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Pesquisa : G01.906.595 [Categoria DeCS]
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Id: biblio-951815
Autor: Teleken, Jhony Tiago; Galvão, Alessandro Cazonatto; Robazza, Weber da Silva.
Título: Use of modified Richards model to predict isothermal and non-isothermal microbial growth
Fonte: Braz. j. microbiol;49(3):614-620, July-Sept. 2018. tab, graf.
Idioma: en.
Projeto: We would like to thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: Abstract Mathematical models are often used to predict microbial growth in food products. An important class of these models involves the adaptation of classical sigmoid functions, such as the Gompertz and logistic functions. This study aimed to validate the use of the modified Richards model in various situations, which have not previously been tested. The model was obtained through solving a system of two differential equations and could be applied to both isothermal and non-isothermal environments. To test and validate this model, we used published datasets containing data for the growth of Pseudomonas spp. in fish products. The results obtained after fitting the model showed that it could be effectively used to describe and predict the Pseudomonas growth curves under various temperature regimens. However, the influence of the shape parameter on the growth curve is an issue that needs further evaluation.
Descritores: Pseudomonas/crescimento & desenvolvimento
Cinética
-Pseudomonas/química
Temperatura Ambiente
Produtos Pesqueiros/microbiologia
Modelos Teóricos
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-974317
Autor: Zepeda, Andrea B; Pessoa Jr, Adalberto; Farías, Jorge G.
Título: Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast
Fonte: Braz. j. microbiol;49(supl.1):119-127, 2018. tab, graf.
Idioma: en.
Projeto: Comisión Nacional de Investigación Científica y Tecnológica de Chile; . AZP; . FAPESP.
Resumo: Abstract Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use methanol efficiently as the sole carbon and energy source. Pichia pastoris is a methylotrophic yeast characterized as being an economical, fast and effective system for heterologous protein expression. Many factors can affect both the product and the production, including the promoter, carbon source, pH, production volume, temperature, and many others; but to control all of them most of the time is difficult and this depends on the initial selection of each variable. Therefore, this review focuses on the selection of the best promoter in the recombination process, considering different inductors, and the temperature as a culture medium variable in methylotrophic Pichia pastoris yeast. The goal is to understand the effects associated with different factors that influence its cell metabolism and to reach the construction of an expression system that fulfills the requirements of the yeast, presenting an optimal growth and development in batch, fed-batch or continuous cultures, and at the same time improve its yield in heterologous protein production.
Descritores: Pichia/genética
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/genética
Carbono/metabolismo
Regiões Promotoras Genéticas
-Pichia/crescimento & desenvolvimento
Pichia/metabolismo
Temperatura Ambiente
Microbiologia Industrial
Responsável: BR1.1 - BIREME


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Id: biblio-974316
Autor: Reis, Cristiane Bianchi Loureiro dos; Morandini, Liziane Maria Barassuol; Bevilacqua, Caroline Borges; Bublitz, Fabricio; Ugalde, Gustavo; Mazutti, Marcio Antonio; Jacques, Rodrigo Josemar Seminoti.
Título: First report of the production of a potent biosurfactant with α, ß-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation
Fonte: Braz. j. microbiol;49(supl.1):185-192, 2018. tab, graf.
Idioma: en.
Resumo: Abstract Biosurfactants have many advantages over synthetic surfactants but have higher production costs. Identifying microorganisms with high production capacities for these molecules and optimizing their growth conditions can reduce cost. The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule. The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi. To optimize biosurfactant production, a Plackett-Burman design and a central composite rotational design were used. The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose. The partial structure of the biosurfactant molecule was identified by nuclear magnetic resonance spectrometry. F. fujikuroi reduced surface tension from 72 to 20 mN m−1 under the optimized conditions of pH 5.0, 37 °C and 7 days of incubation with 190 rpm agitation. The partial identification of the structure of the biosurfactant demonstrated the presence of an α,β-trehalose. The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.
Descritores: Tensoativos/metabolismo
Trealose/metabolismo
Microbiologia Industrial/métodos
Fusarium/metabolismo
-Tensoativos/química
Temperatura Ambiente
Meios de Cultura/metabolismo
Fermentação
Fusarium/crescimento & desenvolvimento
Fusarium/química
Concentração de Íons de Hidrogênio
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-974294
Autor: Zepeda, Andrea B; Figueroa, Carolina A; Pessoa, Adalberto; Farías, Jorge G.
Título: Free fatty acids reduce metabolic stress and favor a stable production of heterologous proteins in Pichia pastoris
Fonte: Braz. j. microbiol;49(4):856-864, Oct.-Dec. 2018. graf.
