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  1 / 19 LILACS  
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Id: biblio-1052039
Autor: Li, Yanjun; Zhang, Dezhi; Cai, Ningyun; Han, Chao; Mao, Qiang; Wang, Ting; Zhou, Qian; Chen, Ning; Xie, Xixian.
Título: Betaine supplementation improved L-threonine fermentation of Escherichia coli THRD by upregulating zwf (glucose-6-phosphate dehydrogenase) expression
Fonte: Electron. j. biotechnol;39:67-73, may. 2019. graf, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . National High Technology Research and Development Program; . China Postdoctoral Science Foundation.
Resumo: BACKGROUND: The supplementation of betaine, an osmoprotective compatible solute, in the cultivation media has been widely used to protect bacterial cells. To explore the effects of betaine addition on industrial fermentation, Escherichia coli THRD, an L-threonine producer, was used to examine the production of L-threonine with betaine supplementation and the underlying mechanism through which betaine functions was investigated. RESULTS: Betaine supplementation in the medium of E. coli THRD significantly improved L-threonine fermentation parameters. The transcription of zwf and corresponding enzyme activity of glucose-6-phosphate dehydrogenase were significantly promoted by betaine addition, which contributed to an enhanced expression of zwf that provided more nicotinamide adenine dinucleotide phosphate (NADPH) for L-threonine synthesis. In addition, as a result of the betaine addition, the betaine-stimulated expression of enhanced green fluorescent protein (eGFP) under the zwf promoter within a plasmid-based cassette proved to be a transcription-level response of zwf. Finally, the promoter of the phosphoenolpyruvate carboxylase gene ppc in THRD was replaced with that of zwf, while L-threonine fermentation of the new strain was promoted by betaine addition. Conclusions: We reveal a novel mode of betaine that facilitates the microbial production of useful compounds. Betaine supplementation upregulates the expression of zwf and increases the NADPH synthesis, which may be beneficial for the cell growth and thereby promote the production of L-threonine. This finding might be useful for the production of NADPH-dependent amino acids and derivatives in E. coli THRD or other E. coli strains.
Descritores: Treonina/metabolismo
Betaína/metabolismo
Escherichia coli/metabolismo
-Osmose
Via de Pentose Fosfato
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Escherichia coli/enzimologia
Fermentação
Glucosefosfato Desidrogenase/metabolismo
NADP
Responsável: CL1.1 - Biblioteca Central


  2 / 19 LILACS  
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Visintin, José Antônio
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Id: biblio-1122181
Autor: Rocha, Nathalia Alcântara; Schade, Gabriel Marra; Alves, Álvaro de Miranda; Silva, Claudia de Souza; Nakirimoto, Jacqueline Megumi; Lauri, Liura Sanchez; Campos, Lucca Gobatto; Santos, Marcelo Galvão dos; Mesquita, Nicole Nascimento; Relvas, Rachel Sordi; Carnevale, Rafaella Fernandes; Oh, Se Yoon; Gaia, Victoria Portela Diniz; Kida, Vivian Renata; Celeghini, Eneiva Carla Carvalho; Assumpção, Mayra Elena Ortiz D' Avila; Senhorini, José Augusto; Siqueira, Hatus de Oliveira; Nascimento, Nivaldo Ferreira; Silva, Luciano Andrade; Lázaro, Talita Maria; Visintin, José Antonio; Monzani, Paulo Sérgio; Yasui, George Shigueki.
Título: Acute exposure to hyperosmotic conditions reduces sperm activation by urine in the yellowtail tetra Astyanax altiparanae, a freshwater teleost fish / Exposição aguda a condições hipereosmoticas reduz ativação do sêmen por urina em lambari do-rabo-amarelo (Astyanax altiparanae), um teleósteo de água doce
Fonte: Braz. J. Vet. Res. Anim. Sci. (Online);57(3):e166205, 2020. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation (FAPESP). Young Investigators Award; Young Researcher Scholarship.
Resumo: In freshwater fish with external fertilization, sperm sampling can be contaminated with urine, which triggers motility and gives rise to decreased fertilization success. The maintenance of freshwater fish in hyperosmotic conditions may reduce urine production and improve sperm quality. Thus, the aim of this work was to verify if acute exposure to various NaCl concentrations improves sperm quality in the yellowtail tetra Astyanax altiparanae. Spermiation was induced using a single dose of carp pituitary gland (5 mg kg-1) and the males were maintained at various NaCl concentrations: NaCl 0.00% (control), NaCl 0.45% (hypoosmotic), NaCl 0.9% (isosmotic) and NaCl 1.0% (hyperosmotic) for 6 h at 26 °C. Sperm was collected and verified for activation by urine and motility traits. At 0.00%, 0.45%, and 0.90%, the sperm was motile just after sampling, indicating activation by urine. Surprisingly, at hyperosmotic conditions, no activation was observed. Other sperm and motility parameters did not show any statistical differences, including sperm viability (P = 0.7083), concentration (P = 0.9030), total motility (P = 0.6149), VCL (curvilinear velocity; P = 0.1216), VAP (average path velocity; P = 0.1231) and VSL (straight-line velocity; P = 0.1340). Our results indicate that acute maintenance at hyperosmotic conditions eliminates sperm activation by urine and maintains sperm quality. Such a new procedure is interesting for both basic and applied sciences, including reproductive practice in fish.(AU)

