Base de dados : LILACS
Pesquisa : G01.060.350.369.300 [Categoria DeCS]
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Id: biblio-1087247
Autor: Zhang, Qing; Jin, Yan-Ling; Fang, Yang; Zhao, Hai.
Título: Adaptive evolution and selection of stress-resistant Saccharomyces cerevisiae for very high-gravity bioethanol fermentation
Fonte: Electron. j. biotechnol;41:88-94, sept. 2019. tab, ilus, graf.
Idioma: en.
Projeto: Transformation of Scientific and Technological Achievements Programs of Sichuan Province; . Research Foundation of Health Management Development Center of Xihua University; . Foundation of Education Department of Sichuan Province; . Science and Technology Poverty Alleviation Programs of Sichuan Province.
Resumo: Background: In industrial yeasts, selection and breeding for resistance to multiple stresses is a focus of current research. The objective of this study was to investigate the tolerance to multiple stresses of Saccharomyces cerevisiae obtained through an adaptive laboratory evolution strategy involving a repeated liquid nitrogen freeze­thaw process coupled with multi-stress shock selection. We also assessed the related resistance mechanisms and very high-gravity (VHG) bioethanol production of this strain. Results: Elite S. cerevisiae strain YF10-5, exhibiting improved VHG fermentation capacity and stress resistance to osmotic pressure and ethanol, was isolated following ten consecutive rounds of liquid nitrogen freeze­thaw treatment followed by plate screening under osmotic and ethanol stress. The ethanol yield of YF10-5 was 16% higher than that of the parent strain during 35% (w/v) glucose fermentation. Furthermore, there was upregulation of three genes (HSP26, HSP30, and HSP104) encoding heat-shock proteins involved in the stress response, one gene (TPS1) involved in the synthesis of trehalose, and three genes (ADH1, HXK1, and PFK1) involved in ethanol metabolism and intracellular trehalose accumulation in YF10-5 yeast cells, indicating increased stress tolerance and fermentative capacity. YF10-5 also showed excellent fermentation performance during the simultaneous saccharification and fermentation of VHG sweet potato mash, producing 13.40% (w/ v) ethanol, which corresponded to 93.95% of the theoretical ethanol yield. Conclusions: A multiple-stress-tolerant yeast clone was obtained using adaptive evolution by a freeze­thaw method coupled with stress shock selection. The selected robust yeast strain exhibits potential for bioethanol production through VHG fermentation.
Descritores: Saccharomyces cerevisiae/fisiologia
Etanol/síntese química
-Saccharomyces cerevisiae/genética
Seleção Genética
Estresse Fisiológico
Trealose
Leveduras
Cruzamento
Adaptação Fisiológica
Hipergravidade
Fermentação
Reação em Cadeia da Polimerase em Tempo Real
Congelamento
Proteínas de Choque Térmico
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: biblio-889142
Autor: Arshad, Muhammad; Hussain, Tariq; Iqbal, Munawar; Abbas, Mazhar.
Título: Enhanced ethanol production at commercial scale from molasses using high gravity technology by mutant S. cerevisiae
Fonte: Braz. j. microbiol;48(3):403-409, July-Sept. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Very high gravity (VHG) technology was employed on industrial scale to produce ethanol from molasses (fermented) as well as by-products formation estimation. The effect of different Brix° (32, 36 and 40) air-flow rates (0.00, 0.20, 0.40, and 0.60 vvm) was studied on ethanol production. The maximum ethanol production was recorded to be 12.2% (v/v) at 40 Brix° with 0.2 vvm air-flow rate. At optimum level aeration and 40 Brix° VHG, the residual sugar level was recorded in the range of 12.5-18.5 g/L, whereas the viable cell count remained constant up to 50 h of fermentation and dry matter production increased with fermentation time. Both water and steam consumption reduced significantly under optimum conditions of Brix° and aeration rate with compromising the ethanol production. Results revealed VHG with continuous air flow is viable technique to reduce the ethanol production cost form molasses at commercial scale.
Descritores: Etanol/metabolismo
Microbiologia Industrial/métodos
Melaço/microbiologia
Saccharomyces cerevisiae/metabolismo
-Fermentação
Hipergravidade
Microbiologia Industrial/instrumentação
Melaço/análise
Melaço/economia
Saccharomyces cerevisiae/genética
Limites: Embrião de Galinha
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
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Id: lil-723907
Autor: Kim, H.S.; Jung, Y.Y.; Do, S.I..
Título: Hepatic inducible nitric oxide synthase expression increases upon exposure to hypergravity
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(11):940-946, 11/2014. graf.
Idioma: en.