Idioma: en.
Projeto: FAPESP.
Resumo: ABSTRACT The growth of yeasts in culture media can be affected by many factors. For example, methanol can be metabolized by other pathways to produce ethanol, which acts as an inhibitor of the heterologous protein production pathway; oxygen concentration can generate aerobic or anaerobic environments and affects the fermentation rate; and temperature affects the central carbon metabolism and stress response protein folding. The main goal of this study was determine the implication of free fatty acids on the production of heterologous proteins in different culture conditions in cultures of Pichia pastoris. We evaluated cell viability using propidium iodide by flow cytometry and thiobarbituric acid reactive substances to measure cell membrane damage. The results indicate that the use of low temperatures and low methanol concentrations favors the decrease in lipid peroxidation in the transition phase from glycerol to methanol. In addition, a temperature of 14 ºC + 1%M provided the most stable viability. By contrast, the temperature of 18 ºC + 1.5%M favored the production of a higher antibody fragment concentration. In summary, these results demonstrate that the decrease in lipid peroxidation is related to an increased production of free fatty acids.
Descritores: Pichia/metabolismo
Ácidos Graxos não Esterificados/metabolismo
-Pichia/crescimento & desenvolvimento
Pichia/genética
Temperatura Ambiente
Proteínas Recombinantes/genética
Meios de Cultura/metabolismo
Meios de Cultura/química
Metanol/metabolismo
Fermentação
Glicerol/metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-1046244
Autor: Ferrero, Fernando; Abrutzky, Rosana; Ossorio, María Fabiana; Torres, Fernando.
Título: Efectos de la contaminación y el clima en las consultas pediátricas por infección respiratoria aguda en la Ciudad de Buenos Aires / Effects of contamination and climate in the Pediatric Emergency Department visits for acute respiratory infection in the City of Buenos Aires
Fonte: Arch. argent. pediatr;117(6):368-374, dic. 2019. tab.
Idioma: en; es.
Resumo: Introducción: La contaminación y el clima tienen impacto en la patología respiratoria en niños; pocos estudios lo evaluaron en la Ciudad de Buenos Aires.Objetivo: Evaluar el impacto de la interacción entre contaminantes atmosféricos y clima en las consultas por infección respiratoria aguda baja (IRAB) en un hospital pediátrico.Métodos: Estudio ecológico, de series temporales con modelos aditivos generalizados, que incluyó todas las consultas y consultas por IRAB (urgencias) entre 2012 y 2016. Para controlar sesgos, se conformó una serie con promedios móviles de 7 días para consultas por IRAB. Los predictores fueron niveles diarios de contaminantes atmosféricos (monóxido de carbono, dióxido nitroso, material particulado < 10 µ) y variables meteorológicas (temperatura, humedad). Los contaminantes fueron medidos en 3 estaciones de monitoreo. Se controló por variables temporales (día de la semana, semestre cálido/frío).Resultados: Las consultas totales fueron 455 256 y por IRAB 17 298. Solo consultas totales correlacionaron con día de la semana (lunes y sábados, más consultas y jueves, menos). El semestre cálido registró menos consultas por IRAB que el frío (riesgo relativo = 0,23; intervalo de confianza 95 %: 0,29-0,18; p < 0,001).Una estación de monitoreo no presentó ninguna correlación; las otras mostraron correlación débil entre monóxido de carbono y material particulado < 10 µ y consultas por IRAB.Conclusión: La época del año explica con precisión el incremento del número de consultas totales y por IRAB. Aunque el nivel de algunos contaminantes muestra correlación con el número de consultas, su impacto es irrelevante.Palabras clave: contaminación del aire, clima, infecciones del sistema respiratorio, niño.

Introduction: Pollution and climate have an impact on pediatric respiratory diseases; few studies have assessed this in the Autonomous City of Buenos Aires.Objective: To assess the impact of the interaction between air pollutants and climate on the Emergency Department visits for acute lower respiratory tract infection (ALRTI) in a children's hospital.Methods: Ecological, time-series study with generalized additive models that included total visits and visits for ALRTI to the Emergency Department between 2012 and 2016. A series with 7-day moving averages for ALRTI visits was founded as a bias control measure. Predictors were daily levels of air pollutants (carbon monoxide, nitrous dioxide, particulate matter < 10 µ) and meteorological variables (temperature, humidity). Pollutants were measured at three monitoring stations. Temporal variables (day of the week, warm/cold semester) were controlled.Results: There were 455 256 total visits; 17 298 accounted for visits for ALRTI. A correlation was established only between total visits and day of the week (Mondays and Saturdays, more visits; Thursdays, less visits). Less visits for ALRTI were recorded in the warm semester compared to the cold semester (relative risk = 0.23; 95 % confidence interval: 0.29-0.18; p < 0.001). One monitoring station did not show any correlation; the other two stations showed a weak correlation between carbon monoxide and particulate matter < 10 µ and visits for ALRTI. Conclusion: The season accurately accounts for the increased number of total visits and visits for ALRTI. Although there was a correlation between the level of certain pollutants and the number of visits, its impact was irrelevant.
