Almeida, Lariana Negrão Beraldo; Pietrobelli, Juliana Martins Teixeira Abreu; Lenzi, Giane Gonçalves; Santos, Onélia Aparecida Andreo.
Título: Degradation of Caffeine by Heterogeneous Photocatalysis Using ZnO with Fe and Ag
Fonte: Braz. arch. biol. technol;63:e20180614, 2020. tab, graf.
Idioma: en.
Resumo: Abstract The organic compound caffeine when detected in environmental matrices such as surface waters and groundwater is considered as an emerging contaminant, in which its effects are still unknown. Therefore, in the present research, zinc oxide-based catalysts impregnated with iron and silver were prepared for the reaction of caffeine degradation by heterogeneous photocatalysis. The wet impregnation method with excess solvent was applied to the preparation of the materials, later they were characterized by adsorption of N2, X-ray diffraction and photoacoustic spectroscopy. Then, the photodegradation, photolysis and adsorption tests were carried out, in which it was observed that only the presence of the radiation or photocatalysts could not sufficiently degrade the caffeine, however when combined radiation with all the catalysts studied here presented degradation above 70% at the end of 300 minutes of the reaction, and the best catalyst studied was that containing 8% Ag in non-calcined ZnO. Thus, these results point out that the methodology employed in this research, both for the preparation of the catalysts and in the process of the photocatalysis reaction, was efficient in the degradation of the emerging contaminant, caffeine, which could later be used for a mixture of other contaminants.
Descritores: Prata/química
Óxido de Zinco/química
Cafeína/química
Catálise
Processos Fotoquímicos
-Adsorção
Reatores Biológicos
Ferro/química
Responsável: BR1.1 - BIREME


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Id: biblio-1132195
Autor: Bahia, Marina; Borges, Tatiane Aparecida; Passos, Fabiana; Aquino, Sérgio Francisco de; Silva, Silvana de Queiroz.
Título: Evaluation of a Combined System Based on an Upflow Anaerobic Sludge Blanket Reactor (UASB) and Shallow Polishing Pond (SPP) for Textile Effluent Treatment
Fonte: Braz. arch. biol. technol;63:e20180130, 2020. graf.
Idioma: en.
Projeto: Minas Gerais Research Foundation.
Resumo: Abstract Color removal from textile effluents was evaluated using a laboratory-combined process based on an upflow anaerobic sludge blanket (UASB) reactor followed by a shallow polishing pond (SPP). The anaerobic reactor was fed with a real textile effluent, diluted 10-times in a 350 mg/L solution of pre-treated residual yeast extract from a brewery industry as nutrient source. The parameters color, COD, N-NH3 and toxicity were monitored throughout 45 days of operation. According to the results, decolorization and COD removal were highest in the anaerobic step, whereas the effluent was polished in the SPP unit. The overall efficiency of the complete UASB-SPP system for COD and color were 88 and 62%, respectively. Moreover, the N-NH3 generated by the residual yeast extract ammonification was below 5 mg/L for the final effluent. Finally, no toxicity was detected after the treatment steps, as shown by the Vibrio fischeri microscale assay.
Descritores: Têxteis/toxicidade
Eliminação de Resíduos Líquidos/métodos
Purificação da Água/métodos
-Leveduras
Testes de Toxicidade
Reatores Biológicos
Aliivibrio fischeri
Anaerobiose
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-1132205
Autor: Campos, Fábio; Bueno, Rodrigo de Freitas; Piveli, Roque Passos.
Título: Influence of Landfill Leachate on Organic Matter Removal in Activated Sludge and Activated Sludge with Carrier Processes
Fonte: Braz. arch. biol. technol;63:e20180384, 2020. tab, graf.
Idioma: en.
Resumo: Abstract This study assessed the performance of two pilot units, a conventional activated sludge (CAS) and an Integrated Fixed-Film Activated Sludge (IAS), in the treatment of leachate from sanitary landfill combined with domestic sewage, with the aim of removing organic substances. In order to assess the possible impacts on treatment, three experimental phases were designed using influent leachate contributions of 5%, 10%, and 20% of the total BOD load. Overall, the results showed that no significant changes in the behavior of biological processes. The IAS unit presented average BOD removal efficiencies of above 88%, 87% and 80% for the three leachate load contributions of 5%, 10% and 20% studied. The CAS unit presented lower average efficiency with a 5% leachate contribution (64%), but displayed a similar performance to the IAS unit in the other phases (82% and 80%); similar quantitative aspects were observed for both treatment processes with regards to biomass composition analysis. The kinetic coefficients related to heterotrophic metabolism did not indicate negative effects on treatment efficiency as a result of the increase in leachate addition, with values of 2.8; 2.8 and 2.7 d-1 obtained respectively for each phase with the CAS unit, and 2.0; 1.5 and 1.6 d-1 with the IAS unit, representing similar values to those found in the literature (1.5 a 5.0 d-1). The results reinforced the conclusion that the leachate load introduced did not cause inhibition or significant alterations to heterotrophic metabolism, and, therefore, to the removal of organic matter.
Descritores: Poluentes Químicos da Água/metabolismo
Líquido Percolado
Lodos Ativados
Purificação da Água/métodos
Biomassa
-Projetos Piloto
Reatores Biológicos
Bactérias Heterotróficas
Matéria Orgânica
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME


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Id: biblio-1132218
Autor: Maidana, Silvana Andrea; Esteche, Vanesa Paola; Hours, Roque Alberto; Brumovsky, Luis Alberto; Martos, María Alicia.
Título: Use of Agro-Industrial Wastes for the Production of a Wild Yeast Enzyme with Disintegration Activity on Plant Tissues
Fonte: Braz. arch. biol. technol;63:e20190515, 2020. tab.
Idioma: en.
Projeto: National Interuniversity Council; . University Policy Secretary; . School of Exact and Natural Sciences, Misiones National University.
Resumo: Abstract The objective of the present study was to develop a cost-effective medium, using agro-industrial wastes for the production of a polygalacturonase by Wickerhanomyces anomalus of interest in cassava starch industries. The effect of several raw agro-industrial wastes and others nutrients on polygalacturonase production by W. anomalus, were evaluated, in a reference fermentation medium, using statistical designs, by batch culture. The ability of the cell-free supernatant to extract cassava starch was evaluated. Lemon peel was the best inducer for the production of PGase. Statistical analysis of the data showed that lemon peel, Mg+2 and PO4HK2 had significant effect on PGase production, and the others variables (yeast extract, Ca+2, Fe+2, amino acid and trace element solution) were no significant. PGase synthesis reached ~31 EUmL-1, in the OFM (glucose, lemon peel, urea, vitamins, KH2PO4 and MgSO4), after 12 h of culture, at a lab scale bioreactor. PGase of W. anomalus, was able to disintegrate cassava tuber tissue, and the starch granules contained within the cells were released into the reaction medium. Lemon peel can be used as inducer for PGase production by W. anomalus, in a low cost culture medium, appropriate for the production of the enzyme at large scale.
Descritores: Poligalacturonase/biossíntese
Reatores Biológicos
Amidos e Féculas
Resíduos Industriais
-Análise Custo-Benefício
Agricultura
Fermentação
Responsável: BR1.1 - BIREME


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Id: biblio-1132271
Autor: Bettin, Fernanda; Rosa, Letícia Osório da; Montanari, Queli; Zaccaria, Simone; Dillon, Aldo José Pinheiro; Silveira, Mauricio Moura da.
Título: Influence of Oxygen Supply on Growth and Laccases Production by Pleurotus sajor-caju PS-2001 in Submerged Process
Fonte: Braz. arch. biol. technol;63:e20190015, 2020. tab, graf.
Idioma: en.
Resumo: Abstract (1) Background: Oxygen supply is an important parameter to be considered in submerged cultures. This study evaluated the influence of different conditions for dissolved oxygen (DO) concentration on laccases activities and growth of Pleurotus sajor-caju PS-2001 in submerged process in stirred-tank bioreactor. (2) Methods: Initially, three different conditions were tested: uncontrolled DO and minimum levels of 30% and 80% of saturation, with the pH controlled between 4.5 and 7.0. (3) Results: Best results were observed at 30% DO (26 U mL-1 of laccases at 96 h), whereas higher mycelial biomass was observed at 30% and 80% DO (above 4.5 g L-1). Four different conditions of DO (uncontrolled, 10%, 30% and 50% of saturation) were tested at pH 6.5, with higher laccases activity (80 U mL-1 at 66 h) and lower mycelial growth (1.36 g L-1 at 90 h) being achieved with DO of 30%. In this test, the highest values for volumetric productivity and specific yield factor were determined. Under the different pH conditions tested, the production of laccases is favoured at DO concentration of 30% of saturation, while superior DO levels favours fungal growth. (4) Conclusion: The results indicate that dissolved oxygen concentration is a critical factor for the culture of P. sajor-caju PS-2001 and has important effects not only on laccases production but also on fungal growth.
Descritores: Oxigênio Dissolvido
Biomassa
Reatores Biológicos
Pleurotus/crescimento & desenvolvimento
Pleurotus/enzimologia
Lacase/biossíntese
Responsável: BR1.1 - BIREME


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Id: biblio-1132269
Autor: Paulova, Leona; Chmelik, Jan; Branska, Barbora; Patakova, Petra; Drahokoupil, Marek; Melzoch, Karel.
Título: Comparison of Lactic Acid Production by L. casei in Batch, Fed-batch and Continuous Cultivation, Testing the use of Feather Hydrolysate as a Complex Nitrogen Source
Fonte: Braz. arch. biol. technol;63:e20190151, 2020. tab, graf.
Idioma: en.
Projeto: MINISTRY OF EDUCATION, YOUTH AND SPORT OF THE CZECH REPUBLIC.
Resumo: Abstract A comprehensive comparison of the main fermentation parameters, productivity, yield and final L-lactic acid concentration, obtained through batch, fed-batch and continuous cultivations using Lactobacillus casei CCDM 198 and a model cultivation medium was carried out. Using this data, a pulse-feed fed-batch process was established for testing chicken feather hydrolysate as a replacement for all complex nitrogen sources (yeast and beef extracts and peptone) in the medium. As comparably high values of productivity (about 4.0 g/L/h) and yield (about 98 %) were reached under all cultivation conditions, the maximum final L-lactic acid concentration (116.5 g/L), as achieved through pulse-feed fed-batch fermentation, was chosen as the most important criterion for process selection. Fed-batch cultivation with chicken feather hydrolysate as both a complex nitrogen source and a neutralizing agent for maintaining constant culture pH yielded half the concentration of L-lactic acid compared to the model medium. We demonstrate here that chicken feather hydrolysate has potential for use in the production of L-lactic acid but its utilization requires further optimization
Descritores: Ácido Láctico/metabolismo
Fermentação
Lactobacillus casei/crescimento & desenvolvimento
-Biotecnologia/métodos
Cromatografia Líquida de Alta Pressão
Biomassa
Reatores Biológicos
Hidrólise
Limites: Animais
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME



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