Em peixes de água doce com fertilização externa, a amostragem de espermatozoides pode ser contaminada pela urina, o que desencadeia motilidade e gera menor sucesso na fertilização. A manutenção de peixes de água doce em condições hiperosmóticas pode reduzir a produção de urina e melhorar a qualidade do esperma. Assim, o presente trabalho foi delineado para verificar se a exposição aguda a várias concentrações de NaCl melhora a qualidade do esperma no tetra-amarelo Astyanax altiparanae. A espermiação foi induzida usando uma dose única de hipófise da carpa (5 mg kg-1) e os machos foram mantidos em várias concentrações de NaCl: NaCl 0,00% (controle), NaCl 0,45% (hipoosmótico), NaCl 0,9% (isosmótico) e NaCl 1,0% (hiperosmótico) por seis horas a 26 °C. O esperma foi colhido e verificado quanto à ativação por urina e traços de motilidade. Em 0,00%, 0,45%, 0,90% os espermatozóides eram móveis logo após a amostragem, indicando ativação pela urina. Surpreendentemente, em condições hiperosmóticas, nenhuma ativação foi observada. Outros parâmetros espermáticos e de motilidade não mostraram diferenças estatísticas, incluindo viabilidade espermática (P = 0,7083), concentração (P = 0,9030), motilidade total (P = 0,6149), VCL (Velocidade Curvilinear; P = 0,1216), VMD (Velocidade Média de Deslocamento; P = 0,1230) e VLR (Velocidade em linha Reta; P = 0,1340). Nossos resultados indicam que a manutenção aguda em condições hiperosmóticas elimina a ativação do esperma pela urina e mantém a qualidade do esperma. Esse novo procedimento é interessante para as ciências básicas e aplicadas, incluindo a prática reprodutiva em peixes.(AU)
Descritores: Osmose
Salinidade
Análise do Sêmen/métodos
Análise do Sêmen/veterinária
Characidae/fisiologia
-Motilidade Espermática
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


  3 / 19 LILACS  
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Id: lil-774031
Autor: Cisneros, Ligia de Loiola; Salgado, Audrey Heloisa Ivanenko.
Título: Guia de eletroterapia: princípios biofísicos, conceitos e aplicações clínicas / Electrotherapy Guide: biophysical principles, concepts and clinical applications.
Fonte: Belo Horizonte; Coopmed; 2006. 96 p. ilus, tab, graf.
Idioma: pt.
Descritores: Terapia por Estimulação Elétrica
Iontoforese
Osmose
Manejo da Dor
Estimulação Elétrica Nervosa Transcutânea
Limites: Humanos
Responsável: BR75.1 - Biblioteca