Descritores: Vírus Sinciciais Respiratórios
Infecções Respiratórias
Temperatura Ambiente
Mudança Climática
Poluição do Ar
Serviço Hospitalar de Emergência
-Estudos de Séries Temporais
Estudos Ecológicos
Limites: Seres Humanos
Masculino
Feminino
Criança
Responsável: AR94.1 - Centro de Información Pediatrica


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Id: biblio-1049197
Autor: Ferreira, Débora Teresa da Rocha Gomes; Gonçalves, Edilma Pereira; Viana, Jeandson Silva; Ralph, Lidiana Nayara; Silva, Júlio Cesar de Almeida; Silva, Edila Maria da.
Título: Temperature and light under the physiological potential of seeds of Handroanthus impetiginosus / Temperatura e luz sob o potencial fisiologico de sementes de Handroanthus impetiginosus
Fonte: Biosci. j. (Online);36(1):68-77, jan./feb. 2020. graf.
Idioma: en.
Resumo: H. impetiginosus belongs to the Bignoniaceae family; it has a great potential for economic exploitation and can be used in landscaping of urban areas, reforestation, recovery of degraded areas, and folk medicine. The experiment was carried out to evaluate the effect of light and temperature regimes on the germination and vigor of Handroanthus impetiginosus seeds at the Seed Analysis Laboratory of UFRPE/UAG. The seeds were subjected to light regimes: white, far red, red, and no light at 15°C, 20°C, 25°C, 30°C, 35°C, and 40°C, using a completely randomized experimental design in a factorial scheme (4 × 6), with four repetitions of 25 seeds. The different light regimes did not influence the seed germination of H. impetiginosus. The highest germination percentage (92%) and germination speed index (7.94) were obtained at temperatures 28.2°C and 29.2°C, respectively, both under red light. The longest seedling length was also obtained from the seeds subjected to red light regime at 25°C. The temperatures of 15°C and 40°C inhibited the germination of H. impetiginosus seeds. H. impetiginosus seeds are classified as neutral photoblastics, and constant temperatures of 28.2°C and 29.2°C provided maximum germination.

H. impetiginosus, pertencente à família Bignoniaceae, apresenta grande potencial para exploração econômica, podendo ser utilizado no paisagismo de áreas urbanas, reflorestamentos, recuperação de áreas degradadas e na medicina popular. O experimento foi conduzido no Laboratório de Análise de Sementes da UFRPE/UAG com o objetivo de avaliar o efeito dos regimes de luz e temperatura na germinação e vigor de sementes de Handroanthus impetiginosus. As sementes foram submetidas aos regimes de luz: branca, vermelho distante, vermelho e ausência de luz sob as temperaturas de 15, 20, 25, 30, 35 e 40°C, sendo utilizado o delineamento experimental inteiramente ao acaso, em esquema fatorial (4x6), com quatro repetições de 25 sementes. Os diferentes regimes de luz não influenciaram na germinação de sementes de H.impetiginosus. A maior porcentagem de germinação (92%) e índice de velocidade de germinação (7,94) foram obtidos nas temperaturas 28,2 e 29,2°C, respectivamente, ambos no regime de luz vermelha. O maior comprimento de plântula também foi obtido de sementes submetidas ao regime de luz vermelha na temperatura de 25ºC. As temperaturas de 15ºC e 40ºC inibiram a germinação das sementes de H. impetiginosus. As sementes de H. impetiginosus são classificadas como fotoblásticas neutras e as temperaturas constantes de 28,2 e 29,2°C proporcionaram máxima germinação.
Descritores: Sementes
Temperatura Ambiente
Germinação
Tabebuia
Luz
Responsável: BR396.1 - Biblioteca Central


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Id: biblio-828208
Autor: Munaganti, Rajesh Kumar; Muvva, Vijayalakshmi; Konda, Saidulu; Naragani, Krishna; Mangamuri, Usha Kiranmayi; Dorigondla, Kumar Reddy; Akkewar, Dattatray. M.