Resumo: Stimulation by a number of conditions, including infection, cytokines, mechanical injury, and hypoxia, can upregulate inducible nitric oxide synthase (iNOS) in hepatocytes. We observed that exposure to hypergravity significantly upregulated the transcription of the hepatic iNOS gene. The aim of this study was to confirm our preliminary data, and to further investigate the distribution of the iNOS protein in the livers of mice exposed to hypergravity. ICR mice were exposed to +3 Gz for 1 h. We investigated the time course of change in the iNOS expression. Hepatic iNOS mRNA expression progressively increased in centrifuged mice from 0 to 12 h, and then decreased rapidly by 18 h. iNOS mRNA levels in the livers of centrifuged mice was significantly higher at 3, 6, and 12 h than in uncentrifuged control mice. The pattern of iNOS protein expression paralleled that of the mRNA expression. At 0 and 1 h, weak cytoplasmic iNOS immunoreactivity was found in some hepatocytes surrounding terminal hepatic venules. It was noted that at 6 h there was an increase in the number of perivenular hepatocytes with moderate to strong cytoplasmic immunoreactivity. The number of iNOS-positive hepatocytes was maximally increased at 12 h. The majority of positively stained cells showed a strong intensity of iNOS expression. The expression levels of iNOS mRNA and protein were significantly increased in the livers of mice exposed to hypergravity. These results suggest that exposure to hypergravity significantly upregulates iNOS at both transcriptional and translational levels.
Descritores: Expressão Gênica/fisiologia
Hipergravidade
Fígado/enzimologia
Óxido Nítrico Sintase Tipo II/metabolismo
RNA Mensageiro/metabolismo
-Ensaio de Imunoadsorção Enzimática
Hipergravidade/efeitos adversos
Imuno-Histoquímica
Mediadores da Inflamação/metabolismo
Interferon gama/análise
Interleucina-1beta/análise
/análise
INTERLEUKIN-ABDOMEN, ACUTE/análise
Fígado/anatomia & histologia
Fígado/fisiologia
Camundongos Endogâmicos ICR
Óxido Nítrico Sintase Tipo II/genética
Biossíntese de Proteínas/fisiologia
Reação em Cadeia da Polimerase em Tempo Real
Transcrição Genética/fisiologia
Fator de Necrose Tumoral alfa/análise
Regulação para Cima/fisiologia
Limites: Animais
Responsável: BR1.1 - BIREME


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Texto completo SciELO Chile
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Id: lil-640520
Autor: Sridee, Worawut; Laopaiboon, Lakkana; Jaisil, Prasit; Laopaiboon, Pattana.
Título: The use of dried spent yeast as a low-cost nitrogen supplement in ethanol fermentation from sweet sorghum juice under very high gravity conditions
Fonte: Electron. j. biotechnol;14(6):3-3, Nov. 2011. ilus, tab.
Idioma: en.
Projeto: National Research Council of Thailand.
Resumo: Dried spent yeast (DSY) was used as a low-cost nitrogen supplement for ethanol fermentation from sweet sorghum juice under very high gravity (VHG) conditions by Saccharomyces cerevisiae NP 01. The fermentation was carried out at 30ºC in a 5-litre bioreactor. The results showed that DSY promoted ethanol production efficiencies. The ethanol concentration (P), productivity (Qp) and yield (Yp/s) of the sterile juice (total sugar of 280 g l-1) supplemented with 8 g l-1 of DSY were not different from those supplemented with yeast extract and/or peptone at the same amount. The initial yeast cell concentration of 5 x 10(7) cells ml-1 was found to be optimal for scale-up ethanol production. In addition, an increase in sugar concentration in inoculum preparation medium (from 10 to 100 g l-1) improved the ability of the inoculum to produce ethanol under the VHG conditions. When S. cerevisiae NP 01 grown in the juice containing 100 g l-1 of total sugar was used as the inoculum for ethanol fermentation, the P, Qp and Yp/s obtained were 108.98 +/- 1.16 g l-1, 2.27 +/- 0.06 g l-1 h-1 and 0.47 +/- 0.01 g g-1, respectively. Similar results were also observed when the ethanol fermentation was scaled up to a 50-litre bioreactor under the same conditions. The cost of the sweet sorghum for ethanol production was US$ 0.63 per litre of ethanol. These results clearly indicate the high potential of using sweet sorghum juice supplemented with DSY under VHG fermentation for ethanol production in industrial applications.
Descritores: Etanol/metabolismo
Fermentação
Hipergravidade
Nitrogênio
Saccharomyces cerevisiae/fisiologia
Sorghum/metabolismo
-Leveduras
Responsável: CL1.1 - Biblioteca Central



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