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Id: lil-763714
Autor: Cisternas C., Daniel.
Título: Cuando la constipación no responde a fibra: ¿qué más hacer? / When constipation does not respond to dietary fiber: what else can we do?
Fonte: Gastroenterol. latinoam;24(supl.1):S20-S24, 2013. ilus, tab.
Idioma: es.
Resumo: Constipation should be defined by symptoms, and is refractory to fiber in about 40 percent of the cases. About 50 percent of these cases caused by a defecatory disorder, usually dyssynergic defecation, which is defined as inadequate relaxation of the pelvic floor during straining. In this cases, biofeedback therapy produces an adequate relief in about 80 percent. It is suggested that ano-rectal manometry with balloon expulsion test should be the first approach in cases of fiber-refractory constipation. In cases of normal ano-rectal test, an evaluation of colonic transit time is suggested. In cases of slow transit constipation, treatment responses are much lower. Osmotic laxatives, especially polyethilenglicol, are recommended in all types of constipation. There are new laxatives, like lubiprostone and linaclotide, and new prokinetics, like prucalopride, that are already available in different countries, that show a reasonable good response profile.

La constipación debe ser definida por síntomas, y es refractaria al uso de fibra en alrededor de 4 por ciento de los casos. En alrededor de 50 por ciento existe un defecto expulsivo, casi siempre defecación disinérgica, la que es definida por una relajación inadecuada del piso pelviano al momento de pujar. Se recomienda un estudio de manometría ano-rectal con test de expulsión del balón como primera opción en estos casos. En los casos de disinergia, la terapia con biofeedback tiene una eficacia del alrededor de 80 por ciento, beneficio que se mantiene a largo plazo. En casos de manometría ano-rectal normal, se sugiere evaluar un tiempo de transito colónico, pues la respuesta de los casos con tránsito lento es bastante menor. En todos los casos de constipación se recomienda el uso de laxantes osmóticos tipo polietilenglicol. Existen nuevos laxantes, como lubiprostona y linaclotide, y nuevos procinéticos como prucalopride, que ya están disponibles en diversos países del mundo, que tienen una eficacia razonable en estos casos.
Descritores: Constipação Intestinal/terapia
Retroalimentação Fisiológica
Laxantes/uso terapêutico
Polietilenoglicóis/uso terapêutico
-Constipação Intestinal/diagnóstico
Constipação Intestinal/fisiopatologia
Manometria/métodos
Osmose
Limites: Humanos
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


  5 / 19 LILACS  
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Texto completo SciELO Venezuela
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Id: lil-714890
Autor: Valero, Yolmar; Colina, Jhoana; Ineichen, Emilio.
Título: Efecto del procesamiento sobre la capacidad antioxidante de la ciruela criolla (Prunus domestica)
Fonte: Arch. latinoam. nutr;62(4):363-369, dic. 2012. ilus, tab.
Idioma: es.
Resumo: Las frutas son consideradas fuentes de compuestos antioxidantes, cuyas propiedades pudieran desmejorar debido al procesamiento. El objetivo de esta investigación fue determinar el efecto del escaldado y la deshidratación osmótica sobre el contenido de polifenoles totales, taninos y la capacidad antioxidante de la ciruela criolla (Prunus domestica) en sus variedades amarilla y roja. La concentración de los polifenoles totales se determinó mediante el método Folin-Ciocalteu, los taninos por el método de la vanilina y la capacidad antioxidante por la eficiencia antirradical (EA) y el método de poder reductor férrico (FRP). El contenido de polifenoles totales y los taninos fueron mayores en la ciruela roja que en la amarilla. En las dos variedades, el mayor contenido de polifenoles se encontró en la pulpa, mientras que los taninos se encontraron en mayor proporción en las cáscaras. La ciruela roja presentó mayor capacidad antioxidante, siendo la EA baja y de cinética lenta para las dos variedades. Se observó una correlación lineal entre los polifenoles y los taninos con la eficiencia antirradical, sin embargo, con el poder reductor no hubo correlación. El escaldado incrementó el contenido de polifenoles, mientras que los taninos y la EA disminuyeron, el poder reductor no se vio afectado por los tratamientos. Para la deshidratación osmótica se obtuvo una disminución significativa de los taninos y la eficiencia antirradical, mientras que los polifenoles y el poder reductor no se vieron afectados por el procesamiento. Se recomienda el escaldado como alternativa de consumo y conservación en la ciruela criolla.