Título: Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards
Fonte: Braz. j. microbiol;47(4):1030-1038, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract An actinobacterial strain VL-RK_09 having potential antimicrobial activities was isolated from a mango orchard in Krishna District, Andhra Pradesh (India) and was identified as Arthrobacter kerguelensis. The strain A. kerguelensis VL-RK_09 exhibited a broad spectrum of in vitro antimicrobial activity against bacteria and fungi. Production of bioactive metabolites by the strain was the highest in modified yeast extract malt extract dextrose broth, as compared to other media tested. Lactose (1%) and peptone (0.5%) were found to be the most suitable carbon and nitrogen sources, respectively, for the optimum production of the bioactive metabolites. The maximum production of the bioactive metabolites was detected in the culture medium with an initial pH of 7, in which the strain was incubated for five days at 30 °C under shaking conditions. Screening of secondary metabolites obtained from the culture broth led to the isolation of a compound active against a wide variety of Gram-positive and negative bacteria and fungi. The structure of the first active fraction was elucidated using Fourier transform infrared spectroscopy, electrospray ionization mass spectrometry, 1H and 13C nuclear magnetic resonance spectroscopy. The compound was identified as S,S-dipropyl carbonodithioate. This study is the first report of the occurrence of this compound in the genus Arthrobacter.
Descritores: Arthrobacter/isolamento & purificação
Arthrobacter/metabolismo
Mangifera/microbiologia
Anti-Infecciosos/metabolismo
Anti-Infecciosos/farmacologia
-Sais/metabolismo
Temperatura Ambiente
Carbono/metabolismo
Testes de Sensibilidade Microbiana
Metaboloma
Metabolômica/métodos
Concentração de Íons de Hidrogênio
Anti-Infecciosos/isolamento & purificação
Anti-Infecciosos/química
Minerais/metabolismo
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-828205
Autor: Aliabadi, Nasrin; Aminzadeh, Saeed; Karkhane, Ali Asghar; Haghbeen, Kamahldin.
Título: Thermostable chitinase from Cohnella sp. A01: isolation and product optimization
Fonte: Braz. j. microbiol;47(4):931-940, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Projeto: International Foundation for Science; . Committee on Scientific and Technological Cooperation; . National Institute of Genetic Engineering and Biotechnology.
Resumo: Abstract Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequencing and by matching the key morphological, physiological, and biochemical characteristics. The cultivation of Cohnella sp. A01 in the suitable liquid medium resulted in the production of high levels of enzyme. The colloidal chitin, peptone, and K2HPO4 represented the best carbon, nitrogen, and phosphorus sources, respectively. Enzyme production by Cohnella sp. A01 was optimized by the Taguchi method. Our results demonstrated that inoculation amount and temperature of incubation were the most significant factors influencing chitinase production. From the tested values, the best pH/temperature was obtained at pH 5 and 70 °C, with Km and V max values of chitinase to be 5.6 mg/mL and 0.87 µmol/min, respectively. Ag+, Co2+, iodoacetamide, and iodoacetic acid inhibited the enzyme activity, whereas Mn2+, Cu2+, Tweens (20 and 80), Triton X-100, and EDTA increased the same. In addition, the study of the morphological alteration of chitin treated by enzyme by SEM revealed cracks and pores on the chitin surface, indicating a potential application of this enzyme in several industries.
Descritores: Bacillus/metabolismo
Quitinases/metabolismo
-Fósforo/metabolismo
Temperatura Ambiente
Bacillus/isolamento & purificação
Bacillus/genética
Bacillus/ultraestrutura
Estabilidade Enzimática/efeitos dos fármacos
Carbono/metabolismo
RNA Ribossômico 16S/genética
Cinética
Quitinases/química
Análise de Sequência de DNA
Ativação Enzimática
Concentração de Íons de Hidrogênio
Íons
Metais
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


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Id: lil-788980
Autor: El-Gendy, Mervat Morsy Abbas Ahmed; El-Bondkly, Ahmed Mohamed Ahmed.