Effect of processing on the antioxidant capacity of the plum (Prunus domestica). Fruits are considered sources of antioxidant compounds whose properties could impair due to processing. The objective of this work was to determine the effect of blanching and osmotic dehydration on the total polyphenols content, tannins and antioxidant capacity of plums (Prunus domestica) in yellow and red varieties. The total phenolic content in plums was determined according to the Folin-Ciocalteu assay and tannins were determined by vanillin assay. The antiradical efficiency (AE) and ferric reducing power (FRP) were used to estimate the total antioxidant capacity. The content of total polyphenols and tannins were higher in the red plum. The content of polyphenols in the pulp was higher that the peel while for tannins the opposite was observed in both varieties. The red plum had higher antioxidant capacity. The AE was low and slow kinetics for the two varieties. There was a linear correlation between polyphenols and tannins with antiradical efficiency; however, there was no correlation with the reducing power. The total polyphenols content was increased with blanching, while the tannins and the AE decreased, ferric reducing power is unaffected. For osmotic dehydration, the tannins and the AE were decreased, while the total polyphenols content and ferric reducing power are unaffected.It is recommended the blanched as an alternative to consumption and conservation in the plum.
Descritores: Antioxidantes/análise
Manipulação de Alimentos/métodos
Frutas/química
Fenóis/análise
Prunus/química
Taninos/análise
-Dessecação/métodos
Osmose
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


  6 / 19 LILACS  
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Texto completo SciELO Chile
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Id: lil-602980
Autor: Saeed, Muhammad; Guo, Wangzhen; Ullah, Ihsan; Tabbasam, Nabila; Zafar, Yusuf; Zhang, Tianzhen; Rahman, Mehboob ur.
Título: QTL mapping for physiology, yield and plant architecture traits in cotton (Gossypium hirsutum L. ) grown under well-watered versus water-stress conditions
Fonte: Electron. j. biotechnol;14(3):3-3, May 2011. ilus, tab.
Idioma: en.
Projeto: High-tech program 863; . Jiangsu province key project; . 111 Project; . China and Pakistan Joint Research and Development Project.
Resumo: Increasing scarcity of irrigation water is a major threat to sustainable production of cotton (Gossypium hirsutum L.). Identifying genomic regions contributing to abiotic stress tolerance will help develop cotton cultivars suitable for water-limited regions through molecular marker-assisted breeding. A molecular mapping F2 population was derived from an intraspecific cross of the drought sensitive G. hirsutum cv. FH-901 and drought tolerant G. hirsutum cv. RH-510. Field data were recorded on physiological traits (osmotic potential and osmotic adjustment); yield and its component traits (seedcotton yield, number of bolls/plant and boll weight); and plant architecture traits (plant height and number of nodes per plant) for F2, F2:3 and F2:4 generations under well-watered versus water-limited growth conditions. The two parents were surveyed for polymorphism using 6500 SSR primer pairs. Joinmap3.0 software was used to construct linkage map with 64 polymorphic markers and it resulted into 35 markers mapped on 12 linkage groups. QTL analysis was performed by composite interval mapping (CIM) using QTL Cartographer2.5 software. In total, 7 QTLs (osmotic potential 2, osmotic adjustment 1, seedcotton yield 1, number of bolls/plant 1, boll weight 1 and plant height 1) were identified. There were three QTLs (qtlOP-2, qtlOA-1, and qtlPH-1) detected only in water-limited conditions. Two QTLs (qtlSC-1 and qtlBW-1) were detected for relative values. Two QTLs (qtlOP-1 and qtlBN-1) were detected for well-watered treatment. Significant QTLs detected in this study can be employed in MAS for molecular breeding programs aiming at developing drought tolerant cotton cultivars.
Descritores: Secas
Gossypium/fisiologia
Gossypium/genética
Locos de Características Quantitativas
-Adaptação Fisiológica
DNA de Plantas/genética
Variação Genética
Gossypium/crescimento & desenvolvimento
Mapeamento Cromossômico/métodos
Osmose
Polimorfismo Genético
Software
Responsável: CL1.1 - Biblioteca Central