Título: Evaluation and enhancement of heavy metals bioremediation in aqueous solutions by Nocardiopsis sp. MORSY1948, and Nocardia sp. MORSY2014
Fonte: Braz. j. microbiol;47(3):571-586, July-Sept. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT An analysis of wastewater samples collected from different industrial regions of Egypt demonstrated dangerously high levels of nickel (0.27-31.50 mg L-1), chromium (1.50-7.41 mg L-1) and zinc (1.91-9.74 mg L-1) in the effluents. Alarmingly, these heavy metals are among the most toxic knownones to humans and wildlife. Sixty-nine Actinomycete isolates derived from contaminated sites were evaluated under single, binary, and ternary systems for their biosorption capacity for Ni2+, Cr6+ and Zn2+ from aqueous solutions. The results of the study identified isolates MORSY1948 and MORSY2014 as the most active biosorbents. Phenotypic and chemotypic characterization along with molecular phylogenetic evidence confirmed that the two strains are members of the Nocardiopsis and Nocardia genera, respectively. The results also proved that for both the strains, heavy metal reduction was more efficient with dead rather than live biomass. The affinity of the dead biomass of MORSY1948 strain for Ni2+, Cr6+ and Zn2+ under the optimized pH conditions of 7, 8 and 7, respectively at 40 °C temperature with 0.3% biosorbent dosage was found to be as follows: Ni2+ (87.90%) > Zn2+ (84.15%) > Cr6+ (63.75%). However, the dead biomass of MORSY2014 strain under conditions of pH 8 and 50 °C temperature with 0.3% biosorbent dose exhibited the highest affinity which was as follows: Cr6+ (95.22%) > Ni2+ (93.53%) > Zn2+ (90.37%). All heavy metals under study were found to be removed from aqueous solutions in entirety when the sorbent dosage was increased to 0.4%.
Descritores: Metais Pesados/metabolismo
Nocardia/classificação
Nocardia/metabolismo
-Temperatura Ambiente
Fatores de Tempo
Poluentes Químicos da Água/metabolismo
Biodegradação Ambiental
RNA Ribossômico 16S/genética
Biomassa
Metais Pesados/toxicidade
Adsorção
Egito
Águas Residuais/microbiologia
Concentração de Íons de Hidrogênio
Resíduos Industriais
Nocardia/isolamento & purificação
Nocardia/genética
Responsável: BR1.1 - BIREME


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Id: lil-788974
Autor: Gururaj, P; Ramalingam, Subramanian; Devi, Ganesan Nandhini; Gautam, Pennathur.
Título: Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07
Fonte: Braz. j. microbiol;47(3):647-657, July-Sept. 2016. tab, graf.
Idioma: en.
Projeto: Department of Biotechnology, Government of India.
Resumo: ABSTRACT The purpose of this study was to isolate, purify and optimize the production conditions of an organic solvent tolerant and thermostable lipase from Acinetobacter sp. AU07 isolated from distillery waste. The lipase production was optimized by response surface methodology, and a maximum production of 14.5 U/mL was observed at 30 ºC and pH 7, using a 0.5% (v/v) inoculum, 2% (v/v) castor oil (inducer), and agitation 150 rpm. The optimized conditions from the shake flask experiments were validated in a 3 L lab scale bioreactor, and the lipase production increased to 48 U/mL. The enzyme was purified by ammonium sulfate precipitation and ion exchange chromatography and the overall yield was 36%. SDS-PAGE indicated a molecular weight of 45 kDa for the purified protein, and Matrix assisted laser desorption/ionization time of flight analysis of the purified lipase showed sequence similarity with GDSL family of lipases. The optimum temperature and pH for activity of the enzyme was found to be 50 ºC and 8.0, respectively. The lipase was completely inhibited by phenylmethylsulfonyl fluoride but minimal inhibition was observed when incubated with ethylenediaminetetraacetic acid and dithiothreitol. The enzyme was stable in the presence of non-polar hydrophobic solvents. Detergents like SDS inhibited enzyme activity; however, there was minimal loss of enzyme activity when incubated with hydrogen peroxide, Tween 80 and Triton X-100. The kinetic constants (Km and Vmax) revealed that the hydrolytic activity of the lipase was specific to moderate chain fatty acid esters. The Vmax, Km and Vmax/Km ratio of the enzyme were 16.98 U/mg, 0.51 mM, and 33.29, respectively when 4-nitrophenyl palmitate was used as a substrate.
Descritores: Compostos Orgânicos
Solventes
Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/biossíntese
Acinetobacter/enzimologia
Lipase/isolamento & purificação
Lipase/biossíntese
-Compostos Orgânicos/química
Solventes/química
Especificidade por Substrato
Temperatura Ambiente
Proteínas de Bactérias/química
Estabilidade Enzimática
Cinética
Cromatografia por Troca Iônica
Ativação Enzimática
Espaço Extracelular/enzimologia
Concentração de Íons de Hidrogênio
Íons
Lipase/química
Lipólise
Metais
Peso Molecular
Responsável: BR1.1 - BIREME



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