  7 / 19 LILACS  
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Texto completo SciELO Brasil
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Id: lil-601833
Autor: Basso, Gabriela Romanini; Della Bona, Álvaro; Gobbi, Delton Luiz; Cecchetti, Dileta.
Título: Fluoride Release from Restorative Materials
Fonte: Braz. dent. j;22(5):355-358, 2011. tab.
Idioma: en.
Resumo: The aim of this study was to evaluate in vitro fluoride (F) release from 4 restorative materials (3M ESPE): Ketak Molar Easymix [KME - conventional glass ionomer cement (GIC)]; Rely-X luting 2 [RL2 - resin-modified GIC (RMGIC)]; Vitremer (VIT- RMGIC); and Filtek Z250 [Z250 - negative control]. Disc-shaped specimens were fabricated according to the manufacturer’s instructions and placed into 10 mL of reverse osmosis water at 37°C until the analyses were done using a liquid membrane for selective F ion electrode (Orion 710). F release was evaluated every 6 h in the first day and thereafter daily during 28 days (d). The results were analyzed statistically by two-way ANOVA and Tukey’s test (α=0.05). Mean F release and standard deviation values (in ppm) were: KME: 6 h- 0.34 ± 0.04; 24 h- 1.22 ± 0.30; 7 d- 0.29 ± 0.09; 14 d- 0.20 ± 0.04; 28 d- 0.16 ± 0.01; RL2: 6 h- 2.46 ± 0.48; 24 h-12.33 ± 2.93; 7 d- 1.37 ± 0.38; 14 d- 0.80 ± 0.13; 28 d- 0.80 ± 0.21; VIT: 6 h- 0.98 ± 0.35; 24 h- 4.35 ± 1.22; 7 d- 0.66 ± 0.23; 14 d- 0.40 ± 0.07; 28 d- 0.39 ± 0.08; Z250: 6 h- 0.029 ± 0.001; 24 h- 0.024 ± 0.009; 7 d- 0.023 ± 0.004; 14 d- 0.025 ± 0.001; 28 d- 0.028 ± 0.001. RL2 RMGIC released more F than the other materials in all periods. The greatest release of F occurred in the first 24 h.

O objetivo deste estudo foi avaliar, in vitro, a liberação de flúor (F) de 4 materiais restauradores (3M-ESPE): KME cimento de ionômero de vidro convencional (CIV) (Ketak Molar Easymix); RX2- GIC modificado por resina (RMGIC) (Rely-X luting 2); VIT-RMGIC (Vitremer); -resina composta Z250 (controle negativo) (Filtek Z250). As amostras foram confeccionadas de acordo com as instruções do fabricante e colocadas em 10 mL de água destilada 37°C até as leituras, que foram feitas utilizando um eletrodo de íon seletivo de fluoreto de membrana líquida (Orion 710). A liberação de flúor foi avaliada a cada 6 h no primeiro dia e, diariamente durante 28 dias (d). Os resultados foram analisados estatisticamente usando ANOVA a dois critérios e teste de Tukey. A média e desvio padrão de liberação de F, valores (em ppm) foram: KME: 6 h- 0,34 ± 0,04; 24 h- 1,22 ± 0,30; 7 d- 0,29 ± 0,09; 14 d- 0,20 ± 0,04; 28 d- 0,16 ± 0,01; RL2: 6 h- 2,46 ± 0,48; 24 h- 12,33 ± 2,93; 7 d- 1,37 ± 0,38; 14 d- 0,80 ± 0,13; 28 d- 0,80 ± 0,21; VIT: 6 h- 0,98 ± 0,35; 24 h- 4,35 ± 1,22; 7 d- 0,66 ± 0,23; 14 d- 0,40 ± 0,07; 28 d- 0,39 ± 0,08; Z250: 6 h- 0,029 ± 0,001; 24 h- 0,024 ± 0,009; 7 d- 0,023 ± 0,004; 14 d- 0,025 ± 0,001; 28 d- 0,028 ± 0,001. Concluiu-se que RL2 liberou mais F do que os outros materiais em todos os períodos. A maior liberação de F ocorreu nas primeiras 24 h.
Descritores: Cariostáticos/química
Materiais Dentários/química
Fluoretos/química
-Resinas Compostas/química
Difusão
Cimentos de Ionômeros de Vidro/química
Eletrodos Íon-Seletivos
Teste de Materiais
Osmose
Potenciometria/instrumentação
Cimentos de Resina/química
Temperatura
Fatores de Tempo
Água/química
Limites: Humanos
Responsável: BR1.1 - BIREME


  8 / 19 LILACS  
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Id: lil-557507
Autor: Ayala A., Alfredo A; Serna C., Liliana; Mosquera V., Esmeralda S.
Título: Liofilización de pitahaya amarilla (Selenicereus megalanthus) / Freeze-drying in yellow pitahaya (Selenicereus megalanthus)
Fonte: Vitae (Medellín);17(2):121-127, mayo-ago. 2010.
Idioma: es.
Resumo: La liofilización es una alternativa de interés como método de conservación de alimentos, la cual permite prolongar el tiempo de vida útil manteniendo significativamente las propiedades físicas y fisicoquímicas relacionadas con su calidad. El objetivo de este trabajo fue evaluar el efecto de la liofilización y la osmoliofilización sobre las cinéticas de congelación y de secado, la actividad de agua, el volumen, la porosidad y la capacidad de rehidratación en rodajas de pitahaya amarilla (Selenicereus megalanthus). Como pretratamiento osmótico a la liofilización (osmoliofilización), las rodajas se sumergieron en una solución de sacarosa con 55 ºBrix a 25ºC; se congelaron a -35°C, la sublimación se llevó a presión de vacío de 8 Pa y el secado se realizó desde -35 hasta 35°C. Los resultados de los tratamientos evidenciaron, al final del proceso, diferencias significativas en la porosidad, volumen y capacidad de rehidratación (que fueron mayores para el tratamiento liofilizado), mientras que la cinética de secado y la actividad de agua no presentaron diferencias significativas. El tratamiento de liofilización fue adecuado para la conservación de rodajas de pitahaya amarilla, ya que permitió reducir la actividad de agua por debajo de 0,4; conservó significativamente el volumen y presentó alta capacidad de rehidratación.

Freeze-drying is an interesting alternative as a food preservation method allowing to extend the shelf life and significantly preserving physical and physicochemical properties related to quality. The objective of this study was to evaluate the effect of freeze-drying and osmo-freeze-drying on the kinetics of freezing and drying, water activity, volume, porosity and rehydration capacity of sliced yellow pitahaya (Selenicereus megalanthus). As osmotic pretreatment to freeze-drying (osmo-freeze-drying), slices were immersed in a sucrose solution at 55°Brix. The slices were frozen at -35°C. The sublimation was carried at vacuum pressure of 8 Pa and drying was carried out from -35 to 35°C. The results of these treatments at the end of the process showed significant differences in porosity, volume and rehydration capacity (being higher for freeze-drying treatment), while the kinetics of drying and water activity were not significant. The freeze drying treatment was suitable for the preservation of sliced yellow pitahaya, because it helped to decrease water activity below 0.4, preserved and showed significant volumes of high rehydration capacity.
Descritores: Cactaceae
Desidratação
Conservação de Alimentos
Liofilização
Osmose
Responsável: CO56.3 - Biblioteca


  9 / 19 LILACS  
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Texto completo SciELO Venezuela
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Id: lil-473605
Autor: Simpson R., Ricardo; Jiménez P., Maite; Carevic G., Erica; Grancelli M., Romina.
Título: Aceleración de la deshidratación osmótica de frambuesas (Rubus idaeus) por medio de calentamiento óhmico / Acceleration of osmotic dehydration process through ohmic heating of foods: raspberries (Rubus idaeus)
Fonte: Arch. latinoam. nutr;57(2):192-195, jun. 2007. ilus, tab, graf.
Idioma: es.
Resumo: Frambuesas (Rubus idaeus) se deshidrataron osmóticamente a través de un tratamiento convencional bajo el supuesto de solución homogénea, utilizando como medio una solución de glucosa al 62% a una temperatura de 50ºC. También se deshidrataron osmóticamente por medio de calentamiento óhmico, utilizando como medio una solución de glucosa al 57%, con voltaje variable (para mantener una temperatura entre 40-50ºC) y unaintensidad del campo eléctrico <100 V/cm. Al comparar los resultados se observa una evidente disminución en el tiempo de proceso al utilizar el calentamiento óhmico. En algunos casos, ésta reducción alcanzó hasta un 50%. Esto se explica por el efecto adicional al daño térmico que se genera en un proceso óhmico, denominado electroporación.

Acceleration of osmotic dehydration process through ohmic heating of foods: raspberries (Rubus idaeus). Raspberries (Rubus idaeus) were osmotically dehydrated by applying a conventional method under the supposition of a homogeneous solution, all in a 62% glucose solution at 50ºC. Raspberries (Rubus idaeus) were also osmotically dehydrated by using ohmic heating in a 57% glucose solution at a variable voltage (to maintain temperaturebetween 40 and 50ºC) and an electric field intensity <100 V/cm. When comparing the results from both experiments it was evident that processing time is reduced when ohmic heating technique wasused. In some cases this reduction reached even 50%. This is explained by the additional effect to the thermal damage that is generated in an ohmic process, denominated electroporation.
Descritores: Dessecação/métodos
Frutas
Manipulação de Alimentos/métodos
Temperatura Alta
Rosaceae
-Osmose
Fatores de Tempo
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


  10 / 19 LILACS  
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Id: lil-445290
Autor: Fernandes, L; Araújo, M. A; Amaral, A; Reis, V. C; Martins, N. F; Felipe, M. S.
Título: Cell signaling pathways in Paracoccidioides brasiliensis: inferred from comparisons with other fungi
Fonte: Genet. mol. res. (Online);4(2):216-231, 30 jun. 2005. ilus, tab.
Idioma: en.
Resumo: The human fungal pathogen Paracoccidioides brasiliensis is an ascomycete that displays a temperature-dependent dimorphic transition, appearing as a mycelium at 22 degrees C and as a yeast at 37 degrees C, this latter being the virulent form. We report on the in silico search made of the P. brasiliensis transcriptome-expressed sequence tag database for components of signaling pathways previously known to be involved in morphogenesis and virulence in other species of fungi, including Saccharomyces cerevisiae, Cryptococcus neoformans, Candida albicans, and Aspergillus fumigatus. Using this approach, it was possible to identify several protein cascades in P. brasiliensis, such as i) mitogen-activated protein kinase signaling for cell integrity, cell wall construction, pheromone/mating, and osmo-regulation, ii) the cAMP/PKA system, which regulates fungal development and virulence, iii) the Ras protein, which allows cross-talking between cascades, iv) calcium-calmodulin-calcineurin, which controls cell survival under oxidative stress, high temperature, and membrane/cell wall perturbation, and v) the target of rapamycin pathway, controlling cell growth and proliferation. The ways in which P. brasiliensis responds to the environment and modulates the expression of genes required for its survival and virulence can be inferred through comparison with other fungi for which this type of data is already available.
Descritores: Etiquetas de Sequências Expressas
Paracoccidioides/fisiologia
Proteínas Fúngicas/metabolismo
Transcrição Genética
Transdução de Sinais/genética
-Feromônios/metabolismo
Fungos/citologia
Fungos/metabolismo
Fungos/patogenicidade
Osmose/fisiologia
Paracoccidioides/metabolismo
Paracoccidioides/patogenicidade
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Proteínas Quinases Dependentes de AMP Cíclico/metabolismo
Proteínas ras/metabolismo
Alinhamento de Sequência
Transdução de Sinais/fisiologia
Limites: Humanos
Responsável: BR1.1 - BIREME



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